<Emphasis Type="Italic">Cochlodinium polykrikoides</Emphasis> red tide detection in the South Sea of Korea using spectral classification of MODIS data

To distinguish true red tide water (particularly Cochlodinium polykrikoides blooms) from non-red tide water (false satellite high chlorophyll water) in the South Sea of Korea, we developed a systematic classification method using spectral information from MODIS level products and applied it to five different harmful algal bloom events. Red tide and nonred tide waters were classified based on four different criteria. The first step revealed that the radiance peaks of potential red tide water occurred at 555 and 678 nm. The second step separated optically different waters that were influenced by relatively low and high contributions of colored dissolved organic matter (CDOM) (including detritus) to chlorophyll. The third and fourth steps discriminated red tide water from non-red tide water based on the blue-to-green ratio in areas with lower and higher contributions of CDOM to chlorophyll, respectively. After applying the red tide classification (using the four criteria), the spectral response of the red tide water, which is influenced by pigment concentration, showed different slopes for the blue and green bands (lower slope at blue bands and higher slope at green bands). The opposite result was found for non-red tide water, due to decreasing phytoplankton absorption and increasing detritus/CDOM absorption at blue bands. The results were well matched with the discoloration of water (blue to dark red/brown) and delineated the areal coverage of C. polykrikoides blooms, revealing the nature of spatial and temporal variations in red tides. This simple spectral classification method led to increase user accuracy for C. polykrikoides and non-red tide blooms (>46% and >97%) and provided a more reliable and robust identification of red tides over a wide range of oceanic environments than was possible using chlorophyll a concentration, chlorophyll anomaly, fluorescence analysis, or proposed red tide detection algorithms.     

Estimation of chlorophyll concentration from the specific fluorescence at 685nm in the upward irradiance just below the sea surface

An algorithm for estimating the chlorophyll concentration from the fluorescence intensity at 685nm in upward irradiance observed just below the sea surface is presented and discussed. The observed chlorophyll concentration is closely related to the ratio of the fluoresced quanta to the total incident quanta (downward plus upward quanta) in the spectral range between 350nm and 600nm. The correlation between them, however, is not good and the rate of increase of the ratio decreases as the chlorophyll concentration increases. This is due to the fact that the number of fluoresced quanta varies with attenuation of the incident light by various materials in the sea.A better correlation is obtained between the ratio of the fluoresced quanta to the upward quanta at wavelengths around 480nm. This is explained by the fact that dividing by the upward quanta at the appropriate wavelength compensates for apparent variation of fluorescence intensity with attenuation by the sea water. In this method, upward quanta at the four wavelengths of 480nm, 640nm, 685nm and 740nm are necessary for estimation of chlorophyll concentration. The quanta at 640nm and 740nm are used for interpolating the quanta at 685nm in the absence of fluorescence.It is also shown that the correlation of the observed chlorophyll concentration with the ratio of upward quanta at the wavelengths of 685nm and 480nm is also good. Use of this ratio is recommended for remote sensing of chlorophyll concentration since the number of spectral channels required by the remote multispectral imager can be minimized.     

舟山海域陆源溶解有机质变化及其对近岸羽状流的响应

2007年夏季在东海舟山海域河口锋区开展了陆源溶解有机质的调查研究。测定了有色溶解有机质(CDOM)在激发波长370 nm/发射波长460 nm处的荧光强度和在λ=355 nm处的吸收系数,用于代表陆源CDOM浓度,并测定了荧光指数以指示CDOM来源。结果表明,CDOM的荧光值和紫外吸收系数之间呈显著正相关性,陆源CDOM浓度大体有向海方向降低的趋势,但是纵向上存在一些"突跃"现象。在舟山海域东北角不时观测到表层水体含有高浓度的CDOM,但变异性很大,推测可能该海区受到长江口羽状流的影响。在连续观测站发现陆源CDOM浓度在低平潮时往往比高平潮时要高。河海水在混合过程中CDOM浓度与盐度呈显著的线性负相关关系。在低盐度的悬沙锋区(S<24)CDOM浓度明显低于理论稀释值,而在较高盐度的羽状锋区,CDOM浓度接近于理论稀释值。在盐度为24~31范围内,大部分水样的荧光指数在1.50上下波动,表明其中CDOM来源以陆地来源为主;在较低盐度(S<24)的水样中荧光指数在1.70至1.90以上,表明CDOM以海洋来源为主,这与其陆源组分在高浊度的低盐度区存在显著的去除过程有关。研究表明,舟山海域水质存在着显著的变异性,与近岸羽状流密切相关,陆源溶解有机质的分布特征对此有较好的响应。     

基于荧光猝灭原理的溶解氧分析仪的研制及其应用

研制出一种对溶解氧敏感的传感膜,其荧光材料主要是邻菲咯啉钌。由光源发出的中心波长为465 nm的强光照射到铺有该络合物的膜片上,激发出中心波长为620 nm的荧光,水体中的溶解氧会对激发出的荧光产生猝灭效应,猝灭程度与溶解氧的质量浓度之间存在线性关系。该膜片在无氧水中的荧光强度是空气饱和水中的20倍,变化较为明显。对比检测荧光信号在待测水体中猝灭前后的强度,即可测出水体中溶解氧的质量浓度。基于这一原理和荧光测量技术研制了光学溶解氧分析仪,利用该仪器对6处不同水域的天然水体进行测试,所得测试结果与国标碘量法的测试结果之间不存在显著性差异,误差≤2%,响应时间<1 min。在海水中30 m深处进行36h稳定性考察,所得溶解氧质量浓度数值平均偏差小于0.2 mg.L-1,证实仪器可适应较为恶劣的环境,且稳定性较好。     

Raman scattering and fluorescence spectra of water from the sea surface microlayer

The Raman scattering and fluorescence spectra were first obtained for the water of sea surface microlayers (SML) of 1 and 0.2 mm thickness (sampled with a Garrett net and a Lapshin capillary sampler, respectively). For reference, samples of water below the SML (from the layer of 0.5 m) were also taken. Substantial differences were found for the values of the normalized intensity of the fluorescence (the number of photons from the volume unit in response to a unit of excitation) between the aqueous humic substances and, presumably, oil hydrocarbons and proteins. Some slight but analyzable differences in the shapes and location of spectral bands were also found. These latter allow one to determine the content of salts and the characteristics of complicated organic compounds in the SML and to compare them to those within the water volume.     

厦门湾有色溶解有机物的光吸收特性研究

研究了厦门湾九龙江河口区、西海域、同安湾及东侧水道海水中有色溶解有机物(CDOM)的光吸收特性,分析了CDOM的河口行为,并讨论了CDOM光吸收特性与其荧光性质之间的关系。结果表明,厦门湾表层海水CDOM光吸收系数a(355)的水平分布表现为河口区最高、东侧水道最低、西海域和同安湾介于两者之间,底层水a(355)的分布与表层基本相似,表明陆源河流输入是厦门湾CDOM的主要来源;a(355)的垂直分布为表层高于底层,主要受水文和生物因素控制。厦门湾表层水CDOM光谱斜率S的平均值介于0.014—0.018nm-1,但河口低盐度区S值较小,反映陆源腐殖质的影响。a(355)在河口混合中呈保守行为,表明CDOM具有良好的保守性质。CDOM的吸收系数a(355)与其荧光强度之间表现为较好的相关关系,指示可以用灵敏度更高的荧光方法来研究CDOM的分布和行为。     

MODIS水色产品在中国黄、东海海域应用研究

An extensive study collected in situ data along the Yellow Sea(YS) and East China Sea(ECS) to assess the radiometric properties and the concentration of the water constituents derived from Moderate Resolution Imaging Spectroradiometer(MODIS). Thirteen high quality match-ups were obtained for evaluating the MODIS estimates of Rrs(λ), chlorophyll a(Chl a) and concentrations of suspended particulate sediment matter(SPM). For MODIS Rrs(λ), the mean absolute percentage difference(APD) was in the range of 20%–36%, and the highest uncertainty appeared at 412 nm, whereas the band ratio of Rrs(λ) at 488 nm compared with that at 547 nm was highly consistent, with an APD of 7%. A combination of near-infrared bands and shortwave infrared wavelengths atmosphere correction algorithm(NIR-SWIR algorithm) was applied to the MODIS data, and the estimation accuracy of Rrs were improved at most of the visible spectral bands except 645 nm, 667 nm and 678 nm. Two ocean-colour empirical algorithms for Chl a estimation were applied to the processed data, the results indicated that the accuracy of the derived Chl a values was obviously improved, the four-band algorithms outperformed the other algorithm for measured and simulated datasets, and the minimum APD was 35%. The SPM was also quantified. Two regional and two coastal SPM algorithms were modified according to the in situ data. By comparison, the modified Tassan model had a higher accuracy for the application along the YS and ECS with an APD of 21%. However, given the limited match-up dataset and the potential influence of the aerosol properties on atmosphere correction, further research is required to develop additional algorithms especially for the low Chl a coastal water.     

Tracing dissolved organic matter cycling in the eastern boundary of the temperate North Atlantic using absorption and fluorescence spectroscopy

Apparent oxygen utilization (AOU), dissolved organic carbon (DOC), coloured dissolved organic matter (CDOM) absorption spectra, and CDOM fluorescence characteristic of aromatic amino acids (excitation/emission 280 nm/320 nm; F(280/320)) and marine-humic like substances (320 nm/410 nm; F(320/410)) were measured in full depth during a cruise in the temperate Eastern North Atlantic (ENA). An optimum multi-parameter (OMP) inverse method was run to calculate water mass proportion-weighted average (archetypal) concentrations of these chemical parameters for all water masses and samples. Archetypal concentrations retain the variability due to water mass mixing and basin scale mineralization from the water mass formation sites to the study area. Conversely, the difference between measured and archetypal concentrations, retain the variability due to dissimilarities in mineralization processes within the study area. Our analysis indicates that DOC supported 26±3% of the AOU in the dark temperate ENA and that basin scale processes occurring at and from the formation area of the water masses explained 63% of the total DOC variability. Our data also suggests that DOC remineralized at the basin scale was of lower molecular weight, and with a lower proportion of fluorescent aromatic amino acids than found within the study area. The relationship between the absorption coefficient at 254 nm (a_CDOM(254)) and AOU indicates that a_CDOM(254) was consumed during organic matter remineralization in the dark ocean, with 55% of the variability being explained by basin scale processes. The relationships of F(320/410) with AOU and DOC confirmed that marine humic-like substances are produced by microbial degradation processes, at a rate of 6.1±0.9×10⁻³ mg equivalents of QS mol AOU⁻¹. Our results also indicate that basin-scale remineralization processes account for 85% of the total variability of F(320/410), emphasizing that large scale processes control the formation of humic-like substance in the dark ENA.     

Specific Features of the Vertical Distribution of the Beam Attenuation Coefficient in the Short- and Long-Wave Spectral Bands in Deep-Water Layers of the Hydrogen-Sulfide Zone and in the Bottom Layer of the Black Sea

On the basis of the data of sounding performed in the deep-water part of the sea down to 1920 m (for a depth of the sea of 2000 m), we study specific features of the vertical distribution of the beam attenuation coefficient in the violet (418 nm) and red (659 nm) spectral bands through the entire thickness of the hydrogen-sulfide zone including the bottom hydrologically homogeneous layer of water. The data of optical measurements performed in the bottom layer for the first time demonstrate that this layer is also optically uniform.     

西太平洋冬季上层水体有色溶解有机物的分布和转化特征

为深入解析西太平洋溶解有机碳的生物地球化学过程,本研究于2015年12月至2016年1月,开展了西太平洋上层水体有色溶解有机物（CDOM）吸收光谱和荧光光谱特征研究。研究结果表明,西太平洋上层水体CDOM吸收系数a（320）变化范围为0.01~1.07 m-1,平均值为0.18 m-1;其较高值位于100~200 m水层,表层的海水相对含量较低,主要以有机物的光化学分解为主。采用PARAFAC分析CDOM三维荧光光谱特征,得到1种类腐殖质组分C2（252（310 nm）/405 nm）及2种类蛋白组分C1（224（276 nm）/335 nm）和C3（224（260 nm）/300 nm）,其中类腐殖质荧光组分占总荧光强度的11%~22%,蛋白质荧光组分占总荧光强度的78%~89%,蛋白质荧光中类色氨酸和类络氨酸组分对荧光强度的贡献相当。洋流在大尺度上控制西太平洋CDOM的分布特征,两流交界处和环流形成区域的CDOM相对含量较高,荧光信号较强。西太上层水体CDOM相对含量和荧光信息,与温度、盐度、DO和营养盐等理化因素之间的相关分析结果表明,CDOM主要成分类蛋白质的产生主要受上层水体初级生产过程控制。     

Variability in the Relative Penetration of Ultraviolet Radiation to Photosynthetically Available Radiation in Temperate Coastal Waters, Japan

UVR and PAR wavelengths are attenuated to different extents within the water column, causing variations in spectral composition with depth. The present investigation (a) describes the variability of UVR and PAR penetration at a station in the temperate coastal waters of Sagami Bay and determines (b) the characteristics of relative UVR penetration to the euphotic zone. Examination of the seasonal irradiance profile measurements indicated eight measurements displaying two distinct attenuation coefficients (K _d) for specific UVR wavelengths and PAR. The two attenuation coefficients observed from specific wavelengths in the water column may be caused not only by chlorophyll pigments, but also by dissolved organic material in the upper layer. The 1% depth of surface UVR at 305, 320, 340, and 380 nm averaged 10.8 ± 5.7, 14.9 ± 9.5, 19.8 ± 12.1, and 30.4 ± 17.6 m, respectively. The depth of euphotic layer displayed less variability averaging 62 ± 15 m throughout the entire study. Relative UVR penetration within the euphotic zone averaged 17.8 ± 8.1, 22.9 ± 10, 30.5 ± 13.8, and 46 ± 46.9% for 305, 320, 340, and 380 nm, respectively. A large variation of the relative transmission of UVR within the euphotic zone was found although the spectral composition was relatively stable in the air throughout the study.     

钝顶螺旋藻C-藻蓝蛋白分子内不同基团间能量传递的研究

于１９９６年８－１２月,从钝顶螺旋藻中分离纯化Ｃ－藻蓝蛋白,并用荧光光谱法对Ｃ－藻蓝蛋白分子内不同基团间的能量传递进行研究,结果表明,Ｃ－藻蓝蛋白分子内芳香族氨基酸残基能将能量传至与脱辅基蛋白共价结合的色基,从而使色基产生相应的荧光,Ｃ－藻蓝蛋白分子２４０－２４５ｎｍ的荧光激发峰产生于二硫键,它也能将吸收的能量传至色基;同时还发现,溶液状态下的Ｃ－藻蓝蛋白分子内,色氨酸残基可能位于分子内部的疏水区     

渤海湾海水光谱特性的数值分析

自然界各种物体，都有表征其自然属性的光谱特性。地物的光谱特性是遥感图象定性判读的依据之一，它作为一种遥感信息也直接用于定量测定和制图。 海水中悬浮物质的数量和性质影响海水的颜色和海水的光谱特性。反之，根据海水的光谱特性可以测定水中悬浮物质的数量和性质。1974年， S. D. Duntley首先从海洋叶绿素的光谱效应，提出根据海水的光谱反射率曲线，用卫星传感器测定水中叶绿素a 浓度的理论。1978年， R. W. Johnson 建立了航空遥感的光谱数据与沿岸带海洋叶绿素a 浓度之间的数学关系，并绘制了叶绿素a的分布图。1979年， R. W. Johnson建立了光谱数据与悬浮泥沙含量之间的数学关系，并绘制了悬浮泥沙的定量分布图，等等。本文的目的即是要建立一种方法，用国产101W光谱辐射计记录的遥感数据，计算海域悬浮泥沙、叶绿素a 浓度、水色等水质参数。 1980年5月16日至19日，由中国科学院组织，有中国科学院海洋研究所、国家海洋局第一海洋研究所、第二海洋研究所、交通部天津水运科学研究所、水利部天津水利科学研究所等单位参加，在天津塘沽的海河和蓟运河口进行津、渤地区第一次环境污染航空遥感监测联合试验。在此次试验中用机载101W光谱辐射计记录海水的光谱辐射曲线，作者以此计算海水的光谱反射率曲线，用逐次回归分析法建立海洋实测数据(悬浮泥沙、叶绿素a浓度、水色等)与海水光谱反射率数据之间的数学关系。实验基本是成功的，由回归方程计算的结果与海洋实测数据之间的相关系数约为0.7。如果对研究海域的每一个子域的辐射值作光谱扫瞄，作出定量的分布图也是可行的。     

乌梁素海沉水植物群落光谱特征及其受覆盖度的影响分析

沉水植物是湖泊生态系统重要组成,也是水质与健康状况的指标性类群,可以采用遥感技术对其生长和分布情况进行监测.基于实测光谱数据,分析了乌梁素海沉水植物光谱特征,研究了覆盖度对乌梁素海沉水植物反射光谱的影响,建立了乌梁素海沉水植物覆盖度反演模型.结果 表明;沉水植物群落的光谱反射率随覆盖度减少而下降,覆盖度为1％～20％时...     

Characterization of dissolved organic matter fluorescence in the South Atlantic Bight with use of PARAFAC model: Interannual variability

Systematic water sampling for characterization of chromophoric dissolved organic matter (CDOM) in the coastal South Atlantic Bight, was conducted as part of the long term Coastal Ocean Research and Monitoring Program (CORMP). Water samples were collected during a 3.5 year period, from October 2001 until March 2005, in the vicinity of the Cape Fear River (CFR) outlet and in adjacent Onslow Bay (OB). During this study there were two divergent hydrological and meteorological conditions in the CFR drainage area: a severe drought in 2002, followed by the very wet year of 2003. CDOM was characterized optically by the absorption coefficient at 350 nm, the spectral slope coefficient (S), and by Excitation Emission Matrix (EEM) fluorescence. Parallel Factor Analysis (PARAFAC) was used to assess CDOM composition from EEM spectra and six components were identified: three terrestrial humic-like components, one marine humic-like component and two protein-like components. Terrestrial humic-like components contributed most to dissolved organic matter (DOM) fluorescence in the low salinity plume of the CFR. The contribution of terrestrial humic-like components to DOM fluorescence in OB was much smaller than in the CFR plume area. Protein-like components contributed significantly to DOM fluorescence in the coastal ocean of OB and they dominated DOM fluorescence in the Gulf Stream waters. Hydrological conditions during the observation period significantly impacted both concentration and composition of CDOM found in the estuary and coastal ocean. In the CFR plume, there was an order of magnitude difference in CDOM absorption and fluorescence intensity between samples collected during the drought compared to the wet period. During the drought, CDOM in the CFR plume was composed of equal proportions of terrestrial humic-like components (ca. 60% of the total fluorescence intensity) with a significant contribution of proteinaceous substances (ca. 20% of the total fluorescence). During high river flow, CDOM was composed mostly of humic substances (nearly 75% of total fluorescence) with minor contributions by proteinaceous substances. The impact of changes in fresh water discharge patterns on CDOM concentration and composition was also observed in OB, though to a lesser degree.     

Estimation of water quality parameters from irradiance reflectance using optical models

In order to evaluate near-surface water quality parameters from spectral irradiance reflectance observed just below the surface, models of both radiative transfer and optical properties of sea water are used. The computed water quality parameters are compared with those determined directly from concurrent surface water samples. In this comparison, efforts have been made to minimize the number of spectral irradiance reflectance used in the computation while ensuring that the computed water quality parameters fit well to the observed ones.The observed water quality parameters were found to be closely represented by those computed from the irradiance reflectance at wavelengths of 400 nm, 440 nm, 510 nm and 600 nm, and the fit is as good as the fit of observed water quality parameters to those computed from the irradiance reflectances at 151 wavelengths located between 400 nm and 700 nm at intervals of 2 nm. This implies that the water quality parameters can be evaluated from at least four radiances observed at high altitude, if the effects of the atmosphere and the sea surface can be correctly removed.     

Spatial and temporal variability of colored dissolved organic matter absorption properties in the Taiwan Strait

Spatial and temporal variability of the absorption properties of colored dissolved organic matter (CDOM) in the Taiwan Strait was investigated in summer (July to August of 2006) and winter (from December 2006 to January of 2007) seasons. The CDOM absorption coefficient at 280 nm (a 280 ) showed a decreasing trend from nearshore to offshore areas while the spectral slope coefficient parameter calculated between wavelengths 275-295 nm (S 275 295 ) showed an increase, indicative of decreasing aromaticity and molecular weight of the CDOM. The average a 280 in winter (1.47 ± 0.50 m 1 ) was significantly higher than in summer (1.10 ± 0.41 m 1 ), while the average S 275 295 in winter (26.7 ± 5.2 μm 1 ) was significantly lower than in summer (30.6 ± 5.5 μm 1 ), demonstrating clear seasonal variation in CDOM abundance and properties in the Taiwan Strait. A three-end- member conservative mixing model showed that local terrestrial CDOM inputs from several rivers along the western coast were small (<5%). However, the distribution of CDOM in the Taiwan Strait is mainly controlled by water mass movement [i.e., the Zhe-min Coastal Current (ZCC) and the Kuroshio Branch Current (KBC) in winter and the South China Sea Water (SCSW) in summer]. Biological activity was also an important factor affecting the distribution of CDOM in the offshore region in summer months.     

Tower-based radiometric observations at a coastal site in the Baltic Proper

An autonomous above-water radiometer was operated during the summer of 2005 on the Gustaf Dalén Lighthouse Tower (GDLT) off the Swedish coast in the Baltic Proper. Normalized water leaving radiances, L_WN(λ), produced from measurements performed with the autonomous system at various center-wavelengths λ in the 412–675 nm spectral range, were applied within the context of water quality monitoring and satellite ocean color validation activities. Specifically these in situ radiometric data were used to determine the chlorophyll a concentration through a regional band-ratio algorithm and to assess L_WN(λ) derived from top-of-atmosphere Moderate Resolution Imaging Spectroradiometer (MODIS) observations. The in situ measurements collected during a bloom occurring in July 2005 were also used to investigate the spectral and small scale temporal-spatial variability of L_WN(λ) in the presence of cyanobacteria.     

Photobleaching of fluorescence and the organic carbon concentration in a coastal environment

In order to investigate the photobleaching potential of estuarine waters from different depths and redox conditions and with varying degree of biological activity, filtered, unfiltered and chloroform-poisoned water samples from the Baltic Sea were exposed to ambient sunlight. Fluorescence, at excitation 350 nm and emission 450 nm, was used as an indication of humic substance concentration. Fluorescence and organic carbon concentration were measured at regular time intervals during light exposure. We found that the decrease in humic substance fluorescence can be fitted to an exponential decay function. The fluorescence half-lives were within the range 0.4 – 4.6 days in different water masses, with fluorescence decreasing to between 20% and 60% of initial concentration, respectively. Results from the curve fitting procedure indicate a rest concentration of humic substance fluorescence, similar among the sampled sites, that is resistant to further photochemical degradation. The largest relative decreases in fluorescence were found in deep waters, but samples from deep waters also had a higher fluorescence rest concentration than samples from surface waters. Biological activity was reduced by filtering the samples through 0.2μm pore size filters or adding chloroform. No statistically significant differences were found after 3 days of irradiation between samples with and without treatment to reduce biological activity. The highest initial fluorescence values and the largest fluorescence decrease were found in the anoxic waters of the Gotland Deep. The organic carbon concentrations decreased 3–7% at all stations. The shortest half-life of humic substance, and the largest decrease in organic carbon concentrations, were found in samples from the northern basins of the Baltic Sea.     

Optical Characteristics and Distribution of Chromophoric Dissolved Organic Matter in Onega Bay (White Sea) during the Summer Season (Findings from an Expedition from June 22 to 26, 2015)

Onega Bay waters are characterized by a high content of chromophoric dissolved organic matter (CDOM). The absorbance spectra and fluorescence intensity (excitation wavelength 455 nm, emission wavelength >680 nm) were used to assess the distribution of CDOM content in water filtered through a GF/F filter. The CDOM content at different points in Onega Bay showed more than a fourfold difference, as inferred from the measured values. The CDOM content in surface waters was, as a rule, higher than in the deeper horizons. A higher CDOM content was measured near the Onega River, near the middle part of the Onega shore, and near the Pomor shore opposite the town of Belomorsk. River runoff is the major source of CDOM in Onega Bay water. The CDOM chemical composition in Onega Bay waters was heterogeneous. The ratio of the fluorescence intensity to the absorbance value was higher near the mouths of rivers and in intensive mixing zones than in water characterized by high salinity. A highly significant linear correlation (R² = 0.7825) between water salinity and CDOM fluorescence intensity was demonstrated. The contribution of fluorescent compounds to river runoff CDOM is substantially higher than the contribution to the composition marine CDOM.