Hebei Spirit oil spill monitored on site by fluorometric detection of residual oil in coastal waters off Taean, Korea

The spatiotemporal distributions of dissolved and/or dispersed oil in seawater and pore water were monitored on site by fluorometric detection method after the Hebei Spirit oil spill. The oil concentrations in intertidal seawater, 15 days after the spill, were as high as 16,600 μg/L and appeared to decrease below the Korean marine water quality standard of 10 μg/L at most sites 10 months after the spill. Fluorometric detection of oil in pore water was introduced to eliminate the effects of grain size for the quantification of oil in sediments and to better explain spatial and temporal distribution of oil pollution at sandy beaches. The fluorescence detection method was compared with the conventional laboratory technique of total petroleum hydrocarbon analysis using gas chromatography. The method of fluorescence detection of oil was capable of generating results much faster and more cost-effectively than the traditional GC technique.     

Simulating the influence of physicochemical parameters on subsurface oil on beaches: Preliminary results

Monitoring of sandy beaches after the Prestige oil spill revealed thick subsurface layers (up to 1 m thick) of grey-coloured sand. These horizons were sometimes found under more than 3 m of clean sand. Examination of the sand by electron microscopy confirmed that the colouring was due to oil-coated sand grains, and revealed a sequence of degradation of buried oil. Further analysis of the sand revealed high concentrations of hydrocarbon in the oil-coated sand and that the main biomarkers were indicative of biodegradation, even though the oil was buried.A set of experiments was designed to analyze the evolution of oil from tar balls to coatings. The results revealed that biodegradation is a secondary process in the changes that take place in the buried oil, and that water flow slows down the appearance of grey sand and that low salinity may hinder the oil degradation process.     

A laboratory study on the kinetics of the formation of oil-suspended particulate matter aggregates using the NIST-1941b sediment

The formation of oil-suspended particulate matter aggregates (OSAs) results from the heteroaggregation between dispersed oil droplets and suspended particulate matter present in coastal waters. This process has been recognized by the oil spill remediation community to enhance natural cleansing of oiled shorelines and oil dispersion in the water column. While several studies have been conducted on the formation and characteristics of OSAs, few studies have addressed the kinetics of OSA formation. Operationally, this has left decision-makers lacking information on the time scale of this process and its significance to oil dispersion in real spills. A laboratory study was conducted to investigate the kinetics of OSA formation as a function of mixing energy and the sediment-to-oil ratio using the standard reference material 1941b. Results showed that formation of OSAs increased exponentially with the mixing time and reached a maximum within 4 h. When the shaking rate increased from 2.0 to 2.3 Hz, the maximum oil trapping efficiency increased from 20% to 42% and the required shaking time decreased from 3.7 to 0.7 h.     

Effects of chronic exposure to dispersed oil on selected reproductive processes in adult blue mussels (Mytilus edulis) and the consequences for the early life stages of their larvae

Mussels (Mytilus edulis) were continuously exposed to dispersed crude oil (0.015-0.25 mg/l) for 7 months covering the whole gamete development cycle. After 1 month exposure to 0.25 mg oil/l, the level of alkali-labile phosphates (ALP) and the volume density of atretic oocytes in female gonads were higher than those in the gonads of control females, indicating that oil affected the level of vitellogenin-like proteins and gamete development. Spawning of mussels was induced after 7 months oil exposure. Parental oil exposure did not affect subsequent fertilization success in clean seawater but this was reduced in 0.25 mg oil/l. Parental exposure to 0.25 mg oil/l caused both slow development and a higher percentage of abnormalities in D-shell larvae 2 days post-fertilization; reduced growth 7 days post-fertilization. These effects were greatly enhanced when larval stages were maintained at 0.25 mg oil/l. Similar studies are warranted for risk assessment prognosis.     

Phase distribution of hydrocarbons in the water column after a pelagic deep ocean oil spill

Spills from wrecks are a potential major source of pollution in the deep ocean. However, not much is known about the fate of a spill at several kilometers depth, beyond the oceans continental shelves. Here, we report the phase distribution of hydrocarbons released from the wrecks of the Prestige tanker, several years after it sank in November 2002 to depths between 3500 and 3800 m. The released oil reached the surface waters above the wrecks without any signs of weathering and leaving an homogenous signature throughout the water column. At depths of several kilometers below the sea surface, the occurrence and spread of the deep sea oil spill could be evaluated better by quantifying and characterizing the dissolved hydrocarbon signature, rather than just the investigation of hydrocarbons in the suspended particulate matter.     

Evaluating crude oil chemical dispersion efficacy in a flow-through wave tank under regular non-breaking wave and breaking wave conditions

Testing dispersant effectiveness under conditions similar to that of the open environment is required for improvements in operational procedures and the formulation of regulatory guidelines. To this end, a novel wave tank facility was fabricated to study the dispersion of crude oil under regular non-breaking and irregular breaking wave conditions. This wave tank facility was designed for operation in a flow-through mode to simulate both wave- and current-driven hydrodynamic conditions. We report here an evaluation of the effectiveness of chemical dispersants (Corexit^® EC9500A and SPC 1000^™) on two crude oils (Medium South American [MESA] and Alaska North Slope [ANS]) under two different wave conditions (regular non-breaking and plunging breaking waves) in this wave tank. The dispersant effectiveness was assessed by measuring the water column oil concentration and dispersed oil droplet size distribution. In the absence of dispersants, nearly 8-19% of the test crude oils were dispersed and diluted under regular wave and breaking wave conditions. In the presence of dispersants, about 21-36% of the crude oils were dispersed and diluted under regular waves, and 42-62% under breaking waves. Consistently, physical dispersion under regular waves produced large oil droplets (volumetric mean diameter or VMD ? 300 μm), whereas chemical dispersion under breaking waves created small droplets (VMD ? 50 μm). The data can provide useful information for developing better operational guidelines for dispersant use and improved predictive models on dispersant effectiveness in the field.     

The Arctic is no longer put on ice: Evaluation of Polar cod (Boreogadussaida) as a monitoring species of oil pollution in cold waters

The withdrawing Arctic ice edge will facilitate future sea transport and exploration activities in the area, which calls for the establishment of relevant cold water monitoring species. The present study presents first results of field baseline levels for core oil pollution biomarkers in Polar cod (Boreogadussaida) sampled from pristine, Arctic waters. Furthermore, biomarker response levels were characterized in controlled laboratory exposure experiments running over 2 weeks. Fish exposed to a simulated petrogenic spill (1 ppm dispersed, crude oil) exhibited elevated hepatic EROD activity, bile PAH-metabolites, and hepatic DNA-adducts, whereas male individuals exposed to simulated produced water (30 ppb nonylphenol) exhibited a strong induction of plasma vitellogenin. In conclusion, the results demonstrated low and robust biomarker baseline levels that were clearly different from exposure responses. In combination with its high abundance and circumpolar distribution, the Polar cod seems well qualified for oil pollution monitoring in Arctic waters.     

Measuring the effect of structural connectivity on the water dynamics in heterogeneous porous media using speedy neutron tomography

The temporal and spatial distribution of water within a porous medium is affected by the medium’s structure, i.e., the spatial arrangement of its constituents. To analyze structural effects on the fluid dynamics, we measured the 3D water content distribution in a heterogeneous sand column during two drainage-wetting cycles using neutron transmission tomography. The sample with a volume of 105 cm³ contained 101 cubes of fine and 49 cubes of coarse sand with particles ranging from 0.01 to 0.05 and 0.03 to 0.09 cm, respectively. The pressure at the lower boundary was determined by the water reservoir positioned between 7 and 39 cm below the top of the column. The duration of one complete 3D scanning with a spatial resolution of 127 μm was 56 s. The signal to noise ratio of the measurements was low due to the short exposure time in the neutron beam, but it was possible to quantify the water content in the individual cubes and hence the effect of structure on macroscopic water distribution. Continuous structures of coarse sand drained faster than coarse sand without connection to the upper boundary. During the initial wetting phase, cubes of coarse sand material completely embedded in the fine material remained water unsaturated due to air entrapment. The effect of the coarse sand connectivity was analyzed in two-dimensional numerical simulations based on Richards equation. In contrast to the measurements, no effect of structure connectivity was found. The coarse sand cubes embedded within the fine matrix drain as quickly as the coarse sand cubes arranged in a continuous channel due to the model assumption of a continuous air phase.     

Isolation and characterization of a novel hydrocarbon-degrading bacterium Achromobacter sp. HZ01 from the crude oil-contaminated seawater at the Daya Bay,southern China

Microorganisms play an important role in the biodegradation of petroleum contaminants, which have attracted great concern due to their persistent toxicity and difficult biodegradation. In this paper, a novel hydrocarbon-degrading bacterium HZ01 was isolated from the crude oil-contaminated seawater at the Daya Bay, South China Sea, and identified as Achromobacter sp. Under the conditions of pH 7.0, NaCl 3% (w/v), temperature 28 °C and rotary speed 150 rpm, its degradability of the total n-alkanes reached up to 96.6% after 10 days of incubation for the evaporated diesel oil. Furthermore, Achromobacter sp. HZ01 could effectively utilize polycyclic aromatic hydrocarbons (PAHs) as its sole carbon source, and could remove anthracene, phenanthrene and pyrence about 29.8%, 50.6% and 38.4% respectively after 30 days of incubation. Therefore, Achromobacter sp. HZ01 may employed as an excellent degrader to develop one cost-effective and eco-friendly method for the bioremediation of marine environments polluted by crude oil.     

BIOB: A mathematical model for the biodegradation of low solubility hydrocarbons

Modeling oil biodegradation is an important step in predicting the long term fate of oil on beaches. Unfortunately, existing models do not account mechanistically for environmental factors, such as pore water nutrient concentration, affecting oil biodegradation, rather in an empirical way. We present herein a numerical model, BIOB, to simulate the biodegradation of insoluble attached hydrocarbon. The model was used to simulate an experimental oil spill on a sand beach. The biodegradation kinetic parameters were estimated by fitting the model to the experimental data of alkanes and aromatics. It was found that parameter values are comparable to their counterparts for the biodegradation of dissolved organic matter. The biodegradation of aromatics was highly affected by the decay of aromatic biomass, probably due to its low growth rate. Numerical simulations revealed that the biodegradation rate increases by 3–4 folds when the nutrient concentration is increased from 0.2 to 2.0 mg N/L.     

Effects of temperature and wave conditions on chemical dispersion efficacy of heavy fuel oil in an experimental flow-through wave tank

The effectiveness of chemical dispersants (Corexit 9500 and SPC 1000) on heavy fuel oil (IFO180 as test oil) has been evaluated under different wave conditions in a flow-through wave tank. The dispersant effectiveness was determined by measuring oil concentrations and droplet size distributions. An analysis of covariance (ANCOVA) model indicated that wave type and temperature significantly (p < 0.05) affected the dynamic dispersant effectiveness (DDE). At higher temperatures (16 °C), the test IFO180 was effectively dispersed under breaking waves with a DDE of 90% and 50% for Corexit 9500 and SPC 1000, respectively. The dispersion was ineffective under breaking waves at lower temperature (10 °C), and under regular wave conditions at all temperatures (10-17 °C), with DDE < 15%. Effective chemical dispersion was associated with formation of smaller droplets (with volumetric mean diameters or VMD ? 200 μm), whereas ineffective dispersion produced large oil droplets (with VMD ? 400 μm).     

Recovery of interior brackish marshes seven years after the chalk point oil spill

Seven years after the April 2000 spill of 140,000 gallons of a mixture of No. 6 and No. 2 fuel oils in the Patuxent River, Maryland, heavily oiled brackish marshes showed continuing effects. Stem density and stem height were significantly lower in oiled versus unoiled sites for Spartina alterniflora but not Spartina cynosuroides habitats. In contrast, belowground biomass was significantly lower in S. cynosuroides habitats but not S. alterniflora habitats. Total PAH concentrations were up to 453 mg/kg in surficial soils (0-10 cm) and 2921 mg/kg with depth (10-20 cm). The oil had lost 22-76% of its initial PAH content after seven years, although the oil in marsh soils has undergone little to no additional weathering since Fall 2000. Based on amphipod acute toxicity tests and sediment quality guidelines, 25% of the soils in the marsh are expected to be toxic (ESB-TU_FCV values > 3.0; P_Max > 0.65).     

Phytoplankton production and physiological adaptation on the southeastern shelf of the Agulhas ecosystem

An investigation of phytoplankton production and physiology was undertaken during two research cruises on the southeastern shelf of southern Africa. The data set included photosynthesis-irradiance and active fluorescence parameters, phytoplankton absorption coefficients and HPLC pigment concentrations. Primary production was estimated to vary over a similar range for both cruises within 0.27–3.69 g C m⁻² d⁻¹. Pigment indices indicated that diatoms were dominant on the first cruise and the communities were subject to conditions where the mixed layer was deeper than the euphotic zone and they optimized their photosynthesis to very low light intensities at the bottom and below the euphotic zone. Mixed diatom-flagellate populations were observed during the second cruise where the euphotic zone was deeper than the mixed layer and the populations adapted to irradiances higher in the euphotic zone. In response to a mean lower water column PAR, it was found that these mixed communities increased the proportion of chlorophyll a in the pigment pool and had a higher quantum yield of photochemistry and higher light-limited photosynthetic efficiency.     

Produced water extracts from North Sea oil production platforms result in cellular oxidative stress in a rainbow trout in vitro bioassay

Produced water (PW) discharged from offshore oil industry contains chemicals known to contribute to different mechanisms of toxicity. The present study aimed to investigate oxidative stress and cytotoxicity in rainbow trout primary hepatocytes exposed to the water soluble and particulate organic fraction of PW from 10 different North Sea oil production platforms. The PW fractions caused a concentration-dependent increase in reactive oxygen species (ROS) after 1 h exposure, as well as changes in levels of total glutathione (tGSH) and cytotoxicity after 96 h. Interestingly, the water soluble organic compounds of PW were major contributors to oxidative stress and cytotoxicity, and effects was not correlated to the content of total oil in PW. Bioassay effects were only observed at high PW concentrations (3-fold concentrated), indicating that bioaccumulation needs to occur to cause similar short term toxic effects in wild fish.     

Droplet breakup in subsurface oil releases – Part 1: Experimental study of droplet breakup and effectiveness of dispersant injection

Size distribution of oil droplets formed in deep water oil and gas blowouts have strong impact on the fate of the oil in the environment. However, very limited data on droplet distributions from subsurface releases exist. The objective of this study has been to establish a laboratory facility to study droplet size versus release conditions (rates and nozzle diameters), oil properties and injection of dispersants (injection techniques and dispersant types). This paper presents this facility (6 m high, 3 m wide, containing 40 m³ of sea water) and introductory data. Injection of dispersant lowers the interfacial tension between oil and water and cause a significant reduction in droplet size. Most of this data show a good fit to existing Weber scaling equations. Some interesting deviations due to dispersant treatment are further analyzed and used to develop modified algorithms for predicting droplet sizes in a second paper (Johansen et al., 2013).     

Hydrocarbon composition and distribution in a coastal region under influence of oil production in northeast Brazil

Waters and sediments from the Potiguar Basin (NE Brazilian coast) were investigated for the presence and nature of polycyclic aromatic hydrocarbons (PAH) and aliphatic hydrocarbons. The region receives treated produced waters through a submarine outfall system serving the industrial district. The total dispersed/dissolved concentrations in the water column ranged from 10-50 ng L⁻¹ for ∑16PAH and 5-10 μg L⁻¹ for total aliphatic hydrocarbons. In the sediments, hydrocarbon concentrations were low (0.5-10 ng g⁻¹for ∑16PAH and 0.01-5.0 μg g⁻¹ for total aliphatic hydrocarbons) and were consistent with the low organic carbon content of the local sandy sediments. These data indicate little and/or absence of anthropogenic influence on hydrocarbon distribution in water and sediment. Therefore, the measured values may be taken as background values for the region and can be used as future reference following new developments of the petroleum industry in the Potiguar Basin.     

A case study of airborne and satellite remote sensing of a spring bloom event in the Gulf of Finland

The concentrations of chlorophyll-a (chl-a), total suspended solids (TSS) and the absorption coefficient of colored dissolved organic matter (a_CDOM(400)) are estimated in Case II waters using medium resolution imaging spectrometer (MERIS) satellite (full resolution [FR] level 1b, 300 m resolution) and AISA airborne spectrometer data acquired during a spring bloom in the Gulf of Finland, Baltic Sea on April 27, 2004. The accuracy of the estimation is analyzed using empirical band-ratio algorithms together with in situ observations that include water samples analyzed in a laboratory (variation ranges: 22–130 μg/l, 2.9–20 mg/l, and 1.29–2.61 m⁻¹ for chl-a, TSS and a_CDOM(400), respectively). Additional in situ estimates (transects) on these characteristics are available through absorption and scattering coefficients measured with an ac-9 absorption and attenuation meter installed in a flow-through system. The retrieval accuracy (R²) of all three water quality characteristics with MERIS data is close to or above 0.9, while the RMSE is 7.8 μg/l (22%), 0.74 mg/l (16%) and 0.08 m⁻¹ (5%), for chl-a, TSS and a_CDOM(400), respectively. The validity of the chl-a algorithm is tested using nine additional data points. The BIAS-error for these points is 5.2 μg/l and the RMSE is 10.6 μg/l. The effects of changes in the atmospheric characteristics on band-ratio algorithms in cases where no concurrent in situ reference data are available are analyzed using the MODerate spectral resolution atmospheric TRANSmittance algorithm and computer model (MODTRAN). The additional error due to these changes is estimated to be below 20% for the applied ratio algorithms. The water quality data available in the level 2 MERIS-product distributed by the European Space Agency did not include valid results for the date investigated here.     

Early spring turbulent mixing in an ice-covered Arctic fjord during transition to melting

Observations are presented of currents, hydrography and turbulence in a jet-type tidally forced fjord in Svalbard. The fjord was ice covered at the time of the experiment in early spring 2004. Turbulence measurements were conducted by both moored instruments within the uppermost 5 m below the ice and a microstructure profiler covering 3–60 m at 75 m depth. Tidal choking at the mouth of the fjord induces a tidal jet advecting relatively warmer water past the measurement site and dominating the variability in hydrography. While there was no strong correlation with the observed hydrography or mixing and the phase of the semidiurnal tidal cycle, the mean structure in dissipation of turbulent kinetic energy, work done under the ice and the mixing in the water column correlated with the current when conditionally sampled for tidal jet events. Observed levels of dissipation of turbulent kinetic energy per unit mass, 1.1×10⁻⁷ W kg⁻¹, and eddy diffusivity, 7.3×10⁻⁴ m² s⁻¹, were comparable to direct measurements at other coastal sites and shelves with rough topography and strong forcing. During spring tides, an average upward heat flux of 5 W m⁻² in the under-ice boundary layer was observed. Instantaneous (1 h averaged) large heat flux events were correlated with periods of large inflow, hence elevated heat fluxes were associated with the tidal jet and its heat content. Vertical heat fluxes are derived from shear-probe measurements by employing a novel model for eddy diffusivity [Shih et al., 2005. Parameterization of turbulent fluxes and scales using homogeneous sheared stably stratified turbulence simulations. Journal of Fluid Mechanics 525, 193–214]. When compared to the direct heat flux measurements using the eddy correlation method at 5 m below the ice, the upper 4–6 m averaged heat flux estimates from the microstructure profiler agreed with the direct measurements to within 10%. During the experiment water column was stably, but weakly, stratified. Destabilizing buoyancy fluxes recorded close to the ice were absent at 5 m below the ice, and overall, turbulence production was dominated by shear. A scaling for dissipation employing production by both stress and buoyancy [Lombardo and Gregg, 1989. Similarity scaling of viscous and thermal dissipation in a convecting boundary layer. Journal of Geophysical Research 94, 6273–6284] was found to be appropriate for the under-ice boundary layer.     

Velocity and turbulence variations at the edge of saltmarshes

Saltmarsh vegetation significantly influences tidal currents and sediment deposition by decelerating the water velocity in the canopy. In order to complement previous field results, detailed profiles of velocity and turbulence were measured in a laboratory flume. Natural Spartina anglica plants were installed in a 3 m length test section in a straight, recirculating flume. Different vegetation densities, water depths and surface velocities were investigated. The logarithmic velocity profile, which existed in front of the vegetation, was altered gradually to a skimming-flow profile, typical for submerged saltmarsh vegetation. The flow reduction in the denser part of the canopy also induced an upward flow (the current was partially deflected by the canopy). The skimming flow was accompanied by a zone of high turbulence co-located with the strongest velocity gradient. This gradient moved upward and the turbulence increased with distance from the edge of the vegetation. Below the skimming flow, the velocity and the turbulence were low. The structure of the flow in the canopy was relatively stable 2 m into the vegetation. The roughness length (z₀) of the vegetation depends only on the vegetation characteristics, and is not sensitive to the current velocity or the water depth. Both the reduced turbulence in the dense canopy and the high turbulence at the top of the canopy should increase sediment deposition. On the other hand, the high turbulence zone just beyond the vegetation edge and the oblique upward flow may produce reduced sedimentation; a phenomenon that was observed near the vegetation edge in the field.