Organic components in size-separated aerosols from the western North Pacific

Marine aerosols in the western North Pacific were collected using a cascade impactor. Size-separated aerosols were analyzed for organic carbon, alkanes and polycyclic aromatic hydrocarbons (PAH). The results showed that the rate of decrease of the atmospheric concentrations of these organic components with increase in distance from Japan as well as from the coast of the Eurasian Continent was in the order PAHalkanes>organic carbon. The bulk of all these organic components occurred in the smallest size fraction of particles (<1m). Analysis of the alkanes and PAH indicated that the hydrocarbons in aerosols in Japanese coastal marine areas are primarily derived from terrestrial anthropogenic sources which also contribute to a lesser extent to aerosols in marine areas about 1,000 km off the coast of Japan. In remote marine areas the hydrocarbons on small particles (<1m) have principally a natural terrestrial origin while those on larger particles are marine in origin.     

The content and the oxidation state of arsenic and antimony in coastal water of Japan

The content and the oxidation state of arsenic and antimony in coastal seawater were determined coupled with chemical separation and neutron activation method. The contents of arsenic and antimony in coastal seawater were estimated to be 1.56–2.73g As/l, 0.25–0.57g Sb/l. Most parts of these elements exist as soluble ionic species, passing through 0.45 pore-size membrane filter, and at surface, 80–97% of arsenic occurs as arsenate and 70–94 % of antimony may occurs as antimonate. The vertical distribution of arsenite and arsenate is almost constant from the surface to 1,200 m depth. However the distribution of Sb(III) and Sb(V) is much more complicated with depth.     

Distribution of particulate organic carbon and nitrogen in the Bering Sea and northern North Pacific Ocean

Particulate matter was collected in the Bering Sea and the northern North Pacific Ocean during the cruise of R. V. Hakuho-maru, Ocean Research Institute of Tokyo University in summer of 1975. The particulate matter was analyzed for organic carbon and nitrogen, chlorophylla and amino acids.The concentrations of particulate organic carbon and nitrogen were measured with the range of 16–422gC l^–1 and 1–85gN l^–1, 19–186gC l^–1 and 1–26gN l^–1, 46–1,038gC l^–1 and 6–79gN l^–1 and 19–246gC l^–1 and 2–25gN l^–1 in the Oyashio, the Deep Bering Sea, the continental shelf of Bering Sea and the northern North Pacific, respectively. Particulate organic carbon and nitrogen decreased with depth throughout the areas. The average concentrations of organic carbon and nitrogen in the entire water column tended to decrease in the following order; the continental shelf > Oyashio > northern North Pacific > Deep Bering Sea.C/N of particulate matter varied in the range of 3–15 (7 on average) in surface waters throughout the areas and these values tended to increase with depth to 5–20 (11 on average) in deep waters without significant regional variability.Linear regressions between chlorophylla and particulate organic carbon in the euphotic layers indicate that detrital organic carbon accounted for 34.2, 44.9, 49.1 and 25.2 % of particulate organic carbon in the Oyashio, the Deep Bering Sea, the continental shelf and the northern North Pacific, respectively.Particulate amino acid was determined in the range of 10.3–78.0g l^–1, 104–156g l^–1 and 10.4–96.4g l^–1 in the Deep Bering Sea, the continental shelf and the northern North Pacific, respectively. Aspartic acid, glutamic acid, serine, glycine and alanine were found as dominant species of amino acid of particulate matter.     

Vertical distribution of heavy metals and its seasonal variations in Beppu Bay

From July to November, the thermocline which has strong temperature gradient (0.7C m^–1) is formed in the bottom water of Beppu Bay, and it prevents the downward mixing of surface water. This has caused the bottom water of the basin to become depleted in oxygen, and in November the bottom water below about 60 m depth becomes anoxic. Accordingly manganese and iron are reduced and more soluble under the anoxic condition, those concentrations are high relative to surface water, and the maximums are 1,240g l^–1 and 80g l^–1. Under the anoxic condition, the flux of dissolved manganese from the sediment is about 10g cm^–2 day^–1.     

Ammonium uptake kinetics and interactions between nitrate and ammonium uptake inChattonella antiqua

Ammonium uptake kinetics and interactions between nitrate and ammonium uptake were examined inChattonella antiqua. After the addition of ammonium to the culture ofC. antiqua, the ammonium concentration decreased linearly with time. The ammonium uptake rate as a function of ammonium concentration followed the Michaelis-Menten equation; the maximal uptake rate was 2.0 pmol cell^–1hr^–1 and the half saturation constant, 2.2M. Although the ammonium uptake was not affected by nitrate, uptake of nitrate was rapidly (15min) suppressed by ammonium and a 50% reduction in nitrate uptake was observed at an ammonium concentration ofca. 2M.     

A field study of physico-chemical states of artificial radionuclides in seawater

The physico-chemical states of artificial radionuclides,⁹⁰Sr,¹³⁷Cs and¹⁴⁴Ce in seawater were investigated by radiochemical analysis of filtered and unfiltered seawater. The difference of radionuclide concentrations between unfiltered and filtered seawaters was defined as the particulate form radioisotope and its particle ratio was discussed.Practically no particulate⁹⁰Sr, greater than 0.22 in size, was observed in both coastal and open seawaters, but some of¹³⁷Cs seemed to be insoluble in some circumstances, especially in coastal waters. A considerable amount of¹⁴⁴Ce was found to be particulate.An estimation of the radionuclides in particulate form was made for Kashima-nada seawaters collected in 1970 to 1972, and it was shown that the possible occurrence of particulate radionuclides, greater than 0.22 in size, were 1% or less for⁹⁰Sr and 6% for¹³⁷Cs. In the coastal water, 80 % of¹⁴⁴Ce were seemed to be in particulate form, but in the open seawater only a few%. The influences of suspended materials to¹³⁷Cs and¹⁴⁴Ce concentration levels in seawater were not negligible and further investigations are desirable.     

Rates of net assimilation and respiration of amino acids by marine bacteria

The selectivity of amino acid assimilation by marine bacteria was examined using seven kinds of¹⁴C-amino acids and the acid hydrolysate of¹⁴C-labelled proteins. It was found that the net assimilation and respiration by marine bacteria followed MICHAELIS-MENTEN kinetics for all of amino acids used in our experiments. Maximum velocities of amino acids were 0.01 to 0.19g carbon/hour per 2×10⁷ cells for net assimilation and less than 0.18g carbon/hour per 2×10⁷ cells for respiration at 20C. The velocity of gross assimilation was found with the following order: phenylalanine>valine, glutamic acid>serine, arginine>tryptophan>glycine. The assimilation velocities of amino acids in these laboratory works showed almost the same order as those in field experiments. The assimilation velocity of an amino acid was influenced by coexisting another amino acids or glucose. The assimilation velocity in lower substrate range of amino acids was directly proportional to the number of bacterial cells in the range from 6×10² to 3×10⁴ cells per ml. No linear relation between the assimilation velocity of amino acids and reciprocal of absolute temperature was found, but a marked bending was observed at 15 to 20C. The velocity at the optimum temperature was three to six times of that at 5C.     

Observations of upwelling around the Izu Peninsula,Japan: May 1982

Hydrographic observations between the Izu Peninsula and Oshima Island, Japan, in May 1982, showed upwelling around the tip of the Peninsula and possibly also in the lee of Oshima Island. The upwelling introduced water as cold as 18 C with nitrate concentrations of 3M to the surface. Temperature/salinity analyses indicated that the upwelled water was of Kuroshio characteristics. Slightly fresher water was advected out of Sagami Bay in a coastal counter current.Contribution number 470 from the Shimoda Marine Research Center, University of Tsukuba.     

Differential sorption of organic matter by various sized sediment particles in recent sediment from the bering sea

A recent unconsolidated marine sediment collected from the Aleutian basin of the Bering Sea (5702.9N, 17657.4W) was separated into eight size fractions by sieving. Each of the fractions was analyzed for density, organic carbon, total nitrogen and various organic materials. A detailed analysis of the correlation of organic carbon and total nitrogen to the mean diameter of the particles separated, indicated that the surface sorption processes of organic matter were the determinative factor controlling the concentration of organic carbon and total nitrogen on silt and clay particles of the sediments.Selective sorption of amino acids was found to occur in the 8–20 and 5–8m. fractions of the sediment. This is assumed to be due to their surface active property.     

Sterols of the suspended matters in sea water

The present study deals with the content and composition of sterols of the marine-suspended matter in the surface sea water of Kagoshima-Bay. The suspended matters were separated into 3 fractions by filtration with the glass and millipore filters. The sterols in each fraction were analyzed by gas-liquid chromatography on 1.5 % SE-30. The results obtained are as follows: 1) The sterol contents ranged from 2.3 to 20.5g/liter of sea water. 2) Cholesterol was usually present as a predominant sterol in both suspended and dissolved matters. 3) A relatively large amount of other sterols such as 22-dehydro-cholesterol, campesterol, stigmasterol,-sitosterol, and fucosterol were occasionally detected.     

Respiration rates of copepod larvae and a ciliate from a tropical sea

The respiration rates of copepod larvae and a ciliate (Placus sp.) from a tropical sea were measured with an oxygen electrode method. The general range of body size of these animals was 40–161m (diameter equivalent to a sphere), and the respiration rate measured was 0.00076–0.00176l O₂ (animal)^–1h^–1 (or 2.02–9.05l O₂ (mg wet weight)^–1h^–1) at 25.5–29.2C. There was no marked difference observed between the respiration rates of copepod larvae and the ciliate. The respiration rates obtained here are relatively higher than the rates of other similar sized protozoans found in the literature, but lower than the rate extrapolated from larger planktonic copepods in tropical seas.The present results and other information available on microzooplankton biomass suggest that microzooplankton respiration is of near equal importance to that of net zooplankton in the study of energy flow through tropical, pelagic ecosystems.     

Calcium-alkalinity relationship in the North Pacific

The dissolution of calcium carbonate in deep ocean water causes variation in calcium concentration (Ca) and alkalinity (TA) in the ratio of one to two. The decomposition of organic matter generates nitric acid, phosphoric acid and sulfuric acid. A proton flux which is derived from this process also changes alkalinity. Using the variation in nitrate concentration (NO₃) as an index of the proton flux, the relationship betweenCa,TA andNO₃ is expressed asCa=0.5TA+0.63NO₃ The values of Ca obtained from direct measurements in the North Pacific are in good agreement with the values estimated from this equation.     

Benzo(a)pyrene in the sediment of Osaka Bay and Keihin Canal and its estimated sources

Benzo(a)pyrene (BP) in the sediment of Osaka Bay were determined. High concentration was found at two stations near the mouth of Shin-Yodo and off Kobe respectively (0.98g g^–1 dry mud basis, 1.1g g^–1). The concentration decreases regularly from the mouth of the river further into the bay. The other supplemental determination was carried on the sediment and the seawater of Keihin Canal. Notable BP concentration of 89g g^–1 was observed in the sediment beneath the effluent outlet of a gasworks. The BP in the sediment near the ironworks was also considerable, while the BP was relatively less in the sediment beneath the effluent outlet of refineries. These data suggest that coke plants working in gasworks or ironworks may be the larger sources of BP than refineries. The results of Osaka Bay coincide with this hypothesis.Cadmium, lead, copper, zinc, nickel, ignition loss and fine sand content were measured from the same sediment samples of Osaka Bay. Cd, Pb, Cu and ignition loss showed the regular distributions which resemble to BP and accordingly, good correlations with BP. No significant correlations were found between BP and Ni, Zn and fine sand content which showed no regular distributions.     

Mean Vertical Distribution of the Dissipation Rate of Turbulent Energy in the Black Sea. Comparison with the Existing Models

We compare the results obtained by using theoretical and semiempirical models developed for the evaluation of the dissipation rate of turbulent energy in a stratified ocean with independent distribution of this quantity established by the authors for the active layer of the Black Sea (50–300 m) by using a one-dimensional model taking into account the balance of heat, salt, and fluid inside the layer. It is shown that, in a layer with gradual variation of the Väisälä–Brunt frequency N as a function of depth, the predominant sink of the energy of motion into dissipation N ² is ensured by the flow of energy through the spectrum of internal waves toward low frequencies and small vertical scales. On the contrary, in layers with abrupt drops of density as a function of depth (layers with jumps of density), an important role is played by the interface-type waves and the dependence of on N transforms into N .     

Thermolability of dehydrogenases from a psychrophilic marine bacterium

The heat lability of succinic dehydrogenase, malic dehydrogenase and lactate dehydrogenase in cell-free extracts ofVibrio marinus MP-1 grown at 15C was compared for the tris buffer suspended enzymes and the NaCl suspended enzymes. The tris buffer suspended enzymes lost 50 percent activity at low thermal exposure between 5C and 27C. No activity was evident on thermal exposure between 32C and 39C. The enzyme extracts suspended in NaCl at 36 % were more heat stable, losing 50 percent activity after exposure between 16C and 40C. The enzymes lost all activity on thermal exposure between 33C and 42C in the presence of NaCl.     

Intrusive Stratification of the Gulf-Stream Frontal Zone According to the Results of Investigations Performed during Cruise 43 of the R/V Akademik Vernadskii

We analyze the data of investigation of the intrusive structure of the Gulf-Stream frontal zone obtained in making frequent drift sections with the help of an MGI-8102 probing complex, study the regularities of variation of temperature, salinity, and density along separate intrusions, and present a series of results connected with the specific features of initiation and development of intrusions and the types of exchange processes determining their transformations. It is shown that the T-diagrams of all intrusions are well separated into segments with different slopes. Moreover, by comparing the slopes and locations of these segments with each other and with the T-diagrams of the Gulf Stream and slope waters, we can fairly reliably attribute the corresponding segments of intrusions to one of the following four types: initial dynamic folds of the frontal zone, layers of domestic water separating the intrusive segments of foreign water from each other, intrusive segments characterized by the penetration of ambient water, and segments not intrusive initially but getting the required slope as a result of interaction with upper and lower intrusive segments. For segments of the last two types, it is possible to specify the predominant type of exchange.     

On nonlinear sea waves and the induced mean flow

The nonlinear modulation of water wave groups is investigated and the interaction equations with induced flows are obtained. The analysis is performed up to the third order of the wave steepness a by treating it as a small parameter in the singular perturbation technique by means of the Krylov-Bogoliubov-Mitropolski method. The equation which governs the development of the wave envelope is found by a modification of the ordinary nonlinear Schroedinger equation for the case of uniform depth. The equations governing the behavior of the induced mean flow are examined by deriving the second order flow when the form of the modulated wave train is prescribed. The present theory can describe the mean flow caused by the radiation stress. Some applications containing the monochromatic wave instability are given to confirm the theoretical results.An outline of this paper was presented at The Ocean Surface Symposium (Sendai, 1984).     

Some factors affecting the survival of coliform bacteria in seawater

Some experiments were carried out to explain thein situ phenomena that the number of coliform organisms decreased rapidly from estuaries to offshore, and also at deeper layer, and that the appearance of coliform types varied.In natural seawater, experimental results did not show thatEscherichia coli was extinct by self-purification or anti-biosis action of seawater, but it showed that this organism decreased mainly because of their starvation caused by lack of nourishment. Although the decreasing rate of bacterial density was delayed in enriched seawater, addition of nutrient even at the time of bacterial extinction promoted the appearance of variated form of this bacteria with floc formation.Flocculation of bacterial cells was influenced by quality and quantity of added nutrients. Temperature was shown to have an effect on the floc formation, but appearance of variated form in flocculated cells ofE. coli was not affected by temperature. Flocculated particles of coliform bacteria were adsorbed on suspended particles in seawater and precipitated rapidly. This phenomenon seems to be a cause of the rapid disappearance of coliform bacteria in coastal waters.In bottom deposits the coliform bacteria probably survive longer as physiologically variated forms when suitable nutrients were supplied.     

Alkylbenzenesulfonate (ABS) in the bottom muds of the inner part of Tokyo Bay

The contents of alkylbenzenesulfonate (ABS) in the bottom sediments were analyzed for the inner part of Tokyo Bay and five inflowing rivers. Methylene blue active substances (MBAS) was extracted from the dired sample with methanol-benzene mixture.After the concentration of MBAS was determined by colorimetry, the ratio ABS/MBAS was estimated by an infrared spectrum of MBAS-MB complex. The net content of ABS was calculated from these two values.The ABS contents in the surface of the bottom sediments of the rivers and sea ranged 1.959g/g and 014.1g/g dry weight, respectively. The contents of ABS in the river sediments reflected the degree of pollution of each river by urban waste waters. From the results of the analysis of the core samples of sea sediment at two points containing ABS only in the upper layer, the average rate of sedimentation of the bottom muds in the inner part of Tokyo Bay since the usage of ABS began was estimated as 3 cm/year and 7 cm/year respectively.     

Formation of the vertical salinity profile in the Black Sea

The vertical distribution of salts brought by the Bosphorus undercurrent is numerically evaluated. By multiplying the average vertical salinity gradient by the diffusion coefficient,K _z , and the cross-section of the sea at the appropriate depth, we can determine the total vertical salt flux,Q(z). The derivative ofQ with respect toz depicts the salt source intensity distribution over depth. The highest intensity, Q/z, matches the 200 m depth level, i.e. the shelf edge. Below 1500 m, Q/z equals merely 0.1% of the value observed at a depth of 200 m. Above 37 m, salts are noted to sink, which corresponds to their outflow with the Bosphorus current. The distribution of Q/z and the respective values of mineral phosphorus and hydrogen sulphide are matched up.Translated by Vladimir A. Puchkin.