三丁基锡对罗氏沼虾血清中免疫酶活力的影响

将罗氏沼虾(Macrobrachium rosenbergii)在含0.1、0.2、0.4 mg/L三丁基锡(TBT)的水体中浸浴48 h,检测TBT对罗氏沼虾血清中部分免疫酶活力的影响。结果表明:12 h内高剂量(0.4 mg/L)可诱导超氧化物歧化酶(SOD),酶活力差异有统计学意义(P0.01),至48 h时,诱导作用逐步解除;12 h内各剂量组血清中酸性磷酸酶(ACP)活力均表现抑制效应,酶活力差异有统计学意义(P0.05),而高剂量组在48 h时TBT对ACP表现为诱导效应,酶活力高于对照组,差异有统计学意义(P0.05);低剂量组(0.1 mg/L)TBT对罗氏沼虾血清中AKP活力的影响无统计学意义(P0.05),而中剂量组(0.2 mg/L)和高剂量组(0.4 mg/L)AKP活力表现为先抑制后逐渐转为诱导的效应,。     

Acute toxicity of organochlorine insecticide endosulfan to the giant freshwater prawn Macrobrochium rosenbergii

Endosulfan, an organochlorine pesticide, is highly toxic and effective at controlling pests in agriculture, horticulture, and public health programs. In this study, static bioassays were used to evaluate the toxicity of endosulfan to freshwater prawns( Macrobrachium rosenbergii) of various lengths(1.5±0.03,4±0.08, and 7±0.06 cm). Additionally, the activities of peroxidase(POD), acid phosphatase(ACP),alkaline phosphatase, acetylcholinesterase(AChE), and Na + /K +-ATPase were analyzed to refl ect the effects of endosulfan exposure. The 96 h LC 50 of endosulfan for prawns 1.5, 4, and 7 cm long were 1.86, 4.53,and 6.09μg/L, respectively, improved tolerance to endosulfan with growth. The POD activities of test organisms exposed to low concentrations of endosulfan were inhibited, indicating the presence of oxygen damaged tissue. Moreover, a notable decrease in AChE activity was observed due to overstimulation of neurotransmission, which might result in abnormal behavior. The effect caused by endosulfan on phosphatase production in the hepatopancreas of prawns 1.5, 4, and 7 cm long was different because the ability of nonspecifi c immune regulation increased with growth. The 96 h LC 50 values obtained in this study could be used in the formulation of water-quality criteria in China. Moreover, the changes in enzymes activities of M. rosenbergii under stress of endosulfan could be applied in the establishment of early warning indicators for bio-safety.     

The Evaluation on Biological Properties of Carboxymethylchitosan and Carboxymethyl-chitin

Carboxymethyl-chitosan and carboxymethyl-chitin were prepared with the methods developed in our laboratory. The DS （degree of substitution） and DD （degree of deacetylation） of the carboxymethyl-chitosan were 103.14% and 97.18% respectively, while the DS of the carboxymethyl-chitin was 96.37%. Their effects on human fibroblasts, intradermal irritation test, in vitro and vivo degradability, and biocompatibility were evaluated. The results indicate that the polysaccharides at low concentrations can facilitate the growth of human fibroblasts and the carboxymethyl-chitosan at 100 μg mL^-1 is the most effective. The polysaccharides at higher concentrations, however, inhibit the growth of fibroblasts. The PII （Primary Irritation Index） values of CM-chitosan and CM-chitin are both 0.0, which shows that they have no irritation reaction. Both of the polysaccharides show good degradability and biocompatibility. Carboxymethyl-chitin degrades faster in vitro than carboxymethyl-chitosan. The latter, however, has no inflammatory reaction after being implanted in vivo for 7 d and shows better biocompatibility. This study may provide a scientific basis for the use of earboxymethyl-ehitosan and carboxymethyl-chitin as biomaterials.     

交通路况基态修正模型及其效率分析

 交通路况在时间上和空间上具有连续变化的特征,在时空维度上对交通路况进行高分辨率采样得到的数据,对研究交通路况的时空动态十分有利。但长时间大范围的高分辨率交通路况信息数据量巨大,给数据的组织和管理带来了困难。目前,尚没有一种成熟的时空数据模型对高时空分辨率交通路况数据进行高效(顾及数据存储与访问效率)的组织管理。本文提出一种基于线性参照系统的交通路况基态修正模型。此模型应用基态修正模型的基本思想,在时间维度上对交通路况数据进行无损压缩,又引入动态分段技术和线性参照系统,以路划作为交通路况载体,在空间维度上对交通路况数据进行压缩存储。利用成都市区真实交通路况数据,本文验证了此模型的有效性,比较了6种不同参数下交通路况基态修正模型的存储和访问效率,给出了最佳模型建议。     

Large eddy simulation of the rotation effect on the ocean turbulence kinetic energy budget in the surface mixed layer

A non-hydrostatic, Boussinesq, and three-dimensional large eddy simulation(LES) model was used to study the impact of the Earth's rotation on turbulence and the redistribution of energy in turbulence kinetic energy(TKE) budget. A set of numerical simulations was conducted,(1) with and without rotation,(2) at different latitudes(10°N, 30°N, 45°N, 60°N, and 80°N),(3) with wave breaking and with Langmuir circulation, and(4) under different wind speeds(5, 10, 20, and 30 m/s). The results show that eddy viscosity decreases when rotation is included, indicating that rotation weakens the turbulence strength. The TKE budget become tight with rotation and the effects of rotation grow with latitude. However, rotation become less important under Langmuir circulation since the transport term is strong in the vertical direction. Finally, simulations were conducted based on field data from the Boundary Layer and Air-Sea Transfer Low Wind(CBLAST-Low) experiment. The results, although more complex, are consistent with the results obtained from earlier simulations using ideal numerical conditions.     

Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations

Small heat shock proteins encompass a widespread but diverse class of proteins,which play key roles in protecting organisms from various stressors.In the present study,the full-length cDNAs of two small heat shock proteins(MgsHSP22 and MgsHSP24.1)were cloned from Mytilus galloprovincialis,which encoded peptides of 181 and 247 amino acids,respectively.Both MgsHSP22 and MgsHSP24.1 were detected in all tissues examined by real-time PCR,with the highest expression being observed in muscle and gonad tissues.The real-time PCR results revealed that Cd signifi cantly inhibited MgsHSP22 expression at 24 h and MgsHSP24.1 at 24 and 48 h under 5 μg/L Cd 2 + exposure.MgsHSP24.1 expression was also signifi cantly inhibited after 50 μg/L Cd 2+ exposure for 48 h.With regard to antioxidant enzymes,increased GPx and CAT activity were detected under Cd 2+ stress(5 and 50 μg/L),while no signifi cant difference in SOD activity was observed throughout the experiment.Overall,both MgsHsps and antioxidant enzymes revealed their potential as Cd stress biomarkers in M.galloprovincialis.     

Quantitative assessment of remotely sensed global surface models using various land classes produced from Landsat data in Istanbul

Digital elevation model(DEM) is the most popular product for three-dimensional(3D) digital representation of bare Earth surface and can be produced by many techniques with different characteristics and ground sampling distances(GSD). Space-borne optical and synthetic aperture radar(SAR) imaging are two of the most preferred and modern techniques for DEM generation. Using them, global DEMs that cover almost entire Earth are produced with low cost and time saving processing. In this study, we aimed to assess the Satellite pour l'observation de la Terre-5(SPOT-5), High Resolution Stereoscopic(HRS), the Advanced Space-borne Thermal Emission and Reflection Radiometer(ASTER), and the Shuttle Radar Topography Mission(SRTM) C-band global DEMs, produced with space-borne optical and SAR imaging. For the assessment, a reference DEM derived from 1∶1000 scaled digital photogrammetric maps was used. The study is performed in 100 km2 study area in Istanbul including various land classes such as open land, forest, built-up land, scrub and rough terrain obtained from Landsat data. The analyses were realized considering three vertical accuracy types as fundamental, supplemental, and consolidated, defined by national digital elevation program(NDEP) of USA. The results showed that, vertical accuracy of SRTM C-band DEM is better than optical models in all three accuracy types despite having the largest grid spacing. The result of SPOT-5 HRS DEM is very close by SRTM and superior in comparison with ASTER models.     

A MODIS time series data based algorithm for mapping forest fire burned area

Burned area mapping is an essential step in the forest fire research to investigate the relationship between forest fire and climate change and the effect of forest fire on carbon budgets. This study proposed an algorithm to map forest fire burned area using the Moderate-Resolution Imaging Spectroradiameter (MODIS) time series data in Heilongjiang Province, China. The algorithm is divided into two steps: Firstly, the ‘core’ pixels were extracted to represent the most possible burned pixels based on the comparison of the temporal change of Global Environmental Monitoring Index (GEMI), Burned Area Index (BAI) and MODIS active fire products between pre- and post-fires. Secondly, a 15-km distance was set to extract the entire burned areas near the ‘core’ pixels as more relaxed conditions were used to identify the fire pixels for reducing the omission error as much as possible. The algorithm comprehensively considered the thermal characteristics and the spectral change between pre- and post-fires, which are represented by the MODIS fire products and the spectral index, respectively. Tahe, Mohe and Huma counties of Heilongjiang Province, China were chosen as the study area for burned area mapping and a time series of burned maps were produced from 2000 to 2011. The results show that the algorithm can extract burned areas more accurately with the highest accuracy of 96.61%.     

Molecular diversity of Enteromorpha from the coast of Yantai: a dual-marker assessment

We collected nine Enteromorpha specimens from the coast of Yantai and evaluated their diversity based on analyses of their ITS （internal transcribed spacer） and 5S rDNA NTS （non-transcribed spacer） sequences. The ITS sequences showed slight nucleotide divergences between Enteromorpha linza and Enteromorpha prolifera. In contrast, multiple highly variable regions were found in the ITS region of Enteromorpha flexuosa. In general, there were more variable sites in the NTS region than in the ITS region in the three species. The variations in 5S rDNA NTS sequences indicated that the molecular diversity of Enteromorpha from the coast of Yantai is very high. However, a phylogenetic tree constructed using 5S rDNA NTS sequence data indicated that genetic differences were not directly related to geographical distribution.     

潮滩表层沉积物含水量的高光谱预测模型与反演分析

潮滩土壤含水量具有变化频率快、空间变化大的特征,是影响潮滩地表反射率的重要因素。潮滩土壤含水量的精确提取,可为潮滩特征地物信息遥感反演提供基础。本文利用江苏大丰王港潮滩4种典型沉积物、449组不同含水量对应的实测光谱曲线数据进行特征分析,构建高光谱预测模型,实现了潮滩沉积物含水量的遥感反演。研究结果表明:(1)在短波红外波段,沉积物含水量与反射率之间存在良好的分段线性相关关系,分段点对应的含水量分别为42%和62%;(2)1165nm、1336nm、1568nm和1780nm特征波段反射率,对含水量变化具有良好响应,由特征波段组合计算得到的差值水指数DWI、比值水指数RWI和归一化水指数NDWI与含水量呈显著线性相关,可有效改善单波段反射率与含水量之间的分段线性关系;(3)3个水指数中,DWI反演的含水量精度优于RWI和NDWI,且对不同含水量大小均有良好适应性,而RWI和NDWI更适合含水量变化范围中等的情况;(4)对于粉砂、砂质粉砂、粉砂质砂和砂4种沉积物类型,DWI1336,1780验证组模拟含水量与实测含水量的相关系数,分别为0.891、0.915、0.920和0.905,均方根误差分别为9.87%、3.56%、4.24%和2.98%,表明由DWI构建的高光谱遥感反演模型,可有效实现潮滩表层含水量的时空变化预测。     

Numerical simulation of wave field in the South China Sea using WAVEWATCH III

Wave fi elds of the South China Sea(SCS) from 1976 to 2005 were simulated using WAVEWATCH III by inputting high-resolution reanalysis wind fi eld datasets assimilated from several meteorological data sources. Comparisons of wave heights between WAVEWATCH III and TOPEX/Poseidon altimeter and buoy data show a good agreement. Our results show seasonal variation of wave direction as follows: 1. During the summer monsoon(April–September), waves from south occur from April through September in the southern SCS region, which prevail taking about 40% of the time; 2. During the winter monsoon(December–March), waves from northeast prevail throughout the SCS for 56% of the period; 3. The dominant wave direction in SCS is NE. The seasonal variation of wave height H s in SCS shows that in spring, H s ≥1 m in the central SCS region and is less than 1 m in other areas. In summer, H s is higher than in spring. During September–November, infl uenced by tropical cyclones, H s is mostly higher than 1 m. East of Hainan Island, H s 2 m. In winter, H s reaches its maximum value infl uenced by the north-east monsoon, and heights over 2 m are found over a large part of SCS. Finally, we calculated the extreme wave parameters in SCS and found that the extreme wind speed and wave height for the 100-year return period for SCS peaked at 45 m/s and 19 m, respectively, SE of Hainan Island and decreased from north to south.     

福建省森林生态系统NPP的遥感模拟与分析

利用MODIS遥感影像,结合气象资料等数据,采用BEPS过程模型对2004年福建省的森林生态系统植被净初级生产力(NPP)进行了模拟验证。研究结果表明,2004年福建省森林生态系统NPP平均值为578.97gC/m²·a,NPP总量累计达到46.18×106tC;不同林地NPP全年平均值大小依次为:竹林≈阔叶林>杉木>马尾松,其值分别为:788.6gC/m²·a,780.0gC/m²·a,519.8gC/m²·a,437.3gC/m²·a;时空分析结果表明,2004年6-8月NPP形成较为明显的"坑"形分布形态,主要的原因之一很可能是有效降水量偏少;在空间分布上,福建省森林生态系统NPP与海拔高程显著相关,体现了该地区森林生态系统NPP空间分布的地域特征,这在一定程度上表明随着海拔上升,山高坡陡,人类对森林生态系统的干扰活动减少,有助于森林生态系统生产力的提高和维持。最后,分析了应用BEPS过程模型模拟福建省森林生态系统净初级生产力的不确定性问题。     

A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis

The high phenotypic plasticity in the shell of oysters presents a challenge during taxonomic and phylogenetie studies of these economically important bivalves. However, because DNA can exhibit marked differences among morphologically similar species, DNA barcoding offers a potential means for oyster identification. We analyzed the complete sequences of the cytochrome oxidase subunit I （COI） of five common Crassostrea species in China （including Hong Kong oyster C. hongkongensis, Jinjiang oyster C. ariakensis, Portuguese oyster C. angulata, Kumamoto oyster C. sikamea, and Pacific oyster C. gigas） and screened for distinct fragments. Using these distinct fragments on a high-resolution melting analysis platform, we developed an identification method that does not rely on species-specific PCR or fragment length polymorphism and is efficient, reliable, and easy to visualize. Using a single pair of primers （Oyster- COI-1）, we were able to successfully distinguish among the five oyster species. This new method provides a simple and powerful tool for the identification of oyster species.     

Remote forcing of Indian Ocean warming on Northwest Pacific during E1 Nino decaying years： a FOAM model approach

This paper attempts to analyze in detail the remote influence of the Indian Ocean Basin warming on the Northwest Pacific （NWP） during the year of decaying E1 Nifio. Observation data and the Fast Ocean- Atmosphere coupled Model 1.5 were used to investigate the triggering conditions under which the remote influence is formed between the positive sea surface temperature （SST） anomaly in the North Indian Ocean and the Anomalous Northwest Pacific anticyclone （ANWPA）. Our research show that it is only when there is a contributory background wind field over the Indian Ocean, i,e., when the Indian Summer Monsoon （ISM） reaches its peak, that the warmer SST anomaly in the North Indian Ocean incites significant easterly wind anomalies in the lower atmosphere of the Indo-West tropical Pacific. This then produces the remote influence on the ANWPA. Therefore, the SST anomaly in the North Indian Ocean might interfere with the prediction of the East Asia Summer Monsoon in the year of decaying E1 Nifio. Both the sustaining effect of local negative SST anomalies in the NWP, and the remote effect of positive SST anomalies in the North Indian Ocean on the ANWPA, should be considered in further research.     

���ö�̬PPP�������ZTD��Ƶ�仯

??????PPP????????IGS?ο????????????ж??PPP???????????????PPP??ZTD?????????????PPP??ZTD??IGS??????ZTD?ο???????????????PPP???????????????????????30 s????????t???????????????<6 mm????     

A thin cloud removal method from remote sensing image for water body identification

In this paper,a thin cloud removal method was put forward based on the linear relationships between the thin cloud reflectance in the channels from 0.4 μm to 1.0 μm and 1.38 μm.Channels of 0.66 μm,0.86 μm and 1.38 μm were chosen to extract the water body information under the thin cloud.Two study cases were selected to validate the thin cloud removal method.One case was applied with the Earth Observation System Moderate Resolution Imaging Spectroradiometer(EOS/MODIS) data,and the other with the Medium Resolution Spectral Imager(MERSI) and Visible and Infrared Radiometer(VIRR) data from Fengyun-3A(FY-3A).The test results showed that thin cloud removal method did not change the reflectivity of the ground surface under the clear sky.To the area contaminated by the thin cloud,the reflectance decreased to be closer to the reference reflectance under the clear sky after the thin cloud removal.The spatial distribution of the water body area could not be extracted before the thin cloud removal,while water information could be easily identified by using proper near infrared channel threshold after removing the thin cloud.The thin cloud removal method could improve the image quality and water body extraction precision effectively.     

����������GPS�춥�������ӳ���ɽ�ˮ��Ӱ���о�

???????GPS???????????????о???????????GPS???????????????????????о??????????????????????????????????????????????????????У???????????????????????е?????????????????????????????????????????     

Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology

The industrially important organic compound 1,3-propanediol(1,3-PDO) is mainly used as a building block for the production of various polymers. In the present study, response surface methodology protocol was followed to determine and optimize fermentation conditions for the maximum production of 1,3-PDO using marine-derived Klebsiella pneumoniae HSL4. Four nutritional supplements together with three independent culture conditions were optimized as follows: 29.3 g/L glycerol, 8.0 g/L K 2 HPO 4, 7.6 g/L(NH 4) 2 SO 4, 3.0 g/L KH 2 PO 4, pH 7.1, cultivation at 35°C for 12 h. Under the optimal conditions, a maximum 1,3-PDO concentration of 14.5 g/L, a productivity of 1.21 g/(L·h) and a conversion of glycerol of 0.49 g/g were obtained. In comparison with the control conditions, fermentation under the optimized conditions achieved an increase of 38.8% in 1,3-PDO concentration, 39.0% in productivity and 25.7% in glycerol conversion in flask. This enhancement trend was further confirmed when the fermentation was conducted in a 5-L fermentor. The optimized fermentation conditions could be an important basis for developing lowcost, large-scale methods for industrial production of 1,3-PDO in the future.