Lower Groundwater 14C Age by Atmospheric CO2 Uptake During Sampling and Analysis

Uptake of atmospheric CO₂ during sample collection and analysis, and consequent lowering of estimated ages, has rarely been considered in radiocarbon dating of groundwater. Using field and laboratory experiments, we show that atmospheric CO₂ can be easily and rapidly absorbed in hyperalkaline solutions used for the extraction of dissolved inorganic carbon, resulting in elevated ¹⁴C measurements. Kinetic isotope fractionation during atmospheric CO₂ uptake may also result in decrease of δ¹³C, leading to insufficient corrections for addition of dead carbon by geochemical processes. Consequently, measured ¹⁴C values of groundwater should not be used for age estimation without corresponding δ¹³C values, and historical ¹⁴C data in the range of 1 to 10% modern Carbon should be re‐evaluated to ensure that samples with atmospheric contamination are recognized appropriately. We recommend that samples for ¹⁴C analysis should be collected and processed in the field and the laboratory without exposure to the atmosphere. These precautions are considered necessary even if ¹⁴C measurements are made with an accelerator mass spectrometer.     

Reconstruction of the climate in the Tianmu Mountain area,Zhejiang Province,in the last 160 years by σ<Superscript>13</Superscript>C sequence of tree ring α-cellulose

Based on cross-dating tree rings from the Tianmu Mountain, Zhejiang Province, the tree rings α-cellulose σ¹³C time series was measured. By spectrum analysis, the variation of tree-ring σ¹³C sequence shows a quasi periodicity of 4.4 years, which is coincident with the standard cycle of El Niño. After removing the long-term decrease trend of the σ¹³C variation related to atmospheric CO₂ concentration, the response of the σ¹³C to climate elements was analyzed using meteorology data from a nearby weather station. The results suggest that there is a distinct relativity between high-frequency variation of tree ring σ¹³C series and seasonal climate parameters, e.g. temperature and precipitation, with a significant time-lag effect. In addition, the high frequency also reflects the strength change of the East Asian Monsoon. The multiple regression method was employed to reconstruct the historical climate, and to analyze the climate change and trend in the last 160 years in the northern Zhejiang Province.     

Stable carbon isotope signatures of cellulose in macrophytes collected from three eutrophic lakes in China

Aquatic plants are essential for maintaining the diversity and stability of a lake ecosystem. Stable carbon isotopes (δ¹³C) of macrophytes have been widely used as a powerful tool to study ecological processes and paleoenvironmental evolution in lakes. Varying results are obtained when using the δ¹³C of macrophytes to study the changes in the lake environment at different spatio-temporal scales. Thus, sample preparation and subsequent laboratory analyses are crucial for studying environmental changes using the isotopic signal retained in the macrophytes, and are essential for the interpretation of isotope-environment relationships. This study analyzed the δ¹³C of different tissue components of macrophytes in three lakes of the lower Yangtze River basin, and a correlation analysis was performed on aquatic environments influencing the δ¹³C values in the different tissue components of macrophytes. The test results showed the difference between the δ¹³C values of the whole sample and cellulose. Relative analyses indicated that the major factors contributing to the δ¹³C variability in macrophytes were pH and the concentration of dissolved inorganic carbon (DIC). The δ¹³C of α-cellulose (δ¹³C_AC) is more sensitive to environmental variables than that of the whole sample (δ¹³C_W) and holocellulose (δ¹³C_HC). The results of this study imply that extraction of α-cellulose is a prerequisite for research on the changes in lake environment using δ¹³C of macrophytes. This study aims to provide theoretical and data basis for further research on the environmental and ecological change using stable carbon isotopes of aquatic plants.     

Quantifying the temporal dynamics of wood in large rivers: field trials of wood surveying,dating, tracking,and monitoring techniques

Wood plays an important role in stream ecology and geomorphology. Previous studies of wood in rivers have quantified spatial distributions but temporal dynamics remain poorly documented. The lack of such data is related to limitations of existing methods, especially when applied to large rivers. Five techniques are field‐tested to assess their utility for quantifying the temporal dynamics in rivers: repeated high‐resolution aerial surveys, the measurement of wood physical characteristics as proxies for ¹⁴C dating, passive and active radio frequency identification (RFID) tags, radio transmitters, and video. The spatial distribution of wood is surveyed using aerial imagery with a resolution finer than 0·10 m. The estimation of temporal trends by repeated aerial‐based surveys needs to consider vegetation growth and hiding. Wood residence times can be calculated using ¹⁴C analysis, but the assessment of wood physical characteristics including decay status and wood density offers a cheaper, if less accurate, alternative. Wood resistance to penetration is tested but results are not significant. Radio transmitters are reliable for multi‐year (～5 year) surveys and can be detected at 800 m. Passive RFID tags are limited by a read range of 0·30 m but are reliable for longer term (>5 year) studies. Active RFID tags combine a moderate read range (10–300 m) and low cost with in‐flood detection but require more testing. Video monitoring of wood passing on the surface of a river is successfully implemented. For a single flood on the Ain River (France), wood transport rates are an order of magnitude higher on the rising limb of the hydrograph than on the falling limb. Overall, the techniques improve the ability to gather the data needed to understand wood transfer processes and calibrate budgets of wood in rivers. Copyright © 2009 John Wiley & Sons, Ltd.     

Radiocarbon: A chronological tool for the recent past

The past few hundred years have seen large fluctuations in atmospheric ¹⁴C concentration. In part, these have been the result of natural factors, including the climatic changes of the Little Ice Age, and the Spörer and Maunder solar activity minima. In addition, however, changes in human activity since the middle of the 19th century have released ¹⁴C-free CO₂ to the atmosphere. Moreover, between c. 1955 and c. 1963, atmospheric nuclear weapon testing resulted in a dramatic increase in the concentration of ¹⁴C in the atmosphere. This was followed by a significant decrease in atmospheric ¹⁴C as restrictions on nuclear weapon testing began to take effect and as rapid exchange occurred between the atmosphere and other carbon reservoirs. The large fluctuations in atmospheric ¹⁴C that occurred prior to 1955 mean that a single radiocarbon date may yield an imprecise calibrated age consisting of several possible age ranges. This difficulty may be overcome by obtaining a series of ¹⁴C dates from a sequence and either wiggle-matching these dates to a radiocarbon calibration curve or using additional information on dated materials and their surrounding environment to narrow the calibrated age ranges associated with each ¹⁴C date. For the period since 1955 (the bomb-pulse period), significant differences in atmospheric ¹⁴C levels between consecutive years offer the possibility of dating recent samples with a resolution of from one to a few years. These approaches to dating the recent past are illustrated using examples from peats, lake and salt marsh sediments, tree rings, marine organisms and speleothems.     

Annual growth rings in a sample of Paraná pine (Araucaria angustifolia): Toward improving the 14C calibration curve for the Southern Hemisphere

This work reports the first high-precision ¹⁴C-AMS dating of holocellulose from a Brazilian subtropical species for the period 1927–1997, with the goal to identify suitable Southern American tree species that will serve as benchmarks for improving the calibration of the ¹⁴C time-scale for the Southern Hemisphere (SH). The tree rings analyzed here came from a single tree of Paraná pine (Araucaria angustifolia) growing at 22°50′S, 46°04′W (Camanducaia, Minas Gerais, Brazil). A slight depletion of atmospheric ¹⁴C after 1927 AD was observed, due to the Suess effect. Our ¹⁴C results also showed the rise and rapid decrease of atmospheric ¹⁴C concentrations associated with the detonation of nuclear weapons during the late 50s, and its subsequent uptake by other large C sinks. Current ¹⁴C data can be used for the study of the global carbon cycle, forensic sciences applications, and the determination of the age and growth rate of tropical trees without annual ring patterns. The remarkable overall agreement of our tree-ring/¹⁴C data with the SH Zone 1–2 compilation dataset shows this subtropical tree species' potential to refine the ¹⁴C calibration curve.     

Influence of climatic factors on the past atmospheric content of the C-14 isotope

Reconstructions of solar activity in the past epochs based on information on the past atmospheric content of the cosmogenic ¹⁴C isotope are nowadays actively discussed. The ¹⁴C isotope is generated in the atmosphere of the Earth under the influence of cosmic rays, and its concentration in annual tree rings carries information on the past solar activity. However, the concentration of this isotope in annual tree rings may also be influenced by climatic factors. In the present work, the possible correlation between variations in the ¹⁴C atmospheric content and in the Earth’s global temperature from the late 14th century to the middle of the 19th century is studied. It is shown that variations in global temperature may produce changes in the ¹⁴C atmospheric content and consequently have to be taken into account in reconstructions of the past solar activity.     

A simulation approach to quantify the parameters and limitations of the radiocarbon wiggle-match dating technique

Worldwide, radiocarbon (¹⁴C) wiggle-match dating is increasingly used to produce high-resolution, ‘near-absolute’, chronologies in a range of different contexts, yet the exact properties and limitations of the technique are not well understood. Here we present the results of extensive and systematic simulations that allow the precision limits of wiggle-match dating to be quantified for different time periods. We have also been able to quantify the effect of modeling decisions on possible precision in terms of how many measurements to make, how far apart to space them, and which calibration curve to employ. While recent trends towards large numbers of annually spaced measurements can improve precision, the effect is generally small, except when the dated sequence falls on a plateau in the calibration curve. Finally, we demonstrate that wiggle-matching against an unsmoothed record of atmospheric ¹⁴C can provide better precision than wiggle-matching against the smoothed IntCal20 or SHCal20 curves. We argue, however, that until intra-hemispheric variation in ¹⁴C is better understood, the hemispheric averages provided by IntCal20 and SHCal20 will be the more appropriate datasets for most wiggle-match applications.     

Variations of O/O in plants from temperate peat bogs (Switzerland): implications for paleoclimatic studies

Despite the great potential of peat bogs as climatic archives, to date only few studies have focused on the climatic controls on cellulose isotopic composition in modern bog plants. This study attempts to calibrate plant-climate relationships by sampling a set of modern plant species (both vascular plants and mosses) and bog surface waters along an altitude transect in Switzerland. Isotopic analyses of water samples show that the δ¹⁸O-values of surface bog waters follow the trend of precipitation despite significant scatter in the data set. Detailed sampling of surface waters within one bog shows that δ¹⁸O-values vary widely and are closely related to the micro-topography of the bog surface. More enriched ¹⁸O/¹⁶O ratios in water samples collected from small raised hummocks than the ones collected from hollows are documented in both horizontal and vertical profiles. A δ¹⁸O-δD plot indicates that the process leading to the isotopic enrichment of the uppermost surface waters is evaporation, greater above Sphagnum covered hummocks than above open pools. To investigate the implications of such high variability of source water for plant α-cellulose δ¹⁸O-values, a detailed study of both surface water and α-cellulose δ¹⁸O-values within one site is conducted. The large δ¹⁸O variability observed in surface waters is found to be considerably smoothed in α-cellulose (by a factor of 5-10 depending on the plant species). This indicates that the water used by plant photosynthetic processes reflects the isotopic composition of the average annual precipitation. This points to a source water level for plants of a few decimeters where the variations are smaller than at the air-water interface. The response of the α-cellulose δ¹⁸O to the environmental gradient along the altitude transect varies considerably from species to species. For most of the species studied, the δ¹⁸O-values decrease with altitude, following the trends of δ¹⁸O-values in precipitation and in surface water samples. Some species, the cotton sedge Eriophorum vaginatum and the moss Sphagnum capillifolium, show statistically significant δ¹⁸O relationships to an altitude of −1.8‰/km and −2.9‰/km respectively. However, some other plant species, Calluna vulgaris, Vaccinium uliginosum, Andromeda poliflora, Carex pauciflora, Sphagnum cuspidatum and Sphagnum magellanicum, do not, or only partially, reflect changes in climatic parameters associated with an altitude increase. Furthermore, changes in relative humidity, which are not correlated with altitude, are found to explain a large part of the variability in δ¹⁸O-values for the sedge Carex pauciflora and the moss Sphagnum cuspidatum. Therefore, this study confirms the importance of species-specific studies when interpreting ¹⁸O/¹⁶O ratios of macrofossils along a fossil peat sequence as a record of past climate changes. Our study allows to extend the mechanistic model that isotopically links source water and cellulose to the physiological specificities of sedges and mosses. A comparison of the modeled and calculated net biological fractionation factors for Eriophorum vaginatum and Sphagnum capillifolium reveals that these two species appear to have a more homogeneous leaf reservoir than trees.     

Paleomagnetic results from lakes Victoria and albert,Uganda

Summary Unfortunately most of the cores taken from Salisbury Channel, Lake Victoria which contained discrete pieces of wood suitable for¹⁴C dating were organic-rich and therefore showed a considerable scattering of the remanent magnetic direction plots resulting in poorly defined oscillations. However, the cores from Pilkington Bay as well as core C5 from Salisbury Channel, Lake Victoria and the cores from Lake Albert show well-defined paleodeclination and paleoinclination logs. Several cores from Pilkington Bay and Lake Albert with some¹⁴C control can be used to verify the isochronous correlation of the paleodeclination and paleoinclination oscillations. Core C5, from Salisbury Channel, Lake Victoria, which did not contain any discrete pieces of plant or shell material has been dated by correlation with other cores which had¹⁴C control. This core which shows the best-defined paleodeclination and paleoinclination logs of this study would appear to have bottomed in sediments in excess of 6000 years BP age and with the work of Maley et al. (1990) from the Cameroons provides a complete record of the Holocene for Central Africa.     

Methods and codes for reservoir–atmosphere 14C age offset calculations

Reservoir ¹⁴C age offsets are invaluable tracers for past changes in carbon cycle and oceanic circulation. Reconstruction of reservoir age offsets with time is also required for calibration purposes (reconstruction of atmospheric calibration curve, calibration of non-atmospheric radiocarbon ages). Thus, properly propagating the various uncertainties linked to reservoir age offset is important for proper interpretation. However, approaches for reservoir age offset calculation – especially when considering pairs of reservoir-derived ¹⁴C and calendar ages – are usually not detailed and inadequate for proper propagation of uncertainties. Here, the various ways to properly calculate reservoir age offsets are described with a focus on a new approach when considering pairs of ¹⁴C and calendar ages. This approach maps the calendar age distribution onto the ¹⁴C time scale prior to reservoir age offset calculation – the “uncalibration-convolution process”. R codes computing reservoir age offsets based on available data are presented. Finally, a case study focusing on the reconstruction of the speleothem-atmosphere ¹⁴C age offsets of speleothem ¹⁴C data used in the latest release of the atmospheric calibration curve is discussed.     

An automated method for varve interpolation and its application to the Late Glacial chronology from Lake Suigetsu,Japan

The Lake Suigetsu sediment has been recognised for its potential to create a wholly terrestrial (i.e. non-reservoir-corrected) ¹⁴C calibration dataset, as it exhibits annual laminations (varves) for much of its depth and is rich in terrestrial leaf fossils, providing a record of atmospheric radiocarbon. Microscopic analysis revealed that the varve record is curtailed due to the incomplete formation or preservation of annual laminae, necessitating interpolation. The program for varve interpolation presented here analyses the seasonal layer distribution and automatically derives a sedimentation rate estimate, which is the basis for interpolation, and applies it to complement the original varve count. As the interpolation is automated it largely avoids subjectivity, which manual interpolation approaches often suffer from. Application to the Late Glacial chronology from Lake Suigetsu demonstrates the implementation and the limits of the method. To evaluate the reliability of the technique, the interpolation result is compared with the ¹⁴C chronology from Lake Suigetsu, calibrated with the tree-ring derived section of the IntCal09 calibration curve. The comparison shows that the accuracy of the interpolation result is well within the 68.2% probability range of the calibrated ¹⁴C dates and that it is therefore suitable for calibration beyond the present tree-ring limit.     

Towards radiocarbon calibration beyond 28 ka using speleothems from the Bahamas

We present a new speleothem record of atmospheric Δ¹⁴C between 28 and 44 ka that offers considerable promise for resolving some of the uncertainty associated with existing radiocarbon calibration curves for this time period. The record is based on a comprehensive suite of AMS ¹⁴C ages, using new low-blank protocols, and U–Th ages using high precision MC-ICPMS procedures. Atmospheric Δ¹⁴C was calculated by correcting ¹⁴C ages with a constant dead carbon fraction (DCF) of 22.7 ± 5.9%, based on a comparison of stalagmite ¹⁴C ages with the IntCal04 (Reimer et al., 2004) calibration curve between 15 and 11 ka. The new Δ¹⁴C speleothem record shows similar structure and amplitude to that derived from Cariaco Basin foraminifera (Hughen et al., 2004, 2006), and the match is further improved if the latter is tied to the most recent Greenland ice core chronology (Svensson et al., 2008). These data are however in conflict with a previously published ¹⁴C data set for a stalagmite record from the Bahamas — GB-89-24-1 (Beck et al., 2001), which likely suffered from ¹⁴C analytical blank subtraction issues in the older part of the record. The new Bahamas speleothem ?¹⁴C data do not show the extreme shifts between 44 and 40 ka reported in the previous study (Beck et al., 2001). Causes for the observed structure in derived atmospheric Δ¹⁴C variation based on the new speleothem data are investigated with a suite of simulations using an earth system model of intermediate complexity. Data-model comparison indicates that major fluctuations in atmospheric ?¹⁴C during marine isotope stage 3 is primarily a function of changes in geomagnetic field intensity, although ocean–atmosphere system reorganisation also played a supporting role.     

Atmospheric14C changes resulting from fossil fuel CO2 release and cosmic ray flux variability

A high-precision tree-ring record of the atmospheric¹⁴C levels between 1820 and 1954 is presented. Good agreement is obtained between measured and model calculated 19th and 20th century atmospheric Δ¹⁴C levels when both fossil fuel CO₂ release and predicted natural variations in¹⁴C production are taken into account. The best fit is obtained by using a ?-diffusion model with an oceanic eddy diffusion coefficient of 3 cm²/s, a CO₂ atmosphere-ocean gas exchange rate of 21 moles m^?2 yr^?1 and biospheric residence time of 60 years.For trees in the state of Washington the measured 1949–1951 atmospheric Δ¹⁴C level was20.0±1.2%. below the 1855–1864 level. Model calculations indicate that in 1950 industrial CO₂ emissions are responsible for at least 85% of the Δ¹⁴C decline, whereas natural variability accounts for the remaining 15%.     

Stability and dynamics of beams on winkler elastic foundations

The dynamical properties of a foundation beam on a Winkler soil have been recently discussed by using the power series method¹⁴ and the finite element method.^{15, 17 – 19} The first method can be applied only if the Winkler soil is linearly distributed along the whole span: on the contrary, the second method is more general, but it requires considerable computational efforts. In this paper a recently developed discretization method has been shown to be particularly well suited for dynamic analysis of foundation beams, in the presence of second order destabilizing effects. The procedure is based on the Hamilton principle, and the equation of motion is deduced from the corresponding Lagrange equations. According to the proposed method, the structure is reduced to a set of rigid bars, connected together by elastic cells. The convergence of the method is shown to be quite rapid, so that a small number of Lagrangian coordinates has to be introduced, and the resulting eigenvalue problem can be easily solved. Numerical comparisons are also performed with the available data of the literature, and the agreement is shown to be excellent. Two particularly unusual cases are illustrated, in order to emphasize the method's peculiarities. Finally, the influence of the axial forces on the fundamental vibration frequency is discussed, both for conservative and non-conservative forces.     

Secular non-random variations of cosmogenic carbon-14 in the terrestrial atmosphere

The time dependence of the¹⁴C content of bristlecone pine wood samples dated by their tree rings and grown during the last 8000 years was examined. The¹⁴C values as measured by the La Jolla Radiocarbon Laboratory were used for the investigation.Two different smoothing techniques were used for constructing values for equal time intervals. In this manner the introduction of regularities, that could have resulted from applied mathematical techniques, could be excluded.There is good evidence for non-random features in the power spectrum, in particular for a 200-year periodicity.The regularities in the power spectrum are further indications supporting the assumption that the¹⁴C variations reflect a property of the sun.     

Robust chronologies for landform development

Obtaining chronological control for geomorphological sequences can be problematic due to the fragmentary and non‐sequential nature of sediment and landform archives. The robust analysis of ¹⁴C ages is often critical for the interpretation of these complicated sequences. This paper demonstrates a robust methodology for the ¹⁴C dating of geomorphological sequences using a case study from the lower Ribble valley, northwest England. The approach adopted incorporates using greater numbers of ages, targeting plant macrofossils, obtaining replicate measurements from single horizons to assess the extent of reworking and the use of Bayesian approaches to test models of the relative order of events. The extent of reworking of organic materials and space‐time dynamics of fluvial change means that it is critical that chronological control is sufficiently resourced with ¹⁴C measurements. As a result Bayesian approaches are increasingly important for the evaluation of large data sets. Assessing the conformability of relative order models informed by interpretation of the geomorphology can identify contexts or materials that are out of sequence, and focuses attention on problem materials (reworking) and errors in interpretation (outlier ages). These relative order models provide a framework for the interrogation of sequences and a means for securing probability‐based age estimates for events that occur between dated contexts. This approach has potential value in constraining the sequence of geomorphological development at scales that vary from individual sites to a catchment or region, furthering understanding of forcing and change in geomorphological systems. Copyright © 2008 John Wiley & Sons, Ltd.     

Aluminum incorporation in α-PbO2 type TiO2 at pressures up to 20 GPa

Aluminum incorporation into the high pressure polymorph of TiO₂ with the structure of α-PbO₂ has been studied from 10 to 20 GPa and 1300 °C by XRD, high-resolution ²⁷Al MAS-NMR and TEM. Al-doped α-PbO₂ type TiO₂ can be recovered at atmospheric pressure. Al₂O₃ solubility in α-PbO₂ type TiO₂ increases with increasing the synthesis pressure. The α-PbO₂ type TiO₂ polymorph is able to incorporate up to 35 wt.% Al₂O₃ at 13.6 GPa and 1300 °C, being the substitution of Ti⁴⁺ by Al³⁺ on normal octahedral sites the mechanism of solubility. The transition to the higher pressure TiO₂ polymorph with the ZrO₂ baddeleyite structure, Akaogiite, has not been observed in the quenched samples at room pressure. The microstructure of the recovered sample synthesized at 16 GPa and 1300 °C points to the existence of a non-quenchable aluminum titanium oxide phase at these conditions.     

Resonance capacity method for earthquake response analysis of hysteretic structures

The Resonance Capacity Method is proposed for the earthquake response analysis of hysteretic structures. Resonance Capacity is a physical quantity of structures which is related to the hysteretic energy absorbed by structures in one cycle and is equated to the acceleration, velocity and displacement amplitudes α₀, d₀ and d₀ of earthquake ground motions at resonance.¹ According to the idealized trapezoidal approximation of earthquake ground motions in the logarithmic period–velocity plane as proposed by Veletsos and Newmark,⁸ the Resonance Capacity property applies in each period range, short, medium and long, where α₀, v₀ and d₀ respectively are approximately constant. In the medium range of periods, the energy dissipated in hysteretic loops and the deformation amplitudes of a single-degree system with elasto–plastic force–deformation relationships are calculated for the case of El Centro 1940, 18 May earthquake, by this Resonance Capacity Method. The result is compared with results from conventional numerical response analyses obtained by Berg and Thomaides,¹⁴ Kato and Akiyama¹² and Veletsos and Newmark,⁸ and the general agreement is seen to be good. Therefore, it may be possible to apply this Resonance Capacity Method over the entire range of periods. By means of this method the earthquake response analysis of hysteretic systems can be performed easily, and the hysteretic energy and fatigue characteristics of structures may be taken into account directly, up to the point of fracture.     

Oxygen and hydrogen isotope ratios in tree rings: how well do models predict observed values?

We have measured annual oxygen and hydrogen isotope ratios in the α-cellulose of the latewood of oak (Quercus robur L.) growing on well-drained ground in Norfolk, UK. We compare the observed values of isotope ratios with those calculated using equations that allow for isotopic fractionation during the transfer of oxygen and hydrogen from source water taken by the tree to cellulose laid down in the cambium. The equations constitute a model in which isotopic fractionation occurs during evaporative enrichment within the leaf and during isotopic change between carbohydrates and water in the trunk during cellulose synthesis. From the relationship between isotope ratios in precipitation and α-cellulose, we deduce that the source water used by the tree comprises a constant mixture of groundwater and precipitation, chiefly from the months of May, June and July of the growth year. By selection of isotopic fractionation factors and the degree of isotope exchange within the trunk, we are able to model the observed annual values of oxygen isotope ratios of α-cellulose to a significant level (r=0.77, P<0.01). When we apply the same model to hydrogen isotope ratios, however, we find that, although we can predict the average value over the time series, we can no longer predict the year-to-year variation. We suggest that this loss of environmental signal in the hydrogen isotopes is caused by differences in the kinetic isotope effects of the biochemical reactions involved in the fixation of hydrogen in different positions of the glucose molecule. Owing to these effects, the hydrogen isotope ratios of cellulose can vary in a way not anticipated in current models and hence may induce non-climatic ‘noise’ in the hydrogen isotope time series.