南麂列岛附近海域的浮游植物和赤潮生物

１９９０年４月和１０月在南麂列岛附近海域进行了两个航次的浮游植物采样调查，共检出浮游植物９７种，隶属于３６属。春季同样浮游植物的平均丰度为２９．７×１０４个／ｍ３，水样浮游植物平均丰度达５２７６０×１０４个／ｍ３。优势种有三鳍原角藻Ｐｒｏｒｏｃｅｎｔｒｕｍｔｒｉｅｓｔｉｎｉｕｍ、夜光藻Ｎｏｃｔｉｌｕｃａｓｃｉｎｔｉｌｌａｎｓ和威氏圆筛藻Ｃｏｓｃｉｎｏｄｉｓｃｕｓｗａｉｌｅｓｉｉ。秋季浮游植物同样的平均丰度为３２．５×１０４个／ｍ３；水样浮游植物平均丰度为９２８×１０４个／ｍ３。优势类群有角刺藻Ｃｈａｅｔｏｃｅｒｏｓｓｐｐ、圆筛藻Ｃｏｓｃｉｎｏｄｉｓｃｕｓｓｐｐ、角藻Ｃｅｒａｔｉｕｍｓｐｐ、海毛藻Ｔｈａｌａｓｓｉｏｔｈｒｉｘｓｐｐ、根管藻Ｒｈｉｚｏｓｏｌｅｎｉａｓｐｐ、骨条藻Ｓｋｅｌｅｔｏｎｅｍａｃｏｓｔａｔｕｍ等。该海区春秋两季均存在多种赤潮生物。     

浙江宁波协和石油化工厂附近水域浮游植物的研究

对１９９３年８月（夏季）和１２月（冬季）在协和石化厂区附近水域获得的浮游植物样品作了鉴定和分析。结果表明,调查区共有浮游植物４门２９属６０种,可划分为三个生态类群：广布性类群、暖水性类群和温带性类群。浮游植物细胞丰度达６６×１０３～２１０７×１０３个／ｍ３。具有明显的季节变化。冬季平均细胞丰度（７４６．６×１０３±６３０．８×１０３个／ｍ３）大于夏季（２０１．８×１０３±１４６．８×１０３个／ｍ３）。主要优势种为中肋骨条藻,琼氏圆筛藻和有棘圆筛藻。浮游植物丰度的昼夜变化有随着日照增强而增高的趋势,最高细胞丰度（１０２２１×１０３个／ｍ３）出现在１２时。影响浮游植物丰度分布的主要环境因子是径流、水温和日照等。     

福建西洋岛海域浮游植物的分布

鉴定分析了于１９９０年５、８、１１月和１９９２年２月采自福建西洋岛海域的７２份样品。记录浮游植物１２７种，其中蓝藻１种、硅藻１０５种、甲藻２０种、金藻１种。浮游植物月平均细胞总量为５１．４２×１０＾４个／ｍ＾３。其年高峰出现于夏季８月，达１３７．０５×１０＾４个／ｍ＾３，低谷出现在冬季２月，仅有６．９６×１０＾４个／ｍ＾３。     

三沙湾浮游植物的分布

本文分析，鉴定了于１９９０年５，８，１１月和１９９１年２月，采自福建三沙湾的６４份样品。记录浮游植物１１５种，其中蓝藻１种，硅藻９７种，甲藻１７种。浮游植物细胞总量的年平均值为１７．５０×１＾４个／ｍ＾３，其年高峰出现于春季５月。达４３．８４×１０＾４个／ｍ＾３，低谷出现于冬季２月，仅１．１６×１０＾４个／ｍ＾３。     

象山港中、西部秋季浮游植物粒径分级、叶绿素a和初级生产力

１９９４年１０月结合水色遥感光谱实验,对象山港中、西部浮游植物细胞丰度、优势种类、叶绿素ａ浓度和初级生产力的分布做了现场观测。表层水中共鉴定出浮游植物２４种,隶属３门１７属,细胞丰度为１．０５×１０４±１．１４×１０４个／ｄｍ３;叶绿素ａ浓度为１．０７±０．５９μｇ／ｄｍ３,潜在初级生产力为３．８６±３．６０ｍｇＣ／（ｍ３·ｈ）。港区西部的浮游植物细胞丰度、叶绿素ａ浓度和初级生产力均高于港区中部;表层叶绿素ａ浓度高于中层和底层。浮游植物细胞粒径分级结果表明,微型和微微型浮游植物细胞（＜２０μｍ）对总叶绿素ａ的贡献占８６％,对初级生产力的贡献占８９％;小型浮游植物细胞（＞２０μｍ）对总叶绿素ａ的贡献占１４％,对初级生产力的贡献占１１％。     

大亚湾海域锥状斯氏藻赤潮及其与孢囊的关系

用沉积物捕捉器取样，研究了大亚湾海域２０００年６～９月孢囊（Ｃｙｓｔ）种群动态，同时 分析了浮游植物种群动态。研究期间（８～９月）该海域发生了一起大规模的锥状斯氏藻赤潮。 赤潮前一个月，锥状斯氏藻孢囊的平均日形成率为２．０５× １０５ｃｙｓｔｓ／（ｍ２·ｄ），丰度为５０．２２％， 该时期以有性生殖产生的静止期孢囊为主；在赤潮盛期的８，９月初，孢囊的平均日形成率达到 ２．０１ ×１０６ｃｙｓｔｓ／（ｍ２·ｄ），丰度为９６．９７％，此时期的孢囊多为营养细胞所形成的暂时性孢囊； 赤潮结束后，由于环境条件的恶化，暂时性孢囊的形成达到高峰，平均日形成率为１．２４×ｌ０７ ｃｙｓｔｓ／（ ｍ２·ｄ），丰度为９９.５３％。结果表明大亚湾丰富的孢囊是潜在的种源（Ｓｅｅｄ　ｂｅｄ），２０００年 的夏季、秋李持续高温（＞２５℃），台风减少及风力减弱等有利锥状斯氏藻的环境条件诱发孢 囊萌发，开始了该种类的赤潮。     

杭州湾锋区浮游植物现存量和初级生产力

１９８７年１２月和１９８８年７月，对杭州湾锋区附近海域的浮游植物细胞丰度、优势种类、叶绿素ａ浓度和初级生产力进行了现场测定。结果表明，夏季海域浮游植物细胞丰度（１９．９５×１０６个／ｍ３）高于冬季（５．６０×１０６个／ｍ３）。主要为广温低盐河口性类群和近岸广温广盐性类群占优势。夏季表层平均叶绿素ａ浓度为２．７４ｍｇ／ｍ３，冬季为１．２７ｍｇ／ｍ３。其平面分布特征表现为，表层浓度高于中层和底层，小潮汛的浓度高于大潮汛，落潮时的浓度高于涨潮时，跟踪测站的浓度高于Ｅ断面。夏季平均初级生产力为１５０．２７ｍｇＣ／（ｍ２·ｄ）。杭州湾为高悬浮物浓度的海域，光强成为初级生产力的主要限制因素之一。     

南海大鹏湾夜光藻种群在其生态群落中的地位和功能

分析了１９９１年上半年南海大鹏湾盐田水域夜光菏和浮游动、植物之间的种群动态关系，结果表明，在春季高峰期３月至５月初，调查海域共出现１２个夜光藻和浮游动、植物高峰。其中，以发生４次赤潮的夜光藻种群占主导地位，其次是枝角类的鸟啄尖头蚤，其最高数量高达９６２３８ｉｎｄ／ｍ＾３。相比之下，在夜光藻和枝角类岛啄尖头捕食压力的影响下，浮游植物的春季高峰并不显著。调查结果表明夜光藻的过度繁殖，不但对产浮游性卵的     

湛江港浮游植物与赤潮植物的初步研究

于1987年8月在湛江港设13个点采样。鉴定表明，共有浮游植物37属94种和变种；优势种类有中肋骨条藻、日本星杆藻、佛氏海毛藻等。湛江港浮游植物密度较高，港内外平均达1．3×104cell／L和7．3×105cell／L。经单项营养指数和营养状态结合指数分析表明，除两个点无机氮浓度略超标外，所有站位均未达到富营养化水平。调查期间，共发现赤潮生物31种，其中主要有中肋骨条藻、日本星杯藻、佛氏海毛藻、尖刺菱形藻和夜光藻等，中肋骨条漠和日本星杆藻曾形成水华。     

1998年春季珠江口海域大规模赤潮原因生物的形态分类和生物学特征I

对１９９８年４月１５日采集于广东珠江口万山群岛桂山岛网箱养殖区的赤潮活体水样 和福尔马林固定样品进行优势种的形态分类和生物学特征的研究。结果发现,三宅裸甲藻 （Ｇｙｍｎｏｄｉｎｉｕｍ ｍｉｋｉｍｏｔｏｉ）是此次赤潮的最优势种,最高密度达到 ７．６ × １０６ｃｅｌｌｓ／Ｌ。另两种优 势种为斯氏多沟藻（Ｐｏｌｙｋｒｉｋｏｓ　ｓｃｈｗａｒｔｚｉｉ）和螺旋螺沟藻（Ｇｙｒｏｄｉｎｉｕｍ ｓｐｉｒａｌｅ）,其最高密度分 别达到 １．１× １０５ｃｅｌｌｓ／Ｌ和 １０４－１０５ｃｅｌｌｓ／Ｌ。此次赤潮的原因生物都属于裸甲藻类,死后或用 福尔马林固定后细胞要么立即裂解,要么变形缩小。有些种类如螺旋螺沟藻在固定样品中找 不到其踪迹,因而无法精确计数。本文描述的３种赤潮藻在细胞、色素体、核、上锥沟和链状群 体等形态构造及外观上与国外学者所描述的几乎完全一致,只是细胞比日本海域产的稍小。 这可能与珠江口海域纬度较日本低有关。电镜照片显示三宅裸甲藻有一明显的上锥沟,斯氏 多沟藻则营养细胞和孢囊同时存在。     

Microbial Biomass and Organic Nutrients in the Deep-Sea Sediments of the Central Indian Ocean Basin

In order to assess the impact of deep-sea mining on the in situ benthic life, we measured the microbial standing stock and concentration of organic nutrients in the deep-sea sediments of the Central Indian Ocean Basin in the Indian pioneer area. Sediments were collected using box core and grab samples during September 1996. The total bacterial numbers ranged from 10 10 -10 11 cells per g -1 dry weight sediment. There was a marginal decrease in the number of bacteria from surface to 30 cm depth, though the subsurface section registered a higher number than did the surface. The highest numbers were encountered at depths of 4-8 cm. The retrievable number of bacteria were two orders less in comparison with the direct total counts of bacteria. An almost homogeneous distribution of bacteria, total organic carbon, living biomass, and lipids throughout the depth of cores indicates active microbial and benthic processes in the deep sea sediments. On the other hand, a uniform distribution of total counts of bacteria, carbohydrates, and total organic carbon in all the cores indicates their stable nature and suggests that they can serve as useful parameters for long-term monitoring of the area after the benthic disturbance. Further studies on temporal variability in this region would not only verify the observed norms of distribution of these variables but would also help to understand restabilization processes after the simulated benthic disturbance.     

Assessing the Validity of Seismo-Acoustic Predictor Equations for Obtaining Seabed and Sub-Surface Sediment Physical Properties

The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths.     

Geoacoustic Inversion via Genetic Algorithm and Its Application to Manganese Sediment Identification

An acoustic inversion method using a wide-band signal and two near field receivers is proposed and applied to multiple layered seabed models including a manganese sediment. The inversion problem can be formulated into a probabilistic model comprised of signals, a forward model, and additive noise. The forward model simulates wide-band signals, such as chirp signals, and is chosen to be the source-waveletconvolution plane wave modeling method. The wavelet matching technique, using weighted least-squares fitting, estimates the sediment sound-speed and thickness on which determination of the possible numerical ranges for a priori uniform distribution is based. The genetic algorithm is applied to a global optimization problem to find a maximum a posteriori solution for determined a priori search space. Here the object function is defined by an L 2 norm of the difference between measured and modeled signals. Not only the marginal pdf but also its statistics are calculated by numerical evaluation of integrals using the samples selected during importance sampling process of the genetic algorithm.     

海上大直径钢管桩打桩过程中桩周土强度弱化研究

海上大直径钢管桩打桩过程中,桩周土体受到强烈扰动而发生强度弱化,掌握桩周土体强度弱化规律对于准确预测打桩过程、保证工程安全具有重要意义。为研究土体强度弱化规律,开展了环剪试验模拟打桩对桩周土体的扰动,测试土体强度随剪切速率的变化规律,建立了描述土体强度弱化规律的拟合公式,引入到打桩分析软件中。研究结果表明：土体的强度折减程度不仅与土体本身的性质有关还受到土体的埋深和剪切速率的影响,埋深越深土体强度折减程度越低,剪切速率越高土体强度折减越高,在打桩分析中可采用这里推荐的线性折减方法来模拟不同深度处土体强度的折减规律。     

Interannual Sea Level Variations and Annual Tides in the Northwestern Pacific

Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone.     

Deep-Sea Sediment Compression Curves: Some Controlling Factors, Spurious Overconsolidation, Predictions, and Geophysical Reproduction

Oedometer tests have been carried out on 70 undisturbed surficial clays (at approximately 250 mm below the mudline), mostly collected by free-fall corers from sites widely scattered throughout the deep-sea North Atlantic. Acoustic measurements were also made, initially on contiguous samples and ultimately on the same sample using a geophysically instrumented oedometer which also collected electrical resistivity data. Apart from those quiescent areas below the carbonate compensation depth, such as north of the West Indies where very fine clays exist, most of the samples are silty clays whose geotechnical-geophysical properties are dependent on the type of clay minerals present (and their ability to take in moisture), the sand-size fraction, and the quantity of carbonate present. Thus the pure clays have high compressibilities which decrease on the addition of coarse particles, while the converse is true for the acoustic parameters, these increasing with the sand fraction. Using the notion of the intrinsic compression line for all samples, and comparison to it of the measured compression curves, it is clear that, contrary to some previously held ideas, most deep-sea clays are normally consolidated; the addition of carbonate has the effect of creating an open, stronger sediment skeleton. Interestingly, where information is available, the variation with depth of a sample's acoustic velocity follows the void ratio pressure relationship of the compression curve. This allows the construction of an in-situ sediment compression curve using the in-situ geophysical observations.     

Flow Cytometric Analysis of Coastal Lagoon Bacterioplankton and Picophytoplankton: Fixation and Storage Effects

The effect of different fixation and storage protocols on the flow cytometric (FCM) simultaneous analysis of bacterioplankton and phytoplankton in coastal seawater samples (Mediterranean coastal lagoons) was investigated. FCM measurements (cell number, fluorescence and scatter characteristics) were obtained through DAPI staining. Three fixatives [glutaraldehyde (GA), formaldehyde (FA) and paraformaldehyde (PFA)] and two storage (3 months duration) methods (5 °C and −196 °C) were tested. Two dominant populations were detected in studied samples: bacteria and eukaryotic picophytoplankton. Adding fixatives (2% final concentration) appears necessary to obtain FCM exhaustive counts of all the bacteria and phytoplanktonic cells. This was related to the permeation effect of fixatives which allowed a better DAPI staining of the cells. Maximum fluorescence, i.e. optimal staining of the cells was obtained with FA or PFA, and significant lower fluorescences with GA. Fixed samples stored at 5 °C induced rapid cell loss. Only storage in liquid nitrogen of samples fixed with FA or PFA, allows mid-term (≥4 months) preservation of bacteria or picophytoplankton cell numbers, and limited evolution of DAPI-induced fluorescence and scatter characteristics.     

Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans

A set of 27 marine planktonic bacteria isolated from the polar regions was characterized by 16S rDNA sequencing and physiological and biochemical testing. More than half of these bacteria were positive for caseinase, gelatinase and 13-glucosidase, and could utilize glucose, maltose or malic acid as carbon source for cell growth. Twelve isolates expressed nitrate reduction activities. Except for one antarctic isolate BSwlO175 belonging to Actinobacteria phylum, these isolates were classified as γ-Proteobacteria, suggesting that γ-Proteobacteria dominated in cultivable marine bacterioplankton at both poles. Genus Pseudoalteromonas was the predominant group in the Chukchi Sea and the Bering Sea, and genus ShewaneUa dominated in cultivable bacterioplankton in the Prydz Bay. With sequence similarities above 97%, genus Psychrobacter was found at both poles. These 27 isolates were psychrotolerant, and significant 16S rDNA sequence similarities were found not only between arctic and antarctic marine bacteria （ 〉 99% ）, but also between polar marine bacteria and bacteria from other aquatic environments （ ≥ 98.8% ）, including temperate ocean, deep sea, pond and lake, suggesting that in the polar oceans less temperature-sensitive bacteria may be cosmopolitan and have a bipolar, even global, distribution at the species level.