In Situ Biological Contamination Studies of the Moon: Implications for Planetary Protection and Life Detection Missions

NASA and ESA have outlined visions for solar system exploration that will include a series of lunar robotic precursor missions to prepare for, and support a human return to the Moon, and future human exploration of Mars and other destinations, including possibly asteroids. One of the guiding principles for exploration is to pursue compelling scientific questions about the origin and evolution of life. The search for life on objects such as Mars will require careful operations, and that all systems be sufficiently cleaned and sterilized prior to launch to ensure that the scientific integrity of extraterrestrial samples is not jeopardized by terrestrial organic contamination. Under the Committee on Space Research’s (COSPAR’s) current planetary protection policy for the Moon, no sterilization procedures are required for outbound lunar spacecraft, nor is there a different planetary protection category for human missions, although preliminary COSPAR policy guidelines for human missions to Mars have been developed. Future in situ investigations of a variety of locations on the Moon by highly sensitive instruments designed to search for biologically derived organic compounds would help assess the contamination of the Moon by lunar spacecraft. These studies could also provide valuable “ground truth” data for Mars sample return missions and help define planetary protection requirements for future Mars bound spacecraft carrying life detection experiments. In addition, studies of the impact of terrestrial contamination of the lunar surface by the Apollo astronauts could provide valuable data to help refine future Mars surface exploration plans for a human mission to Mars.     

LunarEX—a proposal to cosmic vision

While the surface missions to the Moon of the 1970s achieved a great deal, scientifically much was also left unresolved. The recent plethora of lunar missions (flown or proposed) reflects a resurgence in interest in the Moon, not only in its own right, but also as a record of the early solar system including the formation of the Earth. Results from recent orbiter missions have shown evidence of ice or at least hydrogen within shadowed craters at the lunar poles.     

Water and other volatiles on the moon: A review

This paper presents a review of research findings on the various forms of water on the Moon. First, this is the water of the Moon’s interior, which has been detected by sensitive mass spectrometric analysis of basaltic glasses delivered by the Apollo 15 and Apollo 17 missions. The previous concepts that lunar magmas are completely dehydrated have been disproved. Second, this is H₂O and/or OH in a thin layer (a few upper millimeters) of the lunar regolith, which is likely a result of bombardment of the oxygen contained in the lunar regolith with solar wind protons. This form of water is highly unstable and quite easily escapes from the surface, possibly being one of the sources of the water ice reservoirs at the Moon’s poles. Third, this is water ice associated with other frozen gases in cold traps at the lunar poles. Its possible sources are impacts of comets and meteorites, the release of gas from the Moon’s interior, and solar wind protons. The ice trapped at the lunar polars could be of practical interest for further exploration of the Moon.     

Tektites - volcanic ejecta from the moon?

The problem of the origin of the enigmatic tektites is still unsolved. The two leading hypotheses - viz., ejecta from terrestrial impacts, and ejecta from lunar volcanoes or lunar impacts, each encounters serious difficulties. The former has ballistic and water content difficulties, while the latter has some compositional difficulties, especially in the trace elements, as determined from the returned samples. It is possible that the latter problem may be met through lunar volcanic ejecta from sites suggesting more differentiation than the majority of the Moon. That such features may exist is suggested from the identity of some granitic material in the returned rocks and soil samples implying fairly sizable source regions on the Moon. The rare terrestrial strewn tektite fields require restrictive ballistic trajectories from the Moon. Calculations reveal that ellipses of varying, decreasing sizes which depend on velocity of vertical ejection from which ejecta will intersect the earth at low-entrance angles occur on the nearside of the Moon. Reasonable velocities were chosen (2.55 to 3.0 km s^?1) and these ellipses circumscribe areas with longitudes between 30 and 50° east and latitudes between 7° north and south of the Moon's equator. These areas were searched for evidence of volcanism. As tektites have compositions ranging from acidic (major tektites) to basic (micro-tektites) contents of silica (SiO₂) both acidic and basic volcanic features were sought. Since tektites range in age from about 30 million to 700000 yr old, they imply recent volcanism. Lunar Transient Phenomena (LTP) and data from various Apollo missions indicate that mild internal activity may still be occurring on the Moon. LTP sites are logical sources to investigate, of which four occur within the above delimited regions. These and their surroundings were examined and a number of possible explosive volcanism sites were found. These sites are identified and discussed after a review of the manifestations found from the various kinds of terrestrial volcanism for which lunar counterparts were sought.     

今后几年的月球探测和月球科学

近年来月球探测已经进入了一个全新的时代。特别是 1 990年以来 ,多个月球探测计划已经被成功实现 ,而且另外还有多个探测计划也在准备当中 ,并将在未来的几年内发射升空。在这种背景之下 ,中国的航天机构和有关的科学家也开始积极酝酿和开发自己的月球探测计划。这些月球探测计划将利用卫星上搭载的各种仪器探测和测量月球的地质和地理特性、化学成分和矿物组成、月球物理学特征以及包含地球大气在内的地月空间环境和行星际空间环境 ;进一步研究月球的起源和演化 ,探明月面环境 ,研究太阳等离子体物理 ,提供月面天文台和月面长期科研基地的候选地址 ,调查月球上的可利用资源 ,为将来开发月球提供充实的背景资料。参与新一轮的月球探测同样也为中国天文学研究带来了新的机会。     

INSTRUMENTATION FOR GEOLOGICAL FIELD WORK ON THE MOON

A human return to the Moon will require that astronauts are well equipped with instrumentation to aid their investigations during geological field work. Two instruments are described in detail. The first is a portable X-ray Spectrometer, which can provide rapid geochemical analyses of rocks and soils, identify lunar resources and aid selection of samples for return to Earth. The second instrument is the Geological and Radiation environment package (GEORAD). This is an instrument package, mounted on a rover, to perform in-situ measurements on the lunar surface. It can be used for bulk geochemical measurements of rocks and soils (particularly identifying KREEP-enriched rocks), prospect for ice in shadowed areas of craters at the poles and characterise the lunar radiation environment.     

Scientific rationale for the D-CIXS X-ray spectrometer on board ESA's SMART-1 mission to the Moon

The D-CIXS X-ray spectrometer on ESA's SMART-1 mission will provide the first global coverage of the lunar surface in X-rays, providing absolute measurements of elemental abundances. The instrument will be able to detect elemental Fe, Mg, Al and Si under normal solar conditions and several other elements during solar flare events. These data will allow for advances in several areas of lunar science, including an improved estimate of the bulk composition of the Moon, detailed observations of the lateral and vertical nature of the crust, chemical observations of the maria, investigations into the lunar regolith, and mapping of potential lunar resources. In combination with information to be obtained by the other instruments on SMART-1 and the data already provided by the Clementine and Lunar Prospector missions, this information will allow for a more detailed look at some of the fundamental questions that remain regarding the origin and evolution of the Moon.     

Preface: The Chang'e-3 lander and rover mission to the Moon

The Chang'e-3(CE-3) lander and rover mission to the Moon was an intermediate step in China's lunar exploration program, which will be followed by a sample return mission. The lander was equipped with a number of remote-sensing instruments including a pair of cameras(Landing Camera and Terrain Camera) for recording the landing process and surveying terrain, an extreme ultraviolet camera for monitoring activities in the Earth's plasmasphere, and a first-ever Moon-based ultraviolet telescope for astronomical observations. The Yutu rover successfully carried out close-up observations with the Panoramic Camera, mineralogical investigations with the VIS-NIR Imaging Spectrometer, study of elemental abundances with the Active Particle-induced X-ray Spectrometer, and pioneering measurements of the lunar subsurface with Lunar Penetrating Radar. This special issue provides a collection of key information on the instrumental designs, calibration methods and data processing procedures used by these experiments with a perspective of facilitating further analyses of scientific data from CE-3 in preparation for future missions.     

Lunar dust and lunar simulant activation and monitoring

Abstract— NASA plans to resume human exploration of the Moon in the next decade. One of the pressing concerns is the effect that lunar dust (the fraction of the lunar regolith <20 μm in diameter) will have on systems, both human and mechanical, due to the fact that various problems were caused by dust during the Apollo missions. The loss of vacuum integrity in the lunar sample containers during the Apollo era ensured that the present lunar samples are not in the same condition as they were on the Moon; they have been passivated by oxygen and water vapor. To mitigate the harmful effects of lunar dust on humans, methods of “reactivating” the dust must be developed for experimentation, and, ideally, it should be possible to monitor the level of activity to determine methods of deactivating the dust in future lunar habitats. Here we present results demonstrating that simple grinding, as a simple analog to micrometeorite crushing, is apable of substantially activating lunar dust and lunar simulant, and it is possible to determine the level of chemical activity by monitoring the ability of the dust to produce hydroxyl radicals in aqueous solution. Comparisons between ground samples of lunar dust, lunar simulant, and quartz reveal that ground lunar dust is capable of producing over three times the amount of hydroxyl radicals as lunar simulant and an order of magnitude more than ground quartz.     

Experimental constraints on the solidification of a hydrous lunar magma ocean

The identification of hydrogen in a range of lunar samples and the similarity of its abundance and isotopic composition with terrestrial values suggest that water could have been present in the Moon since its formation. To quantify the effect of water on early lunar differentiation, we present new analyses of a high‐pressure, high‐temperature experimental study designed to model the mineralogical and geochemical evolution of the solidification material equivalent to 700 km deep lunar magma oceans first reported in Lin et al. (2017a). We also performed additional experiments to better quantify water contents in the run products. Water contents in the melt phases in hydrous run products spanning a range of crystallization steps were quantified directly using a secondary ion mass spectrometry (SIMS). Results suggest that a significant but constant proportion (68 ± 5%) of the hydrogen originally added to the experiments was lost from the starting material independent of run conditions and run duration. The volume of plagioclase formed during our crystallization experiments can be combined with the measured water contents and the observed crustal thickness on the Moon to provide an updated lunar interior hygrometer. Our data suggest that at least 45–354 ppm H₂O equivalent was present in the Moon at the time of crust formation. These estimates confirm the inference of Lin et al. (2017a) that the Moon was wet during its magma ocean stage, with corrected absolute water contents now comparable to estimates derived from the water content in a range of lunar samples.     

Laboratory studies on seismic and electrical properties of the moon

Laboratory measurements of seismic wave velocities and electrical properties of Apollo lunar samples and similar material of terrestrial origin are discussed in this paper. Measurements of the electrical properties show that in the frequency range above a few hundred Hz the outer region of the Moon may be considered as a low loss dielectric. This observation supports a longstanding speculation that dry, powdered rocks in which the dielectric loss tangent is frequency-independent over a wide range of frequency are present in the uppermost lunar surface layers. The surface layers of the Moon are likely to have an extremely low electrical conductivity. Thus future electromagnetic probing of the Moon to a few hundred kilometer depth is possible in the few kHz frequency range. Based on ultrasonic experiments with pressure as a variable, we next present the elastic constants and equations of state of lunar materials and characteristic dispersion of seismic wave velocities of the Moon. We find thatP andS wave velocities increase sharply within the first 30 km depth and then level off gradually. Combining this observation with lunar seismic and geophone data, we believe that the first 30 km of the Moon may be interpreted as a scattering region. If H₂O exists on the Moon, H₂O may occur at some shallow depth beneath the outermost surface layer in solid ice interlocking cracks and pores and mineral grains. The rocks in this permafrost state have relatively low seismic velocity and highQ. If permafrost does exist, we would expect a wide range of electrical conductivity and dielectric constant. Future electromagnetic probing of the Moon should yield very usefull information on the physical state of the lunar interior; when this electrical information is combined with the seismic information, we should learn much more about the internal constitution and the state of the Moon than is known today.     

Lunar Beagle and Lunar Astrobiology

The study of the elements and molecules of astrobiological interest on the Moon can be made with the Gas Analysis Package (GAP) and associated instruments developed for the Beagle 2 Mars Express Payload. The permanently shadowed polar regions of the Moon may offer a unique location for the “cold-trapping” of the light elements (i.e. H, C, N, O, etc.) and their simple compounds. Studies of the returned lunar samples have shown that lunar materials have undergone irradiation with the solar wind and adsorb volatiles from possible cometary and micrometeoroid impacts. The Beagle 2’s analytical instrument package including the sample processing facility and the GAP mass spectrometer can provide vital isotopic information that can distinguish whether the lunar volatiles are indigenous to the moon, solar wind derived, cometary in origin or from meteoroids impacting on the Moon. As future Lunar Landers are being considered, the suite of instruments developed for the Mars Beagle 2 lander can be consider as the baseline for any lunar volatile or resource instrument package.     

An intrinsic volatility scale relevant to the Earth and Moon and the status of water in the Moon

The notion of a dry Moon has recently been challenged by the discovery of high water contents in lunar apatites and in melt inclusions within olivine crystals from two pyroclastic glasses. The highest and most compelling water contents were found in pyroclastic glasses that are not very common on the lunar surface. To obtain more representative constraints on the volatile content of the lunar interior, we measured the Zn content, a moderately volatile element, of mineral and rock fragments in lunar soils collected during Apollo missions. We here confirm that the Moon is significantly more depleted in Zn than the Earth. Combining Zn with existing K and Rb data on similar rocks allows us to anchor a new volatility scale based on the bond energy of nonsiderophile elements in their condensed phases. Extrapolating the volatility curve to H shows that the bulk of the lunar interior must be dry (≤1 ppm). This contrasts with the water content of the mantle sources of pyroclastic glasses, inferred to contain up to approximately 40 ppm water based on H₂O/Ce ratios. These observations are best reconciled if the pyroclastic glasses derive from localized water‐rich heterogeneities in a dominantly dry lunar interior. We argue that, although late addition of 0.015% of a chondritic veneer to the Moon seems required to explain the abundance of platinum group elements (Day et al. 2007), the volatile content of the added material was clearly heterogeneous.     

‘Direct’ evidence for water (H2O) in the sunlit lunar ambience from CHACE on MIP of Chandrayaan I

Direct detection of water in its vapour phase in the tenuous lunar environment through in situ measurements carried out by the Chandra’s Altitudinal Composition Explorer (CHACE) payload, onboard the Moon Impact Probe (MIP) of Chandrayaan I mission vindicates the presence of water on the surface of the moon in form of ice at higher lunar latitudes inferred from IR absorption spectroscopy, (especially that of OH), by the Moon Mineralogy Mapper (M³) of Chandrayaan I. The quadrupole mass spectrometer based payload, CHACE, sampled the lunar neutral atmosphere every 4 s with a broad latitudinal (∼40°N to 90°S, with a resolution of ∼0.1°) and altitudinal (from 98 km up to impact on the lunar surface with a resolution of ∼0.25 km) coverage in the sunlit side of the moon for the first time. These two (CHACE and M³) complementary experiments are shown to collectively provide unambiguous signatures for the distribution of water in solid and gaseous phases in Earth’s moon.     

The lunar gravity mission MAGIA: preliminary design and performances

The importance of an accurate model of the Moon gravity field has been assessed for future navigation missions orbiting and/or landing on the Moon, in order to use our natural satellite as an intermediate base for next solar system observations and exploration as well as for lunar resources mapping and exploitation. One of the main scientific goals of MAGIA mission, whose Phase A study has been recently funded by the Italian Space Agency (ASI), is the mapping of lunar gravitational anomalies, and in particular those on the hidden side of the Moon, with an accuracy of 1 mGal RMS at lunar surface in the global solution of the gravitational field up to degree and order 80. MAGIA gravimetric experiment is performed into two phases: the first one, along which the main satellite shall perform remote sensing of the Moon surface, foresees the use of Precise Orbit Determination (POD) data available from ground tracking of the main satellite for the determination of the long wavelength components of gravitational field. Improvement in the accuracy of POD results are expected by the use of ISA, the Italian accelerometer on board the main satellite. Additional gravitational data from recent missions, like Kaguya/Selene, could be used in order to enhance the accuracy of such results. In the second phase the medium/short wavelength components of gravitational field shall be obtained through a low-to-low (GRACE-like) Satellite-to-Satellite Tracking (SST) experiment. POD data shall be acquired during the whole mission duration, while the SST data shall be available after the remote sensing phase, when the sub-satellite shall be released from the main one and both satellites shall be left in a free-fall dynamics in the gravity field of the Moon. SST range-rate data between the two satellites shall be measured through an inter-satellite link with accuracy compliant with current state of art space qualified technology. SST processing and gravitational anomalies retrieval shall benefit from a second ISA accelerometer on the sub-satellite in order to decouple lunar gravitational signal from other accelerations. Experiment performance analysis shows that the stated scientific requirements can be achieved with a low mass and low cost sub-satellite, with a SST gravimetric mission of just few months.     

Capability of the penetrator seismometer system for lunar seismic event observation

We developed a seismometer system for a hard landing “penetrator” probe in the course of the former Japanese LUNAR-A project to deploy new seismic stations on the Moon. The penetrator seismometer system (PSS) consists of two short-period sensor components, a two-axis gimbal mechanism for orientation, and measurement electronics. To carry out seismic observations on the Moon using the penetrator, the seismometer system has to function properly in a lunar environment after a hard landing (impact acceleration of about 8000 G), and requires a signal-to-noise ratio to detect lunar seismic events. We evaluated whether the PSS could satisfactorily observe seismic events on the Moon by investigating the frequency response, noise level, and response to ground motion of our instrument in a simulated lunar environment after a simulated impact test. Our results indicate that the newly developed seismometer system can function properly after impact and is sensitive enough to detect seismic events on the Moon. Using this PSS, new seismic data from the Moon can be obtained during future lunar missions.     

Benefits of the Proposed Magia Mission for Lunar Geology

Age of geological units, surface mineralogical composition, volcanism, tectonics and cratering are major keys for unravelling the geodynamic and geological history of a planet. Thanks to the extensive exploration of the 1960s and 1970s and the compositional mapping of the 1990s missions (Galileo, Clementine and Luna Prospector), the Moon has a unique geological dataset among the extraterrestrial Solar System bodies. The recent and on-going missions, along with the future plans for lunar exploration, will together acquire an extraordinary amount of data. This should provide a solid basis to meet broad objectives like the constraints on the heterogeneity of Lunar composition and the presence of water deposits, the understanding of volcanic and tectonic evolution as well as more specific issues such as the genetic classification of volcanic domes, origin of the dark-halos craters, lava flow emplacement mechanisms, and the kinematics and deformational styles of tectonic structures. The Italian small mission MAGIA (Missione Altimetrica Gravimetrica geochImica lunAre) will be equipped with an integrated context camera and imaging spectrometer, a high resolution camera and a radar altimeter. The spatial and spectral resolution of these instruments will provide data products complementing past and ongoing Lunar mission data, particularly for the polar regions where a full resolution coverage is planned. A general review of some still unanswered questions on lunar surface composition, cold traps, volcanism, tectonics and cratering records is presented here in order to illustrate the potential contribution of MAGIA to these subjects.     

Comment on: Constraints on the depth and variability of the lunar regolith,by B. B. Wilcox,M. S. Robinson,P. C. Thomas,and B. R. Hawke

Abstract— Any permanent presence on the Moon will require use of materials from the lunar regolith, the surface soil layer on the Moon. Thus, knowledge of the thickness of the lunar regolith is essential. It has been proposed that crater counts obtained from high Sun angle photography give larger estimates of impact crater equilibrium diameters than for low Sun angle photography, and thus deeper estimates of lunar surface regolith than were previously made using crater morphology, size of blocky rimmed craters, and equilibrium diameters determined on low Sun angle images. The purpose of this comment is to evaluate this result as a means of resolving this important question before planning for future lunar missions is undertaken     

Observations of water vapor ions at the lunar surface

The Apollo 14 Suprathermal Ion Detector Experiment observed a series of bursts of 48.6 eV water vapor ions at the lunar surface during a 14-h period on March 7, 1971. The maximum flux observed was 10⁸ ions cm^–2 s^–1 sr^–1. These ions were also observed at Apollo 12, 183 km to the west. Evaluation of specific artificial sources including the Apollo missions and the Russian Lunokhod leads to the conclusion that the water vapor did not come from a man-made source. Natural sources exogenous to the Moon such as comets and the solar wind are also found to be inadequate to explain the observed fluxes. Consequently, these water vapor ions appear to be of lunar origin.Paper dedicated to Prof. Harold C. Urey on the occasion of his 80th birthday on 29 April 1973.     

Solar wind contribution to surficial lunar water: Laboratory investigations

Remote infrared spectroscopic measurements have recently re-opened the possibility that water is present on the surface of the Moon. Analyses of infrared absorption spectra obtained by three independent space instruments have identified water and hydroxyl (-OH) absorption bands at ∼3 μm within the lunar surface. These reports are surprising since there are many mechanisms that can remove water but no clear mechanism for replenishment. One hypothesis, based on the spatial distribution of the -OH signal, is that water is formed by the interaction of the solar wind with silicates and other oxides in the lunar basalt. To test this hypothesis, we have performed a series of laboratory simulations that examine the effect of proton irradiation on two minerals: anorthite and ilmenite. Bi-directional infrared reflection absorption spectra do not show any discernable enhancement of infrared absorption in the 3 μm spectral region following 1 or 100 keV proton irradiation at fluences between 10¹⁶ and 10¹⁸ ions cm⁻². In fact, the post-irradiation spectra are characterized by a decrease in the residual O-H band within both minerals. Similarly, secondary ion mass spectrometry shows a decrease rather than an increase of the water group ions following proton bombardment of ilmenite. The absence of significant formation of either -OH or H₂O is ascribed to the preferential depletion of oxygen by sputtering during proton irradiation, which is confirmed by post-irradiation surface analysis using X-ray photoelectron spectroscopy measurements. Our results provide no evidence to support the formation of H₂O in the lunar regolith via implantation of solar wind protons as a mechanism responsible for the significant O-H absorption in recent spacecraft data. We determine an upper limit for the production of surficial -OH on the lunar surface by solar wind irradiation to be 0.5% (absorption depth).