Thermolability of dehydrogenases from a psychrophilic marine bacterium

The heat lability of succinic dehydrogenase, malic dehydrogenase and lactate dehydrogenase in cell-free extracts ofVibrio marinus MP-1 grown at 15C was compared for the tris buffer suspended enzymes and the NaCl suspended enzymes. The tris buffer suspended enzymes lost 50 percent activity at low thermal exposure between 5C and 27C. No activity was evident on thermal exposure between 32C and 39C. The enzyme extracts suspended in NaCl at 36 % were more heat stable, losing 50 percent activity after exposure between 16C and 40C. The enzymes lost all activity on thermal exposure between 33C and 42C in the presence of NaCl.     

Heat balance of the upper ocean under a land and sea breeze in Sagami Bay in summer

The heat balance of the upper ocean under a land and sea breeze was investigated based on observations of sea water temperature in the upper 300 m layer and heat flux across the sea surface at a fixed station in Sagami Bay (3510N, 13925E) during two periods of two days in August 1980 and three days in August 1981. During both periods, a typical land and sea breeze of 4–6 m sec^–1 at maximum prevailed in the observation area. Large diurnal variation of sea surface temperature with a maximum peak around noon LST was observed during both periods (the daily value of the range was 0.9C and 2.5C in 1980, and 1.2C, 1.5C and 1.7C in 1981). It was found that these large temperature variations were caused by diurnal variation of the wind speed which dropped to 0–3 m sec^–1 at noon when the strongest insolation (–270 Wm^–2) penetrated the sea and at midnight in association with alternations of the land breeze and the sea breeze. On the other hand, vertical mixing of the sea water caused by the wind stress and/or convection due to cooling at night extended down only to the surface 10 m layer. Horizontal heat advection was negligibly small. Therefore the local time change of the heat content in the upper 10 m water column was affected mainly by the heat flux across the air-sea interface which was estimated from data on radiation fluxes measured directly on board and latent and sensible heat fluxes calculated by the aerodynamic bulk method. The water temperature below the 10 m layer also varied with time and the temperature variation in the thermocline (20–50 m depth) was frequently larger than that of the sea surface temperature. However, the variation in the upper 10 m layer was little influenced by that below the layer.     

Differential sorption of organic matter by various sized sediment particles in recent sediment from the bering sea

A recent unconsolidated marine sediment collected from the Aleutian basin of the Bering Sea (5702.9N, 17657.4W) was separated into eight size fractions by sieving. Each of the fractions was analyzed for density, organic carbon, total nitrogen and various organic materials. A detailed analysis of the correlation of organic carbon and total nitrogen to the mean diameter of the particles separated, indicated that the surface sorption processes of organic matter were the determinative factor controlling the concentration of organic carbon and total nitrogen on silt and clay particles of the sediments.Selective sorption of amino acids was found to occur in the 8–20 and 5–8m. fractions of the sediment. This is assumed to be due to their surface active property.     

The heterotrophic activity for amino acids,glucose and acetate in Antarctic waters

The kinetic approach ofWright andHobbie (1966) was used to determine the heterotrophic potential of Antarctic waters. Variations with depth and geographical location are discussed. Temperature studies indicate the psychrophilic nature of the microbial population. In samples having environmental temperatures of approximately –1C, significant activities were recorded at the lowest incubation temperatures tested (–3C). Activities increased with increasing incubation temperatures up to +3C but were somewhat depressed at +10C and above. By testing a wide range of substrates, qualitative differences were noted in the microbial populations of different areas.Highest rates of uptake were found in the euphotic zone whereas V_max values of samples from depths of 500 m and below were in most cases unmeasurable.Published as Technical Paper No. 4259, Oregon Agricultural Experiment Station.     

Moored instrument observations in the Kuroshio south of Kyushu

In each of the (fiscal) years 1979–1982, multilayer current measurements by means of moored instruments were carried out in the Kuroshio off Tanegashima Island, south of Kyushu, with particular interest in the baroclinic layer (0–1,000 m) of the intense current. Most of the moored instruments were in position for more than 30 days, the maximum duration being 145 days. This paper describes some noticeable features of the mean and fluctuation fields in the surface Kuroshio as inferred from the the new data sets.Vertical profiles of horizontal velocities show that the eastward current of the Kuroshio in the region is confined to the upper 600 m on average and below this level the mean current is directed to the southwest along the local isobaths. This is believed to be the first observation of the level of no motion in this region. It is argued that the indicated level of no motion would lead to much smaller values for the Kuroshio transport than previous values referred to the 1,000 m level — for example, earlier estimates by Nitani (1972).The fluctuation field in the upper intense current appears to be dominated by an organized motion with a time scale of about 30 days. This can be seen clearly in the time variations of water temperature and local volume transport, but less clearly in the velocity records. The horizontal transfer components of momentum and heat are calculated and it is suggested that the transverse momentum transfer, UV tends to be negative (offshore) in the region of anticyclonic shear of the upper Kuroshio; no evidence for a negative eddy viscosity effect is found.     

Observations of upwelling around the Izu Peninsula,Japan: May 1982

Hydrographic observations between the Izu Peninsula and Oshima Island, Japan, in May 1982, showed upwelling around the tip of the Peninsula and possibly also in the lee of Oshima Island. The upwelling introduced water as cold as 18 C with nitrate concentrations of 3M to the surface. Temperature/salinity analyses indicated that the upwelled water was of Kuroshio characteristics. Slightly fresher water was advected out of Sagami Bay in a coastal counter current.Contribution number 470 from the Shimoda Marine Research Center, University of Tsukuba.     

Rates of net assimilation and respiration of amino acids by marine bacteria

The selectivity of amino acid assimilation by marine bacteria was examined using seven kinds of¹⁴C-amino acids and the acid hydrolysate of¹⁴C-labelled proteins. It was found that the net assimilation and respiration by marine bacteria followed MICHAELIS-MENTEN kinetics for all of amino acids used in our experiments. Maximum velocities of amino acids were 0.01 to 0.19g carbon/hour per 2×10⁷ cells for net assimilation and less than 0.18g carbon/hour per 2×10⁷ cells for respiration at 20C. The velocity of gross assimilation was found with the following order: phenylalanine>valine, glutamic acid>serine, arginine>tryptophan>glycine. The assimilation velocities of amino acids in these laboratory works showed almost the same order as those in field experiments. The assimilation velocity of an amino acid was influenced by coexisting another amino acids or glucose. The assimilation velocity in lower substrate range of amino acids was directly proportional to the number of bacterial cells in the range from 6×10² to 3×10⁴ cells per ml. No linear relation between the assimilation velocity of amino acids and reciprocal of absolute temperature was found, but a marked bending was observed at 15 to 20C. The velocity at the optimum temperature was three to six times of that at 5C.     

Cold water areas in the vicinity of the shoal,Kokushô-sone

By using data obtained at about 120 XBT stations, cold water regions in the vicinity of the shoal, Kokushô-sone (30°00N, 128°30E), which is located in the current zone of the Kuroshio in the East China Sea, were investigated.The temperature cross-sections obtained were compared with corresponding cross-sections obtained from the four former cruises which were already reported. On the present cruise forced upwelling area was found along the south slope of the shoal, instead of the north slope as was found on the former cruises.The area of the cold water region found along the south slope tends to decrease with decrease in depth, and at depths shallower than 250 m the cold water region extends northward passing the shoal. The area at a depth of 400 m is comparable to that of the shoal itself, and is about 35 km².Physical parameters and their scales which seem to be related to the dynamics near the shoal are given in the Appendix.     

Studies on the dissolution of diatomaceous silica as a function of heating

Diatomaceous silica, pretreated at various temperatures between 125 and 900C, was subjected to dissolution experiments and was analyzed using X-ray diffraction and infrared spectra. The diatomaceous silica preheated up to 900C, dissolved throughly into the solution of 2 per cent Na₂CO₃. On the digestion in the solution of 2 per cent Na₂SO₄, the dissolution of the silica decreased as the pretreating temperature increase; the degree of dissolution was different for various species. According to infrared absorption studies, the diatomaceous silica pretreated at a temperature below 500C preserved the reversibility of dehydration-rehydration almost qualitatively, while heating above 500C the samples lost the reversibility. Deformation of some kinks of diatomaceous silica pretreated above 500C, was percepted on infrared absorption spectra around 1,150 cm^–1. The samples pretreated up to 900 C, however, did not show such an internal structural change as could be identified by the X-ray diffraction analysis. The content of Al and Fe in diatomaceous silica was low and have not much difference in quality among diatom species. From these experimental results, it was adequately understandable that diatomaceous silica, which has a metastable character, is variable in the silica forms among species and its process and rate of transformation of internal structure and/or surface sites by the treatment at given temperatures also show differences.     

Organic geochemistry of the Japan Sea sediments-1: Bulk organic matter and hydrocarbon analyses of Core KH-79-3, C-3 from the Oki Ridge for paleoenvironment assessments

Organic geochemical study of bulk organic matter (OM), hopanoid hydrocarbon and normal hydrocarbon (C₂₃C₃₅) was conducted for a 936-cm-long sediment core sample from the Oki Ridge of the Japan Sea (Core KH-79-3, C-3; 37°03.5 N, 134°42.6E, water depth 935 m). Stable carbon isotopic ratios were also measured for both bulk OM and individual hydrocarbons. The following results were obtained: (1) The weight ratios of total organic carbon to total nitrogen range from 6.2 to 9.4 in the core. The ¹³C values of bulk OM range from –25.1–20.7%.. The ¹³C values of OM in the sections of 140190 cm are lower (–25–24) than those in the other sections (–23–21). This result indicates that OM in the core except for the 140190 cm sections is essentially of marine origin. (2) The ¹³C value of diploptene (a hopanoid hydrocarbon) in the last glacial maximum (LGM), is –66.3 (vs. PDB), which indicates it originating in methanotrophic bacteria. This result provides evidence to support for the previous ideas (Oba et al., 1980, 1984; Masuzawa and Kitano, 1984) that the bottom waters in the Japan Sea were anoxic in LGM. (3) Long chain (C₂₃C₃₅) n-alkanes of higher-plant wax origin were found throughout the core. Their concentration is high in 140190 cm in depth, suggesting that eolian dust load was high in LGM. (4) The n-alkane/TOC ratio increases with decreasing ¹³C values of bulk OM. This result indicates that the load of terrestrial (probably eolian dust-derived) OM to the Japan Sea became higher in colder climates. (5) The CPI values of long-chain n-alkanes are different in different ¹³O stages of paleoclimate, probably reflecting variations in species of terrestrial higher plants as a result of climatological adaptations.     

The distribution of geothermal fields along the East Pacific Rise from 13°10′ N to 8°20′ N: Implications for deep seated origins

In 1983 a combined SeaMARC I, Sea Beam swath mapping expedition traversed the East Pacific Rise from 13°20 N to 9°50 N, including most of the Clipperton Transform Fault at 10°15 N, and a chain of seamounts at 9°50 N which runs obliquely to both the ridge axis and transform fault trends. We collected temperature, salinity and magnetic data along the same track. These data, combined with Deep-Tow data and French hydrocasts, are used to construct a thermal section of the rise axis from 13°10 N to 8°20 N.Thermal data collected out to 25 km from the rise axis and along the Clipperton Transform Fault indicate that temperatures above the rise axis are uniformly warmer by 0.065°C than bottom water temperatures at equal depths off the axis. The rise axis thermal structure is punctuated by four distinct thermal fields with an average spacing of 155 km. All four of these fields are located on morphologic highs. Three fields are characterized by lenses of warmed water 20 km in length and 300 m thick. Additional clues to hydrothermal activity are provided in two cases by high concentrations of CH₄, dissolved Mn and ³He in the water column and in another case by concentrations of benthic animals commonly associated with hydrothermal regions.We use three methods to estimate large-scale heat loss. Heat flow estimates range from 1250 MW to 5600 MW for one thermal field 25 km in length. Total convective heat loss for the four major fields is estimated to lie between 2100 MW and 9450 MW. If we add the amount of heat it takes to warm the rest of the rise axis (489 km in length) by 0.065.°C, then the calculated axial heat loss is from 12,275 to 38,525 MW (19–61% of the total heat theoretically emitted from crust between 0 and 1 m.y. in age).     

Phase velocity of semi-diurnal internal waves at Ocean Weather Station T

Phase velocity of semi-diurnal internal waves is determined from differences between phases at three stations which were situated to form a triangle in the vicinity of sta. T (29N, 135E). The wave phases are estimated from temporal variations in depths of isotherms obtained from serial measurements of vertical temperature profiles at these stations. The measurements were carried out in cooperative operation of two vessels, the R. V.Tansei-maru and theNojima, during the period from 30 July to 1 August 1965. Wave propagation with the speed of about 2 m/s in the direction from east to west is obtained as an average over several isotherms of temperature from 19C to 23C. The area of measurement is to the west of Izu-Mariana ridge and the distance from the ridge to the station is about 500 km, which would be about 5 times as large as the wave length of the internal waves under consideration, and so it is possible to suppose that the internal waves observed generated at the ridge and propagated to the area without being subjected to serious refraction, scattering, reflection and decay.     

AOU as indicator of water-flow direction in the central North Pacific

Salinity, preformed phosphate and AOU distribution on the sigma-t surfaces of 26.8 and 27.3 of the central North Pacific Ocean, as well as the topography of these density surfaces, were studied. The direction of water flow suggested by the AOU distribution on these density surfaces was compared to that indicated by the acceleration potential contours on the _t =125 cl/ton and _t =80cl/ton surfaces drawn by Joseph L.Reid, Jr. The disagreements were explained in terms of mixing and possible gradients of primary production at the sea surface. On the 26.8 sigma-t surface a southward flow connecting the westward flow south of the Aleutian chain and the eastward flow farther south, between 175E and 180 is suggested by the AOU distribution but is not implied by the acceleration potential contours. If the circulation pattern at this density surface is similar to that at the sea surface, this southward flow is very likely to be real.     

The thermal effects of cooling system of a thermal power plant on photosynthesis of marine phytoplankton

The activity of photosynthesis and the contents of chlorophylla and pheo-pigments were compared in the samples of the intake and effluent seawater of Owase-Mita Thermal Power Plant and in the seawater samples in the Yoshimi Bay before and after heat treatment. At Owase-Mita Thermal Power Plant, after passage of sea water through the cooling system chlorophylla content reduced to about a half and pheo-pigment content doubled in August with high temperature (25–27C), but the contents of chlorophylla and pheo-pigments changed little in January with low temperature (16C); whereas the depression of photosynthetic activity after passing through the cooling system was 71–77 % in August and 31–46 % in January. In the laboratory experiments, the heat treatment at 34–43C for 7–8 sec., which resulted in temperature rise of 7–8C, gave the decrease of chlorophylla and an increase of pheo-pigments in August temperature (26.5C), but a slight decrease of chlorophylla and a slight increase of pheo-pigments in November at the initial temperature of 19.2C, while in January and December the samples with low initial temperature (11.6 and 10.8C) showed almost no change in the content of pigments; the decrease in photosynthetic activity was 32 % in August sample,ca. 11 % in November sample,ca. 15% in January and almost no change in December. It is concluded that the photosynthetic carbon assimilation of marine phytoplankton is suppressed by passing through the cooling system of a thermal power plant even at lower water temperature in winter, while the content of chlorophylla is affected very little at a temperature below 20C.     

Marine microbiological studies of mangrove swamps of Killai backwaters

The bacterial populations of mangrove swamps of Killai backwaters (11°21–11°29N, 79°46–79°50E, South India) were studied during August 1968 (Pre monsoon period) and December (post monsoon period). The presence of these groups such as agar digesters, algin digesters, cellulose digesters, sulphate reducers etc., bring about transformation of organic matter in the mangrove swamps. The presence of denitrifiers in mangrove swamps and in association with the molluscs may bring about the precipitation of calcium carbonate by removing the acid radicals such as sulphate and nitrite, increasing alkalinity. The luminiscent bacteria such asVibrio andAeromonas were also isolated in mangrove swamps of Killai backwaters. The iron bacteria likeLeptothrix sp. andGallionella sp. were also isolated from mangrove swamps of Killai backwaters.     

Variability of the axial morphology and of the gravity structure along the Central Spreading Ridge (North Fiji Basin): evidence for contrasting thermal regimes

The Central Spreading Ridge (CSR) is located in the central part of the North Fiji Basin, a complex back-arc basin created 12 Ma ago between the Pacific and Indo-Australian plates. The 3.5 Ma old CSR is the best developed, for both structure and magmatism, of all the spreading centers identified in the basin, and may be one of the largest spreading systems of the west Pacific back-arc basins. It is more than 800 km long and 50–60 km wide, and has been intensively explored during the French-Japanese STARMER project (1987–1991).The CSR is segmented into three first order segments named, from north to south, N160°, N15° and N-S according to their orientation. This segmentation pattern is similar to that found at mid-ocean ridges. The calculated spreading rate is intermediate and ranges from 83 mm/yr at 20°30 S to 50 mm/yr at 17°S. In addition, there is a change in the axial ridge morphology and gravity structure between the northern and southern sections of the CSR. The axial morphology changes from a deep rift valley (N160° segment), to a dome split by an axial graben (N15° segment) and to a rectangular flat top high (N-S segment). The Mantle Bouguer Anomalies obtained on the northern part of the CSR (N160°/N15° segments) show bull's eye structures associated with mantle upwelling at the 16°50S triple junction and also in the middle of the segments. The Mantle Bouguer Anomalies of the southern part of the ridge (N-S segment) are more homogeneous and consistent with the observed smooth topography associated with axial isostatic compensation.At these intermediate spreading rates the contrast in bathymetry and gravity structure between the segments may reflect differences in heat supply. We suggest that the N160° and N15° segments are cold with respect to the hot N-S segment. We use a non-steady-state thermal model to test this hypothesis. In this model, the accretion is simulated as a nearly steady-state seafloor spreading upon which are superimposed periodic thermal inputs. With the measured spreading rate of 50 mm/yr, a cooling cycle of 200,000 yr develops a thermal state that permits to explain the axial morphology and gravity structure observed on the N160° segment. A spreading rate of 83 mm/yr and a cooling cycle of 120,000 yr would generate the optimal thermal structure to explain the characteristics of the N-S segment. The boundaries between the hot N-S segment and its cold bounding segments are the 18°10 S and 20°30 S propagating rifts. A heat propagation event along the N-S segment at the expense of the adjacent colder failing segments, can explain the sharp changes in the observed morphology and structure between the segments.     

Sea Beam,SeaMARC II and ALVIN-based studies of faulting on the East Pacific Rise 9°20′ N–9°50′ N

A study of Sea Beam bathymetry and SeaMARC II side-scan sonar allows us to make quantitative measures of the contribution of faulting to the creation of abyssal hill topography on the East Pacific Rise (EPR) 9°15 N–9°50 N. We conclude that fault locations and throws can be confidently determined with just Sea Beam and SeaMARC II based on a number of in situ observations made from the ALVIN submersible. A compilation of 1026 fault scarp locations and scarp height measurements shows systematic variations both parallel and perpendicular to the ridge axis. Outward-facing fault scarps (facing away from the ridge axis), begin to develop within 2 km of the ridge and reach their final average height of 60 m at 5–7 km. Beyond these distances, outward-dipping faults appear to be locked, although there is some indication of continued lengthening of outward-facing fault scarps out to the edge of the survey area. Inward-facing fault scarps (facing toward the ridge axis), initiate 2 km off axis and increase in height and length out to the edge of our data at 30 km, where the average height of inward fault scarps is 60–70 m and the length is 30 km. Continued slip on inward faults at a greater distance off axis is probable, but based on fault lengths, 80% of the lengthening of inward fault scarps occurs within 30 km of the axis (>95% for outward faults). Along-strike propagation and linkage of these faults are common. Outward-dipping faults accommodate more apparent horizontal strain than inward ones within 10 km of the ridge. The net horizontal extension due to faulting at greater distances is estimated as 4.2–4.3%, and inward and outward faults contribute comparably. Both inward- and outward-facing fault scarps increase in height from north to south in our study area in the direction of decreasing inferred magma supply. Average fault spacing is 2 km for both inward-dipping and outward-dipping faults. The azimuths of fault scarps document the direction of ridge spreading, but they are sensitive to local changes in least compressive stress direction near discontinuities. Both the ridge trend and fault scarp azimuths show a clockwise change in trend of 3–5° from 9°50 N to 9°15 N approaching the 9° N overlapping spreading center.     

Magnetic studies in the northern Bay of Bengal

Total magnetic intensity and bathymetric surveys were carried out in the northern Bay of Bengal between 6° to 11° 45 N latitudes and east of 84° to 93° 30 E longitudes. The hitherto known 85° E Ridge is characterised as a subsurface feature by a large amplitude, positive magnetic anomaly surrounded by Mesozoic crust. A newly identified NE to NNESSW trending magnetic anomaly between 7° N, 87° 30 E and 10° 30 N, 89–90° E may be one of the unidentified Mesozoic lineations in the northern Bay of Bengal. The Ninetyeast Ridge is not associated with any recognizable magnetic anomaly. The Sunda Trough to the east of the Ninetyeast Ridge is characterised by a positive magnetic anomaly. A combined interpretation, using Werner deconvolution and analytical signal methods, yields basement depths ~ 10 km below sea level. These depths are in agreement with the seismic results of Curray (1991).Deceased 24 December 1991     

Effects of ligand speciation on determinations of the complexing abilities of strong ligands in natural waters

The presence of organic ligands (L) whose conditional stability constants (K_ML) are strong enough to allow them to form complexes with copper has been reported in oceanic waters. However, there is no general agreement on the distributional characteristics of such strong ligands in the water column. We present that these inconsistencies are derived from not only different analytical methods employed for their detection but also different oceanographic conditions. In particular, the nature and quantities of detectable natural ligands are affected by what kind of form the ligands are presentin situ in different marine environments, that is, chemical speciation of natural ligands (ligand speciation), which depends strongly on the variations in concentrations of coexisting trace metals. Using published data from observations in the laboratory and the field, we provide limits to the ranges of conditional stability constants of organic ligands for copper, zinc and cadmium that are detectable by the extensively used direct metal-titration method. For example, our model indicates that organic ligands for copper with log K_CuL(Cu)>12.4 in surface water and with log K_CuL(Cu)>9.9 in deep water might not have been detected because stronger ligands had formed complexes with ambient metalsin situ at a station in the North Pacific. The estimation suggests that there is a basinscale difference in speciation of natural organic ligands and, moreover, that this difference influences metal speciation. It is postulated that comparisons of the occurrence and levels of organic ligands might not be possible among spatially and temporally different observations without reconciliation of the effects of speciation of the ligands, even if an identical method is used in every case.     

Indirect evidence of deep flow east of a North Pacific seamount

East-west vertical sections centered on Kinmei Seamount (35N, 172E) of potential temperature, salinity, and oxygen, based on recent standard hydrographic measurements, and vertical profiles ofin situ temperature and density ( ₄) east of the seamount, based on STD data, show contrasting variability in the deep water characteristics east and west of the seamount. These data are consistent with horizontal variations in water mass properties (temperature-salinity and temperature-oxygen curves) east of the seamount between 2,600–3,900 m but not west of the seamount. East of the seamount on surfaces of constant potential temperature (or density) salinity and oxygen are higher at a station 200 km from the seamount than at stations on either side. From these relations and from known deep water properties of the North Pacific, it is suggested that east of the seamount, between 2,600–3,900 m, the observations are consistent with northward flow, which is interpreted to be a western boundary current with an east-west scale of about 300 km.