A method for the selective removal of inorganic ash particles from lake sediments

Inorganic ash particles are formed by the fusing of inorganic material present during the high temperature combustion of fossil fuels. As they accumulate in lake sediments, they record the history of atmospheric contamination produced from such sources. A technique has been developed for concentrating these particles from lake sediments involving the stepwise removal of unwanted components of the sediment, including organic material and biogenic silica.When applied to a sediment core taken from Loch Tinker, central Scotland, a particle concentration profile, very similar to that of the carbonaceous particle profile (the other component of fossil-fuel combustion ash) is produced. The concentration of the inorganic ash spheres in the sediment is approximately an order of magnitude higher than the carbonaceous particles and there appears to be a continuous pre-industrial background value. This seems to imply a similar source (i.e. coal combustion rather than oil) for both inorganic ash and carbonaceous particles. This method has also been used with success on peat cores.     

Impact of pre-Columbian agriculture, climate change, and tectonic activity inferred from a 5,700-year paleolimnological record from Lake Nicaragua

Lake Nicaragua, the largest lake in Central America, is a promising site for paleolimnological study of past climate change, tectonic and volcanic activity, and pre-Columbian agriculture in the region. It is near the northern limit of the Intertropical Convergence Zone (ITCZ), which brings the rainy season to the tropics, so effects of decreasing precipitation due to southern migration of the ITCZ through the Holocene should be observable. Because fault zones and an active volcano lie within the lake, the long-term impact of tectonic and volcanic activity can also be examined. Finally, the fertile volcanic soils near the lake may have encouraged early agriculture. We analyzed diatoms, biogenic silica (BSi), total organic carbon (TOC), water content, volcanic glass, and magnetic susceptibility in a sediment core from Lake Nicaragua with eleven accelerator mass spectroscopy radiocarbon dates, spanning ~5,700 years. Sediment accumulation rates decreased from the bottom to the top of the core, indicating a general drying trend through the Holocene. An increase in eutrophic diatom abundance suggests that pre-Columbian agriculture impacted the lake as early as ~5,400 cal yr BP. Above a horizon of coarser grains deposited sometime between ~5,200 and 1,600 cal yr BP, planktonic diatoms increased and remained dominant to the top of the core, indicating that water depth permanently increased. Although magnetic susceptibility peaked and water content dipped at the coarse horizon, volcanic glass fragments did not increase, suggesting that the coarse horizon and subsequent increase in water depth were caused by tectonic rather than by volcanic activity. Decreased accumulation rates of BSi and TOC indicate that water became clearer when depth increased.     

Late Pleistocene and Holocene history of Lake Terrasovoje,Amery Oasis,East Antarctica,and its climatic and environmental implications

A 5.52 m long sediment sequence was recovered from Lake Terrasovoje, Amery Oasis, East Antarctica, in order to reconstruct the regional environmental history. The basal sediments, which are dominated by glacial and glaciofluvial clastic sediments, attest to a Late Pleistocene deglaciation of the lake basin. These sediments are overlain by 2.70 m of laminated algal and microbial mats and a few interspersed moss layers. Radiocarbon dating, conducted on bulk organic carbon of 12 samples throughout the organic sequence, provides a reliable chronology since the onset of biogenic accumulation at c. 12,400 cal. year BP. Successful diatom colonization, however, was probably hampered by extensive ice and snow cover on the lake and restricted input of nutrients until 10,200 cal. year BP. A subsequent increase of nutrient supply culminated between 8600 and 8200 cal. year BP and is related to warm summer temperatures and reduced albedo in the catchment. Warm conditions lasted until 6700 cal. year BP, supporting the establishment of a diatom community. Colder temperatures from 6700 cal. year BP culminated in several periods between 6200 and 3700 cal. year BP, when high amounts of sulphur and low abundances of diatoms were deposited due to a perennial ice and snow cover on the lake. During the late Holocene, relatively warm conditions between 3200 and 2300 cal. year BP and between 1500 to 1000 cal. year BP, respectively, indicated by high accumulation of organic matter and reducing bottom water conditions, were interrupted and followed by colder periods.     

南极布兰斯菲尔德海峡的硅质沉积物特征

根据粒度、微体生物组合、矿物组分和微量元素丰度,探讨了南极布兰斯菲尔德海峡中央海槽和南部下陆坡区冰－海沉积环境形成的硅质沉积物特征。硅质沉积物主要为硅藻软泥或粉砂质硅藻软泥,由微体生物遗体、粘土矿物、火山物质和陆源碎屑矿物所组成。硅质生物壳体主要为硅藻,含量＞３０％,含少量放射虫和硅质壳有孔虫。粘土矿物中蒙脱石的含量高达５２．５％,其次为陆源伊利石,高岭石和绿泥石含量相对较低。碎屑重矿物以橄榄石、辉石、角闪石等非稳定组合为特征,轻矿物主要包括石英、长石和火山玻璃。微量元素丰度特征与大洋现代表层沉积物有所区别。研究区硅质沉积作用具有多种物源、生物沉积作用居重要地位、陆源（包括火山）物质以粉砂和粘土级细质点为主、相对较高的沉积速率和受地质构造背景影响等特征。     

Variability in diatom and chrysophyte assemblages and inferred pH: paleolimnological studies of Big Moose Lake,New York,USA

We measured variability in the composition of diatom and chrysophyte assemblages, and the pH inferred from these assemblages, in sediment samples from Big Moose Lake, in the Adirondack Mountains of New York. Replicate samples were analyzed from (1) a single sediment core interval, (2) 12 different intervals from each of 3 separate cores, and (3) 10 widely spaced surface sediment samples (0–1 cm). The variability associated with sample preparation (subsampling, processing, and counting) was relatively small compared to between-core and within-lake variability. The relative abundances of the dominant diatom taxa varied to a greater extent than those of the chrysophyte scale assemblages. Standard deviations of pH inferences for multiple counts from the same sediment interval from diatom, chrysophyte, and diatom plus chrysophyte inference equations were 0.04 (n=8), 0.06 (n=32), and 0.06 (n=8) of a pH unit, respectively. Stratigraphic analysis of diatoms and chrysophytes from three widely spaced pelagic sediment cores provided a similar record of lake acidification trends, although with slight differences in temporal rates of change. Average standard deviations of pH inferences from diatom, chrysophyte and diatom plus chrysophyte inference equations for eight sediment intervals representing similar time periods but in different cores were 0.10, 0.20, and 0.09 pH unit, respectively. Our data support the assumption that a single sediment core can provide an accurate representation of historical change in a lake. The major sources of diatom variability in the surface sediments (i.e., top 1.0 cm) were (1) differences in diatom assemblage contributions from benthic and littoral sources, and (2) the rapid change in assemblage composition with sediment depth, which is characteristic of recently acidified lakes. Because scaled chrysophytes are exclusively planktonic, their spatial distribution in lake sediments is less variable than the diatom assemblages. Standard deviations of pH inferences for 10 widely spaced surface sediment samples from diatom, chrysophyte and diatom plus chrysophyte inference equations were 0.21, 0.09, and 0.16 of a pH unit, respectively.     

Strong Magnetic Levels in Lake Chapala Sediments (western Mexico): Their Mineralogy and Stratigraphic Significance

Lake Chapala, located 120 km northeast of Colima Volcano, lies at the north and northeast of the Citala rift in the Trans-Mexican Volcanic Belt. It belongs to the watershed of the Lerma River, which originates from the Mexico City area, 450 km to the east. Sediment cores, 0.5–2 m long, were collected from the lake. Magnetic susceptibility of the lake sediments generally ranges from 400 to 800 × 10⁻⁹ m³ kg⁻¹; but in some layers it exceeds 1000 or 1500 × 10⁻⁹ m³ kg⁻¹. The magnetic susceptibility vertical profiles display a thin peak (2–5 cm) or a double peak with magnetic susceptibility > 2000 × 10⁻⁹ m³ kg⁻¹. Scanning electron microscope analysis shows that the main mineral responsible for the magnetic susceptibility is titano-magnetite, which is relatively abundant in the magnetic layers. In most of the cores, the layer with magnetic susceptibility > 2000 × 10⁻⁹ m³ kg⁻¹ is coarser grained than the directly under and overlying sediments, which are composed of fine silt. But not all coarser levels are magnetic nor are all magnetic levels bound to coarser layers. The high titano-magnetic concentration probably originates from volcanic ash falls. Sedimentation rates, evaluated for several sites by means of the ²¹⁰Pb and ¹³⁷Cs methods, allow a date approximation (between 1535 and 1670) for the strong magnetic layer deposition. Since important eruptions of Colima Volcano, with ash fall, were reported from 1576 to 1623, the main peak of magnetic susceptibility in the sediment profiles is assumed to be related to these events. The main magnetic layer extends to greater depth in the profiles of the eastern part of the lake plain than at the west. It can, therefore, be suggested that a relative subsidence occurs in the eastern part of the lake; the axis of the eastern plain overlaps the area displaying the maximum subsidence rate and seems therefore to correspond to the prolongation of Citala rift.     

Tracking recent recovery from eutrophication in a high arctic lake (Meretta Lake, Cornwallis Island, Nunavut, Canada) using fossil diatom assemblages

Meretta Lake (Resolute Bay, Cornwallis Island, Nunavut, Canada) is a high arctic lake that received raw sewage for almost 50 years from the Canadian Department of Transport Base. The lake was sampled from 1968–72 during the International Biological Programme, as part of the Char Lake Project. As the number of users at the Transport Base declined throughout the 1990s, so too did the lake's nutrient levels, and Meretta Lake is now classified as oligotrophic. A previous diatom-based paleolimnological study revealed marked species assemblage shifts coincident with sewage inputs beginning in the late 1940s; however, because the core was taken at a time when nutrient levels were still relatively high (i.e., 1993), the diatom record did not yet track any signs of recovery. In this present study, we examined fossil diatom assemblages from a sediment core taken in 2001. Our results indicate a shift to the pre-impact diatom assemblages in the most recent sediments, indicating that the paleolimnological record is tracking the decreased nutrient inputs to this high arctic lake, and confirms that no significant lags exist in these largely ice-covered lakes.     

The CASPIA project: diatoms,salt lakes,and environmental change

The Climate and Salinity (CASPIA) Project is concerned with diatoms as environmental indicators in inland waters and their use in reconstructing salinity and major ion composition from fossil diatom assemblages in lake sediments. By comparing saline lake diatom floras from around the world the project aims to establish a common, harmonised approach to sample collection, diatom identification and nomenclature, and to develop techniques for numerical analysis and data storage.This is the first in a series of papers published in this issue on the paleolimnology of arid regions. These papers were presented at the Sixth International Palaeolimnology Symposium held 19–21 April, 1993 at the Australian National University, Canberra, Australia. Dr A. R. Chivas served as guest editor for these papers.Palliser Triangle Global Change Project Contribution Number 19.     

Late Quaternary-Holocene lake-level changes in the eastern margin of the Thar Desert,India

A study on two closed salt lake basins, Tal Chapar and Parihara in the eastern margin of the Thar Desert, Rajasthan, was carried out to unravel late Quaternary geomorphic evolution of these saline lakes. Both lakes are elliptical in shape bordered by stabilised dunes, and are oriented in a NE-SW direction, i.e., in the direction of the prevailing summer monsoon wind. Both lakes have been formed in the wind-shadow zones of isolated hills of Precambrian quartzite. Our study indicates that the late Quaternary sediments in the lakes began with the cyclic deposition of laminated fine silt layers (0.5 m thick), rich in organic matter, alternating with ripple cross-bedded sand layers (each ∼1.5–2 m thick). Sand layers that are moderately sorted are separated by laminated silt-clay layers with gypsum/calcite and this unit occurs in the upper most 4 m sequence in deeper sections. The presence of gypsum crystals within the laminated sediments suggests a high concentration of Ca in the inflowing water. At Parihara Lake the organic carbon-rich sediments at 95 cm depth was dated to 7,375 + 155/−150 year BP. At Tal Chapar radiocarbon dates of 7,190 + 155/−150 and 9,903 + 360/−350 was obtained from the sediments rich in organic carbon occurring at a depth of 1.35 m and 1.80 m, respectively. The study reveals strong hydrologic oscillations during the past ∼14,000 year BP (13,090 + 310/−300 year BP). Quaternary geomorphic processes, especially the strong aeolian processes during dry climatic phases, played a major role in the formation of the lake basins, as well as the fringing linear dunes. Geochemical and mineralogical analyses of the lacustrine sediments, supported by radiocarbon dates indicate the existence of an ephemeral lake earlier than ∼13,000 year BP as sediments began to be deposited in a lacustrine environment implying sustained runoff in the catchments. A freshwater lake formed between 9,000 year and 7,000 year BP. The lake dried periodically and this strong fluctuating regime continued until about ∼7,000 year BP. Mid-Holocene was wet and this was possibly due to higher winter rains A saline lake existed between 6,000 year and 1,300 year BP and finally present day semi arid conditions set in since 1,200 year BP. Remnants of a habitation site (hearth and charred bones) on stabilised dune at Devani near Tal Chapar were dated to 240 ± 120 year, while that at Gopalpura was dated to 335 ± 90 year. These historical sites on stabilised dunes were, according to the local accounts, settlements of people who used the lake brine for manufacturing salt.     

Diatoms in sediments of perennially ice-covered Lake Hoare, and implications for interpreting lake history in the McMurdo Dry Valleys of Antarctica

Diatom assemblages in surficial sediments, sediment cores, sediment traps, and inflowing streams of perennially ice-covered Lake Hoare, South Victorialand, Antarctica were examined to determine the distribution of diatom taxa, and to ascertain if diatom species composition has changed over time. Lake Hoare is a closed-basin lake with an area of 1.8 km², maximum depth of 34 m, and mean depth of 14 m, although lake level has been rising at a rate of 0.09 m yr^-1 in recent decades. The lake has an unusual regime of sediment deposition: coarse grained sediments accumulate on the ice surface and are deposited episodically on the lake bottom. Benthic microbial mats are covered in situ by the coarse episodic deposits, and the new surfaces are recolonized. Ice cover prevents wind-induced mixing, creating the unique depositional environment in which sediment cores record the history of a particular site, rather than a lake-wide integration. Shallow-water (<1 m) diatom assemblages (Stauroneis anceps, Navicula molesta, Diadesmis contenta var. parallela, Navicula peraustralis) were distinct from mid-depth (4–16 m) assemblages (Diadesmis contenta, Luticola muticopsis fo. reducta, Stauroneis anceps, Diadesmis contenta var. parallela, Luticola murrayi) and deep-water (26–31 m) assemblages (Luticola murrayi, Luticola muticopsis fo. reducta, Navicula molesta). Analysis of a sediment core (30 cm long, from 11 m water depth) from Lake Hoare revealed two abrupt changes in diatom assemblages. The upper section of the sediment core contained the greatest biomass of benthic microbial mat, as well as the greatest total abundance and diversity of diatoms. Relative abundances of diatoms in this section are similar to the surficial samples from mid-depths. An intermediate zone contained less organic material and lower densities of diatoms. The bottom section of core contained the least amount of microbial mat and organic material, and the lowest density of diatoms. The dominant process influencing species composition and abundance of diatom assemblages in the benthic microbial mats is episodic deposition of coarse sediment from the ice surface.     

GIS‐Based Indices for Comparing Airline Flight‐Path Vulnerability to Volcanoes

Volcanic ash can cause physical damage to an aircraft to the point of mechanical and electrical malfunction. In the event of an ash plume, aircraft would likely be diverted or grounded, leading to economic losses. This article presents two indices to compare the economic vulnerability of flight paths to volcanic ash plumes. The first index includes three factors for a flight path: wind direction, distance to the volcano, and flight‐path attributes (i.e., number of flights per day, number of potential passengers, and average ticket cost). The second index compares the threat posed by different volcanoes to an airline flight‐path network. A case study is presented for two airlines in the northwest United States to a Mt. Adams eruption. The results show that although the first airline has more flight paths in the region, the second airline is slightly more vulnerable due to the spatial relationships of its flight paths to the volcano and their attributes.     

Sedimentary DNA versus morphology in the analysis of diatom-environment relationships

The Arctic treeline ecotone is characterised by a steep vegetation gradient from arctic tundra to northern taiga forests, which is thought to influence the water chemistry of thermokarst lakes in this region. Environmentally sensitive diatoms respond to such ecological changes in terms of variation in diatom diversity and richness, which so far has only been documented by microscopic surveys. We applied next-generation sequencing to analyse the diatom composition of lake sediment DNA extracted from 32 lakes across the treeline in the Khatanga region, Siberia, using a short fragment of the rbcL chloroplast gene as a genetic barcode. We compared diatom richness and diversity obtained from the genetic approach with diatom counts from traditional microscopic analysis. Both datasets were employed to investigate diversity and relationships with environmental variables, using ordination methods. After effective filtering of the raw data, the two methods gave similar results for diatom richness and composition at the genus level (DNA 12 taxa; morphology 19 taxa), even though there was a much higher absolute number of sequences obtained per genetic sample (median 50,278), compared with microscopic counts (median 426). Dissolved organic carbon explained the highest percentage of variance in both datasets (14.2 % DNA; 18.7 % morphology), reflecting the compositional turnover of diatom assemblages along the tundra-taiga transition. Differences between the two approaches are mostly a consequence of the filtering process of genetic data and limitations of genetic references in the database, which restricted the determination of genetically identified sequence types to the genus level. The morphological approach, however, allowed identifications mostly to species level, which permits better ecological interpretation of the diatom data. Nevertheless, because of a rapidly increasing reference database, the genetic approach with sediment DNA will, in the future, enable reliable investigations of diatom composition from lake sediments that will have potential applications in both paleoecology and environmental monitoring.     

Advanced Micro-XRF Method to Separate Sedimentary Rhythms and Event Layers in Sediments: Its Application to Lacustrine Sediment from Lake Suigetsu,Japan

Event-related sedimentary layers, which are deposited occasionally due to volcanic eruptions, earthquakes or heavy rains, are often contained in the rhythmical sequences of lacustrine and marine sediments. We have developed an analytical method for separating the sedimentary rhythms and the event layers identified using the scanning X-ray analytical microscope (SXAM) and obtained sequential profiles of seven elements Al, Si, K, Ca, Ti, Mn, and Fe in the lacustrine sediment from Lake Suigetsu, Japan. Two types of event layers could be detected from the elemental composition of 33 layers of sediment: three known volcanic ash layers and 30 clay layers containing 12 turbidites. The recurrence interval of the latter, which may potentially be initiated and archived by locally important earthquakes, is estimated to be an average of 640 ± 160 years by using Sompi event analysis (SEA) based on an autoregressive (AR) model. After removing those portions that represented event layers from the elemental profiles, we obtained event-removed (ER) temporal profiles based on the tephrochronology of the three volcanic ash layers. The ER temporal profiles of manganese and iron, probably representing the siderite content, showed a millennial-scale variation in the Holocene that corresponded well with ice-rafting events in the North Atlantic.     

Functional attributes of epilithic diatoms for palaeoenvironmental interpretations in South-West Greenland lakes

Benthic diatoms are commonly used for palaeoenvironmental reconstruction in Arctic regions, but interpretation of their ecology remains challenging. We studied epilithic diatom assemblages from the shallow margins of 19 lakes from three areas (coast-inland-ice sheet margin) along a climate gradient in Kangerlussuaq, West Greenland during two periods; shortly after ice-off (spring) and in the middle of the growth season (summer). We aimed to understand the distribution of Arctic epilithic diatoms in relation to water chemistry gradients during the two seasons, to investigate their incorporation into lake sediments and to assess their applicability as palaeoenvironmental indicators. Diatoms were correlated with nutrients in the spring and alkalinity/major ions in the summer, when nutrients were depleted; approximately half of the variance explained was independent of spatial factors. When categorised by functional attributes, diatom seasonal succession differed among regions with the most obvious changes in inland lakes where summer temperatures are warmer, organic nutrient processing is prevalent and silicate is limiting. These conditions led to small, motile and adnate diatoms being abundant in inland lakes during the summer (Nitzschia spp., Encyonopsis microcephala), as these functional attributes are suited to living within complex mats of non-siliceous microbial biofilms. Seasonal succession in silica-rich lakes at the coast was less pronounced and assemblages included Tabellaria flocculosa (indicating more acidic conditions) and Hannaea arcus (indicating input from inflowing rivers). The nitrogen-fixing diatom Epithemia sorex increased from the coast to the ice sheet, negatively correlating with a gradient of reactive nitrogen. The presence of this diatom in Holocene sediment records alongside cyanobacterial carotenoids during arid periods of low nitrogen delivery, suggests that it is a useful indicator of nitrogen limitation. Nitzschia species appear to be associated with high concentrations of organic carbon and heterotrophy, but their poor representation in West Greenland lake sediments due to taphonomic processes limits their palaeoenvironmental application in this region. Proportions of epilithic taxa in lake sediment records of coastal lakes increased during some wetter periods of the Holocene, suggesting that snowpack-derived nutrient delivery may offer diatom taxa living at lake margins a competitive advantage over planktonic diatoms during the “moating” ice melt period. Thus, further research investigating linkages between epilithic diatoms, snowpack and nutrient delivery in seasonally frozen lakes is recommended as these taxa live on the ‘front-line’ during the spring and may be especially sensitive to changes in snowmelt conditions.     

The influence of Holocene tree-line advance and retreat on an arctic lake ecosystem: a multi-proxy study from Kharinei Lake,North Eastern European Russia

A consequence of predicted climate warming will be tree-line advance over large areas of the Russian tundra. Palaeolimnological techniques can be used to provide analogues of how such changes in tree-line advance and subsequent retreat affected lake ecosystems in the past. A Holocene sediment core taken from Kharinei Lake (Russia) was dated radiometrically and used for multi-proxy analyses with the aim of determining how climate and tree-line dynamics affected the productivity, community structure, carbon cycling and light regime in the lake. Pollen and macrofossil analyses were used to determine the dates of the arrival and retreat of birch and spruce forest. C:N ratios and percent loss-on-ignition were used to infer past changes in sediment organic matter. Visible-near-infrared spectroscopy and diatom analysis were used to infer past changes in lake-water carbon. Algal pigments and aquatic macrophytes were used to determine changes in lake productivity and light. Chironomids together with remains of the aquatic flora and fauna were used to provide information on past July temperature and continentality. Lake sedimentation was initiated shortly before 11,000 cal. years BP, when both chironomid- and pollen-inferred temperature reconstructions suggest higher summer temperatures than present, between 1 and 2°C warmer, and lake productivity was relatively high. A few trees were already present at this time. The spruce forest expanded at 8,000 cal. year BP remaining in the vicinity of the lake until 3,500 cal. year BP. This period coincided with a high concentration of organic material in the water column, and relatively high benthic productivity, as indicated by a high benthic: planktonic diatom ratio. After tree-line retreat, the optical transparency of the lake increased, and it became more open and exposed, and was thus subject to greater water-column mixing resulting in a higher abundance of diatom phytoplankton, especially heavily silicified Aulocoseira species. The colder climate resulted in a shorter ice-free period, the lake was less productive and there was a loss of aquatic macrophytes. Increased wind-induced mixing following forest retreat had a greater influence on the lake ecosystem than the effects of decreasing organic matter concentration and increased light penetration.     

Linking neo- and palaeolimnology: a case study using crater lake diatoms from central Turkey

Palaeoenvironmental interpretations from analyses of lake sediment archives are dependent on our understanding of the modern lake environment and the link between neo- and palaeolimnology. Diatom assemblages represent different lake habitats and vary in species composition and abundance on seasonal and annual timescales. These factors, along with mixing at the mud-water interface, alter the information recorded in the sedimentary archive and the resulting interpretation of environmental variables in the past. Nar Gölü (central Turkey), a crater lake with annually laminated sediments, is used to investigate the link between modern and palaeo diatom assemblages. Information about the modern diatom population has been gathered through sampling of different lake habitats (1999–2009) and installing seston sediment traps in the water column (2002–2007). The palaeo diatom assemblage has been analysed through annual-resolution percentage counts and biovolume calculations on core sediments (1927–2006), along with identification of sub-annual assemblage changes through microscopic analysis of core thin sections. Comparisons of data collected using these different methods has shown a good correspondence between sediment trap and core results, but revealed that blooms of two diatom species (Nitzschia paleacea and Synedra acus), in each case occurring on average 1 year in five, can have major impacts on percentage calculations and may result in distorted and misleading palaeoenvironmental interpretations. These two species distort the average signal and may bloom for short periods of time as a result of short duration changes in lake water chemistry, such as following fresh-water input from snow melt. Blurring of sedimentary records through mixing at the sediment–water interface can mask the full magnitude of environmental variation and low sampling resolution may fail to capture high amplitude events of short duration.     

Cleaning of lake sediment samples for diatom oxygen isotope analysis

Detrital grain contamination in a diatom sample can considerably influence the δ¹⁸O_diatom signal. In order to obtain a meaningful signal, pure samples must be used. This can be achieved via a series of cleaning stages including organic and carbonate material removal, sieving, differential settling and heavy liquid separation. The method described here works best for sediments with >20% diatom content. Based on testing various clean-up methods, we propose a sequence of four clean-up stages to produce pure diatom samples from a range of lake sediments types starting with a few grams of sediment. The diatom content and the oxygen isotope composition of the samples at each stage were measured in order to assess the effect of differential amounts of contamination. Results show that a four stage clean-up is necessary to produce clean diatom samples and that contamination by silt and clay causes lower δ¹⁸O values.     

A minimal lag time and continuous sedimentation in alpine lakes near Mount Rainier, Cascade Range, Washington, USA

Alpine lakes near Mount Rainier are dammed by late-glacial moraines. Multiple cores show that sedimentation started less than 350 years after deglaciation. Numerous tephra layers provide excellent marker horizons that allow comparison of the stratigraphy without the errors associated with radiocarbon dating. Cores from different locations within a lake display the same stratigraphy and basal sediments. Core location therefore is not crucial to obtaining the oldest sediments. Continuous sedimentation in all cores is interpreted as evidence for stable lake levels throughout the Holocene. The cores are valuable tools for reconstruction and dating of glacier advances and volcanic activity.     

Combining ordination techniques and geostatistics to determine the patterns of diatom distributions at Lake Lama, Central Siberia

The patterns of spatial variation of diatom assemblages from surface sediments in Lake Lama were quantified using a combined approach of ordination and geostatistics. The aims were (i) to estimate the amount of variation between diatom assemblages within the lake, (ii) to model the spatial variability of the diatom assemblages and their diversity, and (iii) to map the diatom distributions in the lake. A correspondence analysis (CA) separated the diatom assemblages into a planktonic and a periphytic group. Rheophilic taxa were found within the periphytic group. Variogram analysis showed that only the sample scores of the first CA axis and the Shannon diversity index were spatially structured. The range of spatial correlation was estimated to be 55 km for both variables. The diversity and, to a lesser extent, the sample scores had considerable small-scale variability of about 20 and 3%, respectively. Estimates of the first component of the CA and the Shannon index were derived using block-kriging. The maps of the estimates provided a basis for partitioning Lake Lama according to the spatial structures into an eastern and a western basin, a north–south connection between the basins, and a north–south directed tip at the far eastern end. It was shown that variation in diatom assemblages is mainly spatially structured at the catchment scale and that there is a considerable amount of variation at smaller scales. According to the modeled spatial distribution, the assemblages are most likely affected by the lake size, morphology, and the water and nutrient input introduced by rivers. This has to be taken into account when paleolimnological interpretations are drawn from records of complex lake systems like Lake Lama.