Distribution and origin of biologically available phosphorus in the water of the Meiliang Bay in summer

The investigation and continuous monitoring with an innovative iron oxide embedded cellulose acetate membrane (FeO/CAM) on the concentrations of biologically available phosphorus (BAP) were conducted in the Meiliang Bay of the Taihu Lake during summer in 2004. The results showed that the concentrations of dissolved (FeO-DP), particulate (FeO-PP) and total bioavailable phosphorus (FeO-P) had similar horizontal distribution. The BAP concentrations were the highest in those estuaries in the northern bay. With the decrease of the distance to the estuary or long shore, there was little difference between BAP concentrations in an open lake area. During the observation period, algal blooms occurred in most waters of the northern bay, which was reflected from the high concentrations of chlorophyll a (Chl-a). While they were not highest in the estuarine waters of those major rivers, this is the case for the BAP concentrations. The concentrations of Chl-a had a significantly positive correlation with those of bioavailable phosphorus in the open area of the Meiliang Bay. With the sediment resuspension induced by wind and wave, BAP concentrations increased in a short-term, indicating that the riverine P inputs mainly contribute to the concentrations of BAP in the estuarine water while internal P release was the major source of BAP in the open lake area. In the eutrophic shallow lake, the blooms of alga may cause pH increase and further result in internal P release. The above results showed that the new membrane of FeO/CAM can be used to monitor the concentrations of BAP and provide the scientific justifications for the control strategy of the lake eu-trophication.     

Evidence from isotopic geochemistry as an indicator of eutrophication of Meiliang Bay in Lake Taihu,China

In this paper, Lake Taihu, a large shallow freshwater lake in China, is chosen as an example of reconstruction of eutrophication through the comparison between stable isotopes from dissolved nutrients and plants and water column nutrient parameters and integration of multiple proxies in a sediment core from Meiliang Bay including TN, TP, TOC, C/N, δ ¹⁵N, δ ¹³C, etc. Differences in aquatic plant species and trophic status between East Taihu Bay and Meiliang Bay are indicated by their variations in δ ¹³C and δ ¹⁵N of aquatic plants and δ ¹⁵N of NH₄ ⁺. A significant influence of external nutrient inputs on Meiliang Bay is reflected in temporal changes in δ ¹⁵N of NH₄ ⁺ and hydro-environmental parameters. The synchronous change between δ ¹³C and δ ¹⁵N values of sedimented organic matter (OM) has been attributed to elevated primary production at the beginning of eutrophication between 1950 and 1990, then recent inverse correlation between them has been caused by the uptake of ¹⁵N-enriched inorganic nitrogen by phytoplankton grown under eutrophication and subsequent OM decomposition and denitrification in surface sediments, indicating that the lake has suffered from progressive eutrophication since 1990. Based on the use of a combination of stable isotopes and elemental geochemistry, the eutrophication of Meiliang Bay in Lake Taihu could be better traced. These transitions of the lake eutrophication respectively occurring in the 1950s and 1990s have been suggested as a reflection of growing impacts of human activities, which is coincident with the instrumental data.     

太湖梅梁湾水土界面反硝化和厌氧氨氧化

运用无扰动芯样实验室内流动培养、稳定同位素示踪、同位紊气态产物测定及同位素配对技术,对太湖梅梁湾北部到南部的4个梯度样点的水土界面反硝化和厌氧氨氧化速率进行研究.结果表明,梅梁湾内及湾外开敞湖区4个样点的水土界面反硝化脱氮速率为(46.36±13.26)-(16.34±22,74)μmol/(m~2·h),厌氧氨氧化脱氮速率为(7.50±2.21)-(2.05±2.90)～mol/(m~2.b).梅梁湾北部河口区水土界面总脱氮能力明显高于梅梁湾南部及开敞湖区.通过对脱氮过程的进一步研究发现.北部脱氮过程主要以上覆水硝酸盐为底物的非耦合反硝化过程(D_w)为优势过程,而梅梁湾外开敞湖区则以沉积物硝化过程耦合控制的反硝化(D_n)为主.影响D_n、D_w在反硝化中比重的主要因素是沉积物溶氧侵蚀深度和上覆水NO_3~-.浓度的差异;梅梁湾厌氧氨氧化脱氮比例占总脱氮比例为12%-14%,湾外开敞湖区则占11%,影响其比例差异的主要因子是反硝化强度的大小及其反硝化中间产物--亚硝酸盐含量的差异.     

Horizontal distribution and transport processes of bloom-forming Microcystis in a large shallow lake (Taihu, China)

The horizontal distribution of bloom-forming Microcystis in a specific lake area and the transport of Microcystis by wind-driven lake currents between Meiliang Bay and the open water of Taihu Lake were measured during continuous field observations from August 21 to 25, 2006. During the observations, the horizontal distributions of Microcystis, represented by Chlorophyll a, showed a clear concentration toward downwind lake areas. According to the lake currents and the Chl a concentrations on the boundary line between the Meiliang Bay and the open water, the transported Chl a was less than 2% of the total weight of Chl a in Meiliang Bay on August 22, 24 and 25. The results suggest that the horizontal distribution of the bloom-forming Microcystis was strongly affected by the wind conditions, and the wind-driven Microcystis accumulation was mainly determined by surface drift; the transport of Microcystis by lake currents was much less important in this large, shallow lake.     

Spatiotemporal variations of internal P-loading and the related mechanisms in the large shallow Lake Chaohu

Spatiotemporal variations of P species and adsorption behavior in water column, interstitial water, and sediments were investigated in the large shallow eutrophic Lake Chaohu. Orthophosphate (Ortho-P) and total phosphorus (TP) concentrations were significantly higher in the western part than in the eastern part of the lake, due to different nutrient inputs from the surrounding rivers. Moreover, particulate phosphorus (PP) concentration was in a similar spatial pattern to Ortho-P and TP concentrations, and also showed significantly positive correlation with the biomass of Microcystis, indicating more uptake and store of phosphorus by Microcystis than by other algae. Increase of pH and intensive utilization of P by phytoplankton were the main factors promoting P (especially Fe-P) release from the sediment to interstitial water during the cyanobacterial blooms in Lake Chaohu. Spatial dynamics in TP concentration, P species and adsorption behavior of the sediment, coupled with the statistical analyses, suggested that the spatial heterogeneity of P contents in the sediment was influenced by various factors, e.g. human activities, soil geochemistry and mineral composition. In spite of similar TP contents in the sediments, increase in proportion of Fe-P concentration in the sediment may result in a high risk of P release.     

Water level as the main driver of the alternation between a free-floating plant and a phytoplankton dominated state: a long-term study in a floodplain lake

This 10-year field data study explores the relevance of water level fluctuations in driving the shift from a free-floating plant (FFP) to a phytoplankton dominated state in a shallow floodplain lake from the Lower Paraná River. The multi-year natural flood pulse pattern in the Lower Paraná River drove the ecosystem regime from a FFP-dominant state during very high waters (1998–1999) to absolute phytoplankton prevalence with blooms of nitrogen fixing Cyanobacteria during extreme low waters (2008–2009). Satellite images support the observed changes over the decade and show the decrease of the surface lake area covered by FFP as well as the modification of the spectral firm in open waters, which documents the significant increases in phytoplankton chlorophyll a concentrations. We discuss the possibility that, despite a slow eutrophication in these highly vegetated systems, water level changes and not nutrients account for the shift from a floating macrophyte community to phytoplankton dominance. Cyclic shifts may occur in response to the seasonal floodpulse, but more strongly, as indicated by our results, in association to the extreme drought and flood events related to the El Niño Southern Oscillation, which is linked to discharge anomalies in the Paraná River.     

望虞河引长江水入太湖水体的总磷、总氮分析

太湖流域实施的调水引流,提高了流域水资源和水环境承载能力,发挥了水利工程在改善水环境方面的综合效益,支撑了流域经济社会的可持续发展.本文在分析近年来望虞河引江水量与入湖水量及入湖水体流经太湖湖湾水体水质变化情势的基础上,分析比较了2007年以来的调水引流期间,望虞河入太湖水体总磷、总氮浓度值与太湖贡湖湾、梅梁湾、湖西及江苏省其它主要入太湖河道的总磷、总氮浓度值,并通过监测结果分析了入太湖水体总磷、可溶性总磷的衰减趋势,从而得出,长江是优质水源,调引长江水为增加太湖水环境容量、改善太湖及区域水环境状况起到了积极作用.     

太湖蓝藻碎屑对水质及附着藻和水丝蚓生物量的影响

富营养化是现今各国面临的主要水环境问题,其中蓝藻水华暴发是全球富营养化水体最常见的现象之一.蓝藻水华将产生大量的蓝藻碎屑,其对水质及生物的影响还尚不清楚.本研究通过向中宇宙系统添加微囊藻碎屑,分析其对水体不同形态营养盐及水生生物生物量的影响.结果表明:微囊藻碎屑加入后,水体不同形态的营养盐浓度均在短期内迅速增加,其中水体总氮和总磷平均浓度最高分别达到3.86和0.36 mg/L;浮游植物生物量(用叶绿素a表示)在前9天随营养盐浓度的升高而增加,随后逐渐下降至实验初始水平.此外,附着藻类生物量在微囊藻碎屑加入后呈逐渐下降趋势,这可能与浮游植物快速增殖引起的水体透明度下降有关.微囊藻碎屑加入后,水丝蚓生物量随微囊藻碎屑的分解持续增长,在第20天达到生物量最大值.本研究通过模拟太湖梅梁湾生态系统,探讨微囊藻碎屑对水质及水生生物生物量的影响,结果有助于进一步了解蓝藻水华对水生态系统影响的途径及机理,为富营养化湖泊管理提供理论依据.     

Contributions of phosphatase and microbial activity to internal phosphorus loading and their relation to lake eutrophication

Phosphatase may accelerate the process of lake eutrophication through improving phosphorus bioavailability. This mechanism was studied in three Chinese eutrophic shallow lakes (Lake Taihu, Lake Longyang and Lake Lianhua). Phosphatase activity was related to the concentration of soluble reactive phosphorus (SRP) and chlorophyll a. Stability of dissolved phosphatase in reverse micelles may be attributed to molecular size, conformation and active residues of the enzyme. At the site with Microcystis bloomed in Lake Taihu, dissolved phosphatase activity was higher and more stable in micelles, SRP concentrations were lower in interstitial water, the contents of different forms of phosphorus and the amounts of aerobic bacteria were lower while respiration efficiency was higher in sediments. Phosphobacteria, both inorganic and organic and other microorganisms were abundant in surface water but rare in sediments. Therefore, internal phosphorus may substantially flux into water column by enzymatic hydrolysis and anaerobic release, together with mobility of bacteria, thereby initiating the bloom. In short, biological mechanism may act in concert with physical and chemical factors to drive the internal phosphorus release and accelerate lake eutrophication.     

太湖表层沉积物磷的吸附容量及其释放风险评估

利用沉积物磷吸附指数(PSI)和磷吸附饱和度(DIS)等指标来表征太湖表层沉积物的磷吸附容量,并探讨了太湖沉积物磷吸附容量的空间变化．研究发现：草酸铵提取的磷以及活性铁、铝氧化物含量在梅梁湾东北部至五里湖一带的沉积物中较高,而在太湖南部湖区相对较低,这主要是与附近城市污水的实际贡献有着密切关系;太湖沉积物的磷吸附指数大体上有着北高南低、西高东低的分布特征,而磷吸附饱和度分布与PSI恰有相反的特征;沉积物中磷的吸附容量可能主要受到无定形的铁和铝的氧化物控制,也受沉积物有机质含量的影响．初步确定了用磷吸附指数和磷吸附饱和度来表征的湖泊沉积物磷释放风险指数概念,并应用于对太湖沉积物磷诱发的富营养化风险的评估．     

Spatial and temporal variations in nutrient and chlorophyll-a concentrations in the northern East China Sea surrounding Cheju Island

Nutrients, chlorophyll-a (Chl-a), and environmental conditions were extensively investigated in the northern East China Sea (ECS) near Cheju Island during five research cruises from 2003 to 2007. In the eastern part of the study area, surface waters were characterized only by the Tsushima Current Water (TCW) during all five cruises. However, the western surface waters changed with season and were characterized by the Yellow Sea Cold Water (YSCW) in spring, the Changjiang Diluted Water (CDW) in summer, and the Yellow Sea Mixed Water (YSMW) in autumn. In spring and autumn, relatively high concentrations of nitrate and phosphate were observed in the surface waters in the western part of the study area, where vertical mixing brought large supplies of nutrients from deep waters. Changes in wind direction occasionally varied the inflow of the Changjiang plume in summer, clearly causing the annual variation in surface nitrate and phosphate concentrations in summer. In summer, the surface distribution of nitrate and phosphate did not coincide with that of silicate in the study area, which probably resulted from the significant drop in the Si:N ratio in the Changjiang plume since construction of the Three Gorges Dam (TGD). Despite large temporal and spatial variations in surface Chl-a concentrations, depth-integrated Chl-a concentrations exhibited little variation temporally and spatially. In the study area, surface Chl-a concentration did not well reflect the standing stocks of phytoplankton. The vertical distribution of Chl-a showed large temporal and spatial variations, and the main factor controlling the vertical distribution of Chl-a in summer was the availability of nitrate. The thermohaline front may play an important role for accumulation of phytoplankton biomass in spring and autumn.     

Sedimentary and geochemical evidence of Eocene climate change in the Xining Basin,northeastern Tibetan Plateau

The northeastern Tibetan Plateau began to grow during the Eocene and it is important to understand the climatic history of Asia during this period of so-called ‘doubthouse' conditions. However, despite major advances in the last few decades,the evolutionary history and possible mechanisms of Eocene climate change in the northeastern Tibetan Plateau remain unclear.The Xining Basin in the northeastern Tibetan Plateau contains a continuous sequence of Early to Late Eocene non-marine sediments which provides the opportunity to resolve long-term climate changes during this period. In this study, we report the results of analyses of lithofacies, sediment color and geochemistry of bulk samples collected from the Xijigou section of the Xining Basin. An abrupt lithofacies change between the Early(~52–40 Ma) and Late Eocene(~40–34 Ma) indicates a change in the depositional environment from a shallow lake to a playa lake in response to a significant climatic shift. During ~52–40 Ma,higher values of sediment redness(a*), redness/lightness(a*/L*) and higher modified Chemical Index of Weathering(CIW′)indicate a relatively warm and humid climate, while from ~40–34 Ma the lower values of a*, a*/L*and lower CIW′ imply subhumid to semi-arid climatic conditions. The paleoclimatic records indicate a long-term(~52–34 Ma) trend of decreasing chemical weathering, consistent with global climate change. An abrupt sharp excursion of the proxy records during ~42–40 Ma suggests a relatively brief warm interval, corresponding to the Middle Eocene Climatic Optimum(MECO). We suggest that global cooling substantially reduced humidity in inner Asia, resulting in sub-humid to semi-arid climatic conditions after 40 Ma in the Xining Basin, which may have been responsible for the long-term trend of decreasing chemical weathering during the Eocene.     

Energy input and dissipation in a temperate lake during the spring transition

ADCP and temperature chain measurements have been used to estimate the rate of energy input by wind stress to the water surface in the south basin of Windermere. The energy input from the atmosphere was found to increase markedly as the lake stratified in spring. The efficiency of energy transfer (Eff), defined as the ratio of the rate of working in near-surface waters (RW) to that above the lake surface (P ₁₀), increased from ～0.0013 in vertically homogenous conditions to ～0.0064 in the first 40 days of the stratified regime. A maximum value of Eff～0.01 was observed when, with increasing stratification, the first mode internal seiche period decreased to match the diurnal wind period of 24 h. The increase in energy input, following the onset of stratification was reflected in enhancement of the mean depth-varying kinetic energy without a corresponding increase in wind forcing. Parallel estimates of energy dissipation in the bottom boundary layer, based on determination of the structure function show that it accounts for ～15% of RW in stratified conditions. The evolution of stratification in the lake conforms to a heating stirring model which indicates that mixing accounts for ～21% of RW. Taken together, these estimates of key energetic parameters point the way to the development of full energy budgets for lakes and shallow seas.     

太湖不同生态类型湖区浮游甲壳动物群落结构季节变化比较

水体富营养化会对整个水生态系统产生重要影响.为了解太湖富营养化对浮游甲壳动物群落结构的影响,于2012年3月至2013年2月对太湖3个典型湖区——藻型湖区(梅梁湾)、草型湖区(胥口湾)和强扰动湖区(湖心区)开展浮游甲壳动物群落结构季节变化比较研究.3个湖区中,湖心区营养水平最高,胥口湾最低.梅梁湾浮游甲壳动物密度和生物量最高,其次是湖心区,胥口湾最低.梅梁湾、湖心区和胥口湾的浮游甲壳动物年平均密度分别为199、150和91 ind./L,年平均生物量分别为1.950、1.557和0.743 mg/L.在整个研究期间,梅梁湾、胥口湾和湖心区的浮游甲壳动物种类数分别为13、11和11;3个湖区的浮游甲壳动物优势种均为中华窄腹剑水蚤和简弧象鼻溞,其中中华窄腹剑水蚤在梅梁湾、胥口湾和湖心区的年平均密度分别为57、25和36 ind./L,简弧象鼻溞在3个湖区的年平均密度分别为40、22和32 ind./L.胥口湾浮游甲壳动物生物多样性指数显著低于梅梁湾和湖心区.相关分析表明,浮游甲壳动物密度与叶绿素a浓度呈极显著正相关.研究表明,同一湖泊不同生态类型湖区浮游甲壳动物会对水体富营养化产生不同的生态响应.     

太湖1989—1993年富营养化资料的分析

The water quality of Lake Taihu has declined markedly in the past two or three decades. We used modern non-parametric statistical methods to analyse the water quality record for 1989-1993 (samples collected at 2-monthly intervals). Phytoplankton blooms have been a particular problem, as the very high peak chlorophyll a concentrations in the Meiliang (up to 0.4 g·m^-3) and the coastal regions (up to 0.5 g·m^-3) indicate. However, over a large area of the middle of the lake, peak chlorophyll a concentrations were much lower (0.01-0.03 g·m^-3). In this deeper, turbid part of the lake there may often be insufficient underwater light to support rapid phytoplankton growth; grazing by the abundant benthic bivalves may also be important. Total phosphorus (P) concentrations in the coastal waters increased significantly (p < 5%). Suspended solids concentrations also increased significantly, and as a result transparency decreased. However, phytoplankton probably usually only account for a small proportion of the suspended material, so the parallel increase in total P and suspended solids may be coincidental. Significant increases in chlorophyll a were not observed, but phytoplankton blooms are often short-lived (e.g. days to weeks), and thus may not always be detected by the 2-monthly sampling.     

Regeneration and utilization of nutrients during collapse of a <Emphasis Type="Italic">Mesodinium rubrum</Emphasis> red tide and its influence on phytoplankton species composition

High-biomass red tides occur frequently in some semi-enclosed bays of Hong Kong where ambient nutrients are not high enough to support such a high phytoplankton biomass. These high-biomass red tides release massive inorganic nutrients into local waters during their collapse. We hypothesized that the massive inorganic nutrients released from the collapse of red tides would fuel growth of other phytoplankton species. This could influence phytoplankton species composition. We tested the hypothesis using a red tide event caused by Mesodinium rubrum (M. rubrum) in a semi-enclosed bay, Port Shelter. The red tide patch had a cell density as high as 5.0×10⁵ cells L^?1, and high chlorophyll a (63.71 μg L^?1). Ambient inorganic nutrients (nitrate: \(\rm{NO}_3^-\), ammonium: \(\rm{NH}_4^+\), phosphate: \(\rm{PO}_4^{3-}\), silicate: \(\rm{SiO}_4^{3-}\)) were low both in the red tide patch and the non-red-tide patch (clear waters outside the red tide patch). Nutrient addition experiments were conducted by adding all the inorganic nutrients to water samples from the two patches followed by incubation for 9 days. The results showed that the addition of inorganic nutrients did not sustain high M. rubrum cell density, which collapsed after day 1, and did not drive M. rubrum in the non-red-tide patch sample to the same high-cell density in the red tide patch sample. This confirmed that nutrients were not the driving factor for the formation of this red tide event, or for its collapse. The death of M. rubrum after day 1 released high concentrations of \(\rm{NO}_3^-\), \(\rm{PO}_4^{3-}\), \(\rm{SiO}_4^{3-}\), \(\rm{NH}_4^+\), and urea. Bacterial abundance and heterotrophic activity increased, reaching the highest on day 3 or 4, and decreased as cell density of M. rubrum declined. The released nutrients stimulated growth of diatoms, such as Chaetoceros affinis var. circinalis, Thalassiothrix frauenfeldii, and Nitzschia sp., particularly with additions of \(\rm{SiO}_4^{3-}\) treatments, and other species. These results demonstrated that initiation of M. rubrum red tides in the bay was not directly driven by nutrients. However, the massive inorganic nutrients released from the collapse of the red tide could induce a second bloom in low-ambient nutrient water, influencing phytoplankton species composition.     

高温干旱背景下太湖藻情变化特征及机制

2022年我国长江流域经历了长期的高温干旱,对湖泊水生态环境和湖内藻情态势产生了深远影响。但目前关于干旱环境下湖泊水华的响应特征研究较少。以太湖为例,基于2005—2022年湖体营养盐与叶绿素a浓度的长期监测数据,结合卫星遥感影像反演的蓝藻水华面积变化,探讨了2022年高温干旱对太湖蓝藻的影响特征及驱动机制。结果表明,2022年蓝藻水华高发季节(5—9月),太湖蓝藻水华的平均面积和最大面积均明显下降,其中5月的水华面积仅为近5年同期平均面积的20%;水样采集分析获得的水体叶绿素a浓度和微囊藻生物量在春季也明显下降。营养盐方面,2022年太湖的总氮和总磷均值分别为1.41和0.084 mg/L,较近5年均值分别下降了30.6%和27.3%,均为2005年以来的最低值。氮磷浓度空间分布的克里金插值显示,除西北湖区(竺山湾)受河流入湖影响外,大部分湖区的溶解态氮磷也都处于较低状态,冬季溶解性总磷浓度小于0.02 mg/L的水域面积占全湖面积的79%。随机森林分析表明,总磷、水温和风速是影响春季微囊藻和藻类生物量的关键因子。冬季湖体磷水平低,加上春季外源负荷较少,致使2022年春季太湖大范围湖...     

太湖近代沉积物中重金属元素的累积

利用210Pb、137Cs定年技术,对来自太湖不同生态和沉积特征的三个湖区的沉积物柱状样品进行了定年,用ICP—AES分析了沉积物中重金属等元素的含量,分析了太湖沉积物中重金属的累积特征及其成因.污染较重、蓝藻水华暴发频繁的梅梁湾沉积物中的重金属含量在近25年来逐年增加;太湖上游风浪较大的夹浦湖区表层10cm沉积速率大、粒度粗,除表层1cm外,1—10cm沉积物中各种重金属含量都较低,且层间变化剧烈;下游湖区正逐渐草型化的胥口湾除表层3cm外,沉积物中重金属的含量自底层向表层大致呈不断下降的趋势.研究表明,不同年代的太湖沉积物中重金属含量差异很大,明显大于不同湖区间沉积物重金属平均含量间的差异.水动力作用引起的沉积物粒度分异很可能是影响沉积物中重金属积累的一个重要因素.总体上太湖沉积物中重金属的污染比较轻微,但已经有一定程度的Cd污染,梅梁湾沉积物中自上世纪70年代开始明显积累Cd,其他重金属元素的积累也逐渐增加,值得关注.     

Discovery of bacteriopheophytin-<Emphasis Type="Italic">a</Emphasis> in lacustrine deposits from Lake Zigetang on the central Tibetan Plateau and its paleoenvironmental significance

Lake Zigetang is located on the central Tibetan Plateau (TP) and represents a rare but typical meromictic lake in China. The lake’s stable meromixis sustains microflora communities, and changes in these communities are relatively independent of climate. Therefore, these communities can be used as paleoclimate proxies. In this paper, the stratification properties and their relationships with the microflora of Lake Zigetang were analyzed. We found that water depth and climate conditions were two important factors for maintaining meromixis in Lake Zigetang. Generally, stratification was enhanced during warm periods, while temperature differences between the mixolimnion and monimolimnion were decreased during cold periods. The presence of anoxygenic phototrophic bacteria (APB) was demonstrated by the discovery of bacteriopheophytin-a (Bph-a) in the sediments. This bacterial community is mainly concentrated at the bottom of the chemocline and the top of the monimolimnion, where it forms a thin APB layer. Moreover, total APB productivity is mainly affected by the light intensity penetrating to the APB layer, which exponentially increases as the thermocline becomes shallow. Therefore, high Bph-a values in the lake corresponded to a shallow thermocline and warm periods, low Bph-a values corresponded to cold periods, and zero changes indicated that the water was completely mixed and reflected an extreme cold climate or low lake level period. Thus, Bph-a can be used as a climate proxy to reconstruct the history of lake stratification and climate changes.     

太湖浮游细菌种群基因多样性及其季节变化规律

为认识湖泊中浮游细菌的多样性及其变化规律,本研究采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)的方法,研究了太湖梅梁湾和湖心区浮游细菌种群16S rRNA基因多样性以及种群基因组成的季节变化特征.研究结果表明,太湖浮游细菌种群具有丰富的基因多样性.浮游细菌的丰度、DGGE条带数、种群的Shannon-Wiener指数、Simpson 指数和Pielou指数均呈现显著的季节变化规律:夏季及秋季浮游细菌的丰度、基因多样性及种群的均匀度较高,而冬季和春季则较低.浮游细菌种群的基因组成随时间变化.梅梁湾浮游细菌的丰度显著高于湖心.梅梁湾与湖心浮游细菌主要建群种的基因型存在显著差异.