Towards a more ecologically relevant assessment of the impact of heavy metals on the photosynthesis of the seagrass, Zostera capricorni

This in situ study used photosynthetic activity (measured as chlorophyll a fluorescence) and photosynthetic pigment concentrations to assess the effect of copper, cadmium, lead and zinc on the seagrass Zostera capricorni. Custom-made portable in situ exposure (PIE) chambers were developed so seagrasses could be dosed within the meadow. Z. capricorni was exposed to 0.1 and 1 mg l⁻¹ of metal solutions for 10 h. During this time and for the subsequent four-day recovery period, the effective quantum yield of photosystem II (PS II) (ΔF/Fm^′) was measured. While the results were variable, copper and zinc exposed samples had a depressed ΔF/Fm^′ during the exposure period. Samples exposed to zinc recovered to pre-exposure levels but those exposed to copper did not. Cadmium and lead did not impact on the chlorophyll a fluorescence and the chlorophyll pigment data supported these findings. This study presents an innovative new application of chlorophyll a fluorescence stress assessment.     

Characteristics of ambient stress values for micro－earthquake sequences in Western Yunnan Earthquake Prediction Experimental Field

Ｃｈａｒａｃｔｅｒｉｓｔｉｃｓｏｆａｍｂｉｅｎｔｓｔｒｅｓｓｖａｌｕｅｓｆｏｒｍｉｃｒｏ－ｅａｒｔｈｑｕａｋｅｓｅｑｕｅｎｃｅｓｉｎＷｅｓｔｅｒｎＹｕｎｎａｎ　Ｅａｒｔｈｑｕａｋｅ　Ｐｒｅｄｉｃｔｉｏｎ　Ｅｘｐｅｒｉｍｅｎｔａｌ　ＦｉｅｌｄＪｉａ－Ｚ...     

大型溞(Daphnia magna)线粒体基因组的测定与序列分析

利用Long-PCR及普通PCR技术,结合引物步移法测序,经拼接、组装后获得中国淮河大型溞(Daphnia magna)线粒体基因组全序列,并对其基因组成、结构特点等进行初步分析.大型溞线粒体基因组全长为14948 bp,A、T、G、C各碱基含量分别为32.37%、34.73%、15.58%、17.31%,表现出明显的AT偏倚.13个蛋白质编码基因、22个t RNA基因、2个rRNA基因和1个D-loop区(控制区)的排列方式与典型的节肢动物线粒体基因组的排列方式略有不同.除COI基因以CTG、ATP8基因以GTG、ND3基因以ATC以及ND6基因以ATT为起始密码子以外,其余9个蛋白质编码基因均以ATG作为起始密码子;9个蛋白质编码基因具有典型的完全终止子TAG或TAA,COI、COII、ND4和ND5等基因采用不完全终止密码子T.基因组包含9个基因间隔区,共81 bp,长度1~62 bp;13个基因重叠区,共77 bp,重叠碱基数在1~27 bp之间,最大重叠在16 S rRNA和tRNA~(Val)基因之间.在预测的22个tRNA基因的二级结构中,发现只有tRNA~(Ser1)基因未能形成完整的二级结构,其他21个基因均可形成典型的三叶草结构.16 S rRNA和12 S rRNA基因长度分别为1373 bp和752 bp,D-loop区全长289 bp.本研究结果为探讨大型溞在溞属中的系统学地位及其与其他种间的系统发生关系等问题提供了数据资源.     

Study on the property of apparent resistivity changes of rock samples by in situ shear and friction test

In the strip limestone mine in Guiding county, Guizhou Province the shear and frictionin situ tests of rock body were made for the three typical inclined weak bands C ₃ ¹ /C ₃ ¹ , C ₃ ¹ /C ₂ ² and C ₂ ² /C ₂ ¹ . The tests were made according to the second scheme of cuneate sample of the standards on rock mechanics test of Water Conservancy and Electricity Ministry. The changes of the resistivity in the weak band and the acoustic speed across the weak band were measured in the same time. The apparent resistivity data, obtained for 8 samples on 27 measure lines in 38 cycle tests, show that the apparent resistivity changes have rather obvious characters as follows: 1. At shear and friction stage, the change of the apparent resistivity accelerates after the yield point, and reaches the maximum of change rate and change amplitude near fracture point (except the lines with resistivity invariant); 2. On the same sample, the resistivity changes are different on the various lines and related to the location settled the lines, there are some “sensitive” location; 3. At the stage of preloading normal stress before shearing, the resistivity decreases on most lines, but on a few lines the resistivity does not changes; 4. After unloading shear stress, the resistivity could not recover completely and the hysteresis of resistiviity takes place on a few lines. The Chinese version of this paper appeared in the Chinese edition ofActa Seismologica Sinica,15, 217–223, 1993. Support for this research was received from Guiding strip limestone mine, Guizhou Organic Chemistry Factory. This research is supported by the Chinese Joint Seismological Science Foundation.     

Continuing observation of disturbed reproductive cycle and ovarian spermatogenesis in the giant abalone, Haliotis madaka from an organotin-contaminated site of Japan

Histological examination of gonads as well as chemical analysis of organotin compounds in tissues of the giant abalone, Haliotis madaka, was conducted to evaluate continuing endocrine disruption in abalone populations in Japan. Abalone specimens were collected from two different areas, Tsushima as a reference site and Jogashima as a site representative of declining abalone populations where serious organotin contamination had been observed, each month from January 1998 to March 1999. Scores were given to the development stages of reproductive cells in the ovary and testis. The degree of sexual maturation was evaluated by calculating the mean value of a histogram of these scores for the reproductive cells of each abalone. The temporal variations in degree of sexual maturation showed that female and male abalone from Tsushima matured synchronously, while those from Jogashima did not, which were similar to results of the previous study during September 1995–November 1996. Approximately 19% of the female abalone from Jogashima were masculinized with an ovo-testis, which was also similar to the result of the previous study. The masculinization of female abalone is reported to be induced by tributyltin (TBT) and triphenyltin (TPhT) from antifouling paints. Concentrations of the sum of butyltins (TBT, dibutyltin (DBT) and monobutyltin (MBT): ΣBTs) and the sum of phenyltins (TPhT, diphenyltin (DPhT) and monophenyltin (MPhT): ΣPhTs) in the muscle of abalone from Jogashima (n = 73) of 7.8 ± 9.0 ng/g wet wt. and 4.5 ± 6.8 ng/g wet wt., respectively, were significantly higher than those from Tsushima (n = 87) of 4.7 ± 4.9 ng/g wet wt. and 0.8 ± 1.7 ng/g wet wt., respectively (p < 0.05 for ΣBTs; p < 0.001 for ΣPhTs), although concentrations of TBT and TPhT in the muscle of abalone from Jogashima (n = 73) of 2.2 ± 2.5 ng/g wet wt. and 5.8 ± 5.1 ng/g wet wt., respectively, were insignificantly and significantly higher than those from Tsushima (n = 87) of 0.4 ± 0.6 ng/g wet wt. and 0.5 ± 0.9 ng/g wet wt., respectively, (p > 0.05 for TBT; p < 0.001 for TPhT). Thus, endocrine disruption as well as contamination by organotins in the giant abalone from Jogashima is still persisting.     

新源、和静交界ML6.8级地震序列应力降和震源机制研究

Based on the digital waveform data recorded by Xinjiang Digital Seismic Network for the Xinyuan-Hejing M_L6.8 earthquake sequences of June 30,2012,this paper analyzes the stress drops of earthquake sequences and the correlation coefficients of focal mechanisms significant for strong aftershocks.Firstly,the source parameters of the Xinyuan-Hejing M_L6.8 earthquake sequences are obtained by applying the spectrum analysis and the Brunes source model.Then,the correlation coefficients of spectral amplitudes are calculated using the low-frequency spectral amplitude recorded by the same station for the different events.Finally,based on the results of the correlation coefficients of spectral amplitudes,the events with similar focal mechanisms are grouped using the clustering method.The results show that:(1)The stress drop values show a steady trend in the aftershock sequence calm period and the stress drop values show a rise-fall in strong aftershocks.(2)The moving average correlation coefficient of amplitude spectrum begins to spread after the main shock.It shows that the correlation decreases between the main shock and the aftershocks in mechanisms.(3)The results of focal mechanism groups show that the earthquake sequences are mainly strike slips.The stress distribution of the main pressure axis is nearly NS,which is the same as the structural stress field.(4)The magnitude and mechanism show that there is an agreement before the strong aftershock,which shows that the regional stress field is enhanced.     

A study on the focal parameters of Shidian M =5.9 earthquake sequences in 2001

On the basis of about 300 earthquake wave forms observed in the Shidian M _S=5.9 sequences on April 12, 2001 recorded in Kunming Digital Seismic Network, the spectra of shear wave have been used to estimate the focal parameters of these earthquake sequences. The results show that within the magnitude range of 1.5–5.3, the seismic moments are 10¹⁰–10¹⁶ N·m, the corner frequencies are 0.2–0.8 Hz, radii of the focal rupture are 200–2 500 m and the stress drops are 0.1×10⁵–20×10⁵Pa. Through the statistical analyses of variation of corner frequency f _c and stress drop Δσ with time, it is discovered that the average corner frequency of the foreshock sequences is obviously lower than that of the aftershock sequences. Contrarily, the average stress drops Δσ of the foreshock sequences are clearly higher than that of the aftershocks. It is considered that these variation characteristics of average corner frequency and stress drops before and after the main shock have index significance to the precursory information before a strong earthquake. The higher stress drops for the foreshock sequences show that the higher shear stresses have been stored in the area of main shock. After the main shock, most of the stresses have been released, so the aftershock sequences show a rupture process of lower stresses. Foundation item: Scientific and Technological Key Project of Yunnan Province (2001NG46)     

利用real-time RT-PCR研究大型蚤对铜绿微囊藻毒素合成基因转录水平影响

近年来关于浮游动物与微囊藻相互作用的研究逐渐被关注.其中有的研究认为浮游动物能够诱导产毒细胞毒素含量的变化.微囊藻毒素是由微囊藻毒素合成基因编码翻译的,目前关于浮游动物对微囊藻毒素合成基因相对表达的影响并无报道,本文首次通过实时定量逆转录PCR方法研究铜绿微囊藻PCC7806产毒相关基因mcyB和mcyD在大型蚤胁迫下...     

2016年新疆呼图壁6.2级地震前b值异常特征研究

利用2009年以来中国地震台网中心地震编目资料,以2016年新疆呼图壁6.2级地震作为研究对象,采用最大似然法进行b值空间扫描计算,获取震前震中及其邻区地震b值的空间图像。研究结果显示,2016年呼图壁6.2级地震发生在震前显著低b值区域,该异常特征可能反映了地震孕育的应力积累过程,印证了b值的物理意义。     

Study of the Interaction Mechanism in the Biosorption of Copper(II) Ions onto Posidonia oceanica and Peat

A systematic approach was used to characterize the biosorption of copper(II) onto two biosorbents, Posidonia oceanica and peat, focusing on the interaction mechanisms, the copper(II) sorption–desorption process and the thermal behavior of the biosorbents. Sorption isotherms at pH 4–6 were obtained and the experimental data were fitted to the Langmuir model with a maximum uptake (q_max) at pH 6 of 85.78 and 49.69 mg g^?1, for P. oceanica and peat, respectively. A sequential desorption (SD) with water, Ca(NO₃)₂, and EDTA was applied to copper‐saturated biosorbents. Around 65–70% copper(II) were desorbed with EDTA, indicating that this heavy metal was strongly bound. The reversibility of copper(II) sorption was obtained by desorption with HCl and SD. Fourier transform IR spectroscopy (FTIR) analysis detected the presence of peaks associated with OH groups in aromatic and aliphatic structures, CH, CH₂, and CH₃ in aliphatic structures, COO^? and COOH groups and unsaturated aromatic structures on the surface of both biosorbents, as well as peaks corresponding to Si? O groups on the surface of peat. The results of SEM‐EDX and FTIR analysis of copper‐saturated samples demonstrated that ion exchange was one of the mechanisms involved in copper(II) retention. Thermal analysis of biosorbent samples showed that copper(II) sorption–desorption processes affected the thermal stability of the biosorbents.     

PBDEs in the blubber of marine mammals from coastal areas of São Paulo, Brazil, southwestern Atlantic

Limited information is available in the literature on the levels of brominated flame retardants in the southern hemisphere. This study presents concentrations of polybrominated diphenyl ethers (PBDEs) in the blubber of small cetaceans from the coast of São Paulo (Brazil), southwestern Atlantic. PBDE levels were highest in Stenellafrontalis (770 ng g⁻¹ lipid) followed by Stenobredanensis (475 ng g⁻¹ lipid), Sotaliaguianensis (65.6 ng g⁻¹ lipid), Tursiopstruncatus (64.2 ng g⁻¹ lipid) and Pontoporiablainvillei (60.3 ng g⁻¹ lipid). In general, continental shelf individuals exhibited higher contamination than inshore animals. This might be related to larger prey items consumed by continental shelf dolphins. The pattern of contamination indicates that Penta-BDE commercial mixtures are a major source of PBDEs to top predators in the southwestern Atlantic. Congeners found in Octa-BDE formulations were not detected in the investigated animals.     

低浓度硝态氮促进微囊藻累积多聚磷酸盐

2016年以来太湖总磷浓度高位波动而总氮浓度持续下降,藻细胞内源性磷释放是湖泊水体总磷的重要来源,而多聚磷酸盐作为藻细胞内磷的储存库,其含量变化会显著影响藻细胞内源性磷的释放量.针对上述现象,开展了不同硝态氮浓度影响野外水华蓝藻及实验室纯培养的铜绿微囊藻(Microcystis aeruginosa)利用磷特别是合成多...     

Seismic intensity investigation of the 2001 M =6.0 Yajiang-Kangding earthquake and discussion on its background of seismogenic structures

An M=6.0 earthquake occurred on February 23, 2001 in the western Sichuan Province, China. The macro seismic epicenter situated in the high mountain-narrow valley region between Yajiang and Kangding counties. According to field investigation in the region, the intensity of epicentral area reached VIII and the areas with intensity VIII, VII and VI are 180 km², 1 472 km² and 3 998 km², respectively. The isoseismals are generally in elliptic shape with major axis trending near N-S direction. The earthquake destroyed many buildings and produced some phenomena of ground failure and mountainous disasters in the area with intensity VIII. This event may be resulted from long-term activities of the Litang fault and Yunongxi fault, two main faults in the western Sichuan. The movements between the main faults made the crust stress adjusted and concentrated, and finally the earthquake on a secondary fault in the block released a quite large energy.     

一株淡水红藻的形态及分子系统发育研究

以采自山西晋祠泉的一株淡水红藻JC1712001为实验材料,对其进行分子系统发育分析和形态特征鉴定,同时利用分子数据对其进行生物地理学研究.结果显示,该株淡水红藻JC1712001与美国、西班牙地区报道的马赫拉熊野藻（Kumanoa mahlacensis Kumano）遗传距离最近,基于rbcL、COI和UPA序列的遗传距离均为0.基于3个序列,利用贝叶斯法、最大似然法和邻接法3种方法构建的系统发育树具有高度一致的系统发育关系,JC1712001均与西班牙及美国地区已报道的马赫拉熊野藻聚为一支,支持率都在97%以上,支持其为马赫拉熊野藻,同时也表明3个基因的高度保守性.形态特征的观察也支持这个结果.马赫拉熊野藻在欧洲、亚洲、北美洲均有分布,RASP软件构建的地理起源图谱显示,欧洲类群地理起源更为古老.马赫拉熊野藻为稀有种,本文的研究结果丰富了我国淡水红藻的分布,为其系统发育和地理起源研究提供了依据.     

Scattering and anelastic attenuation of seismic energy in northern Greece

The relative contribution of scattering (Q _s ^–1 ) and intrinsic (Q _i ^–1 ) attenuation to the totalS-wave attenuation for the frequencies of 1.5, 3.0, 6.0 and 12.0 Hz has been studied by applying the radiative energy transfer theory, Data of local earthquakes which occurred in northern Greece and were recorded by the permanent telementered network of the Geophysical Laboratory of the University of Thessaloniki have been used. The results show that in this area the scattering attenuation is dominant over all frequencies while intrinsic attenuation is significantly lower. The estimatedQ _s ^–1 andQ _i ^–1 values have frequency dependences off ^–0.72 andf ^–0.45, respectively. The frequency dependence ofQ _s ^–1 is the same as that of the codaQ _c ^–1 , obtained by applying the single scattering model, which probably implies that the frequency dependence of the coda wave attenuation is attributed to the frequency dependence of the scattering attenuation.Q _c ^–1 values are very close to scattering attenuation for short lapse times, (10–20 sec), and intermediate between scattering and intrinsic attenuation for the longer lapse times, (50–100 sec). This difference is explained as the result of the depth-dependent attenuation properties and the multiple scattering effects.     

Application of apparent stress in earthquake prediction

On the basis of the assumption that ω2 model accords with source displacement spectra of moderate-small shocks, we calculate the apparent stress values of 1 020 moderate-small shocks recorded by the Digital Seismic Network of Yunnan Province by using the low-frequency flat level and corner frequencies. The results show that the apparent stress is of good significance in earthquake prediction. The moderate-small shock with apparent stress larger than 0.9 MPa can be used as a referential index to predict moderate-strong earthquakes in Yunnan area. And its relevant predictability evaluation (R value) has a high confidence level.     

The toxicity and oxidative stress of TiO2 nanoparticles in marine abalone (Haliotis diversicolor supertexta)

The increasing use of nanotechnology highlights the need to understand and clarify the environmental impacts of nanomaterials. In this study, the acute toxicity and oxidative stress of titanium dioxide nanoparticles (nTiO₂) in mature marine abalone (Haliotis diversicolor supertexta) were assessed. No acute effect was found in any of the treatment groups with nTiO₂ concentration gradients ranging from 0.1 to 10 mg/L. However, the activity of an antioxidant enzyme superoxide dismutase (SOD) significantly increased in the group that was exposed to 1.0 mg/L nTiO₂. The content of a non-enzymatic antioxidant, glutathione (GSH), significantly decreased in the groups with an nTiO₂ concentration ⩾1.0 mg/L. The level of lipid peroxidation (LPO) was found to increase as the nTiO₂ dose increased. Furthermore, NO was produced in excess in abalone. These results demonstrated that, although nTiO₂ is not acutely toxic to abalone, it does exert oxidative stress on abalone.     

冷季不同植物人工湿地处理生活污水的工程实例分析

本实验以亚热带地区福建省永春县农村生活污水作为处理对象,选取10种湿地植物,构建具有不同植物类型的表面流人工湿地的实例工程,比较不同植物配置人工湿地的处理能力,以及湿地植物的生物量和植物体内氮、磷累积能力.研究结果表明,水龙和美人蕉人工湿地具有较高的铵态氮去除能力,去除率可分别达到76.7%和87.7%;两者同时也对总氮表现出较好的去除效果,尤其是美人蕉人工湿地在冬季节对总氮的去除率可维持在80.3%以上;2014年初冬季节实验结果表明,水龙还具有一定的总磷去除能力,去除率可达到69.0%.水龙和美人蕉具有较大的生物量,其单位面积干重可分别达到0.46和0.30 kg/m~2,又能较好地积累氮、磷元素,其体内氮含量可分别达到6.43和4.60 g/m~2,磷含量可分别达到0.50和1.01 g/m~2.综上所述,种植水龙和美人蕉的人工湿地总体处理效果较好,同时又能更多地积累氮、磷元素,且具有更大的生物量,可选为相对适宜用于处理生活污水的适合亚热带地区应用的人工湿地植物.     

Coda Q in the Kachchh Basin, Western India Using Aftershocks of the Bhuj Earthquake of January 26, 2001

Q_C-estimates of Kachchh Basin in western India have been obtained in a high frequency range from 1.5 to 24.0 Hz using the aftershock data of Bhuj earthquake of January 26, 2001 recorded within an epicentral distance of 80 km. The decay of coda waves of 30 sec window from 186 seismograms has been analysed in four lapse time windows, adopting the single backscattering model. The study shows that Q_c is a function of frequency and increases as frequency increases. The frequency dependent Q_c relations obtained for four lapse-time windows are: Q_c=82 f^1.17 (20–50 sec), Q_c=106 f^1.11 (30–60 sec), Q_c=126f^1.03 (40–70 sec) and Q_c=122f^1.02 (50–80 sec). These empirical relations represent the average attenuation properties of a zone covering the surface area of about 11,000, 20,000, 28,000 and 38,000 square km and a depth extent of about 60, 80, 95, 110 km, respectively. With increasing window length, the degree of frequency dependence, n, decreases marginally from 1.17 to 1.02, whereas Q₀ increases significantly from 82 to 122. At lower frequencies up to 6 Hz, Q_c⁻¹ of Kachchh Basin is in agreement with other regions of the world, whereas at higher frequencies from 12 to 24 Hz it is found to be low.