Constraints on the use of phytoplankton as a biological quality element within the Water Framework Directive in Portuguese waters

The European Union Water Framework Directive (WFD), a new regulation aiming to achieve and maintain a clean and well-managed water environment, refers to phytoplankton as one of the biological quality elements that should be regularly monitored, and upon which the reference conditions of water quality should be established. However, the use of phytoplankton as a biological quality element will result in several constraints, which are analyzed in this article with examples from Portuguese waters. Specifically, the establishment of reference conditions of water quality may be difficult in some water bodies for which no historical data exists. The sampling frequency proposed for phytoplankton monitoring does not seem suitable to assess phytoplankton succession, and may preclude the detection of algal blooms. Finally, the use of chlorophyll a as a proxy of phytoplankton biomass and abundance has been proposed by some authors, but it may overlook blooms of pico- and small nanophytoplankton, and overestimate the importance of large microphytoplankton. Furthermore, most studies in Portugal have used only inverted microscopy for phytoplankton observation and quantification; this method does not permit the distinction between autotrophic and heterotrophic cells, especially in samples preserved with Lugol's solution, and does not allow the observation of smaller-sized cells. Finally, some techniques, such as remote sensing and chemotaxonomic analysis, are proposed to be used as supplements in phytoplankton monitoring programs.     

The use of benthic indicators in Europe: from the Water Framework Directive to the Marine Strategy Framework Directive

The Water Framework Directive (WFD) and the Marine Strategy Framework Directive (MSFD) are the European umbrella regulations for water systems. It is a challenge for the scientific community to translate the principles of these directives into realistic and accurate approaches. The aim of this paper, conducted by the Benthos Ecology Working Group of ICES, is to describe how the principles have been translated, which were the challenges and best way forward. We have tackled the following principles: the ecosystem-based approach, the development of benthic indicators, the definition of ‘pristine’ or sustainable conditions, the detection of pressures and the development of monitoring programs. We concluded that testing and integrating the different approaches was facilitated during the WFD process, which led to further insights and improvements, which the MSFD can rely upon. Expert involvement in the entire implementation process proved to be of vital importance.     

A revisitation of TRIX for trophic status assessment in the light of the European Water Framework Directive: application to Italian coastal waters

The trophic status classification of coastal waters at the European scale requires the availability of harmonised indicators and procedures. The composite trophic status index (TRIX) provides useful metrics for the assessment of the trophic status of coastal waters. It was originally developed for Italian coastal waters and then applied in many European seas (Adriatic, Tyrrhenian, Baltic, Black and Northern seas). The TRIX index does not fulfil the classification procedure suggested by the WFD for two reasons: (a) it is based on an absolute trophic scale without any normalization to type-specific reference conditions; (b) it makes an ex ante aggregation of biological (Chl-a) and physico-chemical (oxygen, nutrients) quality elements, instead of an ex post integration of separate evaluations of biological and subsequent chemical quality elements. A revisitation of the TRIX index in the light of the European Water Framework Directive (WFD, 2000/60/EC) and new TRIX derived tools are presented in this paper. A number of Italian coastal sites were grouped into different types based on a thorough analysis of their hydro-morphological conditions, and type-specific reference sites were selected. Unscaled TRIX values (UNTRIX) for reference and impacted sites have been calculated and two alternative UNTRIX-based classification procedures are discussed. The proposed procedures, to be validated on a broader scale, provide users with simple tools that give an integrated view of nutrient enrichment and its effects on algal biomass (Chl-a) and on oxygen levels. This trophic evaluation along with phytoplankton indicator species and algal blooms contribute to the comprehensive assessment of phytoplankton, one of the biological quality elements in coastal waters.     

Palaeoecology, reference conditions and classification of ecological status: the EU Water Framework Directive in practice

The European Union's Water Framework Directive (WFD) requires that all Member States within the European Union determine reference conditions for aquatic ecosystems to provide a baseline against which to measure the effects of past and present activities. Reference conditions are subsequently used to classify the ecological status of European waters. The decisions regarding environmental status will be important future elements in the management of European coastal waters. We have developed a number of classification scenarios for total nitrogen (TN) in the overlying waters of the southern part of Roskilde Fjord, Denmark, taking as our basis a palaeoecological reconstruction of fluctuations in TN between 1850 and 1995. We present a provisional classification scheme for the ecological status of Roskilde Fjord, sensu the WFD. Decision(s) regarding the deviation from reference conditions will give a wide range of apparent ecological status from good, through moderate and poor, to bad depending upon the definition of an acceptable deviation from reference conditions. The determination of an acceptable deviation will ultimately be a political decision, and will result in a wide range in the protection of coastal waters in Europe. There is still, however, an urgent need for a sound scientific documentation of the various scenarios for the implementation of the WFD.     

Assessing reference conditions and physico-chemical status according to the European Water Framework Directive: a case-study from the Basque Country (Northern Spain)

The European Water Framework Directive requires the development of new and accurate methodologies, addressing the assessment of the physico-chemical status of transitional and coastal waters; these are considered by the Directive as the supporting elements for the final evaluation of the Ecological Quality Status.

This contribution develops new approaches in the determination of the physico-chemical status, solving some problems detected in previous contributions, i.e.: (a) fitting the classification of water bodies and typologies, by means of the stretching of the typologies, according to the natural salinity gradient of types; (b) defining reference conditions, based upon the new approach to typologies, (c) proposing accurate multivariate methodologies, in determining the physico-chemical status of the transitional and coastal waters, based upon the defined typologies and references; and (d) discussion of the results obtained by reference to methodological aspects and water quality evolution in the Basque Country, Spain (as a case-study), during the last decade.     

Towards the application of the Water Framework Directive in Italy: Assessing the potential of benthic tools in Adriatic coastal transitional ecosystems

The potential of four benthic indices (AMBI/M-AMBI, BENTIX, BITS) was assessed in Italian coastal transitional ecosystems. The community composition showed a strong dominance of lagoonal, tolerant species, and out of more of 400 species found, only about 40 taxa were dominant. The full agreement of the four indices on an undegraded (Good or better) or degraded (Moderate or worse) status occurred only in 32.3% of stations. This study evidenced that BENTIX is inappropriate for eutrophic Adriatic lagoons, and that in such environments M-AMBI classification is actually too much dependent on diversity and richness, and seems unable to capture some peculiarities of benthic assemblages in transitional waters. AMBI and BITS gave similar classifications despite the different level of taxonomic identification needed. The unmodified use of these indices might impair accurate assessment of ecological quality status and decision-making on the managers’ point of view.     

Abiotic Stream Classification as a Basis for a Surveillance Monitoring Network in Austria in Accordance with the EU Water Framework Directive

This study presents the 26 major surface water types established in Austria in accordance with the draft of the EU Water Framework Directive (WFD). These types are made up of so‐called aquatic landscape units and large rivers. The 17 aquatic landscape units were defined using a database in which all Austrian running waters with a catchment area greater than 10 km^[2] were described according to the following typological features: size of catchment area, altitude of catchment area and confluences, stream order, geology, zoogeographical regions (ecoregions), and subregions. At running waters with gauges, a classification according to flow regimes was carried out. Large rivers were defined as running waters with a stream order ⩾7 and/or a catchment area > 2500 km² and/or with an average flow >50 m³/s. These major types represent, inter alia, the basis for the establishment of a surveillance monitoring network as required by the WFD.     

High‐frequency water quality monitoring in an urban catchment: hydrochemical dynamics,primary production and implications for the Water Framework Directive

This paper describes the hydrochemistry of a lowland, urbanised river‐system, The Cut in England, using in situ sub‐daily sampling. The Cut receives effluent discharges from four major sewage treatment works serving around 190 000 people. These discharges consist largely of treated water, originally abstracted from the River Thames and returned via the water supply network, substantially increasing the natural flow. The hourly water quality data were supplemented by weekly manual sampling with laboratory analysis to check the hourly data and measure further determinands. Mean phosphorus and nitrate concentrations were very high, breaching standards set by EU legislation. Although 56% of the catchment area is agricultural, the hydrochemical dynamics were significantly impacted by effluent discharges which accounted for approximately 50% of the annual P catchment input loads and, on average, 59% of river flow at the monitoring point. Diurnal dissolved oxygen data demonstrated high in‐stream productivity. From a comparison of high frequency and conventional monitoring data, it is inferred that much of the primary production was dominated by benthic algae, largely diatoms. Despite the high productivity and nutrient concentrations, the river water did not become anoxic, and major phytoplankton blooms were not observed. The strong diurnal and annual variation observed showed that assessments of water quality made under the Water Framework Directive (WFD) are sensitive to the time and season of sampling. It is recommended that specific sampling time windows be specified for each determinand, and that WFD targets should be applied in combination to help identify periods of greatest ecological risk. © 2015 The Authors. Hydrological Processes published by John Wiley & Sons Ltd.     

Assessment of the ecological status of Mediterranean French coastal waters as required by the Water Framework Directive using the Posidonia oceanica Rapid Easy Index: PREI

This paper describes the PREI (Posidonia oceanica Rapid Easy Index), a method used to assess the ecological status of seawater along Mediterranean French coasts. The PREI was drawn up according to the requirements of the Water Framework Directive (WFD 2000/60/EC) and was tested on 24 and 18 stations in PACA (Provence-Alpes-Côtes d’Azur) and Corsica, respectively. The PREI is based on five metrics: shoot density, shoot leaf surface area, E/L ratio (epiphytic biomass/leaf biomass), depth of lower limit, and type of this lower limit. The 42 studied stations were classified in the first four levels of status: high, good, moderate and poor. The PREI values ranged between 0.280 and 0.847; this classification is in accordance with our field knowledge and with our knowledge of the literature. The PREI was validated regarding human pressure levels (r² = 0.74). (http://eurex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2000:327:0001:0072:EN:PDF).     

Application of bioassays in toxicological hazard, risk and impact assessments of dredged sediments

Given the potential environmental consequences of dumped dredged harbour sediments it is vital to establish the potential risks from exposure before disposal at sea. Currently, European legislation for disposal of contaminated sediments at sea is based on chemical analysis of a limited number of well-known contaminants for which maximum acceptable concentrations, action levels (ALs), have been set. The present paper addresses the issue of the applicability of in vitro and in vivo bioassays for hazard, risk and local impact assessment of dredged polluted sediments to be disposed of at sea. It discusses how and to what extent selected bioassays can fill in the gaps left open by chemical analysis and the way in which the bioassays may contribute to the present licensing system for disposal. Three different purposes for application were distinguished: the most basic application (A) is a rapid determination of the hazard (potential toxicity) of dredged sediments which is then compared to ALs in a licensing system. As with chemical analysis on whole sediment extracts, the bioavailability of the chemicals is not taken into account. As in vitro assays with sediment extracts are not sensitive to matrix effects, a selection of specific in vitro bioassays can be suitable fast and standardized additions for the licensing system. When the outcome of (A) does not convincingly demonstrate whether the sediment is clean enough or too polluted, further bioanalysis can help the decision making process (B). More aspects of the mostly unknown complex chemical mixtures are taken into account, including the bioavailability and chronic toxicity focusing on ecologically relevant endpoints. The ecotoxicological pressure imposed by the dredged sediments can be quantified as the potentially affected fraction (PAF) based on chemical or biological analysis of levels of contaminants in sediment or biota. To validate the predicted risk, the actual impact of dumped harbour sediments on local ecosystems (C) can be determined using a dedicated set of in vitro and in vivo bioassays as well as bio-indicators selected based on the information obtained from (A) and (B) and on the characteristics of the local ecosystem. Conversely, the local sediment impact assessment (C) can direct fine-tuning of the selection of chemical and bioassay analyses and for setting safe levels in the licensing system. It is concluded that in vitro and in vivo bioassays and biological indicators are useful tools in the process of hazard, ecotoxicological risk and impact assessment of dredged harbour sediments, provided they are consciously chosen and quality criteria for assay performance are defined.     

Enzymatic and cellular responses in relation to body burden of PAHs in bivalve molluscs: A case study with chronic levels of North Sea and Barents Sea dispersed oil

Mytilus edulis and Chlamys islandica were exposed to nominal dispersed crude oil concentrations in the range 0.015–0.25 mg/l for one month. Five biomarkers (enzymatic and cellular responses) were analysed together with bioaccumulation of PAHs at the end of exposure. In both species, PAH tissue residues reflected the exposure concentration measured in the water and lipophylicity determined the bioaccumulation levels. Oil caused biomarker responses in both species but more significant alterations in exposed C. islandica were observed. The relationships between exposure levels and enzymatic responses were apparently complex. The integrated biomarker response related against the exposure levels was U-shaped in both species and no correlation with total PAH body burden was found. For the monitoring of chronic offshore discharges, dose- and time-related events should be evaluated in the selection of biomarkers to apply. From this study, cellular damages appear more fitted than enzymatic responses, transient and more complex to interpret.     

Quality assessment of the blue mussel (Mytilus edulis): Comparison between commercial and wild types

This study compared species identity, microplastics, chemical and microbial contamination between consumption mussels and wild type mussels, collected at Belgian department stores and Belgian groynes and quaysides, respectively. Species identification based on genetic analysis showed a high number of Mytilus (M.) edulis compared to M. galloprovincialis and M. edulis/galloprovincialis hybrid mussels. The number of total microplastics varied from 2.6 to 5.1 fibres/10 g of mussel. A higher prevalence of orange fibres at quaysides is related to fisheries activities. Chemical contamination of polycyclic aromatic hydrocarbons and polychlorobiphenyls could be related to industrial activities and water turbidity, with maximum concentrations at the quayside of port Zeebrugge. The inverse was noted for Escherichia coli contamination, which was relatively low at Zeebrugge quayside with a total count of 3.9 × 10² CFU/100 g tissue, due to limited agricultural effluents. Results of this complementary analysis stress the importance of integrated monitoring and quality assessment.     

Ecological status of seagrass ecosystems: An uncertainty analysis of the meadow classification based on the Posidonia oceanica multivariate index (POMI)

Quantifying the uncertainty associated with monitoring protocols is essential to prevent the misclassification of ecological status and to improve sampling design. We assessed the Posidonia oceanica multivariate index (POMI) bio-monitoring program for its robustness in classifying the ecological status of coastal waters within the Water Framework Directive. We used a 7-year data set covering 30 sites along 500 km of the Catalonian coastline to examine which version of POMI (14 or 9 metrics) maximises precision in classifying the ecological status of meadows. Five factors (zones within a site, sites within a water body, depth, years and surveyors) that potentially generate classification uncertainty were examined in detail. Of these, depth was a major source of uncertainty, while all the remaining spatial and temporal factors displayed low variability. POMI 9 matched POMI 14 in all factors, and could effectively replace it in future monitoring programs.     

Chemical contamination of the Hudson-Raritan Estuary as a result of the attack on the World Trade Center: analysis of polycyclic aromatic hydrocarbons and polychlorinated biphenyls in mussels and sediment

The September 11, 2001 attack on the World Trade Center (WTC) resulted in a massive plume of dust and smoke that blanketed lower Manhattan and part of the Hudson-Raritan Estuary (HRE). The NOAA National Status and Trends Mussel Watch Program has long-term monitoring sites in the area and thus had an opportunity to assess the effect of the WTC attack on PAH and PCB contamination of the surrounding estuary. Seven additional sites were added in the Upper HRE to attain higher sampling resolution for comparison with regularly sampled Mussel Watch Project HRE sites. Elevated background levels of PCBs and PAHs in mussel tissue and sediments were high enough before the WTC attack that concentrations were not measurably changed by WTC derived contaminant input.     

Implications of EU-Water Framework Directive for the East German Postmining Landscape Lausitz: Coping with a sparse knowledge of the underground

Mining dumps are major sources of pollutants within the mining area of Lausitz, especially sulphate and iron. Their existence in catchment areas comprising groundwater bodies or lakes often imposes negative effects on the water quality. The European Union Water Framework Directive [EU-WFD, 2000. Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy. The European Parliament and Council, L327/1, p. 72] aims to achieve ‘good quality’ status for all water bodies across Europe by 2015. Consequently, predicting the development of ground and lake water quality is necessary and must be based on the geochemical composition of the mining dumps. Therefore, the dumps need to be quantified as pollutant sources.A method to calculate the amount of sulphate in mine dumps is presented. It is based on historic geological and geochemical data characterising the pre-mining situation. Additional information on the dump body, derived from the vertical extension of mining activities and the current Digital Elevation Model (DEM), allows the composition of the dump to be determined. This procedure is demonstrated for the Bärwalde site. An average total sulphur content of 0.62% (5.9 million tonnes) was calculated for the Bärwalde dump. About 40% of it is estimated to be pyrite sulphur. Applying an average pyrite oxidation rate for the whole dump body of 7% led to an additional water-soluble mass of 0.18 million tonnes of sulphate sulphur.Applying this technique to all mine dumps managed by the postmining administration company LMBV, will improve our knowledge of the catchment area for the different lakes of Lausitz and will form an essential basis for reactive transport calculations.     

Application of biomarkers for assessing the biological impact of dredged materials in the Mediterranean: the relationship between antioxidant responses and susceptibility to oxidative stress in the red mullet (Mullus barbatus)

In the period 1997-2000, approximately 1,800,000 m3 of material dredged from the Port of Leghorn was discharged into a sea dumping site located 14 miles from the coast. The red mullet (Mullus barbatus) was used as a bioindicator species for monitoring the biological impact of these discharges on a geographical and temporal scale. Organisms were sampled over three years (1998-2000) at different stations and several biomarkers, both of exposure and effect, were analyzed. Bioavailability of specific classes of pollutants was evaluated by analyzing levels of metallothioneins, the activity of cytochrome P450 1A (CYPIA) and of glutathione S-transferases. Among biomarkers of effect, special attention was paid to the balance between prooxidant challenge and antioxidant defenses, and to the appearance of damage caused by oxidative stress. The analyses of the main components of the antioxidant system included superoxide dismutase, catalase, glutathione peroxidases, glutathione reductase, glyoxalase I and II, and total glutathione. These data were integrated with the measurement of total oxyradical scavenging capacity (TOSC) as an indication of the overall biological resistance to toxicity of different forms of oxyradicals (peroxyl radicals, hydroxyl radicals and peroxynitrite). Results indicated a biological impact in organisms sampled near the disposal site; the impact was particularly evident during 1999 and mainly related to organic chemicals such as PAH. Exposure to these pollutants also caused variations in the levels and activity of several antioxidants. The analysis of TOSC, however, revealed that the overall capacity of specific tissues of organisms to absorb various oxidants was not seriously compromised when challenged with increased prooxidant pressures. Variations of single antioxidants were useful in revealing early warning "biological responses", while integration with TOSC analyses indicated if such changes also reflect a more integrated and functional "biological effect" with possible consequences at the organisms level. The red mullet appears to be a useful sentinel species for a biomarker approach to monitoring impact caused by dredged materials.     

Effects of chronic exposure to dispersed oil on selected reproductive processes in adult blue mussels (Mytilus edulis) and the consequences for the early life stages of their larvae

Mussels (Mytilus edulis) were continuously exposed to dispersed crude oil (0.015-0.25 mg/l) for 7 months covering the whole gamete development cycle. After 1 month exposure to 0.25 mg oil/l, the level of alkali-labile phosphates (ALP) and the volume density of atretic oocytes in female gonads were higher than those in the gonads of control females, indicating that oil affected the level of vitellogenin-like proteins and gamete development. Spawning of mussels was induced after 7 months oil exposure. Parental oil exposure did not affect subsequent fertilization success in clean seawater but this was reduced in 0.25 mg oil/l. Parental exposure to 0.25 mg oil/l caused both slow development and a higher percentage of abnormalities in D-shell larvae 2 days post-fertilization; reduced growth 7 days post-fertilization. These effects were greatly enhanced when larval stages were maintained at 0.25 mg oil/l. Similar studies are warranted for risk assessment prognosis.     

Catchment-scale vulnerability assessment of groundwater pollution from diffuse sources using the DRASTIC method: a case study

Abstract

The catchment-scale groundwater vulnerability assessment that delineates zones representing different levels of groundwater susceptibility to contaminants from diffuse agricultural sources has become an important element in groundwater pollution prevention for the implementation of the EU Water Framework Directive (WFD). This paper evaluates the DRASTIC method using an ArcGIS platform for assessing groundwater vulnerability in the Upper Bann catchment, Northern Ireland. Groundwater vulnerability maps of both general pollutants and pesticides in the study area were generated by using data on the factors depth to water, net recharge, aquifer media, soil media, topography, impact of vadose zone, and hydraulic conductivity, as defined in DRASTIC. The mountain areas in the study area have “high” (in 4.5% of the study area) or “moderate” (in 25.5%) vulnerability for general pollutants due to high rainfall, net recharge and soil permeability. However, by considering the diffuse agricultural sources, the mountain areas are actually at low groundwater pollution risk. The results of overlaying the maps of land use and the groundwater vulnerability are closer to the reality. This study shows that the DRASTIC method is helpful for guiding the prevention practices of groundwater pollution at the catchment scale in the UK.

Citation Yang, Y. S. & Wang, L. (2010 Yang, Y. S. and Wang, L. 2010. A review of modelling tools for implementation of the EU Water Framework Directive in handling diffuse water pollution. Water Resour. Manage., 24: 1819–1843.  [Google Scholar]) Catchment scale vulnerability assessment of groundwater pollution from diffuse sources using the DRASTIC method: a case study. Hydrol. Sci. J. 55(7), 1206–1216.