The effect of density-driven flow on the transport of high-concentration solutes in the hyporheic zone

In this study, both laboratory experiments and numerical simulations were conducted to investigate the effect of density-driven flow on the transport of high-concentration pollutants in the hyporheic zone. The results show that the density gradient can change the flow of pore water and the strong density-driven flow can lead to an unstable flow, which increases the effect of preferential flow and thus causes the appearance of solute fingers in the hyporheic zone. Notably, these solute fingers become more obvious with the increase of depth. The appearance of solute fingers depends on the relative strength of the pumping exchange and density gradient, which are represented by the dimensionless number M* and N* respectively. Finger flows appear near the interface when M^* is less than 0.5 N^*. This study may contribute to better understanding the transport and destination of solutes and thus may provide some insights into the assessment on pollution incidents.     

Interstitial flow through preferential flow paths in the hyporheic zone of the Oberer Seebach,Austria

We investigated interstitial flow velocities in the Oberer Seebach, Austria, with NaCl tracer injections at a sediment depth of 30 cm to estimate the hydraulic conditions experienced by invertebrates inhabiting the hyporheic zone. Flow velocity measured with tracers is taken as travel time of the water along a straight line between injection and sampling points, although the water flows around sediment particles, and thus travels a somewhat longer distance. From sections of stream sediment in which the interstitial spaces were replaced by concrete, we estimated that this difference amounts, on average, to 27% and used this factor to correct the results of our velocity measurements. Corrected interstitial water velocities ranged from 0.01 to 1.32 cm s^-1 and were independent of surface discharge. We also studied spatial flow patterns in the bed sediments with long-term tracer injections. The three-dimensional distribution of tracer concentrations 24 hours after the start of the injection indicated that interstitial water preferentially flows in a complex network of areas of high hydraulic connectivity. Reynolds numbers for flow in the hyporheic pore space ranged from 0.1 to 489, implying that the flow environment varies from laminar up to the zone of transition to turbulent flow. Therefore, invertebrates may have a size-related active choice of areas where either friction drag or pressure drag predominates. The consequence of flow patterns, such as those observed in our study, is that small-scale variability of hydraulic conditions may be an important determinant of the patchy invertebrate distribution in bed sediments.     

Determining long time‐scale hyporheic zone flow paths in Antarctic streams

In the McMurdo Dry Valleys of Antarctica, glaciers are the source of meltwater during the austral summer, and the streams and adjacent hyporheic zones constitute the entire physical watershed; there are no hillslope processes in these systems. Hyporheic zones can extend several metres from each side of the stream, and are up to 70 cm deep, corresponding to a lateral cross‐section as large as 12 m², and water resides in the subsurface year around. In this study, we differentiate between the near‐stream hyporheic zone, which can be characterized with stream tracer experiments, and the extended hyporheic zone, which has a longer time‐scale of exchange. We sampled stream water from Green Creek and from the adjacent saturated alluvium for stable isotopes of D and ¹⁸O to assess the significance and extent of stream‐water exchange between the streams and extended hyporheic zones over long time‐scales (days to weeks). Our results show that water residing in the extended hyporheic zone is much more isotopically enriched (up to 11‰ D and 2·2‰ ¹⁸O) than stream water. This result suggests a long residence time within the extended hyporheic zone, during which fractionation has occurred owing to summer evaporation and winter sublimation of hyporheic water. We found less enriched water in the extended hyporheic zone later in the flow season, suggesting that stream water may be exchanged into and out of this zone, on the time‐scale of weeks to months. The transient storage model OTIS was used to characterize the exchange of stream water with the extended hyporheic zone. Model results yield exchange rates (α) generally an order magnitude lower (10^?5 s^?1) than those determined using stream‐tracer techniques on the same stream. In light of previous studies in these streams, these results suggest that the hyporheic zones in Antarctic streams have near‐stream zones of rapid stream‐water exchange, where ‘fast’ biogeochemical reactions may influence water chemistry, and extended hyporheic zones, in which slower biogeochemical reaction rates may affect stream‐water chemistry at longer time‐scales. Copyright © 2003 John Wiley & Sons, Ltd.     

Colloid transport and distribution in the hyporheic zone

Colloids moving from the stream into the hyporheic zone may have a negative impact on aquatic ecosystems as they are potential contaminants or carriers of contaminants. Moreover, retained colloids in the hyporheic zone could not only reduce the exchange flux between the stream and streambed but also change the conditions of the bed, affecting the habitats for aquatic organisms. Previous studies focused on the exchange flux across the sediment–water interface, but the colloid transport processes and distribution of retained colloids in the streambed have received little attention. We conducted experiments within a laboratory flume to examine these processes in a streambed driven by bedform‐induced hyporheic flow. Retained colloids measured in the bed at the end of the experiments revealed colloid retention mainly in the shallow layer of hyporheic zone (0–5 cm below the interface). The results demonstrated significant effects of particle trapping and settling on the colloid transport and distribution in the streambed. Retention leads to the formation of a colloid‐filled shallow layer in the bed. Particle paths based on model simulations showed that colloid settling in pore water modifies the direction of colloid transport and allows the colloid particles to move more deeply in the bed.     

Imaging hyporheic zone solute transport using electrical resistivity

Traditional characterization of hyporheic processes relies upon modelling observed in‐stream and subsurface breakthrough curves to estimate hyporheic zone size and infer exchange rates. Solute data integrate upstream behaviour and lack spatial coverage, limiting our ability to accurately quantify spatially heterogeneous exchange dynamics. Here, we demonstrate the application of near‐surface electrical resistivity imaging (ERI) methods, coupled with experiments using an electrically conductive stream tracer (dissolved NaCl), to provide in situ imaging of spatial and temporal dynamics of hyporheic exchange. Tracer‐labelled water in the stream enters the hyporheic zone, reducing electrical resistivity in the subsurface (to which subsurface ERI is sensitive). Comparison of background measurements with those recording tracer presence provides distributed characterization of hyporheic area (in this application, ∼0·5 m²). Results demonstrate the first application of ERI for two‐dimensional imaging of stream‐aquifer exchange and hyporheic extent. Future application of this technique will greatly enhance our ability to quantify processes controlling solute transport and fate in hyporheic zones, and provide data necessary to inform more complete numerical models. Copyright © 2010 John Wiley & Sons, Ltd.     

The role of the hyporheic zone across stream networks

Many hyporheic papers state that the hyporheic zone is a critical component of stream ecosystems, and many of these papers focus on the biogeochemical effects of the hyporheic zone on stream solute loads. However, efforts to show such relationships have proven elusive, prompting several questions: Are the effects of the hyporheic zone on stream ecosystems so highly variable in place and time (or among streams) that a consistent relationship should not be expected? Or, is the hyporheic zone less important in stream ecosystems than is commonly expected? These questions were examined using data from existing groundwater modelling studies of hyporheic exchange flow at five sites in a fifth‐order, mountainous stream network. The size of exchange flows, relative to stream discharge (Q_HEF:Q), was large only in very small streams at low discharge (area ≈ 100 ha; Q < 10 l/s). At higher flows (flow exceedance probability > 0·7) and in all larger streams, Q_HEF:Q was small. These data show that biogeochemical processes in the hyporheic zone of small streams can substantially influence the stream's solute load, but these processes become hydrologically constrained at high discharge or in larger streams and rivers. The hyporheic zone may influence stream ecosystems in many ways, however, not just through biogeochemical processes that alter stream solute loads. For example, the hyporheic zone represents a unique habitat for some organisms, with patterns and amounts of upwelling and downwelling water determining the underlying physiochemical environment of the hyporheic zone. Similarly, hyporheic exchange creates distinct patches of downwelling and upwelling. Upwelling environments are of special interest, because upwelling water has the potential to be thermally or chemically distinct from stream water. Consequently, micro‐environmental patches created by hyporheic exchange flows are likely to be important to biological and ecosystem processes, even if their impact on stream solute loads is small. Published in 2011 by John Wiley & Sons, Ltd.     

Control of river stage on the reactive chemistry of the hyporheic zone

We examined the influence of river stage on subsurface hydrology and pore water chemistry within the hyporheic zone of a groundwater‐fed river during the summer baseflow period of 2011. We found river stage and geomorphologic environment to control chemical patterns in the hyporheic zone. At a high river stage, the flux of upwelling water in the shallow sediments (>20 cm) decreased at sample sites in the upper section of our study reach and increased substantially at sites in the lower section. This differential response is attributed to the contrasting geomorphology of these subreaches that affects the rate of the rise and fall of a river stage relative to the subsurface head. At sites where streamward vertical flux decreased, concentration profiles of a conservative environmental tracer suggest surface water infiltration into the riverbed below depths recorded at a low river stage. An increase in vertical flux at sites in the lower subreach is attributed to the movement of lateral subsurface waters originating from the adjacent floodplain. This lateral‐moving water preserved or decreased the vertical extent of the hyporheic mixing zone observed at a low river stage. Downwelling surface water appeared to be responsible for elevated dissolved organic carbon (DOC) and manganese (Mn) concentrations in shallow sediments (0–20 cm); however, lateral subsurface flows were probably important for elevated concentrations of these solutes at deeper levels. Results suggest that DOC delivered to hyporheic sediments during a high river stage from surface water and lateral subsurface sources could enhance heterotrophic microbial activities. Copyright © 2013 John Wiley & Sons, Ltd.     

Spatial variability in the hyporheic zone refugium of temporary streams

A key ecological role hypothesized for the hyporheic zone is as a refugium that promotes survival of benthic invertebrates during adverse conditions in the surface stream. Many studies have investigated use of the hyporheic refugium during hydrological extremes (spates and streambed drying), and recent research has linked an increase in the abundance of benthic invertebrates within hyporheic sediments to increasing biotic interactions during flow recession in a temporary stream. This study examined spatial variability in the refugial capacity of the hyporheic zone in two groundwater-dominated streams in which flow permanence varied over small areas. Two non-insect taxa, Gammarus pulex and Polycelis spp. were common to both streams and were investigated in detail. Hydrological conditions in both streams comprised a four-month period of flow recession and low flows, accompanied by reductions in water depth and wetted width. Consequent declines in submerged benthic habitat availability were associated with increases in population densities of mobile benthic taxa, in particular G. pulex. The reduction in the spatial extent of the hyporheic zone was minimal, and this habitat was therefore a potential refugium from increasing biotic interactions in the benthic sediments. Concurrent increases in the hyporheic abundance and hyporheic proportion of a taxon’s total (benthic + hyporheic) population were considered as evidence of active refugium use. Such evidence was species-specific and site-specific, with refugium use being observed only for G. pulex and at sites dominated by downwelling water. A conceptual model of spatial variability in the refugial capacity of the hyporheic zone during habitat contraction is presented, which highlights the potential importance of the direction of hydrologic exchange.     

Effect of rapidly changing river stage on uranium flux through the hyporheic zone

Measurement of ground water/surface water interaction within the hyporheic zone is increasingly recognized as an important aspect of subsurface contaminant fate and transport. Understanding the interaction between ground water and surface water is critical in developing a complete conceptual model of contaminant transport through the hyporheic zone. At the Hanford Site near Richland, Washington, ground water contaminated with uranium discharges to the Columbia River through the hyporheic zone. Ground water flux varies according to changes in hydraulic gradient caused by fluctuating river stage, which changes in response to operation of dams on the Columbia River. Piezometers and continuous water quality monitoring probes were installed in the hyporheic zone to provide long-term, high-frequency measurement of hydraulic gradient and estimated uranium concentrations. Subsequently, the flux of water and uranium was calculated for each half-hour time period over a 15-month study period. In addition, measurement of water levels in the near-shore unconfined aquifer enhanced the understanding of the relationship between river stage, aquifer elevation, and uranium flux. Changing river stage resulted in fluctuating hydraulic gradient within the hyporheic zone. Further, influx of river water caused lower uranium concentrations as a result of dilution. The methods employed in this study provide a better understanding of the interaction between surface and ground water in a situation with a dynamically varying vertical hydraulic gradient and illustrate how the combination of relatively standard methods can be used to derive an accurate estimation of water and contaminant flux through the hyporheic zone.     

Movement of lateral hyporheic flow between stream and groundwater

The hyporheic zone plays an important role in groundwater and stream water quality protection. To investigate the stream-groundwater interaction mechanisms in the lateral hyporheic zone, this study examined Ma'an Creek in Chongqing during the dry season from December 2015 to April 2016. The water level, water temperature, pH and Cl. concentration in the hyporheic zone and groundwater were monitored in situ. The sediment permeability coefficient, stable isotopes of hydrogen and oxygen and concentration of DOC were analyzed. The results show that the water level changes of hyporheic zone and the movement of hyporheic flow were influenced significantly by the permeability coefficient of sediment. The hyporheic flow approximately10 cm from the stream bank was clearly affected by precipitation infiltration and evapotranspiration. During the study period,the groundwater recharged the stream, and the impact of groundwater on the hyporheic flow gradually decreased with the flow path. The hyporheic flow approximately 30 cm from the stream bank was still mainly affected by groundwater. Approximately10–30 cm from the stream bank, the mixing of groundwater with precipitation and stream water intensified. Due to the sediment properties, moisture accumulated approximately 10 cm from the stream bank and drained into the stream via hyporheic flow, with potential impacts on stream water quality.     

Influence of the hyporheic zone on the phosphorus dynamics of a large gravel‐bed river,Garonne River,France

Phosphorus (P) concentrations in sediments and in surface and interstitial water from three gravel bars in a large river (Garonne River, southern France) were measured daily, downstream of a wastewater treatment plant for a city of 740 000 inhabitants (Toulouse). Measurements were made of vertical hydraulic gradient (VHG), total dissolved phosphorus (TDP), soluble reactive phosphorus (SRP) and total phosphorus (TP) in water and of three extractable forms of phosphorus (water extractable, NaOH extractable and H₂SO₄ extractable) in hyporheic sediments from the gravel bars. Dissolved phosphorus was the major contributor to TP (74–79%) in both interstitial and surface waters on all sampling dates, and in most cases surface water P concentrations were significantly higher than interstitial concentrations. Hyporheic sediment TP concentrations ranged between 269 and 465 µg g^?1 and were highest in fine sediment fractions. Acid‐extractable P, a non‐bioavailable form, represented at least 95% of sediment TP. A positive relationship was observed between VHG and TP in two of the gravel bars, with wells that were strongly downwelling having lower TP concentrations. These results suggest that in downwelling zones, hyporheic sediments can trap surface‐derived dissolved P, and that much of this P becomes stored in refractory particulate forms. Bioavailable P is mainly present in dissolved form and only occupies a small fraction of total P, with particulate P comprising the majority of total P. Copyright © 2009 John Wiley & Sons, Ltd.     

Impacts of river bed gas on the hydraulic and thermal dynamics of the hyporheic zone

Despite the presence of gas in river beds being a well known phenomenon, its potential feedbacks on the hydraulic and thermal dynamics of the hyporheic zone has not been widely studied. This paper explores hypotheses that the presence of accumulated gas impacts the hydraulic and thermal dynamics of a river bed due to changes in specific storage, hydraulic conductivity, effective porosity, and thermal diffusivity. The hypotheses are tested using data analysis and modelling for a study site on the urban River Tame, Birmingham, UK. Gas, predominantly attributed to microbial denitrification, was observed in the river bed up to around 14% by volume, and to at least 0.8 m depth below river bed. Numerical modelling indicates that, by altering the relative hydraulic conductivity distribution, the gas in the river bed leads to an increase of groundwater discharge from the river banks (relative to river bed) by a factor of approximately 2 during river low flow periods. The increased compressible storage of the gas phase in the river bed leads to an increase in the simulated volume of river water invading the river bed within the centre of the channel during storm events. The exchange volume can be more than 30% greater in comparison to that for water saturated conditions. Furthermore, the presence of gas also reduces the water-filled porosity, and so the possible depth of such invading flows may also increase markedly, by more than a factor of 2 in the observed case. Observed diurnal temperature variations within the gaseous river bed at 0.1 and 0.5 m depth are, respectively, around 1.5 and 6 times larger than those predicted for saturated sediments. Annual temperature fluctuations are seen to be enhanced by around 4 to 20% compared to literature values for saturated sediments. The presence of gas may thus alter the bulk thermal properties to such a degree that the use of heat tracer techniques becomes subject to a much greater degree of uncertainty. Although the likely magnitude of thermal and hydraulic changes due to the presence of gas for this site have been demonstrated, further research is needed into the origins of the gas and its spatial and temporal variability to enable quantification of the significance of these changes for chemical attenuation and hyporheic zone biology.     

Temporal and spatial response of hyporheic zone geochemistry to a storm event

Although there has been recent focus on understanding spatial variability in hyporheic zone geochemistry across different morphological units under baseflow conditions, less attention has been paid to temporal responses of hyporheic zone geochemistry to non‐steady‐state conditions. We documented spatial and temporal variability of hyporheic zone geochemistry in response to a large‐scale storm event, Tropical Storm Irene (August 2011), across a pool–riffle–pool sequence along Chittenango Creek in Chittenango, NY, USA. We sampled stream water as well as pore water at 15 cm depth in the streambed at 14 locations across a 30 m reach. Sampling occurred seven times at daily intervals: once during baseflow conditions, once during the rising limb of the storm hydrograph, and five times during the receding limb. Principal component analysis was used to interpret temporal and spatial changes and dominant drivers in stream and pore water geochemistry (n = 111). Results show the majority of spatial variance in hyporheic geochemistry (62%) is driven by differential mixing of stream and ground water in the hyporheic zone. The second largest driver (17%) of hyporheic geochemistry was temporal dilution and enrichment of infiltrating stream water during the storm. Hyporheic sites minimally influenced by discharging groundwater (‘connected’ sites) showed temporal changes in water chemistry in response to the storm event. Connected sites within and upstream of the riffle reflected stream geochemistry throughout the storm, whereas downstream sites showed temporally lagged responses in some conservative and biogeochemically reactive solutes. This suggests temporal changes in hyporheic geochemistry at these locations reflect a combination of changes in infiltrating stream chemistry and hyporheic flowpath length and residence time. The portion of the study area strongly influenced by groundwater discharge increased in size throughout the storm, producing elevated Ca²⁺ and concentrations in the streambed, suggesting zones of localized groundwater inputs expand in response to storms. Copyright © 2013 John Wiley & Sons, Ltd.     

Distribution and species composition of macroinvertebrates in the hyporheic zone of bed sediment

The hyporheic zone is a layer of substrate on a river bed where benthic animals normally live,grow,feed,reproduce,and exist for any portion of their life cycle.The hyporheic zone was studied by samplin...     

Residence time control on hot moments of net nitrate production and uptake in the hyporheic zone

The retention capacity for biologically available nitrogen within streams can be influenced by dynamic hyporheic zone exchange, a process that may act as either a net source or net sink of dissolved nitrogen. Over 5 weeks, nine vertical profiles of streambed chemistry (NO₃^? and NH₄⁺) were collected above two beaver dams along with continuous high‐resolution vertical hyporheic flux data. The results indicate a non‐linear relation of net NO₃^? production followed by net uptake in the hyporheic zone as a function of residence time. This Lagrangian‐based relation is consistent through time and across varied morphology (bars, pools, glides) above the dams, even though biogeochemical and environmental factors varied. The empirical continuum between net NO₃^? production and uptake and residence time is useful for identifying two crucial residence time thresholds: the transition to anaerobic respiration, which corresponds to the time of peak net nitrate production, and the net sink threshold, which is defined by a net uptake in NO₃^? relative to streamwater. Short‐term hyporheic residence time variability at specific locations creates hot moments of net production and uptake, enhancing NO₃^? production as residence times approach the anaerobic threshold, and changing zones of net NO₃^? production to uptake as residence times increase past the net sink threshold. The anaerobic and net sink thresholds for beaver‐influenced streambed morphology occur at much shorter residence times (1.3 h and 2.3 h, respectively) compared to other documented hyporheic systems, and the net sink threshold compares favorably to the lower boundary of the anaerobic threshold determined for this system with the new oxygen Damkohler number. The consistency of the residence time threshold values of NO₃^? cycling in this study, despite environmental variability and disparate morphology, indicates that NO₃^? hot moment dynamics are primarily driven by changes in physical hydrology and associated residence times. Copyright © 2013 John Wiley & Sons, Ltd.     

The effects of discharge and slope on hyporheic flow in step‐pool morphologies

This paper focuses on surface–subsurface water exchange in a steep coarse‐bedded stream with a step‐pool morphology. We use both flume experiments and numerical modelling to investigate the influence of stream discharge, channel slope and sediment hydraulic conductivity on hyporheic exchange. The model step‐pool reach, whose topography is scaled from a natural river, consists of three step‐pool units with 0.1‐m step heights, discharges ranging between base and over‐bankfull flows (scaled values of 0.3–4.5 l/s) and slopes of 4% and 8%. Results indicate that the deepest hyporheic flow occurs with the steeper slope and at moderate discharges and that downwelling fluxes at the base of steps are highest at the largest stream discharges. In contrast to findings in a pool‐riffle morphology, those in this study show that steep slopes cause deeper surface–subsurface exchanges than gentle slopes. Numerical simulation results show that the portion of the hyporheic zone influenced by surface water temperature increases with sediment hydraulic conductivity. These experiments and numerical simulations emphasize the importance of topography, sediment permeability and roughness elements along the channel surface in governing the locations and magnitude of downwelling fluxes and hyporheic exchange. Our results show that hyporheic zones in these steep streams are thicker than previously expected by extending the results from streams with pool‐riffle bed forms. Copyright © 2014 John Wiley & Sons, Ltd.     

Influence of macroinvertebrates on physico‐chemical and microbial processes in hyporheic sediments

The objective of this study was to measure the effects of invertebrates on the physical characteristics and microbial processes in hyporheic sediments. We investigated the impacts of an assemblage of three taxa (asellids, chironomid larvae, and tubificid worms) on sediment distribution, water fluxes, sediment organic carbon, biofilm (attached bacteria) characteristics, and O₂, dissolved organic carbon NO₃^?, NO₂^?, and NH₄⁺ concentrations in slow filtration sand–gravel columns. The results showed that invertebrates clearly modified the distribution of particles in the sediment column, probably because of the structures (tubes, macropores, and faecal pellets) produced by the three taxa in the sediment. Our assessment of water fluxes indicated that invertebrate activities led to an increase in the porosity of the sediment columns. In addition, aerobic (O₂ consumption) and anaerobic (denitrification and fermentative decomposition of organic matter) microbial processes occurring in the sediment were stimulated in the presence of invertebrates. Finally, the present study demonstrates that invertebrates can act as ecosystem engineers in heterogeneous sediments that are under the influence of an advective flux of water. The solute residence time increased in columns containing the faunal assemblage. Micro‐organisms used more dissolved organic matter and nutrients in the presence of invertebrates because invertebrate activities increased the contact between the biofilm and water. We conclude that engineering by invertebrates in natural conditions modifies characteristics of the hyporheic zone and thereby enhances both the porosity of the sediment and the solute transport across the benthic interface. Copyright © 2002 John Wiley & Sons, Ltd.     

High frequency stream bed mobility of a low‐gradient agricultural stream with implications on the hyporheic zone

Little Kickapoo Creek (LKC), a low‐gradient stream, mobilizes its streambed–fundamentally altering its near‐surface hyporheic zone–more frequently than do higher‐gradient mountain and karst streams. LKC streambed mobility was assessed through streambed surveys, sediment sampling, and theoretical calculations comparing basal shear stress (τ_b) with critical shear stress (τ_c). Baseflow τ_b is capable of entraining a d₅₀ particle; bankfull flow could entrain a 51·2 mm particle. No particle that large occurs in the top 30 cm of the substrate, suggesting that the top 30 cm of the substrate is mobilized and redistributed during bankfull events. Bankfull events occur on average every 7·6 months; flows capable of entraining d₅₀ and d₈₅ particles occur on average every 0·85 and 2·1 months, respectively. Streambed surveys verify streambed mobility at conditions below bankfull. While higher gradient streams have higher potential energy than LKC, they achieve streambed‐mobilization thresholds less frequently. Heterogeneous sediment redistribution creates an environment where substrate hydraulic conductivity (K) varies over four orders of magnitude. The frequency and magnitude of the substrate entrainment has implications on hyporheic zone function in fluid, solute and thermal transport models, interpretations of hyporheic zone stability, and understanding of LKC's aquatic ecosystem. Copyright © 2008 John Wiley & Sons, Ltd.     

Salinity‐induced increase of the hydraulic conductivity in the hyporheic zone of coastal wetlands

In coastal zones globally, salinization is rapidly taking place due to the combined effects of sea level rise, land subsidence, altered hydrology, and climate change. Although increased salinity levels are known to have a great impact on both biogeochemical and hydrological processes in aquatic sediments, only few studies have included both types of processes and their potential interactions. In the present paper, we used a controlled 3‐year experimental mesocosm approach to test salinity induced interactions and discuss mechanisms explaining the observed hydrological changes. Surface water salinity was experimentally increased from 14 to 140 mmol Cl per L (0.9 and 9 PSU) by adding sea salt which increased pore water salinity but also increased sulfate reduction rates, leading to higher sulfide, and lower methane concentrations. By analyzing slug test data with different slug test analysis methods, we were able to show that hydraulic conductivity of the hyporheic zone increased 2.8 times by salinization. Based on our hydrological and biogeochemical measurements, we conclude that the combination of pore dilation and decreased methane production rates were major controls on the observed increase in hydraulic conductivity. The slug test analysis method comparison allowed to conclude that the adjusted Bouwer and Rice method results in the most reliable estimate of the hydraulic conductivity for hyporheic zones. Our work shows that both physical and biogeochemical processes are vital to explain and predict hydrological changes related to the salinization of hyporheic zones in coastal wetlands and provides a robust methodological approach for doing so.