Spatiotemporal pattern of bacterioplankton in Donghu Lake

Bacterioplankton play key roles in the biogeochemical cycle and in organic contaminant degradation. The species richness and abundance of bacterial subgroups are generally distinct from each other, and this is attributed to their different functions in aquatic ecosystems. The spatiotemporal variations of eight phylogenetic subgroups （Actinobacteria, Bacteroidetes, Cyanobacteria, Firmicutes, Planctomycetes, alpha-, beta-, and gamma-Proteobacteria） derived from Donghu Lake were investigated using PCR-DGGE fingerprinting, to explore their responses to environmental factors. Results indicate that Actinobacteria and beta-Proteobacteria were the two largest bacterial subgroups detected. These two groups and Bacteroidetes showed clear seasonal patterns in composition of the operational taxonomic unit. Results also suggest that the bacterioplankton subgroups in Donghu Lake were significantly correlated with different environmental factors. In brief, the total nitrogen was one of the major factors regulating all the bacterioplankton except for Actinobacteria. However, total phosphorus, another important eutrophication factor, contributed to the two largest bacterial groups （Actinobacteria and beta-Proteobacteria）, as well as to the Cyanobacteria and Firmicutes. Therefore, the responses of bacterioplankton subgroups to environmental factors were different, and this should be attributed to the differences in the fimctions of different groups.     

Effect of enclosure and nutrient enrichment on Microcystis blooms in Donghu Lake

1 INTRODUCTION Surface blooms of nuisance cyanobacteria are often a signal of eutrophication (Jacoby et al., 2000). They are generally favored by conditions of low nitrogen to phosphorus ratios, high total phosphorus content, high water temperature, high …     

Seasonal changes of dissolved and particulate organic carbon in Donghu Lake, China

Concentrations of dissolved and particulate organic carbon (DOC and POC) were documented in 1996–1997 at 4 different trophic state stations in Donghu Lake, a typical shallow eutrophic lake along the Changjiang River's middle reaches. The mean concentrations of DOC were 15.11±3.26, 15.19±4.24, 14.27±3.43, and 13.31±3.30 mg/L in Station I, II, III, and IV, respectively. The DOC concentrations of the studied area were very similar to that in other lakes along the Changjiang River's middle reaches. The POC mean of the whole lake was 5.01 mg/L due to the large amount of organic detritus of both allochthonous and autochthonous origin. Significant linear relationship was found between POC and chlorophyll a at all 4 stations, which presumably reflect that phytoplankton, its exudates and its metabolic products were the main contributors to the POC pool in the water column. The slope of such linear relationship at Station IV was significantly steeper than that at Station I, II and III. In addition, the DOC/POC ratios (mean value: 4.40) indicated that the organic detritus was the most important component of the particulate organic matter; in other works, next to organic detritus, phytoplankton dominated the particulate organic matter in Donghu Lake. Project 39770146, 39430101 supported by NSFC and the State Key Laboratory of Freshwater Ecology and Biotechnology, affiliated to the Institute of Hydrobiology, Chinese Academy of Sciences.     

Effects of hydrological connection and human disturbance on genetic variation of submerged Vallisneria natans populations in four lakes in China

With the increase in the need for flood prevention and lake resource used by humans,the construction of floodgates and sluices has changed the hydrological connection between rivers and lakes,and between adj acent lake s.In river-disconnected lake s,exploitation and use of lake re sources have resulted in water quality decline and mechanical disturbance intensification to a different degree.Of the large number of river-disconnected lakes in the middle-lower reaches of the Changjiang(Yangtze) River,the Futou Lake,and the Xiliang Lake lie close together and are,historically,directly connected,and so do Liangzi Lake and Baoan Lake.The extent of human disturbance is severe in the Futou Lake and the Baoan Lake,but relatively mild in the Xiliang Lake and Liangzi Lake.The freshwater rosette-forming submerged plant Vallisneria natans is one of the dominant species in the four lakes.Using microsatellite markers,we studied the genetic variation of V.natans subpopulations in lakes with different intensities of human disturbance and historical direct hydrological connections.Our results showed that human disturbance decreased plant density and clonal growth in V.natans,but might increase genetic and clonal diversity at a subpopulation level and enhance gene flow among subpopulations by sexual propagule movement.Under similar climatic conditions,different intensities of disturbance seem to have such a high selective potential to differentiate genetically adjacent lake populations that they outperform the forces of gene flow through historical direct hydrological interconnection,which tends to produce genetic homogeneity.Our findings imply that human disturbance has a profound effect on the evolutionary process of natural populations of submerged plants.Moreover,increased subpopulation genetic diversity can enhance resistance and resilience to environmental disturbances.To a certain degree,we could expect that disturbed populations have the possibility of re storing spontaneously if humans cease to perturb natural ecosystems in the future.     

Preliminary studies on the role of protozoa in the food web of Donghu Lake

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Experimental studies on the role of planktivorous fishes in the elimination ofMicrocystis bloom from Donghu Lake using enclosure method

The hypertrophic subtropic Donghu Lake's dense water bloom (of mainlyMicrocystis, Anabaena andOscillatoria) that occurred annually from the beginning of the 1970s, has disappeared since 1985. The influence of planktivorous fishes (silver and bighead carps) on the water bloom was studied for three years using the enclosure method. The enclosures stocked densely with bighead and/or silver carp were free of water bloom during the experimental period. The water bloom that appeared in the fish-free enclosures was completely eliminated in 10–20 days by introduction of silver and/or bighead carp(grass carp was not effective in controlling water bloom). This study showed clearly that grazing pressure by planktivorous fishes is a key factor in eliminating water bloom from the lake. This work was supported by FEBL (State Key Laboratory for Freshwater Ecology and Biotechnology of China) funds and by the National Natural Science Foundation of China (Grant No., 3937014).     

Epiphytic bacterial communities on two common submerged macrophytes in Taihu Lake: diversity and host-specificity

Leaves of terrestrial and aquatic plants are home to a wide diversity of bacterial species. However, the diversity and variability of epiphytic bacteria on their submerged plant hosts remains poorly understood. We investigated the diversity and composition of epiphytic bacteria from two common submerged macrophytes: Vallisneria natans and Hydrilla verticillata in Taihu Lake, Jiangsu, China, using methods of terminal restriction fragment length polymorphisms (T-RFLP) and clone library analyses targeted at bacterial 16S rRNA genes. The results show that: (1) the libraries of the two waterweeds contain wide phylogenetic distribution of bacteria, and that the sequences of the two libraries can be separated into 93 OTUs (at 97% similar value); (2) Betaproteobacteria, including Burkholderiales, was the most abundant bacterial group on both plants. Cyanobacteria and Gammaproteobacteria were the second largest groups on V. natans and H. verticillata, respectively. Both clone libraries included some sequences related to those of methanotrophs and nitrogen-fixing bacteria; (3) Cluster analysis of the T-RFLP profiles showed two distinct clusters corresponding to the two plant populations. Both ANOSIM of the T-RFLP data and Libshuff analysis of the two clone libraries indicated a significant difference in epiphytic bacterial communities between the two plants. Therefore, the epiphytic bacterial communities on submerged macrophytes appear to be diverse and host-specific, which may aid in understanding the ecological functions of submerged macrophytes in general.     

Species composition and seasonal density dynamics of ciliated Protozoa in Baoan Lake with particular reference to the submerged macrophytes

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Temporal and spatial variation of fish assemblages in Dianshan Lake,Shanghai, China

Using multi-mesh gillnets and trawls, the fish communities in Dianshan Lake at 6 stations from Oct. 2009 to Jul. 2010 were investigated seasonally to reveal the biodiversity and its spatial and temporal distribution patterns. The long-term changes in their structural characteristics were then analyzed to identify the main infl uencing factors and several measures for lake restoration were put forward. Thirty six species, belonging to 9 family and 30 genera, were collected, amongst which, the order Cypriniformes accounted for 61.1% of the total species number. In terms of importance value, Cypriniformes was the predominant group, Coilia nasus the dominant species, while Cyprinus carpio and Rhinogobius giurinus were the subdominant taxa. The community types did not differ among stations, but between seasons. There were no significant differences between seasons and among stations in species diversity, but richness differed both spatially and seasonally. Along with the process of eutrophication and the drastic reduction of the area colonized by macrophytes from 1959 to 2009–2010, the fish diversity declined markedly, and species numbers of herbivores and piscivores declined proportionately more than those of invertivores, omnivores, and planktivores. The decline of potamophilus and river-lake migratory fish was more marked than those of sedentary, river-sea migratory, and estuarine fishes. Eutrophication concomitant with sharp reduction of macrophyte area and overfishing may be the main reasons for the decline in fish diversity in Dianshan Lake.     

Degradation of linear alkylabenzene sulfonate (LAS) and its compounds in Donghu Lake (Hubei,P.R.C.) determined by high performance liquid chromatography (HPLC)

Commercial linear alkylbenzene sulfonate (LAS), mixture of alkylchain lengths and phenyl position isomers (C₁₀−C₁₃), is widely used as a major constituent of household and industrial detergents in the People's Republic of China. Degradation process and behaviour of LAS compounds during an 82-hour lake water die-away study, with an added LAS concentration of 1.5mg·L⁻¹, was quantified and accomplished by HPLO-UV after extractionon the SepPek C₁₈ reversed-phase cartridges. The degradation rate became progressively faster with increasing chain length. The technique described in this study is fast, sensitive and specific, and can be used to determine low levels of LAS and for establishing water quality criteria and standards relating to LAS and its compounds. Dr. Ayfer Yediler (GSF, National Center for Envirommental Sciences, Ingolstaedter Landstr. 1, D-8042 Neuherberg, FRG) is guest scientist in the Institute of Hydrobiology, Academia. Sinica, Wuhan.     

Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803

Journal of Oceanology and Limnology - Synechocystis sp. PCC 6803 is a model organism widely used in cyanobacterium biology and biotechnology. To know the genetic background of substrains of...     

可可西里苟仁错湖泊沉积物元素地球化学特征及其环境意义

湖泊沉积物元素地球化学特征及其比值，有效地揭示了湖泊沉积物物源区的化学风化程度．已成为反映古气候波动的有效指标。根据可可西里苟仁错湖泊沉积物的元素地球化学特征，通过CIA，ICV等元素比值的结果，揭示该地区1400AD以来化学风化作用十分微弱，因此湖泊沉积物元素组成，对气候变化非常敏感。研究表明1400AD以来该地区气候经历多次冷暖波动，且呈干旱化的趋势，1420AD左右进入小冰期，其中1480～1520AD最为寒冷，1900AD后干旱化加剧。     

Contrasting patterns of free-living bacterioplankton diversity in macrophyte-dominated versus phytoplankton blooming regimes in Dianchi Lake,a shallow lake in China

Freshwater shallow lakes typically exhibit two alternative stable states under certain nutrient loadings:macrophyte-dominated and phytoplankton-dominated water regimes.An ecosystem regime shift from macrophytes to phytoplankton blooming typically reduces the number of species of invertebrates and fishes and results in the homogenization of communities in freshwater lakes.We investigated how microbial biodiversity has responded to a shift of the ecosystem regime in Dianchi Lake,which was previously fully covered with submerged macrophytes but currently harbors both ecological states.We observed marked divergence in the diversity and community composition of bacterioplankton between the two regimes.Although species richness,estimated as the number of operational taxonomic units and phylogenetic diversity(PD),was higher in the phytoplankton dominated ecosystem after this shift,the dissimilarity of bacterioplankton community across space decreased.This decrease in beta diversity was accompanied by loss of planktonic bacteria unique to the macrophyte-dominated ecosystem.Mantel tests between bacterioplankton community distances and Euclidian distance of environmental parameters indicated that this reduced bacterial community differentiation primarily reflected the loss of environmental niches,particularly in the macrophyte regime.The loss of this small-scale heterogeneity in bacterial communities should be considered when assessing long-term biodiversity changes in response to ecosystem regime conversions in freshwater lakes.     

2011年洪湖和梁子湖受旱程度差异和成因分析

湿地是水陆交错的生态系统,往往受旱灾影响大,故此,对不同湖泊湿地受旱程度差异及其原因分析,可为减灾和湿地保护提供科学依据。本文利用2010年5月和2011年5月2个时相的HJ-1卫星CCD影像,提取洪湖和梁子湖2个时相的湿地水面积,结合统计资料和问卷调查数据,从湿地水面积及空间分布变化、湿地生态系统功能退化、湿地生态系统,对人类福祉影响3方面,分析了两湖湿地受旱程度的差异。并综合气象数据、统计资料和问卷调查数据,从气象因子、区域自然地理环境、湿地类型及特点、区域水文条件和湿地受保护程度5方面探讨两湖受旱程度差异的原因。结果表明：（1）2011年春旱期间,洪湖比梁子湖受旱程度更严重。旱灾时,洪湖水面积减少83.93 km2,减小比例为26%,梁子湖水面积减少13.02 km2,减小比例为4%;两湖的涵养水源、保护生物多样性、物质生产和水质净化功能均受到损害,洪湖较梁子湖更严重;受旱灾影响,洪湖湿地对人类福祉的消极影响大于梁子湖。（2）降雨量、气温和风速等气象因素、湖泊湿地成因类型、区域自然地理环境和水文条件是影响两湖受旱程度差异的主要因素,另外,湿地保护措施的差异及效果也会造成两湖受旱程度不同。     

滇池湖滨带植被时空变化及其对滇池总磷浓度的影响

滇池是中国最大的高原淡水湖泊,是长江上游生态安全格局的重要组成部分。自2008年起政府在滇池湖滨带大规模退耕还草还林,以恢复滇池及其湖滨生态。湖滨带植被对滇池总磷浓度的影响是一个长期过程,需要通过遥感长时间连续监测的数据来评价。本文以滇池和滇池湖滨带为研究区,从空间分布、季节差异2个角度,结合入湖河流总磷负荷,研究2005—2018年滇池湖滨植被时空变化对滇池总磷浓度的影响。主要结论如下：① 滇池总磷浓度由湖缘向湖心逐渐降低,并且北部和南部偏高;2005—2018年,滇池总磷浓度呈现显著下降趋势;② 2011—2018年滇池总磷浓度显著减小与湖滨带植被的拦截作用密切相关;③ 2005—2018年滇池湖滨带草本和木本植物面积都显著增加,增加最剧烈的年份是2010—2011年;④ 相比于平水期和丰水期,枯水期NDVI与强影响区总磷浓度负相关关系最强,且木本植物NDVI与强影响区总磷浓度负相关性强于草本。本文利用长时间序列数据评价滇池总磷浓度与其湖滨植被的响应关系,可为滇池湖泊生态恢复提供科学依据。     

富营养化的太湖水体叶绿素a浓度模型反演

半经验模型反演叶绿素a浓度是目前遥感反演水体叶绿素a浓度的主要方法。但是,大量研究结果表明,太湖水体浑浊,富营养化严重,各种半经验模型的反演精度和模型适用性有较大差异。因此,研究一种既满足一定精度要求,又具有时间普适性的叶绿素a浓度反演算法,对提高模型适用性,促进遥感的反演应用具有重要意义。本研究通过2005年6-10月地面实测数据,建立太湖叶绿素a浓度一阶微分反演模型、波段比值反演模型和三波段反演模型,对比各模型反演效果,认为波段比值模型与三波段模型具有较好的反演效果。运用2006年11月和2007年11月实测数据对这三种模型加以检验,结果表明,三波段模型反演高富营养化的太湖水体,不仅精度高,平均误差仅为实测浓度差的8.3%,而且适用性较强,不同年份数据的检验结果证明平均误差均低于实测浓度差的20%。因此,三波段模型是这三种反演模型中效果最好的一类模型。     

Rotifer community structure and assessment of water quality in Yangcheng Lake

Rotifer community structure was investigated in Yangcheng Lake in 2008. Dominant species and species diversity indices were determined and QB/T was used to assess water quality. The annual average density and biomass of the rotifers in Yangcheng Lake was 2 894 ± 1 006 ind./L and 12.47 ± 10.28 mg/L, respectively. The highest densities were observed in the western portion of the lake in March, but the highest biomass occurred in inflowing creeks in September. Within a year of monitoring in Yangcheng Lake, 93 species were identified and the dominant species were found to be Polyarthra trigla, Brachionus angularis, Keratella cochlearis, Keratella valga, Brachionus calyciflorus, and Filinia major. Of the species recorded, 75 were pollution indicator species. Density and biomass exhibited significant positive correlations with water temperature (R = 0.209, P = 0.003; R = 0.446, P = 0.000), but the peak density showed two lags in response to chl a. According to the Jaccard similarity index (SJ), the greatest similarity among dominant species occurred between creeks and the eastern part of the lake. The annual average Shannon-Wiener diversity index H’, Margalef richness index D and Pielou evenness index J were 1.96 ± 0.34, 1.61 ± 0.50 and 0.77 ± 0.10, respectively. In all four areas of Yangcheng Lake, β- mesosaprobic species comprised the largest share of pollution indicator species. These data suggest that Yangcheng Lake is mesosaprobic.     

A study on the age and growth of Lake Anchovy (Coilia ectenes taihuensis Yuen et Tin) in Taihu Lake

Scales of the Lake Anchovy in Taihu Lake are used as a basis for its age determination. Annual variation of scale edge growth rate (α) indicates that the annulus forms once a year, mainly in April–June. The relationship formula for body length and scale diameter is L=18.658+118.06R, that for body length and weight isW♀=7.588×10⁻⁶ L ^2.8301;W♂=5.584×10⁻⁶ L ^2.8781. The fish before age II have a higher relative growth rate in body length and weight and a higher growth index. Growth is consistent with the growth equation by Von Bertalanffy. The turning point of the body weight growth curve is located between age II and III; the exact position is atW=0.292W _∞. Variation specificity of the growth course can be reflected by the growth rate and its acceleration curves. Time for capture of the fish should preferably be after age II, when they have reached a body length of 170 mm. That is one of the key measures to increase Lake Anchovy production in Taihu Lake.     

Internal Loads and Bioavailability of Phosphorus and Nitrogen in Dianchi Lake,China

Sediments have a significant influence on the cycling of nutrient elements in lake environments. In order to assess the distribution characteristics and estimate the bioavailability of phosphorus and nitrogen in Dianchi Lake, organic and inorganic phosphorus and nitrogen forms were analysed. The 210 Pb radiometric dating method was employed to study temporal changes in the phosphorus and nitrogen pools in Dianchi Lake. The result show that the total phosphorus(TP) and total nitrogen(TN) were both at high concentrations, ranging from 697.5–3210.0 mg/kg and 1263.7–7155.2 mg/kg, respectively. Inorganic phosphorus(IP) and total organic nitrogen(TON) were the main constituents, at percentages of 59%–78% and 74%–95%, respectively, in the sediments. Spatially, there was a decreasing trend in phosphorus and nitrogen contents from the south and north to the lake centre, which is related to the distribution pattern of local economic production. The burial rates of the various phosphorus and nitrogen forms increased in same spatially and over time. Particularly in the past two decades, the burial rates doubled, with that TN reached to 1.287 mg/(cm~2·yr) in 2014. As the most reactive forms, nitrate nitrogen(NO_3-N) and ammonia nitrogen(NH_4-N) were buried more rapidly in the south region, implying that the potential for releasing sedimentary nitrogen increased from north to south. Based on their concentrations and burial rates, the internal loads of phosphorus and nitrogen were analysed for the last century. A TP pool of 71597.6 t and a TN pool of 81191.7 t were estimated for Dianchi Lake. Bioavailable phosphorus and nitrogen pools were also estimated at 44468.0 t and 5429.7 t, respectively, for the last century.     

济南市华山湖开挖建设渗漏分析研究

该文在查明区域水文地质条件、湖区地下水的类型、分布、埋藏条件、含水层及隔水层特征、岩性结构以及地下水的补给、径流、排泄条件的基础上,通过现场试验,确定了天然状态下湖区各含水层之间的水力联系、黄河侧渗补给量及湖区向小清河的排泄量,结合数值模拟,预测了湖区维持设计蓄水位21.5m时黄河的侧渗补给量、湖区向小清河的排泄量及湖区对周边地下水位的影响。结果表明,天然状态下,黄河侧渗补给量为748.23m~3/d,湖区向小清河排泄量大约为52.03m~3/d,黄河侧渗补给量远大于湖区向小清河的排泄量。数值模拟结果表明,湖区维持设计蓄水位21.5m时,不同水文年湖区接受的侧渗补给量均大于湖区的渗漏量,且对周围地下水位的影响不超过1.5m,因此,湖区维持设计蓄水位21.5m时可不考虑防渗措施。