An evaluation of hemolymph cholinesterase activities in the tropical scallop,Euvola (Pecten) ziczac,for the rapid assessment of pesticide exposure

The use of sequential measurements of hemolymph cholinesterase activities as a non-invasive biomarker of seasonal organophosphate/carbamate exposure was investigated for the tropical scallop, Euvola (Pecten) ziczac. Overall activities of both acetylcholinesterase and butyrylcholinesterase were relatively high compared to studies with bivalve tissues. Acute in vivo experiments showed inhibition of hemolymph acetylcholinesterase activity at concentrations of the organophosphate insecticide chlorpyrifos of 0.1, 1 and 10 ng l(-1). Monthly sampling of hemolymph from scallops at two sites in Bermuda over a 15 month period showed seasonal acetylcholinesterase and butyrylcholinesterase inhibition. Direct and indirect evidence suggests that this inhibition did not relate to biochemical or physiological changes associated with gonad maturation and spawning, but rather reflected diffuse contamination of the marine environment by cholinesterase inhibitors or increased bioavailability of such inhibitors at these times. Repetitive sampling of scallop hemolymph for cholinesterase activities represents a rapid, sensitive and non-invasive method for assessing seasonal, sublethal pesticide exposure in these commercially important bivalves and suggests a wider use in marine pollution monitoring.     

Seasonal variations of biomarker responses in the marine blue mussel (Mytilus spp.)

Biomarkers, which can detect changes at the biochemical level, have been used for many years as early warning tools in environmental monitoring. In order to distinguish between natural variability and the potential effects of anthropogenic pollution, it is essential to identify background levels and establish the potential influence of abiotic (season, temperature and salinity) and biotic (gametogenesis) factors. In this study, we examined various biomarkers of stress (glutathione S-transferase (GST)), reproduction (vitellin-like proteins) and damage (lipid peroxidation (LPO) and DNA damage (DNA)) in marine mussels (Mytilus spp.) from a known pristine hybrid zone. Levels of all biomarker expression varied between seasons and appeared to be linked to the reproductive cycle. Oxidative stress in winter, with low GST expression and a higher expression of LPO and DNA damage displayed could be explained by low food availability. These data provide vital baseline information for future ecotoxicological and environmental monitoring studies.     

Distribution of contaminants in biota and sediments in the Musquash Estuary, Atlantic Canada, marine protected area site initiative and contaminant exclusion zone

The Musquash Estuary, one of the last ecologically intact estuaries in New Brunswick, has been designated an area of interest for a marine protected area (MPA) under the Oceans Act. The area has been assessed for contaminant background levels as required for establishing MPA environmental quality. American lobster (Homarus americanus), blue mussel (Mytilus edulis) and sediments were collected for assessing contaminant levels and distribution in the harbour. Levels of contaminants from the indicator species and the abiotic component have shown: (1) two extremes of high and low Cu and Ag in lobster from the area; and (2) lower metal levels in inner Musquash Harbour sediments and mussels than in those from the harbour mouth. These suggest that deposition of contaminants into the Musquash MPA site was due to transport of contaminants by coastal currents from upstream coastal industrial activities. This reverse trend with higher contaminant levels in the biotic and abiotic components in the outer harbour than in the inner harbour differs from a contaminated harbour and suggests that a contaminant exclusion zone should be considered for controlling contamination from nearby coastal and estuarine industrial sites to protect the sensitive habitats within the marine protected area.     

Local-scale cross-correlation of seismic noise from the Calico fault experiment

Most studies of seismic noise cross-correlation (NCC) have focused on regional/continental scale imaging using empirical surface-wave Green’s functions extracted from primary (0.05–0.08 Hz) and secondary (0.1–0.16 Hz) microseisms. In this work, we present the NCC results at higher frequencies (>0.5 Hz) from 6 months seismic noise recorded by a local array (~4 km aperture) deployed along the Calico fault in the Mojave Desert, California. Both fast and slow propagating waves are observed from the NCC record-sections. We compare the NCCs from sensor pairs that share a common sensor with the records of a borehole shot located very close to this common sensor. The result shows a good match of the slow surface-wave arrivals, indicating that the NCC method is able to recover unbiased surface-wave Green’s functions at local scales. The strong body-wave NCC component is caused by the P waves generated offshore California. Along a SW–NE profile across the fault, we observe apparent P-wave arrivals and their reflections, which can be explained by a low-velocity-zone (LVZ) along the Calico fault. We calculate the LVZ width to be ~ 2.3 km, and the P-wave velocity reduction within the LVZ to be ~35 %. These estimates are consistent with other evidence for a relatively wide LVZ along the Calico fault.     

Marine environmental impact due to mining activities of El Salvador copper mine,Chile

Marine environmental impact as a result of copper mine tailing dumping in the sea is studied at Chañaral (North of Chile). These dumpings, from the copper mine El Salvador, have hindered harbour activities, caused geomorphological coastal modifications and affected seriously marine coastal ecosystems and recreational activities. At the old dumping site, Chañaral Bay (1938–1974), nearly 150 million tons of fine sediments were accumulated. Tailing discharges at the new dumping site, Caleta Palito, about 8 km north of Chañaral Bay are accumulating approximately at the rate of 25 000 tons of fine sediments per day, plus unknown quantities of chemicals (Cu, As, CN^?). From January 1975 to July 1976, this site has received over 13 million tons of sediments which has caused deterioration of the marine coastal environment, reduction in light penetration and high mortalities among marine invertebrates, fishes and algae.     

南黄海海底大地电磁测深试验研究

针对南黄海盆地深层地震地质条件差,地震波受屏蔽,能量严重衰减,在海洋反射地震资料中很难识别反映中-古生界的反射地震波组,不能满足南黄海盆地前第三系油气资源前景评价的需要等问题,于2006年5月,开展了南黄海海域海底大地电磁测深试验,在盆地内五莲斜坡和胶州凹陷区布设了3个海底大地电磁测深点,取得了可靠的海底观测数据.本文详细阐述了海底大地电磁测深与常规大地电磁测深在数据处理方法上的差别,论述了海洋电磁噪声对海底大地电磁场观测的影响,提出了海底大地电磁场实测资料“水平姿态”校正、“方位”校正和“海洋电磁噪声”校正的方法.通过对试验观测资料的处理和反演,并结合地质、物性资料进行综合分析,结果表明在南黄海盆地海底以下深部还存留有古生代地层,这对于南黄海前第三系含油气前景的评价具有重要意义.试验结果也证明了海底大地电磁测深技术在研究古生代残留盆地方面的问题可以发挥相当好的作用.     

Historical and ecological analysis of coral communities in Castle Harbour (Bermuda) after more than a century of environmental perturbation

The coral reefs in Bermuda's Castle Harbour basin have been subjected to varying anthropogenic stressors for over 100 years. These include restriction of water flow through the construction of a causeway in the late 19th century and an extensive dredging and land reclamation operation during World War II. In the 1970s, disposal of bulk waste commenced at a foreshore reclamation site in Castle Harbour. Since 1996 the waste stream has included blocks of cement-stabilized municipal incinerator ash. This study provides a historical and quantitative ecological review of the Castle Habour reef ecosystem as a case study, assessing the responses of the reef to more than a century of anthropogenic disturbance. Measures of the coral community, flow rates, turbidity and sedimentary regimes suggest the present structure of the coral community largely reflects the impacts of the historic dredge and fill operations prior to the establishment of the foreshore dump site. Recent increases in the abundance of some sediment tolerant, massive reef-building coral species (Diploria strigosa and Montastraea cavernosa) suggest adaptation to chronic sediment stress.     

Biomarkers of exposure and effect in the Brazilian flounder Paralichthys orbignyanus (Teleostei: Paralichthyidae) from the Patos Lagoon estuary (Southern Brazil)

Biomarkers of exposure (liver metallothionein-like proteins content and catalase and glutathione S-transferase activities) and effect (liver lipoperoxidation and blood cell DNA damage) of contaminants were analyzed in the Brazilian flounder Paralichthys orbignyanus from the Patos Lagoon estuary (Southern Brazil). Flounders were collected for a year in two sites: "Coroa do Boi" (polluted site) and "Saco do Justino" (non-polluted site). Results indicated that micronucleated cells frequency was the best biomarker to distinguish flounders from the two sites. Taken together, data from DNA damage analyses (micronucleus test and comet assay) indicated that flounders from the non-polluted site efficiently repaired the DNA breaks, contrary to those from the polluted site, which probably had their DNA repair system inhibited or exhausted. Furthermore, data from enzyme activities (catalase and GST) and lipid peroxidation indicated that flounders from the polluted site were under oxidative stress in summer and autumn.     

Biomarkers of exposure and effect in flounder (Platichthys flesus) exposed to sediments of the Adriatic sea.

The modifications of several biomarkers were investigated in flounder (Platichthys flesus) when exposed in the laboratory to sediment samples collected from the Northern Adriatic Sea. Besides clean sand used as a control substrate, fish were exposed to sediments sampled offshore the delta of the Po River, the harbour of Trieste, and from the industrial harbour of Venice (Porto Marghera). After six days of exposure, the enzyme activities ethoxyresorufin-O-deethylase (EROD), UDP glucuronyltransferase (UDPGT), glutathione S-transferase (GST), glutathione reductase (GR) and glutathione peroxidase (GPx), were assayed in fish liver. In addition, the contents of reduced glutathione (GSH), nonprotein thiols (SH), total sugars and extractable lipids were also determined in hepatic tissue, as well as the number of micronucleated hepatocytes and biliary concentrations of fluorescent aromatic compounds (FAC). Despite some variability within treatment groups, differences among exposed organisms were evident and consistent with known contaminant levels of sampled areas. Microsomal activities (EROD, UD-PGT) and FAC levels were the most sensitive exposure indicators. Variations in the other biomarkers showed only tendencies which although not statistically significant were generally consistent with the contamination pattern.     

Concentration and distribution of 210Po in the tissues of the scallop Chlamys varia and the mussel Mytilus edulis from the coasts of Charente-Maritime (France)

Polonium-210 (210Po) has been analysed in the soft parts of two bivalves species, the scallop Chlamys varia and the common mussel Mytilus edulis, from the Bay of La Rochelle and Ré Island, on the French Atlantic coast. Between those sites, the highest 210Po concentrations have been found in whole scallop soft parts from La Rochelle, reaching 1,181 +/- 29 Bq kg(-1) dry weight (dwt), a size effect being related to the highest 210Po concentration in the smallest scallops. The results show a significant difference in concentrations for similar size individuals between species in each site (C. varia > M. edulis) and between sites for each species (Ré Island > Bay of La Rochelle). Very high 210Po concentrations have been found in the digestive gland of C varia, ranging 3,150-4,637 Bq kg(-1) dwt. Thus, the digestive gland contains up to 60% of the radionuclide. Subcellular investigations have shown that approximately 40% of the 210Po contained in the digestive gland is in the cytosolic fraction, suggesting a high bioavailability of the 210Po from this fraction to the trophic upper level. Calculations will show that approximately 4 kg of scallops flesh intake would be necessary to reach the annual incorporation limit of 1 mSv.     

Impact of digenean parasite infection on metallothionein synthesis by the cockle (Cerastoderma edule): a multivariate field monitoring

Metallothioneins (MT) are proteins that play an important role in metabolism of essential metals and detoxification of trace metals from living organisms. Their synthesis is induced by metal pollution but can also be exacerbated by other factors such as reproduction processes. In this context, we monitored MT concentrations in a cockle Cerastoderma edule (marine bivalve) population and highlighted the effect of a castrating digenean parasite, Labratrema minimus. In spent cockles, MT levels were low (ca. 5 nmol sites g(-1), fresh weight) but slightly higher in parasitized individuals. During gametogenesis, MT synthesis increased in all cockles, but concentrations were lower in parasitized individuals (18 against 27 nmol sites g(-1), fw in unparasitized cockles) in relation with gonad damage by parasites. Therefore, it is suggested that parasite infection in cockles can modulate MT synthesis that could consequently interfere with the response of these protective proteins in case of metal contamination.     

Dredging displaces bottlenose dolphins from an urbanised foraging patch

The exponential growth of the human population and its increasing industrial development often involve large scale modifications of the environment. In the marine context, coastal urbanisation and harbour expansion to accommodate the rising levels of shipping and offshore energy exploitation require dredging to modify the shoreline and sea floor. While the consequences of dredging on invertebrates and fish are relatively well documented, no study has robustly tested the effects on large marine vertebrates. We monitored the attendance of common bottlenose dolphins (Tursiops truncatus) to a recently established urbanised foraging patch, Aberdeen harbour (Scotland), and modelled the effect of dredging operations on site usage. We found that higher intensities of dredging caused the dolphins to spend less time in the harbour, despite high baseline levels of disturbance and the importance of the area as a foraging patch.     

Actinomycetes in New York Harbour sediments and dredging spoil

A large number and variety of species of actinomycetes were isolated from sediment samples collected in New York Harbour and from the New York Bight in the course of studies on the distribution of these bacteria in coastal regions. Isolates recovered from the New York Bight sediment were similar to those from other inshore areas, although the total counts were lower. Larger numbers of actinomycetes were recovered from harbour sediments, as well as dredging spoil samples collected from a capped dump site in the New York Bight. The capping operation was monitored and effects on the microbial populations were detected. Selected genera associated with the dredge spoil were determined to be allochthonous, providing useful microbial markers for the dredged material. Many of the microbial species survive in the dump area, but some appear to be metabolically active, perhaps altering the chemical nature of the dumped material.     

Modelling larval dispersal of the king scallop (Pecten maximus) in the English Channel: examples from the bay of Saint-Brieuc and the bay of Seine

The king scallop (Pecten maximus) is one of the most important benthic species of the English Channel as it constitutes the first fishery in terms of landings in this area. To support strategies of spatial fishery management, we develop a high-resolution biophysical model to study scallop dispersal in two bays along the French coasts of the English Channel (i.e. the bay of Saint-Brieuc and the bay of Seine) and to quantify the relative roles of local hydrodynamic processes, temperature-dependent planktonic larval duration (PLD) and active swimming behaviour (SB). The two bays are chosen for three reasons: (1) the distribution of the scallop stocks in these areas is well known from annual scallop stock surveys, (2) these two bays harbour important fisheries and (3) scallops in these two areas present some differences in terms of reproductive cycle and spawning duration. The English Channel currents and temperature are simulated for 10 years (2000–2010) with the MARS-3D code and then used by the Lagrangian module of MARS-3D to model the transport. Results were analysed in terms of larval distribution at settlement and connectivity rates. While larval transport in the two bays depended both on the tidal residual circulation and the wind-induced currents, the relative role of these two hydrodynamic processes varied among bays. In the bay of Saint-Brieuc, the main patterns of larval dispersal were due to tides, the wind being only a source of variability in the extent of larval patch and the local retention rate. Conversely, in the bay of Seine, wind-induced currents altered both the direction and the extent of larval transport. The main effect of a variable PLD in relation to the thermal history of each larva was to reduce the spread of dispersal and consequently increase the local retention by about 10 % on average. Although swimming behaviour could influence larval dispersal during the first days of the PLD when larvae are mainly located in surface waters, it has a minor role on larval distribution at settlement and retention rates. The analysis of the connectivity between subpopulations within each bay allows identifying the main sources of larvae which depend on both the characteristics of local hydrodynamics and the spatial heterogeneity in the reproductive outputs.     

Tributyltin along the coasts of Corsica (Western Mediterranean): a persistent problem.

Despite optimistic forecasts by various scientists after regulatory measures were taken in the 1980s, coastal tributyltin (TBT) contamination is still a major problem. The present study concerning Corsica (Western Mediterranean) shows that contamination is not limited to harbour areas, but extends along the coast, involving protected nature reserves. The concentrations measured in harbours, which can reach 200 ng TBT l(-1), tend to incriminate both pleasure craft and ferries providing regular service between the island and the continent. Contamination as high as 7 ng TBT l(-1) has been measured in waters of the Scandola nature reserve, which is quite excessive given the no effect concentrations (NOEC) for marine fauna are around 1-2 ng TBT l(-1). The inadequacy of current regulations and their application are a major factor in this situation.     

Evidence for P wave velocity discontinuities at depths greater than 650 km in the mantle

The arrival times of seismic P waves recorded at long lines of portable seismographs deployed on the shield region of central Australia show evidence of breaks in the travel-time curve at epicentral distances near 30, 39 and 43°. These breaks are additional to those at about 20 and 24° (associated with the 400- and 650-km discontinuities) and imply that the P wave velocity structure of the mantle does not increase smoothly in the depth range 650–1100 km, but rather consists of regions of nearly constant velocity separated by small but significant velocity increases at depths of approximately 770, 980 and 1080 km. These conclusions are in agreement with those previously inferred from first and later arrivals at the Warramunga Seismic Array.     

Monitoring the remediation of a near shore waste disposal site in Antarctica using the amphipod Paramoera walkeri and diffusive gradients in thin films (DGTs)

The water quality of a marine embayment (Brown Bay) was monitored during the remediation of an abandoned waste disposal site at Casey Station, East Antarctica, using a combination of biomonitoring and chemical methods. The Antarctic amphipod Paramoera walkeri, in field mesocosms suspended in the water column, was deployed adjacent to the site and at two reference sites for periods of 14 days, repeated three times during the remediation period (December to February). Diffusive gradients in thin film (DGT) samplers were deployed for the same periods to provide estimates of dissolved metals. No difference in mortality of amphipods was observed between Brown Bay and reference sites. There were significant differences, however, in accumulated metal concentrations between amphipods from Brown Bay and reference sites, with greater concentrations of antimony, copper, cadmium, lead, iron and tin at Brown Bay. The melt water/runoff treatment strategy employed for the remediation was successful in preventing acute toxic effects, but water quality was reduced at Brown Bay, where increased metal bioavailability may have been high enough to induce chronic effects in some biota. DGT samplers were less sensitive than amphipods in detecting differences in metal concentrations between sites, indicating that metals bound to suspended particulates were a potentially significant source of contamination.     

Environmental contamination associated with a marine landfill (‘seafill’) beside a coral reef

In Bermuda, bulk waste such as scrap metal, cars, etc., and blocks of cement-stabilized incinerator ash (produced from burning garbage) are disposed of in a foreshore reclamation site, i.e., a seafill. Chemical analyses show that seawater leaching out of the dump regularly exceeds water quality guidelines for Zn and Cu, and that the surrounding sediments are enriched in multiple contaminant classes (metals, polycyclic aromatic hydrocarbons, petroleum hydrocarbons, dioxins and furans, polychlorinated biphenyls and an organochlorine pesticide), i.e., there is a halo of contamination. When compared against biological effects-based sediment quality guidelines (SQGs), numerous sediment samples exceeded the low-range values (where biological effects become possible), and for Hg and Zn exceeded the mid-range value (where they become probable). A few metres away from the edge of the 25 acre dump lies a small coral patch reef, proposed here as most contaminated coral reef in the world.