锈色尖毛虫(Oxytricha ferruginea)的形态学重描述及与相近种的比较(纤毛门,腹毛目)

利用活体观察、蛋白银染色技术对淡水腹毛目纤毛虫—锈色尖毛虫 (Oxytricha ferrug-inea Stein,185 9)的青岛种群之活体形态学及纤毛图式做了研究。其特征如下 :虫体浅黄褐色 ;表膜下颗粒为铁锈色 ,聚合成组并作纵向不规则排列。口围带平均小膜数为 35。额、腹、横棘毛为典型8∶ 5∶ 5方式排布 ;尾棘毛恒为 1根。背触毛 5列 ,其中 4列完整 ,1列不完整。伸缩泡位于体左侧前1/ 3处 ,具两根收集管。与相近种比较表明 ,O.ferruginea与前人所报道的另一淡水种 O.aerugi-nosa极可能是同一种 ,因此本文建议将两者合并。     

游仆虫科下阶元系统关系的探讨(纤毛门,腹毛目)

借助聚类分析和近祖演化推测（支序分类方法）对纤毛门原生运行中一高级类群－游仆虫科内各属间系统关系及科内地位予以分析探讨。结果建议：所涉各成员可细分为７个亚群，按演化顺序依次为Ｇａｓｔｒｏｃｉｒｒｈｕｓ；Ｓｗｅｄｍａｒｋｉａ－Ｄｉｓｃｏｃｅｐｈａｌｕｓ；Ｕｒｏｎｙｃｈｉａ；Ｄｉｏｐｈｒｙｓ；Ｅｕｐｌｏｔｉｄｉｕｍ－Ｃｅｒｔｅｓｉａ；Ｃｙｔｈａｒｏｉｄｅｓ－Ｅｕｐｌｏｔｅｓ；Ｅｕｐｌｏｔａｓｐｉｓ－Ａ     

九脊游仆虫(Euplotes novemcarinata Wang,1930)的纤毛下器与银线系研究(纤毛动物门,腹毛目)

九脊游仆虫(Euplotes novemcarinata Wang、1930)是土壤及淡水中生活的原生动物。本文对其重要的分类特征诸如银线系及纤毛下器做了形态学研究,确认了其分类地位,并比较了国外迄今的研究工作,在此基础上,将同物异名苔藓游仆虫(E.muscicolaKahl、1932)作了归并。     

南极海胆体内共栖纤毛虫三新种及一新属(原生动物,纤毛门)(英文)

1992年4～5月于南极冰层下动物区系考察中（Polarstern号，航次ANT，×／3）对内栖于海胆消化道内的3种纤毛虫新种做了形态学的研究。结果表明此三种分别隶属于寡膜纲和多膜纲内的三个属，包括一新属──拟内盘虫属。该新属特征为：体左右高度测扁，具拟尾丝虫状的口器结构及腹侧“缩短”的体动基列。新种南极裂核虫与其同属内已知种相比具有数目更多、密集排列的体动基列，右置的胞肛及不同的虫体外形。另一新种海胆旋游虫则具有十分独特的体纤毛图式：开放式的赤道动基列在尾端分成两部分（GK，PK1）。     

车轮虫属(原生动物,纤毛门)种类鉴定的方法学

车轮虫属的种间区分长期以来一直是生产实践中该类病原鉴定的突出难点之一。结合作者及前人的研究工作 ,该文提出一适用于车轮虫属内种类鉴定的综合方法与简明要旨。文中对车轮虫的形态学 ,包括活体外形、齿体、辐线、附着盘中央颗粒、核器及口围绕体等进行了简要地叙述及图解式介绍 ;对车轮虫附着盘中央颗粒的分类学意义进行了总结和评析 ;同时 ,对车轮虫的生境特征在种类鉴定上的意义做了探讨。     

中国海域南褐虾属种类记述(甲壳动物亚门, 十足目, 褐虾科)

为了搞清中国海域存在的南褐虾(Philocheras)的生物多样性和地理分布,作者通过形态学特征系统研究了中国科学院海洋研究所海洋生物标本馆馆藏的南褐虾标本,此标本主要采自中国海域,也检查了部分采自菲律宾海域的标本。本研究和相关文献的结果表明:中国海域存在的南褐虾共计7种,包括作者描绘的4种以及在其他文献中提到的3种。作者对本次研究的4种南褐虾提供了详细的鉴别特征、地理分布、外部整体图和附肢结构图,并对中国海域存在的7种南褐虾提供了检索表。     

共栖于对虾体表的致病性纤毛虫Ⅱ.(纤毛动物门,缘毛目)

作为系列报道的第二部分记述了黄渤海区共栖于对虾体表的3属15种缘毛类纤毛虫,其中包括8种国内新记录及聚缩虫属的一新种——拟钩虾聚缩虫。文中对所涉种类的形态学特征做了概括性描述及图示,以期为对虾养殖中病原的调查鉴定及进一步的动物区系研究提供一基础资料。     

中国海域海盘车科(棘皮动物门,海星纲)种类记述

基于中国科学院海洋生物标本馆的馆藏标本,通过传统形态学,结合扫描电子显微镜等方法,对分布于中国海域的海盘车科(Asteriidae)种类进行了系统的分类学研究,共鉴定出该科5属10种,分别为:粗钝海盘车(Asterias argonauta Djakonov, 1950)、多棘海盘车(Asterias amurensis Lütken, 1871)、罗氏海盘车(Asterias rollestoni Bell, 1881)、异色海盘车(Asterias versicolor Sladen, 1889)、日本滑海盘车(Aphelasterias japonica (Bell, 1881))、张氏滑海盘车(Aphelasterias changfengyingi Baranova Wu, 1962)、日本长腕海盘车(Distolasterias nipon(D?derlein,1902))、美丽长腕海盘车(Distolasterias elegans Djakonov,1931)、座冠海星[Coronaster volsellatus(Sladen,1889)]和尖棘筛海盘车[Coscinasterias acutispina(Stimpson,1862)]。文中对物种的鉴别特征进行了描述,并提供了地理分布和种属鉴定所需要的检索表。     

共栖于对虾体表的致病性纤毛虫Ⅰ.(纤毛动物门,缘毛目)

采用现代通行的蛋白银染色法及Foissner干银法对共栖在对虾体表的钟虫属11种致病性纤毛虫做了形态分类学研究。报道了5种国内新记录。对所涉种类的银线系及纤毛下器做了首次揭示与描述,并列表对诸种的分类特征进行了统计学界定。     

On four closely related hypotrichous ciliates (Protozoa, Ciliophora, Spirotrichea): molecular characters, interspecific relationships and phylogeny defined with multigene sequence information

In order to clarify the phylogeny and relationships of the most confused hypotrichous ciliates, Holosticha-complex, four closely related holostichids (five populations), Holosticha bradburyae, H. diademata, Anteholosticha sp., and A. manca, were compared and analyzed using ITS2 secondary structures, ITS1-5.8S-ITS2 region and SSrRNA gene sequences. The ITS1-5.8S-ITS2 region sequences of these four species were first sequenced, and they shared sequence identities ranging from 68.0% to 90.1%, while two populations of Anteholosticha sp. differed in three nucleotides (sequence identity 99.8%). There were several minor differences among ITS2 secondary structures of these species, while two populations of Anteholosticha sp. had the identical secondary structure. Phylogenetic trees inferred from the ITS1-5.8S-ITS2 region sequences of stichotrichs using multiple algorithms (Neighbor-Joining, Maximum Parsimony and Bayesian) revealed similar topologies. The results show that:(1) Holosticha bradburyae and H. diademata firmly clustered together with strong bootstrap supports, forming a sister clade with Anteholosticha sp., (2) Anteholosticha appeared to be a paraphyletic assemblage, in which the morphotype A. manca was more closely related to Diaxonella trimarginata than to its congener Anteholosticha sp. Phylogenetic analyses based on the SSrRNA gene and the combined sequences of SSrRNA gene and ITS1-5.8S-ITS2 region revealed the similar relationships between Holosticha and Anteholosticha, nevertheless their positions within the subclass Stichotrichia differed from each other inferred from different genes.     

五种管口类纤毛虫的形态学研究

管口目纤毛虫由于个体微小且大部分种类缺乏现代分类学的研究,是纤毛门中较混乱的类群之一。为进一步了解其形态性状的种群间差异,借助显微活体观察及蛋白银染色法对采自青岛、湛江和香港近岸的斯雷腹沟虫(Coeloperix sleighi)、尖绒毛虫(Microxysma acutum)、乙状轮毛虫(Trochilia sigmoides)、阿氏直毛虫(Orthotrochilia agamalievi)和近岸类偏体虫(Agnathodysteria littoralis)5种管口类纤毛虫进行了形态学再研究,依据新采集种群,对其活体形态和纤毛图式特征进行补足性描述。结果发现,斯雷腹沟虫、乙状轮毛虫和阿氏直毛虫的形态性状极为稳定,而新采集的尖绒毛虫和近岸类偏体虫种群在伸缩泡数目上同前人描述略有差异。     

Ibla cumingi (Crustacea, Cirripedia) -A Gonochoristic Species (Anatomy, Dwarfing and Systematic Implications)

Abstract. Ibla cumingi (Crustacea, Cirripedia) is a gonochoristic species with dwarf males. The anatomy of both sexes is described and compared with that of other Cirripedia s. str. Systematic implications on the basis of anatomy and biology are given. The degree of dwarfing in the male and the possible cause and way of reduction are discussed.     

Separation and relationship of ten marine scuticociliates (Protozoa, Ciliophora) using RAPD fingerprinting method

On the basis of the random amplified polymorphic DNA-fingerprinting (RAPD) method, 10 morphospecies of scuticociliates from 7 genera, including 15 clones of 13 strains, Uronema marinum, Uronema cf marinum, Parauronema virginianum, P. longum, Metanophrys similis, M. sinensis, Paralembus digitiformis, Mesanophrys carcini, Paranophrys magna and Cohnilernbus verrninus were analysed using 8 oligonucleotide primers. The genetic similarity among the clones of the same strain measured by a band-sharing index is 0.97～0.98,while 0.40-4).52 among strains. This value measured is 0.39～0.46 among congeners of the same genus, whereas 0.16～0.47 between different genera. A distance tree was constructed based on 8-primer analysis, in which the scuticociliates investigated were separated into two clusters: one consists of 2 genera, Uronema and Parauronema, and the other was composed of five, Metanophrys, Mestmophrys,Paranophrys, Paralembus and Cohnilembus. It is demonstrated also that the morphospecies Parauronema virginianurn may be a species-complex, i. e., it contains different genospecies.     

Morphogenetic studies on Uronychia uncinata(Protozoa, Ciliophora) during its asexual division

ＭｏｒｐｈｏｇｅｎｅｔｉｃｓｔｕｄｉｅｓｏｎＵｒｏｎｙｃｈｉａｕｎｃｉｎａｔａ（Ｐｒｏｔｏｚｏａ，Ｃｉｌｉｏｐｈｏｒａ）ｄｕｒｉｎｇｉｔｓａｓｅｘｕａｌｄｉｖｉｓｉｏｎ￥ＳｏｎｇＷｅｉｂｏ（ＲｅｃｅｉｖｅｄＡｕｇｕｓｔ２８，１９９５；ａｃｃｅｐｔｅｄ...     

海水养殖鱼类外寄生车轮虫II.拟车轮虫属、小车轮虫属及两分虫属

依据干银法和蛋白银法染色法研究了黄渤海沿岸经济鱼类寄生的车轮虫科内 3属 4种纤毛虫 :球核拟车轮虫、斜拟车轮虫、劳牧小车轮虫及简单两分虫。其中球核拟车轮虫和斜拟车轮虫为我国的新记录。文中对所涉车轮虫的形态学、宿主、分布、流行情况等做了报道。     

渤海1999年4月运动类铃虫的平面分布

研究了1999年4月末用浅海Ⅲ型网在渤海30个站住采集的浮游生物样品中的砂壳纤毛虫,发现砂壳纤毛虫种类单一,几乎全部为运动类铃虫(Codonellopsis mobilis Wang 1936),丰度为32～10731个／m^3,碳生物量为0～1．52mg／m^3。     

海水养殖水体中危害性纤毛虫原生动物研究Ⅰ.盾纤目习见类群的口器结构及鉴别特征

对发现自我国北方海区养殖环境中危害性盾纤目纤毛虫的常见类群 (16属 )之口器结构、一般形态学与鉴别特征以及属种模式做了概要性介绍。同时涉及了生活类型、已知种的分布范围、纤毛图式水平的示差鉴别及属级定义并借助模式图例为该类广泛分布而又不易识别的病害纤毛虫类群提供了一份简明的病原鉴定资料