Echinoderms from Azores islands: An unexpected source of antibiotic resistant Enterococcus spp. and Escherichia coli isolates

The prevalence of antibiotic resistance and the implicated mechanisms of resistance were evaluated in Enterococcus spp. and Escherichia coli, isolated from a total of 250 faecal samples of echinoderms collected from Azorean waters (Portugal). A total of 144 enterococci (120 Enterococcus faecium, 14 E. hirae, 8 E. faecalis, 2 E. gallinarum) and 10 E. coli were recovered. High percentages of resistance in enterococci were found for erythromycin, ampicillin, tetracyclin and ciprofloxacin. The erm(A) or erm(B), tet(M) and/or tet(L), vat(D), aac(6′)-aph(2″) and aph(3′)-IIIa genes were found in isolates resistant to erythromycin, tetracycline, quinupristin/dalfopristin, high-level gentamicin and high-level kanamycin, respectively. Resistance in E. coli isolates was detected for streptomycin, amikacin, tetracycline and tobramycin. The aadA gene was found in streptomycin-resistant isolates and tet(A) + tet(B) genes in tetracycline-resistant isolates. The data recovered are essential to improve knowledge about the dissemination of resistant strains through marine ecosystems and the possible implications involved in transferring these resistances either to other animals or to humans.     

How charophytes (Streptophyta,Charales) survive in severe conditions of the permafrost area in Far North-East Asia

Eight species of charophytes have been found in the vast area of the ubiquitous permafrost in Far North-East Asia (i.e. the Republic of Sakha (Yakutia), the Magadan Region, and the Chukotka Autonomous Area, Russia): Chara contraria, C. globularis, C. strigosa, C. virgata, Nitella flexilis, N. opaca, N. wahlbergiana and Tolypella canadensis.Charophytes are found in a variety of water bodies and watercourses in areas with various thicknesses of permafrost, within the boreal and subarctic climate zones. Charophyte habitats are mostly associated with the distribution of carbonate rocks. The number of species and their records decrease towards north and east mainly due to shortening of the vegetation period. The highest density of records of charophytes and their most northern records are in river valleys, where the topography and heating effect of the river mitigate the influence of climate and permafrost. This association can be used to model and predict charophyte distribution in periglacial areas during glaciation.Widespread eurytopic species with a flexible life cycle, Chara contraria and C. globularis, are found in valley water bodies due to their ability to withstand environmental variation associated with marked changes in water temperature and flood disturbance. Rare perennial species such as C. strigosa and Tolypella canadensis are represented in deep lakes with less variation in environmental conditions.This paper discusses the distribution, habitat and life history strategies of charophytes that exist in the severe conditions of the permafrost area in Far North-East Asia based on previously published and new data.     

Chara can outcompete Myriophyllum under low phosphorus supply

In the course of re-oligotrophication in Lower Lake Constance, Germany, the tall-growing angiosperm Myriophyllum spicatum has been almost replaced by dense and lower-growing charophytes. We hypothesise that Chara globularis negatively affects the performance of M. spicatum due to density competition and nutrient interference. Intra- and interspecific competition was assessed using a response surface experimental design with different densities of both species in mono and mixed stands in an outdoor mesocosm experiment. After 8 weeks, we measured the growth and various functional traits of both species, including stoichiometry, ash-free dry mass and dry-matter content, and for M. spicatum, additionally chlorophyll content, leaf-mass fraction, formation of autofragments, and root/shoot ratio. With increasing density, C. globularis reduced the growth of M. spicatum much more strongly than that of conspecifics. Increasing density of C. globularis led to a lower chlorophyll a to b ratio and lower nitrogen content based on ash-free dry mass in M. spicatum as well as reduced autofragmentation. Established C. globularis meadows can negatively affect tall-growing angiosperms such as M. spicatum when the environmental conditions, such as low phosphorus availability and high water-clarity, are appropriate. These findings have implications for the management of lakes, specifically those where a nuisance growth of tall macrophytes occurs, e.g. in systems where M. spicatum is invasive.     

Implications of turbulent shear on clay floc break-up along the Atlantic estuary (Bouregreg), Morocco

Water depth,salinity,current,and suspended sediment concentration(SSC)were measured along with the grain size distribution of bed sediment along an estuarine longitudinal section.The floc size increased with increase in the percentage of clay and silt,while decreased with increase in the percentage of sand content of bed sediment.The turbulent shear,G,had a direct effect on floc size with its value increasing with increase in G up to a G value of 15 s^-1,while an inverse relation existed between floc size and G at higher G(G>15 s^-1).Further,higher turbulence enabled sand to get resuspended and cause additional shear leading to the break-up of flocs.An attempt was made to modify G to account for the combined effect of water turbulence(G)and shear imparted by sand(Ga)and the impact of the modification of G on the predictability of floc size was evaluated.A new model was developed which explains floc size in terms of sediment concentration(C),salinity gradient(S),and G for different scenarios based on the value of G.Sensitivity analysis was done for observed floc size(FS)and predicted floc size using four approaches:(I)FSαC^x;(II)FSαC^xS-y;(III)FSαC^xS^-yGz for G<15 s^-1and FSαC^xS^-yG-z for G>15 s^-1;and(IV)FSαC^xS^-yG_m^-zfor G>15 s^-1and G_m=G+Ga,where x,y,and z are determined by calibration.It was observed that the predictability of the floc size improved when the turbulence was modified to account for shear imparted by sand so that the coefficient of determination was increased from 0.78 for model III to 0.89 for model IV.Further,the settling velocity was expressed as a function of suspended sediment concentration,turbulent shear,and salinity gradient.The predictability of settling velocity was improved(R2 increased from 0.77 to 0.86)when the additional turbulence created by sand was incorporated in the non-dimensional empirical equation.The study highlights the influence of sand in causing the break-up of flocs and suggests that for turbulence shear values high enough to resuspend sand,and G has to be modified to account for the additional shear imparted by sand in mixed sediment estuarine environments.     

On the physical essence of theb value for AE of rock tests and natural earthquakes in terms of fracture mechanics

The problem of physical essence of theb value forAE of rock tests and natural earthquakes has been a controversial topic during the past two decades. In the present paper the order and energy of each microfracturing of the microcrack system existing in a rock specimen is discussed from the viewpoint of fracture mechanics, then the whole series ofAE and subsequently the value ofb are determined. The order of microfracturing depends on the parametersKei/Kci whereKei is the effective stress intensity factor of thei-th microcrack andKci is the fracture toughness in the site of thei-th crack. The energy of eachAE can be expressed asηi ∫ loili Gids whereG is the energy release rate of microcarck,loi andli are the original and final crack lengths respectively andηi is the emanating efficiency for thei-th crack. If we assume that the distribution density function of microcrack lengthl isp(l)=Bl?v whereB andγ are constants, then the expressionb=3γ/2 can be deduced. Hence, we have come to the conclusion that the value ofb mirrors essentially the “crack-system-configuration” of the material, which means the distribution of the sizes, shapes and orientations of microcracks over space as well as the distribution of the other relevant physical parameters such as fracture toughness, friction coefficient, etc. Our conclusion is somewhat similar in character to Mogi’s. He concluded thet the heterogeneity of the material plays the most important role in determining the value ofb. The term heterogeneity of course covers the idea of “crack-system-configuration, but we think that our view has a little bit deeper insight to the problem than that of Mogi.     

Information,artifacts, and noise in dQ/dt − Q recession analysis

The plotting of the time rate of change in discharge dQ/dt versus discharge Q has become a widely used tool for analyzing recession data since Brutseart and Nieber [Water Resour Res 13 (1977) 637–643] proposed the method. Typically the time increment Δt over which the recession slope dQ/dt is approximated is held constant. It is shown here this that leads to upper and lower envelopes in graphs of log(−dQ/dt) versus log(Q) that have been observed in previous studies but are artifacts. The use of constant time increments also limits accurate representation of the recession relationship to the portion of the hydrograph for which the chosen time increment is appropriate. Where dQ/dt varies by orders of magnitude during recession, this may exclude much of the hydrograph from analysis. In response, a new method is proposed in which Δt for each observation in time is properly scaled to the observed drop in discharge ΔQ. It is shown, with examples, how the new method can succeed in exposing the underlying relationship between dQ/dt and Q where the standard method fails.     

Space–Time Earthquake Prediction: The Error Diagrams

The quality of earthquake prediction is usually characterized by a two-dimensional diagram n versus τ, where n is the rate of failures-to-predict and τ is a characteristic of space–time alarm. Unlike the time prediction case, the quantity τ is not defined uniquely. We start from the case in which τ is a vector with components related to the local alarm times and find a simple structure of the space–time diagram in terms of local time diagrams. This key result is used to analyze the usual 2-d error sets {n, τ _w } in which τ _w is a weighted mean of the τ components and w is the weight vector. We suggest a simple algorithm to find the (n, τ _w ) representation of all random guess strategies, the set D, and prove that there exists the unique case of w when D degenerates to the diagonal n + τ _w  = 1. We find also a confidence zone of D on the (n, τ _w ) plane when the local target rates are known roughly. These facts are important for correct interpretation of (n, τ _w ) diagrams when we discuss the prediction capability of the data or prediction methods.     

A stability analysis of a conduit flow model for lava dome eruptions

Periodic variations in magma discharge rate and ground deformation have been commonly observed during lava dome eruptions. We performed a stability analysis of a conduit flow model by Barmin et al. [Barmin, A., Melnik, O., Sparks, R.S.J., 2002. Periodic behavior in lava dome eruptions. Earth and Planetary Science Letters 199 (1-2), 173–184], in which the periodic variations in magma flow rate and chamber pressure are reproduced as a result of the temporal and spatial changes of the magma viscosity controlled by the kinetics of crystallization. The model is reduced to a dynamical system where the time derivatives of the magma flow rate (dQ/dt) and the chamber pressure (dP/dt) are functions of Q and P evaluated at a shifted time t − t_?. Here, the time delay t_? represents the time for the viscosity of fluid particle to increase in a conduit. The dynamical system with time delay is approximated by a simple two-dimensional dynamical system of Q and P where t_? is given as a parameter. The results of our linear stability analyses for these dynamical systems indicate that the transition from steady to periodic flow depends on nonlinearities in the steady state relation between Q and P. The steady state relation shows a sigmoidal curve in Q − P phase plane; its slope has negative values at intermediate flow rates. The steady state solutions become unstable, and hence P and Q oscillate periodically, when the negative slope of the steady state relation ([dP/dQ]_S) exceeds a critical value; that is [dP/dQ]_S < − t_?γ/(2V_ch), where V_ch is the chamber volume and γ is an elastic constant which is related to the rigidity of chamber wall. We also found that the period and the pattern of oscillation of the conduit flow primarily depend on a quantity defined by LV_ch/r⁴, where L is the conduit length and r is the conduit radius.     

Spatial and Temporal Variation of LURR and its Implication for the Tendency of Earthquake Occurrence in Southern California

— Based on the theory of LURR and its recent development, spatial and temporal variation of Y/Y _c (value of LURR/critical value of LURR) in the Southern California region during the period from 1980 through March, 2001 was studied. According to the previous study on the fault system and stress field in Southern California, we zoned the Southern California region into 11 parts in each of which the stress field is almost uniform. With the time window of one year, time moving step of three months, space window of a circle region with a radius of 100 km and space moving step of 0.25 degree in latitude and longitude direction, the evolution of Y/Y _c were snapshot. The scanning results show that obvious Y/Y _c anomalies occurred before 5/6 of strong earthquakes considered with a magnitude of 6.5 or greater. The critical regions of Y/Y _c are near the epicenters of the strong earthquakes and the Y/Y _c anomalies occur months to years prior to the earthquakes. The tendency of earthquake occurrence in the California region is briefly discussed on the basis of the examination of Y/Y _c .     

Amino acid racemization in four species of ostracodes: Taxonomic,environmental, and microstructural controls

Here we quantified the aspartic acid and glutamic acid racemization rates of the four main ostracode species (Herpetocypris reptans, Candona neglecta, Ilyocypris gibba and Cyprideis torosa) present in several Iberian Peninsula localities covering a wide chronological range (ca. 1 Ma to present). At low D/L values (at Asp D/L < 0.40; and Glu D/L = 0.09–0.18), H. reptans racemized at higher rates than C. neglecta, C. torosa and I. gibba. In contrast, for Asp D/L > 0.4 and Glu D/L > 0.18, H. reptans, C. neglecta and C. torosa showed similar racemization rates. I. gibba exhibited the lowest D/L values in old samples (Middle and Lower Pleistocene). We attribute these differences in amino acid racemization rates mainly to variations in valve protein composition. We found that the microstructure of the valves of each species (size, morphology, and arrangement of crystals) differed, but did not appear to change over time (at least for the last ca. 1 Ma). Such differences may also be linked to the type of proteins involved in the respective calcification processes of these organisms. On the basis of our results, and given that other studies have demonstrated that the majority of inter-crystalline proteins are leached early after death (a few centuries or millennia), we propose that the degradation rates of the most resistant inter- and intra-crystalline proteins in each species differ depending on the protein composition of the valves. Although further research is required, we suggest that amino acid racemization in each ostracode species might be related to valve microstructure.     

Assessment of heavy metals/metalloid (As,Pb, Cd,Ni, Zn,Cr, Cu,Mn) concentrations in edible fish species tissue in the Pearl River Delta (PRD), China

The major aim of this study was to investigate heavy metal content of edible fish in the PRD. Eleven species of fish (consisting of 711 individuals) [catfish (Clarias fuscus), tilapia (Oreochromis mossambicus), grass carp (Ctenopharyngodon idellus), bighead carp (Aristichthys nobilis), mandarin fish (Siniperca kneri), snakehead (Channa asiatiea), black bass (Micropterus salmoides), mangrove snapper (Lutjanus griseus), star snapper (Lutjanu stellatus), snubnose pompano (Trachinotus blochii) and orange-spotted grouper (Epinephelus coioides)] were collected for the analyses of heavy metals. Overall concentrations (mg/kg, ww) in the fish muscles were: As (0.03–1.53), Pb (0.03–8.62), Cd (0.02–0.06), Ni (0.44–9.75), Zn (15.7–29.5), Cr (0.22–0.65), Cu (0.79–2.26), Mn (0.82–6.91). Significant level of Pb were found in tilapia at all locations. It is recommended that heavy metal concentrations in different fish species must be determined on a regular basis in the future so as to reduce human health risks from acute and chronic food intoxication.     

Scattering and intrinsic attenuation in Cairo metropolitan area using genetic algorithm

A total number of 46 local earthquakes (2.0≤ML≤4.0) recorded in the period 2000–2011 by the Egyptian seismographic network (ENSN) were used to estimate the total (Q_t⁻¹), intrinsic (Q_i⁻¹) and scattering attenuation (Q_sc⁻¹) in Cairo metropolitan area, Egypt. The multiple lapse time window analysis (MLTWA) under the assumption of multiple isotropic scattering with uniform distribution of scatters was firstly applied to estimate the pair of L_e⁻¹, the extinction length inverse, and B₀, the seismic albedo, in the frequency range 3–24 Hz. To take into account the effect of a depth-dependent earth model, the obtained values of B₀ and L_e⁻¹ were corrected for an earth structure characterized by a transparent upper mantle and a heterogeneous crust. The estimated values of Q_t⁻¹, Q_sc⁻¹ and Q_i⁻¹ exhibited frequency dependences. The average frequency-dependent relationships of attenuation characteristics estimated for the region are found to be: Q_t⁻¹=(0.015±0.008)f ^{(−1.02±0.02)}, Q_sc⁻¹=(0.006±0.001)f ^{(−1.01±0.02)}, and Q_i⁻¹=(0.009±0.008)f ^{(−1.03±0.02)}; showing a predominance of intrinsic absorption over scattering attenuation. This finding implies that the pore-fluid contents may have great effect on the attenuation mechanism in the upper crust where the River Nile is passing through the study area. The obtained results are comparable with those obtained in other tectonic regions.     

Biological accumulation of chlordane compounds in marine organisms from the northern North Pacific and Bering Sea

Sum of chlordane compounds (ΣCHL; cis-chlordane + trans-chlordane + cis-nonachlor + trans-nonachlor + oxychlordane) are concentrated gradually with trophic levels from zooplankton to Dall's porpoise (Phocoenoides dalli) through squid and fish. The order of bioconcentration factors (BCF: concentration in organism/concentration in seawater) in these organisms was ΣDDT (p,p′-DDE + p,p′-DDD + p,p′-DDT)>ΣCHL≦PCBs>ΣHCH (α-HCH + β-HCH + γ-HCH). Calculation for the concentration factor against food, namely biomagnification factor (BMF: concentration in organism/concentration in its food), was made for Dall's porpoise and thick-billed murre (Uria lomvia). The BMFs of these chemicals in thick-billed murre were lower than those of Dall's porpoise, suggesting the degradation and/or excretion of organochlorines through the uropygial gland with lipids. Moreover, the lowest BMF of ΣCHL in thick-billed murre among organochlorines may indicate that chlordane compounds (CHLs) are metabolized more rapidly by this seabird than Dall's porpoise.     

Three-Dimensional V P and V P /V S Structure in the Longtan Reservoir Area by Local Earthquake Tomography

High-resolution three-dimensional V _P and V _P /V _S images in the Longtan reservoir area were obtained from local earthquake data by using 3,178 events with total 24,153 P-wave and 23,987 S-wave arrivals collected from 23 seismic stations. The tomographic images show that significant V _P heterogeneities can be seen at layers of different depth in the Longtan reservoir area. Low-V _P anomalies both beneath and around the main rivers in the reservoir area may be related to the composition of rocks which are mainly deposit carbonate and arenaceous shale, which contributes to water saturation. We deduced that the high porosity rocks beneath the main rivers may be fully saturated with water. The phenomenon that V _P is relatively high in the area which is 10–20?km away from the rivers indicates that horizontal saturation of water is limited within a small range of area that is about 10–20?km from the main rivers. The characteristic is significant that seismicity in the Longtan reservoir area is coincident with the distribution of the low-V _P area. V _P /V _S tomographic images show that V _P /V _S ranges from 1.8 to 2.05 in shallow layers above 4?km depth beneath the Longtan reservoir, suggesting the properties of the rocks are limestone and shale. At the depth of 7?km, the distribution of V _P /V _S image varies quite remarkably, especially in the dam area. This demonstrates that the range of influence by the saturation of water in the media below the reservoir surface can reach 4–7?km depth in the dam area.     

Equivalent diffusion coefficient of clay-rich geological formations: comparison between numerical and analytical estimates

Laboratory experiments in rock samples collected from clay-rich formations indicate that the effective molecular diffusion coefficient (D) is a heterogeneous and anisotropic property. Since laboratory measurements of D are representative of a very small volume, upscaling is necessary in order to incorporate these data in large-scale numerical models of diffusive transport. In this work we address the problem of the estimating the equivalent diffusion coefficient (D _eq ), in terms of total diffusive flux, in a three-dimensional domain characterized by a heterogeneous and anisotropic spatial distribution of D. D _eq was estimated from the results of steady-state diffusive transport simulations through several realizations of the D field. The ensemble averages of D _eq from fields with different degrees of heterogeneity and anisotropy were then compared with estimates from analytical upscaling expressions based on stochastic as well as power-averaging approaches. These expressions are largely based on similar expressions developed for calculating the effective hydraulic conductivity in heterogeneous and anisotropic domains. Comparisons showed that stochastic expressions provide accurate estimates of D _eq only for fields characterized by low heterogeneity. Within the range of heterogeneity and anisotropy considered, our results showed that a power-averaging expression is very accurate in predicting D _eq especially when the parameter p _i is estimated through fitting of the numerical results. Nonetheless, the relationship between this parameter and the anisotropy ratio is linear.     

Effects of the modified Huanglian Jiedu decoction on the disease resistance in grey mullet (Mugil cephalus) to Lactococcus garvieae

Lactococcosis is prevalent on grey mullet (Mugil cephalus) in Hong Kong aquaculture resulting in serious economic loss. A compound formulation of Traditional Chinese Medicines (TCM) (modified Huanglian Jiedu decoction (HLJDD)) comprising Rhizoma coptidis, Radix scutellaria, Cortex phellodendri, Fructus gardeniae, Fructus forsythiae and Flos lonicerae japonicae (in a ratio of 3:2:2:3:3:5) were applied as feed supplements to deal with the disease. The Nitroblue tetrazolium activity in blood, bactericidal activity and total immunoglobulin in plasma were significantly enhanced after feeding 1% of this TCM for 28 days. The disease resistances to Lactococcus garvieae in 1% and 2% TCM feeding groups were significantly enhanced. In the in vitro study, the modified HLJDD also activated the plasma bactericidal activities (p < 0.01). Based on this study, 1% modified HLJDD feeding for 28 days may be an optimal dose to prevent L. garvieae infection and could be used in aquaculture industries.     

Temporal variations of seismicity parameters in the Central Alborz,Iran

Temporal changes of b-value, fractal (correlation) dimensions of epicenters (D _e ) and occurrence time of earthquakes (D _t ) and relations between these parameters were calculated to investigate precursory changes before 28 May 2004, Baladeh-Kojour earthquake (M _w = 6.3) of Central Alborz, Iran. 2086 events with M _N ≥ 1.7 were selected for our analyses. A wide range of variation was seen in these parameters: b-value ～ 0.6–1.11, D _e ～ 0.97–1.64, and D _t ～ 0.13–0.93. The results showed decreases in all fractal parameters several months before the main shock. This decrease, which might have arisen due to clusters of events occurred between 2002–2003, was followed by a systematic increase, corresponding to the increased level of low-magnitude seismicity. It seems that changes in fractal parameters may be precursors of Baladeh-Kojour earthquake which was caused by seismic activation and quiescence. Furthermore, a positive correlation between b-value and D _e was detected before the main shock (D _e = 0.87 + 0.7b) and during aftershock sequences (D _e = 2b ± 0.09), which was further on changed to a negative one (D _e = 2.56–1.32b).     

Frequency-dependent Attenuation of High-frequency P and S Waves in the Upper Crust in Western Nagano, Japan

—Borehole seismograms from local earthquakes in the aftershock region of the 1984 western Nagano Prefecture, Japan earthquake were analyzed to measure the frequency-dependent characteristics of P- and S-wave attenuation in the upper crust. The records from a three-component velocity seismometer at the depth of 145m exhibit high S/N-ratio in a wide frequency range up to 100 Hz. Extended coda normalization methods were applied to bandpass-filtered seismograms of frequencies from 25 to 102 Hz. For the attenuation of high-frequency P and S waves, our measurements show Q _P ^-1? 0.052?^-0.66 and Q _S ^-1? 0.0034?^-0.12 respectively. The frequency dependence of the quality factor of S waves is very weak as compared with that of P waves. The ratio of Q _P ^-1/Q _S ^-1 is larger than unity in the entire analyzed frequency range.     

Molecular characterizations of chloramphenicol- and oxytetracycline-resistant bacteria and resistance genes in mariculture waters of China

In order to gain an understanding of the diversity and distribution of antimicrobial-resistant bacteria and their resistance genes in maricultural environments, multidrug-resistant bacteria were screened for the rearing waters from a mariculture farm of China. Both abalone Haliotis discushannai and turbot Scophthalmus maximus rearing waters were populated with abundant chloramphenicol-resistant bacteria. These bacteria were also multidrug resistant, with Vibriosplendidus and Vibriotasmaniensis being the most predominant species. The chloramphenicol-resistance gene cat II, cat IV or floR could be detected in most of the multidrug-resistant isolates, and the oxytetracycline-resistance gene tet(B), tet(D), tet(E) or tet(M) could also be detected for most of the isolates. Coexistence of chloramphenicol- and oxytetracycline-resistance genes partially explains the molecular mechanism of multidrug resistance in the studied maricultural environments. Comparative studies with different antimicrobial agents as the starting isolation reagents may help detect a wider diversity of the antimicrobial-resistant bacteria and their resistance genes.