光及补料条件对室内管道光反应器中三角褐指藻生长和岩藻黄素积累的影响

【目的】制备富含岩藻黄素的三角褐指藻(Phaeodactylum tricornutum)生物质。【方法】采用470 L室内管道光生物反应器系统,研究自养培养下光源、调光策略、补料培养操作条件对于细胞生长、生物量浓度和岩藻黄素积累的影响。【结果】在采用LED灯、梯度提升照度及重复补料半连续培养模式下,三角褐指藻细胞密度、生物量和岩藻黄素产率最高分别可达3.16×10⁷mL^-1、1.64 g/L和1.90 mg/(L·d),分别是日光灯为光源条件下的2.77倍、3.09倍和2.38倍(P<0.01)。【结论】在管道光生物反应器中获得的自养培养条件,可强化三角褐指藻生物量和岩藻黄素积累,为三角褐指藻积累岩藻黄素的室内可控规模化技术开发提供指导。     

Comparison of Carbon,Nitrogen, and Sulfur in Coastal Wetlands Dominated by Native and Invasive Plants in the Yancheng National Nature Reserve,China

The rapid invasion of the plant Spartina alterniflora in coastal wetland areas can threaten the capacity of their soils to store carbon(C), nitrogen(N), and sulfur(S). In this study, we investigated the spatial and temporal distribution patterns of C, N and S of both soil and(native and invasive) plants in four typical coastal wetlands in the core area of the Yancheng National Nature Reserve, China. The results show that the invasive S. alterniflora greatly influenced soil properties and increased soil C, N and S storage capacity: the stock(mean ± standard error) of soil organic carbon(SOC,(3.56 ± 0.36) kg/m~3), total nitrogen(TN,(0.43 ± 0.02) kg/m~3), and total sulfur(TS,(0.69 ± 0.11) kg/m~3) in the S. alterniflora marsh exceeded those in the adjacent bare mudflat, Suaeda salsa marsh, and Phragmites australis marsh. Because of its greater biomass, plant C((1193.7 ± 133.6) g/m~2), N((18.8 ± 2.4) g/m~2), and S((9.4 ± 1.5) g/m~2) storage of S. alterniflora was also larger than those of co-occurring native plants. More biogenic elements circulated in the soil-plant system of the S. alterniflora marsh, and their spatial and temporal distribution patterns were also changed by the S. alterniflora invasion. Soil properties changed by S. alterniflora's invasion thereby indirectly affected the accumulation of soil C, N and S in this wetland ecosystem. The SOC, TN, and TS contents were positively correlated with soil electrical conductivity and moisture, but negatively correlated with the pH and bulk density of soil. Together, these results indicate that S. alterniflora invasion altered ecosystem processes, resulted in changes in net primary production and litter decomposition, and increased the soil C, N and S storage capacity in the invaded ecosystems in comparison to those with native tallgrass communities in the coastal wetlands of East China.     

服务大局 依法治矿 努力实现矿产资源的合理开发 利用和有效保护

１９９８年是极不寻常的一年。全省各级地矿部门在省委、省政府和国土资源部的正确领导下,以邓小平理论和党的十五大精神为指针,发扬伟大抗洪精神,深入贯彻实施《矿产资源法》及其配套法规,全面推进地矿行政四项政府职能,主动进位,服务全省经济建设,取得了显著成绩...     

Livelihood changes and evolution of upland ethnic communities driven by tourism: a case study in Guizhou Province,southwest China

As an effective livelihood approach to alleviate poverty without rural population migration, ethnic tourism has become the primary choice of economic development in ethnic areas worldwide in addition to traditional livelihood approaches. This article applies the theories of livelihood to study the community evolution driven by tourism livelihood and examine three mountainous tourism communities in different stages of tourist area life cycle. Drawing on the methods of GIS spatial analysis, semi-structured interviews and questionnaires, this article proposes a sustainable livelihood framework for ethnic tourism to explore the evolution of ethnic tourism communities by identifying changes in livelihood assets (natural, financial, social, cultural and human capitals) in the process of tourism development. The results show that the development of ethnic tourism has led to changes in the increase of building land, and the diversification of land use functions with a trend of shifting from meeting local villagers' living needs to satisfying tourists, income composition and uneven distribution of tourism income spatially. Ethnic tourism also led to the deterioration of traditional social management structure, collapse of neighboring relationship, the over- commercialization and staged authenticity of ethnic culture, as well as the gradual vanish of agricultural knowledge with a trend of increasing modern business knowledge and higher education. In addition, these changes, involving livelihood assets from natural, economic, human, social and cultural aspects are interrelated and interactive, which form new evolution characters of ethnic community. This study reveals the conflicts over livelihood approaches which have formed new vulnerabilities to impact on sustainable evolution of ethnic communities. This research provides implications for achieving the sustainable development of ethnic communities with the driving force of tourism livelihood.     

Spatial patterns and storage composition of woody debris in a natural secondary forest dominated by Pinus tabulaeformis on Loess Plateau,China

Woody debris(WD) is an important par of natural Pinus tabulaeformis mixed stands, and i affects the forest ecosystem stability and developmen The WD spatial patterns are especially importan structural characteristics that can provide insights into forest dynamics. In this paper, the WD storage WD spatial patterns and WD associations among the main species were examined in the natural secondary forest on Loess Plateau in northwest China. Data were collected in a 1 ha(100 m × 100 m) permanent plot and all the trees with a diameter at breast height o more than 3 cm were measured and stem-mapped Ripley's K functions from the spatial-point-pattern analysis method were used to analyze the spatia distribution and associations. The results showed tha(1) The total storage of WD was 10.73 t/ha, fallen wood was the main source of WD, and the majority diameters were greater than 20 cm, and in intermediate levels of decay;(2) The overall spatia pattern was closely related to the spatial scale, which exhibited an aggregated pattern on a small scale, and a random pattern on a large scale. The spatia patterns of coarse woody debris also gradually transitioned from an aggregated pattern in fine scales to a random pattern in broader spatial scales, which matched the overall spatial pattern. The spatial intensity was gradually decreased with the increasing diameters, and increased with the decomposition classes;(3) The WD of Pinus tabulaeformis species was negatively associated with Betula platyphylla and Populus davidiana on a small scale but positively associated with these species on a large scale. The spatial pattern and interspecies relations were the results of long-term interactions between the natural secondary forest community and the surrounding natural environment. These findings would provide a scientific basis for the sustainable management and protection of natural secondary forest ecosystems on Loess Plateau.     

Spatial patterns and storage composition of woody debris in a natural secondary forest dominated by <Emphasis Type="Italic">Pinus tabulaeformis</Emphasis> on Loess Plateau,China

Woody debris (WD) is an important part of natural Pinus tabulaeformis mixed stands, and it affects the forest ecosystem stability and development. The WD spatial patterns are especially important structural characteristics that can provide insights into forest dynamics. In this paper, the WD storage, WD spatial patterns and WD associations among the main species were examined in the natural secondary forest on Loess Plateau in northwest China. Data were collected in a 1 ha (100 m × 100 m) permanent plot, and all the trees with a diameter at breast height of more than 3 cm were measured and stem-mapped. Ripley’s K functions from the spatial-point-pattern-analysis method were used to analyze the spatial distribution and associations. The results showed that: (1) The total storage of WD was 10.73 t/ha, fallen wood was the main source of WD, and the majority diameters were greater than 20 cm, and in intermediate levels of decay; (2) The overall spatial pattern was closely related to the spatial scale, which exhibited an aggregated pattern on a small scale, and a random pattern on a large scale. The spatial patterns of coarse woody debris also gradually transitioned from an aggregated pattern in fine scales to a random pattern in broader spatial scales, which matched the overall spatial pattern. The spatial intensity was gradually decreased with the increasing diameters, and increased with the decomposition classes; (3) The WD of Pinus tabulaeformis species was negatively associated with Betula platyphylla and Populus davidiana on a small scale but positively associated with these species on a large scale. The spatial pattern and interspecies relations were the results of long-term interactions between the natural secondary forest community and the surrounding natural environment. These findings would provide a scientific basis for the sustainable management and protection of natural secondary forest ecosystems on Loess Plateau.