Survival of two species of amphipods in aqueous extracts of petroleum oils

Aqueous extracts of two petroleum oils, No. 2 fuel oil and Southern Louisiana crude, were tested on two amphipods, Gammarus muccronatus and Amphithoe valida, for survival. The oils were toxic at concentrations of 0.8 ppm (fuel oil) and 2.4 ppm (S. Louisiana crude). Mortalities increased with the concentration and length of exposure. Few or no young were produced at these and higher concentrations (breeding adults were decreasing rapidly in numbers). The amphipods are more sensitive to aqueous extracts of these oils than benthic polychaetes and shrimp, for which data are available.     

Effects of Scotian shelf natural gas condensate on the mummichog

The 96h LC₅₀ of the water soluble fraction (WSF) of natural gas condensate from the Sable Is., N.S. area to Fundulus heteroclitus was between 4 and 5 μg · ml⁻¹ naphthalene equivalents (initial concentration). Behavioural responses in the fish to sub-lethal concentrations of WSF were similar to those recorded for crude and fuel oils. Induction of the hepatic mixed function oxidase enzyme enthoxyresorufin O-de-ethylase (EROD) in sexually immature fish occurred at WSF concentrations below the LC₅₀, but during the prespawning period no induction occurred. EROD activity in unexposed fish showed seasonal variation consistent with differences in its inducibility.     

Application of entropy analysis of in situ droplet-size spectra in evaluation of oil chemical dispersion efficacy

In situ droplet-size distributions were measured using a laser in situ scattering and transmissiometry (LISST-100X) particle size analyzer during the evaluation of natural and chemical dispersion efficiency of crude oils under different wave and current conditions. An entropy grouping of the in situ dispersed oil droplet-size spectra has classified the multi-modal droplet-size distributions into different groups based on similar droplet-size spectra characteristics within groups and distinction between groups. A generalized linear logistic regression model was fitted to analyze the effects of a number of factors and their interactions on the grouping of oil droplet-size spectra. The grouped results corresponded to the oil dispersion efficiency at different levels. This new method for droplet-size distribution data analysis can have significant implication in field evaluation of natural and chemical dispersion efficiency of oil.     

Effects of surface and sunken crude oil on the behaviour of a sea urchin

Studies have been made of the effects of exposure to various forms of crude oil on the righting behaviour of Paracentrotus lividus and its reactions towards the presence of oil. Prolongation of the righting response was recorded in animals exposed to contact with surface or sunken fresh crude oil or to their water soluble fractions. No such effect was recorded on exposure to weathered oils and results indicate that the more volatile components of crude oil were responsible for this effect. Paracentrotus showed no avoidance reaction to the presence of sunken oil in its vicinity. The likely ecological significance of these results is discussed.     

Photo-induced toxicity of north sea crude oils toward bacterial activity

Toxicity of photolysis products from Ekofisk and Statfjord crude oils toward natural assemblages of marine bacteria was investigated. Incorporation of ¹⁴C-glucose, ¹⁴C-amino acids or ³H-thymidine were used as indicators of microbial activity. While none of these measurements revealed any significant effects of non-illuminated oil, a toxic effect was found after a few hours illumination of an oil slick.     

Oil pollution and seabirds: Atlantic Canada 1976–1977 and some implications for northern environments

Chemical analyses of the oils on dead or moribund seabirds from Atlantic Canada during the winter of 1976–1977 indicated that some of the birds were contaminated with oil spilled by the Argo Merchant grounding, some by oil probably from the Grand Zenith sinking, and still others by oil from various small local spills of unknown origin. These victims demonstrated that an extremely minute oiling may lead to the death of a seabird when the effects of the oil are synergistically combined with the stresses imposed by severe environmental conditions. This may intensify the impact of oil in the Arctic and thereby have a profound effect on seabird populations.     

Genetic features of gas-condensate reservoirs in the Kekeya Field,southwest depression in the Tarim Basin,China

Oils, condensates and natural gases in the Kekeya Field, southeast depression of the Tarim Basin were studied for their geochemical characteristics. According to the distribution analysis of the C₂/C₃ values with C₁/C₂ values, C₂/C₃ values with C₁/C₃ values, as well as C₂/C₃ values with dryness index, there are two different types of natural gases in the studied field, which are spatially regularly distributed. One is the oil cracking gas, located on shallow reservoirs over X ₅ ² reservoir, namely Upper oil legs; the other is kerogen cracking gas, located on X ₇ ² reservoirs, X₈ reservoirs and E₂ k reservoirs, namely Lower oil legs. In addition, the distribution patterns of molar concentration of oils and condensates with different carbon numbers of the n-alkanes in the Kekeya Field indicate that the crude oils have experienced several kinds of secondary alterations, which were closely related to the charging of gaseous hydrocarbons after petroleum accumulation. These results indicate that, based on the research of δ ¹³C values of individual hydrocarbons, heptane values and isoheptane values of light hydrocarbons and aromatic maturity parameters for oils, condensates and natural gases, oils and gases were charged at different geological time in the Kekeya Field.     

Effect of oils on cell membrane permeability in Fucus serratus and Laminaria digitata

Leakage of electrolytes from discs of treated fronds of Laminaria digitata and Fucus serratus was the criterion used to study the effect of oils over 24 h on cell membrane permeability. Motor fuel oil increased cell membrane permeability in both algae at 1 h, or longer, after treatment. Laminaria changed to a green colour after 3 h, but Fucus was unaffected visibly by the oil. White spirit, diesel oil, or aviation fuel did not alter cell membrane permeability or visibly affect either alga over 24 h.     

Recovery of interior brackish marshes seven years after the chalk point oil spill

Seven years after the April 2000 spill of 140,000 gallons of a mixture of No. 6 and No. 2 fuel oils in the Patuxent River, Maryland, heavily oiled brackish marshes showed continuing effects. Stem density and stem height were significantly lower in oiled versus unoiled sites for Spartina alterniflora but not Spartina cynosuroides habitats. In contrast, belowground biomass was significantly lower in S. cynosuroides habitats but not S. alterniflora habitats. Total PAH concentrations were up to 453 mg/kg in surficial soils (0-10 cm) and 2921 mg/kg with depth (10-20 cm). The oil had lost 22-76% of its initial PAH content after seven years, although the oil in marsh soils has undergone little to no additional weathering since Fall 2000. Based on amphipod acute toxicity tests and sediment quality guidelines, 25% of the soils in the marsh are expected to be toxic (ESB-TU_FCV values > 3.0; P_Max > 0.65).     

Oil spill clean-up: The effect of three dispersants on three subtropical/tropical seagrasses

Three seagrasses found throughout the Greater Caribbean tropical/subtropical region as major critical habitat organisms were tested in the laboratory for toxicity limits to three dispersants commonly stockpiled in the region. At concentrations in the recommended dosage level, that is, below 1 ml dispersant with 10 ml oil in 100 000 ml seawater, even for 100 h no large mortality occurred (15–18 barrels per acre as calculated by Exxon, 1985). At an order of magnitude higher, especially for longer time periods, the more sensitive seagrasses Syringodium filiforme and then Halodule wrightii succumbed. The dispersants had widely differing effects, with Corexit 9527 and Arcochem D609 having far less toxic effect than Conco K(K) at the same exposure time and concentration. There was comparatively little difference between effects of oils (Louisiana crude versus Murban). Types and brands of dispersants should be referred to specifically in oil spill contingency plans since such widely varying ecological toxicity occurs among various dispersants. Use of the word ‘dispersant’ as a policy tool should be used with caution, realizing that dispersants vary widely in toxicity effects. Further testing of seagrasses in other ocean basins and those dispersants to be used there is highly recommended.     

Composition and sources of pollutant hydrocarbons in the Severn Estuary

Hydrocarbon distributions in sediment from the Severn Estuary can be explained mainly in terms of a pollutant origin. The aliphatic hydrocarbon distribution is similar to that found in biodegraded crude oils. The distribution of polycyclic aromatic hydrocarbons, including the carcinogen benzo(a)pyrene, is similar to that derived from the pyrolysis of fossil fuels, especially petroleum.     

Clam farmers and Oystercatchers: Effects of the degradation of Lanice conchilega beds by shellfish farming on the spatial distribution of shorebirds

The Manila clam Ruditapes philippinarum cultivation is an original shellfish farming activity strongly mechanized. In the Chausey archipelago (France) this activity settles on the Lanice conchilega beds, habitat known to host a rich and diversified benthic macrofauna and which is an attractive feeding ground for birds. A first study highlighted that this activity had strong negative effects on the L. conchilega beds and their associated benthic macrofauna. Here we assess the impacts of such an activity on the Eurasian Oystercatcher Haematopus ostralegus for which Chausey is one of the most important national breeding sites and which is also a common species in winter, spring and autumn migrations. We found that Oystercatchers significantly selected the L. conchilega beds to feed and that their spatial distribution was significantly modified after the creation of new clam concessions. In a context of a growing disappearance of pristine coastal ecosystems for the benefit of anthropo-ecosystems, we discuss the problem of the degradation of such benthic habitats with a low resilience which may loose their high functional value.     

Statistical Analysis and Modeling of the Local Ionospheric Critical Frequency: A Mid-latitude Single-Station Model for Use in Forecasting

The hourly values of the F-layer critical frequency from the ionospheric sounder in Dourbes (50.1°N, 4.6°E) during the time interval from 1957 to 2010, comprising five solar cycles, were analyzed for the effects of the solar activity. The hourly time series were reduced to hourly monthly medians which in turn were used for fitting a single station foF₂ monthly median model. Two functional approaches have been investigated: a statistical approach and a spectral approach. The solar flux F_10.7 is used to model the dependence of foF₂ on the solar activity and is incorporated into both models by a polynomial expression. The statistical model employs polynomial functions to fit the F-layer critical frequency while the spectral model is based on spectral decomposition of the measured data and offers a better physical interpretation of the fitting parameters. The daytime and nighttime foF₂ values calculated by both approaches are compared during high and low solar activity. In general, the statistical model has a slightly lower uncertainty at the expense of the larger number of fitting parameters. However, the spectral approach is superior for modeling the periodic effects and performs better when comparing the results for high and low solar activity. Comparison with the International Reference Ionosphere (IRI 2012) shows that both local models are better at describing the local values of the F-layer critical frequency.     

The relationships between particulate and soluble alkaline phosphatase activities and the concentration of phosphorus dissolved in the seawater of Toulon Bay (NW Mediterranean)

The activities of particulate and soluble phosphatase were analyzed monthly for 1 year in the coastal ecosystems of the NW Mediterranean Sea. The mean contribution of the particulate activity increased from 56% at an MUF-P concentration of 30 μM to 77% at 0.04 μM. This particulate activity was negatively correlated with the DIP, DOP and TDP concentrations when the activities were related to the seawater volume, chlorophyll a or the protein concentration. The TDP correlations were highly significant (p: 0.001). The DOP correlations were significant (p: 0.04) and became highly significant (p: 0.009) at low DIP concentrations (<0.13 μM). The DIP correlations were significant (p: 0.04) only at low DOP concentrations (<0.18 μM). Thus, the effects of seawater DIP and DOP were found to be linked. The soluble activity exhibited distinct phosphatase fractions with high (0.5–29.5 μM) and low (0.02–2 μM) Km values, but none exhibited significant correlations with phosphorus compounds.     

In vitro effects of nonylphenol on functional responses of haemocytes of the colonial ascidian Botryllus schlosseri

The effects of nonylphenol (NP) on functional responses of haemocytes of the colonial ascidian Botryllus schlosseri were evaluated. Haemocytes were exposed to 0.1, 1 and 10 μM NP and the effects on haemocyte viability, adhesion, morphology, lysosomal membrane stability, phagocytic activity and early apoptosis were evaluated. Haemocyte viability and adhesion were not affected by NP. Phagocytic activity and the amoebocytic index decreased significantly at all the concentrations tested, while exposure of haemocytes to 1 and 10 μM NP caused a significant increase in the diffusion of Neutral Red into the cytosol. The percentage of cells positive to Annexin-V (indicative of early apoptosis) increased significantly at 1 and 10 μM NP. Results obtained suggested a relationship between NP and alterations in functional responses of haemocytes in B. schlosseri. Biomarkers measured resulted sensitive, rapid and reproducible, even if their responsiveness will be evaluated after in vivo exposure of animals to NP.     

Advances in separating effects of climate variability and human activity on stream discharge: An overview

Separating effects of climate change (ΔQ^c) and human activity (ΔQ^h) on stream discharge at the watershed scale is needed for developing adaptive measures to climate change. However, information is scarce in existing literature regarding whether such separating is feasible and whether reliable results can be produced. The objectives of this overview were to: (1) compare currently-used methods; (2) assess assumptions and issues of the methods; and (3) present a generic framework that overcomes possible issues. Based on the overview of fifteen recent representative studies, two methods can be used to estimate absolute magnitudes of ΔQ^c and ΔQ^h, while another method can be used to distinguish relative magnitudes of ΔQ^c versus ΔQ^h only. Because the methods’ fundamental assumptions about baseline versus altered period, water storage change and deep groundwater loss, precipitation-runoff relationship, hysteresis influence of human activity, and record of time series can seldom be satisfied for many watersheds, it is more realistic and practical to distinguish relative effects than to estimate absolute magnitudes of ΔQ^c and ΔQ^h. Moreover, a generic framework was presented for gauged watersheds with negligible groundwater loss, aiming to avoid misuse of the methods in practice.     

Nitrogen isotopic geochemical characteristics of crude oils in several basins of China

Nitrogen occupies a high content in crust and in atmospheric circle. It is one of the main elements in organism and an important element in sedimentary circle. Although nitrogen is little in crude oil, to a cer-tain degree, it influences the physical and chemical properties of oil, such as viscosity and density[1]. In reservoir the nitrogen-bearing compounds can form ion bonds or hydrogen bonds with substances on rock and form van der Vaals’ force among moleculae so they affect and alter the …     

Variability of parameters of the F2-layer maximum in the quiet midlatitude ionosphere under low solar activity: 2. Strong fluctuations of critical frequency

This paper presents a qualitative analysis of the properties and particular examples of strong (10% < |δfoF2| < 30%) and very strong (|δfoF2| > 30%) fluctuations in the critical frequency of the F2 layer (foF2) of the quiet ionosphere at midlatitudes under low solar activity according to the Irkutsk station data for 2007–2008. It is found that strong day-to-day fluctuations in foF2 are mainly related to changes in thermospheric parameters, which have a nature of planetary waves and tides. Evidently, very strong day-to-day fluctuations in foF2 are caused by superposition of the effects in the ionosphere caused by changes in the thermospheric parameters and those related to a complex of processes of solar wind interaction with the magnetosphere, including the effects caused by the reversal of the vertical component of the solar wind magnetic field southwards. The increase in foF2 during nighttime hours in winter up to values typical for the daytime maximum in foF2 is the brightest example of very strong changes in foF2 in the quiet ionosphere.     

The effect of solar activity on the secular variation of the geomagnetic field in Romania

The 11-year solar cycle effect in the geomagnetic components H and Z is made clear for Surlari Observatory and 19 repeat stations for the interval 1952–1974. The correlation with Wolf number and its time derivative is discussed in terms of the effects of the external and induced current systems.The $\text{H}\text{?}$ data available for solar cycle 20 (1964–1976) were processed to give the geographical distribution of the secular variation impulse for epoch 1969.5 in Romania. It is suggested that this distribution might reflect the deep internal structure of the area considered.A qualitative correlation is noted between long-period solar activity and variation of the horizontal component of the geomagnetic field at some repeat stations.     

Relationship between differential retention of Escherichia coli and Enterococcus faecalis and variations in enzyme activity in the scallop Patinopecten yessoensis

Uptake of Escherichia coli and Enterococcus faecalis and variations of trypsin amylase activity acid phosphatase and superoxide dismutase in tissue of the scallop Patinopecten yessoensis were detected. The results showed that P. yessoensis accumulated E. faecalis in larger numbers and more rapidly than E. coli, both with the highest concentration in the digestive tract and lowest in hemolymph. Compared to E. coli, all scallops exposed to E. faecalis showed significantly higher trypsin and AMS activity. SOD activity in hemocytes and ACP activity in hemolymph was significantly higher in the treatments with 5 log₁₀CFU/ml E. coli than with E. faecalis. But no significant differences in ACP activity of P. yessoensis exposed to a 3 log₁₀CFU/ml inoculum of both bacteria were recorded. In conclusion, the mass retention of gut microflora in P. yessoensis is positively correlated with digestive enzymes activity and negatively correlated with ACP activity in the hemocyte.