Effects of stocking density on the growth and immunity of Atlantic salmon salmo salar reared in recirculating aquaculture system (RAS)

Atlantic salmon reared in recirculating aquaculture system (RAS) may lead to inappropriately high stocking density, because fish live in a limited space. Finding the suitable stocking density of Atlantic salmon reared in RAS is very important for RAS industry. In this paper, the influence of stocking density on growth and some stress related physiological factors were investigated to evaluate the effects of stocking density. The fish were reared for 220 days at five densities (A: 24 kg/m³; B: 21 kg/m³; C: 15 kg/m³; D: 9 kg/ m³ and E: 6 kg/m³ ). The results show that 30 kg/m³ might be the maximum density which RAS can afford in China. The stocking densities under 30 kg/m³ have no effect on mortality of Atlantic salmon reared in RAS. However, the specific growth rate (SGR), final weight and weight gain in the high density group were significantly lower than the lower density groups and middle density groups. Moreover, feed conversion rate (FCR) had a negative correlation with density. Plasma hormone T₃ and GH showed significant decrease with the increase of the stocking density of the experiment. Furthermore, thyroid hormone (T₃), GH (growth hormone) activities were decreased with stocking density increase. However, plasma cortisol, GOT (glutamic oxalacetic transaminase) and GPT (glutamic pyruvic transaminase) activities were increase with stocking density increase. And the stocking density has no effects on plasma lysozyme of Atlantic salmon reared in RAS. These investigations would also help devise efficient ways to rear adult Atlantic salmon in China and may, in a way, help spread salmon mariculture in China.

     

On spatiotemporal characteristics of sea fog occurrence over the Northern Atlantic from 1909 to 2008

In this paper, the International Comprehensive Ocean and Atmosphere Data Set(ICOADS) is utilized to investigate the horizontal distribution of sea fog occurrence frequency over the Northern Atlantic as well as the meteorological and oceanic conditions for sea fog formation. Sea fog over the Northern Atlantic mainly occurs over middle and high latitudes. Sea fog occurrence frequency over the western region of the Northern Atlantic is higher than that over the eastern region. The season for sea fog occurrence over the Northern Atlantic is generally from April to August. When sea fogs occur, the prevailing wind direction in the study area is from southerly to southwesterly and the favorable wind speed is around 8 m s-1. It is most favorable for the formation of sea fogs when sea surface temperature(SST) is 5℃ to 15℃. When SST is higher than 25℃, it is difficult for the air to get saturated, and there is almost no report of sea fog. When sea fogs form, the difference between sea surface temperature and air temperature is mainly-1 to 3℃, and the difference of 0℃ to 2℃ is the most favorable conditions for fog formation. There are two types of sea fogs prevailing in this region: advection cooling fog and advection evaporating fog.     

Impacts of Norwegian large carnivore management strategy on national grazing sector

Increasing populations of large carnivores are leading to tension and conflicts with livestock production, a situation that potentially might escalate.In Norway the objective of the large carnivore policy is two-folded: to ensure viable carnivore populations and to secure a sustainable grazing industry. The main instrument is zonation, with carnivore management zones(CMZs) prioritized for reproduction of the large carnivore species separated from other areas prioritized for grazing livestock. The objective of this paper is to describe current knowledge about the impact of the zoning management strategy on the grazing industry. This is done by documenting status and changes in sheep production, losses of livestock to predating carnivores,and the use of grazing areas inside and outside the CMZs. CMZs offering protection for lynx, wolverine,bear and wolf cover 55% of the Norwegian mainland.30% of the sheep and 50% of the Sami reindeer grazing areas are found inside the CMZs. Livestock(semi-domestic reindeer excluded) is using 59% of the available natural pasture areas outside the CMZs, but only 26% inside the CMZs. The lowest use of availablegrazing areas was found inside zones for wolves(12%)and brown bears(6%). Livestock in these zones are confined to fenced enclosures, mostly on the farm itself, or moved to pastures outside the management zone for summer grazing. Livestock losses increased in the affected regions during the period when carnivores were reestablished. Later, losses declined when CMZs were established and mitigation efforts were implemented in these zones. The bulk of sheep and reindeer killed by carnivores are now found in boundary areas within 50 km off the CMZs, where sheep are still grazing on open mountain and forest ranges. Therefore, instruments to protect livestock in areas close to the CMZs are also needed. The number of sheep declined inside the CMZs from 1999 to 2014,but increased outside the zones. The reduction in the absolute number of sheep in the CMZs is balanced by a similar increase outside, thus the total sheep production in Norway is maintained. We conclude that although of little consequence for the total food production in Norway, the economic and social impact of the large carnivore management strategy can be serious for local communities and individual farmers who are affected. There is a need for more exact carnivore population monitoring to quantify the carnivore pressure, better documentation of reindeer losses, and a clearer and stricter practicing of thezoning strategy. Increased involvement of social sciences is important in order to understand the human dimension of the carnivore conflicts.     

Effects of spectral composition,photoperiod and light intensity on the gonadal development of Atlantic salmon Salmo salar in recirculating aquaculture systems (RAS)

Artificial lighting regimes have been successfully used to inhibit sexual maturity of Atlantic salmon in confinement.However,when these operations are applied in commercial recirculating aquaculture systems(RAS) using standard lighting technology,sexual maturation is not suppressed.In this study,an L_9(3~3) orthogonal design was used to determine the effects of three factors(spectral composition,photoperiod,and light intensity) on the gonadal development of Atlantic salmon in RAS.We demonstrated that the photoperiod at the tested levels had a much greater effect on the gonadosomatic index and female Fulton condition factor than spectral composition and light intensity.The photoperiod had a significant effect on the secretion of sex steroids and melatonin(P0.05),and a short photoperiod delayed sex steroid and melatonin level increases.The three test factors had no significant effects on the survival rate,specific growth rate,relative weight gain,and male Fulton condition factor(P0.05).The optimum lighting levels in female and male Atlantic salmon were LD 8:16,455 nm(or 625 nm),8.60 W/m~2;and LD 8:16,8.60 W/m~2,455 nm respectively.These conditions not only delayed gonadal development,but also had no negative effects on Atlantic salmon growth in RAS.These results demonstrate that a combination of spectral composition,photoperiod and light intensity is effective at delaying the gonadal development of both male and female salmon in RAS.     

Degradation of High Mountain Ecosystems in Northern Europe

Data material of a long-term high mountain ecosystem research project was used to interpret the grazing impact of reindeers. In central Norway investigations were conducted to both, areas where reindeer grazing is excluded, and areas where intensive pasturing is present for a long period of time.The comparative analysis of grazing impact was based on similar environmental conditions. The results were transposed to northern Norway where dramatic overgrazing had been exceeding the carrying capacity. Using landscape ecological mappings, especially of vegetation and soils, the impact of reindeer grazing in different areas became obvious. Non-grazed lichen-dominated ecosystems of the snow-free locations functioned sensitively near the limit of organism survival. These localities were most influenced by grazing as they offer the winter forage to the reindeers. So, intensive grazing in central Norway led to landscape degradation by destruction of the vegetation and superinduced by soil erosion. Those features were comparable to the situation in northern Norway, where a broad-scale destruction of the environment combined with a depression of the altitudinal belts had occurred due to overgrazing. Functioning principles of intact high mountain systems were explained and used to interpret the environmental background for the understanding of degradation phenomena. Finally, the use of a new model calculating the carrying capacity of high mountain landscape was discussed.     

Mineralogical study of surface sediments in the western Arctic Ocean and their implications for material sources

Mineralogical analysis was performed on bulk sediments of 79 surface samples using X-ray diffraction. The analytical results, combined with data on ocean currents and the regional geological background, were used to investigate the mineral sources. Mineral assemblages in sediments and their distribution in the study area indicate that the material sources are complex. （1） Feldspar is abundant in the sediments of the middle Chukchi Sea near the Bering Strait, originating from sediments in the Anadyr River carried by the Anadyr Current. Sediments deposited on the western side of the Chukchi Sea are rich in feldspar. Compared with other areas, sediments in this region are rich in hornblende transported from volcanic and sedimentary rocks in Siberia by the Anadyr Stream and the Siberian Coastal Current. Sediments in the eastern Chukchi Sea are rich in quartz sourced from sediments of the Yukon and Kuskokwim rivers carried by the Alaska Coastal Current. Sediments in the northern Chukchi Sea are rich in quartz and carbonates from the Mackenzie River sediments. （2） Sediments of the southern and central Canada Basin contain little calcite and dolomite, mainly due to the small impact of the Beaufort Gyre carrying carbonates from the Canadian Arctic Islands. Compared with other areas, the mica content in the region is high, implying that the Laptev Sea is the main sediment source for the southern and central Canada Basin. In the other deep sea areas, calcite and dolomite levels are high caused by the input of large amounts of sediment carried by the Beaufort Gyre from the Canadian Arctic Islands （Banks and Victoria）. The Siberian Laptev Sea also provides small amounts of sediment for this region. Furthermore, the Atlantic mid-water contributes some fine-grained material to the entire deep western Arctic Ocean.     

扬马延微陆块构造特征及火山型被动陆缘远端带构造演化模式

随着全球油气勘探的不断深入,北大西洋极地逐渐成为油气勘探研究的前沿领域,而扬马延矿区勘探程度极低。基于中海油冰岛矿区新采集的地震及重磁资料,结合其他有关扬马延微陆块最新的研究资料,开展了扬马延微陆块的地层和构造特征分析,以及与共轭盆地的对比,建立了扬马延火山型被动陆缘远端带的构造演化模式。研究表明:位于北大西洋格陵兰与挪威之间海域的扬马延微陆块,与北大西洋两侧陆架盆地古生代-中生代地层具有共轭特征;构造呈NE-SE向展布,发育拆离断裂体系,与挪威西部陆架盆地中生界拆离断裂体系具有相似性;构造内部受岩浆侵入及喷出等强烈影响,发育向海倾斜反射层(SDR)及岩浆溢流相沉积。在上述研究基础上,探讨了扬马延微陆块与格陵兰古陆和波罗的海古陆拉断分离的构造演化过程,认为扬马延在古生代-中生代与格陵兰古陆和波罗的海古陆为一体,在经历了古生代-中生代陆内碰撞、弱伸展到陆内裂谷和陆内热沉降后,受北大西洋拉开影响,经历了古近纪和新近纪火山型被动陆缘远端带的形成演化过程,在55 Ma第一次洋中脊扩张期,与波罗的海古陆挪威陆缘盆地分离,在25 Ma第二次洋脊跃迁时期,新生洋脊扩张导致扬马延微陆块与格陵兰古陆分离,在沉积与构造上开始与北大西洋火山型被动陆缘盆地产生分异,最终扬马延微陆块成为孤立在洋壳上的一个"弃子"。本次关于扬马延微陆块的研究揭示了火山型被动陆缘远端带在岩浆活动、拆离断裂作用下,减薄-破裂的残余陆壳及内部新生洋壳的构造面貌及板块构造背景下的演化过程。      

Type and evolution of landscapes of Nansha Islands

ＴＹＰＥＡＮＤＥＶＯＬＵＴＩＯＮＯＦＬＡＮＤＳＣＡＰＥＳＯＦＮＡＮＳＨＡＩＳＬＡＮＤＳＺｈａｏＨｕａｎｔｉｎｇ（赵焕庭）ＳｏｕｔｈＣｈｉｎａＳｅａＩｎｓｔｉｔｕｔｅｏｆＯｃｅａｎｏｌｏｇｙ,ｔｈｅＣｈｉｎｅｓｅＡｃａｄｅｍｙｏｆＳｃｉｅｎｃｅｓ,Ｇｕ...     

Seasonal change of steric sea level in the GIN seas

The Greenland Sea,Iceland Sea,and Norwegian Sea (GIN seas) form the main channel connecting the Arctic Ocean with other Oceans,where significant water and energy exchange take place,and play an important role in global climate change.In this study steric sea level,associated with temperature and salinity,in the GIN seas is examined based on analysis of the monthly temperature and salinity fields from Polar science center Hydrographic Climatology (PHC3.0).A method proposed by Tabata et al.is used to calculate steric sea level,in which,steric sea level change due to thermal expansion and haline contraction is termed as the thermosteric component (TC) and the halosteric component (SC),recpectively.Total steric sea level (TSSL) change is the sum of TC and SC.The study shows that SC is making more contributions than TC to the seasonal change of TSSL in the Greenland Sea,whereas TC contributes more in the Norwegian and the Iceland Seas.Annual variation of TSSL is larger than 50 mm over most regions of the GIN Seas,and can be larger than 200 mm at some locations such as 308 mm at 76.5 N,12.5 E and 246 mm at 77.5 N,17.5 W.     

Type and evolution of landscapes of Nansha Islands

Landscapes of the Nansha Islands may be divided into five types: tropical marine organism-breeding landscape of reef knoll like Zengmu Shoal, Wan’an Bank and so on; tropical marine organism-breeding landscape of atoll including all emerged reefs and most submerged reefs; tropical evergreen arbor-bush forest and phosphorous lime soil landscape of limesand Islets like Taiping Islet, Nanwei islet and so on; tropical shallow sea marine organism-breeding landscape of southern continental shelf; tropical oceanic and deep-sea marine organism landscape in middle and north parts of the Nansha Islands area. These five landscape types may be also summed up as two categories, one is tropical sea landscape including those in shallow and deep sea, the other is tropical coral reef landscape including those of reef knoll, atoll and limesand islet. This paper outlines the evolutional model of landscapes of the Nansha Islands. The distribution and evolution of landscape types are related with palaeogeography and modern environmental conditions. The former shallow sea of the Nansha islands was coastal zone in the Late Pleistocene epoch. Deep sea is evolved from shallow sea due to long and slow subsidence of crust. Modern coral reefs develop on old reef top of the Late pleistocene epoch or on baserock of continental shelf in the Holocene due to the rise of sea level in postglacial. Limesand islet is in the peak of developmental stage.     

Clean and safe supply of fish and shellfish to clear the HACCP regulation by use of clean and cold deep ocean water in Rausu, Hokkaido, Japan

For the supply of fish and shellfish to consumers in fresh condition, clean handling after catch from the sea is essential. According to HACCP （Hazard Analysis and Critical Control Points）, it is important to meet such requirement by keeping fish and shellfish under a certain low temperature and clean conditions after catching. The deep ocean water （DOW） characterized by low temperature and cleanliness has been chosen for fish and shellfish handlings, particularly for salmon, cod, and sea urchin in Town ＇Rausu＇ in Hokkaido, Japan. DOW below 2.9℃ of an amount of nearly 5 000 m^3 is planned to be pumped up every day from a depth of about 350 m, and temporarily stored in a large simulated tank on land. DOW is then supplied to fish boats through hydrants distributed throughout the harbor and used for keeping salmon in clean and cold conditions. Ice made from DOW is also used for lowering temperature if necessary. DOW and ice made from DOW are also used during the transportation of fish and shellfish. The entire system is scheduled to be completed by the summer of 2005.     

Effects of grazing exclusion on soil carbon and nitrogen storage in semi-arid grassland in Inner Mongolia,China

The semi-arid grasslands in Inner Mongolia, China have been degraded by long-term grazing. A series of ecological restoration strategies have been implemented to improve grassland service. However, little is known about the effect of these ecological restoration practices on soil carbon and nitrogen storage. In this study, characteristics of vegetation and soil properties under continued grazing and exclusion of livestock for six years due to a nationwide conservation program—′Returning Grazing Lands to Grasslands(RGLG)′ were examined in semi-arid Hulun Buir grassland in Inner Mongolia, China. The results show that removal of grazing for six years resulted in a significant recovery in vegetation with higher above and below-ground biomass, but a lower soil bulk density and pH value. After six years of grazing exclusion, soil organic C and total N storage increased by 13.9% and 17.1%, respectively, which could be partly explained by decreased loss and increased input of C and N to soil. The effects of grazing exclusion on soil C and N concentration and storage primarily occurred in the upper soil depths. The results indicate that removal of grazing pressure within the RGLG program was an effective restoration approach to control grassland degradation in this region. However, more comprehensive studies are needed to evaluate the effectiveness of the RGLG program and to improve the management strategies for grassland restoration in this area.     

Comparative study of the nutritional composition and toxic elements of farmed and wild Chanodichthys mongolicus

Information of the difference in quality between farmed and wild fish is central to better ensuring fish products produced in aquaculture meet regulatory and consumer requirements.Proximate composition,amino acid and fatty acid profiles,and toxic elements contents of farmed and wild Chanodichthys mongolicus were established and compared.Significantly higher crude protein content while lower moisture content in farmed fish compared to wild fish were observed(P0.05).The percentages of total amino acids(TAA),total essential amino acids(TEAA),total non-essential amino acids(TNEAA)and total delicious amino acids(TDAA) in farmed fish were all significantly higher than those in the wild equivalent(PO.05).Compared to the FAO/WHO recommended reference values,the ratios of TEAA/TAA(39.84%-40.33%) were comparable to 40%and TEAA/TNEAA(66.22%-67.60%) were above 60%.Fatty acid profiles in both fanned and wild C.mongolicus were dominated by monounsaturated fatty acid(MUFA),with farmed fish contained much more MUFA content compared to wild counterpart(P0.05).Notably,wild fish exhibited significantly higher levels of total polyunsaturated fatty acid(PUFA) including eicosapentaenoic acid(EPA) and docosahexaenoic acid(DHA) than farmed fish(P0.05).The EPA(C_(20:5n3))and linoleic acid(C_(18:2n6)) were the predominant PUFA in wild and fanned C.mongolicus,respectively.Moreover,farmed fish displayed an overall lower toxic element levels(As,Cd,Pb and Hg) in comparison with wild fish,and both were far lower than the established limit standard.In conclusion,our results suggest that the nutritional quality of farmed C.mongolicus was inferior to their wild counterpart with respect to fatty acids nutrition,and therefore further studies should focus on the improving C.mongolicus diet in order to enhance the overall nutritional composition.     

THREE-DIMENSIONAL BAROCLINIC NUMERICAL SIMULATION OF THE SOUTH CHINA SEA CIRCULATION＇S SEASONAL CHARACTERISTICS II. MIDDLE AND DEEP CIRCULATION

A three-dimensional baroclinic shelf sea model‘ s numerical simulation of the South China Sea (SCS) middle and deep layer circulation structure showed that: 1. In the SCS middle and deep layer, a seulhward boundary current exists along the east shore of the Indo-China Peninsula all year long.A cyclonic eddy (gyre) is formed by the current in the above sea areas except in the middle layer in spring, when an anticyclonic eddy exists on the eastern side of the current. In the deep layer, a larges-cale anticyclonic eddy often exists in the sea areas between the Zhongsha Islands and west shore of southern Luzon Island. 2. In the middle layer in snmmer and autumn, and in the deep layer in autumn and winter, there is an anticyclonic eddy (gyre) in the northeastern SCS, while in the middle layer in winter and spring, and in the deep layer in spring and snmmer, there is a cyclonic one. 3. In the middle layer,there is a weak northeastward current in the Nansha Trough in spring and snmmer, while in autumn and winter it evolves inl~ an anticyclonic eddy ( gyre), which then spreads westward l~ the whole western Nansha Islands sea areas.     

Diatom assemblages in surface sediments from the South China Sea as environmental indicators

We studied diatom distribution from 62 samples from the uppermost 1 cm of sediment in the South China Sea (SCS), using grabs or box corers in three cruises between 2001–2007. Fifty six genera, 256 species and their varieties were identified. Dominating species included Coscinodiscus africanus, Coscinodiscus nodulifer, Cyclotella stylorum, Hemidiscus cuneiformis, Melosira sulcata, Nitzschia marina, Roperia tesselata, Thalassionema nitzschioides, Thalassiosira excentrica, and Thalassiothrix longissima. Most surface sediments in the SCS were rich in diatoms, except for a few coarse samples. Average diatom abundance in the study area was 104 607 valve/g. In terms of the abundance, ecology, and spatial distribution, seven diatom zones (Zones 1–7) were recognized. Zone 1 (northern continental shelf) is affected by warm currents, SCS northern branch of the Kuroshio, and northern coastal currents; Zone 2 (northwestern continental shelf) is affected by intense coastal currents; Zone 3 (Xisha Islands sea area) is a bathyal environment with transitional water masses; Zone 4 (sea basin) is a bathyal-to-deep sea with stable and uniform central water masses in a semi-enclosed marginal sea; Zone 5 (Nansha Islands marine area) is a pelagic environment with relatively high surface temperature; Zone 6 (northern Sunda Shelf) is a tropical shelf environment; and Zone 7 (northern Kalimantan Island shelf area) is affected by warm waters from the Indian Ocean and coastal waters. The data indicate that these diatom zones are closely related to topography, hydrodynamics, temperature, nutrients and especially the salinity. Better understanding of the relationship between diatom distribution and the oceanographic factors would help in the reconstruction of the SCS in the past.     

Modeling seasonal variations of ocean and sea ice circulation in the Beaufort and Chukchi Seas： A model-data fusion study

A 3.8-kin Coupled Ice-Ocean Model （C1OM） was implemented to successfully reproduce many observed phenomena in the Beaufort and Chukchi seas, including the Bering-inflow-originated coastal current that splits into three branches： Alaska Coastal Water （ACW） , Central Channel, and Herald Valley branches. Other modeled phenomena include the Beaufort Slope Current （BSC） , the Beaufort Gyre, the East Siberian Current （ ESC）, mesoscale eddies, seasonal landfast ice, sea ice ridging, shear, and deformation. Many of these downscaling processes can only be captured by using a high-resolution CIOM, nested in a global climate model. The seasonal cycles for sea ice concentration, thickness, velocity, and other variables are well reproduced with Solid validation by satellite measurements. The seasonal cycles for upper ocean dynamics and thermodynamics are also well reproduced, which include the formation of the cold saline layer due to the injection of salt during sea ice formation, the BSC, and the subsurface upwelling in winter that brings up warm, even more saline Atlantic Water along the shelfbreak and shelf along the Beaufort coast.     

Impacts of SIS and CICE as Sea Ice Components in BCC_CSM on the Simulation of the Arctic Climate

Sea ice is a sensitive indicator of climate change and an important component of climate system models. The Los Alamos Sea Ice Model 5.0(CICE5.0) was introduced to the Beijing Climate Center Climate System Model(BCC_CSM) as a new alternative to the Sea Ice Simulator(SIS). The principal purpose of this paper is to analyze the impacts of these two sea ice components on simulations of basic Arctic sea ice, atmosphere, and ocean states. Two sets of experiments were conducted with the same configurations except for the sea ice component used, i.e., SIS and CICE. The distributions of sea ice concentration and thickness reproduced by the CICE simulations in both March and September were closer to actual observations than those reproduced by SIS simulations, which presented a very thin sea ice cover in September. Changes in sea ice conditions also brought about corresponding modifications to the atmosphere and ocean circulation. CICE simulations showed higher agreement with the reference datasets than did SIS simulations for surface air temperature, sea level pressure, and sea surface temperature in most parts of the Arctic Ocean. More importantly, compared with simulations with SIS, BCC_CSM with CICE revealed stronger Atlantic meridional overturning circulation(AMOC), which is more consistent with actual observations. Thus, CICE shows better performance than SIS in BCC_ CSM. However, both components demonstrate a number of common weaknesses, such as overestimation of the sea ice cover in winter, especially in the Nordic Sea and the Sea of Okhotsk. Additional studies and improvements are necessary to develop these components further.     

北半球海冰和陆雪对东亚季风的影响及作用的可能途径

本文应用统计方法分析陆雪和海冰与东亚夏季风的关系。分析结果表明:前期海冰和陆雪,对夏季风强度有影响,而与夏季风同时的海冰和陆雪的异常,却与夏季风相关甚小,这是由于大气状况的变化与下垫面的能量储放有关。本文初步探讨北极海冰对东亚夏季风影响的可能途径,认为海冰通过大西洋海温、大西洋副热带高压及青藏高压,由夏季对流层上层的东西热力环流圈和季风环流圈,对东亚夏季风起一定影响。     

Colonization and Probiotic Effect of Metschnikowia sp. C14 in the Intestine of Juvenile Sea Cucumber,Apostichopus japonicus

Viable cell count was used to determine whether Metschnikowia sp.C14 can colonize the intestine of juvenile sea cucumber Apostichopus japonicus.Sea cucumber individuals were divided into two groups,which were fed the control diet for 38 days or the C14-supplemented diet at 105 cells g−1 diet for 28 days,then the control diet from day 29 to day 38.The number of C14 cells in the intestine of sea cucumber fed the C14-supplemented diet significantly increased from day 7 to day 28,and decreased from day 29 to day 38.Sea cucumber fed with the diet containing C14 showed a significant increase in trypsin activity and lipase activity from day 21 to day 33 compared with the control.Feeding C14 significantly improved the phagocytic activity and respiratory burst in coelomocytes from day 21 to day 35 and from day 14 to day 38,respectively.In addition,there was an obvious enhancement in lysozyme activity(from day 21 to day 38 or day 33),phenoloxidase activity(from day 21 to day 28)and total nitric oxide synthase activity(from day 14 to day 38)in coelomic fluid supernatant and/or coelomocyte cell lysate supernatant compared with the control.There were significant positive correlations between the number of C14 cells colonizing the intestine and trypsin activity of the intestine,lysozyme activity of the coelomic fluid supernatant and coelomocyte lysate supernatant from sea cucumber.These data suggested that the number of C14 cells should be maintained at 105 cfu(colony-forming units)g−1 intestine material for the maximum benefit.     

Dominant modes of geopotential height in the northern hemisphere in summer on interdecadal timescales

Empirical orthogonal function(EOF) analysis is performed on the field of the northern hemisphere geopotential height at 200-hPa using a 54-year(1958-2011) record of summer data on an interdecadal time scale.The first dominant mode,which shows smooth semi-hemispheric variation with maximum action centers in the western hemisphere in the mid-latitudes over the eastern Pacific,North America,and the North Atlantic,is related to global warming.The second mode,which has a pronounced tropical-extratropical alternating pattern with active centers located over the eastern hemisphere from Western Europe across East Asia to the western Pacific,has a close relationship with the Arctic Oscillation.Further analysis results indicate that the two dominant modes show good correlation with the Arctic sea ice concentration(SIC),with correlation coefficients between these two modes and the first two EOF modes of the Arctic SIC reaching 0.88 and 0.86,respectively.