Correlations between CPT and FVT Results for Busan Clay

In this study, CPT and FVT tests are carried out on young, normally consolidated clay (Busan clay, which is an unusually thick deposit) at several locations in the Nakdong River deltaic plane in Busan, Korea. Two correlation techniques are applied for estimating the cone factors for which the uncorrected and corrected field vane strengths are used as the reference strength. First, due to the scattered data over the entire depth, a direct correlation between the tested results (a method generally used in practice), is applied for each depositional environment (facies) that is detected from the CPT profiles and the geological analysis. Secondly, a special attempt is made to determine the cone factors based on the fact that the ratios of the field vane strength and the (q _t  ? σ _v0) and (u ₂ ? u ₀) values to the effective overburden pressure respectively, are approximately constant for each facies (the indirect correlation). It appears that the values obtained from the two techniques are almost identical. However, the direct correlation is sensitively affected by the fabric effects especially in some of the uppermost and lowermost layers of the clay and hence the arithmetic average values of each facies sometimes result in lower correlation coefficients. It is therefore inferred that the indirect correlation is more proficient for estimating the cone factors at each facies and can be used for practical applications. From the results of the indirect correlation, the cone factors of Busan clay are N _kt  = 7.13–13.06, N _kt,corr  = 9.45–17.07, N _Δu  = 6.10–9.88 and N _Δu,corr  = 8.41–12.24, the corrected values of which are considerably smaller or larger than the general ranges recommended by Lunne et al. (1997a). However, good correlations between N _kt and I _P , and between N _Δu (or N _kt ) and B _q , are not found for the clay.     

Photobleaching of chromophoric dissolved organic matter in natural waters: kinetics and modeling

The effects of monochromatic and polychromatic UV and visible (VIS) radiation on the optical properties (absorption and fluorescence) of chromophoric dissolved organic matter (CDOM) were examined for a Suwannee River fulvic acid (SRFA) standard and for water from the Delaware and Chesapeake Bays. The primary (direct) loss of absorption and fluorescence occurred at the irradiation wavelength(s), with smaller secondary (indirect) losses occurring outside the irradiation wavelength(s). The efficiency of both direct and indirect photobleaching decreased monotonically with increasing wavelength. Exposure to polychromatic light increased the CDOM absorption spectral slope (S), consistent with previous field measurements. An analysis of the monochromatic photobleaching kinetics argues that a model based on a simple superposition of multiple chromophores undergoing independent photobleaching cannot apply; this conclusion further implies that the absorption spectrum of CDOM cannot arise solely from a simple superposition of the spectra of numerous independent chromophores. The kinetics of CDOM absorption loss with the monochromatic irradiation were employed to create a simple, heuristic model of photobleaching. This model allowed us to examine the importance of the indirect photobleaching losses in determining the overall photobleaching rates as well as to model the photobleaching of natural waters under polychromatic light fields. Application of this model to natural waters closely predicted the change in the CDOM spectral shape caused by photodegradation. The time scale of this process was consistent with field observations acquired during the summertime for coastal waters in the Middle Atlantic Bight (MAB). The results indicate that the ratio of the photodegradation depth to the mixed layer depth is a key parameter controlling the rate of the photobleaching in surface waters.     

Determining confidence intervals in analyzing climatic series

In the context of the problem of global warming, the question of how much the observed increase in the mean air temperature over the last decades is likely due only to natural variability is studied. It is assumed that an observed temperature-data series of length N is a segment of a stationary random sequence with a spectrum known on an interval that does not include the lowest frequencies. The variance σ²(N) of the sample mean m* and the standard deviation σ(N), which determines the width of the confidence interval of m*, are calculated for different continuations of the spectrum to the low-frequency region. For a continuation of the spectrum that boundlessly increases, as does ω^−2α (0 < α < 1/2) when the frequency ω tends to zero (red-noise spectrum), it is shown that, the closer the parameter α is to 1/2, the more slowly σ(N) tends to zero at N → ∞. Using an empirical spectrum of a global mean annual temperature series as an example, it is shown that the standard deviation significantly depends on the form of the spectrum in the lowest frequency region. An attempt has been made to assess the measure of standard-deviation uncertainty due to a lack of exact information on the spectrum in the low-frequency region. Important series characteristics—the equivalent number of independent observations N _eq(N) and the time scale of correlation T ₁(N) which are determined through the variance σ²(N) of m*—also depend on the form of the spectrum in the low-frequency region. For the red-noise spectrum, N _eq(N) increases with an increase in N proportionally to N ^1–2α (but not proportionally to N as in the case of bounded spectrum); the correlation scale T ₁(N) is no longer constant (as in the case of bounded spectrum) and increases with an increase in N proportionally to N ^2α.     

光质对三角褐指藻光保护过程的影响

光能一方面驱动光合作用固定二氧化碳,另一方面当光能过剩就会对光合细胞产生氧化性损伤,甚至导致细胞死亡。硅藻等光合生物通过叶绿素荧光非光化学淬灭(NPQ)把过剩的光能转化为无害的热,达到保护细胞的目的。硅藻NPQ的诱导受硅甲藻黄素循环调节,并且与众多环境因子密切相关。作者通过对海洋环境的模拟,研究了不同光质下三角褐指藻(Phaeodactylum tricornutum)受到的影响测定并比较了红、绿、蓝3种单色光处理的三角褐指藻响应高光胁迫时的NPQ及硅甲藻黄素循环的差异。叶绿素荧光的结果显示,在相同的胁迫性强光和胁迫时间内,蓝光下处理的三角褐指藻形成的NPQ更高,而且胁迫阶段淬灭的荧光在低光阶段恢复的更快。色素测定的结果显示,3种单色光下处理的三角褐指藻的硅甲藻黄素循环的环脱氧比(DEPS)与对照组没有显著差异。实验结果表明,蓝光对三角褐指藻响应高光、形成NPQ至关重要。     

虹鳟(Oncorhynchus mykiss)选育群体主要体尺性状表型和遗传相关分析

以虹鳟(Oncorhynchus mykiss)优良品系选育群体作为试验群体,采用单性状动物模型估计头长、体长、体高、体厚、尾柄长、尾柄高、背吻距、背鳍基长等8个主要体尺性状的遗传力。结果显示,上述8个性状的遗传力在0.131—0.313之间,多为中等或偏低遗传力,其中背鳍基长遗传力最低,为0.131±0.039,体高遗传力最高,为0.313±0.086。采用皮尔逊相关法估计上述性状之间的表型相关,结果显示,上述性状间表型相关变化范围为0.016—0.815。采用两性状动物模型估计上述性状间的遗传相关,结果表明,上述性状间遗传相关变化范围为0.065—0.866。在本研究中,比较分析表型和遗传相关结果发现,虽然体厚与尾柄长的表型相关最低,仅为0.016,相关性不显著(P0.05),但是遗传相关为0.247,似然比检验(likelihood ratio test,LRT)统计分析达到显著水平(P0.05)。体厚与背鳍基长的表型相关为0.647,t检验达到显著水平(P0.05);但遗传相关仅为0.305,LRT统计分析未达到显著水平(P0.05)。上述结果说明,在该群体中各体尺性状的表型相关和遗传相关水平不完全相同,在设计育种方案时应综合考虑各个性状间的表型相关及遗传相关。     

海水叶绿素a现场测量仪研究

采用荧光测量方法研究成功的海水叶绿素a现场测量仪，是用于海上现场实时测量海洋中浮游植物体内叶绿素a含量的新型海洋仪器。本仪器采用低功耗、高强度、低重复频率、窄脉冲宽度的脉冲氙灯，为激发光源及相应的脉冲检测技术测量荧光信号。该仪器可在海表层至50m水深内任一日光强度下工作；测量海洋中浮游植物体内叶绿素a的浓度范围为1×10-6—1×10-9g／cm3；当仪器走航拖曳速度为2m／s时，其空间分辨度为1m，仪器具有海上现场测量实用价值。     

Evaluation of compression index of marine fine-grained soils by the use of index tests

Marine fine-grained soils are well known for their compressibility, which is typically measured and reported in terms of compression index, C_c. The difficulties associated with measuring C_c have resulted in growing research interest in statistics-based estimates (i.e., correlation equations). Although many empirical and semiempirical correlations exist for estimating C_c, most available correlations are based on either data from nonmarine soils or data collected from Japanese and Korean marine clays. Thus, there are few correlations for marine clays from other parts of the world. In the present study, two independent databases which contain a total of 1,000 data points from 170 different sites worldwide are used to build and validate statistically significant correlations for estimating compression index of marine soils. The results of this study suggest that (1) the proposed correlation equations provide quite good estimates of C_c for marine soils with different stress histories and sensitivities and (2) most of the existing models have unacceptable performance when they are applied to marine soils.     

Lack of association between color morphs of the Jellyfish Cassiopea andromeda and zooxanthella clade

Understanding the ecology and evolution of the cnidarian-algal symbiosis is of major scientific interest as it is sensitive to temperature and strong light and may therefore be susceptible to climate change. The stability of this mutualism is often mediated by host color pigments that influence photosynthetic activity in symbiotic dinoflagellates either by providing the photosystem with irradiance of suitable wavelength or by protecting it from much too much and potentially damaging light. Like scleractinian corals, the upside-down jellyfish, Cassiopea andromeda, relies heavily on the nutrients provided by its symbionts of the dinoflagellate genus Symbiodinium. It occurs in several conspicuously different color morphs and is found in habitats with high levels of irradiation. We tested whether the color morphs of Cassiopea were correlated with the Symbiodinium distribution in the host and whether host color was associated with different clades of Symbiodinium. We found that the presence of color pigment did not correlate with the distribution of Symbiodinium in the host. Symbiodinium was found in both the colored tentacles of the jellyfish and the colorless feeding tentacles. At least six different color morphs co-occurred in the very shallow waters of the Red Sea, but they all hosted a single Symbiodinium clade (clade A1). Therefore, no correlation of host color morph and Symbiodinium clade could be found. Photoaccumulative or photoprotective functions of host pigments, as proposed for some scleractinian corals, thus seem unlikely in the colored tentacles (vesicles) of the upside-down jellyfish Cassiopea andromeda.     

Modeling ocean primary production: Sensitivity to spectral resolution of attenuation and absorption of light

Modeling the vertical penetration of photosynthetically active radiation (PAR) through the ocean, and its utilization by phytoplankton, is fundamental to simulating marine primary production. The variation of attenuation and absorption of light with wavelength suggests that photosynthesis should be modeled at high spectral resolution, but this is computationally expensive. To model primary production in global 3d models, a balance between computer time and accuracy is necessary. We investigate the effects of varying the spectral resolution of the underwater light field and the photosynthetic efficiency of phytoplankton (α^*), on primary production using a 1d coupled ecosystem ocean turbulence model. The model is applied at three sites in the Atlantic Ocean (CIS (60°N), PAP (50°N) and ESTOC (30°N)) to include the effect of different meteorological forcing and parameter sets. We also investigate three different methods for modeling α^* – as a fixed constant, varying with both wavelength and chlorophyll concentration [Bricaud, A., Morel, A., Babin, M., Allali, K., Claustre, H., 1998. Variations of light absorption by suspended particles with chlorophyll a concentration in oceanic (case 1) waters. Analysis and implications for bio-optical models. J. Geophys. Res. 103, 31033–31044], and using a non-spectral parameterization [Anderson, T.R., 1993. A spectrally averaged model of light penetration and photosynthesis. Limnol. Oceanogr. 38, 1403–1419]. After selecting the appropriate ecosystem parameters for each of the three sites we vary the spectral resolution of light and α^* from 1 to 61 wavebands and study the results in conjunction with the three different α^*estimation methods. The results show modeled estimates of ocean primary productivity are highly sensitive to the degree of spectral resolution and α^*. For accurate simulations of primary production and chlorophyll distribution we recommend a spectral resolution of at least six wavebands if α^* is a function of wavelength and chlorophyll, and three wavebands if α^* is a fixed value.     

基于灰色系统西南大西洋阿根廷滑柔鱼资源丰度预测模型的构建

阿根廷滑柔鱼（Illex argentinus）是西南大西洋鱿钓渔业的主要作业鱼种,对资源丰度进行准确的预测可指导企业合理安排渔业生产。因此,本研究根据2000-2016年我国西南大西洋阿根廷滑柔鱼的生产数据,以单位捕捞努力量的渔获量（Catch per unit effort, CPUE）为阿根廷滑柔鱼资源丰度的指标,利用灰色绝对关联分析和灰色预测建模的方法（GM（0, N））,计算2001-2015年CPUE的时间序列值与产卵期（6-8月）产卵场海表面温度（Sea surface temperature, SST）时间序列值的灰色绝对关联度,选取产卵场海域中灰色绝对关联度大于0.90的海区SST建立资源丰度预测模型,并用2016年实际CPUE进行验证。灰色绝对关联分析表明,6-8月,30°~40°S,45°~60°W海域内存在若干海区的SST与次年对数CPUE时间序列呈现较强的关联度,可作为预报因子。GM（0, N）模型结果表明,以6-8月产卵场SST作为环境因子建立的模型4能较好地拟合出阿根廷滑柔鱼资源丰度变动趋势,与2016年真实值相比,相对误差为7%,该模型可较好地作为阿根廷滑柔鱼资源丰度的预测模型。相反,包含6月和7月SST的模型1效果优于不包含6月SST的模型2或不包含7月SST的模型3,拟合得到的2016年的数据与真实值相比,相对误差分别为128%和289%,这说明6月和7月是西南大西洋阿根廷滑柔鱼的主要产卵月份。     

Recovery of underwater visibility and structure by polarization analysis

Underwater imaging is important for scientific research and technology as well as for popular activities, yet it is plagued by poor visibility conditions. In this paper, we present a computer vision approach that removes degradation effects in underwater vision. We analyze the physical effects of visibility degradation. It is shown that the main degradation effects can be associated with partial polarization of light. Then, an algorithm is presented, which inverts the image formation process for recovering good visibility in images of scenes. The algorithm is based on a couple of images taken through a polarizer at different orientations. As a by-product, a distance map of the scene is also derived. In addition, this paper analyzes the noise sensitivity of the recovery. We successfully demonstrated our approach in experiments conducted in the sea. Great improvements of scene contrast and color correction were obtained, nearly doubling the underwater visibility range.     

大菱鲆(Scophthalmus maximus)幼鱼生长性状的遗传力及其相关性分析

以4种不同地理群体大菱鲆为亲本,采用巢式设计方法和人工采卵授精技术,按照1雄配2雌的原则,构建了28个父系半同胞家系和56个母系全同胞家系,分别测定了每个母系生长到6月龄的全同胞个体60个后代的体长、全长、体高、体重,应用数量遗传学原理,利用全同胞资料,采用方差、协方差分析的方法,研究了大菱鲆6月龄生长性状的遗传力及性状间的遗传相关和表型相关。对性状遗传力研究结果表明,雌性遗传方差组分均大于雄性遗传方差组分,雌性遗传方差组分存在显著的母性效应。基于父系半同胞组内相关法估计的遗传力是大菱鲆体长、全长、体高、体重遗传力的无偏估计值,估计的遗传力准确可靠,估计值分别为0．282、0．251、0．283、0．450,为中高等遗传力,显示对大菱鲆生长性状进行选择育种具有很大的潜力。对性状间相关性分析结果表明,依据父系半同胞遗传协方差组分和表型协方差分别估计体长．全长、体长一体高、体长．体重、全长。体高、全长一体重、体高一体重间的遗传相关和表型相关,遗传相关在0．888-0．985,表型相关在0．864—0．957,各性状间遗传相关和表型相关经t检验均达到极显著水平（P〈0．01）。     

Wave-current force on horizontal cylinder

For the calculation of wave-current force on horizontal cylinder a modified Morison's equation is used. A redefined Keulegan- Carpenter number KC2 is determined for the horizontal cylinder in wave-current co-existing field. The force coefficients are well related to the redefined KC2 number. As to the comparison with the force on vertical cylinder, the characteristics of force on horizontal cylinder are quite similar to those on vertical cylinder, but the force coefficients for horizontal cylinder are larger than those for vertical cylinder. It is proved by the authors' calculation that the results of monochromatic wave can be used directly for the determination of irregular wave-current force on horizontal cylinder in time domain.     

小黄鱼(Pseudosciaena polyactis)形态性状与体质量的相关性及通径分析

为了研究野生小黄鱼(Pseudosciaena polyactis)繁殖后代的形态性状对体质量的影响作用,以随机采集的729尾4.5月龄小黄鱼为研究对象,对每尾个体的体质量(g)和8个形态性状(cm):全长(X1)、体长(X2)、头长(X3)、躯干长(X4)、尾部长(X5)、尾柄长(X6)、尾柄高(X7)和体高(X8)进行准确测定,运用相关分析、多元回归分析和通径分析方法,分别计算性状间的相关系数、构建形态性状与体质量的多元回归方程,获得了通径系数和决定系数,进行了养殖小黄鱼的形态性状对体质量的影响研究。结果显示,8个形态性状与体质量的相关系数均达到极显著水平(P0.01);通径分析中,4个形态性状对体质量的通径系数达到极显著水平(P0.01),它们是影响体质量的重要指标,其中体长(通径系数:0.589)对体质量的直接影响最大。应用逐步多元回归分析建立了以体质量为因变量(Y),体长(X2)、躯干长(X4)、尾柄高(X7)和体高(X8)为自变量的回归方程:Y=–32.377+3.064X2–1.511X4+14.285X7+4.438X8。模型等式中形态性状与体质量的相关指数R2=0.927,说明所选性状是影响体质量的主要形态性状。以上分析结果为小黄鱼的选育测量指标的确定提供了理论依据。     

泥蚶(Tegillarca granosa)基因组SSR和EST-SSR的开发及比较研究

采用SSPHunter软件在泥蚶(Tegillarca granosa)磁珠富集文库和454转录组文库中搜索核心序列长度12bp以上的2—6核苷酸重复序列,根据富集文库所得基因组SSR侧翼序列设计47对引物,可成功扩增17对且均为多态性引物,多态率100%,平均等位基因数(Na)、观测杂合度(Ho)、期望杂合度(He)、多态性信息含量(PIC)分别为6.8、0.468、0.785、0.733。11344条EST序列中得到1683个SSR位点,检出率14.83%,平均每3.85kb出现1个位点。根据部分EST序列设计120对引物,62对可扩增出目的片段,其中29个位点具有多态性,多态率为46.77%,平均Na、Ho、He、PIC分别为4.2、0.372、0.554、0.500,均低于基因组SSR。双变量相关性分析的结果表明,46个多态性SSR位点的核心序列长度与其Na、Ho、He、PIC极显著正相关,Spearman相关系数分别为0.632、0.387、0.657、0.6640     

利用实时荧光定量PCR法测算红螯螯虾实体组织的细胞数量

螯虾是重要的水产养殖动物,也是研究甲壳动物疾病与免疫的一个良好模型.在研究不同组织的功能及受病原感染的影响时,需要了解组织内的细胞数量.但是,目前常用的细胞计数方法仅适用于单细胞悬液,无法用于实体组织.本研究以红螯螯虾( Cherax quadricarinatus)为对象,建立了基于基因拷贝数定量分析的实体组织细胞数...     

红鳍东方鲀(Takifugu rubripes)耐低温性状和生长性状遗传参数评估

采用人工控温的方式, 对构建的31个F₁红鳍东方鲀(Takifugu rubripes)全同胞家系开展低温胁迫实验, 获得耐低温性状低温累计存活时间(CDH), 基于混合线性模型分别开展耐低温性状和生长性状遗传参数评估,对每一性状是否需要考虑共同环境效应所构建的两种模型进行似然比检验。结果显示, 经似然比检验, 最终选用模型A和模型BF进行耐低温和生长性状遗传评估; 耐低温性状CDH遗传力为(0.27±0.08),属于中等遗传力; 体重BW遗传力为(0.36±0.13), 属于中等遗传力, 体长BL遗传力为(0.14±0.06), 属于低等遗传力, 经检验, 遗传力估计值均达到极显著水平(P<0.01)。CDH和体重、体长的遗传相关分别为(-0.40±0.22)和(-0.44±0.24), 表型相关分别为(-0.09±0.06)和(-0.16± 0.05), 均为负相关; 体重和体长之间的遗传相关为(0.92±0.05), 表型相关为(0.80±0.02), 呈正相关且结果极显著(P<0.01)。研究结果表明, 红鳍东方鲀的耐低温性状和生长性状都具有较好的改良潜力, 考虑到两性状间存在负遗传相关, 在开展耐低温选育时, 对首先不同性状进行品系选育, 然后利用品系间杂交培育出耐高温、生长快的新品种。该项研究首次完成了红鳍东方鲀耐低温性状的遗传参数评估, 为制订红鳍东方鲀耐低温选育育种规划提供了理论依据。     

Estimation of the <Emphasis Type="Italic">in situ</Emphasis> Ultraviolet-A absorption of seawater by a simple irradiance inversion model

An irradiance inversion model to estimate the in situ absorption coefficient of seawater has been developed for the Ultraviolet-A (UVA) wavelength domain. Input parameters are sun angle and the up-and downward planar irradiances measured for at least two depths. The present method does not require seawater to be sampled, and is a discrete wavelength method which returns the absorption coefficient at a given wavelength from the irradiances measured at that wavelength without assuming a spectral shape of any optical properties a priori. Comparison between the model results and spectrophotometric measurements shows that the model is practically useful when cloud cover in the atmosphere is ≤ 50%. According to the present method, measurements of the irradiances enable simultaneous observation of the in situ underwater UVA radiation level and the absorption capacity of bulk seawater using a radiometer.     

Phytoplankton pigment change as a photoadaptive response to light variation caused by tidal cycle in Ariake Bay,Japan

Underwater light environment and photosynthetic accessory pigments were investigated in Ariake Bay in order to understand how change of the pigments occurs in response to the tidal-induced changes in underwater light conditions. We hypothesize that phytoplankton increases photo-protective pigments and decreases light-harvesting pigments under higher light condition in the mixed layer caused by tidal cycle. Contribution rates of non-phytoplankton particles (a _nph (400–700)) for light attenuation coefficient (K _d ) was highest (32–85%), and those of phytoplankton particles (a _ph (400–700)), dissolved organic matter (a _g (400–700)) and water were 6–32, 6–21 and 5–23%, respectively. Mean K _d was higher during the spring tide (0.55 ± 0.23 m⁻¹) than the neap tide (0.44 ± 0.16 m⁻¹), and the K _d difference was caused by the substances resuspension due to the tidal current. In contrast, ratios of photo-protective pigments (diadinoxanthin and diatoxanthin) per chlorophyll a ((DD+DT)/Chl a) were higher during the neap tide (0.10 ± 0.03 mg mg-Chl a ⁻¹) than the spring tide (0.08 ± 0.03 mg mg-Chl a ⁻¹). And there was significant positive correlation between (DD+DT)/Chl a and mean relative PAR in the mixed layer ($ \overline {I_{mix} } $ \overline {I_{mix} } ). Moreover, there was significant negative correlation between ratios of light-harvesting pigments (fucoxanthin) per Chl a (Fuco/Chl a) and $ \overline {I_{mix} } $ \overline {I_{mix} } . These results suggested that phytoplankton in Ariake Bay increase photo-protective pigments and decrease light-harvesting pigments in the higher light condition of less turbid, shallower mixed layer during neap tide than spring tide.     

硬壳蛤形态性状与体质量、软体质量及肥满度的相关性和通径分析

为研究硬壳蛤形态性状对体质量(Y)、软体质量(Y1)及肥满度(K)的影响,测量硬壳蛤的壳长(X1)、壳宽(X2)、壳高(X3)、体质量(Y)和软体质量(Y1)等指标,并进行相关性分析、多元回归分析和通径分析。结果表明:体质量变异系数最高,壳宽与体质量相关系数最高(0.970)、壳长与软体质量相关系数最高(0.946)、壳长与肥满度相关系数最高(0.203)。壳宽对体质量直接作用最大(0.145)、壳长对软体质量直接作用最大(1.113)、壳长对肥满度直接作用最大(1.752)。形态性状与体质量、软体质量及肥满度的多元回归方程分别为Y=–46.359+0.288X1+1.723X2+0.640X3,R2=0.949;Y1=–6.607+0.301X1+0.157X2–0.161X3, R2=0.901; K=3.159+0.173X1+0.008X2–0.179X3, R2=0.072。壳宽是影响硬壳蛤体质量的主要因素,壳长是影响硬壳蛤软体质量的主要因素,各性状与肥满度回归关系不显著,个体间肥满度差异大。研究结果可为硬壳蛤选育及苗种繁育提供参考。