A method for remote detection of oil spills using laser-excited Raman backscattering and backscattered fluorescence

A laser method for the remote detection of oil present as a pollutant in the sea water using Raman backscattering and backscattered fluorescence is discussed. The scattering spectra of oils obtained by using a laser Raman spectrometer that employs a CW Ar laser are described. The backscattering spectra of oils obtained by a laser radar technique in the laboratory and the field are also described. Furthermore, theoretical performance of a laser radar with a CW laser or a pulsed laser for the detection of Raman backscattering of kerosene is discussed.     

基于分光光度法的多量程海水营养盐原位传感器检测系统设计

针对传统海水营养盐检测方法不能满足海水营养盐长期原位监测需求的问题,研制了一种基于分光光度法的多量程海水营养盐原位传感器检测系统,通过对系统的高度集成及对多量程检测、低功耗技术、漏液保护技术的应用,实现了对海水5项营养盐参数快速、宽范围、高精度的原位测量。经过实验室和青岛中苑码头现场测试,表明本营养盐传感器检测系统具有低功耗、高可靠性能,可满足对5项营养盐参数的快速精确测量要求,实现了对海水营养盐参数的原位监测,为相关部门及时了解海洋生态环境和水体富营养化程度提供了数据支持,具有重大现实意义。     

Measurement of water column primary production using photosynthesis-irradiance relations for surface phytoplankton,the vertical chlorophyll profile,and underwater light intensity

A method has been developed to measure water column integrated primary production (PP_int) in the water column using photosynthesis-irradiance relations for surface phytoplankton, the vertical profile of chlorophyll a concentration, and the underwater light intensity. Good correlation has been found for the results calculated with this method and light dependences in situ. The advantages of this method are the independence of PP_int calculation from CTD profiling and water sampling, and thus optimization (reduction) of the station working time.     

A sensor package for the simultaneous determination of nanomolar concentrations of nitrite, nitrate, and ammonia in seawater by fluorescence detection

A fluorescence-based chemistry has been developed for the detection of nitrite and nitrate (as excess nitrite following reduction of nitrate to nitrite). Detection limits are 4.6 and 6.9 nM, respectively. The technique capitalizes on the triple bond between the two nitrogen atoms within the diazonium ion formed via the well-known reaction between an acidified nitrite sample and an aromatic primary amine. Fluorescence of π-electrons within this bond allows this reaction to be probed with standard fluorescence spectroscopy. Reverse Flow Injection Analysis (rFIA) is used to correct for background fluorescence from leachates and naturally occurring dissolved organic matter (DOM). Comparisons of samples analyzed for nitrite with this technique and with a highly-sensitive chemiluminescent method [Braman, R.S., Hendrix, S.A., 1989. Nanogram nitrite and nitrate determination in environmental and biological materials by vanadium (III) reduction with chemiluminescence detection. Analytical Chemistry, 61 (24) 2716–2718] showed excellent agreement between the two methods (slope=0.9996 and r²=0.9956). These fluorescent nitrite and nitrate + nitrite chemistries were coupled in a sensor package with a modified version of a fluorescent ammonia chemistry [Jones, R.D., 1991. An improved fluorescence method for the determination of nanomolar concentrations of ammonia in natural waters, Limnology and Oceanography. 36(4) 814–819], which also has a nanomolar detection limit. The throughput rate of the fully automated three-channel instrumentation is 18 samples per hour. A field experiment demonstrated the capability of the nutrient sensor package to determine horizontal gradients in nitrate, nitrite, and ammonia in oligotrophic surface waters.     

Modeling of the annual and spectral evolution of hydrooptical characteristics using a model for shelf ecosystem and remote observations

Mathematical modeling of the ecosystem in the northwestern shelf of the Caspian Sea is based on the method of aggregation and averaging with the subsequent hierarchic decomposition. This approach includes a step-by-step comparison of the modeling results with the data of observations. A block of optical characteristics (absorption coefficient, backscattering coefficient, and coefficient of diffuse light reflection) built into the model made it possible to use the data of simultaneous contact and remote observations. The observations were performed during the expedition of R/V Tantal (2004–2005) together with the data of the SeaWiFS satellite ocean color scanner. A comparison of the results of the modeling and the data of observations demonstrated their qualitative and quantitative agreement, especially in the case of the coefficient of diffuse light reflection.     

Species-specific detection and quantification of scyphomedusae in Jiaozhou Bay,China, using a quantitative real-time PCR assay

Over the past decades, jellyfish occurred increasingly and abundantly in coastal areas worldwide. Usually, biomass of jellyfish, especially when they bloom, can...     

激光单分子BTEX异常的人工神经网络评价技术

采用BP(Back-Propagation Network)神经网络方法,根据试验区BTEX指标实测数据,结合油气化探、地质、地球物理等资料建立BTEX异常综合评价指标体系及评分标准,完成BTEX异常的BP神经网络综合评价模型并对试验区进行含油气远景评价,研究结果表明该技术具有较好的应用前景。     

Mapping the change of coral reefs using remote sensing and in situ measurements: a case study in Pangkajene and Kepulauan Regency,Spermonde Archipelago,Indonesia

As elsewhere in Indonesia, local inhabitants in the Pangkajene and Kepulauan (PANGKEP) Regency, Spermonde Archipelago area and along the south-west coast of Sulawesi traditionally regard the coral reefs as their livelihood source. Since human activities as well as natural disturbances pose major threats to the coral reefs, these livelihoods may also be at risk. Currently, no comprehensive information on the status and condition of coral reefs in this area is available for this resource management. We determined the changes of coral reef habitat over a period of 20 years from 1994 to 2014 using a satellite Landsat multi-temporal image substantiated with in situ measurement data collected in 2014. The spectral value of coral reefs was extracted from multi-temporal Landsat imagery data, while the diffuse attenuation coefficient of water was obtained by using statistical analysis between the ratio of live coral cover and the spectral value of the visible bands. By using the unsupervised classification integrated with the data ground truth, it is stated that there has been a decline in live coral cover over a period of 20 years from 7716 ha in 1994 to 4236 ha in 2014, with a degradation rate of 174 ha/year. Based on the results, the ratio of the coral cover in the coral reef transects varied from the average of 24% for live corals to 96% for coral rubbles, implying the degraded status of coral reefs in the study area.     

Seasonal physical–chemical structure and acoustic Doppler current profiler flow patterns over multiple years in a shallow, stratified estuary, with implications for lateral variability

The overall goal of this study was to strengthen understanding of the hydrographic structure in shallow estuaries as influenced by seasonal and depth-dependent variability, and by variability from extreme meteorological events. The mesohaline Neuse Estuary, North Carolina, U.S.A., which was the focus, receives surface inputs from upriver and tributary freshwater sources and bottom inputs from downriver high-salinity sound water sources, resulting in varying degrees of stratification. To assess depth-dependent, estuary-wide changes in salinity, a multiple time series was created using data from four discrete depths (surface and 1, 2, and 3 m±0.25 m). The database was developed from weekly to biweekly sampling of the entire water column, and included side-channel as well as mid-channel data. We characterized seasonal differences in halocline depth affecting the hydrographic structure of the mesohaline estuary and site-specific variation in nutrient concentrations, based on a comprehensive eight-year physical/chemical database. The first two years of the record showed an expected seasonal signal and included events that impacted the surface layer from freshwater inputs. Remaining years had greater variability over seasons and depths, with freshening events that affected all depths. Halocline depth was compared at specific locations, and a “snapshot” view was provided of the relative depth of these water masses within the estuary by season. We also examined flow patterns at the same cross-estuary sites over a three-year period, using a boat-mounted acoustic Doppler current profiler (ADCP) with bottom-tracking capability. Composite visualizations constructed with single-transect ADCP data revealed a classical estuarine circulation pattern of outflow at the surface/southern shore and inflow at the bottom/northern shore. Although this pattern deviated under extreme climatological events and was sometimes variable, the estuary generally exhibited a high probability of direction of flow. Wind fields, hurricanes, and small-scale, high-precipitation events represented significant forcing variables.     

Finding a balance between economic performance and capacity efficiency for sustainable fisheries: Case of the Da Nang gillnet fishery,Vietnam

Marine capture fisheries in Vietnam are small-scale, multi-species, multi-gear, and open access regime. Under numerous governmental promotion programs, Vietnamese fishing fleets have expanded at an uncontrollable rate. This research analyzes the economic performance and capacity efficiency of the Da Nang gillnet fishery. Data were obtained from 45 gillnetters in Da Nang using questionnaires, which were administered between April and July of 2011. The results showed that large-scale vessels (engine power≥90 hp) had better economic performance than small-scale vessels (engine power<90 hp) with average gross revenue flows of 1346.7 and 750.7 million VND, respectively. The large-scale vessels further actualized positive profits (15% gross revenue), while the small-scale vessels demonstrated negative results (−0.4% gross revenue). The main operating costs of fuel and labor accounted for 44% and 26%, respectively, of the total operating expenses. However, the results of the data envelopment analysis (DEA) showed that the Da Nang gillnet fishery did not operate at full capacity and that the unused capacity and the technical inefficiency rates were approximately 21.2% and 10.8%, respectively. The over-use of fuel, net sheets, and number of days at sea in the large-scale vessels were 7%, 10%, and 3%, respectively, while these ratios in the small-scale vessels were 7%, 3%, and 7%, respectively. Overcapacity may be separated into two parts, one part resulting from the optimal technical use of inputs and a second part resulting from enhancing the current policies. As such, feasible policies may include implementing the buyback of small and unprofitable vessels, and reviewing subsidy programs.     

Evaluation of the performance of HPLC–CHEMTAX analysis for determining phytoplankton biomass and composition in a turbid estuary (Schelde, Belgium)

In the upper Schelde estuary in 2002, phytoplankton biomass and community composition were studied using microscopic and pigment analyses. Chlorophyll a concentration was a good predictor of phytoplankton biomass estimated from cell counts and biovolume measurements. The phytoplankton carbon to chlorophyll a ratio, however, was often unrealistically low (<10). CHEMTAX was used to estimate the contribution of the major algal groups to total chlorophyll a. The dominant algal groups were diatoms and chlorophytes. While diatom equivalents in chlorophyll a predicted diatom biomass relatively well, chlorophyte equivalents in chlorophyll a were only weakly related to chlorophyte biomass. The pigment-based approach to study phytoplankton overestimated phytoplankton biomass in general and chlorophyte biomass in particular in late autumn and winter, when phytoplankton biomass was low. A possible explanation for this overestimation may be the presence of large amounts of vascular plant detritus in the upper Schelde estuary. Residual chlorophyll a, chlorophyll b and lutein in this detritus may result in an overestimation of total phytoplankton and chlorophyte biomass when the contribution of phytoplankton to total particulate organic matter is low.