Microbial degradation rates of small peptides and amino acids in the oxygen minimum zone of Chilean coastal waters

We found similar microbial degradation rates of labile dissolved organic matter in oxic and suboxic waters off northern Chile. Rates of peptide hydrolysis and amino acid uptake in unconcentrated water samples were not low in the water column where oxygen concentration was depleted. Hydrolysis rates ranged from 65 to 160 nmol peptide L⁻¹ h⁻¹ in the top 20 m, 8–28 nmol peptide L⁻¹ h⁻¹ between 100 and 300 m (O₂-depleted zone), and 14–19 nmol peptide L⁻¹ h⁻¹ between 600 and 800 m. Dissolved free amino acid uptake rates were 9–26, 3–17, and 6 nmol L⁻¹ h⁻¹ at similar depth intervals. Since these findings are consistent with a model of comparable potential activity of microbes in degrading labile substrates of planktonic origin, we suggest, as do other authors, that differences in decomposition rates with high and low oxygen concentrations may be a matter of substrate lability. The comparison between hydrolysis and uptake rates indicates that microbial peptide hydrolysis occurs at similar or faster rates than amino acid uptake in the water column, and that the hydrolysis of peptides is not a rate-limiting step for the complete remineralization of labile macromolecules. Low O₂ waters process about 10 tons of peptide carbon per h, double the amount processed in surface-oxygenated water. In the oxygen minimum zone, we suggest that the C balance may be affected by the low lability of the dissolved organic matter when this is upwelled to the surface. An important fraction of dissolved organic matter is processed in the oxygen minimum layer, a prominent feature of the coastal ocean in the highly productive Humboldt Current System.     

Biomass size-spectra of macrobenthic communities in the oxygen minimum zone off Chile

Estimates of macrofaunal secondary production and normalized biomass size-spectra (NBSS) were constructed for macrobenthic communities associated with the oxygen minimum zone (OMZ) in four areas of the continental margin off Chile. The presence of low oxygen conditions in the Humboldt Current System (HCS) off Chile was shown to have important effects on the size structure and secondary production of the benthic communities living in this ecosystem. The distribution of normalized biomass by size was linear (log₂–log₂ scale) at all stations. The slope of the NBSS ranged from −0.481 to −0.908. There were significant differences between the slopes of the NBS-spectra from the stations located in the OMZ (slope = −0.837) and those located outside the OMZ (slope = −0.463) (p < 0.05). The results of this study suggest that low oxygen conditions (<0.5 ml L⁻¹) appear to influence biomass size-spectra, because small organisms are better able to satisfy their metabolic demands. The annual secondary production was higher off central Chile (6.8 g C m⁻² y⁻¹) than off northern Chile (2.02 g C m⁻² y⁻¹) and off southern Chile (0.83 g C m⁻² y⁻¹). A comparison with other studies suggests that secondary production in terms of carbon equivalents was higher than in other upwelling regions.     

The habitat of mesopelagic scyphomedusae in Monterey Bay,California

In the mesopelagic zone, at depths of 200–1000 m in the Monterey Submarine Canyon, CA, medusae in three genera of scyphozoa, Atolla, Periphylla and Poralia, were observed, videotaped and collected over a 9-year period (1990–1998). Environmental data were obtained simultaneously using a remotely operated vehicle (ROV) with sensors for depth, temperature, salinity and dissolved oxygen. Shipboard measurements of these same properties at two reference stations in the region defined the local water masses and helped identify species niches using the metric of spiciness and oxygen levels of the waters in which medusae were visually “captured”. The most abundant genus of mesopelagic scyphomedusae was Atolla, found associated most strongly with the spicy (warm, salty) waters of the California Undercurrent, usually above the core of the oxygen minimum zone (OMZ; O₂>0.5 ml/l). The least abundant mesopelagic scyphomedusa was Periphylla, which occurred in more variable waters, including those with a greater contribution of fresher, colder (less spicy), subarctic water and, hence, most like those at the offshore California Current station in the most depleted oxygen zone (averaging O₂ <0.3 ml/l). Poralia was mostly confined to the densest, coldest water, with peak abundance at the lower boundary of the OMZ (i.e., 0.3< O₂<0.5 ml/l). These spiciness measures on local isopycnal surfaces within the mesopelagic zone, supported by data on dissolved oxygen concentrations, indicate highly significant but fine-scale habitat differences in species habitats in Central California waters. This in situ investigation appears to be one of only a few studies to document fine-scale, water mass affinities of mesopelagic zooplankton.     

Phytoplankton community structure in the Arabian Sea during and after the SW monsoon, 1994

Diatoms, dinoflagellates, coccolithophores, nanoflagellates, picophytoplankton and procaryote algae (Synechococcus spp. and prochlorophytes) were quantified by microscopy and flow cytometry, and their biomass determined, at 12 stations along a 1600 km transect across the Arabian Sea at the end of the SW monsoon in September, and during the inter-monsoon period of November/December 1994. The transect spanned contrasting oceanic conditions that varied from seasonally eutrophic, upwelling waters through mesotrophic, downwelling waters to permanently oligotrophic, stratified waters. The overall diversity of diatoms, dinoflagellates and coccolithophores along the transect was not significantly different between the SW monsoon and inter-monsoon. However, diatoms showed greatest diversity during the SW monsoon and coccolithophores were most diverse during the inter-monsoon. Integrated phytoplankton standing stocks during the SW monsoon ranged from 3 to 9 g C m^-2 in the upwelling eutrophic waters, from 3 to 5 g C m^-2 in downwelling waters, and from 1 to 2 g C m^-2 in oligotrophic waters. Similar phytoplankton standing stocks were found in oligotrophic waters during the inter-monsoon, but were ca. 40% lower compared to the SW monsoon in the more physically dynamic waters. Phytoplankton abundance and biomass was dominated by procaryote taxa. Synechococcus spp. were abundant (often >10⁸ cells l^-1) during both the SW monsoon and inter-monsoon, where the nitrate concentration was ⩾0.1 μ mol l^-1, and often dominated the phytoplankton standing stocks. Prochlorophytes were restricted to oligotrophic stratified waters during the SW monsoon period but were found at all stations along the transect during the inter-monsoon, dominating the phytoplankton standing stocks (>40%) in the oligotrophic region during this period. Of the nano- and micro-phytoplankton, only diatoms contributed significantly to phytoplankton standing stocks, and then only in near-shore upwelling waters during the SW monsoon. There were significant changes in the temporal composition of the phytoplankton community. In nearshore waters a mixed community of diatoms and Synechococcus spp. dominated during the SW monsoon. This gave way to a community dominated by Synechococcus spp. in the intermonsoon. In the downwelling zone, a Synechococcus spp. dominated community was replaced by a mixed procaryote community of Synechococcus spp. and prochlorophytes. In the oligotrophic stratified waters, the mix of procaryote algae was replaced by one dominated by prochlorophytes alone.     

The seasonal succession of zooplankton in the Southern Ocean south of Australia,part I: The seasonal ice zone

Between November 2001 and March 2002 an Australian/Japanese collaborative study completed six passes of a transect line in the Seasonal-Ice Zone (south of 62°S) along 140°E. Zooplankton samples were collected with a NORPAC net on 22–28 November, and a Continuous Plankton Recorder on 10–15 January, 11–12 February, 19–22 February, 25–26 February, and 10–11 March. Zooplankton densities were lowest on 22–28 November (ave=61 individuals (ind) m⁻³), when almost the entire transect was covered by sea ice. By 10–15 January sea surface temperature had increased by ∼2 °C across the transect line, and the study area was ice-free. Total zooplankton abundance had increased to maximum levels for the season (ave=1301 ind m⁻³; max=1979 ind m⁻³), dominated by a “Peak Community” comprising Oithona similis, Ctenocalanus citer, Clausocalanus laticeps, foraminiferans, Limacina spp., appendicularians, Rhincalanus gigas and large calanoid copepodites (C1–3). Total densities declined on each subsequent transect, returning to an average of 169 ind m⁻³ on 10–11 March. The seasonal density decline was due to the decline in densities of “Peak Community” taxa, but coincided with the rise of Euphausia superba larvae into the surface waters, increased densities of Salpa thompsoni, and an increased contribution of C4 to adult stages to the populations of Calanoides acutus, Calanus propinquus and Calanus simillimus. The seasonal community succession appeared to be influenced by the low sea ice extent and southward projection of the ACC in this region. The relatively warm ACC waters, together with low krill biomass, favoured high densities of small grazers during the January/February bloom conditions. The persistence of relatively warm surface waters in March and the seasonal decrease in chlorophyll a biomass provided favorable conditions for salps, which were able to penetrate south of the Southern Boundary.     

Seasonal patterns in the fish and epibenthic crustaceans community of an intertidal zone with particular reference to the population dynamics of plaice and brown shrimp

The intertidal zone of a sandy beach located on the French coast of the Eastern Channel, was sampled during spring and summer 2000 to analyse the community structure of fish and epibenthic crustaceans. The presence of many juvenile fish (mainly O-group) and crustaceans indicated the important role played by the intertidal zone as a nursery ground. The brown shrimp, Crangon crangon and O-group plaice, Pleuronectes platessa are the two most abundant species of the intertidal ecosystem.Plaice settlement period extended from mid-March to early June. Over the survey period, densities increased to maximum numbers of about 27 ind. 10 m⁻² in mid-April. In the following week, density rapidly decreased due to mortality and migration into deeper waters. The mortality was attributed mainly to predation by brown shrimp (C. crangon) and to a lesser extent by the shore crab (Carcinus maenas). The mean size of 0-group plaice increased from 19 mm in mid-April to 58 mm in July. Growth of juvenile 0-group plaice is described by an exponential equation: total length (TL, mm) = 12.602 e^{0.022 (post-settlement age)}. Post-settlement growth rates, estimated by otolith microstructure analysis, were 0.38 mm d⁻¹ for plaice ≤30 mm and 0.61 mm d⁻¹ for plaice >30 mm. Settlement of juvenile brown shrimp started in mid-April, peaked in early June (93 ind. 10 m⁻²) and continued with fluctuating intensity throughout the summer. Growth rate of juvenile C. crangon, estimated after the settlement peak, was 0.163 mm d⁻¹. Growth conditions of juvenile plaice and C. crangon were analysed by comparing estimated growth in the field with predicted maximum growth according to temperature-growth rate models from experimental studies of growth with unlimited food supply. For plaice, the estimated growth rate was lower (plaice ≤30 mm) but similar (plaice >30 mm) to the predicted maximum growth suggesting a food limitation only for newly settled individual. The observed increase in mean length of juvenile C. crangon was lower than the maximum possible growth. The function of the intertidal zone in the early juvenile stages of marine species is discussed.     

Basin-scale variability of phytoplankton biomass,production and growth in the Atlantic Ocean

The latitudinal distributions of phytoplankton biomass, composition and production in the Atlantic Ocean were determined along a 10,000-km transect from 50°N to 50°S in October 1995, May 1996 and October 1996. Highest levels of euphotic layer-integrated chlorophyll a (Chl a) concentration (75–125 mg Chl m⁻²) were found in North Atlantic temperate waters and in the upwelling region off NW Africa, whereas typical Chl a concentrations in oligotrophic waters ranged from 20 to 40 mg Chl m⁻². The estimated concentration of surface phytoplankton carbon (C) biomass was 5–15 mg C m⁻² in the oligotrophic regions and increased over 40 mg C m⁻² in richer areas. The deep chlorophyll maximum did not seem to constitute a biomass or productivity maximum, but resulted mainly from an increase in the Chl a to C ratio and represented a relatively small contribution to total integrated productivity. Primary production rates varied from 50 mg C m⁻² d⁻¹ at the central gyres to 500–1000 mg C m⁻² d⁻¹ in upwelling and higher latitude regions, where faster growth rates (μ) of phytoplankton (>0.5 d⁻¹) were also measured. In oligotrophic waters, microalgal growth was consistently slow [surface μ averaged 0.21±0.02 d⁻¹ (mean±SE)], representing <20% of maximum expected growth. These results argue against the view that the subtropical gyres are characterized by high phytoplankton turnover rates. The latitudinal variations in μ were inversely correlated to the changes in the depth of the nitracline and positively correlated to those of the integrated nitrate concentration, supporting the case for the role of nutrients in controlling the large-scale distribution of phytoplankton growth rates. We observed a large degree of temporal variability in the phytoplankton dynamics in the oligotrophic regions: productivity and growth rates varied in excess of 8-fold, whereas microalgal biomass remained relatively constant. The observed spatial and temporal variability in the biomass specific rate of photosynthesis is at least three times larger than currently assumed in most satellite-based models of global productivity.     

Role of the Agulhas Current in Indian Ocean circulation and associated heat and freshwater fluxes

A reduced estimate of Agulhas Current transport provides the motivation to examine the sensitivity of Indian Ocean circulation and meridional heat transport to the strength of the western boundary current. The new transport estimate is 70 Sv, much smaller than the previous value of 85 Sv. Consideration of three case studies for a large, medium and small Agulhas Current transport demonstrate that the divergence of heat transport over the Indian Ocean north of 32°S has a sensitivity of 0.08 PW per 10 Sv of Agulhas transport, and freshwater convergence has a sensitivity of 0.03×10⁹ kg s⁻¹ per 10 Sv of transport. Moreover, a smaller Agulhas Current leads to a better silica balance and a smaller meridional overturning circulation for the Indian Ocean. The mean Agulhas Current transport estimated from time-series current meter measurements is used to constrain the geostrophic transport in the western boundary region in order to re-evaluate the circulation, heat and freshwater transports across 32°S. The Indonesian Throughflow is taken to be 12 Sv at an average temperature of 18°C. The constrained circulation exhibits a vertical–meridional circulation with a net northward flow below 2000 dbar of 10.1 Sv. The heat transport divergence is estimated to be 0.66 PW, the freshwater convergence to be 0.54×10⁹ kg s⁻¹, and the silica convergence to be 335 kmol s⁻¹. Meridional transports are separated into barotropic, baroclinic and horizontal components, with each component conserving mass. The barotropic component is strongly dependent on the estimated size of the Indonesian Throughflow. Surprisingly, the baroclinic component depends principally on the large-scale density distribution and is nearly invariant to the size of the overturning circulation. The horizontal heat and freshwater flux components are strongly influenced by the size of the Agulhas Current because it is warmer and saltier than the mid-ocean. The horizontal fluxes of heat and salt penetrate down to 1500 m depth, suggesting that warm and salty Red Sea Water may be involved in converting the intermediate and upper deep waters which enter the Indian Ocean from the Southern Ocean into warmer and saltier waters before they exit in the Agulhas Current.     

Zooplankton associated with the oxygen minimum zone system in the northern upwelling region of Chile during March 2000

Zooplankton in the coastal upwelling region off northern Chile may play a significant biogeochemical role by promoting carbon flux into the subsurface OMZ (oxygen minimum zone). This work identifies the dominant zooplankton species inhabiting the area influenced by the OMZ in March 2000 off Iquique (20°S, northern Chile). Abundance and vertical distribution studies revealed 17 copepod and 9 euphausiid species distributed between the surface and 600 m at four stations sampled both by day and by night. Some abundant species remained in the well-oxygenated upper layer (30 m), with no evidence of diel vertical migration, apparently restricted by a shallow (40–60 m) oxycline. Other species, however, were found closely associated with the OMZ. The large-sized copepod Eucalanus inermis was found below the oxycline and performed diel vertical migrations into the OMZ, whereas the very abundant Euphausia mucronata performed extensive diel vertical migrations between the surface waters and the core of the OMZ (200 m), even crossing it. A complete assessment of copepods and euphausiids revealed that the whole sampled water column (0–600 m) is occupied by distinct species having well-defined habitats, some of them within the OMZ. Ontogenetic migrations were evident in Eucalanidae and E. mucronata. Estimates of species biomass showed a substantial (>75% of total zooplankton biomass) daily exchange of C between the photic layer and the OMZ. Both E. inermis and E. mucronata can actively exchange about 37.8 g C m⁻² d⁻¹ between the upper well-oxygenated (0–60 m) layer and the deeper (60–600 m) OMZ layer. This migrant biomass may contribute about 7.2 g C m⁻² d⁻¹ to the OMZ system through respiration, mortality, and production of fecal pellets within the OMZ. This movement of zooplankton in and out of the OMZ, mainly as a result of the migratory behavior of E. mucronata, suggests a very efficient mechanism for introducing large amounts of freshly produced carbon into the OMZ system and should, therefore, be considered when establishing C budgets for coastal upwelling systems.     

Critical thermal maxima of common rocky intertidal fish and shrimps — A preliminary assessment

Rocky shore ecosystems are considered sentinels of climate warming because they are in close contact with the atmosphere and their shallow waters present low thermal inertia. Concerns on the vulnerability of rocky shore species subject to climate warming make the investigation of their thermal tolerance an urgent topic. The aim of this study was to determine the upper thermal limits of species that are common in tidal pools of rocky shore ecosystems of the Northeast Atlantic. The method used was the Critical Thermal Maximum (CTMax), which allowed the ranking of species in terms of their upper thermal limits as follows: Coryphoblennius galerita (32.0 °C), Palaemon serratus (33.0 °C), Gobius paganellus (33.1 °C), Palaemon elegans (33.4 °C), Lipophrys pholis (33.9 °C) and Paralipophrys trigloides (35.0 °C). Intraspecific variability was always lower than 2%.     

Oxygen distribution and aerobic respiration in the north and south eastern tropical Pacific oxygen minimum zones

Highly sensitive STOX O₂ sensors were used for determination of in situ O₂ distribution in the eastern tropical north and south Pacific oxygen minimum zones (ETN/SP OMZs), as well as for laboratory determination of O₂ uptake rates of water masses at various depths within these OMZs. Oxygen was generally below the detection limit (few nmol L⁻¹) in the core of both OMZs, suggesting the presence of vast volumes of functionally anoxic waters in the eastern Pacific Ocean. Oxygen was often not detectable in the deep secondary chlorophyll maximum found at some locations, but other secondary maxima contained up to ~0.4 µmol L⁻¹. Directly measured respiration rates were high in surface and subsurface oxic layers of the coastal waters, reaching values up to 85 nmol L⁻¹ O₂ h⁻¹. Substantially lower values were found at the depths of the upper oxycline, where values varied from 2 to 33 nmol L⁻¹ O₂ h⁻¹. Where secondary chlorophyll maxima were found the rates were higher than in the oxic water just above. Incubation times longer than 20 h, in the all-glass containers, resulted in highly increased respiration rates. Addition of amino acids to the water from the upper oxycline did not lead to a significant initial rise in respiration rate within the first 20 h, indicating that the measurement of respiration rates in oligotrophic Ocean water may not be severely affected by low levels of organic contamination during sampling. Our measurements indicate that aerobic metabolism proceeds efficiently at extremely low oxygen concentrations with apparent half-saturation concentrations (K_m values) ranging from about 10 to about 200 nmol L⁻¹.     

Seasonal changes in zooplankton abundance,biomass, size structure and dominant copepods in the Oyashio region analysed by an optical plankton counter

To identify seasonal patterns of change in zooplankton communities, an optical plankton counter (OPC) and microscopic analysis were utilised to characterise zooplankton samples collected from 0 to 150 m and 0 to 500 m in the Oyashio region every one to three months from 2002 to 2007. Based on the OPC measurements, the abundance and biomass of zooplankton peaked in June (0–150 m) or August (150–500 m), depending on the depth stratum. The peak periods of the copepod species that were dominant in terms of abundance and biomass indicated species-specific patterns. Three Neocalanus species (Neocalanus cristatus, Neocalanus flemingeri and Neocalanus plumchrus) exhibited abundance peaks that occurred before their biomass peaks, whereas Eucalanus bungii and Metridia pacifica experienced biomass peaks before their abundance peaks. The abundance peaks corresponded to the recruitment periods of early copepodid stages, whereas the biomass peaks corresponded to the periods when the dominant populations reached the late copepodid stages (C5 or C6). Because the reproduction of Neocalanus spp. occurred in the deep layer (>500 m), their biomass peaks were observed when the major populations reached stage C5 after the abundance peaks of the early copepodid stages. The reproduction of E. bungii and M. pacifica occurred near the surface layer. These species first formed biomass peaks of C6 and later developed abundance peaks of newly recruited early copepodid stages. From the comparison between OPC measurements and microscopic analyses, seasonal changes in zooplankton biomass at depths of 0–150 m were governed primarily by E. bungii and M. pacifica, whereas those at depths of 150–500 m were primarily caused by the three Neocalanus species.     

Spatial and temporal variability of planktonic archaeal abundance in the Humboldt Current System off Chile

The latest advances in the field of microbial ecology have shown that planktonic Archaea are one of the most abundant unicellular microorganisms of the oceans. However, no information is available on the contribution this group makes to the prokaryote assemblages that inhabit the eastern South Pacific Ocean. Here, we describe the relative abundance and vertical distribution of planktonic Archaea off northern and central-southern Chile. Data come from several cruises and a 45-month time series at a station located on the shelf off central-southern Chile. Both the taxonomic composition of the prokaryote community and its relative abundance were determined using quantitative dot blot 16S-rRNA hybridizations. Total Archaea in central-southern Chile made up 6–87% of the prokaryote rRNA in the water column and did not present evidence of any seasonal pattern. Crenarchaea were the most abundant archaeal group at this site and were significantly associated with the ammonium concentration (r²=0.16, p=0.0003, n=80). Archaeal abundance in the time series was usually greater in the deeper layer (>50 m), with contributions reaching up to ∼90% of the prokaryote rRNA on certain occasions, and decreasing towards the surface. Important increments in the relative abundance of total Archaea were observed on given dates at the surface of the time-series station off central-southern Chile. Off northern Chile, total Archaea normally contributed from ∼10% to 50% of the prokaryote rRNA found between 10 and 1000 m, and were generally important in the mesopelagic realm. Our results indicate that Archaea constitute an important fraction of the prokaryote assemblage in the water column of the Humboldt Current System, especially in the oxygen minimum zone.     

The influence of the south-west monsoon upon the nutrient biogeochemistry of the Arabian Sea

Variations in the nutrient concentrations were studied during two cruises to the Arabian Sea. The situation towards the end of the southwest monsoon season (September/October 1994) was compared with the inter-monsoonal season during November and December 1994. Underway surface transects showed the influence of an upwelling system during the first cruise with deep, colder, nutrient-rich water being advected into the surface mixed layer. During the southwesterly monsoon there was an area of coastal Ekman upwelling, bringing colder water (24.2°C) into the surface waters of the coastal margin. Further offshore at about 350 km there was an area of Ekman upwelling, as a result of wind-stress curl, north of the Findlater Jet axis; this area also had cooler surface water (24.6°C). Further offshore (>1000 km) the average surface temperatures increased to >27°C. These waters were oligotrophic with no evidence of the upwelling effects observed further inshore. In the upwelling regions nutrient concentrations in the close inshore coastal zone were elevated (NO₃=18 μmol l^-1, PO₄=1.48 μmol l^-1); higher concentrations also were measured at the region of offshore upwelling off the shelf, with a maximum nitrate concentration of 12.5 μmol l^-1 and a maximum phosphate concentration of 1.2 μmol l^-1. Nitrate and phosphate concentrations decreased with increasing distance offshore to the oligotrophic waters beyond 1400 km, where typical nitrate concentrations were 35.0 nmol l^-1 (0.035 μmol l^-1) in the surface mixed layer. A CTD section from the coastal shelf, to 1650 km offshore to the oligotrophic waters, clearly showed that during the monsoon season, upwelling is one of the major influences upon the nutrient concentrations in the surface waters of the Arabian Sea off the coast of Oman. Productivity of the water column was enhanced to a distance of over 800 km offshore. During the intermonsoon period a stable surface mixed layer was established, with a well-defined thermocline and nitracline. Surface temperature was between 26.8 and 27.4°C for the entire transect from the coast to 1650 km offshore. Nitrate concentrations were typically between 2.0 and 0.4 μmol l^-1 for the transect, to about 1200 km where the waters became oligotrophic, and nitrate concentrations were then typically 8–12 nmol l^-1. Ammonia concentrations for the oligotrophic waters were typically 130 nmol l^-1, and are reported for the first time in the Indian Ocean. The nitrogen/phosphorus (N/P) ratios suggest that phytoplankton production was potentially nitrogen-limited in all the surface waters of the Arabian Sea, with the greatest nitrogen limitation during the intermonsoon period.     

DOC dynamics in the meso and bathypelagic layers of the Mediterranean Sea

Seven years (2001–2008) of dissolved organic carbon (DOC) vertical profiles were examined in order to assess the main processes determining DOC concentration and distribution in the meso- and bathypelagic layers of the Mediterranean Sea. As expected, DOC showed high and highly variable concentrations in the surface layer of 57–68 μM (average values between 0 and 100 m), with a decrease to 44–53 μM between 200 and 500 m. Deep DOC distribution was strongly affected by deep-water formation, with a significant increase to values of 76 μM in recently ventilated deep waters, and low concentrations, comparable to those observed in the open oceanic waters (34–45 μM), where the oldest, deep waters occurred. In winter 2004/2005 a deep-water formation event was observed and the consequent DOC export at depth was estimated to range between 0.76–3.02 Tg C month^–1. In the intermediate layer, the main path of the Levantine Intermediate Water (LIW) was followed in order to estimate the DOC consumption rate in its core. Multiple regression between DOC, apparent oxygen utilization (AOU), and salinity indicated that 38% of the oxygen consumption was related to DOC mineralization when the effect of mixing was removed. In deep waters of the southern Adriatic Sea a DOC decrease of 6 μM, together with an AOU increase of 9 μM, was observed between the end of January 2008 and the end of June 2008 (5 months). These data indicate a rate of microbial utilization of DOC of about 1.2 μM C month⁻¹, with 92% of the oxygen consumption due to DOC mineralization. These values are surprisingly high for the deep sea and represent a peculiarity of the Mediterranean Sea.     

Microzooplankton composition,biomass and grazing rates along the WOCE SR3 line between Tasmania and Antarctica

Microzooplankton species composition and grazing rates on phytoplankton were investigated along a transect between ∼46 and 67°S, and between 140 and 145°E. Experiments were conducted in summer between November 2nd and December 14th in 2001. The structure of the microbial food web changed considerably along the transect and was associated with marked differences in the physical and chemical environment encountered in the different water masses and frontal regions. On average microzooplankton grazing experiments indicated that 91%, 102%, and 157%, (see results) of the phytoplankton production would be grazed in the <200, <20 and <2 μm size fractions, respectively, indicating microzooplankton grazing was potentially constraining phytoplankton populations (<200 μm) along most of the transect. Small ciliates in general and especially oligotrich species declined in importance from the relatively warm, Southern Subtropical Front waters (6.8 μg C/L) to the colder waters of the southern branch of the Polar Front (S-PF), (∼0.5 μg C/L) before increasing again near the Antarctic landmass. Large changes in microzooplankton dominance were observed, with heterotrophic nanoflagellates (HNF), ciliates and larger dinoflagellates having significant biomass in different water masses. HNF were the dominant grazers when chlorophyll a was low in areas such as the Inter-Polar Frontal Zone (IPFZ), while in areas of elevated biomass such as the S-PF and Southern Antarctic Circumpolar Current (SACC), a mix of copepod nauplii and large heterotrophic and mixotrophic dinoflagellates tended to dominate the grazing community. In the S-PF and SACC water masses the tight coupling observed between the microzooplankton grazers and phytoplankton populations over most of the rest of the transect was relaxed. In these regions grazing was low on the >20 μm size fraction of chlorophyll a, which dominated the biomass, while smaller diatoms and nanoplankton in the <20 μm size fraction were still heavily grazed. The lack of grazing pressure on large phytoplankton contributes to this region's potential to export carbon with larger cells known to have higher sinking rates.     

Persian Gulf response to a wintertime shamal wind event

The results from a～1 km resolution HYbrid Coordinate Ocean Model (HYCOM), forced by 1/2° Navy Operational Global Atmospheric Prediction System (NOGAPS) atmospheric data, were used in order to study the dynamic response of the Persian Gulf to wintertime shamal forcing. Shamal winds are strong northwesterly winds that occur in the Persian Gulf area behind southeast moving cold fronts. The period from 20 November to 5 December 2004 included a well defined shamal event that lasted 4–5 days. In addition to strong winds (16 m s^?1) the winter shamal also brought cold dry air (T_a=20 °C, q_a=10 g kg^?1) which led to a net heat loss in excess of 1000 W m^?2 by increasing the latent heat flux. This resulted in SST cooling of up to 10 °C most notably in the northern and shallower shelf regions. A sensitivity experiment with a constant specific humidity of q_a=15 g kg^?1 confirmed that about 38% of net heat loss was due to the air–sea humidity differences. The time integral of SST cooling closely followed the air–sea heat loss, indicating an approximate one-dimensional vertical heat balance. It was found that the shamal induced convective vertical mixing provided a direct mechanism for the erosion of stratification and deepening of the mixed layer by 30 m. The strong wind not only strengthened the circulation in the entire Persian Gulf but also established a northwestward flowing Iranian Coastal Current (ICC, 25–30 cm s^?1) from the Strait of Hormuz to about 52°E, where it veered offshore. The strongest negative sea level of 25–40 cm was generated in the northernmost portion of the Gulf while the wind setup against the coast of the United Arab Emirates established a positive sea level of 15–30 cm. The transport through the Strait of Hormuz at 56.2°E indicated an enhanced outflow of 0.25 Sv (Sv≡10⁶ m³ s^?1) during 24 November followed by an equivalent inflow on the next day.     

Latitudinal distribution of microbial plankton abundance,production, and respiration in the Equatorial Atlantic in autumn 2000

Phytoplankton and bacterial abundance, size-fractionated phytoplankton chlorophyll-a (Chl-a) and production together with bacterial production, microbial oxygen production and respiration rates were measured along a transect that crossed the Equatorial Atlantic Ocean (10°N–10°S) in September 2000, as part of the Atlantic Meridional Transect 11 (AMT 11) cruise. From 2°N to 5°S, the equatorial divergence resulted in a shallowing of the pycnocline and the presence of relatively high nitrate (>1 μM) concentrations in surface waters. In contrast, a typical tropical structure (TTS) was found near the ends of the transect. Photic zone integrated ¹⁴C primary production ranged from ∼200 mg C m⁻² d⁻¹ in the TTS region to ∼1300 mg C m⁻² d⁻¹ in the equatorial divergence area. In spite of the relatively high primary production rates measured in the equatorial upwelling region, only a moderate rise in phytoplankton biomass was observed as compared to nearby nutrient-depleted areas (22 vs. 18 mg Chl-a m⁻², respectively). Picophytoplankton were the main contributors (>60%) to both Chl-a biomass and primary production throughout the region. The equatorial upwelling did not alter the phytoplankton size structure typically found in the tropical open ocean, which suggests a strong top-down control of primary producers by zooplankton. However, the impact of nutrient supply on net microbial community metabolism, integrated over the euphotic layer, was evidenced by an average net microbial community production within the equatorial divergence (1130 mg C m⁻² d⁻¹) three-fold larger than net production measured in the TTS region (370 mg C m⁻² d⁻¹). The entire region under study showed net autotrophic community metabolism, since respiration accounted on average for 51% of gross primary production integrated over the euphotic layer.     

Distributions and fluxes of methyl chloride and methyl bromide in the East China Sea and the Southern Yellow Sea in autumn

We determined the distributions and fluxes of methyl chloride and methyl bromide in the East China Sea (ECS) and the Southern Yellow Sea (SYS) in November 2007. Methyl chloride and methyl bromide concentrations in the surface waters ranged from 47.1 to 163 pmol L^?1 and from 0.70 to 9.82 pmol L^? 1, with average values of 87.6 and 2.97 pmol L^? 1, respectively. The distributions of the two methyl halides were clearly influenced by the Yangtze (Changjiang) River effluent and Kuroshio water, with high concentrations appearing in the coastal zone and low values occurring in the open waters. A positive linear correlation was observed between methyl chloride and methyl bromide concentration anomalies in the surface waters, suggesting that they may share some origins in this coastal area. However, no correlation was found between the two methyl halide concentration anomalies and chlorophyll a in the surface waters. The vertical profiles of the two methyl halides were characterized by the maxima in the upper mixed layer. Both gases were generally supersaturated in the surface seawater, with mean sea-to-air fluxes of methyl chloride and methyl bromide of 391 and 20.0 nmol m^?2 d^? 1, respectively.     

Physical-biological coupling in the Algerian Basin (SW Mediterranean): Influence of mesoscale instabilities on the biomass and production of phytoplankton and bacterioplankton

The biomass and production of phytoplankton and bacterioplankton was investigated in relation to the mesoscale structures found in the Algerian Current during the ALGERS'96 cruise (October 1996). Biological determinations were carried out in three transects between 0° and 2°E aimed at crossing a so-called event, formed by a coastal anticyclonic eddy associated with an offshore cyclonic eddy to the west. The concentration of chlorophyll a (Chl) was maximum (>1.2 mg m⁻³) within the cyclonic eddy and at the frontal zones between the Modified Atlantic Water (MAW) of the Algerian Current and the Mediterranean waters further north. Chl (total and >2 μm) was significantly correlated with proxies of nutrient flux into the upper layers. Autotrophic picoplankton and heterotrophic bacterial abundance and production presented clear differences between MAW and Mediterranean water, with higher values at those stations under the influence of the Algerian Current. In general, greater differences were observed in production than in biomass variables. The photosynthetic parameters (derived from P–E relationships) and integrated primary production (range 189–645 mg m⁻² d⁻¹) responded greatly to the different hydrological conditions. The mesoscale phenomena inducing fertilization caused a 2 to 3-fold increase in primary production rates. The relatively high values found within the cyclonic eddy suggest that, although short-lived in comparison with anticyclonic eddies, these eddies may produce episodic increases of biological production not accounted for in previous surveys in the region.