Persistent organic pollutants and stable isotopes in biopsy samples (2004/2006) from Southern Resident killer whales

“Southern Resident” killer whales include three “pods” (J, K and L) that reside primarily in Puget Sound/Georgia Basin during the spring, summer and fall. This population was listed as “endangered” in the US and Canada following a 20% decline between 1996 and 2001. The current study, using blubber/epidermis biopsy samples, contributes contemporary information about potential factors (i.e., levels of pollutants or changes in diet) that could adversely affect Southern Residents. Carbon and nitrogen stable isotopes indicated J- and L-pod consumed prey from similar trophic levels in 2004/2006 and also showed no evidence for a large shift in the trophic level of prey consumed by L-pod between 1996 and 2004/2006. ∑PCBs decreased for Southern Residents biopsied in 2004/2006 compared to 1993–1995. Surprisingly, however, a three-year-old male whale (J39) had the highest concentrations of ∑PBDEs, ∑HCHs and HCB. POP ratio differences between J- and L-pod suggested that they occupy different ranges in winter.     

Estimation of southern resident killer whale exposure to exhaust emissions from whale-watching vessels and potential adverse health effects and toxicity thresholds

Southern resident killer whales in British Columbia and Washington are exposed to heavy vessel traffic. This study investigates their exposure to exhaust gases from whale-watching vessels by using a simple dispersion model incorporating data on whale and vessel behavior, atmospheric conditions, and output of airborne pollutants from the whale-watching fleet based on emissions data from regulatory agencies.Our findings suggest that current whale-watching guidelines are usually effective in limiting pollutant exposure to levels at or just below those at which measurable adverse health effects would be expected in killer whales. However, safe pollutant levels are exceeded under worst-case conditions and certain average-case conditions. To reduce killer whale exposure to exhaust we recommend: vessels position on the downwind side of whales, a maximum of 20 whale-watching vessels should be within 800 m at any given time, viewing periods should be limited, and current whale-watch guidelines and laws should be enforced.     

High thresholds for avoidance of sonar by free-ranging long-finned pilot whales (Globicephala melas)

The potential effects of exposing marine mammals to military sonar is a current concern. Dose–response relationships are useful for predicting potential environmental impacts of specific operations. To reveal behavioral response thresholds of exposure to sonar, we conducted 18 exposure/control approaches to 6 long-finned pilot whales. Source level and proximity of sonar transmitting one of two frequency bands (1–2 kHz and 6–7 kHz) were increased during exposure sessions. The 2-dimensional movement tracks were analyzed using a changepoint method to identify the avoidance response thresholds which were used to estimate dose–response relationships. No support for an effect of sonar frequency or previous exposures on the probability of response was found. Estimated response thresholds at which 50% of population show avoidance (SPL_max = 170 dB re 1 μPa, SEL_cum = 173 dB re 1 μPa² s) were higher than previously found for other cetaceans. The US Navy currently uses a generic dose–response relationship to predict the responses of cetaceans to naval active sonar, which has been found to underestimate behavioural impacts on killer whales and beaked whales. The navy curve appears to match more closely our results with long-finned pilot whales, though it might underestimate the probability of avoidance for pilot-whales at long distances from sonar sources.     

Ballast water as a vector of coral pathogens in the Gulf of Mexico: the case of the Cayo Arcas coral reef

The discharge of nutrients, phytoplankton and pathogenic bacteria through ballast water may threaten the Cayo Arcas reef system. To assess this threat, the quality of ballast water and presence of coral reef pathogenic bacteria in 30 oil tankers loaded at the PEMEX Cayo Arcas crude oil terminal were determined. The water transported in the ships originated from coastal, oceanic or riverine regions. Statistical associations among quality parameters and bacteria were tested using redundancy analysis (RDA). In contrast with coastal or oceanic water, the riverine water had high concentrations of coliforms, including Vibrio cholerae 01 and, Serratia marcescens and Sphingomona spp., which are frequently associated with “white pox” and “white plague type II” coral diseases. There were also high nutrient concentrations and low water quality index values (WQI and TRIX). The presence of V. cholerae 01 highlights the need for testing ballast water coming from endemic regions into Mexican ports.     

Dibenzofurans a greater global pollutant than dioxins ? : Evidence from analyses of open ocean killer whale

Three specimens of killer whales (Orcinus orca), an open ocean carnivore, were analysed for extremely toxic polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) to understand their long-range distribution patterns. Several PCDF congeners, including the highly toxic 2,3,7,8-tetra- and 2,3,4,7,8-penta-CDFs were identified in the blubber of those specimens. The PCDF isomeric pattern in killer whale is more complex than the reported patterns in humans and birds, indicating the weaker metabolic potency of killer whales for these toxic compounds. High levels of PCBs (about 400 mg kg⁻¹) have also been detected in those specimens. The 2,3,7,8-substituted PCDF congeners identified in commercial PCBs were also found in killer whale, indicating PCBs as the possible source. Isomer-specific and trace level determinations of PCDD in killer whale, revealed no detectable quantities. The detection of comparatively high levels ( > 300 ng kg⁻¹) of PCDFs and undetection of PCDDs in open ocean killer whales suggest that PCDFs are more ubiquitous than PCDDs.     

Disturbance of recruitment success of mantis shrimp in Tokyo Bay associated with effects of hypoxia on the early life history

We investigated effects of severe hypoxia (dissolved oxygen <1 ml l⁻¹) on recruitment of mantis shrimp Oratosquilla oratoria in Tokyo Bay. Ten-year field surveys were conducted to examine quantitative relationships in annual mean densities of larvae and juveniles, and spatial distribution of juveniles and severe hypoxia. There was no significant correlation between annual mean densities of larvae and juveniles, suggesting that mortality during larval or juvenile stages varies among years, which might have regulated abundance of young-of-the-year juveniles. Juvenile density was low in the severely hypoxic area, implying that hypoxia could affect survivals and spatial distribution of juveniles. Meanwhile, there are yearly fluctuations in juvenile density in normoxic areas of both northern and southern part of the bay. This evidence suggests that abundance of post-settled juveniles might have been determined by not only effects of hypoxia, but also other factors influencing mortality during the early life stages.     

Salmonella: The forgotten pathogen: Health hazards of compliance with European Bathing Water Legislation

The increasing demands on recreational waters have made microbial contamination a matter of public and scientific concern. This study aimed to search for Salmonella spp. in waters classified according EU Directive 2006/7/EC, in order to assess associations between its prevalence and the concentration of the non-pathogenic new faecal indicators: Escherichia coli and intestinal enterococci. Although a statistically significant association was observed Salmonella was detected on beaches classified as “Good” (9.3%) and “Excellent” (14.4%) which compromises the idea that faecal indicators can be predictors of pathogens.Attending the high prevalence of Salmonella found (23.1%) it seemed important to improve the efficiency of the conventional analytical method (ISO 6340:1995), comparing its draft with SML-VIDAS Salmonella and two new chromogenic media: AES Salmonella Agar Plate (ASAP) and Simple Method Salmonella (SMS). ASAP showed the higher efficiency and can be recommended for a faster detection and presumptive identification of salmonellae in bathing waters.     

Comparing plastic ingestion in juvenile and adult stranded short-tailed shearwaters (Puffinus tenuirostris) in eastern Australia

Numerous species of seabirds have been shown to ingest anthropogenic debris, but few studies have compared ingestion rates between adults and juveniles of the same species. We investigated marine debris ingestion by short-tailed shearwaters (Puffinus tenuirostris) obtained through two stranding events on North Stradbroke Island, Australia in 2010 (n = 102; adult) and 2012 (n = 27; juveniles). Necropsies were conducted and solid contents found in guts were categorized into type and color. Over 67% of birds ingested anthropogenic debris: 399 pieces of debris were identified. We found no significant relationship between body condition of birds which had ingested anthropogenic debris and those that had not. Juvenile birds were more likely to ingest debris than were adult birds and juveniles ingested significantly more pieces of debris than did adults. Male and female birds ingested similar amounts and weights of debris. To determine if P. tenuirostris actively selects for certain types of debris, we compared ingested debris to samples obtained from boat-based tows. Significant differences were found, suggesting that the birds select for hard plastic, rubber and balloons.     

An example of application: The ecological “natural flow regime” paradigm in hydroclimatology

According to the concept of “natural flow regime,” introduced and developed in the 1990's in aquatic ecology, streamflow can be described using five basic characteristics: magnitude, frequency, duration, period of occurrence and variability. A sixth could be added to these, namely, distribution curve. Our study, which focused exclusively on the temporal variability of these six characteristics, had these objectives: (1) to compare their stationarity, (2) to determine the links among these characteristics and (3) to analyze, for the first time, their relationship to six climatic indices (Atlantic Multidecadal Oscillation, Artic Oscillation, North Atlantic Oscillation, Niño3.4, Pacific decadal Oscillation and Southern Oscillation Index). To do this, we used the Vermillon River (2670 km²) as an example, analyzing the streamflow of heavy spring floods (equal to or greater than the annual flood streamflow), as measured between 1934 and 2000.     

On a fractional derivative form of the Green-Ampt infiltration model

The Green-Ampt model for infiltration into homogeneous soils predicts a monotonically decreasing infiltration rate and a wetting front that initially advances as the square root of time. Infiltration in heterogeneous soils, however, can exhibit non-monotonic infiltration rates and wetting front advances that differ from the square root of time (“anomalous diffusion”).Here it is postulated, that if the length scales of the heterogeneities can be assumed to be power law distributed, it may be appropriate to model infiltration in heterogeneous soils in terms of fractional derivatives. Then, by expressing the hydraulic flux as a Caputo fractional derivative (order 0 < α ? 1) of the head, a fractional Green-Ampt infiltration model is obtained. It is shown that solutions of this model predict non-monotonic and anomalous diffusion behaviors consistent with observations in field infiltration trials; a finding that indicates that a non-local moisture flux model, based on fractional derivatives, is a plausible model for describing infiltration into heterogeneous soils.     

On core surface flows inferred from satellite magnetic data

Satellite-data allows the magnetic field produced by the dynamo within the Earth’s core to be imaged with much more accuracy than previously possible with only ground-based data. Changes in this magnetic field can in turn be used to make some inferences about the core surface flow responsible for them. In this paper, we investigate the improvement brought to core flow computation by new satellite-data based core magnetic field models. It is shown that the main limitation now encountered is no longer the (now high) accuracy of those models, but the “non-modelled secular variation” produced by interaction of the non-resolvable small scales of the core flow with the core field, and by interaction of the (partly) resolvable large scales of the core flow with the small scales of the core field unfortunately masked by the crustal field. We show how this non-modelled secular variation can be taken into account to recover the largest scales of the core flow in a consistent way. We also investigate the uncertainties this introduces in core flows computed with the help of the frozen-flux and tangentially geostrophic assumptions. It turns out that flows with much more medium and small scales than previously thought are needed to explain the satellite-data-based core magnetic field models. It also turns out that a significant fraction of this flow unfortunately happens to be non-recoverable (being either “non-resolvable” because too small-scale, or “invisible”, because in the kernel of the inverse method) even though it produces the detectable “non-modelled secular variation”. Applying this to the Magsat (1980) to Ørsted (2000) field changes leads us to conclude that a flow involving at least strong retrograde vortices below the Atlantic Hemisphere, some less-resolved prograde vortices below the Pacific Hemisphere, and some poorly resolved (and partly non-resolvable) polar vortices, is needed to explain the 1980-2000 satellite-era average secular variation. The characteristics of the fraction of the secular variation left unexplained by this flow are also discussed.     

Using beamforming for the global network location

The aim of this study is the design and trial of a novel sparse network beamforming (NB) technique to improve earthquake location. For this purpose bulletin phase arrival time data were processed via the use of complex exponents form and then used in an optimization via grid-search to search for the maximum semblance in hypocenter space. The use of the robust semblance statistic, provides reliable location results. The NB location results for a set of test events were compared to standard iterative ISC location procedure “iscloc” and its prototype the Jeffreys maximum likelihood estimator. For this purpose a data-base of 139 reference ground-truth events was extracted from the catalog of the International Seismological Centre (ISC) (97 GT5 events, assumed earthquakes, and 42 GT0 nuclear tests). The tuned NB procedure has shown excellent location results for events with a “good” ISC location and demonstrated the large epicenter deviations have been decreased in “bad” cases. Further developments of the algorithm would include allowances for 3D earth structure and a priori site-specific information.     

Stable isotope ratios of carbon,nitrogen and oxygen in killer whales (Orcinus orca) stranded on the coast of Hokkaido,Japan

We analyzed δ¹³C, δ¹⁵N and δ¹⁸O in the muscle and liver from killer whales stranded on the coast of Japan. The δ¹⁵N values in the muscle samples from calves were apparently higher than those in their lactating mothers, suggesting that nursing may result in the higher δ¹⁵N values in the muscle samples of calves. The δ¹⁵N value in the muscle samples of male and female whales, except for the calves, were positively correlated with the δ¹³C values and body length, suggesting that the increases in δ¹⁵N were due to the growth of the whales and increase in their trophic level. In contrast, the δ¹⁸O values in the muscle samples of female whales except for the calves were negatively correlated with the δ¹³C and δ¹⁵N values. The δ¹⁸O may be lower in whales occupying higher trophic positions (δ¹⁵N), although it might also be affected by geographic and climatic conditions.     

Cadmium distribution in coastal sediments and mollusks of the US

Cadmium (Cd) concentrations in the coastal United States were assessed using the National Status and Trends (NS&T) Mussel Watch dataset, which is based on the analysis of sediments and bivalves collected from 280 sites since 1986. Using the 1997 sediment data, Pearson correlation (r = 0.44, p < 0.0001) suggested that Cd distributions in sediment can, be to some extent, explained by the proximity of sites to population centers. The 2003 tissue data indicated that “high” Cd concentrations (greater than 5.6 μg/g dry weights [dw] for mussel and 5.4 μg/g dw for oysters) were related to salinity along the East and Gulf coasts. Along the West coast, however, these “high” sites appeared to be related to upwelling phenomenon. Additionally, sedimentary diagenesis was found to be the most likely explanation of why sediment and mollusk Cd content were not well correlated.     

Decade-long monitoring reveals a transient distortion of baseline butyltin bioaccumulation pattern in gastropods

Worldwide measures to restrict tributyltin (TBT) in antifouling paints have been legislated for decades, and were upgraded to a total ban on September 2008. With a view to test the response of coastal biota to changing pollution, since 1996 we have determined the concentration of TBT and derivatives di- and mono-butyltin (DBT and MBT) in NW Spain populations of two gastropods of contrasting biology, the rock-snail Nucella lapillus (n = 18) and the mud-snail Nassarius reticulatus (n = 24). TBT pollution in the study area has decreased consistently and considerably over time. In addition, the baseline butyltin (BT) bioaccumulation patterns showed a marked but transient distortion. These field observations are consistent with BT desorption from sediments, a natural phenomenon that is now to be expected in developing countries recently subject to the global TBT ban.     

Parametric analysis of single pile response in laterally spreading ground

Extensive damage to pile-supported structures has been witnessed in several recent earthquakes (Chi-Chi, 1999; Kobe, 1995, etc.), as a result of liquefaction-induced lateral spreading of slightly sloping ground or free-face topographic irregularities. This paper presents a parametric analysis of the basic pile and soil parameters, as well as the pile-soil interaction mechanisms affecting the response of single piles subjected to such lateral spreading, based on numerical simulation with the nonlinear P-y method. In parallel, a set of design charts and analytical relations is established, for approximate computation of maximum pile deflections and bending moments, using a “theory guided” multi-variable statistical analysis of the numerical predictions. Three different combinations (design cases) of pile head constraints and soil conditions were considered, which are commonly encountered in practice. The overall accuracy of the proposed analytical relations is evaluated against experimental results from seven centrifuge and five large shaking table experiments.     

Entanglement of Steller sea lions (Eumetopias jubatus) in marine debris: Identifying causes and finding solutions

Entanglement in marine debris is a contributing factor in Steller sea lion (SSL; Eumetopias jubatus) injury and mortality. We quantified SSL entanglement by debris type, sex and age class, entanglement incidence, and estimated population level effects. Surveys of SSL haul-outs were conducted from 2000-2007 in Southeast Alaska and northern British Columbia. We recorded 386 individuals of all age classes as being either entangled in marine debris or having ingested fishing gear. Packing bands were the most common neck entangling material (54%), followed by rubber bands (30%), net (7%), rope (7%), and monofilament line (2%). Ingested fishing gear included salmon fishery flashers (lures: 80%), longline gear (12%), hook and line (4%), spinners/spoons (2%), and bait hooks (2%). Entanglement incidence was 0.26% (SD = 0.0064, n = 69 sites). “Lose the Loop!” Simple procedures such as cutting entangling loops of synthetic material and eliminating the use of packing bands can prevent entanglements.     

Side-scan sonar estimates of the utilization of gray whale feeding grounds along Vancouver Island,Canada

Infaunal prey communities and gray whale feeding excavations were found at three sites along the west coast of Vancouver Island: Ahous Bay, Pachena Bay and Port San Juan. Side-scan sonar records indicated that whales disturbed up to 36% of the sea floor. The mean percentage of the bottom covered with feeding excavations was > 17% in each of the three feeding grounds. There was a positive relationship between the biomass of ampeliscid amphipod prey and the total quantity of prey consumed by gray whales from the three feeding grounds. There also was considerable annual variation in the size of feeding excavations, the total area covered by excavations, and the dispersion of excavations over the entire prey community during 3 years of observations at Pachena Bay. In general, two types of gray whale feeding records occurred in the Ampelisca tube mats. One record was composed of large complex excavations (mean > 20.5 m²): the other contained only small features(mean < 4 m²). Gray whales expanded the small feeding excavations into large, complex features by feeding along the edges of existing depressions. Edge feeding may increase the energy yield per dive as whales avoid low biomass areas inside existing excavations. It may also require less energy to lift the bottom by suction along the edge of an exposed tube mat. Gray whale feeding suspended over 1000 m³ of sediment per day. Since sediment grain size was coarser inside feeding excavations (mean = 0.160 mm) compared to outside (mean = 0.124 mm), gray whales may help to maintain the mud poor fine sand habitat of its primary benthic prey, Ampelisca, by winnowing away finer sediments. Certain infaunal prey types (e.g. onuphid polychaetes) are less resilient to gray whale predation than others (e.g. Ampelisca) as a result of their local distribution patterns and life history.     

Comparative study of 17 β-estradiol on endocrine disruption and biotransformation in fingerlings and juveniles of Japanese sea bass Lateolabrax japonicus

Estrogenic contaminants in the aquatic environment are associated with endocrine disruption and feminization in fish. The effects of endocrine disrupting chemicals (EDCs) on fish have been well documented. However, very few studies have focused on 17 β-estradiol (E2) and its effects on endocrine system and biotransformation in a single prolonged exposure. This study investigated changes in the levels of serotonin (5-hydroxytryptamine) and acetyl choline esterase (AchE) in brain, cortisol in plasma and Ethoxyresorufin-O-deethylase (EROD) activity in gill of two different size groups (fingerlings and juveniles) of Japanese sea bass (Lateolabrax japonicus) upon exposure to two sub-lethal concentrations (200 and 2000 ng L⁻¹) of E2 for 30 d. The results indicate that cortisol level and EROD activity significantly increased in both groups, whereas serotonin level increased in juveniles and decreased in fingerlings due to E2 exposure. The correlation analysis revealed that E2 significantly affected the endocrine and biotransformation systems in both age groups.     

Bank filtration in the sandy littoral zone of Lake Tegel (Berlin): Structure and dynamics of the biological active filter zone and clogging processes

This study focused on clogging processes and on the benthic microalgal and meiofaunal assemblage in the sandy littoral zone of Lake Tegel, which are significantly involved in bank filtration, in a long-term. Our approach combined field studies and “in situ” experiments to highlight the structure of the biological active filter zone as well as the mechanisms and effects of clogging in the interstices that influence the infiltration process.Campaigns to measure “in situ” infiltration rates and hydraulic potential were conducted monthly from March 2004 to April 2005. Meiofaunal abundances and fine particulate organic matter (FPOM) were determined every 6 weeks in freeze cores down to depths of 50 cm. In parallel, concentrations of carbon, nitrogen and chlorophyll a were measured in samples of unfrozen sediment cores, that were divided in 1-cm steps down to depths of ≥10 cm. Similar sediment profiles were generated for analysis of colloidal carbohydrates, extracellular polymeric substances (EPS) and proteins between December 2005 and June 2006. Electron microscopy was used to visualize biofilm structure. Long-term experiments with natural FPOM and melamine resin particles as fluorescent tracers were performed to study “in situ” particle retention and transport, respectively. Additionally seston input was quantified during a 1-week period in April 2005.Infiltration rates showed a high temporal and spatial variability, but were not correlated with hydraulic conductivities as hydraulic gradients changed a lot. Likewise a correlation between infiltration rates and hydraulic potentials was not observed, indicating clogging processes. These are triggered to a high extend by biological compounds. In addition, seston input and intermittent gas intrusion are considered to reduce the hydraulic conductivity considerably. No significant “in situ” transport of inert natural fluorescent tracers was observed. However, a complete and permanent clogging of the sandy sediment does not occur, and daily infiltration rates of 0.7-27 L m⁻² h⁻¹ (mean 9 L m⁻² h⁻¹) guarantee a sufficient water supply by bank filtration for decades.