Effects of dietary carbohydrates sources on lipids compositions in abalone, Haliotis discus hannai Ino

A study was conducted to evaluate the effects of dietary carbohydrates on triglyceride, cholesterol and fatty acid concentrations in abalone, Haliotis discus hannai Ino. Six semi-purified diets with different carbohydrates (dextrin, heat-treated wheat starch, wheat starch, corn starch, tapioca starch and potato starch, respectively), all containing a carbohydrate level of 33.5%, were fed to abalone (initial shell length: 29.98 mm ± 0.09 mm; initial weight: 3.42 g ± 0.02 g) for 24 weeks in a recirculation system. The results indicate that serum triglyceride concentrations were significantly (P < 0.05) higher in the abalone fed with dextrin, heat-treated wheat starch and wheat starch than those fed with corn starch, and serum cholesterol concentrations were significantly (P < 0.05) higher in the abalone fed with dextrin, heat-treated wheat starch than those fed with corn starch. Fatty acid C20:4n-6 in the foot muscles were significantly (P < 0.05) lower in the abalone fed with dextrin than those fed with wheat starch, corn starch, tapioca starch and potato starch. Fatty acid C20:4n-6 in hepatopancreas was significantly (P < 0.05) lower in abalone fed with heat-treated wheat starch than those fed with corn starch, tapioca starch and potato starch. Fatty acid C22:6n-3 in the foot muscles were significantly (P < 0.05) lower in the abalone fed with dextrin and heat-treated wheat starch than those fed with wheat starch and potato starch.     

Vertical variations of dissolved carbohydrates in the North Yap Trench

Journal of Oceanology and Limnology - The concentrations of dissolved monosaccharides (MCHO), polysaccharides (PCHO), and total carbohydrates (TCHO) in the seawater samples collected from the north...     

Dormant stages of crustaceans as a mechanism of propagation in the extreme and unpredictable environment in the Crimean hypersaline lakes

A pool of dormant stages of planktonic organisms in saline lakes is a substantial component in the plankton communities;we need to take it into account to understand plankton dynamics.Hypersaline water bodies in Crimea,the largest peninsula in the Black Sea,constitute a very characteristic and peculiar habitat type in the region.We examined the presence of crustacean resting stages in sediments of dried up sites of the Crimean hypersaline lakes.Sediment samples were taken in 9 different lakes.Experiments performed on the hatching of these resting stages showed the presence of Moina salina(Cladocera),parthenogenetic Artemia and Artemia urmiana(Anostraca),Eucypris mareotica( inflata)(Ostracoda),and Cletocamptus retrogressus(Harpacticoida).Comparing the experimental results obtained with clean dried brine shrimp cysts and those kept in sediment samples,it was noted that clean cysts hatched much faster than those from sediments did.Some components in bottom sediments slow down and desynchronize hatching from resting eggs in different groups of crustaceans.The sediments of different lakes inhibited the nauplii output from Artemia and ostracod resting eggs to different degrees.More data are needed before we can discuss the reasons of this inhibition.The nonsynchronous output of active stages from the bottom resting ones may be an adaptation that allows crustacean species to exist in extreme and unpredictably changing environments,avoiding the risk that all may emerge at once under unsuitable conditions.     

Studies of community structure and seasonal dynamics of planktonic copepods in saline-alkaline ponds

Abstracts Species abundance and seasonal succession of copepods in saline-alkaline ponds were studied in Zhaodian Fish Farm, Gaoqing County, Shandong Province, from 5 April 1997 to 1 September 1998. The results indicated that in the conditions of salinity ranging from 1.36 to 20 g/L, total alkalinity changing from 2.4 to 7.2 mmol/L and pH 8-9, zooplankton in saline-alkaline ponds was composed of freshwater salt-tolerated species or halophile species, some of which are halobiont species and usually occurs in freshwater. In our study, copepods were predominant in many fish-culture ponds and all control ponds without fishes in spring, late autumn and early winter. Dominant species of copepods were Sinocalanus tenellus, Cyclops vicinus, Thermocyclops taihokuensis. The biomass of copepods in the control ponds without fishes was higher than that of the fish-culture ponds.     

Spatiotemporal variation of dissolved carbohydrates and amino acids in Jiaozhou Bay,China

Surface seawater samples were collected from Jiaozhou Bay,China,during six cruises(MarchMay 2010,September-November 2010) to study the distribution of dissolved organic matter including dissolved organic carbon(DOC),total dissolved carbohydrates,namely monosaccharides(MCHO) and polysaccharides(PCHO) and total hydrolysable amino acids.These included dissolved free amino acids(DFAA) and combined amino acids(DCAA).The goal was to investigate possible relationships between these dissolved organic compounds and environmental parameters.During spring,the concentrations of MCHO and PCHO were 9.6(2.8-22.6) and 11.0(2.9-42.5) μmol C/L,respectively.In autumn,MCHO and PCHO were 9.1(2.6-27.0) and 10.8(2.4-25.6) μmol C/L,respectively.The spring concentrations of DFAA and DCAA were 1.7(1.1-4.1) and 7.6(1.1-31.0) μmol C/L,respectively,while in autumn,DFAA and DCAA were 2.3(1.1-8.0) and 3.3(0.6-7.2) μmol C/L,respectively.Among these compounds,the concentrations of PCHO were the highest,accounting for nearly a quarter of the DOC,followed by MCHO,DCAA and DFAA.The concentrations of the organic compounds exhibited a decreasing trend from the coastal to the central regions of the bay.A negative correlation between concentrations of DOC and salinity in each cruise suggested that riverine inputs around the bay have an important impact on the distribution of DOC in the surface water.A significant positive correlation was found between DOC and total bacteria count in spring and autumn,suggesting bacteria play an important role in the marine carbon cycle.     

Distribution characteristics of lipids in hadal sediment in the Yap Trench

In this study, cored sediment samples collected by the Jiaolong Submersible at 6 779 m depth from the hadal zone of the Yap Trench in May 2016 were sliced in 1-cm interval from top to bottom, and lipids in each sediment layer were quantitatively and qualitatively analyzed. The vertical distribution profiles of the lipids in the sediment sample, their main existing forms, and their possible sources were investigated. The results show that the concentration of lipid in the surface sediment was the highest with the carbon number from 12 to 27, dominated by medium and short-chain lipids. The total concentration of fatty acids in surface sediment was much higher than those in the offshore and deep-sea areas, being up to 325.77 μg/g due to the funnel effect caused by the “V” terrain of the trench. Fatty acid 18.0 was the most abundant lipids in the sediment sample. Abnormal high concentrations of fatty acid 18.ω7 and alkanes indicated the existence of hydrothermal fluids in the study area. In addition, saturated fatty acids and monounsaturated fatty acids existed mainly in free form, and polyunsaturated fatty acids existed mainly in bound form. Most of the alkanes were in bound form, and their major source was autochthounous input. The carbon number of alcohols in the sediment sample ranged from 12 to 20, mainly existed in bound form. The source of fatty acids was mainly autochthonous input, and the neutral lipids had both marine and terrestrial origin. This is the first study of lipids in hadal sediment of the Yap Trench. The results will promote deeper understanding of organic carbon cycle in marine environment.     

Morphotaxonomy and seasonal distribution of planktonic and benthic Prorocentrales in Karachi waters, Pakistan Northern Arabian Sea

Morphotaxonomy and seasonal abundance of dinoflagellates of the genera Prorocentrum and Mesoporos(Prorocentrales) were studied from nutrient-rich waters,Karachi Harbor and the mouth of the Manora Channel,Pakistan during May 2002-July 2003.Using both light and scanning electron microscopy,13 species of Prorocentrales were identified according to cell shape,size,ornamentation of thecal plates,and architecture of apical platelets,apical pore area,marginal pores,and intercalary bands.P.sigmoides,P.arcuatum,P.scutellum,P.donghaiense,P.balticum,P.minimum,P.emarginatum,P.lima,P.faustiae,and Mesoporos perforatus constitute new records for sindh coast of Pakistan.The most abundant species were P.minimum/P.balticum(4.5×103 cells/L),P.micans(1.1×103 cells/L),P.gracile/P.sigmoides(2.5×102 cells/L) and P.donghaiense(6.6×103 cells/L) at temperatures of 29-31℃ and salinities of 35-40.Maximum abundance was observed in winter and lower abundance in summer.There was no significant change in the distribution of species between stations except for the benthic species which occurred close to Karachi Harbor waters.Significant positive correlations were observed between Prorocentrum spp.and temperature(R 2 =0.27) and negative correlations with salinity(R 2 =-0.32) except for P.minimum and P.emarginatum which has negative correlation with temperature(R 2 =-0.24) and positive with salinity(R 2 =0.08,0.19).The finding of potential okadaic-acid producing species of benthic Prorocentrum call for monitoring for possible human health problems in this region.     

Reproductive rate of a marine planktonic copepodLabidocera euchaet a Giesbrecht in Xiamen Harbor

Reproductive rate of a marine planktonic copepodLabidocera euchaeta Giesbrecht in Xiamen Harbor bor was studied during the period from April 1986 to August 1987. Results showed that rate of egg production (F, eggs/(♀, d)) was positively correlated with temperature (T,^oC) and body weight of spawning female (Wc, μgC) as:F=0.0637 (T−7)^0.7445 Wc. Each month a maximum rate of egg production occurred at one of the five experimental temperatures (10°, 15°, 25°, and 30°C) near the monthly mean field temperature. The available food density (P, μgC/ml) had significant effect on specific rate of egg production (Fc, %C ♀/d) as:Fc=0.1732 (P-0.0179)/P. The gross efficiency of egg production of ca 62% in carbon was independent of food density snd ingestion rate. Observations on in situ egg production and hatching rate demonstrated that this species reproduces continuously all the year round, with summer as its high reproductive season and winter a low one. Ten generations a year is also suggested based on the number of the peaks of average population egg production rate. This agreed well with the result obtained from the generation-time equations. Egg-laying pattern was also observed and discussed.     

缝洞型碳酸盐岩靶向酸压目标体分类与建模

塔河油田碳酸盐岩井周缝洞型储集体靶向酸压改造工艺针对性强,对于靶向目标精准定位与表征要求很高。围绕井周酸压增储提效,精准定量表征井筒附近储集体主应力方位、距离、规模、空间分布成为亟待解决的关键问题。从井孔与井周储层识别角度切入,采用一维资料-三维资料,多方法结合定量识别与刻画不同储层类型,在分类表征基础上,实现了模型的分类整合,构建了单元级次的多类型缝洞融合储层模型。然后,在单元级次模型基础上,创建了井周不同范围的可视化三维定量模型构建技术方法,建立了实际单井定量模型,并基于酸压靶向目标体三级八因素分类划分指标体系,确定了7种类型靶向目标体及适应的酸压技术方法。靶向酸压工艺矿场应用显示:靶向酸压井措施效果显著,同样区块范围井,比常规酸压方法平均增产倍比大,显著提高了酸压工艺的针对性和靶向性,有较好的推广应用前景。      

Seasonal variation in abundance and species composition of a planktonic diatom assemblage including viable cells on the bottom in matsushima bay,northeastern japan

Seasonal variation in abundance and species composition of a planktonic diatom assemblage distributed in the water column and also settled on the bottom was investigated for the shallow coastal water in Matsushima Bay on the Pacific coast of northeastern Japan during the period from October 1999 to September 2000. A spring bloom of diatoms began in April when nutrient concentrations started to increase, indicating the importance of nutrients. Viable cells of Skeletonema costatum and Thalassiosira spp., which were the dominant species in the water column throughout the year, were also always abundant in the bottom sediment. Both populations in the water column and on the bottom fluctuated essentially in parallel. For the planktonic diatoms in shallow coastal waters to maintain their vegetative populations in the water column, it would be advantageous for them to have a seeding population of viable cells on the bottom that are easily resuspended into the upper photic layer.     

Composition and distribution of planktonic ciliates in the southern South China Sea during late summer: Comparison between surface and 75 m deep layer

Ciliates are very important components in most marine ecosystem.They are trophic link between the microbial food web and grazing food chain.In this study,ciliates were collected from 11 sites in the southern South China Sea(SCS) during August 25 to September 28,2011.Their composition and distribution at the surface and 75 m deep depth of the ocean were studied.A total of 30 species belonging to 22 genera were identified,and 22 species of 15 genera were Tintinnids.Eutintinnus fraknoii and E.stramentus were the most common species.The other dominants were strombidiids ciliates including Strombidium conicum and S.globosaneum,which were followed by the tide form,Mesodinium pulex.Ciliates abundance ranged from 46 ind L~(-1) to 368 ind L~(-1) in the open sites,46–368 ind L~(-1) at surface and 73–198 ind L~(-1) at 75 m deep layer.In the Yongshu reef,ciliates abundance ranged from 167 ind L~(-1) to 365 ind L~(-1) in the water column,similar to that in Sanya coral reef waters.Ciliates composition showed obvious difference between surface and 75 m deep layer at station S2(P 0.05),while no similar result was observed at other sites.At 75 m deep layer,salinity was negatively related to mixed layer depth(P 0.05),but positively to chlorophyll a concentration(P 0.05),indicating that the change of vertical mixing in water column influenced vertical distribution of ciliates in the southern SCS.     

隧道工程地质评价的内容和方法

结合工程隧道实际,首先阐述了隧道工程的基本地质环境和工程地质条件,然后针对隧道工程可能出现的不良地质现象和可采取的工程措施,从大气降水、围岩稳定、围岩压力、洞口稳定、隧道比选等角度探讨隧道工程地质评价的主要方法和一般内容,进行隧道工程地质评价,为隧道施工、支护提供了依据.     

Morphological Studies on the Infraciliature of a Planktonic Ciliate, Tintinnopsis brasiliensis （ Ciliophora ： Tintinina）

A poorly-described marine planktonic ciliate, Tintinnopsis brasiliensis Kofoid ＆ Campbell, 1929, collected from the Taiping Cape of Qingdao, China, was morphologically investigated based on permanent preparation after protargol impregnation and was compared with other related congeners. According to the infraciliature, three ciliary groups can be recognized, which reveals a very stable structure among specimens and denotes that the pattern of infraciliature is, apart from the features of the lorica, a highly reliable criterion for species identification.     

Comparison of lipids in organs of the starfish Asterias amurensis associated with different treatments

Lipids were extracted from organs of the starfish Asterias amurensis associated with different treatments(raw-control,boiling and heating),and then analyzed for lipid content,lipid oxidation index,lipid classes and fatty acid composition.Results showed that boiling softened the hard starfish shells,thus facilitating the collection of starfish organs.As compared with raw organs,the boiled organs had lower water content and higher lipid content,possibly due to the loss of water-holding capacity caused by protein denaturation.Both boiling and heating increased the peroxide value(PV),thiobarbituric acid(TBA) value and carbon value(CV) of lipids.Despite slight increases in the content of complex lipids,associated lipid composition had no substantial variations upon boiling and heating.For simple lipids,the content of 1,2-diglyceride decreased in boiled and heated organs,with free fatty acids observed on thin layer chromatography(TLC).However,neither boiling nor heating significantly changed the fatty acid compositions of simple or complex lipids in starfish organs,suggesting that these two treatments had no significant effects on complex lipids in starfish organs.Together,our results indicated that boiling of starfish soon after capture facilitated the handling and extraction of useful complex lipids consisting of abundant glucosylceramide and eicosapentaenoic acid(EPA)-bounded phospholipids.     

Decomposition of algal lipids in clay-enriched marine sediment under oxic and anoxic conditions

A series of laboratory incubation experiments were conducted to examine the decomposition of algal organic matter in clay-enriched marine sediment under oxic and anoxic conditions.During the 245-day incubation period,changes in the concentrations of TOC,major algal fatty acid components (14:0,16:0,16:1,18:1 and 20:5),and n-alkanes (C16-C23) were quantified in the samples.Our results indicate that the organic matters were degraded more rapidly in oxic than anoxic conditions.Adsorption of fatty acids onto cla...     

Decomposition of algal lipids in clay-enriched marine sediment under oxic and anoxic conditions

A series of laboratory incubation experiments were conducted to examine the decomposition of algal organic matter in clay-enriched marine sediment under oxic and anoxic conditions. During the 245-day incubation period, changes in the concentrations of TOC, major algal fatty acid components (14:0, 16:0, 16:1, 18:1 and 20:5), and n-alkanes (C₁₆–C₂₃) were quantified in the samples. Our results indicate that the organic matters were degraded more rapidly in oxic than anoxic conditions. Adsorption of fatty acids onto clay minerals was a rapid and reversible process. Using a simple G model, we calculated the decomposition rate constants for TOC, n-alkanes and fatty acids which ranged from 0.017–0.024 d⁻¹, 0.049–0.103 d⁻¹ and 0.011 to 0.069 d⁻¹, respectively. Algal organic matter degraded in two stages characterized by a fast and a slow degradation processes. The addition of clay minerals montmorillonite and kaolinite to the sediments showed significant influence affecting the decomposition processes of algal TOC and fatty acids by adsorption and incorporation of the compounds with clay particles. Adsorption/association of fatty acids by clay minerals was rapid but appeared to be a slow reversible process. In addition to the sediment redox and clay influence, the structure of the compounds also played important roles in affecting their degradation dynamic in sediments.     

Sedimentary ladderane core lipids as potential indicators of hypoxia in the East China Sea

Anammox (anaerobic ammonium oxidation) is an important process in many suboxic to anoxic marine environments for converting fixed nitrogen to N₂, and has a major impact on the marine nitrogen cycle. Ladderane core lipids have been utilized as an indicator of the contribution of anammox to the marine nitrogen cycles. However, such studies have not been reported for the China seas and little is known about the importance of anammox within the nitrogen cycle of these marginal seas. In the research reported here, the ladderane core lipid contents of 17 surface sediment samples from the East China Sea are reported, and their spatial distribution is investigated. C₁₈ -[5]-ladderane FAME, C₂₀-[5]-ladderane FAME and C₂₀-[3]-ladderane FAME have all been detected, suggesting that the anammox bacteria are widely present within the study area. The total contents of the three ladderane lipids (ΣFAMEs) range from 24–355 ng/g (weight of dry sediments), with higher contents occurring in the Minzhe Mud Zone and broadly coincident with the spatial distribution of hypoxia. It is suggested that the sedimentary ladderane core lipids are mainly produced in the water column and their sedimentary contents can be used as indicators of water column hypoxia.     

Sea surface temperature and salinity reconstruction based on stable isotopes and Mg/Ca of planktonic foraminifera in the western Pacific Warm Pool during the last 155 ka

Changes in sea surface temperature(SST), seawater oxygen isotope(δ 18 O sw), and local salinity proxy(δ 18 O sw-ss) in the past 155 ka were studied using a sediment core(MD06-3052) from the northern edge of the western Pacifi c Warm Pool(WPWP), within the fl ow path of the bifurcation of the North Equatorial Current. Our records reveal a lead-lag relationship between paired Mg/Ca-SST and δ 18 O during Termination II and the last interglacial period. Similarity in SST between our site and the Antarctic temperature proxy and in CO 2 profi le showed a close connection between the WPWP and the Antarctic. Values of δ 18 O sw exhibited very similar variations to those of mean ocean δ 18 O sw, owing to the past sea-level changes on glacial-interglacial timescale. Calculated values of δ 18 O sw-ss refl ect a more saline condition during high local summer insolation(SI) periods. Such correspondence between δ 18 O sw-ss and local SI in the WPWP may refl ect complex interaction between ENSO and monsoon, which was stimulated by changes in solar irradiance and their infl uence on the local hydrologic cycle. This then caused a striking reorganization of atmospheric circulation over the WPWP.     

Contents and benthic fluxes of nutrients in sediment pore water of the Southern Ocean

The concentration and profile characters of nutrients in sediment pore water of the South Ocean, sampled during the cruise of CHINARE-18 of the austral summer 2001/02 were determined. The results show that the content of SiO3-Si and NH4-N were much higher than other nutrients. The profile of SiO3-Si was characterized with sharp gradients near the sediment-water interface, profile distribution of silicate show that during the diagenetic reaction of silicate, dissolution was the main part. The organic matter decomposition was occurred under the anaerobic condition. Benthic fluxes of SiO3-Si, NH4-N were from pore water to overlying water, and regeneration of silicate and ammonium were the main part of the nutrient regeneration from the pore water of the study area.     

Degradation of lipids in seasonal hypoxic seawater under different oxygen saturation

Journal of Oceanology and Limnology - The hypoxic phenomena of seawater have been found in the Changjiang esturay and its adjacent area for several decades. To study organic matter degradation in...