栉江珧受精过程的细胞学研究

采用阴干、温差刺激诱导栉江珧亲贝排放和解剖亲贝获取精卵,授精后,通过连续取样直接镜检和卡诺液固定、Giemsa染色后观察,以研究栉江珧受精过程的细胞及染色体变化。结果表明,栉江珧成熟或接近成熟的卵,大多停留在第一次成熟分裂的前中期,接触海水后,进一步发育至中期。通常在26℃的海水中,授精后20min和35min,分别排出第一和第二极体。大约在授精后45min,雌雄原核融合,受精卵开始第一次卵裂。研究还表明,氨海水具有十分明显刺激卵内物质运动和促进卵细胞成熟的作用。     

Seasonal changes of reproductive activity and biochemical composition of pen shell <Emphasis Type="Italic">Atrina pectinata</Emphasis> Linnaeus, 1767 in Bohai Sea,China

Seasonal variations in the biochemical composition and the reproductive cycle of pen shell Atrina pectinata in Bohai Sea were investigated from May 2013 to April 2014. Histological analysis indicated that the reproductive cycle of A. pectinata can be divided into two phases, inactive stage and gametogenesis, which were equally and significantly influenced by seawater temperature and food availability. Gametogenesis began in late autumn (October), and completed in June and July. Spawning took place in August, coinciding with the highest water temperature and the richest phytoplankton. The significantly high glycogen content in adductor muscles sustained throughout the late active and ripeness stages, but plummeted during spawning and inactive stages. The protein content in female gonads exhibited a synchronous increase along with oocyte diameter and lipid content, suggesting that the female gonads could accumulate protein and lipid for vitelline in A. pectinata. Furthermore, the RNA/DNA ratio was found to be a useful index to indicate the level of gonad maturation in both males and females. The findings of the present study provided a foundation for the fishery resource administration and the aquaculture development of this species.     

Abnormal Karyotype of Pen Shell ( Atrina pectinata ) During Its Early Embryonic Development in Late Breeding Season

The pen shell, Atrina pectinata, distributes globally. It is one of the most important edible bivalves in east Asian countries. However, there are multiple     

两种江珧雌雄同体及性转换现象

通过对江珧科两物种栉江珧、二色裂江珧的性腺组织学切片观察 ,发现性转换及雌雄同体的个体 ,雌雄滤泡在性腺中分布有两种类型 ,一种为滤泡混合型 ,另一种为滤泡并存型。性变方向有雄性转化为雌性、雌性转化为雄性及两种性别均衡发育等三种类型 ,且以雄性转化为雌性为主。据此推测性变及雌雄同体在江珧科是较普遍存在的现象。     

Seasonal Variation of Environmental Variables and Phytoplankton Community Structure and Their Relationship in Liaodong Bay of Bohai Sea,China

In this paper, systematic studies on the changes in concentrations of the environmental factors and the net-phytoplankton community, and the relationship between them in the Liaodong Bay, Bohai Sea during 2013 are presented. The PCA results showed that higher levels of nutrients and dissolved heavy metals in the river-estuary-bay system were closely related to the river runoff. Since the influences of industrial and anthropogenic activities, the Liaodong Bay coastal areas are facing a huge environmental challenge of nutrients and heavy metal pollution. Net-phytoplankton community structure showed obvious seasonal succession, among which the dominant and (or) key species were the main factors affecting community structure change and stability. Under certain environmental conditions, the dominant species and (or) key species dominated the phytoplankton community structure succession. The Bio-ENV results suggested that the seawater temperature, nutrient, Cu, Pb, Zn, and Cd in Liaodong Bay are important environmental variables that affect the phytoplankton community structure. Anthropogenic activities have significantly contributed to the changes in concentrations of environmental factors and the net-phytoplankton community structure and stability, and the relationship between them.     

The suspended sediment concentration distribution in the Bohai Sea, Yellow Sea and East China Sea

The distribution of the suspended sediment concentration (SSC) in the Bohai Sea, Yellow Sea and East China Sea (BYECS) is studied based on the observed turbidity data and model simulation results. The observed turbidity results show that (i) the highest SSC is found in the coastal areas while in the outer shelf sea areas turbid water is much more difficult to observe, (ii) the surface layer SSC is much lower than the bottom layer SSC and (iii) the winter SSC is higher than the summer SSC. The Regional Ocean Modeling System (ROMS) is used to simulate the SSC distribution in the BYECS. A comparison between the modeled SSC and the observed SSC in the BYECS shows that the modeled SSC can reproduce the principal features of the SSC distribution in the BYECS. The dynamic mechanisms of the sediment erosion and transport processes are studied based on the modeled results. The horizontal distribution of the SSC in the BYECS is mainly determined by the current-wave induced bottom stress and the fine-grain sediment distribution. The current-induced bottom stress is much higher than the wave-induced bottom stress, which means the tidal currents play a more significant role in the sediment resuspension than the wind waves. The vertical mixing strength is studied based on the mixed layer depth and the turbulent kinetic energy distribution in the BYECS. The strong winter time vertical mixing, which is mainly caused by the strong wind stress and surface cooling, leads to high surface layer SSC in winter. High surface layer SSC in summer is restricted in the coastal areas.     

Distribution and phenogenetic diversity of Synechococcus in the Bohai Sea,China

Journal of Oceanology and Limnology - Synechococcus is one of the most abundant picocyanobacteria in marine ecosystem, and the absence of Prochlorococcus would make it indispensable as a primary...     

Wind stress field over the Bohai Sea,the Yellow Sea and the East China Sea

Seasonal and annual with stress fields over the Bohai Sea, the Yellow Sea and the East China Sea were computed from the wind rose data compiled in the Climatic Atlas of Chinese Offshore Areas and North-west Pacific and published by the Ocean Press in 1982. 684 wind roses in 2° latitude by 2° longitude boxes constructed from 278,815 wind reports are involved in the present study. The computations are principally intended as a data source for further research. Some oceanographic consequences are expounded on.     

有棘和无棘两种表型栉江珧同工酶差异的比较

采用垂直板聚丙烯酰胺凝胶电泳对有棘和无棘两种不同表型的栉江珧（Ａｔｒｉｎａ ｐｅｃｔｉｎｔａ）的外套膜组织进行了ＥＳＴ 和ＳＯＤ 同工酶比较研究。结果显示这两种类型的酶谱表达有一定差异,但同时也存在中间类型,表明有棘和无棘的表型差异,尚不能提供它们应划分为两个种的充足证据     

Evaluation and fusion of SST data from MTSAT and TMI in East China Sea, Yellow Sea and Bohai Sea in 2008

Two typical satellite sea surface temperature (SST) datasets, from the Multi-functional Transport Satellite (MTSAT) and Tropical Rainfall Measuring Mission Microwave Imager (TMI), were evaluated for the East China Sea, Yellow Sea, and Bohai Sea throughout 2008. Most monthly-mean availabilities of MTSAT are higher than those of TMI, whereas the seasonal variation of the latter is less than that of the former. The analysis on the one-year data shows that the annual mean availability of MTSAT (61%) is greater than that of TMI (56%). This is mainly because MTSAT is a geostationary satellite, which achieves longer observation than the sun-synchronous TMI. The daily availability of TMI (28%-75%) is more constant than that of MTSAT (9%-93%). The signal of infrared sensors on MTSAT is easily disturbed on cloudy days. In contrast, the TMI microwave sensor can obtain information through clouds. Based on in-situ SSTs, the SST accuracy of TMI is superior to that of MTSAT. In 2008, the root mean square (RMS) error of TMI and MTSAT were 0.77 K and 0.84 K, respectively. The annual mean biases were 0.14 K (TMI) and -0.31 K (MTSAT). To attain a high availability of SSTs, we propose a fusion method to merge both SSTs. The annual mean availability of fusion SSTs increases 17% compared to MTSAT. In addition, the availabilities of the fusion SSTs become more constant. The annual mean RMS and bias of fusion SSTs (0.78 K and -0.06 K, respectively) are better than those of MTSAT (0.84 K and -0.31 K).     

Changes in nitrogen and phosphorus and their effects on phytoplankton in the Bohai Sea

Systematic studies of the changes in dissolved inorganic nitrogen (DIN) and dissolved inorganic phosphorus (DIP) and their effects on phytoplankton over the last 30 years in the Bohai Sea are presented. The amount of sewage disposal, use of fertilizer and the Huanghe River runoff were found to have a significant influence on the DIN or DIP concentrations in the Bohai Sea over the last 30 years. Moreover, the changes in DIN and DIP resulted in changes in the limiting nutrients of phytoplankton in the Bohai Sea from nitrogen in the early 1980s to nitrogen-phosphorus in the late 1980s, and then to phosphorus after the 1990s. In addition, changes in nitrogen and phosphorus had a significant effect on the phytoplankton community structure. The half saturation constant (K _s) was used to evaluate the effect of nutrients on the phytoplankton community structure in the Bohai Sea over the last 30 years. Cell abundance percentages of dominant phytoplankton species with high K _s values for phosphorus and low K _s values for nitrogen have decreased since the 1980s, while those of dominant phytoplankton species with low K _s values for phosphorus and high K _s values for nitrogen increased during this period.     

Biochemical Composition and Nutritional Value of Different Shell Color Strains of Pacific Oyster Crassostrea gigas

The shell color of Pacific oyster(Crassostrea gigas) is a desirable trait; but the nutritional studies on C. gigas with different shell colors have not been conducted. Through successive selective breeding, five shell color strains of black(B), purple(P), orange(O), golden(G) and white(W) C. gigas have been developed. The aim of this study was to evaluate the chemical composition and nutritional value of five shell color strains and one commercial population with a common color. The biochemical composition including moisture, total protein, glycogen, ash, total fat, fatty acids(FA), amino acids and minerals was detected. The results indicated that the protein(50.76%–56.57%) was the major component. The content of glycogen showed a significant difference between orange shell and golden shell strains, as well as between commercial population and golden shell strain. In addition, all shell color strains contained a large amount of essential amino acids(12.20–14.15 g(100 g)~(-1)), of them leucine(2.81–3.29 g(100 g)~(-1)) and lysine(2.79–3.28 g(100 g)~(-1)) were predominant. The oysters were rich in polyunsaturated fatty acids(42.26%–45.24% of total fatty acid) with high levels of DHA(18.53%–21.16% of total fatty acid) and EPA(17.23%–18.68% of total fatty acid). Significant differences of mineral contents(Mg, Zn, Fe and Cu) were identified among the six populations. These results indicated that C. gigas with different shell colors presented rich nutritional value with high protein, glycogen, essential amino acids and polyunsaturated fatty acids. The biochemical composition obtained in this study is useful for selective breeding of C. gigas with different shell colors.     

Distribution of nutrients and eutrophication assessment in the Bohai Sea of China

Water samples were collected in 120 stations in the Bohai Sea of China to analyze the distribution of dissolved nutrients and assess the degree of eutrophication in August 2002. The result shows that the average concentration of DIN increased and the PO4-P concentration sharply decreased compared to the previous data of corresponding period. The high concentrations of DIN and PO4-P occurred in coastal waters, especially in the bays and some river estuaries, while the high concentrations of SiO3-Si in the surface and middle depth occurred in the central area of the Bohai Sea. The average ratio of DIN/ PO4-P was much higher than the Redfield Ratio (16:1). Apparently, PO4-P was one of the limiting nutrient for phytoplankton growing in the sea. The average concentrations of DON and DOP were higher than their inorganic forms. The results of eutrophication assessment show that 22.1% of all stations were classified as violating the concentration levels of the National Seawater Quality Standard (GB 3097-1997) for DIN and only 3.9% for PO4-P. The average eutrophication index in the overall area was 0.21±0.22 and the high values occurred in Bohai Bay, Liaodong Bay and near the Yellow River estuary. This means that the state of eutrophication was generally mesotrophic in the Bohai Sea, but relatively worse in the bays, especially some river estuaries.     

Seasonal community structure of mesozooplankton in the Daya Bay, South China Sea

Mesozooplankton are key components of coastal ecosystems, linking the microbial food web to the classic food chain. In this study, species composition and abundance of mesozooplankton is studied for the Daya Bay in April (spring) and October (fall), 2006. A total of 27 species of mesozooplankton were identified in spring and 58 species in fall. Dominant species were Oithona tenuis, Flaccisagitta enflata, Penilia avirostris and Centropages tenuiremis in spring, shifting to Microsetella norvegica, Oithona tenuis and Parvocalanus crassirostris in fall. Higher mesozooplankton abundance was found at Aotou Cove and Dapeng’ao Cove compared to other stations, indicating the influence of eutrophication on mesozooplankton community in the Daya Bay. The outbreak of Noctiluca scintillans bloom in spring reduced the species diversity and abundance of mesozooplankton.     

Phytoplankton Species Composition of Four Ecological Provinces in Yellow Sea,China

The ecological province based on phytoplankton species composition is important to understanding the interplay between environmental parameters and phytoplankton species composition. The aim of this study was to establish phytoplankton species composition ecological pattern thus elucidate the relationship between environmental factors and the phytoplankton species composition in the ecological provinces. Phytoplankton samples were collected from 31 stations in Yellow Sea(121.00?–125.00?E, 32.00?–39.22?N) in November 2014. The samples were enumerated and identified with the Uterm?hl method under an optical inverted microscope-AE2000 with magnifications of 200 × or 400 ×. In the present study, a total of 141 taxa belonging to 60 genera of 4 phyla of phytoplankton were identified, among them 101 species of 45 genera were Bacillariophyta, 36 species of 11 genera were Dinophyta, 3 species of 3 genera were Chrysophyta and 1 species of 1 genera was Chlorophyta. The study area was divided into 4 ecological provinces according to an unsupervised cluster algorithm applied to the phytoplankton biomass. A T-S(Temperature-Salinity) scatter diagram depicted with data of water temperature and salinity defined by environmental provinces matched well with the ecological provinces. The results of Canonical Correspondence Analysis(CCA) indicated that the phytoplankton species composition was mainly correlated with temperature, salinity and silicate concentration in the studied area. A method of establishing ecological provinces is useful to further understanding the environmental effects on the marine phytoplankton species composition and the consequent marine biogeochemistry.     

Seasonal Variation of Biochemical Components in Clam(Saxidomus purpuratus Sowerby 1852) in Relation to Its Reproductive Cycle and the Environmental Condition of Sanggou Bay,China

Seasonal variation of biochemical components in clam(Saxidomus purpuratus Sowerby 1852) was investigated from March 2012 to February 2013 in relation to environmental condition of Sanggou Bay and the reproductive cycle of clam.According to the histological analysis,the reproductive cycle of S.purpuratus includes two distinctive phases:a total spent and inactive stage from November to January,and a gametogenesis stage,including ripeness and spawning,during the rest of the year.Gametes were generated at a low temperature(2.1℃) in February.Spawning took place once a year from June to October.The massive spawning occurred in August when the highest water temperature and chlorophyll a level could be observed.The key biochemical components(glycogen,protein and lipid) in five tissues(gonad,foot,mantle,siphon and adductor muscle) were analyzed.The glycogen content was high before gametogenesis,and decreased significantly during the gonad development in the gonad,mantle and foot of both females and males,suggesting that glycogen was an important energy source for gonad development.The protein and lipid contents increased in the ovary during the gonad development,demonstrating that they are the major organic components of oocytes.The lipid and protein contents decreased in the testis,implying that they can provide energy and material for spermatogenesis.The results also showed that protein stored in the mantle and foot could support the reproduction after the glycogen was depleted.