The Antitumor Components from Marine-derived Bacterium Streptoverticillium luteoverticillatum 11014 Ⅱ

Eight known compounds were isolated from a marine-derived bacterium Streptoverticillium luteoverticillatum 11014 using bioassay-guided fractionations. Their structures were identified by spectral analysis as bis （4-hydroxybenzyl） ether （1）, p-hydroxyphenylethyl alcohol （2）, N-（4-hydroxyphenethyl） acetamide （3）, indole-3 carboxylic acid methyl ester （4）, dibenzo[b,e] [1,4]dioxine （5）, thymine （6）, cytosine deoxyribonucleoside （7） and 2, 3-butanediol （8）. These compounds were evaluated for their cytotoxic activities against K562 cell line with the SRB method for the first time. Compounds 2 and 4 showed cytotoxcities with IC50 values of 101.1 and 165.3 μolL^-1, respectively. All compounds were isolated from S. luteoverticillatum 11014 for the first time.     

The influence of Fe^2＋ on growth and development of cells enzymatically isolated from Porphyra yezoensis blades

Fe^2＋ acted as an accessorial factor for many cellular enzymatic reactions is very important for seaweed growth and development, but the Fe^2＋ requirement in nori had not been seen. Porphyra yezoensis cells were separated enzymatically and cultured in a series of sterilized seawater media containing various concentrations of Fe^2＋. The growth development and cell were investigated in this work. Through this experiment, two biologically-meant concentration scales were found, one is low concentrations, 12.1-102.1μg/L, 10-100 times than that in seawater, favoring the development of isolated cells of Porphyra and the other was high concentrations, more than 10mg/L inhibiting the cell growth, leading to the deformity and shrinkage of the cells. At the concentration of 50 mg/L, the cells stopped growing and died eventually.     

网板Ⅱ

(五)立式网板立式网板平面近于矩形,其特点是展弦比大,网板宽度大,象矩形网板竖立起来一样,故称立式网板,网板剖面基本上为两种类型,一类是机翼剖面(图9),因此也叫翼型网板,另一类为弧形剖面,其中还可分为圆弧(图10)与非圆弧(图11)两种。立式网板全板     

The influence of Fe~(2+) on growth and development of cells enzy-matically isolated from Porphyra yezoensis blades

1 INTRODUCTION Porphyra (also called nori in Japanese) is one of the most important algae in aquaculture in the world. In the early 1980’s, basic studies on cell and protoplast isolation, cultivation and “seedling” breeding of Porphyra were conducted in laboratory. Zhao and Zhang (1981) isolated and cultured isolated cells by grinding the nori thallus, and Lu (1983) isolated cells by digesting bacteria. An enzyme isolated from a sea snail, was used to isolate cells and protoplasts (T…     

The substance composition of sterols in the sediments from the Chukchi Sea, the Bering Sea and global climatic significance

The compounds of sterols such as C27 ,C2s ,C29 and C30 are recorded from C-8 core of the Chukchi Sea. The double bond position is located at 5-, 5,22 as well as 22-,24-. The compound of sterols such as C27 ,C28 ,C29 are recorded from B2-9 core of the Bering Sea. The double bond position is located at 5-, 5, 22 as well as 22. The composition characteristics of sterols indicate that the substance is mainly contributed by the terrigenous origin and marine silicate organisms. The results are also suggest that the record of abnormal sterols from the surface sediments （2 -0 era）in the Chukchi Sea and the Bering Sea represent the period from 1980s to the late 1990s. The strong signal of the Arctic warming is preserved in the sediments, which indicates the eco - environmental change responding to climatic effect of circumjacent.     

Geochemical Characteristics and Sources of Some Chemical Components in Sediments of Zhuiiang（Pearl） River Estuary

Sediment cores with length of 25 - 45cm at 12 stations in Zhujiang(Pearl) River Estuary were sampled in summer of 1999. Chemical components (Al2O3 , Fe2O3, CaO, TiO2, Cu, Pb, Cr, Zn, Co, Ni, V, Ba, Sr and Y) and grain sizes were analyzed. Sediments in Zhujiang River Estuary are characterized by relatively coarse grain size. The grain sizes of the sediments in the area outside the estuary are relatively finer than those in the north part of the estuary but coarser than those in the middle of Lingdingyang and have a trend similar to the spatial distribution trend of some chemical components such as Fe2O3, Al2O3, TiO2, V, Cu, Ni, Zn and Y. This showed that grain size is an important factor that controls the spatial distribution and depth profile of chemical elements in the Zhujiang River Estuary. Frequent re-suspension, re-transportation and redistribution of settled sediment on estuary floor could be deduced from the spatial distributions and depth profiles of chemical components. Except for CaO and Sr, the chemical components always enrich at the west side of, in the middle of, and at the north side of, the estuary. Fe2O3, Al2O3, Ti, V, Cr, Ni, Zn, Cu and Y in Zhujiang River Estuary sediments mainly result from rock and soil weathering except that parts of Cr, Ni, Zn Cu and Y are anthropogenic. CaO and Sr in sediments originate from marine source. Although Co as well as Pb derives from anthropogenic sources, human activities related to Co might differ from the activities related to Pb.     

The first dinosaur tracksite from Xinjiang, NW China （Middle Jurassic Sanjianfang Formation, Turpan Basin） a preliminary report

A new dinosaur tracksite was discovered in a steeply inclined sandstone layer of the Middle Jurassic Sanjianfang Formation in the Shanshan area of the Turpan Basin. The site is the first record of dinosaur footprints from Xinjiang Province in northwestern China. More than 150 tridactyl theropod dinosaur footprints are preserved as positive hyporeliefs on the lower bedding plane of a fine-grained sandstone body. Most of the footprints are isolated and appear to be randomly distributed. Some show well defined phalangeal pads, heels and rarely indistinct impressions of the distal part of the metatarsus. Two distinct morphotypes are present: a larger type with relatively broad pads shows similarities to Changpeipus and Megalosauripus, and a slightly smaller, slender and gracile type which is similar to Grallator, Eubrontes and Anchisauripus. In both morphotypes, digit III is the longest with a length between 11.4 and 33.6 cm. A single imprint shows prominent scratches, probably formed during slipping of the track maker.     

The Complete Sequence of Mitochondrial COⅡ Gene of Fenneropenaeus chinensis and Its Applicability as a Marker for Phylogenetic Analysis

The complete mitochondrial cytochrome oxidase subunit Ⅱ (COⅡ) gene of Penaeinae shrimp Fenneropenaeus chinensis was cloned and sequenced. The gene is 688 bp in length and codes for 229 amino acids. It shows 83.2%, 87.0% and 83.8% sequence similarity to Marsupenaeus Japonicus, Penaeus monodon and Farfantepenaeus notialis, respectively. The A+T content of the whole gene and that at the third position of codons are 64.7% and 78.2%, respectively. The phylogenetic relationship between F. chinensis and three other species representing genera Farfanatepenaeus, Marsupenaeus and Penaeus was analyzed. Results showed that the genetic distances among the four taxa ranged from 0.144 0 to 0.200 5, exceeding those estimated with COⅠ and partial 16S rRNA gene sequences among Marsupenaeus, Litopenaeus and Melicertus, and being therefore larger than the value among subgenera. It has been suggested that the COⅡ gene has a faster evolutionary rate than that of the COⅠ gene and partial 16S rRNA gene and could be used for phylogenetic analysis at genus or species level. The results of the present study indicated that Farfantepenaeus, Fenneropenaeus, Marsupenaeus and Penaeus are at a higher phylogenetic level than subgenus, which supports the opinion of the elevation of phylogenetic status of the four subgenera to genus level.     

The Complete Sequence of Mitochondrial COⅡ Gene of Fenneropenaeus chinensis and Its Applicability as a Marker for Phylogenetic Analysis

The complete mitochondrial cytochrome oxidase subunit Ⅱ （COⅡ） gene of Penaeinae shrimp Fenneropenaeus chinensis was cloned and sequenced. The gene is 688 bp in length and codes for 229 amino acids. It shows 83.2%, 87.0% and 83.8% sequence similarity to Marsupenaeus Japonicus, Penaeus monodon and Farfantepenaeus notialis, respectively. The A＋T content of the whole gene and that at the third position of codons are 64.7% and 78.2%, respectively. The phylogenetic relationship between F. chinensis and three other species representing genera Farfanatepenaeus, Marsupenaeus and Penaeus was analyzed. Results showed that the genetic distances among the four taxa ranged from 0.144 0 to 0.200 5, exceeding those estimated with COⅠ and partial 16S rRNA gene sequences among Marsupenaeus, Litopenaeus and Melicertus, and being therefore larger than the value among subgenera. It has been suggested that the COⅡ gene has a faster evolutionary rate than that of the COⅠ gene and partial 16S rRNA gene and could be used for phylogenetic analysis at genus or species level. The results of the present study indicated that Farfantepenaeus, Fenneropenaeus, Marsupenaeus and Penaeus are at a higher phylogenetic level than subgenus, which supports the opinion of the elevation of phylogenetic status of the four subgenera to genus level.     

Snow water content estimation from measured snow temperature

The vertical temperature profiles of snow and sea ice have been measured in the Arctic during the 2nd Chinese National Arctic Research Expedition in 2003(CHINARE2003).The high-resolution temperature profile in snow is solved by one-dimensional heat transfer equation.The effective heat diffusivity,internal heat sources are identified.The internal heat source refers to the penetrated solar radiation which usually warms the lower part of the snow layer in summer.By temperature gradient analysis,the zero level can be clarified quantitatively as the boundary of the dry and wet snow.According to the in situ time series of vertical temperature profile,the time series of water content in snow is obtained based on an evaluation method of snow water content associated with the snow and ice physical parameters.The relationship of snow water content and snow temperature and temporal-spatial distribution of snow water content are presented     

The Latest Research Progress on El Nino

Cold water in the deep Pacific can be drawn up to the surface (or west warm water drifts eastwards) because strong tide increases the mixing of seawater both in vertical and horizontal. In this way greenhouse effect is decreased or in-creased by means of absorbing (or releasing) CO2. Therefore, La Nina cold event (or El Nino warm event) may occur, which is caused by wanning - up or cooling - down air above the ocean. Volcanic action at sea bottom is also controlled by strong tide.     

The Latest Research Progress on El Ninod

1　ＭａｔｈｅｍａｔｉｃＭｏｄｅｌｏｆＴｉｄａｌＶｉｂｒａｔｉｏｎＳｕｐｐｏｓｅｔｈａｔ 3ｓｅｍｉ -ａｘｅｓｏｆａｅｌｌｉｐｓｏｉｄａｒｅａ ,ｂａｎｄｃ (ａ >ｂ >ｃ) ,ｌａｔｉｔｕｄｅｉｓθ ,ｌｏｎｇｉｔｕｄｅｉｓψ(ｃｈａｎｇｉｎｇｆｒｏｍ 0°ｔｏ 90°) ,     

Chemical characterization of fractions of dissolved humic substances from a marginal sea—a case from the Southern Yellow Sea

Marine dissolved organic matter(DOM) is one of the largest dynamic pools of organic carbon in the global carbon cycle, yet DOM is still chemically poorly characterized. To better understand the origin, composition, and cycling of DOM in the China marginal sea, dissolved humic substances(DHS) were isolated from seawaters in two locations in the Southern Yellow Sea. The DHS were subdivided into fulvic acids(FAs), humic acids(HAs) and the XAD-4 fractions. Complementary analytical approaches were used to characterize the isolated DHS samples including stable carbon isotopic composition, Fourier transform infrared spectroscopy(FTIR), ~(13) C cross polarization magic angle spinning(CP/MAS) nuclear magnetic resonance(NMR), and pyrolysis gas chromatography-mass spectrometry(Py-GC/MS). The results demonstrated that both DHS samples encountered the influences from marine source, indicating that algal and microbial-derived materials are the predominant precursors for the studied samples. The three fractions of DHS showed diff erent properties. FAs presented more aromatic features, whereas HAs contained more aliphatic lipids and proteinaceous materials. The XAD-4 fractions were enriched in ~(13) C and contained more carbohydrates but less aromatic compounds. The lower molecular weight and higher heteroatom content and number of carboxyl groups for the XAD-4 fractions may give them considerable geochemical significance for aspects of trace metal species, bioavailability of pollutants, mineral weathering and water acidification in marine environments.     

The Role of Aga Khan Rural Support Programme in Rural Development in the Karakorum, Hindu Kush ＆ Himalayan Region： Examples from the Northern Mountainous Belt of Pakistan

Pakistan is predominantly a mountainous country where rural development activities are characterised by inconsistency, politically motivated short-term projects without proper feedback. Since the inception of the country, the top-down approach has been followed, and the same development plans that were formulated for the plain areas have been extended to the mountains without any modification. In doing so, neither the participation of the local communities was cared for, nor the mountain specificities were considered in the planning process. Moreover, the representation of the local inhabitants was improper and contradictory to the facts. This biased approach has been one of the main causes for the failure of development projects carried out by different agencies of the Government. Contrary to the perception of the state authorities, the mountain communities proved to be more open to accept new approaches and demonstrated the capacity and capability of being a dependable development partner. In this paper, a detailed account of the Aga Khan Rural Support Programme （AKRSP） has been presented to assess and evaluate the approach followed by this non-governmental organisation （NGO）, and the response of the local inhabitants as collaborators in the development process. The achievements of the AKRSP from project planning, implementation and monitoring can be adopted as a model for rural development not only in the plains, but also in the mountainous areas of the developing countries in the world.     

The auxin concentration in sixteen Chinese marine algae

1 INTRODUCTION Auxins are plant hormones. They play impor- tant roles in regulating plant activities, including phototropism, fruit development, root initiation and so on. In early 1940, Thirmann and Scoog (1940) found that auxin was present in more than …     

改进C-dbase Ⅱ中JOIN命令的方法

本文作名给出了对IBM-PC(0520)机。C-dbaseⅡ中JolN命令运用的改进方法,运用该方法,使用两个数(?)岸的连续速度大为加快。     

A new radiolarian species Clathrocorys gracilis from northern South China Sea

A new species, Clathrocorys gracilis nov. spec., of Tripocyrtidae （Radiolarians： Nassellaria） from surface water in northern South China Sea, is described in this paper. The new species has four radial beams arising from the cephalic base centre, each foot with cup-shaped structure and ramified bar in the proximal section （near cephalic base）, and each wing with a large mesh in the middle, a medium mesh and a small mesh on each side respectively.     

Purification and characterization of lectin from humoral fluids of Charybdis feriatus

1 INTRODUCTION Lectins have been regarded as having a puta- tive role in non-self recognition in vertebrate and invertebrate immunities (Arason, 1996; Matsushita, 1996; Olafsen, 1996; Vasta et al., 1999; Wilson, et al., 1999; Marques and Barracco, 2000). …