ESTIMATION OF SOUNDING ABILITY OF A BRILLOUIN LIDAR IN THE EAST CHINA SEA

Vertical profiles of sound speed in the sea can be measured by using laser excited Brillouin scattering. In this paper the dependence of the accuracy of sound speed measurement on the accuracy of the Brillouin shift measurement is analyzed. We calculated the maximum detecting depths of sound speed to an accuracy of 1 m/s by lidar with different laser pulse energy, platform altitude, telescope aperture and lidar effective attenuation coefficient. The estimation of sounding ability in the East China Sea is made in some stations. These data can be used in the design of Brillouin Lidar for the China Sea.     

The isolation of prophyra-334 from marine algae and its UV-absorption behavior

1 INTRODUCTIONSolar ultraviolet radiation on the earth's surfaceincreases with the depletion of stratospheric ozone(Crutzen, 1992; Kerr and McElroy, 1993; Mad-ronich et al., 1998). Ultraviolet radiation is a highlyactive component of solar spectrum. The excessiveUV radiation has deleterious effects on all plant,animal and microbial groups exposed to solar radia-tion, including the destruction of DNA, proteins andother molecules (Harm, 1980), inhibition of photo-synthesis and growth (W…     

A research on statistical retrieval algorithms and spectral characteristics of the total absorption coefficients in the Yellow Sea and the East China Sea

1 INTRODUCTION The Yellow Sea and the East China Sea are typical coastal Case-II waters with high sediment load and complicate optical properties. The total absorption coefficient of sea water is one of the main parameters influencing the calculation of o…     

Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically

The influence of alkali on astaxanthin and the optimal working wave length for measurement of astaxanthin from Haematococcus crude extract were investigated, and a spectrophotometric method for precise quantification of the astaxanthin based on the method of Boussiba et al. was established. According to Boussiba's method, alkali treatment destroys chlorophyll. However, we found that: 1) carotenoid content declined for about 25% in Haematococcus fresh cysts and up to 30% in dry powder of Haematococcus broken cysts after alkali treatment; and 2) dimethyl sulfoxide (DMSO)-extracted chlorophyll of green Haematococcus bares little absorption at 520-550 nm. Interestingly, a good linear relationship existed between absorbance at 530 nm and astaxanthin content, while an unknown interference at 540-550 nm was detected in our study. Therefore, with 530 nm as working wavelength, the alkali treatment to destroy chlorophyll was not necessary and the influence of chlorophyll, other carotenoids, and the unknown interference could be avoided. The astaxanthin contents of two samples were measured at 492 nm and 530 nm; the measured values at 530 nm were 2.617 g/100 g and 1.811 g/100 g. When compared with the measured values at 492 nm, the measured values at 530 nm decreased by 6.93% and 11.96%, respectively. The measured values at 530 nm are closer to the true astaxanthin contents in the samples. The data show that 530 nm is the most suitable wave length for spectrophotometric determination to the astaxanthin in Haematococcus crude extract.     

Researches on the stability of porphyra-334 solution and its influence factors

1　Introduction　Ultravioletradiationisahighlyactivecomponentofsolarspectrum .Ultravioletradiationhasdetrimentaleffectsonlivingthingsexposedtosunlight ,includingthedestructionofDNA ,proteinsandothermolecules(Harm ,1980 ) ,inhibitionofphotosynthesisandgrow…     

Effect of algae and water on water color shift

This study showed that the combined effect of absorption of planktonic algae and water on water color shift can be simulated approximately by the exponential function: Log(E _100cm ^W +E _100cm ^Xch1 )=0.002λ−2.5 whereE _{100 cm} ^W ,E _100cm ^Xchl are, respectively, extinction coefficients of seawater and chlorophyll—a (concentration is equal toX mg/m³), and λ (nm) is wavelength. This empirical regression equation is very useful for forecasting the relation between water color and biomass in water not affected by terrigenous material. The main factor affecting water color shift in the ocean should be the absorption of blue light by planktonic algae.     

The effect of pH on both spectral types of R-phycoerythrin

There are two spectral types of R-phycoerythrin (RPE) in red algae. One is the triple peak type RPE with absorption maxima at 498,540 and 565 nm. The other is the double peak type RPE with absorption maxima at 498 and 565 nm, and an absorption shoulder at 540 nm. The former is present only in higher classes of the more advanced Florideophyceae, the latter occurs chiefly in the more primitive Bangiophyceae. When these two types of RPE are treated with buffer solutions with a broad pH range from 2.0 to 11.0, their absorption and fluorescence spectra remain unchanged within the range adjacent to neutral pH. At pH values higher than 9.6, the absorption and fluorescence spectra of triple peak type RPE still remain stable, but the absorption spectra of double peak type RPE produce gradually a protrusion similar to an absorption peak at about 540 nm. This phennomenon shows that there are certain differences in structure between these two types of RPE. Both types of RPE are naturally occurring products showing systematic developmental meaning in red algae. Contribution No. 1333 from the Institute of Oceanology, Academic Sinica.     

Comparative studies on spectral properties of R-phycoerythrin from the red seaweeds from Qingdao

The present paper reports on comparative studies of R-phycoerythrin (R-PE) from 30 species of the red seaweeds in Bangiophyceae and in Florideophyceae from Qingdao. The measured absorption spectra show that R-PE from these red seaweeds have two spectral types, namely type I having two absorption peaks and type II having three absorption peaks. 9 specise of a lower red seaweed belonging to Bangiophyceae are all type I R-PE. 3 species of a higher red seaweed belonging to Florideophyceae are also type I R-PE. The remaining 18 species are all type II R-PE. According to the distribution of two types of R-PE in red algae, an evolutionary trend from type I R-PE to type II R-PE, can be seen, but this evolutionary process is more complicated and without a definite line of demarcation between the two spectral types of R-PE. The occurrence of two types of R-PE in red algae is of taxonomic significance. Project supported by the Science Fund of the Chinese Academy of Sciences. Contribution No. 1357 from the Institute of Oceanology, Academia Sinica.     

Retrieval of chlorophyll a and suspended solid concentrations by hyperspectral remote sensing in Taihu Lake, China

Chlorophyll α（ch1-α） and suspended solid concentrations are two frequently used water quality parameters for monitoring a lake. Traditional measurement of ch1-α and suspended solids, requiring laborious laboratory work, which is often expensive and time consuming. Hyperspectral remote-sensing measurement provides a fast and easy tool for estimating water trophic status. In situ hyperspectral data on March 7-8, July 6-7, September 20 and December 7-8, 2004 and the corresponding water chemical data were used to regress the algorithm of water quality parameters. Results showed that the peak of water leaving radiance around 700 nm （R700） varied proportionally with ch1-α concentration, and moved to infrared when algal bloom occurred. The reflectance ratio of R702/R685 was well correlated with ch1-α when water surface in no algal bloom case and the correlation coefficient was better if absorption of phycocyanin was considered. The reflectance ratio R620/R531 was highly correlated to the concentration of suspended solids. The relationship between suspended solids and other band groups were also compared. Secchi disk depth could be calculated by non-linear correlation with suspended solids concentration.     

ISOLATION, PURIFICATION AND CHARACTERIZATION OF THE HYDROGEN EVOLUTION PROMOTING FACTOR OF HYDROGENASE OF SPIRULINA PLATENSIS

A component (s-factor) with obvious promoting effect on hydrogen evolution of hydrogenase has beenisolated and extracted from a Cell=free preparation of Spirulina platensis.The effect of the s-factor in the re-action system is similar to that of Na_2S_2O_4, but is coupled with ligh. The s-factor has the maximumabsorption peak at 620 nm in the oxidized state, at 590 nm in the reduced state. The partially purifieds-factor showed two bands by SDS-PAGE and is distinctly different from phycocyanin,which has nochange of oxidized state and reduced state absorption spectra, and also has no promoting effect onhydrogenase of Spirulina platensis under the light.     

Measurement and feature analysis of absorption spectra of four algal species

Two methods for particulate pigments(i.e.,quantitative filter technique,QFT,and in vivo measurement,InVivo,respectively)and two methods for dissolved pigments(i.e.,Acetone Extracts,AceEx,and high-performance liquid chromatography,HPLC,respectively) were used to obtain the optical absorption coefficient spectra for cultures of four typical algal species.Through normalization and analysis of the spectra,it is shown that(1) the four methods are able to measure optical absorption spectra of particulate and/or dissolved pigments;(2)that the optical absorption spectra of particulate and dissolved pigments were consistent in terms of the peak position in the blue wavelength,and the difference of the peak position in the near infrared wavelength was ~10nm between each other;and(3)that the leveling effect of the absorption spectra of particulate pigments was significant.These four methods can all effectively measure the absorption coefficients of phytoplankton pigments,while each one has its unique advantages in different applications.Therefore,appropriate method should be carefully selected for various application due to their intrinsic difference.     

Isolation,purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis

A component (s-factor) with obvious promoting effect on hydrogen evolution of hydrogenase has been isolated and extracted from a cell-free preparation ofSpirulina platensis. The effect of the s-factor in the reaction system is similar to that of Na₂S₂O₄, but is coupled with light. The s-factor has the maximum absorption peak at 620 nm in the oxidized state, at 590 nm in the reduced state. The partially purified s-factor showed two bands by SDS-PAGE and is distinctly different from phycocyanin, which has no change of oxidized state and reduced state absorption spectra, and also has no promoting effect on hydrogenase ofSpirulina platensis under the light. The research supported by the NSFC.     

铁路用地地籍管理技术方法及相关问题研究

随着地籍管理科技发展,铁路用地地籍管理在引入国家大地统一坐标系的测量技术手段的同时,为满足铁路用地线状分布特点和日常管理的需要,仍然保留了独立坐标系统。而铁路运营设备的不断更新和技术装备的不断提高,铁路线路设备发生了较多拨移、变线或改道,作为确认铁路地界线位置的基准线,铁路线路的变化,对快速准确地查找铁路地界线产生了直接影响。为此,针对铁路用地参照坐标的变化情况及调整方法进行了深入调查研究,提出了可行性建议。     

一种适用于多制式移动通信的双阻双频段天线

为了满足现代多制式移动通信频段的需求,设计一种可覆盖大部分移动通信频段的超宽带天线。同时为了抑制与其他通信设备的干扰,在天线馈线旁加载两个U型谐振环用来获得双阻带特性。天线总尺寸为200mm×150 mm×3 mm。通过调整U型环可以获得不同的阻带特性。设计结果表明:天线工作在750~1150MHz和1650~2450MHz。该天线成本低、易集成,同时具有全向辐射特性,适用于现代移动通信需要。     

Subunit composition and chromophore content of R-phycoerythrin fromPorphyra haitanensis

R-phycoerythrin fromPorphyra haitanensis exists in two aggregation states with different molecular weights. A more highly aggregated form, RPE I, was chromatographed on Bio-Rex 70 column with urea solution (pH 3.0) as eluent, and the molecular weights of the 3 subunits (α, β, γ) obtained were determined on SDS-PAGE at 18000, 19200 and 30000, respectively. α subunit carried two phycoerythrobilin (PEB); β subunit, three PEB and one phycourobilin (PUB); γ subunit, one PEB and three PUB chromophores. The molar ratio of α, β, and γ subunits of RPE I was 6: 6: 1, and their subunit composition was confired to be (αβ)₆γ on account of the molecular weight of RPE I, 232000. A lower aggregated form, RPE II, contained α and β subunits similar to those of RPE I, but its subunit composition was the (αβ) monomer of RPE. Contribution No. 2108 from the Institute of Oceanology, Chinese Academy of Sciences     

Removal of phenol by Isochrysis galbana in seawater under varying temperature and light intensity

Phenol is a common industrial chemical produced and transported worldwide largely. Therefore, accidental spillage of phenol in the ocean causes an increasing concern. Microalgae are promising to remove phenol from marine waters. However, temperature and light intensity are two main factors that markedly influence biodegradation in marine environments. In this study, a marine golden alga Isochrysis galbana is selected to research the removal of phenol under different temperatures (10–30°C) and light intensities (0–240 µmol/(m2·s)). The results show that the most suitable temperature and light intensity for phenol removal are 20°C and 180 µmol/(m2·s), respectively, and 100 mg/L of phenol can be completely removed by microalga in 24 h at these conditions. I. galbana can also remove phenol under dark and low-temperature conditions. The removal of phenol by I. galbana at diverse temperatures and light intensities conform to first-order kinetics, and the process under dark conditions conform to zero-order kinetics. Thus, I. galbana can be used in the in-situ bioremediation of polluted seawater by phenol.     

Foundation and application of background trend line of China’s inbound tourism

Modern inbound tourism in China has been developing for 20 years, a trend line of inbound tourists in statistical data began to show. This paper introduces the concept of tourism background trend line, and explores its two functions as a barometer in demonstrating fluctuation in the tourism economy and as a forecaster in forecasting tourism development. The tourism background trend line is a new concept, the word “background” derived from environment science, refers to the “trend line” which reflects the dynamic curve or dynamic equation of tourism development without considering the impact of unexpected incidents. The introduction of this concept was inspired by Karl Marx’s comments on the relation between value and price. Tourism background trend line reflects the summary of multiple factors involving tourism resources, tourism demand, population growth, the scale and speed of economic development, and the spatial interaction between tourism origins and destimations. It demonstrates the natural and stable trend and the temporal law of tourism development in a country or region. The tourism statistical curve is at random, susceptible to disruptions and disturbances from serious political, economic and environmental happenings, but it always fluctuates around the background line. Tourism background line can reveal the potential of a country’s tourism development. Compared with the statistical line, it can be used as a “barometer” indicating ups and downs of tourism industry in the past. When naturally extended, the background trend line also can be used for forecasting the trend of tourism development in future. In this paper, 4 tourism background trend lines of China’s inbound tourists, i. e. foreign tourists, Hong Kong/Macao/Taiwan tourists, overseas Chinese tourists and total tourists from abroad, were established with statistical data from 1978 to 1996. And the impacts of the Political Event in 1989 (or Tiananmen Square Incident) on China’s inbound tourism were evaluated. The result shows that the impact of the Event was not limited within one year, but it stretched over 3 years. The total loss was 20 million in tourist arrivals and $1620 million in foreign currency income. The paper also studied the trend of China’s inbound tourism in the next 4 years. Foundation item: Under the auspices of National Natural Science Foundation of China. Brography: SUN Gen-nian(1961 —), male, a native of Xi’an City, Shaanxi Province, master of science, associate professor. His research interests include environment science, geography modeling and MIS for tourism.     

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Preparation and biological efficacy of haddock bone calcium tablets

To investigate the possible use of waste products obtained after processing haddock, the present study prepared haddock bone calcium powder by NaOH and ethanol soaking (alkalinealcohol method) and prepared haddock bone calcium tablets using the powder in combination with appropriate excipients. The biological efficacy of the haddock bone calcium tablets was investigated using Wistar rats as an experiment model. Results show that the optimal parameters for the alkalinealcohol method are: NaOH concentration 1 mol/L, immersion time 30 h; ethanol concentration 60%, immersion time 15 h. A mixture of 2% polyvinylpyrrolidone in ethanol was used as an excipient at a ratio of 1:2 to full-cream milk powder, without the use of a disintegrating agent. This process provided satisfactory tablets in terms of rigidity and taste. Animal studies showed that the haddock bone calcium tablets at a dose of 2 g·kg⁻¹·d⁻¹ or 5g·kg⁻¹·d⁻¹ significantly increased blood calcium and phosphorus levels and bone calcium content in rats. Therefore, these tablets could be used for calcium supplementation and prevent osteoporosis. Although the reasons of high absorption in the rats fed with haddock bone calcium tablets are unclear, it is suggested that there are some factors, such as treatment with method of alkaline-alcohol or the added milk, may play positive roles in increasing absorption ratio.     

Improvement of scattering correction for in situ coastal and inland water absorption measurement using exponential fitting approach

The absorption coefficient of water is an important bio-optical parameter for water optics and water color remote sensing. However, scattering correction is essential to obtain accurate absorption coefficient values in situ using the nine-wavelength absorption and attenuation meter AC9. Establishing the correction always fails in Case 2 water when the correction assumes zero absorption in the near-infrared(NIR) region and underestimates the absorption coefficient in the red region, which affect processes such as semi-analytical remote sensing inversion. In this study, the scattering contribution was evaluated by an exponential fitting approach using AC9 measurements at seven wavelengths(412, 440, 488, 510, 532, 555, and 715 nm) and by applying scattering correction. The correction was applied to representative in situ data of moderately turbid coastal water, highly turbid coastal water, eutrophic inland water, and turbid inland water. The results suggest that the absorption levels in the red and NIR regions are significantly higher than those obtained using standard scattering error correction procedures. Knowledge of the deviation between this method and the commonly used scattering correction methods will facilitate the evaluation of the effect on satellite remote sensing of water constituents and general optical research using different scatteringcorrection methods.