Population dynamics of marine ciliate Euplotes vannus (Protozoa, Ciliophora) in different artificial seawaters

To study population dynamics of marine ciliates in different artificial seawaters (ASW), the population growth dynamics of a common marine ciliate Euplotes vannus were investigated using beef extract media and rice media for five types of ASW and natural seawater (NSW). The results show that: (1) the population growth rate was in the order of NSW>Flack ASW>Nakamula ASW>Schmadz ASW>Oshima ASW>Subow ASW and was considerably higher in rice media than in beef extract media (apart from Subow ASW); (2) the maximum density of E. vannus in stationary phase in each treatment was ranked as Flack ASW>Nakamula ASW>Schmadz ASW>NSW>Oshima ASW>Subow ASW, and was again higher in rice media than in beef extract media (except for Subow ASW); (3) the exponential and stationary phases were longer in rice media than in beef extract media; (4) strains of E. vannus that had been domesticated for >1 year in ASW grew significantly slower, with lower maximum density and longer stationary phase than those isolated and maintained in NSW. It was demonstrated that: (1) E. vannus may grow well in Flack, Nakamula and Schmads ASW compared with NSW (mainly in terms of growth rate); and (2) Oshima ASW is the preferred choice for stock cultures of E. vannus, but the ASWs Flack, Nakamula and Schmadz are preferred for mass culture. These findings suggest that these three ASWs are effective for the cultivation of marine protozoa for experimental studies on ecology, toxicology and molecular biology.     

Assessing spatio-temporal characteristics of water storage changes in the mountainous areas of Central Asia based on GRACE

The mountainous areas of Central Asia provide substantial water resources, and studying change in water storage and the impacts of precipitation and snow cover in the mountain ranges of Central Asia is of the greatest importance for understanding regional water shortages and the main factors. Data from the GRACE(Gravity Recovery and Climate Experiment) satellites, precipitation products and snow-covered area data were used to analyze the spatio-temporal characteristics of water storage changes and the effects of precipitation and snow cover from April 2002 to December 2013. The results were computed for each mountain ranges, and the following conclusions were drawn. The water storage in the mountainous areas of Central Asia as a whole increases in summer and winter, whereas it decreases in autumn. The water storage is affected by precipitation to some extent and some areas exhibit hysteresis. The area of positive water storage changes moves from west to east over the course of the year. The water storage declined during the period 2002–2004. It then returned to a higher level in 2005–2006 and featured lower levels in 2007–2009 Subsequently, the water storage increased gradually from 2010 to 2013. The Eastern Tianshan Mountains and Western Tianshan Mountain subzones examined in this study display similar tendencies, and the trends observed in the Karakorum Mountains and the Kunlun Mountains are also similar. However, the Eastern Tianshan Mountains and Western Tianshan Mountains were influenced by precipitation to a greater degree than the latter two ranges. The water storage in Qilian Mountains showed a pronounced increasing trend, and this range is the most strongly affected by precipitation. Based on an analysis of all investigated subzones, precipitation has the greatest influence on total water storage relative to the snow covered area in some areas of Central Asia. The results obtained from this study will be of value for scientists studying the mechanisms that influence changes in water storage in Central Asia.     

Regional variation in carbon sequestration potential of forest ecosystems in China

Enhancing forest carbon(C) storage is recognized as one of the most economic and green approaches to offsetting anthropogenic CO_2 emissions. However, experimental evidence for C sequestration potential(C_(sp)) in China's forest ecosystems and its spatial patterns remain unclear, although a deep understanding is essential for policy-makers making decisions on reforestation. Here, we surveyed the literature from 2004 to 2014 to obtain C density data on forest ecosystems in China and used mature forests as a reference to explore C_(sp). The results showed that the C densities of vegetation and soil(0–100 cm) in China's forest ecosystems were about 69.23 Mg C/ha and 116.52 Mg C/ha, respectively. In mature forests, the C_(sp) of vegetation and soil are expected to increase to 129.26 Mg C/ha(87.1%) and 154.39 Mg C/ha(32.4%) in the coming decades, respectively. Moreover, the potential increase of C storage in vegetation(10.81 Pg C) is estimated at approximately twice that of soil(5.01 Pg C). Higher C_(sp) may occur in the subtropical humid regions and policy-makers should pay particular attention to the development of new reforestation strategies for these areas. In addition to soil nutrients and environment, climate was an important factor influencing the spatial patterns of C density in forest ecosystems in China. Interestingly, climate influenced the spatial patterns of vegetation and soil C density via different routes, having a positive effect on vegetation C density and a negative effect on soil C density. This estimation of the potential for increasing forest C storage provided new insights into the vital roles of China's forest ecosystems in future C sequestration. More importantly, our findings emphasize that climate constraints on forest C sequestration should be considered in reforestation strategies in China because the effects of climate were the opposite for spatial patterns of C density in vegetation and soil.Enhancing forest carbon(C) storage is recognized as one of the most economic and green approaches to offsetting anthropogenic CO2 emissions. However, experimental evidence for C sequestration potential(Csp) in China's forest ecosystems and its spatial patterns remain unclear, although a deep understanding is essential for policy-makers making decisions on reforestation. Here, we surveyed the literature from 2004 to 2014 to obtain C density data on forest ecosystems in China and used mature forests as a reference to explore Csp. The results showed that the C densities of vegetation and soil(0–100 cm) in China's forest ecosystems were about 69.23 Mg C/ha and 116.52 Mg C/ha, respectively. In mature forests, the Csp of vegetation and soil are expected to increase to 129.26 Mg C/ha(87.1%) and 154.39 Mg C/ha(32.4%) in the coming decades, respectively. Moreover, the potential increase of C storage in vegetation(10.81 Pg C) is estimated at approximately twice that of soil(5.01 Pg C). Higher Csp may occur in the subtropical humid regions and policy-makers should pay particular attention to the development of new reforestation strategies for these areas. In addition to soil nutrients and environment, climate was an important factor influencing the spatial patterns of C density in forest ecosystems in China. Interestingly, climate influenced the spatial patterns of vegetation and soil C density via different routes, having a positive effect on vegetation C density and a negative effect on soil C density. This estimation of the potential for increasing forest C storage provided new insights into the vital roles of China's forest ecosystems in future C sequestration. More importantly, our findings emphasize that climate constraints on forest C sequestration should be considered in reforestation strategies in China because the effects of climate were the opposite for spatial patterns of C density in vegetation and soil.     

Application of rRNA probes and fluorescence in situ hybridization for rapid detection of the toxic dinoflagellate Alexandrium minutum

The dinoflagellate Alexandrium minutum is often associated with harmful algal blooms (HABs). This species consists of many strains that differ in their ability to produce toxins but have similar morphology, making identification difficult. In this study, species-specific rRNA probes were designed for whole-cell fluorescence in situ hybridization (FISH) to distinguish A. minutum from two phylogenetic clades. We acquired the complete SSU to LSU rDNA sequences (GenBank accession numbers JF906989-JF906999) of 11 Alexandrium strains and used these to design rRNA targeted oligonucleotide probes. Three ribotype-specific probes, M-GC-1, M-PC-2, and M-PC-3, were designed. The former is specific for the GC clade ("Global clade") of A. minutum, the majority of which have been found non-toxic, and the latter two are specific for the PSP (paralytic shellfish poisoning)-producing PC clade ("Pacific clade"). The specificity of these three probes was confirmed by FISH. All cells in observed fields of view were fluorescently labeled when probes and target species were incubated under optimized FISH conditions. However, the accessibility of rRNA molecules in ribosomes varied among the probe binding positions. Thus, there was variation in the distribution of positive signals in labeled cells within nucleolus and cytosol (M-GC-1, M-PC-3), or just nucleolus (M-PC-2). Our results provide a methodological basis for studying the biogeography and population dynamics of A. minutum, and providing an early warning of toxic HABs.     

Regimes of Runoff Components on the Debris-covered Koxkar Glacier in Western China

By using a degree-day based distributed hydrological model, regimes of glacial runoff from the Koxkar glacier during 2007-2011 are simulated, and variations and characteristics of major hydrological components are discussed. The results show that the meltwater runoff contributes 67.4%, of the proglacial discharge, out of which snowmelt, clean ice melting, buried-ice ablation and ice-cliff backwasting account for 22.4%, 21.9%, 17.9% and 5.3% of the total melt runoff, respectively. Rainfall runoff is significant in mid-latitude glacierized mountain areas like Tianshan and Karakorum. In the Koxkar glacier catchment, about 11.5% of stream water is initiated from liquid precipitation. Spatial distributions for each glacial runoff component reveal the importance of climatic gradients, local topography and morphology on glacial runoff generation, and temporal variations of these components is closely related to the annual cycle of catchment meteorology and glacier storage. Four stages are recognized in the seasonal variations of glacier storage, reflecting changes in meltwater yields, meteorological conditions and drainage systems in the annual hydrological cycle.     

Spatial and Temporal Variability of Thermal Stress to China’s Coral Reefs in South China Sea

Coral bleaching, caused by elevated sea surface temperature(SST), is occurring more frequently and seriously worldwide. Due to the lack of field observations, we understand little about the large-scale variability of thermal stress in the South China Sea(SCS) and its effect on China’s coral reefs. This paper used 4-km high resolution gap-filled SST(Filled SST) data and thermal stress data related to coral bleaching derived from Coral Reef Temperature Anomaly Database(Co RTAD) to quantify the spatial and temporal characteristics of chronic thermal stress and acute thermal stress to China’s coral reefs in SCS from 1982 to 2009. We analyzed the trend of SST in summer and the thermal stress frequency, intensity and duration during this period. The results indicate that, as a chronic thermal stress, summer mean SST in SCS shows an average upward trend of 0.2℃/decade and the spatial pattern is heterogeneous. Waters of Xisha Islands and Dongsha Islands of the northern SCS are warming faster through time compared to Zhongsha Islands and Nansha Islands sea areas of the southern SCS. High frequency bleaching related thermal stress events for these reefs are seen in the area to the northwest of Luzon Island. Severe anomaly thermal stress events are more likely to occur during the subsequent year of the El Nino year for these coral reefs. Besides, the duration of thermal stress varies considerably by anomaly year and by region.     

Cultural characteristics of chromium resistant unicellular cyanobacteria isolated from local environment in Pakistan

Many unicellular cyanobacteria were isolated from different places： fields, ponds, polluted water, and soils from Muredkey and Kasur tannery areas, near Lahore, Pakistan. Different media like BG 11 medium, Bold Basal medium, Chu＇s # 10 medium and Gotham＇s medium, in standard forms and with slight variations of ingredients, and different pH, temperature and light regimes were checked for the optimum growth of the isolates. The isolation pro- cedure was repeated with different concentrations of chromium to select the resistant strains. These selected strains grew on chromium of the range 100-200 μg/ml in BG 11 medium. Cyanobacteria were maintained in solid and liquid media with/without shaking. Cyanobacterial strains were collected from natural habitats that were accompanied by a diversified group of organisms including bacteria, protozoan, and rotifers etc. In order to eliminate these agents termed as contaminants, we used several methods including phenol treatment, use of antibiotic and careful manual picking of unicellular cyanobacteria. Resistance of these strains against different heavy metals （ZnSO4, MnSO4, NiSO4, COCl2, Pb（NO3）3, CuSO4, HgCl2, AgNO3 and CdCl2） and antibiotics （erythromycin, streptomycin, kanamycin, chloramphenicol, neomycin） was evaluated. Optimum temperature was 30℃ with variable pH for the reduction of Cr^6＋ in to Cr^3＋ in majority of strains.     

Revealing storage-area relationship of open water in ungauged subalpine wetland — Napahai in Northwest Yunnan, China

Mountains and plateaus in Southwest China contain many subalpine and alpine wetlands, with significant hydroecological functions. But ungauged or poorly gauged conditions limit the study and understanding of hydrological regimes of these wetland types. This study selects an ungauged subalpine wetland-Napahai in Northwest Yunnan, China-as a case for developing a practical approach to revealing its storage-area relationship of open water. A Trimble R8 GNSS (Global Navigation Satellites Systems) RTK (Real-time Kinematic system) and sonar fathometer were used to survey fine-resolution elevation data and generate a digital elevation model of the Napahai Wetland. Forty-four Landsat images from 1987 to 2011 were collected, and the Normalized Difference Water Index was used to classify open water features in the area. The area of open water in Napahai was calculated for each phase. With these data and a developed conceptual model, the storage of open water for each phase was estimated using ArcGIS tools. Both storage and area of open water showed significant intra-annual and inter-annual variations. In the rainy season, the monthly change of average storage of open water in Napahai showed about 1-2 months lag behind mean monthly rainfall. The storage-area relationship of open water was well fit by a power function equation (R 2 ≈0.91, n=44). This study indicates that if detailedelevations are available for similarly ungauged subalpine wetlands in Southwest China, researchers can use this practical approach to estimate multi- temporal areas and storages and reveal the storage-area relationship of open water in the wetlands. The study provided valuable information of this case wetland for optimizing its hydro-ecological managements and a new method to wetland researchers and managers for the hydrological study of similarly ungauged wetland complex.     

Tradeoffs and synergies between ecosystem services : A comparison of the karst and non-karst area

Under the influence of various natural and human factors, the relationship between the ecosystem services provided for human beings by a karst ecosystem is becoming increasingly complex, profoundly limiting the effective and sustainable development of the social economy and ecosystem protection in karst areas. Taking Guizhou, China as an example, which includes both karst(including five different landforms) and non-karst area, the study explored and compared the tradeoff and synergy between ecosystem services in both terrain types. The results showed higher change rates of water yield and soil retention in karst areas than those in non-karst areas, with only small differences in the carbon storage and crop production change. The ecosystem service relationships in the karst area from 1995 to 2005 were consistent with the relationships in the non-karst area. However, differences were observed in most of these relationships from 2005 to 2015. The relationships between ecosystem services in different karst landforms from 1995 to 2005 remained the same, but there are differences found in the relationships of ecosystem services from 2005 to 2015. The trade-off and synergistic relationships between ecosystem services in the different landforms were closely related to the changes of climate and land use, particularly related to rainfall, rainfall erosivity, farmland, and forestland.     

Molecular identification of Prorocentrum （Dinophyceae） species

A fragment of a large sub-unit ribosomal DNA (LrDNA) of 12 strains of Prorocentrum species was amplified by polymerase chain reaction (PCR). The PCR products were digested by 3 restriction endonucleases (Cfo Ⅰ, Hae Ⅲ, and RSA Ⅰ) and then resolved in agarose gels. Results show that different species had different RFLP patterns, except for P arcuatum (ME 131), which had the same pattern to P. micans (ME 160 and 04).The same fragment of 19 strains of the genus was also amplified and subjected to denaturing gradient gel electrophoresis (DGGE). 11 different patterns were resolved. Different cultures of a same species had the same pattern. The results of RFLP and DGGE analyses showed that eight newly isolated epibenthic Prorocentrum species were different from each other, and also from other cultured ones examined in this study. P arcuatum(MEI32) could not be differentiated from P. micans (MEI60 and 04), it was probably mis-identified, since they are quite different morphologically. P. redfieldii (MEI38) could also not be distinguished form P. triestinium(MEI32), it should be regarded as a synonym ofP. triestinium. Unexpectedly, a restriction site was found in P.micans, compared with previous sequence data.     

GENETIC VARIATION IN PARTHENOGENETIC ARTEMIA FROM THE SHANDONG PENINSULA, P.R.C.

Results are presented of a survey of isozyme frequencies in parthenogenetic Artimia from six salterns in the Shandong Peninsula, P.R.C. Three of the eleven enzymes we scored using polyacryamide gel electrophoresis proved to be useful for analysis of electromorph frequency variation. These enzymes are tetrazolium oxidase (TO), lactate dehydrogenase (LDH), and malate dehydrogenase (MDH). Each of these systems showed extensive isozyme variation both within and between salterns. In addition, we have examined the possibility that electromorph frequencies may vary as the result of natural selection for adaptation to specific salinity, or salinity-associated environmental conditions. An indication of clinal variation was found in two series of ponds differing in salinity, however more extensive data are needed before it is possible to conclude that these patterns are the result of natural selection. Finally, the use of isozyme analyses such as ours, for unraveling taxonomic problems in Anemia, is discussed.     

LIPIDS IN SEDIMENTS FROM BEIBU GULF , SOUTH CHINA SEA AND THEIR GEOCHEMICAL SIGNIFICANCE

Examination of solvent-extractable lipids in sediments from Beibu Gulf, South China Sea by capillary gas chromatography (GC) and capillary gas chromatography-mass spectrometry (GC-MS) resulted in the first identifications of β,γ-carotanes and their C19 to C31 homologs in the modern sediments from the China Seas. Distributional features of these ( mainly triterpenoid and steroid hydrocarbons) biomarkers are discussed and the possibility of using these compounds as indicators of source of materials, diagenesis and depositional conditions is explored. It was found that the biomarkers in this region are mixtures of allochthonous (terrigenous and / or crude oil leak or pollu -tion) and autochthonous (marine) organic components. The biomarkers have proved to be of value for understanding sedimentation .     

Dynamic Assessment on Regional Eco-environmental Quality Using AHP-Statistics Model—-A Case Study of Chaohu Lake Basin

An indicator system closely connected with eco-environment, in which indicator involves in such fields as society, economy, resources, environment and ecology, is first proposed for eco-environmental quality assessment. Then, a hierarchical model with four levels is established by virtue of these indicators and attributes. In the model, weights of indicators and attributes are determined by combining Delphi method with the Analytic Hierarchy Process (AHP) and a statistic method is used to eliminate the influences arising from the differences in dimension and magnitude of indicators. On these grounds, an AHP-statistics model is provided for regional eco-environmental quality assessment. As a case, such AHP-statistics model is utilized in the dynamic analysis of regional eco-environmental assessment in Chaohu Lake basin. Study results show that natural environmental quality in the watershed was in the declining state while social environmental quality was in a markedly improved situation from 1996, and the synthetic eco-environmental quality was gradually and slowly improved under the common influences of both natural and social environmental factors. Example of application testified the capacities of above methodology to evaluate the real and dynamic state of regional eco-environmental quality.     

Differentiation of the Agonists and Antagonists of the α7 Nicotinic Acetylcholine Receptor

The α7 nicotinic acetylcholine receptors(nAChRs) are widely expressed in the central and peripheral nervous systems and are important drug targets for the treatment of neurological diseases. However, differentiation of the agonists and antagonists of the nAChR is difficult. In this study we aimed to develop a reliable and efficient computational approach for differentiation of the agonists from the antagonists of the nAChR based on a systematical analysis of 123 ligands(87 agonists, 12 partial agonists, and 24 antagonists) binding with the extracellular domain of the α7 n AChR chimera. Our results suggest that the ligand size and ligand binding affinity cannot differentiate the agonists from the antagonists of the nAChR. The ligand efficiency that considers both ligand binding affinity and size for the agonists is overall more left shifted in comparison to the antagonists, but the values of the ligand efficiency still cannot differentiate the agonists from the antagonists unless the values are either relatively high(more than-0.3 kcal mol-1) or relatively low(less than-0.45 kcal mol-1). Our results suggest that accurate prediction of the agonist or antagonist of the nAChR is challenging and the ligand innate configuration has to be considered as an extra for differentiation of the agonists from the antagonists of the nAChR.     

Provenance and Tectonic Implications of Paleozoic Strata in the South Yellow Sea Basin,China–Revealed from the Borehole CSDP-2

Well CSDP-2 is a fully coring deep drilling hole, penetrating the thick Paleozoic marine strata in the South Yellow Sea Basin(SYSB) in the lower Yangtze Block(LYB). Based on the petrological and geochemical analysis of 40 sandstone samples from the core CSDP-2, the provenance and tectonic features of Paleozoic detrital rocks from SYSB are analyzed and systematically delineated in this article. The results show that the Silurian – Carboniferous sandstones are mature sublitharenite, while the Permian sandstones are unstable feldspathic litharenite. The average CIA(chemical index of alteration) is 74.61, which reflects these sediments were derived from source rocks with moderate chemical weathering. The REE(rare earth element) patterns are characterized by LREE enrichment, flat HREE and obviously negative Eu anomaly, which are similar to that of the upper continental crust. Dickinson QFL discrimination results indicate the recycled orogeny provenance. Various diagrams for the discrimination of sedimentary provenance based on major and trace element data show all the sediments were derived predominantly from quartz sedimentary rocks, of which the Permian strata contain more felsic sedimentary rocks. Geochemical data for these detrital rocks suggest they occur at the passive continental margin and island arc settings, and the Permian sandstone presents active continental margin setting.     

Stability and changes in astaxanthin ester composition from Haematococcus pluvialis during storage

In this paper, we investigated the effects of temperature, oxygen, antioxidants, and corn germ oil on the stability of astaxanthin from Haematococcus pluvialis under different storage conditions, and changes in the composition of astaxanthin esters during storage using high performance liquid chromatography and spectrophotometry. Oxygen and high temperatures （22-25~C） significantly reduced the stability of astaxanthin esters. Corn germ oil and antioxidants （ascorbic acid and vitamin E） failed to protect astaxanthin from oxidation, and actually significantly increased the instability of astaxanthin. A change in the relative composition of astaxanthin esters was observed after 96 weeks of long-term storage. During storage, the relative amounts of free astaxanthin and astaxanthin monoesters declined, while the relative amount of astaxanthin diesters increased. Thus, the ratio of astaxanthin diester to monoester increased, and this ratio could be used to indicate if astaxanthin esters have been properly preserved. If the ratio is greater than 0.2, it suggests that the decrease in astaxanthin content could be higher than 20%. Our results show that storing algal powder from H. pluvialis or other natural astaxanthin products under vacuum and in the dark below 4~C is the most economical and applicable storage method for the large-scale production of astaxanthin from H. pluvialis. This storage method can produce an astaxanthin preservation rate of at least 80% after 96 weeks of storage.     

A new approach to estimating the T-year return-period wave height

The paper introduces a new approach to estimating the T-year return-period wave height (TRPW), i.e. the wave height expected to occur in T-year, from two sets of observed extreme data and on the basis of the maximum entropy principle. The main points of the approach are as follows. 1) A maximum entropy probability density function (PDF) for the extreme wave height H is derived from a Euler equation subject to some necessary and rational constraints. 2) The parameters in the function are expressed in terms of the mth moment of H. 3) This PDF is convenient to theoretical and practical applications as it is simple and its four parameters are easy to be determined from observed extreme data. An example is given for estimating the TRPW in 50 and 100 years by the present approach and by some currently used methods using observed data at two hydrographic stations.The comparison of the estimated results shows that the present approach is quite similar to the Pearson-Ⅲ and Gumbel methods.     

Effects of Forest Type and Urbanization on Carbon Storage of Urban Forests in Changchun,Northeast China

Rapid urbanization has led to dramatic changes in urban forest structures and functions, and consequently affects carbon(C) storage in cities. In this study, field surveys were combined with high resolution images to investigate the variability of C storage of urban forests in Changchun, Northeast China. The main objectives of this study were to quantify the C storage of urban forests in Changchun City, Northeast China and understand the effects of forest type and urbanization on C storage of urban forests. The results showed that the mean C density and the total C storage of urban forests in Changchun were 4.41 kg/m2 and 4.74 × 108 kg, respectively. There were significant differences in C density among urban forest types. Landscape and relaxation forest(LF) had the highest C density with 5.41 kg/m2, while production and management forest(PF) had the lowest C density with 1.46 kg/m2. These differences demonstrate that urban forest type is an important factor needed to be considered when the C storage is accurately estimated. Further findings revealed significant differences in different gradients of urbanization, and the mean C density decreased from the first ring(6.99 kg/m2) to the fourth ring(2.87 kg/m2). The total C storage increased from the first ring to the third ring. These results indicate that C storage by urban forests will be significantly changed during the process of urbanization. The results can provide insights for decision-makers and urban planners to better understand the effects of forest type and urbanization on C storage of urban forests in Changchun, and make better management plans for urban forests.     

Diversity and bioactivity of actinomycetes from Signy Is- land terrestrial soils,maritime Antarctic

The Antarctic represents a largely untapped source for isolation of new microorganisms with potential to produce bio- active natural products. Actinomycetes are of special interest among such microorganisms as they are known to produce a large number of natural products, many of which have clinical, pharmaceutical or agricultural applications. We isolated, characterized and classified actinomycetes from soil samples collected from different locations on Signy Island, South Orkney Islands, in the maritime Antarctic. A total of 95 putative actinomyeete strains were isolated from eight soil samples using eight types of selective isolation media. The strains were dereplicated into 16 groups based on morphology and Amplified Ribosomal DNA Restriction Analysis （ARDRA） patterns. Analysis of 16S rRNA gene sequences of representatives from each group showed that streptomy- cetes were the dominant actinomycetes isolated from these soils; however, there were also several strains belonging to diverse and rare genera in the class Actinobacteria, including Demetria, Glaciibacter, Kocuria, Marmoricola, Nakamurella and Tsukamurella. In addition, screening for antibacterial activity and non-ribosomal peptide synthetase genes showed that many of the actinomycete strains have the potential to produce antibacterial compounds.     

Coupling of an individual-based model of anchovy with lower trophic level and hydrodynamic models

Anchovy (Engraulis japonicus), a small pelagic fish and food of other economic fishes, is a key species in the Yellow Sea ecosystem. Understanding the mechanisms of its recruitment and biomass variation is important for the prediction and management of fishery resources. Coupled with a hydrodynamic model (POM) and a lower trophic level ecosystem model (NEMURO), an individual-based model of anchovy is developed to study the influence of physical environment on anchovy’s biomass variation. Seasonal variations of circulation, water temperature and mix-layer depth from POM are used as external forcing for NEMURO and the anchovy model. Biomasses of large zooplankton and predatory zooplankton which anchovy feeds on are output from NEMURO and are controlled by the consumption of anchovy on them. Survival fitness theory related to temperature and food is used to determine the swimming action of anchovy in the model. The simulation results agree well with observations and elucidate the influence of temperature in over-wintering migration and food in feeding migration.