趋磁细菌基因组和磁小体成链机制研究进展

趋磁细菌能够形成纳米级的磁小体,磁小体排列成链作为生物磁针使细菌沿氧化还原梯度在地磁线上移动。磁小体独特的晶体和磁性特征使其在生物技术、医药和地质生物学应用等领域有巨大的潜力,磁小体的细胞生物学特征也使其成为原核生物细胞器研究的理想模型。本文介绍了近年来在趋磁细菌基因组和磁小体链形成机制方面的研究进展。     

黄海冷水团附近沉积物中的趋磁细菌及磁小体的特性研究

对黄海冷水团附近海域的沉积物进行了富集、荧光显微镜观察、电镜观察,并对其中大小均匀、形状规则的高密度颗粒进行了能谱分析。结果显示,在黄海冷水团附近有趋磁细菌和磁小体的存在。     

海洋趋磁细菌研究进展

海洋趋磁细菌是近年来研究的热点之一,趋磁细菌和磁小体的研究具有重要的理论意义和应用价值。本文对海洋趋磁细菌的研究进行了回顾和展望。1 简介 趋磁细菌(Magnetotactic bacterium)是一类能够沿着磁力线运动的特殊细菌。趋磁细菌最早是1975年     

生物磁性纳米材料——磁小体培养优化及形成过程研究

采用正交试验的方法优化磁小体培养条件,结果使趋磁细菌OD600达到0.440(生物量为1.166×109cells/ml),同时运用磁收集传代法,使趋磁细菌Cmag值稳定在1.9—2.0。培养时用透射电镜观察磁小体形成过程,结果发现培养24h细菌体内已有较小晶体形成且沿长轴分布;48h晶体长大且形成分段链沿长轴排列;72h晶体进一步成熟仍以分段链沿长轴排列;随后细菌逐渐衰亡磁小体变小,168h可见部分自溶细菌中仍有磁小体链;192h细菌自溶磁小体链分散到环境中。还观察到磁小体在细菌分裂时采用两种分离方式,一种为磁小体分配到两个子细胞;另一种为磁小体只分配到一个子细胞。无磁小体的子细胞,一部分逐渐产生磁小体,另一部分不产生磁小体。     

三亚天涯海角潮间带趋磁细菌特性

在三亚天涯海角潮间带沉积物中发现了一定量的海洋趋磁细菌,最大丰度可达100个/cm3。透射电子显微镜(TEM)观察发现该区域趋磁细菌绝大多数为趋磁球菌。磁小体形状单一,皆为棱柱形;磁小体排列方式多样,以链状排列为主,包括单链、双链,也有少数成簇排列。高分辨率透射电子显微镜(HRTEM)与X-射线能谱仪(EDXS)结果表明,磁小体成分为Fe3O4。趋磁细菌的铁含量平均为2.05%(0.70%~7.53%)。通过16S r RNA基因文库的构建与测序,在78个阳性克隆中得到了16个趋磁细菌序列,分属9个OTU。系统发育分析结果表明,其中8个OTU属于α-变形菌纲,1个OTU属于γ-变形菌纲。在全部9个OTU中,有8个OTU与已知趋磁细菌序列的相似性低于97%,有5个OTU与已知趋磁细菌序列的相似性低于93%,说明该区域存在可观的趋磁细菌资源。     

荣成月湖潮间带单细胞趋磁细菌的多样性研究

Marine magnetotactic bacteria were collected from the intertidal sediments of Yuehu Lake(China), where their abundance reached 103–104 ind./cm3. Diverse morphotypes of magnetotactic bacteria were observed, including cocci and oval, vibrio-, spirillum-, rod-, elliptical-, handle- and bar-shaped forms. The magnetococci were the most abundant, and had flagella arranged in parallel within a bundle. The majority of magnetosomes were arranged in one, two or multiple chains, although irregular arrangements were also evident. All the results of high-resolution transmission electron microscopy(HRTEM) analysis show that magnetosome crystals were composed of Fe3O4, and their morphology was specific to particular cell morphotypes. By the 16 S r RNA gene sequence analysis, we found fourteen operational taxonomic units(OTUs) which were related to magnetotactic bacteria. Among these, thirteen belonged to the Alphaproteobacteria and one to the Gammaproteobacteria.Compared with known axenic and uncultured marine magnetotactic bacteria, the 16 S r RNA gene sequences of most magnetotactic bacteria collected from the Yuehu Lake exhibited sequence identities ranging from 90.1% to96.2%(97%). The results indicate that microbial communities containing previously unidentified magnetotactic bacteria occur in the Yuehu Lake.     

青岛潮间带趋磁细菌的垂直分布特征及与环境因子的关系

趋磁细菌是一类能够沿着磁力线运动的革兰氏阴性细菌, 主要生活在淡水和海洋的有氧-无氧过渡区。本调查通过对青岛汇泉湾潮间带沉积物柱状样分层样品中趋磁细菌的计数和沉积物理化因子的分析, 研究了趋磁细菌的垂直分布特征及其与环境因子的关系。结果显示趋磁细菌以趋北型的趋磁球菌占优势, 主要分布环境的氧化还原电位为179.7～-107.0 mV, 为弱还原-还原性质, 最大丰度(94～169个/cm³)出现在氧化还原跃层(表层下5～9cm黄-黑沙界面处), 暗示有氧-无氧过渡区; 趋磁细菌的垂直变化趋势与粒径、含水率、硫酸盐的变化趋势基本一致。     

基于高通量测序方法分析荣成月湖潮间带趋磁细菌多样性

荣成月湖是一个典型天然潟湖,潮间带沉积物中存在多种形态趋磁细菌。通过Roche454高通量测序平台,对沉积物样品(B_S)和磁收样品(B_M)进行16SrRNA基因高通量测序,认识荣成潮间带沉积物的细菌群落结构,并了解趋磁细菌多样性及系统进化地位。研究结果表明沉积物样品中主要的细菌类群为δ-变形菌纲,占总细菌数的26.4%,其次是γ-变形菌纲和α-变形菌纲;而磁收样品中细菌多样性和种类明显降低,以α-变形菌纲占绝对优势,相对比例达72.6%。在磁收样品和沉积物样品分别发现了1 612条和186条reads与趋磁细菌相关,分别占细菌总数的5.76%和0.85%,磁收样品中趋磁细菌数是沉积物样品的6.8倍。对两个样品中获得的趋磁细菌序列进行系统进化分析,发现这些序列多数属于变形菌门的α-变形菌纲,以趋磁球菌占绝对优势,少数属于δ-变形菌纲,与多细胞趋磁原核生物亲缘关系最近。海洋趋磁螺菌属仅在B_M样品中检出,趋磁弧菌属在B_M样品优势度高于B_S样品,而多细胞趋磁原核生物和趋磁螺菌属在B_S样品中优势度更高。通过分析样品间的差异OTUs,认为荣成潮间带沉积物中可能存在大量未知的趋磁细菌新类群。研究结果为下一步培养和开发趋磁细菌这一功能菌群,发现趋磁细菌新类群及趋磁细菌生态功能提供了基础资料。     

青岛海洋趋磁球菌QH-3 的鞭毛特征

趋磁细菌(magnetotactic bacteria,MTB)是一类能够沿着磁力线运动的特殊细菌。MTB在胞内磁小体的导向下借助自身鞭毛进行趋磁趋氧运动,以到达适宜的生境。本研究在青岛潮间带沉积物中发现大量的趋磁球菌,通过光镜与透射电镜对其运动方式与显微结构进行观察。结果显示优势趋磁细菌为卵球形,大小为2.5μm×2.1μm,宽长比为0.85,将其命名为QH-3。QH-3为趋北型(North-seeking)MTB,运动速度88μm/s,细胞内两侧各有一条由棱柱形颗粒组成的磁小体链。QH-3在菌体同一侧有两个由直径62 nm±9 nm的鞘包裹7根鞭毛组成的鞭毛束。这种特殊的鞭毛结构与已报道的致病菌的不同,而与法国地中海趋磁球菌MO-1的相似。     

海底管道磁法探测技术研究

磁法探测是海底管道检测的重要手段,为了根据磁测数据准确地判断管道的位置,从海底管道的磁场模型出发,研究了海底管道的磁异常特征,并提出了一种根据磁测数据准确识别管道的方法:对于对称状的磁异常曲线,其极值即为管道的位置;对于非对称状的磁异常曲线,对其求一阶梯度,位于极大值和极小值之间的梯度极值点即为管道的位置。海底管道实际磁法测量应用实践表明,所提方法是有效可行的。     

荔湾3-1外输海底管道中落管抛石技术

落管抛石是进行深水抛石的一种工程技术,可作为保证管道稳定性、处理悬跨、抑制隆起屈曲和进行跨越支撑的工程解决方案。系统研究了抛石在波流作用下的稳定性,落石对管道冲击的影响以及施工技术。针对南海环境条件下的抛石稳定理论进行了修正;分析了三层聚丙烯涂层管道在落石冲击下的可接受抛石粒径;提出同时结合石块稳定性结果和管道抗冲击性能要求下的石料分级的方法;最后,对该技术在荔湾3-1外输海底管道的工程应用进行详细阐述。     

FY-1C/1D全球海上气溶胶业务反演算法研究

介绍了基于FY-1C/1D气象卫星数据进行全球海洋气溶胶反演的业务算法,主要论述了海上气溶胶反演的单通道算法基本原理.该算法的核心内容是建立气溶胶光学厚度查算表,该查算表基于6S辐射传输模式,在假定气溶胶模式条件下,卫星的表观反射率是卫星观测几何(太阳卫星角)和气溶胶光学厚度函数,最终由卫星观测表观反射率反演出气溶胶光学厚度.业务反演过程中还考虑到数据质量检验、云检测处理和太阳耀斑区去除等.利用该算法对FY-1C卫星自2001年起的部分资料进行反演试验,并在FY-1D卫星发射后投入业务应用,自2002年8月开始能实时得到每天和每月的全球气溶胶光学厚度产品.从两颗卫星两年多连续反演结果分析可以非常清晰地发现全球气溶胶主要排放源地和全球海上气溶胶分布的季节变化,与国外卫星反演结果十分接近.     

蛋白质精氨酸甲基转移酶1调控DNA损伤修复和细胞凋亡

蛋白质精氨酸甲基转移酶1 (protein arginine methyltransferases 1, PRMT1)是PRMT家族中的重要一员,属于Ⅰ型PRMTs,细胞内超过85%的蛋白质精氨酸的甲基化由其催化。PRMT1通过调节底物的甲基化水平,从而调控蛋白的功能及蛋白参与的细胞活动和生理过程,包括细胞信号转导,DNA损伤修复,转录调控,RNA代谢,蛋白质相互作用等。本文着重讨论PRMT1的结构、底物及其在DNA损伤修复和细胞凋亡中的功能。     

Teleparallel equivalent theory of (1+1)-dimensional gravity

A theory of (1+1)-dimensional gravity is constructed on the basis of the teleparallel equivalent of general relativity. The fundamental field variables are the tetrad fields e_i^μ and the gravity is attributed to the torsion. A dilatonic spherically symmetric exact solution of the gravitational field equations characterized by two parameters M and Q is derived. The energy associated with this solution is calculated using the two-dimensional gravitational energy--momentum formula.     

MOS-1/1b MESSR Observations of the Antarctic Sea Ice: Ice Bands and Ice Streamers

Meso- or submeso-scale features of the Antarctic sea ice are investigated using the MOS-1/1b MESSR Images (spatial resolution of approximately 50 m) received at Syowa Station. Particular attention is paid to the ice bands and ice streamers in coastal polynyas. In the Antarctic Ocean, ice bands can be often seen not only at the ice edge but also in the ice interior zone throughout the year and they extend for hundreds of kilometers in the latitudinal direction. It is found that the width and spacing of ice bands tend to decrease from winter to summer. The width of ice band is about 2–6 km in August and September, and 0.1–0.7 km in December. The spacing of ice bands is about 3–10 km in August and September, and 0.1–2 km in December. In coastal polynyas, ice streamers, which are composed of new ice, are sometimes observed. In general, the row of the streamers is spaced at 0.5–2 km with a width of 0.1–1.0 km.     

南堡1—5海上导管架批量优快钻井技术

为加快南堡1号构造的开发速度,冀东油田在南堡1—5海上导管架尝试实施了批量钻井技术。针对南堡1—5海上导管架井口间距小的特点,对井口布置、优选及轨迹设计进行了深入研究和论证,并对施工顺序、工艺作了精细安排,保证了钻井施工的顺利,提高了钻井速度。南堡1～5井组5口井平均钻井周期为16．72d,较未实施批量钻井单井钻井周期缩短30％以上。该项技术为缓解海上钻井平台紧张被动局面提供了一个行之有效的解决方案,同时为人工岛模块钻机高效利用提供了新思路。     

SSA1—1型声学水位在长江口潮位站的应用

本文介绍了长江口区水文泥沙概况，举例说明了ＳＳＡ１－１型声水位计在崇头潮位站比测试验和投产应用情况，结果表明该水位计是收集长江口区潮位资料较理想的仪器。     

东方1-1气田外输管线(路由区)腐蚀环境探讨

本文根据1997年2月国家海洋局第一海洋研究所和广州二海海洋勘察公司对北部湾南部东方1-1气田海底管线路由区海水及沉积物腐蚀性因子调查的资料，概述了该海区电阻率、溶解氧、Fe^3 /Fe^2 、Eh、有机质等腐蚀因子的特征。对腐蚀环境进行了综合分析，指出调查区海水腐蚀情况与临近海区海水接近，各站表层海土腐蚀性偏向于较强级，从而提出了防腐蚀对策。     

SRAP标记在马氏珠母贝家系F1代中的分离

用SRAP(sequence-related amplified polymorphism,序列相关扩增多态性)标记首次对马氏珠母贝(Pinctada martensii)红壳家系的亲本及其子一代(F1)进行了遗传分析.7对引物共扩增出63个位点,多态位点比例为68.25%,其中正常位点59个(93.65%),异常位点4个(6.35%).59个正常位点中,20个位点在F1不分离,39个位点分离.卡方检验表明,39个分离位点中符合孟德尔分离规律的位点28个,占分离位点数的71.79%;偏孟德尔分离规律的位点11个,占分离位点数的28.21%.符合孟德尔分离规律的位点中按1:1分离的位点占25%,3:1分离的位点占75%,1:1分离位点所占比例较小,因此要利用马氏珠母贝家系F1进行SPAP遗传图谱的绘制应适当增加引物数量或改用杂交家系.     

Comparison of the Ku-Band Range Noise Level and the Relative Sea-State Bias of the Jason-1, TOPEX,and Poseidon-1 Radar Altimeters Special Issue: Jason-1 Calibration/Validation

TOPEX/Poseidon is a well known success, with the operational altimeter (TOPEX) and the experimental one (Poseidon-1), providing data of unprecedented quality. However, there are two major differences between the TOPEX and Poseidon-1 radar altimeters on board TOPEX/Poseidon. The first is related to the estimated range noise; the second is linked to the sea-state bias (SSB) model estimates. Since the recent launch of the Jason-1 radar altimeter (also called Poseidon-2), we have been cross-comparing these three systems to better characterize each of them. Analyzing standard user products, we have found that Jason-1 is behaving like Poseidon-1 and thus shows the same observed differences when compared with TOPEX. A comparative analysis of their features was performed, starting from the on-board acquisition of the ocean return and ending with the ground generation of the high level accuracy oceanographic product. The results lead us to believe that the sources for these differences lie in both the waveform tracking processing and the presence or abscence of a retracking procedure whether on-board or on ground. Because Poseidon-1 and Jason-1 waveforms are retracked while TOPEX waveforms are not in the products distributed to the users, we have applied the same ground retracking algorithm to the waveforms of the three radar altimeters to get consistent data sets. The analysis of the outputs has shown that: (a) the noise level for the three radar altimeters is definitively the same, and (b) the source of the relative SSB between Jason-1 and TOPEX lies in the different behavior of the on-board tracking softwares.