Degradation of linear alkylabenzene sulfonate (LAS) and its compounds in Donghu Lake (Hubei,P.R.C.) determined by high performance liquid chromatography (HPLC)

Commercial linear alkylbenzene sulfonate (LAS), mixture of alkylchain lengths and phenyl position isomers (C₁₀−C₁₃), is widely used as a major constituent of household and industrial detergents in the People's Republic of China. Degradation process and behaviour of LAS compounds during an 82-hour lake water die-away study, with an added LAS concentration of 1.5mg·L⁻¹, was quantified and accomplished by HPLO-UV after extractionon the SepPek C₁₈ reversed-phase cartridges. The degradation rate became progressively faster with increasing chain length. The technique described in this study is fast, sensitive and specific, and can be used to determine low levels of LAS and for establishing water quality criteria and standards relating to LAS and its compounds. Dr. Ayfer Yediler (GSF, National Center for Envirommental Sciences, Ingolstaedter Landstr. 1, D-8042 Neuherberg, FRG) is guest scientist in the Institute of Hydrobiology, Academia. Sinica, Wuhan.     

Rare earth elements in sediments of the China continental shelf

Determination of the concentrations of, 15 rare earth elements (REE) in China continental shelf sediments by X-ray fluorescent spectral analysis of selected representative sediment samples showed that REE concentration in the sediments is 156 ppm, similar to that in China loess and Fujian granite, but different from that in Pacific sediments. The shelf REE have the characteristic distribution pattern of typical continental crust REE and evidently philo-continental property. The above findings suggest that weathered materials transported from the China continent are the main source of the REE in China continental shelf sediments. Experiments show that REE exist mainly in the crystal lattices of clay minerals (<2μ) as isomorphs and that REE have close relation with most elements (Al, Ti, K, Rb, Fe, etc.) related with clay minerals, but have negative correlation with the biophile elements Ca and Sr. Contribution No. 1520 from the Institute of Oceanology, Academia Sinica.     

Simulated thermal alteration of sterols and keto—Alcohols in marine sediments of the Okinawa Trough

Compositional data on the sterol and alcohol fractions isolated from deep-sea marine sediments from the Okinawa Trough were obtained to determine the relative contribution from marine and terrestrial inputs. Following extraction, the sterol plus alcohol fraction was isolated by layer chromatography, derivatized with BSTFA and then analysed by capillary GC and GC—MS. A suite of C₂₆−C₂₉ stenols and stanols and C₃₀−C₃₂ keto—alcohols were identified in the sediments. The thermal stability of the compounds in these sediments was studied by heating portions of the surface sediment in glass tubes for 16 hours at temperatures from 50°C to 200°C. The C₂₇ stanol/stenol ratio increased when temperatures went up to 175°C, but the distribution of C₃₀−C₃₂ Keto—alcohols remained unaffected. At 200°C most of the sterols and Keto—alcohols were destroyed. Supported by the National Natural Foundation of China.     

A new species ofSolieria from China

A morphological study of a marine red alga from China, previously identified by various authors as “Solieria mollis (Harv.) Kylin”, has been shown to be an undescribed species ofSolieria. The present new speciesSolieria tenuis is characterized by the slender branches, the unbranched and nonseptate connecting filament and the discoid holdfast surrounded by prop branches resembling proproots of land plants. Contribution No. 989 from the Institute of Oceanology, Academia Sinica.     

Geochemical studies of U,Th, Ra,40K in seciments of the China Sea and adjacent sea area,and lower reaches of several Chinese Rivers

Geochemical studies of the U, Th, Ra, and⁴⁰K in surface sediments of the China Sea and adjacent sea areas, the lower reaches of the Huanghe and Changjiang Rivers and the estuaries of the Zhujiang and Rongjiang Rivers were carried out with a model 8180-4K multichannel Ge(Li)γ spectrometer in 1981–1985. The mean values of U, Ra, Th,⁴⁰K are about 2.11 ppm, 7.67×10⁻¹³g/g, 11.00 ppm, and 2.33 ppm respectively, in the China Sea, the Okinawa Trough, and the Changjiang and Huanghe River estuaries. The distribution of the four isotopes is uniform in the above zone. The contents of U and Th in the sediments of the Zhujiang and Rongjiang Rivers and the Fujian coast are 1–4 times higher than those of the above zone and are related to the granite and radioactive ore present in the upper reaches of these rivers or the adjacent mountains. The distributions of U, Th, Ra, and⁴⁰K are related to the physico-chemical property, redox state, geological type and grain size of the sediment, and biotic activity. Contribution No. 1408, Institute of Oceanology, Academia Sinica, Qingdao.     

Family Thraciidae (Mollusca,Bivalvia) from Chinese waters

Species,Thracia granubsa of the Family Thraciidae from the China Seas was first reported and described as new to science by Adams et Reeve (1850). No other species was found there for more than 100 years until the author (1980) in his studies on Chinese bivalvia, discovered the second new species,Trigonothracia jinxingae, described in this article. This paper deals with 6 species, three of which are new to science, collected from the littoral and sublittoral zones of the China Seas. Type specimens are deposited in the Institute of Oceanology, Academia Sinica, Qingdao. Contribution No. 1471 from the Institute of Oceanology, Academia Sinica.     

Two new species of Ophidiasterid sea-stars from the vicinity of Diaoyudao,East China Sea

Two new species of the asteroid family Ophidiasteridae are described from two specimens collected by commercial fishery vessels of Qingdao from the vicinity of Diaoyudao (25°45′N, 123°15′E), East China Sea.Hacelia tuberculata sp. nov. shows affinities withH. tyloplax (H. L. Clark, 1914) andLinckia gracilis sp. nov. is related toL. laevigata (Linnaeus). Contribution No. 1163 from the Institute of Oceanology, Academia Sinica.     

New genus and species of polyplacophora (Mollusca) from the East China Sea

The present paper dealing with two new species and one new genus of the Polyplacophora, is the first of a series of reports of systematic study on the deep sea mollusca collected by the research vesselsJINXING andKEXUE I of the Academia Sinica, Institute of Oceanology, during 1978 and 1981 from the continental margin to the bathyal zone of the East China Sea. Contribution No. 1647 from the Institute of Oceanology, Academia Sinica.     

DISTRIBUTIONS OF TOTAL, INORGANIC AND ORGANIC PHOSPHORUS IN THE SEDIMENTS NEAR THE HUANGHE RIVER ESTUARY

For some hundred surface sediment samples from five cores taken in two cruises near the Huanghe River Estuary, total phosphorus (TP (.inorganic phosphorus (IP (and organic phosphorus (OP)were determined.On the average, 527×10-6, 455×10~6 and 72×10-6 were found for TP,IP and OP for the surface sediments taken in the two cruises. The distribution of OP and IP was controlled by the sample particle size: OP content increased with the decreasing of the sample particle size, while the maximal value of IP was found in the silt fraction due to the existence of apatite in our samples. Vertical distributions reflected well the channel change of the Huanghe River. Results from the multiple regressions between the three forms of phosphorus and the percentages of different particle size agreed well with the analytical data.     

SOME NEW SPECIES OF NANNOPLANKTON IN JIAOZHOU BAY, SHANDONG, CHINA

Three new spades and a new variety of nannoplankton, Chrysochromulina papillata, Gaysochromulina chiton var. minuta, Paraphysomonas simplexocorbita and Paraphysomonas bisorbulina are reported in this paper. All were isolated from the preliminary culture samples of seawater collected from Jiaozhou Bay, Shandong, China. The three species occurred at Station 1(120° 14.56′ E, 36°4′N) in November 1984, the new variety at Station 2 (120° 16.35° E,36°4.5′N) in January, 1985. The morphological features, especially the structures of the scales of these new nannoplankton,. are described. The differences between the new species and the related ones are discussed; their movement and nutrition, and the temperature and salinity of their biotopes are also mentioned.     

Brachiopods from China seas

A collection of brachiopods by the Institute of Oceanology, Academia Sinica (Qingdao), contains eight species from seven genera. Six of the species have been recorded previously from China seas—Lingula adamsi, L. anatina, Discinisca stella, Pelagodiscus atlanticus, Campages mariae, Terebratalia coreanica. Two species (Terebratulina hataiana andFrenulina sanguinolenta) have been described from other parts of the Pacific area. The apparent absence of any endemic species is a noteworthy feature of Chinese in comparison with Japanese faunas.     

The influence of the sea-air heat exchange over the Kuroshio in the East China Sea in winter on the precipitation in the middle and lower reaches of Changjiang River in flood season

In this paper, the sea-air heat exchange over the Kuroshio in the East China Sea during the winters of 1954–1972 is computed and analysed. The results indicate that the year to year anomalies of sea-air heat exchange are obvious and the values are mainly controlled by the intensity of the cold air at the same time. The authors have found a close relationship between sea-air heat exchange and precipitation in the region of the Changjiang River during the early summer. Finally, the predicting indication for forecasting this precipitation around the middle and lower reaches of Changjiang River in June is given. Contribution No. 839 from the Institute of Oceanology, Academia Sinica. This paper was published in Chinese inOceanologia et Limnologia Sinica 14 (3): 256–262, 1983.     

A study of the family Dentaliidae (molusca) found in China

This paper describes 14 species of the family Dentaliidae collected from China by the Institute of Oceanology, Academia Sinica during the surveys on marine invertebrate animals and benthos in the past years. Of which 4 species are considered as new to science; 2 species are first recorded in China. The system adopted is mainly that of T. Habe (1964), but reference is also made to the works of W. Emerson (1962), P. Palmer (1974) and S. D. Chistikov. About 20 Chinese species of Dentaliidae have been described. We consider our study is still not complete and are sure that more species shall be found in further studies. Contribution No. 1456 from the Institute of Oceanology, Academia Sinica.     

Nutrients in seawater and interstitial water of the sediments in the Huanghe River estuarine area (November)

The investigation shows that the concentrations of nutrients are high in estuarine and coastal waters and low in offshore waters. The concentration of nitrate in estuaries is controlled through a physical mixing process and is also affected by biotic activity. The annual transport of total inorganic nitrogen and dissolved phosphate-phosphorus from the Huanghe River water to the sea is about 8.45 ×10⁴ and 1.45×10³ tons respectively. The distributions of inorganic nitrogen and silicate in interstitial water of surface sediments are similar to those in surface and bottom seawater. Their contents in interstitial water are 227–552 μmol/l (average375) for ammonia, 0.31–9.0 μmol/l (average 1.6) for nitrite, 0–41 μmol/l (average6.0) for nitrate, and 41–139 μmol/l (average 77) for silicate. The average concentrations of phosphate in the surveyed area are 0.64 μmol/l for seawater and 1.2 μmol/l for interstitial water. A cycle of phosphate in the estuary is also suggested in this paper. Contribution No. 1434 from Institute of Oceanology, Academia Sinica.     

Objective analysis and classification of the ten-day mean sea surface temperature variation in the East China Sea using singular value decomposition technique

The seasonal changes of the ten-day mean sea surface temperature in the East China Sea for the years 1953–1972 were studied using the singular value decomposition technique. Main results obtained are as follows: In the singular vector analysis of the ten-day mean sea surface temperature in the East China Sea, the first three singular vectors (or seasonal march functions) account for 97.76% of the variance of the data. The geographical distribution in the weightings (or spatial pattern functions) on these vectors shows a remarkable regional organization. An objective classification of the surface water mass in the East China Sea was based on the mode of its seasonal temperature changes. Contribution No. 801 from the Institute of Oceanology, Academia Sinica. An abstract of this paper was presented at an IAPSO Session of XVII IUGG held at Canberra, Australia, December 1979.     

Analysis of drift bottle and drift card experiments in Bohai Sea and Huanghai Sea (1975–80)

The results of drift bottle and drift card experiments in the Bohai Sea and Huanghai Sea obtained by researchers of the Institute of Oceanology, Academia Sinica from 1975–1980 are reported in this article. Of over 50,000 bottles and cards released, around 10,000 were recovered. The results gave some convincing evidences for the existence of the Huanghai Sea Coastal Current, the Huanghai Sea Warm Current, the cyclonic movement around the northern Huanghai Sea Cold Water Mass, the anticyclonic eddy in the area near the Shidao-Qingdao coast and the flow of some of the Huanghai Sea water to the Japan Sea and to the North Pacific off Tokyo. The results show that the drift bottles and cards are still useful for getting the flow pattern of enclosed and semi-enclosed seas. Contribution No. 1312, Institute of Oceanology, Academia Sinica, Qingdao.     

Cathodic protection of A1 hull ship

Heavy paint coating loss and localized corrosion of the Al alloy hull occur red after less than a year's service of a ship at sea. Examination and analysis showed that the Mg-basic sacrificial anodes originally used for cathodic protection were not suitable as the resulting −1.3V-plus (vs Ag/AgCl) protection potential for the hull was too negative for the purpose. A new protection system was designed in which the protection current densities were 15mA/m² for the hull and 350mA/m² for the propeller. The hull protection potential under actual condition at sea was between −920mV and −1100mV (Ag/AgCl) and the protection efficiency was over 90%. The protection areas were calculated by using the formulas A=1.8×LBP×D+BC×LB×B for the hull and A_s=2Aa_s for the propeller. Contribution No. 1640 from the Institute of Oceanology, Academia Sinica.     

Isolation of plasmid from the blue-green algaSpirulina platensis

CCC plasmid was isolated from an economically important blue-green alga —Spirulina platensis (1.7×10⁶ dalton from the S₆ strain and 1.2×10⁶ dalton from the F₃ strain) using a rapid method based on ultrasonic disruption of algal cells and alkaline removal of chromosomal DNA. The difference in the molecular weight of the CCC DNAs from the two strains differing in form suggests that plasmid may be related with the differentiation of algal form. This modified method, which does not use any lysozyme, is a quick and effective method of plasmid isolation, especially for filamentous blue-green algae. Contribution No. 79 of the Experimental Marine Biology Laboratory and 2153 of the Institute of Oceanology, Academia Sinica. This research was supported in part by The President of the Chinese Academy of Sciences Foundation.     

The insemination of goldfish (Carassium auratus) oocyte maturedin vitro

Full maturation of goldfish oocyte was inducedin vitro by 17 α-hydroxy-20β-dihydroprogesterone. The oocyte maturation involves GV migration to the periphery of the oocyte and germinal vesicle breakdown (GVBD). In the experiment, incubation duration for GVBD varied in different broods of oocytes. Generally, if the duration for GVBD was shorter than 6 h, oocytes would have a better chance to survive after maturation and insemination. The maturation of nucleus (GV) and cytoplasm are not synchronous. Cytoplasm maturation occurs several hs after GVBD. Oocytes inseminated 8–9 h after GVBD have the highest fertilizing and hatching rate. Fertilized ova maturedin vitro can develop to sexually mature adults capable of reproduction. Contribution No 1731 from the Institute of Oceanology, Academia Sinica.     

Effect of NH4-non the pigment content ofLaminaria japonica

Experiments withLaminaria japonica were conducted in Meidao Bay and Pier Bay, Qingdao, China, Nitrogen-starved plants were fertilized intermittently with 7.1 mM NH₄−N solution for 1 hour at 3 day intervals, after which chlorophyll a, fucoxanthin, chlorophyll c and β-carotene contents were analyzed. Photosynthetic and growth rates of plants and nitrogen content of seawater were determined. their contents of chlorophyll a, fucoxanthin, chlorophyll c and β-carotene were 1.65, 0.67, 0.33 and 0.06 mg.dm⁻² respectively, or 2.46, 2.03, 1.86 and 1.81 times those of the controls. The ratio of fucoxanthin to chlorophyll a in nitrogen-enriched plants was lower than that of the controls. Normal growth rate of the plants (2 cm·day⁻¹ in length) were reached when the chlorophyll a content exceeded 0.1 mg·g⁻¹ fresh wt., indicating that chlorophyll a content can serve as an indicator of normal growth. Experimental results show that seawater is regarded as fertile for the normal growth ofLaminaria if the total inorganic nitrogen (including ammonium salt, nitrate and nitrite) content in seawater is about 2 μm, and infertile if less than 1 μm, in which case fertilizer should be applied. Contribution No. 1703 from the Institute of Oceanology, Academia Sinica