New Attempt to Reduce the Harm of Smoking: Reducing the Nitrosamines Level in Tobacco Smoke by Microwave Irradiation

In order to protect the environment and public health, microwave irradiation was investigated as a means of reducing the nitrosamines level of tobacco smoke under mild conditions where the microwave energy was 1 kW and the irradiation time was shorter than 2 min. The microwave‐induced elimination of nitrosamines such as N‐nitrosopyrrolidine (NPYR) and N‐nitrosodiphenylamine (NDPA) was investigated and the impact of the presence of water and salt in the medium on the reduction was assessed in detail. The existence of water in the medium was crucial for the microwave‐induced reduction of NDPA, because the rapid movement of water molecules under the influence of microwaves strongly affects the adjacent nitrosamines leading to their decomposition. This was the first time that microwaves have been used to directly irradiate cigarettes sealed in their packaging, and the nitrosamines content of tobacco and smoke of these irradiated cigarettes were analyzed with care. Irradiation for 90 s with microwave energy of 1kW was seen to reduce 25 to 30% of the nitrosamines in tobacco and 50 to 60% of the levels present in the mainstream smoke of the cigarette. Moreover, lower mutagenic activity and higher CHO cell livability were found for the irradiated cigarettes in comparison with the control according to the results of in vitro tests.     

Effects of smoking on the levels of urinary biomarkers of aromatic hydrocarbons in oil refinery workers

Occupational exposures to aromatic hydrocarbons may be overwhelmed by mainstream and secondary smoking exposures. The objective of this study was to evaluate the influence of occupational exposures and smoking on benzene and toluene urinary metabolites. The metabolites were measured in pre and post-work shift urine specimens in oil refinery workers by gas chromatography/mass spectrometry techniques. Post-shift concentration differences between non-smokers and smokers were statistically significant (at p-value?<?0.05). Pre- and post-shift concentrations of urinary phenol (benzene biomarker) did not vary significantly for both non-smokers and smokers. Occupational exposures to toluene triggered an increase of post-shift levels (compared to pre-shift levels) of urinary cresol for non-smokers and workers who had less than 10 cigarettes during the work shift. For these groups, non-occupational exposures before and during the work shift did not vary. For smokers who did not smoke during the work shift and for those who had more than ten cigarettes during the shift, the post-shift levels of both cresol and hippuric acid were reduced approximately 30% lower than pre-shift levels. This is due to increased exposures to tobacco smoke before the work shift. The relationships between phenol and (cresol?+?hippuric acid) levels for non-smokers and smokers indicated that elevated co-exposures due to smoking result in the reduction of all metabolites levels in urine. These findings demonstrated that exposures to tobacco smoke may stochastically interfere with occupational exposures when biological monitoring is used to assess occupational health risks. Factors influencing the magnitude of the interference were specimen collection time (in relation to the timing of occupational exposures and excretion rates of biomarkers), smoking intensity and timing before and during the work shift.     

Direct interpretation of 2D potential fields for deep structures by means of the quasi-singular points method

The founder of the Russian school of direct interpretation of potential fields (with minimal prior geological‐geophysical information) was V.M. Berezkin, who introduced the operator of total normalized gradient for the 2D interpretation of profile gravity data sets. This operator was successfully applied in searches of hydrocarbon reservoirs. The further development of this approach (the so‐called quasi‐singular points method) has allowed solution also to various structural problems, using mathematical criteria for the transition from extremes of total normalized gradient fields to coordinates of anomalous sources. The main numerical evaluation strategy is based on stabilized downward continuation of field derivatives and specific use of the filtration properties of Fourier series approximation. The characteristic properties of the quasi‐singular points method are: 1) presentation of a more general total normalized gradient function through additional parameters (derivative order m, form of smoothing function Q, number of Fourier coefficients N* with maximal N), optimum values being chosen during a peak‐spectrum analysis of the interpreted function; 2) calculation of the set of total normalized gradient fields for various values of N*/N, representing coordinate systems {x,N*/N} as an ‘axes tree’ of extrema, where each 2D total normalized gradient field is representationally compressed in a 1D line, permitting a) immediate overview of the positions of the axes in all variants of the calculated fields and b) reduction of the retained information, as required in subsequent interpretation; 3) development of two criteria for transition from extrema of total normalized gradient fields to the coordinates of anomaly sources. The quasi‐singular points method is intended for tracing limiting gently‐sloping boundaries, if their micro‐relief features are sources of the interpreted anomaly but sub‐vertical contacts may also be traced. The method has been tested in delineating various geological structures. One of the most challenging, successfully achieved, was tracing of the Moho discontinuity and study of the upper mantle, using only Bouguer anomaly data along interpretation profiles. This is attested in an example of two regional profiles intersecting the European part of Russia. The central part of one of them coincides with the results from a deep seismic profile.     

Robust integrated actuator control: experimental verification and real‐time hybrid‐simulation implementation

In this paper, we propose a new actuator control algorithm that achieves the design flexibility, robustness, and tracking accuracy to give real‐time hybrid‐simulation users the power to achieve highly accurate and robust actuator control. The robust integrated actuator control (RIAC) strategy integrates three key control components: loop shaping feedback control based on H_∞ optimization, a linear‐quadratic‐estimation block for minimizing noise effect, and a feed‐forward block that reduces small residual delay/lag. The combination of these components provides flexible controller design to accommodate setup limits while preserving the stability of the H_∞ algorithm. The efficacy of the proposed strategy is demonstrated through two illustrative case studies: one using large capacity but relatively slow actuator of 2500 kN and the second using a small‐scale fast actuator. Actuator tracking results in both cases demonstrate that the RIAC algorithm is effective and applicable for different setups. Real‐time hybrid‐simulation validation is implemented using a three‐DOF building frame equipped with a magneto‐rheological damper on both setups. Results using the two very different physical setups illustrate that RIAC is efficient and accurate. Copyright © 2014 John Wiley & Sons, Ltd.     

In situ characterization of grain‐scale fluvial morphology using Terrestrial Laser Scanning

The grain‐scale morphology of fluvial sediments is an important control on the character and dynamics of river systems; however current understanding of its role is limited by the difficulties of robustly quantifying field surface morphology. Terrestrial Laser Scanning (TLS) offers a new methodology for the rapid acquisition of high‐resolution and high‐precision surface elevation data from in situ sediments. To date, most environmental and fluvial applications of TLS have focused on large‐scale systems, capturing macroscale morphologies. Application of this new technology at scales necessary to characterize the complexity of grain‐scale fluvial sediments therefore requires a robust assessment of the quality and sources of errors in close‐range TLS data. This paper describes both laboratory and field experiments designed to evaluate close‐range TLS for sedimentological applications and to develop protocols for data acquisition. In the former, controlled experiments comprising high‐resolution scans of white, grey and black planes and a sphere were used to quantify the magnitude and source of three‐dimensional (3D) point errors resulting from a combination of surface geometry, reflectivity effects and inherent instrument precision. Subsequently, a methodology for the collection and processing of grain‐scale TLS data is described through an application to a coarse grained gravel system, the River Feshie (D₅₀ 32 to 63 mm). This stepwise strategy incorporates averaging repeat scans and filtering scan artefact and non‐surface points using local 3D search algorithms. The sensitivity of the results to the filter parameter values are assessed by careful internal validation of Digital Terrain Models (DTMs) created from the resulting point cloud data. The transferability of this methodology is assessed through application to a second river, Bury Green Brook, dominated by finer gravel (D₅₀ 18 to 33 mm). The factor limiting the resolution of DTMs created from this second dataset was found to be the relative sizes of the laser footprint and smallest grains. Copyright © 2009 John Wiley & Sons, Ltd.     

Separation and Preconcentration of Cobalt Using a New Schiff Base Derivative on Amberlite XAD‐7

In this study, a new solid‐phase extraction procedure has been developed for preconcentration and determination of Co ions in different water samples by flame atomic absorption spectrometry (FAAS). Cobalt was preconcentrated as N,N′‐bis(pyridine‐2‐yl‐methyl)benzene‐1,4‐diamine (Co‐BPMBDA) from sample solutions using a column containing Amberlite XAD‐7 and was determined. In order to achieve the best performance for the method, effects of several parameters such as pH, concentrations of ligand, sample flow rate, eluent, and matrix ions on the method efficiency were investigated. Under optimum conditions, the preconcentration factor was found to be 200 for 1000 mL waters samples. Detection limit based on the 3S_b criterion was calculated as 0.24 µg/L for 100 mL of sample solution and relative standard deviation was found to be 1.8%. The method was applied to determine the trace amounts of cobalt in water samples.     

Cadmium‐Induced Physiological Response in Lonicera japonica Thunb.

Phytoremediation of Cd‐contaminated soil using hyperaccumulators has become a new promising technique. Lonicera japonica Thunb. has been reported as a new Cd‐hyperaccumulator. In this study, the effect of Cd stress duration on growth, photosynthesis and mineral nutrition of L. japonica was investigated. At 30 days after Cd stress, there was not any visual leaf symptoms in L. japonica, and an obvious stimulating effect of 10 mg kg^?1 Cd on net photosynthesis rate (P_N) was well correlated to photosynthetic pigment contents and mineral nutrition (Mg and Fe) concentrations. With Cd stress time extended, no significant differences of shoots and total biomass and P_N compared with the control was observed, indicating that L. japonica could develop effective tolerance mechanisms to avoid Cd‐induced damage to photosynthesis and growth. The photosynthetic performance remained functional through stomatal and non‐stomatal adjustments, and mineral nutrition responses. The improved growth based on shoots and total biomass and P_N by 10 mg kg^?1 Cd, as suggested by hormesis, may be beneficial to enhance the potential for phytoremediation, because it typically faced the low Cd concentrations in actual Cd‐contaminated soils. The study results indicated that L. japonica could be used for phytoremediation contaminated soils by Cd.     

Monitoring of Microbiological Attributes of Soil and Groundwater Exposed to Metal Industrial Effluents

The present paper evaluates the temporal and spatial impact of heavy metal containing (Cr, Zn, Cu, Ni, Cd and Fe) effluents of brass, electroplating and tannery industries on chemical and microbiological characteristics of affected soil and groundwater. Therefore, samples were drawn from three sites, S₁, S₂ and S₃, with a longitudinally distant from effluent drain of 20, 200 and 700 m, respectively. In general, the metals concentration exceeded the standard limits not only in the discharged effluents but also in the soil and groundwater. The significant reduction of microbial biomass C and N, soil respiration and microbial coefficient with increasing metal content from S₃ to S₁ was recorded, the effect being more pronounced in summer. However, the C_mic/N_mic ratio decreased whilst the metabolic quotient ($q_{{\rm CO}_{{\rm 2}} } $ ) increased with increasing metal concentration in soil. The content of Zn (11.5 mg/L) and Bacillus sp. was at maximum in groundwater of brass and electroplating industry site, whereas that of tannery site contained maximum Cr (2.34 mg/L) and Enterobacter sp. The toxic metals adversely polluted the groundwater which made it to harbours Escherichia coli beyond the prescribed limit. To check the soil and groundwater pollution, eco‐friendly measures involving improved effluent treatment technology and site‐specific application of treated effluent are recommended.     

Optimization and Modeling of the Photocatalytic Degradation of the Insect Repellent DEET in Aqueous TiO2 Suspensions

Response surface methodology (RSM) and artificial neural networks (ANNs) based on a multivariate central composite design (CCD) were applied to model and optimize the photocatalytic degradation of N,N‐diethyl‐m‐toluamide (DEET). The individual and interaction effects of three main operating factors (mass of TiO₂, initial DEET concentration, and irradiation intensity) on process efficiency were estimated, proving their important effect on % DEET removal. Among the independent variables, TiO₂ concentration displayed the highest effect on DEET degradation followed by initial DEET concentration and UV intensity. The optimization and prediction capabilities of ANNs and RSM were compared on the basis of root mean squared error, mean absolute error, absolute average deviation, and correlation coefficient values. Results proved the usefulness and capability of the experimental design strategy for successful investigation and modeling of the photocatalytic process. Moreover, the selected ANN gave better estimation capabilities throughout the range of variables than RSM. Based on the models and the related experimental conditions, the optimal values of each parameter were determined. Under optimum conditions, DEET and total organic carbon (TOC) followed pseudo‐first order kinetics. Nearly complete degradation of DEET took place within 15 min whereas high TOC removal percentages (>85%) was achieved after 90 min irradiation time.     

Transformation of Tobacco with ScMTII Gene‐Enhanced Cadmium and Zinc Accumulation

Genetic transformation is gaining importance for developing plant types suitable to metal accumulate and/or hyperaccumulate. In this study, the transgenic tobacco plant which transferred the ScMTII gene from Saccharomyces cerevisiae to wild type tobacco cultivar Petite Havana (SR1) was grown on soils with low and high cadmium (Cd) and zinc (Zn) concentrations in a growth chamber for 6 weeks and compared to wild type tobacco for Cd and Zn accumulation. Cadmium and Zn accumulations in the transgenic and wild type tobacco plants were increased with the increasing Cd and Zn concentrations. Unlike Zn, the transgenic plant accumulated significantly higher amount of Cd compared to the wild type control plants. Shoot Cd concentrations of transgenic tobacco in higher Cd dosages reached the above the hyperaccumulation threshold value of 100 mg Cd kg^?1 in the dry weight (DW). Transgenic tobacco accumulated 354, 400, 372, and 457 mg Cd kg^?1 DW, for 10, 20, 40, and 80 mg Cd kg^?1 soil treatments, respectively. These values are 3.5–4.5‐fold higher than that of Cd hyperaccumulation threshold value. With 10 mg kg^?1 Cd treatment, the bioconcentration factor (BCF) of transgenic tobacco plants for Cd reached up to 35 in which the threshold value for BCF should be at least 10. Our results showed that the transgenic tobacco may be used as a good Cd hyperaccumulator plant and for phytoextraction of Cd contaminated soils, but not for Zn.     

Influence of Vegetation Characteristics on Soil Denitrification in Shoreline Wetlands of the Danjiangkou Reservoir in China

Soil denitrification in reservoir shoreline wetlands is an important process for removing excess inorganic nitrogen from upland runoff and controlling eutrophication in aquatic ecosystems. As yet, little is known about the influence of vegetation characteristics on the soil denitrification potential in reservoir shoreline wetlands, although vegetation can affect both denitrifying bacteria and soil properties. In this study, we measured the spatial variability of denitrification enzyme activity (DEA) using acetylene block method in shoreline wetlands of the Danjiangkou Reservoir, a water source of the South‐to‐North Water Transfer Project in China. Results indicated that DEA ranged from 0.001 to 2.449 µg N (N₂O) g^?1 h^?1, with a mean of 0.384 µg N (N₂O) g^?1 h^?1. DEA varied significantly among five representative plant communities and the highest DEA (0.248–2.449 µg N (N₂O) g^?1 h^?1) was observed in the Polygonum hydropiper community. Plant biomass and vegetation cover were significantly and positively related to DEA and together explained 44.2% of the total variance. These results suggest that vegetation characteristics should also be considered in assessing soil denitrification capacity and restoring shoreline wetlands for nitrogen pollution removal in the Danjiangkou Reservoir after dam heightening.     

Earthquake response spectra taking account of number of response cycles

Proposed is a new definition of earthquake response spectra, which takes account of the number of response cycles N. The Nth largest amplitude of absolute acceleration response of a linear oscilator with natural period T and damping ratio h, which is subjected to ground motion at its base, is defined as S_A(T, h, N). By defining a reduction factor η(T, h, N) as S_A(T, h, N)/S_A(T, h, 1), characteristics of η(T, h, N) were investigated based on 394 components of strong motion records obtained in Japan. Two practical empirical formulae to assess the reduction factor η(T, h, N) are proposed.     

The Effects of Different Substrates on Ammonium Removal in Constructed Wetlands: A Comparison of Their Physicochemical Characteristics and Ammonium‐Oxidizing Prokaryotic Communities

A vertical flow constructed wetland (VFCW) filled with natural zeolite had higher ammonium removal (82.4–94.0%) than another VFCW filled with volcanic rock (51.3–69.4%) treating piggery wastewater under any of three recirculation ratios. To reveal the underling reasons, physicochemical characteristics and ammonium‐oxidizing prokaryotes (AOPs) of two substrates were determined. The calculated maximum ammonium adsorption of zeolite (11.6 mg g^?1) was remarkably higher than that of volcanic rock (0.21 mg g^?1), mainly because the former contains two cationic binding crystalline species, and montmorillonite. Quantitative analyses indicated that ammonium‐oxidizing archaea (AOA) are the dominant AOP of zeolite and ammonium‐oxidizing bacteria (AOB) are the dominant AOP of volcanic rock respectively. Diversity and phylogenetic analysis further showed that both AOB and AOA communities were significantly different between two substrates. Nitrosospira‐like AOB (92.6%) and sediment/soil clade AOA (71.4%) were the main components of AOB and AOA in natural zeolite, whereas Nitrosomonas‐like AOB (76.1%) and water/sediment clade AOA (77.1%) dominated the AOB and AOA in volcanic rock. These results suggest that the substrates show high selectivity to AOP community. Thus, in addition to physicochemical characteristics, the AOP of substrates is very likely to affect ammonium removal in VFCWs.     

Significance of an In Vivo Genotoxicity Test for Surface Waters Is Increased when the Physiological Characteristics of the Test Organism (Bivalvia: Mollusca) Are Taken into Account?

The alkaline filter elution assay using the gills of the freshwater clam species Corbicula fluminea detects breaks in single‐stranded DNA and is thus a good method for determining the genotoxic potential of surface waters. In attempting to standardize the procedure, a wide range of factors which could have an influence on the uptake of genotoxic substances by the exposed clams were studied. The most important parameters of the static exposure in relation to the rate of filtration by the animals turned out to be the temperature, the volume of the water, and the exposure time. Differences in body size and in the amount of suspended particles in surface waters did not play a significant role. The results demonstrate that the in vivo test system can be quite sensitive and its results reproducible when the relevant species‐specific characteristics of the test organisms are brought into consideration, even if the test organism belongs to a biologically more advanced group. A clear dose‐response relationship to the reference substances 4‐nitroquinoline‐1‐oxide (NQO) and N‐methyl‐N‐nitro‐N‐nitrosoguanidine (MNNG) can be observed even after a short exposure time of between four and twenty hours. No genotoxic effects were observed when using this method on surface waters from the area of Cologne (including water from the Rhine River and within the protection zone 2 of the Cologne waterworks).     

Review Paper: A semi‐empirical model of strain sensitivity for 4D seismic interpretation

We reformulate the original model of Hatchell and Bourne and Røste, Stovas and Landrø that couples fractional velocity change to subsurface strain via a fundamental constant R. The new model combines elastic compressibility of a dual‐porosity system for a sand–shale mixture with horizontal planes of inter‐granular weakness. The majority of observed R‐factor magnitudes from post‐stack 4D seismic data in both the reservoir and overburden can thus be explained. R is predicted to depend strongly on lithology and also initial strain state. The model is also extended to predict the observed angle‐dependence of time‐lapse time‐shifts from pre‐stack data. An expression for the gradient of time‐shift with incidence angle is obtained in terms of the background V_P/V_S, and also the ratio of tangential to normal compliances B_T/B_N representing loss or creation of inter‐granular coupling. If accurately estimated from data, this compliance ratio can be used as an additional parameter to assess the post‐production state of the overburden. It is concluded that whilst R remains the over‐arching parameter controlling the magnitude of time‐shifts measured from 4D seismic data, B_T/B_N is a subtler parameter that may also prove of future value.     

Hybrid seismic protection of cable‐stayed bridges

This paper proposes a hybrid control strategy combining passive and semi‐active control systems for seismic protection of cable‐stayed bridges. The efficacy of this control strategy is verified by examining the ASCE first‐generation benchmark problem for a seismically excited cable‐stayed bridge, which employs a three‐dimensional linearized evaluation bridge model as a testbed structure. Herein, conventional lead–rubber bearings are introduced as base isolation devices, and semi‐active dampers (e.g., variable orifice damper, controllable fluid damper, etc.) are considered as supplemental damping devices. For the semi‐active dampers, a clipped‐optimal control algorithm, shown to perform well in previous studies involving controllable dampers, is considered. Because the semi‐active damper is a controllable energy‐dissipation device that cannot add mechanical energy to the structural system, the proposed hybrid control strategy is fail‐safe in that the bounded‐input, bounded‐output stability of the controlled structure is guaranteed. Numerical simulation results show that the performance of the proposed hybrid control strategy is quite effective in protecting seismically excited cable‐stayed bridges. Copyright © 2004 John Wiley & Sons, Ltd.     

River discharge,land use change,and surface water quality in the Xiangjiang River,China

To compare the impacts of river discharge on the surface water quality of the Xiangjiang River in China, 12 surface water quality parameters recorded at 31 sampling sites from January 1998 to December 2008 along the river and its main tributaries were analyzed. Significantly higher concentrations of total nitrogen, ammoniacal nitrogen, and total phosphorus, and biochemical oxygen demand were observed during low‐flow periods than during high‐flow periods, implying a higher risk to local residents drinking untreated water during low‐flow periods. Pollution indexes, including the inorganic pollution index and integrated pollution index (IPI), were negatively related to impervious surface area (ISA) and cropland area (CLA) when ISA (CLA) was less than 160 (3000) km². However, the relationship was positive when ISA (CLA) was larger than 160 (3000) km², which provided a reasonable explanation for the observed spatial patterns of water quality. Distinct increasing temporal trends for two kinds of pollution indexes were also found. The annual ISA was significantly related to the rapid degradation of water quality from 1998 to 2008, with correlation coefficient (r) values of 0.816 (p = 0.002) and 0.711 (p = 0.014) for the organic pollution index (OPI) and IPI, respectively. However, annual rainfall was negatively correlated with the two indexes with r values of 0.785 (p = 0.002) and 0.448 (p = 0.093) for OPI and IPI, respectively. Our study highlights that decision makers should be more aware of recent increases in the pollution of the Xiangjiang River, especially at downriver sites and during low‐flow periods. Copyright © 2013 John Wiley & Sons, Ltd.     

Fluoride Biomonitoring around a Large Aluminium Smelter Using Foliage from Different Tree Species

In order to study the pollution gradient in the vicinity of a large aluminium production facility in Patagonia (Argentina), a passive biomonitoring was performed employing foliage from three tree species. Primary scope was to identify pollution gradients and to select suitable tree species which can be used as biomonitor plants in the study area. Therefore, leaves of Eucalyptus rostrata, Populus hybridus and different needle ages of Pinus radiata were collected at different distances from the industry and the fluoride concentration was analysed in washed and unwashed samples in order to determine the amount of external fluoride. Washing reduced the F‐concentrations by 24, 39 and 51% on average in E. rostrata, P. hybridus and P. radiata, respectively, indicating that species‐specific characteristics determine the accumulation and wash‐off of dust‐associated fluorine. F‐concentrations varied from 6 to 3652 ppm F in unwashed samples indicating a steep pollution gradient in the study area. The influence of F‐emissions was discernible in all samples up to a distance of 3500 m from the smelter. E. rostrata accumulated more fluorine than the other species at equal distance from the emission source. The present study confirms that aluminium smelting results in high F deposition in the study area. Establishing a biomonitor network around large emitters is suitable and feasible to evaluate the efficiency of air control measures.     

Geographies of uncertainty in the health benefits of air quality improvements

Assessing the long-term benefits of marginal improvements in air quality from regulatory intervention is methodologically challenging. In this study, we explore how the relative risks (RRs) of mortality from air pollution exposure change over time and whether patterns in the RRs can be attributed to air quality improvements. We employed two-stage multilevel Cox models to describe the association between air pollution and mortality for 51 cities with data from the American Cancer Society (ACS) cohort (N = 264,299, deaths = 69,819). New pollution data were computed through models that predict yearly average fine particle (PM_2.5) concentrations throughout the follow-up (1982–2000). Average PM_2.5 concentrations from 1999 to 2000 and sulfate concentrations from 1980 were also examined. We estimated the RRs of mortality associated with air pollution separately for five time periods (1982–1986, 1987–1990, 1991–1994, 1995–1998, and 1999–2000). Mobility models were implemented with a sub-sample of 100,557 subjects to assist with interpreting the RR estimates. Sulfate RRs exhibit a large decline from the 1980s to the 1990s. In contrast, PM_2.5 RRs follow the opposite pattern, with larger RRs later in the 1990s. The reduction in sulfate RR may have resulted from air quality improvements that occurred through the 1980s and 1990s in response to the acid rain control program. PM_2.5 concentrations also declined in many places, but toxic mobile sources are now the largest contributors to PM in urban areas. This may account for the heightened RR of mortality associated with PM_2.5 in the 1990s. The paper concludes with a three alternative explanations for the temporal pattern of RRs, each emphasizing the uncertainty in ascribing health benefits to air quality improvements.     

A Preliminary Survey of the Level of Microbiological Pollution of Major Rivers in Northern Greece

The objective of the present survey was to assess the microbiological quality of river waters used mainly to irrigate the large plains of Macedonia and Thessaly in Northern Greece. Five rivers (Aliakmonas, Axios, Loudias, Mavroneri and Pineios) were sampled during a 10‐month period (June 2002 – March 2003) for Total Microbial Flora (TMF) at 22 °C and 37 °C, Total Coliforms (TC), Fecal Coliforms (FC), enterococci, staphylococci, Pseudomonas, fungi, Giardia spp. and Cryptosporidium spp. The results indicate moderate to high microbiological pollution, with the highest levels of microbial pollution found in Mavroneri and Pineios Rivers, and moderate pollution levels in Aliakmonas, Axios and Loudias Rivers. The extent of microbiological pollution is accentuated by the fact that Giardia‐ and/or Cryptosporidium cysts were detected in nine out of eleven samples collected in June 2002 with excessive Giardia counts demonstrated for Pineios River. The microbiological pollution of the surveyed rivers is attributed to various degrees of human interference and to lack of ample pollution monitoring systems, which emphasize the need for implementation of the EU Water Framework Directive.