海鱼AChE监测海水有机磷农药的可行性研究

有机磷农药(OPs)对乙酰胆碱酯酶(AChE EC 3.1.1.7)具有专一性抑制作用,因此AChE常作为淡水和土壤OPs污染的特异性标识物(biomarker).本文在海水背景下,通过测定两种有机磷农药(马拉硫磷和甲基对硫磷)对海洋鱼类-鲈鱼[Lateolabrax japonicus(cuvier)]脑组织AChE的抑制效应,分析了海鱼AChE监测海水OPs的可行性.结果显示,抑制时间为20min时,抑制程度达到相对稳定;在低浓度OPs抑制下,纯酶液比粗酶液表现出更好的敏感性,对马拉硫磷和甲基对硫磷的灵敏度分别达到0.28μg*L-1和0.2μg*L-1;在一定浓度范围内,AChE活力对OPs具有显著的线性响应.研究表明,利用鲈鱼AChE指示和监测海水OPs的微污染是完全可行的.     

In vitro and in vivo inhibition of acetylcholinesterase and carboxylesterase by metals in zebrafish (Danio rerio)

Metals are natural components in ecosystems; however, if these elements are in excess, they can have adverse effects on living organisms. This study analyzes the interference of copper, lead, iron and cadmium in acetylcholinesterase (AChE) and carboxylesterase (CbE) activities in zebrafish. AChE was significantly inhibited in vitro by copper, iron, lead and cadmium at higher concentrations (10 and 20 mmol/L), whereas CbE was inhibited only at a concentration of 20 mmol/L. In vivo, only lead and cadmium were able to cause AChE inhibition at higher concentrations, while iron didn't cause any changes, and copper promoted an increase in AChE activity at a concentration of 0.06 mg/L. CbE activity did not change at any of the times (two and seven days) and concentrations tested, except in the case of copper exposure, which resulted in a decrease in CbE activity. Indeed, iodoacetamide treatment didn't changed AChE neither CbE activities, results which indicate that the metal inhibiting effect is probably not due to its biding to thiol groups close the active site of the enzyme. This outcome reveals that metals are important esterase inhibitors in zebrafish, and should be considered in environmental monitoring studies that use esterase inhibition as exposure biomarkers of organophosphate and carbamate pesticides.     

蔬菜农药残留GC-MS分析方法改进与基质效应探讨

改进了国家标准GB/T5009.20-2003和农业部标准NY/T761-2004中的2种样品前处理方法,适用于GC-MS多农药残留检测,并探讨了基质效应的影响。使用GC-MS-SIM检测,做到了快速准确地检测多种农药残留、排除假阳性;采用基质标准结合单点校正进行定量分析,解决了保留时间的漂移,基质增强效应和个别有机磷农药拖尾的问题。建议使用农业部标准的改进方法进行多种农药的检测,而在甲胺磷或乙酰甲胺磷的单项检测中使用国标改进方法。采用2次脱水的国标改进方法,加标回收率在70%~105%;采用丙酮 正己烷混合溶剂溶样的农业部标准改进方法,加标回收率在90%~105%(甲胺磷和乙酰甲胺磷在65%左右),2种方法的相对标准偏差均在15%以内。     

有机磷农药对海洋动物乙酰胆碱酯酶(AChE)的毒性效应研究进展

近几十年来，农业和海水养殖业所使用的有机磷农药(OPs)造成沿岸海水的严重污染，威胁到海洋动物的生存。有机磷农药的海洋生态毒理学研究已经成为海洋环境科学中的热门领域之一。作为动物神经传导过程中的关键酶，乙酰胆碱酯酶(AChE)能够被有机磷农药专一性抑制，因此，有机磷农药对海洋动物AChE的影响受到更多的关注。本文以近年来该领域的研究成果为基础，综述了有机磷农药对海洋动物乙酰胆碱酯酶的毒性效应。     

Characterization of cholinesterase activity from different tissues of Nile tilapia (Oreochromis niloticus)

Cholinesterases (ChE) from brain, muscle and liver in Nile tilapia (Oreochromis niloticus) were characterized using three substrates: acetylthiocholine iodide, propionylthiocholine iodide, and butyrylthiocholine iodide. Eserine was used as a total ChE inhibitor; BW284c51 and iso-OMPA were used as selective inhibitors for acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), respectively. The results indicate that AChE is the enzyme present in brain, whereas in both liver and muscle, the presence of atypical ChEs are suggested. These findings indicate that characterization of ChE is necessary prior to use in monitoring programs.     

Flounder health status in the Seine Bay. A multibiomarker study.

The Seine Bay is used as a pilot area to assess the usefulness of monitoring programmes using a suite of biological measurements. These biomarkers included ethoxyresorfin-O-deethylase (EROD) and acetylcholinesterase (AChE) activities, multixenobiotic resistance (MXR) protein expression level assessment and gonad histopathology. Samples of European flounder collected in three sites close to the Seine Estuary in late September 1998 showed that 8% of the males were intersex, i.e. had gonads with both male and female tissues. Another 10% of individuals, identified as male by morphological observation during sampling, showed only female tissues on histological sections. These dramatic changes were associated with different patterns of EROD activity, MXR expression or AChE activity inhibition that might reflect shorter time effects of xenobiotics and constitute a starting point to integrate biological responses for the assessment of the health status of flounder in the Seine Bay.     

基于SAR极化比和纹理特征的海面溢油识别方法

针对海洋表面SAR影像的特点,采用基于灰度共生矩阵的纹理特征方法是提取海面溢油信息的常用方法,但实际海洋表面复杂的信息使得SAR图像上产生类似溢油现象的暗斑区域,这导致在利用纹理特征方法提取溢油信息时存在虚警率,降低了溢油信息的提取精度。基于RADARSAT-2 SAR四极化影像,本文提出基于SAR极化比影像的纹理特征识别方法对海面油膜进行识别提取。结果显示,基于SAR极化比影像的纹理特征识别方法可以有效且准确地提取海面溢油信息,相比于VV极化影像的纹理特征识别方法,溢油监测过程中的虚警率降低了17.96%,溢油监测总体精度达到96.83%。     

无人机海上溢油跟踪监测系统设计及仿真

海上环境变化多端,造成溢油的漂移和扩散会出现不可预测的情况,精确、实时地监测海上溢油是现今亟待解决的问题.无人机以其部署快、成本低、环境适应性强的优势在海上溢油监测领域得到重视,但单架无人机监测能力弱,而多架无人机监测的准确性仍需提高.为此,本文提出一种无人机群海面溢油自动导航跟踪监测的架构和方法,根据海上溢油浓度的变化进行路径规划.该方法包括建立溢油模型和设计无人机跟踪控制系统.溢油模型主要描述海上溢油时空变化的形态复杂性;控制器可控制无人机追踪和监测溢油漂移及扩散的情况.同时,将无人机跟踪控制系统与人工势场法相结合,避免无人机相撞.最后,进行数值仿真,结果表明该跟踪系统与溢油的重合率达到70％～80％,验证了该方法的可行性.未来,该系统可广泛应用于无人机群对不同环境现象和灾害的跟踪监测.     

Bioaccumulation and biomarkers in the sea star Asterias rubens (Echinodermata: Asteroidea): a North Sea field study

During two North Sea field trips in March 1995 and September 1996 sea stars, Asterias rubens, were collected at various stations along pollution gradients in order to study the relation between biochemical markers and levels of accumulated contaminants. Biomarkers measured were: cytochrome P450 level, benzo[a]pyrene hydroxylase (BPH) activity, acetylcholinesterase (AChE) activity and DNA integrity. Accumulation levels of heavy metals, polychlorinated biphenyls, organochlorine pesticides and polycyclic aromatic hydrocarbons (PAHs) in the pyloric caeca of sea stars indicate different pollution gradients, influenced by rivers in The Netherlands, UK and Germany. For some contaminants, especially PAHs, relatively high levels were found in the central part of the North Sea (Dogger Bank). On the basis of multivariate statistics, stations near the mouth of the Elbe and the Rhine/Meuse were shown to have different patterns of biomarker responses. Sea stars from stations in coastal zones showed relatively high levels of cytochrome P450 and 'P418', another haemoprotein that is present in most marine invertebrates. The station nearest to the Elbe Estuary showed the lowest BPH and AChE activity. DNA integrity was lower especially in stations near the Dutch coast and in a station near the Tees/Tyne estuaries. Using these biomarkers as early warning signals of exposure and/or adverse effects, this type of monitoring can be used also in the future to study the spatial and temporal trends in the quality of coastal waters.     

Satellite Monitoring of the Northern Territories Disturbed by Oil Production

The results of satellite monitoring of the state of northern territories disturbed by oil production are presented by the example of the Usinsk oil field in the Komi Republic. The sets of vegetation indices formed by the results of processing long-term series of multispectral satellite images for the period from 1988 to 2014 are analyzed. They are used to assess long-term environmental changes, to reveal the most disturbed zones, and to estimate the dynamics of changes in the vegetation cover area caused by the extraction and transportation of hydrocarbons.     

应用极化合成孔径雷达检测海上溢油研究进展

海上溢油给海洋生态环境带来严重的影响,快速准确地探测溢油对于防灾减灾具有重要的意义。利用卫星遥感探测溢油已成为目前主要的检测手段,大多采用合成孔径雷达(SAR)数据,运用图像处理的方法,开展了多种溢油提取算法的研究,取得了较好的结果,但由于海洋的类溢油现象存在,造成提取信息的精度达不到要求。近年来,国内外运用极化SAR数据开展溢油信息提取研究,从极化分解与相位差等角度对溢油特性分析,能有效地区分一些类溢油现象,得到了较理想的结果。分析了应用SAR数据开展溢油信息提取的研究状况,总结了溢油极化SAR探测的研究,指出了目前研究中存在的不足,并提出了今后溢油极化SAR遥感监测的方向。     

1979-2010年我国大陆海岸潟湖变迁的多时相遥感分析

潟湖是重要的海岸湿地类型,具有丰富的自然资源和独特的生态功能。近几十年来,我国的海岸潟湖遭到了极大破坏,因此,开展我国海岸潟湖遥感监测与分析具有重要意义。本文根据1979年、1990年、2000年和2010年的4期共102景多时相遥感影像数据,利用遥感和地理信息系统等技术方法对我国大陆海岸潟湖进行了遥感监测,首次调查统计了我国大陆海岸潟湖的名称、数量、分布、岸线长度和面积信息,并对近31年来我国大陆海岸潟湖的变迁状况进行了分析。结果表明,1979年我国大陆海岸潟湖共计251个,近31年,共消失19个潟湖,其中辽宁省消失15个,广东省消失3个,广西壮族自治区消失1个;1979年我国大陆海岸潟湖岸线总长度为2 692.26km,总面积为1 092.805 5km2,至2010年我国大陆海岸潟湖岸线总长度共减少337.80km,潟湖总面积共减少204.103km2;31年间,我国大陆海岸潟湖的岸线长度和潟湖面积总体均处在持续减少的状态,各省(自治区)也基本符合该规律;1979-2010年间,我国大陆海岸潟湖大部分处在老年期,死亡期潟湖消失19个。     

基于合成孔径雷达回波信号的海洋溢油监测方法研究

海洋油污染是各类海洋污染中最常见、分布面积最广且危害程度最大的污染之一。近年来,海洋特别是近海人类活动频繁,且随着海上运输和石油加工业的发展,油田井喷、钻井平台爆炸、船舶碰撞等所造成的溢油事故增多,因而,监测海洋溢油具有重要的经济和社会现实意义。研究采用MatLAB工具,通过图像预处理(图像校正和增强)、特征提取和神经网络识别等方法,对合成孔径雷达(SAR)海洋溢油图像进行处理,最终期望实现半自动区分SAR图像上各类目标,并进行多种神经网络方法效果比较。研究首先对SAR海洋溢油图像进行初步人工识别;然后进行图像预处理(几何校正、滤波处理等)和基于灰度共生矩阵的特征值计算;最后,借助神经网络方法对溢油区域和疑似溢油区域进行分类,输出分类处理后的图像。通过输出图像分析发现,神经网络能对SAR海洋溢油图像中溢油、海水、土地3类目标进行明确分类,且RBF神经网络模型精度高于BP神经网络。本文提出的半自动分类方法不仅能提高SAR图像处理效率,将分类目标扩充有溢油和非溢油扩充到溢油、海水、土地3类,提高图像处理的全面性,同时通过比较RBF和BP神经网络在SAR溢油图像分类上的具体优劣,有着较好实际意义。     

原油WSF对三种海洋鱼类肝微粒体EROD活性的诱导和恢复的比较

研究了实验室条件下原油水溶性组分(WSF)暴露对黑鲷、黄鳍鲷和褐菖鲉肝微粒体EROD活性的剂量-效应,时间-效应和恢复过程。实验结果表明,在剂量诱导实验中,褐菖鲉肝EROD活性在原油WSF浓度为50μg/dm3时呈现生物统计学上的显著差异,而黑鲷和黄鳍鲷肝EROD活性在75μg/dm3时才呈现生物统计学上的显著差异;褐菖鲉肝EROD活性诱导倍数最高,但黑鲷的诱导浓度范围较广。在时间诱导实验中,在40μg/dm3原油WSF暴露下黄鳍鲷肝EROD活性在2 d时首先呈现显著差异;三种鱼肝EROD活性均在第4天达到最高,并呈现显著性变化,此后随着暴露时间的延长而逐渐下降并接近对照组水平。在恢复实验中三种鱼肝EROD活性下降并恢复到对照组水平。研究结果表明:对于石油污染物,黑鲷、黄鳍鲷和褐菖鲉肝EROD活性都可以作为污染生化效应监测指标,然而就三种鱼类比较而言,褐菖鲉最敏感,更适合于作为石油类污染及其生化效应,尤其是低剂量效应的监测生物。     

南沙珊瑚礁生态系中元素的垂直转移途径

于１９９３年和１９９４年用沉积物捕捉器采集南沙永暑礁和渚碧礁内的沉降颗粒物,用中子活化法分析元素的含量并计算通量,通过对生源要素的统计相关分析,垂直沉降颗粒物中的元素人,非生源要素在泻湖中的停留时间的探讨,研究了南沙珊瑚礁生态系中元素的垂直转移途径,结果表明,在南沙珊胡礁泻湖中元素的停留时间较海水中短,元素的循环速率高,且尤以与生物作用有关的元素为甚;颗粒物中非生源要素的垂直转移主要是通过生物过程     

渤海海域多源遥感溢油风险评价与长序监测

渤海海域溢油事件频发,所以进行海洋溢油风险时空特征分析,对于开展卫星遥感溢油精确监测具有重要意义.作者采用多源高分辨率卫星遥感数据,提取渤海海域船舶及石油平台两类主要溢油风险源分布状况,结合2015—2020年间渤海海域海上溢油卫星遥感监测结果,分析多种传感器下不同类型溢油的成像特征,通过溢油风险源核密度分析方法,获得...     

Limiting similarity, niche filtering and functional diversity in coastal lagoon fish communities

The fundamental idea behind the study of biodiversity patterns is the presumed connection between the shape of species assemblages and the functional ways in which they are organized, this functional organization referring to how species are related to one another, as competitors or members of a web of interactions and to how species are facing similar environmental constraints. Amongst the different facets of biodiversity, functional diversity is certainly a key for ecosystem processes in coastal areas. However, surprisingly, patterns of functional diversity have received little attention until now. After presenting a common framework linking functional diversity patterns to species coexistence theories, the aim of our study was twofold: (1) to seek assembly rules in brackish lagoon fish communities drove by functional traits. To this aim we used null models to examine the influence of two opposing forces acting on community structure: interspecific competition that might prevent the coexistence of the most similar species, and environmental filters that might result in the most similar species to coexist. (2) To seek relationships between fish functional diversity and environmental gradients, if any. Fish sampling was carried out in two coastal lagoons where stations differ considerably in terms of physicochemical parameters. Using morphological functional traits, functional diversity of fish communities was estimated using two recently published indices as well as a new proposed index. Firstly our study was not able to demonstrate a limitation of similarity in coexisting lagoon fishes due to interspecific competition. Conversely our results support the niche filtering hypothesis preventing species too dissimilar from one another to co-occur at the same station. Secondly, salinity was positively related to the functional diversity of fishes in both lagoons suggesting that within species assemblages near the channel species are less redundant than at stations far from the channel where species tend to be functionally similar (benthic and eating zooplankton).     

Assessment of pesticide contamination of groundwater in New Zealand

Groundwater contamination by pesticides may be affected by the amounts of pesticide used, the mobility and degradation characteristics of the pesticides, and the vulnerability of an area to pollution. An index of potential pollution was developed based on these factors, with the groundwater vulnerability being measured using the DRASTIC ranking method. Seventeen regions were selected from throughout New Zealand where significant pesticides have been applied and there is an underlying groundwater system. They were ranked according to this index of potential pollution. The sensitivity of the ranking to various formulations and weightings of the index was examined. Three regions (Poverty Bay, Te Puke, and Motueka) were generally highly ranked for a wide range of index formulations. Six of the higher‐ranked regions were selected for sampling in the second phase of the study.     

Brain acetylcholinesterase as a marine pesticide biomarker using Brazilian fishes

Brain acetylcholinesterase (AChE) of some fishes from the coast of Rio de Janeiro State was studied as a possible pesticide biomarker in marine environmental monitoring. AChE specific activity in brain varied from 145 to 530 U/g of proteins and the Michaelis-Menten constant (K(M)) for acetylthiocholine varied from 104 to 291 microM among the 20 species studied. The enzyme sensitivity to methyl paraoxon, evaluated by the inhibition kinetic constants, shows that some species (Paralonchurus brasiliensis and Genidens genidens) are more sensitive (IC50-30 min=455 and 468 nM, respectively). The less sensitive Merluccius hubbsi and Percophis brasiliensis (IC50-30 min=3339 and 3259 nM, respectively) belong to the super-order Paracanthopterygii, which includes the more ancient species. On the other hand, more susceptible species belong to the super-order Acanthopterygii, which includes more recent species. These results suggest a possible evolutionary linkage for AChE sensitivity to methyl paraoxon. The application of inhibition kinetic constants for fish brain AChE in phylogenetic studies is still being investigated. The results have shown that a fish sentinel species should have the highest brain AChE level among the more sensitive ones.