Effects of Prudhoe bay crude oil on primary production and zooplankton in arctic tundra thaw ponds

The effects of Prudhoe Bay, Alaska, crude oil on the indigenous phytoplankton and zooplankton of tundra thaw ponds were studied under controlled conditions in situ during the summer of 1976. These effects were compared with uncontrolled oil spills on Pond Omega (a year previously) and Pond E (six years previously). In the uncontrolled spills, the phytoplankton species composition of both ponds remained appreciably different compared with control Pond C, although phytoplankton biomass did not differ greatly. Primary production remained low in Pond Omega but had recovered to control levels in Pond E. In controlled subpond experiments, oil caused a decrease of about 90–100% in primary production in five days but recovered to 40–50% of the control level within fifteen days. During that time, phytoplankton biomass decreased initially but recovered within fifteen days. Oil caused a shift in phytoplankton species composition from a predominance of cryptophytes to chrysophytes. Subponds containing two Daphnia middendorffiana and one Brachinecta paludosa per litre of pondwater were also affected by oil, causing zooplankton death within three or four days. After that time, changes in the phytoplankton species composition were similar to control subponds without zooplankton. Oil toxicity to zooplankton or experimental removal resulted in a loss of grazing pressure which caused the elimination of the cryptophyte Rhodomonas sp. This species was still absent from Pond Omega, but was seen in Pond E for the first time, when zooplankton also reappeared after six years. Oil perturbation of tundra thaw ponds causes a loss of zooplankton and a reduction in primary production. Phytoplankton primary production recovers somewhat but algal species composition remains changed because of the loss of zooplankton grazing pressure and the selective effects of oil.     

An experimental marine ecosystem study of the pelagic biogeochemistry of pentachlorophenol

The marine biogeochemistry of pure pentachlorophenol was studied relative to a control treatment at a scale which provided a good model for the pelagic planktonic environment (70 m³ polyethylene bags). Pentachlorophenol that was dispersed at concentrations of 10 and 100 μg/litre showed identical relative rates of decrease over 25 days to concentrations 32·8% and 31·1%, respectively, of the initial concentrations. No bioaccumulation by phytoplankton or zooplankton was observed and pentachlorophenol removal by adsorption (by particulates, zooplankton or the enclosure walls) was insignificant. The log of the pentachlorophenol concentration was significantly correlated to the hours of bright sunshine up to Day 18, after which the photolysis efficiency decreased as the proportion of pentachlorophenol exposed to sunlight decreased. The results imply a long residence time for pentachlorophenol in deeper waters outside the photolysis zone. A persistent reduction in the primary production and production per unit carbon was observed at 100 μg/litre and, when coupled to the reproducible demise of the centric diatom Skeletonema costatum in culture and the spiked enclosures, suggests that pentachlorophenol inhibits phytoplankton growth.     

Modelling growth and reproduction of the Pacific oyster Crassostrea gigas: Advances in the oyster-DEB model through application to a coastal pond

A bio-energetic model, based on the DEB theory exists for the Pacific oyster Crassostrea gigas. Pouvreau et al. [Pouvreau, S., Bourles, Y., Lefebvre, S., Gangnery, A., Alunno-Bruscia, M., 2006. Application of a dynamic energy budget model to the Pacific oyster, C. gigas, reared under various environmental conditions. J. Sea Res. 56, 156–167.] successfully applied this model to oysters reared in three environments with no tide and low turbidity, using chlorophyll a concentration as food quantifier. However, the robustness of the oyster-DEB model needs to be validated in varying environments where different food quantifiers reflect the food available for oysters, as is the case in estuaries and most coastal ecosystems. We therefore tested the oyster-DEB model on C. gigas reared in an Atlantic coastal pond from January 2006 to January 2007. The model relies on two forcing variables: seawater temperature and food density monitored through various food quantifiers. Based on the high temperature range measured in this oyster pond (3–30 °C), new boundary values of the temperature tolerance range were estimated both for ingestion and respiration rates. Several food quantifiers were then tested to select the most suitable for explaining the observed growth and reproduction of C. gigas reared in an oyster pond. These were: particulate organic matter and carbon, chlorophyll a concentration and phytoplankton enumeration (expressed in cell number per litre or in cumulative cell biovolume). We conclude that when phytoplankton enumeration was used as food quantifier, the new version of oyster-DEB model presented here reproduced the growth and reproduction of C. gigas very accurately. The next step will be to validate the model under contrasting coastal environmental conditions so as to confirm the accuracy of phytoplankton enumeration as a way of representing the available food that sustains oyster growth.     

The influence of dissolved petroleum hydrocarbon residues on natural phytoplankton biomass

The effect of dissolved petroleum hydrocarbons in the environment on phytoplankton biomass measured as chlorophyll a was studied near the oil tanker route in the southern Bay of Bengal. In the transect from 5° N, 77° E to 5° N, 87° E the concentrations of dissolved petroleum hydrocarbons were negatively correlated with phytoplankton biomass, whereas in the 0° N, 87° E to 1° N, 79° E transect they were positively correlated with phytoplankton biomass. The mean petroleum hydrocarbon concentrations in the two transects were 12·12 ± 4·67 μg litre⁻¹ and 11·23 ± 4·5 μg litre⁻¹, respectively.It is surmised that the effect of dissolved petroleum hydrocarbons on phytoplankton biomass varies depending on the nature rather than the quantity of petroleum hydrocarbons present. Culture studies with unialgal Nitzschia sp. in seawater collected from selected stations in the study area as well as in artificial seawater spiked with the water-soluble petroleum hydrocarbon fraction of light Arabian Crude support this.     

“底充式增氧”技术对梭子蟹养殖池塘水质环境及浮游植物群落结构的影响

于2009年6-9月在浙江省宁海县三门湾海域养殖场,选取2口水文环境相似的梭子蟹Portunus trituberculatus养殖池塘,分别采用“底充式增氧”(实验塘)和传统水车式增氧(对照塘)方式,比较研究了池塘水质环境及其浮游植物群落结构的变化.结果显示:(1)实验塘水质的溶解氧和氨氮指标均优于对照塘,其中实验塘...     

荣成靖海湾海参养殖池塘初级生产力季节变化特征

2007年5月~2007年11月调查了山东荣成靖海湾海参养殖池塘水体中浮游植物种类组成、生物量、叶绿素含量变化以及底泥沉积物中叶绿素和脱镁叶绿素含量的变化.结果表明,海参养殖池塘水体中浮游植物共有7门,44属,62种,主要以硅藻为主.水体中浮游植物平均生物量1号池塘为17.52×10~4 /L;2号池塘为18.30×10~4 /L.水体叶绿素含量变化范围:1号池塘为1.38～5.00 μg/L;2号池塘为0.98～5.45 μg/L.池塘沉积物中叶绿素含量变化范围:1号池塘为0.66～1.67 μg/g,2号池塘为0.56～1.34 μg/g.池塘沉积物中脱镁叶绿素含量变化范围:1号池塘为2.97～5.63 μg/g;2号池塘为2.90～6.36 μg/g.1号池塘和2号池塘的浮游植物群落结构及叶绿素含量没有显著性差异(P>0.05).叶绿素,脱镁叶绿素变化趋势较为一致.海参夏眠期间,沉积物中叶绿素及脱镁叶绿素含量最高,为海参结束夏眠进入摄食提供食物储备.研究结果表明,靖海湾海参养殖池塘浮游植物以硅藻为主,养殖池塘水体浮游植物生物量较低,多样性指数较高,增加浮游植物沉降速率,增加养殖系统初级生产力可为海参提供更多食物.     

Seasonal phytoplankton composition, productivity and biomass in the Neuse River estuary, North Carolina

Phytoplankton community composition, productivity and biomass characteristics of the mesohaline lower Neuse River estuary were assessed monthly from May 1988 to February 1990. An incubation method which considered water-column mixing and variable light exposure was used to determine phytoplankton primary productivity. The summer productivity peaks in this shallow estuary were stimulated by increases in irradiance and temperature. However, dissolved inorganic nitrogen loading was the major factor controlling ultimate yearly production. Dynamic, unpredictable rainfall events determined magnitudes of seasonal production pulses through nitrogen loading, and helped determine phytoplankton species composition. Dinoflagellates occasionally bloomed but were otherwise present in moderate numbers; rainfall events produced large pulses of cryptomonads, and dry seasons and subsequent higher salinity led to dominance by small centric diatoms. Daily production was strongly correlated (r = 0·82) with nitrate concentration and inversely correlated (r = −0·73) with salinity, while nitrate and salinity were inversely correlated (r = −0·71), emphasizing the importance of freshwater input as a nutrient-loading source to the lower estuary. During 1989 mean daily areal phytoplankton production was 938 mgC m⁻², mean chlorophyll a was 11·8 mg m⁻³, and mean phytoplankton density was 1·56 × 10³ cells ml⁻¹. Estimated 1989 annual areal phytoplankton production for the lower estuary was 343 gC m⁻².     

固城湖生物资源利用和富营养化控制的研究

于１９８９－１９９２年通过野外调查、室内试验，池塘模拟和数学模型等方法。研究江苏省固城湖的理化，初级生产生物生长及其有关因子，水草、藻和鱼，蟹间的相互关系，以及水草渔业利用对水质的影响等。     

Physiological responses of a marine fish exposed to chlorinated seawater at concentrations near its avoidance threshold

To determine if avoidance of chlorinated seawater by fish resulted in physiological protection from toxicity, studies were carried out which assessed (a) changes in the routine oxygen consumption rate and (b) the ability of treated fish to successfully compete with untreated conspecifics for a limited food resource.Temperate marine damselfish, Chromis punctipinnis, were exposed to stepwise increasing levels of chlorinated seawater in a behaviour chamber and avoided total residual oxidant (TRO) levels greater than 0·15-0·16 mg/litre. Cumulative exposures ranged from the equivalent of 0·38-5·23 in at 1·0/litre TRO. One day after exposure, routine oxygen consumption rates were decreased by 25 to 45% from pre-exposure rates and were correlated with the cumulative oxidant exposure. One month post exposure, respiration rates returned to pre-treatment levels. This transient depression of the respiratory rate did not affect survival or growth of chlorine-treated fish which were forced to compete with untreated conspecifics for a restricted food supply.     

Longwaves and primary productivity variations in the equatorial pacific at 0°, 140dgW

High temporal resolution measurements of physical and bio-optical variables were made in the upper ocean using a mooring located at 0°, 140°W from 9 February 1992 to 15 March 1993 as part of the equatorial Pacific Ocean (EgPac) study. Chlorophyll and primary productivity time-series records were generated using the mooring data. Primary productivity varied by about 50% around the mean on time scales of weeks and by over a factor of four within our observational period. The mooring observations encompassed both El Niho and cool conditions. Kelvin waves were evident during the El Nifio phase, and tropical instability waves (TIWs) were dominant during the cool phase. The two extreme conditions also were observed concurrently with complementary ship-based measurements. In addition, bio-optical drifters provided simultaneous spatial data concerning net phytoplankton growth rates during passage of a TIW. The collective data sets have been used to examine the causes of the observed variability in phytoplankton biomass and productivity. Our joint results and analyses appear to support the hypothesis that the vertical transport of iron into the upper layer and primary production rates are modulated by variability of the depth of the Equatorial Undercurrent and by equatorial longwaves. In particular, our results are consonant with the suggestion of Barber et al. (1996) that passage of a TIW may be considered to be a natural analog of a small iron enrichment experiment. Predicting primary productivity and, thus, carbon flux in the equatorial Pacific requires continuous, long-term observations of a few physical, biological, and optical properties that can be used to parameterize the biological variability.     

Toxicity of copper to three estuarine bivalves

This paper reports on a study of the toxicity of copper to three species of bivalve of commercial food value, Anadara granosa (Linnaeus), Meretrix casta, Deshayes and Crassostrea madrasensis (Preston) inhibiting the Vellar estuary at Porto Novo (southern India). The LC₅₀ values were estimated as 60 μg Cu/litre for A. granosa, 72 μg Cu/litre for M. casta and 88 μg Cu/litre for C. madrasensis subjected to 96-h static bioassay tests after acclimation for a period of four days in the laboratory. The animals selected for study ranged in length from 29 to 44 mm in A. granosa, from 25 to 42 mm in M. casta and from 31 to 115 mm in C. madrasensis. The experiments were conducted at 25% salinity with pH 8·0 ± 0·1 at a temperature of 27°C ± 0·5°C. The revival rates of bivalves which survived exposure to the LC₅₀ concentrations for the 96-h period (when marked and released in the natural environment) were 67% for A. granosa, and C. madrasensis and 83% for M. casta. The LT₅₀ values for the three bivalves at different concentrations of the metal were also calculated.     

Seasonal Variations in Benthic Community Metabolism and Nitrogen Dynamics in a Shallow, Organic-Poor Danish Lagoon

The dynamics of benthic primary production and community respiration in a shallow oligotrophic, marine lagoon (Fællestrand, Denmark) was followed for 1·5 years. The shape of the annual primary production cycle was explained primarily by seasonal changes in temperature (r² = 0·67-0·72) and daylength (r² = 0·63), whereas temperature almost explained all variation in benthic community respiration (r² = 0·83-0·87). On a daily basis the benthic system was autotrophic during spring and summer supplied by 'new' and 'regenerated' nitrogen and predominantly heterotrophic during fall and winter caused by light and nutrient limitation. The linear depth-relationship between porewater alkalinity and ammonium indicated that the C:N ratio of mineralized organic matter is low in spring and summer (3-6) and high in fall and winter (9-16). This is inversely related to net primary production and thus the input of labile, nitrogen-rich algal cells. Accordingly, mineralization occurred predominantly in the upper 2-5 cm of the sediment. The pool of reactive material (microalgal cells) was estimated to account for 12% of total organic carbon in the upper 3 cm, and had an average turnover time of less than 1 month in summer. Assimilation of organic carbon by benthic animals was equivalent to about 30% of the annual gross primary production. Grazing reduced chlorophyll a concentration in the sediment during summer and spring to values 30-40% lower than in winter, but maintained a 3-4 times higher specific microalgal productivity. The rapid turnover of organic carbon and nitrogen, and important role of benthic microalgae showed that the benthic community in this oligotrophic lagoon is of a very dynamic nature.     

The relationship between the copper complexing capacity of sea water and copper toxicity in shrimp zoeae

Mortality of larval coon-stripe shrimp, Pandalus danae, was related to labile copper and the copper complexing capacity of sea water, as measured by differential pulse Anodic Stripping Voltammetry (ASV). In nominal treatments of control, 5 and 10μg/litre Cu which contained less than 1·0μg/litre ASV labile Cu, zoeal development was usually complete. In treatments of 20 and 50μg/litre Cu which contained more than 7·0μg/litre ASV labile Cu, most larvae died while in the first zoeal stage. ASV labile Cu was related to the copper complexing capacity of sea water. Copper toxicity at less than 1·0 μg/litre ASV labile Cu is indicated by moulting delay and apparent Cu accumulation in zoea of the 5 and 10μg/litre Cu nominal treatments.     

Laboratory study of nitrogen and phosphorus remineralization during the decomposition of coastal plankton and seston

The decomposition of cultured marine phytoplankton (Skeletonema costatum) and natural estuarine seston from Narragansett Bay, RI, was studied at two temperatures (8°C and 18°C) in bottles containing sterile bay-water (30‰) and in bay-water with micro-organisms small enough to pass through a glass fibre filter (nominally < 1μ). About 50% of the particulate organic nitrogen (PON) and particulate phosphorus (PP) was immediately released to the water in dissolved organic forms from both types of organic matter. Comparison of changes in the dissolved organic nitrogen (DON) fraction in the sterile and non-sterile systems indicated that nearly all of the DON initially released was subsequently remineralized. Ammonification proceeded only in non-sterile bay-water. 20–25% of the PP was converted to dissolved inorganic-P (DIP) fraction after only 7 h in both sterile and non-sterile bay-water. Following autolytic releases of DON, DOP and DIP the initial rates of N and P remineralization were temperature dependent: Q₁₀ values for PON and PP decay during first phase of microbially mediated decomposition ranged from 1·3 to 6·4. Rates of remineralization then slowed so that about equal amounts of nutrients were remineralized (45–50% of the N and 57–60% of the P in the phytoplankton and 60–63% of the N and 36–60% of the P in the natural seston) after 30 days storage at either temperature. During 30 days of decomposition in non-sterile seawater the N/P ratios in the dissolved inorganic fractions converged on the ratios of total-N/total-P initially present in the bottles. Kinetic analysis of the decay of total organic-N (TON) and total organic-P (TOP) in the non-sterile systems and analysis of similar sets found in the literature showed that the initial stages of the decomposition of N and P from planktonic POM in vitro could be modelled as the sequential decay, at first-order rates, of two particulate fractions. The first, more labile, fraction comprised about 60% of the particulate N and P. First-order rate constants (−k, base e) for decomposition during the 1st and 2nd phases were 0·02 to 0·2 day⁻¹ and 0·003 to 0·02 day⁻¹, respectively. The decay rates are far too slow to account for the ‘rapid in situ recycling’ of nutrients needed to support phytoplankton production when other means of nutrient resupply (by advection, fixation, rainfall, etc.) are very low.     

Trophic Status of the South-Eastern Baltic Sea: A Comparison of Coastal and Open Areas

Primary production, nutrient concentrations, phytoplankton biomass (incl. chlorophyll a) and water transparency (Secchi depth), are important indicators of eutrophication. Earlier basin-wide primary production estimates for the Baltic Sea, a shallow shelf sea, were based mainly on open-sea data, neglecting the fundamentally different conditions in the large river plumes, which might have substantially higher production. Mean values of the period 1993–1997 of nutrient concentrations (phosphate, nitrate, ammonium and silicate), phytoplankton biomass, chlorophyll a (chl a) concentration, turbidity and primary production were calculated in the plumes of the rivers Oder, Vistula and Daugava and Klaipeda Strait as well as the open waters of the Arkona Sea, Bornholm Sea, eastern Gotland Sea and the Gulf of Riga. In the plumes, these values, except for primary production, were significantly higher than in the open waters. N:P ratios in the plumes were >16 (with some exceptions in summer and autumn), indicating potential P-limitation of phytoplankton growth, whereas they were <16 in the open Baltic Proper, indicating potential N-limitation. On the basis of in situ phytoplankton primary production, phytoplankton biomass and nutrient concentrations, the large river plumes and the Gulf of Riga could be characterized as eutrophic and the outer parts of the coastal waters and the open sea as mesotrophic. Using salinity to define the border of the plumes, their mean extension was calculated by means of a circulation model. Taking into account the contribution of coastal waters, the primary production in the Baltic Proper and the Gulf of Riga was 42·6 and 4·3×10⁶ t C yr⁻¹, respectively. Hence, an annual phytoplankton primary production in the whole Baltic Sea was estimated at 62×10⁶ t C yr⁻¹. The separate consideration of the plumes had only a minor effect on the estimation of total primary production in comparison with an estimate based on open sea data only. There is evidence for a doubling of primary production in the last two decades. Moreover, a replacement of diatoms by dinoflagellates during the spring bloom was noticed in the open sea but not in the coastal waters. A scheme for trophic classification of the Baltic Sea, based on phytoplankton primary production and biomass, chl a and nutrient concentrations, is proposed.     

Sodium hexametaphosphate as an aid in benthic sample sorting

The efficacy of sodium hexametaphosphate, when added to a formalin-Rose Bengal fixative solution, in improving the efficiency of benthic sample sorting by encouraging the disaggregation of silt-clay sediment has been investigated. Samples of estuarine mud (Mdφ = 6·1) from Damhead Creek, River Medway, were used for the analysis. A significant increase (23%; p < 0·01) in sieving efficiency was found in samples treated with concentrations of 1·24g/litre or more of sodium hexametaphosphate; treatments with higher concentrations than 1·24g/litre did not improve on this performance significantly. The use of 1·24g/litre sodium hexametaphosphate in an initial fixative/preservative solution is recommended as an economic and practical means of improving the efficiency of benthic sample sorting, without deleterious effects on the animal specimens in the sample.     

Acute toxicity of bromochlorinated seawater to selected estuarine species with a comparison to chlorinated seawater toxicity

The acute toxicity of bromochlorinated estuarine water (ca. 20%) was determined for several estuarine organisms. The most sensitive species were oysters (Crassostrea virginica, larvae and juveniles) and copepods (Acartia tonsa) with 48-h LC₅₀'s of 0·10 to 0·21 mg BrCl/litre. Palaemonetes pugio was most tolerant with a 96-h LC₅₀ of 0·70 mg BrCl/litre. The fish species tested (Menidia menidia, Brevoortia tyrannus and Leiostomus xanthurus) all had a 96-h LC₅₀ of 0·21–0·23 mg BrCl/litre.The BrCl toxicity data are compared with Cl₂ toxicity data for the same species. When the LC₅₀'s are expressed as equivalents per litre, BrCl is found to be two to four times less toxic than Cl₂. The ranking of species in terms of sensitivity is the same for both disinfectants.Some data are provided concerning the decay rates of BrCl and Cl₂ in estuarine water. BrCl was found to decay more rapidly than Cl₂ at higher ammonia levels (0·25 mg NH₄-N/litre). The question of chemical speciation is discussed with particular reference to the differential toxicities.     

Assessing temporal variation of coloured dissolved organic matter in the coastal waters of South Eastern Arabian Sea

Coloured dissolved organic matter （CDOM） plays a major role in marine photochemical and biological processes and its optical properties are known to affect the underwater light penetration. This paper highlights in situ optical estimation and satellite retrieval of CDOM in deciphering its temporal variations in coastal waters of the South Eastern Arabian Sea. The study accentuated the source of CDOM as terrigenous origin during monsoon, of in situ productions during pre-monsoon and during post-monsoon of autochthonous-allocthonous origin. The matchup analysis for in situ and MODIS Aqua retrieved A_dg443 exhibited bias which decreased by incorporating the seasonal component. The study also identified degrading bloom of Noctiluca scintillans as the source for exceptionally high CDOM in the area during January and February. The study demands to incorporate seasonal components and phytoplankton abundance while assessing the performance of CDOM algorithms in optically complex coastal waters.     

养水机工作时长对参池氮磷营养盐及初级生产力的影响

采集以6 h、12 h、18 h养水机工作时长下的刺参(Stichopus japonicus)养殖池塘水样,测定无机氮、磷酸盐浓度和初级生产力毛、净产量,并对无机氮组成占比、氧化参数和P/R系数(初级生产力毛产量与自养呼吸量之比)进行了测算,以探究养水机工作时长对池塘氮、磷营养盐和初级生产力的影响.结果显示,延长养水...     

Influence of upwelling and river runoff interaction on phytoplankton assemblages in a Middle Galician Ria and Comparison with northern and southern rias (NW Iberian Peninsula)

The first oceanographic research (hydrography, nutrient salts, chlorophyll, primary production and phytoplankton assemblages) in a Middle Galician Ria was carried out in Corme-Laxe during 2001, just a year before the Prestige oil spill, being the only reference to evaluate eventual changes in the phytoplankton community. Due to the small size of this ria (6.5 km²), oceanographic processes were driven by the continental water supplied by Anllons River during the wet season (20–30 m³ s⁻¹ in winter), and the strong oceanic influence from the nearby shelf during the dry season. The annual cycle showed a spring bloom with high levels of chlorophyll (up to 14 μg Chl-a L⁻¹) and primary production (3 g C m⁻² d⁻¹) and a summer upwelling bloom (up to 8 μg Chl-a L⁻¹ and 10 g C m⁻² d⁻¹) where the proximity of the Galician upwelling core (<13.5 °C at sea surface) favors the input of upwelled seawater (up to 9 μM of nitrate and silicate) to the bottom ria layer, even during summer stratification events (primary production around 2 g C m⁻² d⁻¹). Thus, phytoplankton assemblages form a “continuum” from spring to autumn with a predominance of diatoms and overlapping species between consecutive periods; only in autumn dinoflagellates and flagellates characterized the phytoplankton community. In the Middle Rias as Corme-Laxe, the nutrient values, Chl-a, primary production and phytoplankton abundance for productive periods were higher than those reported for the Northern (Ria of A Coruña) and Southern Rias (Ria of Arousa) for year 2001; this suggests the importance of the hydrographic events occurring in the zone of maximum upwelling intensity of the Western Iberian Shelf, where a lack of annual cycles studies exists.