Seasonal Variations in the Forms of Heavy Metal Occurrence in the Kuibyshev Reservoir Waters and Bottom Deposits

Seasonal dynamics of total concentrations and the main forms of occurrence of heavy metals (HM) in the water mass and bottom deposits (BD) are studied in the Priplotinnyi (Near-dam) Pool of the Kuibyshev Reservoir. In the near-bottom and interstitial waters, heavy metals are mostly found in the form of complex compounds with organic and mineral ligands; the proportions of these complexes and the dominant form in the above media change from season to season. The hard skeleton of bottom deposits contains a large store of heavy metals that can be a source of the secondary pollution of water masses.     

Size Fractions of Heavy Metals in Waters

The heavy metal content of river water samples with 12 and 15 mg/l dry matter of seston is increased to 400 … 3900μg/l by the addition of inorganic heavy metal salts, and after three days the distribution of the heavy metals is determined by fractional filtration (8, 3, 1.2, 0.8, 0.65, 0.45 and 0.22 μm pore size). More than 80% of Ni, Co and Mn are found in the fraction <0.22 μm, 60% of Pb and Cu in >8 μm as well as 40 and 25%, resp., in <0.22 μm, Cd, Cr and Zn are found in all fractions, 60 … 80% being in the fraction <0.22 μm, 90% of Fe occurring in the fraction >8 μm. The influence of specific parameters of water quality as pH-value and seston concentration on the distribution requires further investigations.     

The State of Metal Ions in Natural Waters

On the basis of an analysis of literature and of own investigations, the state of metalions in natural waters is described as essential for the properties. There are investigated especially: hydrolysis, polymerization and complexation with humic substances. An analytical method for determining the degree of dispersion and the shares of free and complexed metal-ions as well as their charge and molar mass is presented.     

Demobilization of Heavy Metals from Mine Waters

The present study elucidates the remediation of mine waters by means of geochemical barriers („active barriers“). Active barrier systems (ABS) are developed, which can prevent heavy metal dispersion by introducing the barrier into the mine. Therefore, low‐cost materials are investigated which can remove contaminants by chemical, and/or physical mechanisms from water. The materials investigated are industrial by‐products (e.g. fly ash, red mud, scale arrears), natural wastes (e.g. tree bark), and relatively cheap natural products (bentonite, zeolites). Red mud and fly ash show the best results for the removal of Zn, Cd, Ni, and Mn from model water. Cd seems to have the strongest binding of the investigated metals on the sorption sites. The main mechanisms of metal removal from solution in this case are sorption and precipitation. Both materials have very good sorption and acid neutralization capacities (ANC), but ANC seems to be the determining factor.     

Interaction between Surface and Subsurface Waters: Case Study of a Region in Kazakhstan

The results of field studies are used to establish the factors that control the interaction between surface water and unconfined groundwater in the region, to study the process of their interaction, and to propose empirical relationships for transient flow. Combined model calculations were also made, and their results and other formulas were compared with the results of field studies conducted by other researchers in large river valleys under dry and moderate climate conditions.     

Monitoring Continental Surface Waters by Satellite Altimetry

The monitoring of continental water stages is a requirement for meeting human needs and assessing ongoing climatic changes. However, regular gauging networks fail to provide the information needed for spatial coverage and timely delivery. Although the space missions discussed here were not primarily dedicated to hydrology, 18 years of satellite altimetry have furnished complementary data that can be used to create hydrological products, such as time series of stages, estimated discharges of rivers or volume change of lakes, river altitude profiles or leveling of in situ stations. Raw data still suffer uncertainties of one to several decimeters. These require specific reprocessing such as waveform retracking or geophysical correction editing; much work still remains to be done. Besides, measuring the flow velocity appears feasible owing to SAR interferometer techniques. Inundated surfaces, and the time variations of their extent, are currently almost routinely computed using satellite imagery. Thus, the compilation of the continuous efforts of the scientific community in these various investigative directions, such as recording from space the discharges of rivers or the change in water volume stored in lakes, can be foreseen in the near future.     

Quality Appraisal of Surface Waters on Fennoscandian Territory

Widely applied bioindication methods are analyzed based on the survey data of 1980–1996 for over 400 lakes and 300 watercourses in the northeastern Fennoscandia known to experience anthropogenic impact (toxification, acidification, eutrophication, and thermofication). Representative results of hydrobiological analysis of the quality of surface waters in areas with mixed toxic pollution (or anthropogenic acidification) may be obtained if methods are applied, different from the bioindication methods that are now accepted as standard for Russia, along with methods of biotesting natural waters. The saprotoxibity index, Kola biotic index, acidification estimation scale, biotesting methods were developed and adapted to regional conditions.     

Assessment of Critical Loads of Acid Fallout for Surface Waters

A method for determining the critical loads of acid fallout for surface waters is described. Adaptation of the method for estimating the degree of water acidification in the Kola North is demonstrated in the process of large-scale investigations carried out in this region. The limiting value of the acid-neutralizing capacity of waters needed for preventing the aquatic ecosystems from degradation is substantiated. The loads of acid fallout in 17% of 460 examined water systems are found to exceed the critical limits and create the conditions for further development of adverse processes in water systems.     

MAPGIS软件在矿区重金属元素环境累积效应研究的应用

矿产资源开发引起的重金属污染是人们对资源利用过程中主要环境问题之一。如今,重金属污染的问题已经十分严重,时刻威胁着我们的生活质量。如何通过模拟分析等手段,借助“3S”技术,达到对矿区重金属元素环境累积效应危害的研究分析是当前重要的课题。针对MAPGIS6.7制图工作的一点经验做以总结,旨在使矿区环境污染研究者了解MAPGIS软件在研究重金属环境累积效应中的一点应用。     

Determination of Antibiotic Residues in Manure,Soil, and Surface Waters

In the last years more and more often detections of antimicrobially active compounds (“antibiotics”) in surface waters have been reported. As a possible input pathway in most cases municipal sewage has been discussed. But as an input from the realm of agriculture is conceivable as well, in this study it should be investigated if an input can occur via the pathway application of liquid manure on fields with the subsequent mechanisms surface run‐off/interflow, leaching, and drift. For this purpose a series of surface waters, soils, and liquid manures from North Rhine‐Westphalia (Northwestern Germany) were sampled and analyzed for up to 29 compounds by HPLC‐MS/MS. In each of the surface waters antibiotics could be detected. The highest concentrations were found in samples from spring (300 ng/L of erythromycin). Some of the substances detected (e.g., tylosin), as well as characteristics in the landscape suggest an input from agriculture in some particular cases. In the investigation of different liquid manure samples by a fast immunoassay method sulfadimidine could be detected in the range of 1…2 mg/kg. Soil that had been fertilized with this liquid manure showed a content of sulfadimidine extractable by accelerated solvent extraction (ASE) of 15 μg/kg dry weight even 7 months after the application. This indicates the high stability of some antibiotics in manure and soil.     

藻类对重金属的耐性与解毒机理

重金属是一类具有潜在危害的重要污染物,越来越多的重金属被排入水体,对水生生态环境构成严重威胁.藻类在长期响应重金属胁迫过程中,建立起一系列的适应机制.藻类通过控制重金属的吸收、富集、转运与解毒,使不同细胞组分中的重金属维持在正常浓度范围内.这些保护机制主要包括藻细胞的某些胞外组分与重金属结合,从而减少重金属进入胞内;在重金属诱导下藻细胞可合成金属结合蛋白或多肽;重金属诱导藻细胞合成一些代谢物使其免受伤害或修复由重金属胁迫造成的损伤;藻细胞通过液泡区室化作用使重金属远离代谢;藻细胞对重金属具有排斥与排出作用.     

Specific Features of Humic Substances Oxidation in Surface Waters in the Presence of Glucose

Experiments were conducted to study the microbial co-oxidation of naturally structured humic substances in different water bodies at different glucose concentrations. At low buffer capacity of aquatic ecosystems, the initial stages of the process are shown to be accompanied by a considerable increase in the medium acidity, a decrease in its alkalinity, and accumulation of colored intermediate products of bacterial and enzymatic synthesis. It is found that the co-metabolism processes with highly labile compounds either do not play any significant role in the trophodynamics of aquatic ecosystems (<10% of the original humic material is co-oxidized) or are not detected, notwithstanding the sufficient sensitivity of the analytical techniques involved. The low degree of co-oxidation (or its virtual absence) are found to be attributable to the masking effect of intermediate products.     

Heavy Metal Pollution in Sediments from Aquatic Ecosystems in China

We conducted an analysis of heavy metals content, including As, Cd, Cr, Cu, Hg, Pb, and Zn in sediments from aquatic ecosystems in China measured in recent publications. Then, we evaluated the extent of heavy metal pollution in these ecosystems in seven different industrial districts in China (Dongbei, Huabei, Huazhong, Huanan, Huaxik, Xibei, and Huadong) with the potential ecological risk index. We found that Cd was the most concentrated pollutant, followed by Hg and As, while Cr, Cu, Pb, Zn were found in low concentrations in sediments from all types of aquatic ecosystem in China. Sediments collected from all seven industrial districts were heavily polluted, and the sequence, from most to least polluted was Dongbei>Huabei>Huazhong>Huanan>Huaxi>Xibei>Huadong. All four types of aquatic ecosystem were found to be seriously polluted and the sequence, from most to least polluted was: river>sea>lake>wetland. Specifically, Cd and Hg were the most serious pollutants in all four aquatic ecosystems, and As was also a serious pollutant in rivers. For the seven industrial districts studied the sea was the most polluted ecosystem in Dongbei, the river was the most polluted ecosystem in Huabei, Huanan, Huazhong, and the lake was the most polluted in Huadong, Huaxi, and Xibei.     

西加拿大波利尔平原地表水富营养化管理技术

Surface waters in western Canada often experience eutrophication-related problems with water quality, specifically high internal phosphorus loading rates and excessive phytoplankton and macrophyte production, that lead to water column anoxia and restricted fisheries habitat. A group of researchers from across Canada were involved for a seven-year period in cooperative programs for surface water quality management in western Canada, based at the University of Alberta. This paper summarises information on two techniques, hypolimnetic oxygenation and lime (Ca₂CO₃ and Ca(OH)₂) application, used in western Canada (specifically, the province of Alberta) to manage eutrophication in standing waters (lakes and reservoirs).     

Expression of Mammalian Metallothionein-I Gene in Cyanobacteria to Enhance Heavy Metal Resistance

Metallothionein (MT) from mammals is a low molecular weight (about 6–7 KD), and cystein-rich (20 cystein per molecular) protein (Vallee, 1991). So MT has the strong binding ability of metal such as Cd, Hg and Pd. Wild-typed cyanobacteria have their own metallothionein-like proteins, which are of low cysteine content and have weak ability to bind heavy metal. Therefore, it is imperative to transfer the MT-I gene from mammal into cyanobacteria cell for the absorption and accumulation of heavy metals in aqueous system including marine and lake ( Misra and Gedamu, 1989). We inserted the MT-I cDNA gene after the strong promoter PpsbA or Psmt into the intermediary vector pRL-439, and then pRL-MT was ligated with shuttle vector pKT-210 (a shuttle vector pDC-08 was used at an earlier time) to construct the shuttle expression vector pKT-MT. (The MT-I mutant -KKS- also was selected as aim gene.) Then the constructed vector was introduced into cyanobacterium Anabaena sp. PCC7120, Synechocystis sp. PCC6803 and Synechococcus sp. PCC7942 with triparental conjunctive transfer (Bryant, 1994). The expression efficiency is about 1%. We also used homologous recombination vector pTZ18-8 to introduce MT-I cDNA into chromosomes in cyanobacteria and got higher expression efficiency. Metal tolerance tests show that the transgenic cyanobacteria acquire metal resistance up to 60 μmol/g CdCl₂ and expression efficiency up to 1045 μg MT/g fresh cells according to the data of ELISA.