The effects of UVR irradiance and spectral composition on yellow perch (<Emphasis Type="Italic">Perca flavescens</Emphasis>) larvae survival

The purpose of this study was to determine experimentally the effects of the quality (UV-A/UV-B ratio) and quantity (irradiance) of natural ultraviolet radiation (UVR) on the survival of yellow perch (Perca flavescens) larvae and on the oxidative stress in their cytoplasm, estimated by the activity of the superoxide dismutase (SOD). We also estimated the potential accumulation of photodamage in DNA by using UV dosimeters. Freshly-hatched yellow perch and UV dosimeters were incubated in controlled conditions under a factorial combination of selective and non-selective filters offering different levels of UVR protection and exposed to natural solar light. Larval survival was inversely related to the UVR intensity gradient, and responded similarly to the presence of both UV-A and UV-B or UV-A only. In contrast, the responses of SOD activity and UV dosimeter were stronger in the presence of both UV-A and UV-B than UV-A only, leading to a partial mismatch with the results on survival. Our results, obtained under natural solar light, suggest that incident UV-A radiation, despite its lower energy per unit photon than UV-B, could be a serious threat for yellow perch larvae. They also show that UV dosimeters and SOD activity predict only some components of UVR risk for yellow perch larvae. The partial mismatch between UV dosimeters results and larval survival cautions against the use of UV dosimeters alone as a proxy for UVR risk in the field.     

Effects of nutrients and irradiance on PSII variable fluorescence of lake phytoplankton assemblages

Phytoplankton from Lake Ontario and six small Canadian lakes (Dorset Lakes) were supplemented with nitrogen (N) and phosphorus (P) to determine how nutrients affect Photosystem II (PSII) variable fluorescence and photoinhibition in natural freshwater communities. Susceptibility of PSII to photoinhibition by photosynthetically active radiation (PAR) and ultraviolet radiation (UVR), as well as recovery potential, was quantified using changes in variable fluorescence and compared between N- and P-supplemented (Nu+) and non-supplemented (Nu?) Lake Ontario phytoplankton. Nu+ communities exhibited slightly higher variable fluorescence than Nu? when dark-adapted (F_v:F_m) or under constant illumination (F_q′:F_m′). Rates of relative electron transport (rETR) were greater for Nu+ than Nu? phytoplankton, with higher non-photochemical quenching (NPQ) by Nu? samples. The initial slope of the rETR-irradiance curve (α) did not differ significantly between nutrient treatments, but the saturation irradiance (E_K) was significantly higher for Nu+ samples than for Nu? samples. Nutrient supplementation increased rates of PAR- and UVR-dependent damage but also recovery, so that net PSII photoinhibition was equally severe as in the absence of added N and P. Additions of N, P, and N + P did not significantly alter F_v:F_m of Dorset Lakes phytoplankton. Compared to the range of variable fluorescence observable over the diel cycle of photoinhibition and recovery in Lake Ontario, the effects of supplemental nutrients observed in this study were minor.     

NH4+ enrichment and UV radiation interact to affect the photosynthesis and nitrogen uptake of Gracilaria lemaneiformis (Rhodophyta)

Solar ultraviolet radiation (UVR, 280-400 nm) is known to inhibit the photosynthesis of macroalgae, whereas nitrogen availability may alter the sensitivity of the algae to UVR. Here, we show that UV-B (280-315 nm) significantly reduced the net photosynthetic rate of Gracilaria lemaneiformis. This inhibition was alleviated by enrichment with ammonia, which also caused a decrease in dark respiration. The presence of both UV-A (315-400 nm) and UV-B stimulated the accumulation of UV-absorbing compounds. However, this stimulation was not affected by enrichment with ammonia. The content of phycoerythrin (PE) was increased by the enrichment of ammonia only in the absence of UVR. Ammonia uptake and the activity of nitrate reductase were repressed by UVR. However, exposure to UVR had an insignificant effect on the rate of nitrate uptake. In conclusion, increased PE content associated with ammonia enrichment played a protective role against UVR in this alga, and UVR differentially affected the uptake of nitrate and ammonia.     

Vibrational nitrogen concentration in the ionosphere and its dependence on season and solar cycle

A fully time-dependent mathematical model, SUPIM, of the Earth’s plasmasphere is used in this investigation. The model solves coupled time-dependent equations of continuity, momentum and energy balance for the O⁺, H⁺, He⁺, N⁺₂, O⁺₂, NO⁺ ions and electrons; in the present study, the geomagnetic field is represented by an axial-centred dipole. Calculation of vibrationally excited nitrogen molecules, which has been incorporated into the model, is presented here. The enhanced model is then used to investigate the behaviour of vibrationally excited nitrogen molecules with F10.7 and solar EUV flux, during summer, winter and equinox conditions. The presence of vibrational nitrogen causes a reduction in the electron content. The diurnal peak in electron content increases linearly up to a certain value of F10.7, and above this value increases at a lesser rate, in agreement with previous observations and modelling work. The value of F10.7 at which this change in gradient occurs is reduced by the presence of vibrational nitrogen. Vibrational nitrogen is most effective at F-region altitudes during summer daytime conditions when a reduction in the electron density is seen. A lesser effect is seen at equinox, and in winter the effect is negligible. The summer reduction in electron density due to vibrational nitrogen therefore reinforces the seasonal anomaly.     

青藏高原典型湖库光学吸收特性与光合有效辐射衰减系数初步研究

青藏高原地区分布的湖泊数量众多、面积较大、分布范围广泛.受制于恶劣的自然条件,对该地区湖泊的光学吸收特性以及光合有效辐射衰减系数(Kd(PAR))的研究鲜有成果.本文依据2014和2015年间采集的13个典型高海拔、湖泊面积较大的湖库的现场实测数据和实验室测定数据,分析了采样湖库各个采样点的Kd(PAR)特征以及有色可溶性有机物(CDOM)、藻类颗粒物吸收及非色素颗粒物吸收特性,计算并分析了Kd(PAR)与透明度以及光学活性物质的关系.研究结果表明:青藏高原地区各湖库平均各项颗粒物吸收系数均较低,总颗粒物吸收系数在400~700 nm波段内不超过0.14 m~(-1)、CDOM吸收系数在355 nm波长处最高,为1.23 m~(-1)、最低接近于0、藻类颗粒物吸收特性不明显;实验数据完整的巴木错、格仁错和班公错的主导吸收组分各异,其中巴木错为CDOM吸收主导,格仁错与班公错为非色素颗粒物吸收主导;青藏高原采样湖泊总体Kd(PAR)平均值较小,仅为0.26 m~(-1),样点最大值出现在可鲁克湖(1.17 m~(-1)),最小值出现在普莫雍错(0.10 m~(-1));在采样湖泊中Kd(PAR)与透明度呈显著相关;Kd(PAR)与CDOM的相关性最强,叶绿素a浓度次之,与总悬浮颗粒物浓度的相关性最不显著.     

抚仙湖秋、冬季光衰减特征及其与有色可溶性有机物的关系

为了研究抚仙湖紫外辐射(UVR)和光合有效辐射(PAR)衰减的时空特征及其与有色可溶性有机物(CDOM)、悬浮物(SS)、浮游植物(叶绿素a表征)等因子的关系,于2014年10月(秋季)、2015年1月(冬季)开展现场调查,结果显示:秋季不同波长(段)的漫射衰减系数Kd(305)、Kd(340)和Kd(PAR)分别为1.27±0.12、0.68±0.11和0.32±0.13 m-1,冬季分别为1.13±0.10、0.63±0.07和0.36±0.07 m-1;秋季CDOM的不同波长吸收系数ag(254)、ag(305)和ag(340)分别为4.09±0.26、1.18±0.09和0.57±0.05 m-1,冬季分别为2.95±0.24、0.61±0.11和0.11±0.07 m-1,秋季ag(254)、ag(305)和ag(340)显著高于冬季;秋季Kd(305)显著大于冬季,这与秋季(雨季)较高的CDOM丰度、浮游植物生物量(及SS浓度)有关.秋季ag(305)/Kd(305)、ag(340)/Kd(340)均显著高于冬季;秋季及秋冬季整体而言,ag(254)与Kd(305)、Kd(340)呈显著正相关,各多元逐步回归方程中均包含ag(254),说明CDOM吸收对UVR的衰减有重要贡献.空间差异方面,秋季北部的ag(254)、Kd(305)和Kd(340)显著高于南部,冬季南北部无明显差异,或与雨旱季北岸河流输入的CDOM和SS的情况有关.此外,浮游植物对UV-B衰减的影响和SS(与CDOM的交互作用)对UV-A衰减的影响更在于季节变化方面,而影响UVR、PAR衰减的各因子的相对贡献有待进一步量化.     

Comparison of the effective pressure law for permeability and resistivity formation factor in Chelmsford granite

Permeability, resistivity formation factor, and pore volume change were simultaneously measured on samples of Chelmsford granite subjected to confining pressure and pore pressure cycles. Using a technique described in a previous paper, the tangent coefficients of the effective pressure law for permeability _k and for formation factor _F were determined. _k and _F did not differ significantly from one another. They showed a strong stress history dependence as has already been observed for _k in several crystalline rocks. According to the definition of the effective pressure law used here, two physical properties with identical 's must be related through a one-to-one functional relationship. Hence, the observation above suggests that such a relationship may be empirically found between permeability and formation factor. Indeed, analysis of the data revealed that, to a good approximation, permeability was inversely proportional to the formation factor. The same relation has previously been observed in other crystalline rocks. This relationship was included in a recent version of the so-called equivalent channel model. Using this model, the specific surface area of the cracksA _c/V_S, the standard deviation of the distribution of asperities heightsh and the hydraulic radiusm _o were evaluated. The following values were respectively found: 850 cm^–1, 0.008 m and 0.14 m. The specific surface area of the cracks was independently estimated on micrographs of polished sections using a standard quantitative stereology method. The result was in good agreement with the values estimated from the transport properties data.     

Effect of stratospheric O3 depletion on tropospheric SO2 column in Antarctica

When ozone is depleted in the stratosphere during ozone-hole period, UV-B radiation, which is normally absorbed in this region, penetrates into the troposphere. As a result, the behavior of several species in the troposphere, which interact with UV-B radiation, is likely to change. Sulfur dioxide is predominantly found in the troposphere. To study the behavior of sulfur dioxide during this event, a Brewer spectrophotometer was installed at Maitri (70.7°S, 11.7°E) in Antarctica in July 1999. With this instrument, the vertical column density of SO₂ was measured from September 1999 to December 2003. We have observed an increase in SO₂ column density during an ozone-hole event. The magnitude of increase is different in different years, on average, by a factor of ∼5 (from ∼0.5 to ∼2.5 DU). Simultaneously, the maximum value of UV-B flux at the ground was also measured. We have observed that during the ozone-hole period, the UV-B flux also increased by different amounts during different years, by a factor of ∼3–5. Good correlation has been found between SO₂ column and UV-B flux but no correlation has been found between O₃ column and UV-B flux from the middle of September to the middle of November. Using a simple steady-state chemical reaction scheme, an attempt has been made to examine whether the increase in UV-B flux could increase the SO₂ column during the ozone-hole period.     

Die Bildung ionosphärischer Schichten bei beliebiger Temperaturverteilung in der Atmosphäre

Zusammenfassung Die Theorie der Schichtbildung durch eine monochromatische Strahlung wird für beliebige Temperaturverteilungen in der Atmosphäre erweitert. Unter gewissen Voraussetzungen ergeben sich durch Ionisation einer einzigen Komponente des Luftgemisches bereits zwei Maxima der Ionenbildung. Es wird vermutet, daß dieser Vorgang bei der häufig beobachteten Aufspaltung der F-Schicht in dieF ₁- undF ₂-Schicht vorliegt; aus der Theorie ergibt sich dann, daß die Temperatur in derF ₂-Schicht tiefer sein muß als in derF ₁-Schicht, weiters können verschiedene bisher bestandene Schwierigkeiten hinsichtlich derF ₂-Schicht behoben werden.

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Summary The ion-production of a monochromatic radiation in an atmosphere with variable temperature in various heights is considered. A stratification ofF-layer intoF ₁-andF ₂-layer appears by a heat zone between these layers. But always the temperature of theF ₂-layer is lower as this ofF ₁-layer. Thus some difficulties ofF ₂-layer can be removed.

     

Ecosystem water use efficiency in an irrigated cropland in the North China Plain

The eddy covariance technique and the cuvette method were used to investigate water use efficiency in an irrigated winter wheat (Triticum asetivum L.)/summer maize (Zea mays L.) rotation system in the North China Plain. The results show that ecosystem water use efficiency (WUE_e) changed diurnally and seasonally. Daily maximal WUE_e appeared in the morning. WUE_e generally peaked in late April in wheat field and in late July/early August in maize field. From 2003 to 2006, seasonal mean WUE_e was 6.7–7.4 mg CO₂ g⁻¹ H₂O for wheat and 8.4–12.1 mg CO₂ g⁻¹ H₂O for maize. WUE_e was much lower than canopy water use efficiency (WUE_c) under small leaf area index (LAI) but very close to WUE_c under large LAI. With the increase in LAI, WUE_e enlarged rapidly under low LAI but slowly when LAI was higher than one. WUE_e was greater on the cloudy days than on the sunny days. Under the same solar radiation, WUE_e was higher in the morning than in the afternoon. The ratio of internal to ambient CO₂ partial pressure (C_i/C_a) decreased significantly with the increase in photosynthetically active radiation (PAR) when PAR was lower than the critical values (around 500 and 1000 μmol m⁻² s⁻¹ for wheat and maize, respectively). Beyond critical PAR, C_i/C_a was approximately constant at 0.69 for wheat and 0.42 for maize. Therefore, when LAI and solar radiation was large enough, WUE_e has negative correlation with vapor pressure deficit in both of irrigated wheat and maize fields.     

人工模拟UV-B辐射对铜绿微囊藻(Microcystis aeruginosa)生长的影响

选择我国蓝藻水华的优势藻类铜绿微囊藻,进行人工模拟UV-B辐射连续24h照射实验,测定铜绿微囊藻生物量、生理及超微结构指标,探讨湖泊特别是高原湖泊藻类生长对UV-B辐射增强的胁迫响应.结果表明,当辐射时间达到1h时,铜绿微囊藻便开始陆续死亡,但蛋白质含量增加,且叶绿素a浓度保持稳定;当辐射时间少于4h时,细胞内超氧化物...     

Snow accumulation in forests from ground and remote‐sensing data

Winter‐forest processes affect global and local climates. The interception‐sublimation fraction (F) of snowfall in forests is a substantial part of the winter water budget (up to 40%). Climate, weather‐forecast and hydrological modellers incorporate increasingly realistic surface schemes into their models, and algorithms describing snow accumulation and snow‐interception sublimation are now finding their way into these schemes. Spatially variable data for calibration and verification of wintertime dynamics therefore are needed for such modelling schemes. The value of F was determined from snow courses in open and forested areas in Hokkaido, Japan. The value of F was related to species and canopy‐structure measures such as closure, sky‐view fraction (SVF) and leaf‐area index (LAI). Forest structure was deduced from fish‐eye photographs. The value of F showed a strong linear correlation to structure: F = 0·44 ? 0·6 × SVF for SVF < 0·72 and F = 0 for SVF > 0·72, and F = 0·11 LAI. These relationships seemed valid for evergreen conifers, larch trees, alder, birch and mixed deciduous stands. Forest snow accumulation (S_F) could be estimated from snowfall in open fields (S_o) and to LAI according to S_F = S_o (1 ? 0·11 LAI) as well as from SVF according to S_F = S_o (0·56 + 0·6 SVF) for SVF < 0·72. The value of S_F was equal to S_o for SVF values above 0·72. The value of sky‐view fraction was correlated to the normalized difference snow index (NDSI) using a Landsat‐TM image for observation plots exceeding 1 ha. Variables F and S_F were related to NDSI for these plots according to: F = ?0·37NDSI + 0·29 and S_F = S_o (0·81 + 0·37NDSI). These relationships are somewhat hypothetical because plot‐size limitation only allowed one sparse‐forest observation of NDSI to be used. There is, therefore, a need to confirm these relationships with further studies. Copyright © 2004 John Wiley & Sons, Ltd.     

A role of the altitude gradient of the thermospheric wind velocity in ionospheric <Emphasis Type="Italic">F</Emphasis>-region dynamics

By processing the data of vertical ionospheric sounding in Almaty for 2000–2009, we obtained the distributions of the heights of the maximum (h _m F) and bottom (h _bot F) of the F2-layer, incremental changes in its semi-thickness (δh), the characteristic time of losses (τ), and the vertical displacement velocity of the node of the thermospheric wind (V) during the transitional time of the day during nighttime increases in the electron concentration at the layer maximum. The comparison of the measured V and modeled V _m velocities showed a certain discrepancy. The influence of the altitude gradient of the meridional thermospheric wind velocity on the behaviors of h _m F, h _bot F, δh, and τ during nighttime increases in the electron concentration is studied.     

Improved method to determine the molar volume and compositions of the NaCl-H<Subscript>2</Subscript>O-CO<Subscript>2</Subscript> system inclusion

On the basis of Parry’s method (1986), an improved method was established to determine the molar volume (Vm) and compositions (X) of the NaCl-H2O-CO2 (NHC) system inclusion. To use this method, the determination of Vm-X only requires three microthermometric data of a NHC inclusion: partial homog-enization temperature (Th ,CO2), salinity (S) and total homogenization temperature (Th). Theoretically, four associated equations are needed containing four unknown parameters: X CO2, XNaCl, Vm and F (volume fraction of CO2 phase in total inclusion when occurring partial homogenization). When they are released, the Vm-X are determined. The former three equations, only correlated with Th ,CO2, S and F, have simplified expressions:XCO2=f1(Th,CO2,S,F),XNaCl=f2(Th,CO2,S,F),Vm=f3(Th,CO2,S,F). The last one is the thermodynamic relationship of X CO2, XNaCl, Vm and Th:f4(XCO2,XNaCl,Vm,Th)=0.Since the above four associated equations are complicated, it is necessary to adopt iterative technique to release them. The technique can be described by:(i) Freely input a F value (0≤F≤1),with Th ,CO2 and S, into the former three equations. As a result,X CO 2,XNaCl and the molar volume value recorded as Vm1 are derived. (ii) Input the X CO2 and XNaCl gotten in the step above into the last equation, and another molar volume value recorded as Vm2 is determined. (iii) If Vm1 is unequal to Vm2, the calculation will be restarted from “(i)”. The iteration is completed until Vm1 is equal to Vm2, which means that the four associated equations are released. Compared to Parry’s (1986) solution method, the improved method is more convenient to use, as well as more accurate to determine X CO 2. It is available for a NHC inlusion whose partial homogenization temperature is higher than clatherate melting temperature and there are no solid salt crystals in the inclusion at parital homogenization.     

Implications of ozone depletion for surface-water photochemistry: Sensitivity of clear lakes

Increased ultraviolet-B radiation (UV-B) in aquatic ecosystems brought about by stratospheric ozone depletion may result in increased formation rates of photochemical reaction products in the surface waters of aquatic ecosystems. In this study, the potential impact of increases in lower wave-length radiation on the formation of hydrogen peroxide (H₂O₂) and singlet oxygen (¹O₂) was modelled for lakes over a range of dissolved organic carbon (DOC) concentrations. The impact of increased UV-B radiation on the production of carbon monoxide (CO) was also calculated for two humic stained systems. The relative increases of H₂O₂ and¹O₂ production were greater in the clear lakes (70% increase in photochemical reaction rates near the surface) than the coloured systems (25%). Production of CO in the study sites also increased under depleted ozone conditions implying increased DOC losses to the overlying atmosphere.