斑节对虾高密度养殖配合饲料的研制

为了适应斑节对虾高密度养殖业迅猛发展的需要,研制了粤海牌斑节对虾饲料。该饲料配方合理,工艺先进,不加粘结剂,能达到水中稳定性的要求。试验结果表明。饵料系数１．３９－２．１８,饲养７９－１２６ｄ,产量６８７０－９２４０ｋｇ／ｈｍ＾２,收规格５４－７２属／ｋｇ,饲养的斑节对虾肌肉饱满,活力强。     

不同形式钴对凡纳滨对虾生长和组织钴含量的影响

为研究氨基酸鳌合钴对凡纳滨对虾的营养效果．在凡纳滨对虾配合饲料中添加5、10、15mg／kg的氯化钴和蛋氨酸钴。饲养8周．4周末（饲养中期）称重．8周末称重并采集对虾肝胰脏和肌肉．测定肝胰脏和肌肉中的钴含量。结果表明：钴的添加形式和钴水平不影响对虾0～4周增重率．15mg／kg蛋氨酸钴显著提高了对虾0～8周增重率，且两种形式的钴之间差异显著（P〈0．05）。5、10mg／kg的蛋氨酸钴和5、10、15mg／kg的氯化钴不影响8周末对虾的增重率。特定生长率、饲料系数和蛋白质效率的变化趋势与增重率类似。钴的添加形式和钴水平不影响肌肉中的钴含量。15mg／kg的氯化钴组肝胰脏中钴含量显著高于对照组（P〈0．05）．其他各组间无显著差异；蛋氨酸钴各组肝胰脏中钴含量无显著差异；钴的添加形式不影响肝胰脏中钴含量。统计结果表明。促进生长方面，两种钴之间存在显著差异，蛋氨酸钴的营养效果好于氯化钴。饲料中蛋氨酸钴的添加量为15mg／kg时．对虾生长最快．5mg／kg时．可满足需要。     

4种对虾耗氧率、CO_2排出量、呼吸商和窒息点的研究（英文）

较系统研究了斑节对虾、墨吉对虾、日本对虾和刀额新对虾等４种对虾的耗氧率、ＣＯ２排出量、呼吸商和窒息点。结果表明,同一种类不同大小和不同种类及生活习性不同,耗氧率、ＣＯ２排出量、呼吸商和窒息点有较大的差别。日本对虾、刀额新对虾的耗氧率、ＣＯ２排出量比其他两种虾低。墨吉对虾次之,斑节对虾为最高。对ＤＯ忍受能力,刀额新对虾最强,在ＤＯ０．６０ｍｇ／Ｌ才开始出现窒息。斑节对虾次之,在ＤＯ降至０．８８ｍｇ／Ｌ出现窒息。墨吉对虾在ＤＯ１．１９ｍｇ／Ｌ出现窒息。日本对虾忍受ＤＯ能力最差,在以ＤＯ１．３１ｍｇ／Ｌ出现窒息。     

植酸酶对凡纳滨对虾生长性能和体成分的影响

在凡纳滨对虾饲料中添加不同量微生物植酸酶（0，500，1000，2000U／k），观察虾的存活率、增重率、饲料系数及虾体和虾壳成分，研究饲料中添加植酸酶对凡纳滨对虾生长性能的影响。结果表明：饲养8周后，饲料中添加500-2000U／kg植酸酶对凡纳滨对虾增重率、存活率和饲料利用元显著影响（P〉0．05）；植酸酶添加各组与对照组虾体的水分、粗蛋白、脂肪、灰分、总钙和总磷含量差异不显著；虾壳中粗灰分和钙含量在500和1000U／k组显著高于对照组和2000U／kg组（P〈0．05），虾壳磷含量在各饲料组间差异不显著。血清磷浓度在对照组和2000U／kg组显著高于500U／kg组（P〈0．05），血清钙浓度在组间差异不显著，血清碱性磷酸酶活性以对照组最高，显著高于500和1000U／kg组。结果说明，在特定试验条件下，饲料中添加植酸酶对凡纳滨对虾幼虾的虾壳和血清成分有显著影响（P〈0．05），对幼虾生长性能影响不显著（P〉0．05）。     

斑节对虾白斑综合症杆状病毒感染的实验模式

在建立斑节对虾实验室养殖模式的基础上．对野外采集的有白斑综合症病毒(WSSV)病典型症状的斑节对虾初步纯化其病毒．并进行WSSV的PCR检测，得到WSSV的感染用样品。对健康斑节对虾分别进行浸浴感染，投喂感染和注射感染。对感染死亡个体进行WSSA的PCR检测和细菌检测，证实WSSV感染性和致死性。浸浴感染、投喂感染和注射感染的感染量分别为4mL／L、0．2g／10g虾体、1／2稀释液0．05mL／10g虾体，死亡开始时间分别为16d、42h、28h，三种感染模式最终死亡率100％．从开始死亡到全部死亡延续时间分别为15d、82h、44h。     

4种对虾耗氧率,CO2排出量,呼吸商和窒息点的研究

较系统研究了斑节对虾、墨吉对虾、日本对虾和的对虾道路 对虾的耗氧率、ＣＯ２排出量,呼吸商和窒息点。结果表明,同一种类不同大小和不同种类及生活习性不同,耗氧率、ＣＯ２排出量、呼吸商和窒息点有较大的差别。日本对虾、刀额新地虾的耗氧率、ＣＯ２排出量比其他两种虾低。墨吉对虾次之,斑节对虾为最高,对ＤＯ忍受能力,刀额新对虾最强,在ＤＯ０．６０ｍｇ／Ｌ才开始出现窒息,斑节对虾闪之在ＤＯ降至０．８８ｍｇ／Ｌ出现     

不同形式钴对凡纳滨对虾生长和组织钴含量的影响

为研究氨基酸螯合钴对凡纳滨对虾的营养效果,在凡纳滨对虾配合饲料中添加5、10、15 mg/kg的氯化钴和蛋氨酸钴。饲养8周,4周末(饲养中期)称重,8周末称重并采集对虾肝胰脏和肌肉,测定肝胰脏和肌肉中的钴含量。结果表明:钴的添加形式和钴水平不影响对虾0~4周增重率,15 mg/kg蛋氨酸钴显著提高了对虾0~8周增重率,且两种形式的钴之间差异显著(P<0.05)。51、0 mg/kg的蛋氨酸钴和5、101、5 mg/kg的氯化钴不影响8周末对虾的增重率。特定生长率、饲料系数和蛋白质效率的变化趋势与增重率类似。钴的添加形式和钴水平不影响肌肉中的钴含量,15 mg/kg的氯化钴组肝胰脏中钴含量显著高于对照组(P<0.05),其他各组间无显著差异;蛋氨酸钴各组肝胰脏中钴含量无显著差异;钴的添加形式不影响肝胰脏中钴含量。统计结果表明,促进生长方面,两种钴之间存在显著差异,蛋氨酸钴的营养效果好于氯化钴。饲料中蛋氨酸钴的添加量为15 mg/kg时,对虾生长最快,5 mg/kg时,可满足需要。     

潮上带斑节对虾高产养殖试验

１９９０年在潮上带３ｍ的大梅沙养殖场选２８口共１０９００２．４ｍ２虾池进行斑节对虾高产养殖试验。采用提水式供水、虾池小型化、投苗高密度，配备增氧机，精心管养。试验结果获得总产６９５７７．３ｋｇ，平均０．６４ｋｇ／ｍ２，最高１．０１ｋｇ／ｍ２。潮上带提水式高密度精养斑节对虾，具备条件是确实可行的。     

4种斑节对虾亲虾饵料蛋白质的营养价值评价

分析了沙蚕、缢蛏、拖鱿鱼胴体、花蟹蟹肉以及斑节对虾亲虾肌肉和成熟卵巢基本营养成分、氨基酸组成。通过计算4种饵料与斑节对虾亲虾肌肉和成熟卵巢的必需氨基酸比率（A／E）的比值和必需氨基酸指数（EAAI），评价4种斑节对虾亲虾饵料蛋白质的营养价值。结果表明：沙蚕、缢蛏、拖鱿鱼胴体、花蟹蟹肉以及斑节对虾（Penaeus monodon）亲虾肌肉和成熟卵巢中的粗蛋白质量分数分别为9．18％、8．14％、12．10％、17．00％、21．90％、18．60％；总氨基酸质量分数分别为7．30％、7．47％、11．70％、14．80％、19．40％、14．60％。以斑节对虾亲虾肌肉蛋白为参比，沙蚕、缢蛏、拖鱿鱼胴体以及花蟹蟹肉的必需氨基酸指数分别0．93、0．94、0．94、0．97；以斑节对虾亲虾成熟卵巢蛋白为参比，沙蚕、缢蛏、拖鱿鱼胴体以及花蟹蟹肉的必需氨基酸指数分别0．95、0．93、0．88、0．84。这说明4种亲虾饵料相对斑节对虾亲虾而言是一种优质蛋白质，能够满足亲虾蛋白需求。不过，精氨酸、组氨酸等必需氨基酸．不能完全满足亲虾需求。     

蜕壳促长素对斑节对虾、刀额新对虾蜕壳和生长的作用

在对虾人工配合饲料中添加蜕（脱）壳促长素，喂养斑节对虾（ＰｅｎａｅｕｓｍｏｎｏｄｏｎＦａｂｒｉｃｉｕｓ）和刀额新对虾（ＭｅｔａｐｅｎａｅｕｓｅｎｓｉｓＤｅＨａａｎ），观察其促进蜕壳、生长的作用。试验证明：饲料中添加不同浓度（０．５‰。、１．０‰、１．５‰）的蜕壳促长素，均不同程度地促进对虾的蜕壳和生长。添加浓度为１．５％。效果最为明显。值得在生产中应用。     

南海历史地理争端空间分布与关联性研究

南海占我国海洋国土的2/3,不仅是中国国家安全的天然屏障和重要的出海口与战略通道,而且是我国未来重要的能源接续区与资源基地、地缘政治经济问题的多发区。对南海争端发展态势进行空间分布与关联性GIS分析,是重要的辅助决策支持。结果表明：（1）文献研究和网络爬虫等方法可以快速有效地获取南海争端历史地理数据,GIS技术能够对南海争端历史地理数据进行时空变化的深入分析;（2）就争端事件的时间分布而言,事件数量随时间推进呈现显著增长,个别发生重大南海争端事件的年份出现突增的现象;（3）就争端事件的空间分布而言,与越南及菲律宾相关的南海争端事件尤甚,其他周边国家则相对较少,这主要源于越菲两国的战略利益与南海所在区域交叠,使得越菲不断挑起事端;（4）越南、菲律宾与马来西亚等国的利益诉求区主要位于南沙群岛,各国诉求区分布明确,局部交错。网络GIS技术丰富了历史地理研究的途径,为其提供了新的思路和方法。     

南海北部陆架区海域深水金线鱼的产卵场

根据 1997年 12月至 1999年 6月在南海北部陆架区海域进行的底拖网渔业资源调查的资料 ,统计分析了南海北部陆架区海域深水金线鱼性腺成熟期的组成及分布、季节性变化和水深变化规律。结果表明 ,南海北部陆架区海域深水金线鱼的产卵场位于广东沿海水深 6 0～ 15 0m海区 ,尤其是珠江口外海区的群体更为密集 ;该生殖群体的产卵期较长 ,且分布范围大 ,未见有显著性变化。     

A new species and new recorded species of Lumbrineridae Schmarda, 1861 (Annelida: Polychaeta) from China

Based on material deposited in the Marine Biological Museum of the Chinese Academy of Sciences(Qingdao),a new species,Lumbrineris sinensis sp.nov.,as well as Augeneria albidentata(Ehlers,1908),which is recorded for the first time from coastal water of China,are reported in the present paper.The specimens examined were collected during 1958 to 1960 from the Yellow Sea,East China Sea and South China Sea at depths of 4 to 182 m.     

Interdecadal variability of the South China Sea monsoon and its relationship with latent heat flux in the Pacific Ocean

We analyzed interdecadal variability of the South China Sea monsoon and its relationship with latent heat flux in the Pacific Ocean, using NCEP wind field and OAFlux heat flux datasets. Results indicate that South China Sea monsoon intensity had an obvious interdecadal variation with a decreasing trend. Variability of the monsoon was significantly correlated with latent heat flux in the Kuroshio area and tropical Pacific Ocean. Variability of latent heat flux in the Kuroshio area had an interdecadal increasing trend, while that in the tropical Pacific Ocean had an interdecadal decreasing trend. Latent heat flux variability in these two sea areas was used to establish a latent heat flux index, which had positive correlation with variability of the South China Sea monsoon. When the latent heat flux was 18 months ahead of the South China Sea monsoon, the correlation coefficient maximized at 0.58 (N=612), with a 99.9% significance level of 0.15. Thus, it is suggested that latent heat flux variability in the two areas contributes greatly to interdecadal variability of the South China Sea monsoon.     

Sedimentary Characteristics of the Second Marine Layer During the Late Marine Isotope Stage 3 in Southern Yellow Sea and Their Response to the East Asian Monsoon

We use the particle size of sediments in core YS01 A to study the sedimentary environment of the mud deposit in the central South Yellow Sea of China during late Marine Isotope Stages 3(MIS3;40.5 kyr–31.3 kyr).In addition,the East Asian Monsoon and its relationship with the North Atlantic Ocean climate change are discussed based on the sensitive grain-size calculation and the spectrum analysis.The results show that during late MIS3,the muddy area in the central South Yellow Sea experienced the evolution of coastal facies,shallow marine facies,coastal facies,and continental facies,with weak hydrodynamic conditions.Compared with other climate indicators,we found that there were many century to millennium-scale climate signals documented in the muddy area sediments in the central South Yellow Sea.According to our particle size results,three strong winter monsoon events occurred at 37.6 kyr,35.6 kyr and 32.2 kyr.The East Asian Winter Monsoon records in core YS01 A are consistent with the Greenland ice core and the Hulu cave stalagmite δ~(18)O.The millennial and centennial scale cycles,which are 55 yr,72 yr,115 yr,262 yr respectively,correspond to solar activity cycles,while the 1049 yr and 2941 yr cycles correspond to the Dansgaard-Oeschger cycles.These cycles indicate that the paleoclimate evolution of the area was controlled by the solar activities,with the high-latitude driving thermohaline circulation as the main energy conveyor belt,followed by the sea-air-land amplification of the winter monsoon variation in the central Yellow Sea in the late MIS3.     

Study on numerical simulation and dynamic mechanism of winter-time circulation in the eastern China seas

An MOM2 based 3-dimentional prognostic baroclinic Z-ordinate model was established to study the circulation in eastern China seas, considering the topography, inflow and outflow on the open boundary, wind stress, temperature and salinity exchange on the sea surface. The results were consistent with observation and showed that the Kuroshio intrudes in large scale into the East China Sea continental shelf East China, during which its water is exchanged ceaselessly with outer sea water along Ryukyu Island. The Tsushima Warm Current is derived from several sources, a branch of the Kuroshio, part of the Taiwan Warm Current, and Yellow Sea mixed water coming from the west of Cheju Island. The water from the west of Cheju Island contributes approximately 13% of the Isushima Warm Current total transport through the Korea Strait. The circulation in the Bohai Sea and Yellow Sea is basically cyclonic circulation, and is comprised of coastal currents and the Yellow Sea Warm Current. Besides simulation of the real circulation, numerical experiments were conducted to study the dynamic mechanism. The numerical experiments indicated that wind directly drives the East China Sea and Yellow Sea Coastal Currents, and strengthens the Korea Coastal Current and Yellow Sea Warm Current. In the no wind case, the kinetic energy of the coastal current area and main YSWC area is only 1% of that of the wind case.Numerical experiments also showed that the Tsushima Warm Current is of great importance to the formation of the Korea Coastal Current and Yellow Sea Warm Current.     

南海地区岩石圈有效弹性厚度估计

分析了测高卫星同时应用于重力模型和地形模型时对计算南海地区岩石圈有效弹性厚度的影响,该影响可能会高估有效弹性厚度值。对比多项研究认为,南沙群岛地区岩石圈的有效弹性厚度约为9 km。通过对南海地区岩石圈的有效弹性厚度分布进行计算发现,南海海盆的有效弹性厚度最小约为4 km,南海周边的有效弹性厚度值较高,与海底岩石圈年龄的空间分布有较强的相关性,符合南海海盆扩张的历史。     

Study on the Correlation Between Chlorophyll Maximum and Remote Sensing Data

Based on the in situ optical measurements in the Bohai Sea of China, which belongs to a typical case-2 water area, we studied the characteristics of DCM （deep chlorophyll maximum） such as its spatial distribution, vertical profile, etc. We found that when the depth of the chlorophyll maximum is comparatively small, even in turbid coastal water regions, there is always a good correlation between the concentrations of chlorophyll maximum and the satellite-received signals in blue-green spectral bands; the correlation is even better than that between the surface chlorophyll concentrations and the satellite-received signals. The strong correlation existing even in turbid coastal water regions indicates that an ocean color model to retrieve the concentration of DCM can be constructed for coastal waters if a comprehensive knowledge of the vertical distribution of chlorophyll concentration in the Bohai Sea of China is available.     

PRELIMINARY STUDY ON PISIONIDS (ANNELIDA:POLYCHAETA PISIOMDAE) FROM HAINAN ISLAND COASTAL WATERS, SOUTH CHINA SEA

MODUcTIONThislistof4pisionidspedesisoneofthecontributi0nstothestudyoflittofalmeiofaunaIp0lychaetesfromtheChinesecoast.ThernateriaIswerecoforduringashortexpeditiontoHainanIslandbyProfB.L.Wu,Mr.F.P.HuangandMr.Z.H.ixngoftheFirstInstituteofdrinography,StatedrinicAdndnisttationinQingdaoandProfDr.W.WeStheideandDr.G.PurschkeoftheUniversityofDenabruckinOct0berl99l,additionalmateriaIsweresamPledbyMr.Z.H.Dinginash0rtexpeditiontoHongkonginJanuary1995.Hithert0,onlyPtrionelevisetoSaZhao…