4种海蕴多糖的提取分离及理化性质

以海蕴(Nemacystus decipiens)为研究对象,经85%乙醇脱脂后,通过热水和2%碳酸钠提取并结合乙醇分级法获得了4个多糖组分(NW1~2和NA1~2)。运用HPIC、HPGPC及红外光谱(IR)等方法对其单糖组成、相对分子量以及基本结构特征进行了比较。结果表明,NA1是褐藻胶,经1H-NMR分析,其M/G为0.34。NA2及NW1~2均属于岩藻聚糖硫酸酯,且岩藻糖含量均达80%以上。NW1和NW2中岩藻糖(Fuc)、葡萄糖(Glc)、半乳糖(Gal)、木糖(Xyl)和鼠李糖(Rha)摩尔比分别为100∶5∶3∶16∶1和100∶5∶4∶4∶1。NA2中不含葡萄糖,其Fuc、Gal、Xyl和Rha摩尔比为100∶13∶1∶2。多糖NW1~2和NA1~2的相对分子量分别为939.8,1001,223.6和402.3 kD。     

三种绿藻多糖的提取及理化性质和活性比较

采用80℃水浴8h、乙醇沉淀、Sevage法去除蛋白质的方法提取湛江裂片石莼、肠浒苔和总状蕨藻多糖，并用Molish反应、Fehling反应鉴定多糖的化学性质，用苯酚-硫酸法测定多糖的总糖含量，用Dodgson法测定多糖的硫酸基含量，用Folin-酚法测定多糖的蛋白质含量．结果表明：3种绿藻的多糖提取率和多糖总糖含量以裂片石莼最高，分别为3．0％和72．6％，总状蕨藻最低分别为1．65％和38．6％；3种绿藻的硫酸基含量相差不大．总状蕨藻的蛋白质含量最高，为14．5％．3种多糖溶液作用于体外白血病细胞结果以总状蕨藻的抑制效果最明显，浓度为12mg／dm^3的总状蕨藻多糖溶液作用72h的抑瘤率达66％．     

海浪日最大波高序列的一种标度性质

应用重标度分析方法研究小麦岛海洋观测站１９６１－１９８０年二十年海浪日最大波高序列时发现，该序列并不是相互独立的，而是具有记忆性和持久性，详细的论证表明分式布朗运动模型是描述海浪日最大波高序列的一个较好的模型，同时，应用分式布朗运动模型我们找到了存在于海浪日最大波高序列中的一种标度性质－累积离差（ｔ）的统计自仿射性。     

4种不同来源浒苔中多糖的提取分离及理化性质

为探讨不同季节以及不同地点采集的浒苔其多糖理化性质差异,采用水提醇沉法对黄海海域青岛沿海春(原料1)秋(原料3)两季采集的浒苔、2008年夏季爆发浒苔(原料2)以及东海海域福建沿海采集的浒苔(原料4)的多糖进行了提取,得到了4种冷水提取多糖PC1-4和4种热水提取多糖PH1-4。分别对8种浒苔多糖利用Q-Sepharose Fast Flow离子交换色谱法纯化后得到了12个组分。对各组分的总糖含量、糖醛酸含量、硫酸根含量以及蛋白含量进行了测定,利用气相色谱法测定了单糖组成,结果显示不同季节不同海域采集样品的化学组成及单糖组成不同。     

刺松藻水溶性多糖的提取分离及理化性质研究

本文以刺松藻(Codium fragile)为原料,依次经冷水、热水提取,得到2种粗多糖CFC和CFH,经Q-SepharoseFF强阴离子交换色谱分离,分别从CFC和CFH中得到组分CFCP1～P6和CFHP1～P5,并对其理化性质进行了分析。分别运用高效凝胶渗透色谱法(HPGPC)、高效离子色谱法(HPIC)、傅里叶变换红外光谱法(FTIR)对其分子量、单糖组成和结构特征进行了分析。结果表明,刺松藻中多种结构复杂的多糖,CFCP1和CFCP6属于硫酸阿拉伯半乳聚糖,CFCP4和CFHP5属于丙酮酸化的硫酸半乳聚糖,CFHP1和CFHP2分别属于葡聚糖和甘露聚糖。多糖CFCP2、CFHP2和CF-HP3中除了含有半乳糖、阿拉伯糖、葡萄糖和甘露糖外,还含有木糖、鼠李糖和岩藻糖。这些结构特殊的多糖为海洋药物研究与开发提供了良好基础。     

一种来自海洋细菌的血纤维蛋白溶酶的分离纯化及性质研究

利用硫酸铵分级盐析、DEAE－纤维素阴离子交换层析、Sephadex G-75凝胶层析法对由海洋细菌FE92－8分泌的纤维蛋白溶酶进行了分离纯化，结果得到SDS－聚丙烯酰胺凝胶电泳单一区带。性质研究发现，该酶分子量为12.6kD，等电点为7.45，琼脂糖－纤维蛋白平板法测得该酶作用的最适温度为50℃，最适pH为8.0，在37℃，pH为8.0的情况下表现出很好的稳定性，温度起来50℃热稳定性较差，在碱性环境中稳定性较在酸性环境中稳定性强，阴性平板和阳性平板对比实验发现，该酶只有纤溶活性而无激酶活性，体外实验发现，该酶具有快速溶解血栓的能力。     

几种海藻粗多糖的理化性质及结构特征分析

主要采用傅里叶红外光谱(fourier transform infrared spectroscopy, FTIR)、气相色谱(gas chromatography, GC)和凝胶渗透色谱(gel permeation chromatography, GPC)方法对5种海藻粗多糖的单糖组成、官能团、分子量进行了初步分析。结果显示5种海藻粗多糖均含有木糖和岩藻糖, 其中南方团扇藻多糖含有较为丰富的山梨糖, 属于大分子量均一性多糖, 而孔石莼多糖分子量分布较宽, 均一性较差。红外光谱分析结果表明, 5种粗多糖均为酸性多糖, 棒叶蕨藻、琼枝麒麟菜和匍枝马尾藻多糖具有明显的硫酸基团吸收峰, 琼枝麒麟菜多糖存在呋喃糖和吡喃糖, 并且吡喃糖苷键可能存在β和α两种类型。明胶氯化钡实验结果指出南方团扇藻多糖的硫酸基团含量为38.43%(±2.38%), 明显高于其他几种海藻多糖, 而棒叶蕨藻多糖的硫酸基团含量最低, 仅为8.92%(±1.04%)。     

海绵内生真菌胞外多糖理化性质及自由基清除活性研究

从1株海绵内生真菌(Alternaria sp.)链格孢菌的发酵液中提取胞外多糖,对其进行Q Sepharose FF离子交换色谱分离纯化,得到2个组分JJY-W和JJY-S。运用各种化学方法及波谱方法对JJY-W和JJY-S的理化性质及结构进行分析,并对多糖清除自由基活性进行了评价。结果表明,JJY-W和JJY-S的分子量分别为1.4KD和1.8KD;JJY-W以半乳糖、葡萄糖为主,含有少量甘露糖,摩尔比为:甘露糖∶葡萄糖∶半乳糖=1∶2.5∶11。JJY-S以甘露糖、葡萄糖为主,含有少量半乳糖,摩尔比为:甘露糖∶葡萄糖∶半乳糖=3∶2∶1。JJY-W总糖含量为46.3%,未检测出糖醛酸,蛋白含量为2%。JJY-S总糖含量为52%,糖醛酸含量为6.1%,蛋白含量为14%。活性分析表明,2种多糖均具有一定的体外抗氧化活性,并随着浓度的增加清除自由基能力均增强,JJY-W清除DPPH自由基的活性要强于JJY-S,而JJY-S清除OH.自由基的活性要强于JJY-W。     

几种红藻琼脂的组分结构及理化性质的比较

以我国3种经济红藻龙须菜、石花菜、坛紫菜为原料，对高温稀碱法、常温浓碱法和无碱法提取的琼脂进行理化性质和化学组分结构方面的横向比较。结果表明，所提取琼脂的主要组分均为无取代的琼脂糖；凝固温度与甲氧基的取代位置及含量有关；我国3种琼脂海藻均为优质的琼脂原料；提取琼脂时，石花菜不用碱处理，龙须菜和坛紫菜则必须经过碱处理；从灰分和硫酸基含量角度分析，石花菜琼脂优于坛紫菜琼脂后，后者又优于龙须菜琼脂。     

副溶血弧菌一种糖蛋白酶(复苏促进因子)在大肠杆菌中的表达及性质

用PCR方法从副溶血弧菌8621.4基因组中扩增出1种细胞复苏促进因子家族的糖蛋白酶(Glycoprotease,Gcp)基因,核酸序列分析表明其含有完整的gcp基因开放阅读框,编码233个氨基酸组成的蛋白质.核酸序列与副溶血弧菌糖蛋白酶家族基因的序列相似性为100％.其氨基酸序列与哈维氏弧菌HY01、弧菌Ex25、溶珊瑚弧菌、拟态弧菌和杀鲑弧菌LFI1238等糖蛋白酶的序列相似性为67％～92％.将该基因克隆到表达载体pET28a,在大肠杆菌BL21 (DE3)中诱导表达,用Ni琼脂糖亲和柱层析纯化的蛋白为单一条带,利用纯化的Gcp免疫新西兰大白兔制备特异性抗体,Western-Blotting分析发现正常生长及活的非可培养状态(VBNC)诱导过程中的副溶血弧菌细胞内表达的Gcp蛋白为1条带,分子量约为27 kDa,而在VBNC状态的菌体中检测到2条蛋白带.研究结果为进一步探索海洋弧菌活VBNC的形成和复苏机制奠定基础.     

Mechanical and Germination Characteristics of Stabilized Organic Soils

High-organic-content dredged soils are known to have inferior mechanical characteristics because they are highly compressible and have low shear strength. To recycle dredged soil with a high organic content as a top soil this study describes an investigation of the mechanical properties and germination characteristics of stabilized organic soils using unconfined compression tests, pH tests, and seed germination tests. Several mixtures with organic contents in the range 0–30% by mass and binder contents in the range 5–15% were prepared to evaluate the effects of the organic content on the mechanical and germination characteristics of the stabilized soils. The results show that an increase in the organic content leads to a decrease in the strength and pH of the stabilized organic soil, which are favorable conditions for germination. The germination rate increased significantly with the increasing organic content, and the plant growth rate also increased. The addition of a binder into the mixtures increased the strength of the soil; however, it also increased the pH and decreased the rate of seed germination and plant growth.     

南沙群岛珊瑚砂物理力学性质研究

珊瑚砂是发育于热带海洋环境中特殊的岩土介质类型,主要由珊瑚碎屑和其它海洋生物碎屑组成,碳酸钙含量高达96％。特殊的发育环境、物质组成和结构导致了珊瑚砂具有独特的物理力学性质。本文对采白南沙群岛珊瑚岛礁的珊瑚砂样品进行了。系列室内土力学试验,根据试验结果讨论了珊瑚砂的比重、孔隙比、压缩性、破碎性、剪切特性等,指出了珊瑚砂具有不同于石英砂的比重和孔隙比等物理特性,力学性质的特殊性表现在三个方面：内摩擦角大,高压缩性和易破碎。作者认为颗粒破碎是影响珊瑚砂变形和强度特性的主要因素。全面深入地研究珊瑚砂的基本物理力学性质,可为珊瑚岛礁工程建设提供科学合理的设计参数,避免工程事故的发生。     

On the Problems of Epibioses, Fouling and Artificial Reefs, a Review

Abstract. Artificial reefs in marine environments are in most cases submerged structures consisting of dumped waste material or specific constructions made with the purpose of enriching the local fish populations and other marine life to the benefit of recreational and commercial fisheries. Such structures are susceptible to fouling and will successively develop assemblages, which may or may not resemble epibioses on natural substrata. Studies of artificial reefs have focused predominantly on fish assemblages and have largely disregarded the development of sessile biota and their structural and functional relationships. In addition, most studies are from tropical or subtropical environments. To manage and understand artificial reefs, a whole-ecosystem approach is necessary, incorporating studies of all aspects of hard substratum ecology including both structural and functional variables. This review is an attempt to evaluate the present knowledge of ecological aspects of artificial reefs, emphasising the role of sessile hard substratum biota.     

地下水库人工补给的模型研究--以大沽河地下水库为例

为使地下水库在丰水季节获得足够补给,利用Visual Modflow 软件,在分析地下水库水文地质条件的基础上,概化出该区水文地质概念模型,运用准三维地下水水流模型,通过有限差分方法对地下水库的地下水系统进行了模拟,拟合结果表明, 地下水位的计算值与实测值拟合程度较好,模拟出的地下水流场与实际情况基本一致.利用校正好的模型,分析了橡胶坝渗漏补给的动态变化规律,并针对橡胶坝的补给,通过数值模拟探讨了坝内蓄水因工业开采而激发的渗漏补给量.结果显示,橡胶坝的渗漏补给量在枯水期（2～5月份）逐渐增大,而在平水期（9～12月份）逐渐减小;激发补给量与激发开采量的比值呈现上升趋势,同时随着激增补给量的迅速增大,激发补给量与开采增加量的比值随工业开采量的增加而逐渐增大,这说明傍橡胶坝取水可使更多的地表水入渗补充地下水,从而有效地提高水资源的利用率.     

小型单拖网渔船V 型网板水动力性能研究

网板是拖网的重要属具之一, 广泛应用于近岸小型单拖渔船的生产作业中, 其水动力性能的优劣直接关系到拖网的生产效果和经济效益。作者以网板面积1 m²、展弦比0.55的 V 型网板为例进行小型单拖渔船的水动力性能研究。 基于田内相似准则, 相似比为2制作网板模型, 进行循环水槽模型试验, 实验冲角在10～60°范围内, 来流速度在0.2～0.7 m/s, 得出网板升阻力系数等流体动力特性曲线、临界冲角、最大升阻比。结果表明, 该型网板的最大升阻比约为1.86, 达到最大升阻比的临界冲角在15～25°附近, 该结果可为渔业生产操作中网板冲角的设置提供依据。实验结果同时显示, 该型网板的水动力效率略低于其他类型网板, 说明其板型仍有较大的改进空间。     

Generation of Small Meanders of the Kuroshio South of Kyushu in a High-Resolution Ocean General Circulation Model

The generation of small meanders of the Kuroshio south of Kyushu has been investigated using a high-resolution ocean general circulation model of the North Pacific Ocean. The small cyclonic meander develops in the region east of the Tokara Strait with a period of about one month, then propagates downstream along the Kuroshio path to the longitude of the Kii Peninsula, which is similar to the so-called trigger meanders for the formation of the large-meander of the Kuroshio south of Japan. It turns out that the generation of the small meander is a local phenomenon, strongly associated with anticyclonic eddies that propagate northeastward along the Kuroshio path in the East China Sea. The vorticity balance indicates that the accumulation of positive vorticity during the developing phase of the small meander occurs mainly from the balance between the stretching and the advection terms. This revised version was published online in July 2006 with corrections to the Cover Date.     

Numerical Study of a Kyucho and a Bottom Intrusion in the Bungo Channel,Japan: Disturbances Generated by the Kuroshio Small Meanders

The generation and propagation mechanisms of a Kyucho and a bottom intrusion in the Bungo Channel, Japan, have been studied numerically using the hydrostatic primitive equations by assuming density stratification during summer. The experiments are designed to generate a Kuroshio small meander in Hyuga-Nada, which acts as a trigger for these disturbances. After the current speed of the Kuroshio is changed, a small meander is generated. At the head of the small meander, warm Kuroshio water is engulfed, and encounters the southwest coast of Shikoku. However, convergence of heat flux on the bump off Cape Ashizuri suppresses the generation of a warm disturbance, if the current speed is large. As the cold eddy associated with the small meander approaches Cape Ashizuri, the heat flux diverges on the bump. This heat source forces a warm disturbance, which intrudes along the east coast of the Bungo Channel as a baroclinic Kelvin wave (a Kyucho). After the cold eddy passes off Cape Ashizuri, the Kuroshio approaches the bump again. Strong convergence of heat flux then occurs on the bump, which forces a cold disturbance. This disturbance propagates as a topographic Rossby wave along the shelf break at the mouth of the channel. After the topographic wave reaches the west end of the shelf break, it intrudes along the bottom layer of the channel as a density current (a bottom intrusion). These results suggest that a Kyucho and a bottom intrusion are successive events associated with the propagation of the small meander.     

Changes in the Structure of a Coastal Fish Assemblage Exploited by a Small Scale Gillnet Fishery During an El Niño–La Niña Event

During 1998 an experimental gillnet fishing survey was carried out in a Mexican Central Pacific inshore zone. One-hundred and thirty fish species belonging to 51 families and 18 orders were identified. The most abundant species wereMicrolepidotus brevipinnis (29·0% of the total abundance) and Caranx caninus (19·2%), followed by C. caballus (6·3%), Kyphosus analogus (4·3%) and C. sexfasciatus (3·4%). Thermal SST anomalies showed the existence of two periods. The first, from January to April with positive anomalies, defines the end of an El Niño episode. The second period, from May to December, constitutes the beginning of the La Niña episode. The typical seasonality in a non-anomalous year continued for a large percentage of the inshore fish community, and the effects of the anomalous event consisted of changes in seasonality of occurrence in some individual species and the unusual abundance of some uncommon species. The species richness was higher during the El Niño–La Niña event than in a non-anomalous year, and therefore the event could be considered an interannual environmental mechanism that favours fish diversity in inshore waters.     

基于生态化人工海岸建设的理论研究与应用 ——以天津永定新河口综合整治修复工程为例

分析生态文明背景下围填海区域人工海岸建设存在的问题,结合淤泥质人工海岸空间特点,总结提出了生态岸线整治修复技术、仿自然化处理设计、景观构建适宜性、人工海岸生态系统等生态化建设基础理论,并以天津永定新河口综合整治修复工程为例,重点阐述了人工海岸生态化建设过程中生态系统和景观构建的方法要点和具体思路.结果表明:在人工海岸生...