4种微量金属元素对绿色巴夫藻生长、叶绿素a及大小的影响

把4种微量金属离子以不同的浓度添加到绿色巴夫藻的培养液中，结果表明：Hg^2 和Pb^2 浓度分别在5μg/L和200μg/L时，对绿色巴夫藻的生长有较好的促进作用，同时也使叶绿素a的含量提高；Hg^2≥20μg/L时，绿色巴夫藻的生长受到抑制，叶绿素a的含量降低。Pb^2 所有的试验浓度都使绿色巴夫藻生长加快，叶绿素a的含量提高；C0^2 在1μg/L时对绿色巴夫藻的生长有最好的促进效果，但当C0^2 ≥5μg/L时，开始对生长出现抑制作用，而C0^2 所有的试验浓度都使叶绿素a的含量降低；Mn^2 浓度在100μg/L时，对促进绿色巴夫藻生长的效果最佳，400μg/L的浓度开始对生长出现抑制作用，但所有Mn^2 的试验浓度对叶绿素a含量的变化影响不显著。4种微量金属元素都使绿色巴夫藻略微变小。     

植物生长调节剂对绿色巴夫藻生长的影响

研究了二种植物生长调节剂对绿色巴夫藻 ( Pavlova viridis)的生长、叶绿素含量和干重的影响。结果表明 ,在试验浓度范围内 ,2 ,4— D对绿色巴夫藻生长的影响在培养早期比后期明显 ,最适浓度为 0 .1 m g/L ;6-BA质量浓度≥ 1 mg/L时对绿色巴夫藻有致死作用 ,质量浓度为 0 .1～ 0 .8mg/L时 ,对绿色巴夫藻的生长有促进作用 ,促进生长的最适质量浓度为 0 .5mg/L。在一定浓度范围内 ,二种植物生长调节剂都可促进绿色巴夫藻的生长 ,提高叶绿素含量 ,增加试验藻的干重。     

植物生长调节剂对绿色夫藻生长的影响

研究了二种植物生长调节剂对绿色巴夫藻（Ｐａｖｌｏｖａ ｖｉｒｉｄｉｓ）的生长、叶绿素含量和干重的影响。结果表明,在试验浓度范围内,２,４－Ｄ对绿色巴夫藻生长的影响在培养早期比后期明显,最适浓度为０．１ｍｇ／Ｌ;６－ＢＡ质量浓度≥１ｍｇ／Ｌ时对绿色巴夫藻有致死作用,质量浓度为０．１～０．８ｍｇ／Ｌ时,对绿色巴夫藻的生长有促进作用,促进生长的最适质量浓度０．５ｍｇ／Ｌ。在一定浓度范围内,二种植物生长调节剂都可促使绿色巴夫藻的生长,提高叶绿素含量,增加试验藻的干重。     

温度、照度和氮磷营养盐对长茎葡萄蕨藻富硒的影响

【目的】探索富硒长茎葡萄蕨藻(Caulerpalentillifera)的培养条件。【方法】以亚硒酸钠(Na_2SeO_3)为硒源,采用单因素实验,研究不同硒浓度对长茎葡萄蕨藻的生长、光合色素和硒富集量的影响,通过正交实验,找出适宜长茎葡萄蕨藻富硒的最佳温度、照度和氮磷比组合条件。【结果】在亚硒酸钠0、0.5、1.0、2.0、4.0、8.0mg/L条件下,随亚硒酸钠添加量增加,藻体硒质量分数(干基)从1.70μg/g增加至50.10μg/g,且各组间差异均有统计学意义(P<0.05);亚硒酸钠添加量2.0 mg/L时,藻体硒质量分数达到11.7μg/g;添加量大于2.0 mg/L时,显著抑制藻体的生长(P<0.05),藻体叶绿素a和叶绿素b含量显著性降低(P<0.05);添加量低于2.0 mg/L时,藻体的生长、叶绿素a和叶绿素b含量均无显著性差异;类胡萝卜素含量各组间无明显变化。在培养液亚硒酸钠质量浓度为2.0 mg/L的条件下,藻体最适的生长条件组合是温度28℃、照度2 000 lx、氮磷比10∶1,藻体的最优富硒条件是温度24℃、照度2 000 lx、氮磷比8∶1。【结论】长茎葡萄蕨藻在开发含硒安全性功能海藻食品方面有较大应用潜力。     

TraceeElements in Leccinum scabrum mushrooms and topsoils from Kłodzka Dale in Sudety Mountains, Poland

In the current study, we determined concentrations and transfer rates of Ag, Al, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, P, Rb, Sr and Zn to Brown Birch Scaber Stalks (Leccinum scabrum) mushrooms emerged in the area of Sudety Mountains (Sudetes) in Poland. Fruiting bodies and topsoil samples beneath L. scabrum were collected form the K odzka Dale. The trace elements were determined using validated method and inductively coupled plasma - atomic emission spectroscopy (ICP-AES) for final measurement. Mushrooms contained Ag, Cr, Hg, Co, Ni and Sr at < 1.0 μg/g dry weight; Ba and Pb at ~1.0 μg/g dw; Cd at < 5 μg/g dw; Cu and Mn at > 10 μg/g dw; Al and Ca at ~100 μg/g dw; Fe, Na, Rb and Zn at 100 to 500 μg/g dw, Mg at ~1,000 μg/g dw; P at ~5,000 μg/g dw and K at ~30,000 μg/g dw. Ca, Mn and Ni were nearly equally distributed between stipes and caps; stipes compared to caps were enriched in Ba, Na and Sr, while caps were enriched in Ag, Al, Cd, Co, Cr, Cu, Fe, K, P, Pb, Rb and Zn. The values of bioconcentration factor (BCF) varied highly depending on chemical element and were >1 for Ag, Cd, Cu, K, Mg, Na, P, Rb and Zn, while <1 for Al, Ba, Ca, Co, Cr, Fe, Mn, Ni, Pb and Sr. Topsoil showed elevated content of lead and mean concentration was 99 ± 32 μg/g dw, while cadmium was at 0.41 ± 0.15 and those two highly toxic to human elements occurred in edible caps of L. scabrum at 4.5 ± 2.2 and 2.9 ± 2.0 μg/g dw, respectively.     

温度、光照对小环藻生长和叶绿素a含量的影响

用单因子和正交实验方法研究了温度和光照对小环藻生长和叶绿素a含量的影响。结果表明,温度对小环藻生长和叶绿素a含量有极显著影响(P<0.01),当温度在30~40℃时比增长率和叶绿素a含量分别为0.37~0.38 d-1和1 084.4~1 214.2μg.L-1,均显著高于其他温度组。光照对小环藻生长和叶绿素a含量有极显著影响(P<0.01),当光照强度从29.25~146.26μmol.m-2.s-1时比增长率最高,为0.30~0.32 d-1;而叶绿素a含量则在9.75~58.50μmol.m-2.s-1时最高,达709.7~731.8μg.L-1,极显著高于其他照度组。正交实验结果表明:在35℃、87.75μmol.m-2.s-1条件下种群增长最快,比增长率为0.4402 d-1;在30℃、29.25μmol.m-2.s-1时,叶绿素a含量最高,为1 746.6μg.L-1。     

氮源、磷源及重金属离子对绿色巴夫藻生长的影响

研究了不同氮源、磷盐、不同氮磷比以及培养液中3种重金属离子含量对绿色巴夫藻(Pavlovaviridis)生长的影响。结果表明:无机氮、有机氮共同提供氮源,以及磷酸二氢钠提供磷源,氮磷比5∶1时,绿色巴夫藻生长速度最快;铅、锌、铜离子可不同程度地影响绿色巴夫藻的生长,三者的毒性依次增大,三者的96hEC50分别为25.700、7 .079和1 469mg/L。     

波吉卵囊藻遗传转化体系选择标记的筛选

【目的】确定波吉卵囊藻(Oocystis borgei)遗传转化体系中的选择筛选标记。【方法】使用分光光度计法测定藻体生物量、叶绿素含量及观察藻液颜色变化,综合评定波吉卵囊藻对6种抗生素的敏感性。【结果】1)波吉卵囊藻对博来霉素非常敏感,25μg/m L的博来霉素即能完全抑制藻体生长,叶绿素含量极显著低于对照组(P0.01),藻体颜色发黄变白;2)红霉素和氯霉素各浓度的处理都会使藻体生物量和叶绿素含量极显著降低(P0.01),但是溶解红霉素和氯霉素的乙醇溶剂会对藻体生长产生抑制,不适用作筛选试剂;3)氨苄青霉素、遗传霉素和庆大霉素对波吉卵囊藻生长抑制作用不明显,甚至会促进叶绿素含量的增加。【结论】质量浓度为25μg/m L的博来霉素可以作为波吉卵囊藻遗传转化中的筛选试剂。     

汞离子对细基江蓠孢子萌发和生长的影响

研究了4种Hg2+浓度对细基江蓠果孢子和四分孢子附着、萌发和生长的影响。结果表明:与对照组相比,Hg2+抑制了果孢子附着却促进了四分孢子的附着,但0.0025 mg/L浓度与对照组的差异不显著(P>0.05),各浓度间差异显著(P<0.05);Hg2+在实验浓度范围内(0.0025、0.0050、0.0075、0.0100 mg/L)影响了孢子的正常萌发,使孢子不能正常分裂和生长,刺激了孢子脱落,并且使盘状体的形态发生变异;`Hg2+不同浓度之间也存在显著差异(P<0.05),并且Hg2+浓度越高对孢子的萌发和生长影响越大。     

ESTIMATION OF ATMOSPHERIC INPUT OF MERCURY TO SOUTH LAKE AND JINGYUE POOL

Taking South Lake and Jingyue Pool in Changchun as examples,we determined particulate Hg in ambient air and Hg concentration in precipitation.Results indicated that particulate Hg in air and Hg concentration in precipita-tion in both sites in nonheating period were lower than that in heating period,which is influenced by coal combustion.The annual particulate Hg concentrations in precipitation were 0.268μg/L(South Lake)and 0.108μg/L(Jingyue Pool).The high-er particulate Hg concentrations in precipitation were 0.268μg/L(South Lake) and 0.108μg/L(Jingyue Pool).The high-er particulate Hg concentration in air and Hg concentration in precipitation in South Lake than that of Jingyue Pool indicat-ed that mercury deposition was influenced by anthropogenic sources(especially from coas combustion).Hg concentration in precipitation is related with particulate Hg in the atmosphere.Dry deposition fluxes were estimated by using dry deposi-tion model on the base of particulate Hg concentrations in the atmosphere,they were estimated by using dry deposi-tion model on the base of particulate Hg concentrations in the atmosphere,they were 35.5μg/(m^2.a) for South lake and 15.3μg/(m^2.a)for Jingyue Pool.Combined with precipitation amount,wet deposition fluxes were estimated in these two sites,152.2μg(m^2.a)for South Lake and 61.2μg/(m^2.a)for Jingyue Pool.Atmospheric Hg deposition fluxes were 160.2g/a for South Lake and 328.62g/a for Jingyue Pool.e     

无单放货的表现形式及责任

无单放货频频发生 ,导致对承运人提出的索赔案件不断发生。文章拟从无单放货的表现形式入手 ,分析每一种形式中无单放货人的法律责任     

浅析湖南省第二次土地调查省级挂图编制方法

湖南省第二次土地调查工作先后历时3年,积累的数据成果能为相应专题地图编制提供数据基础。采用人工综合与计算机自动提取土地利用图斑,多种计算机自动综合相结合缩编基本农田要素,GIS空间分析方法进行耕地图斑坡度分级等关键技术,在多种专业制图软件相互配合下,将大比例尺的湖南省土地利用数据、基本农田数据、耕地坡度数据编绘成1∶80万湖南省土地利用现状图、湖南省基本农田分布图和湖南省耕地坡度分级图,能宏观、直观展示湖南省土地利用情况、基本农田分布情况和耕地坡度分级情况,更好为国土部门土地利用规划、基本农田保护与耕地潜力评价提供决策依据。     

应用AOP实现中间件的动态Reflection

结合AOP和元架构技术给出了一种对象动态自适应的解决方案，该方案基于分离关注原理并分两阶段。首先，确保系统需要动态自适应关注点被清晰的分离；然后使各自分离的关注点能够动态自适应。最后，给出了一个原型系统的初级框架。     

地理信息系统在物联网发展中的机遇

温家宝总理于2009年11月3日在人民大会堂向首都科技界发表了题为<让科技引领中国可持续发展>的讲话.温家宝总理指出:"使中华民族跻身于世界民族之林,必须依靠科学技术.历史告诉我们,科学技术决定民族兴衰和国家命运.信息网络产业是世界经济复苏的重要驱动力.全球互联网正在向下一代升级,传感网和物联网方兴未艾.     

THEORETICAL CALCULATION OF THE COEFFICIENT OF VISCOSITY OF WATER BY NONEQUILIBRIUM STATISTICAL MECHANICS OF SIMPLE LIQUID

The liquid state, especially water, has long been regarded as the most formidable problem in the field of statistical mechanics. The coefficient of viscosity of water is calculated using a simplified model based on the microstructure of water and methods similar to that used by Rice et al. (1961) who studied the non-equilibrium statistical mechanics of simple liquids, such as liquid argon. derived From the Liouville equation. This work's singlet and doublet equations are somewhat different From those derived by Rice et al. (1961) and solutions were obtained by the perturbation method. The calculated coefficient of viscosity of water at different temperature. using this method agree well with standard values.     

中国省级行政区边界形状的GIS分析

本文借助GIS软件支持,计算了我国32个省的平面轮廓形状的分维、形状指数和紧凑度。结果表明,形状复杂(边缘复杂程度高,破碎度较大)的省(区)主要集中在东部及沿海地区;形状较紧凑的省(区)主要集中在西部地区,东部省与西部省的形状特征有较大差异。另探讨了各地区的地理气候环境和历史人文特征所致的主要原因,并初步分析了各特征对交通等组织的影响。     

湖北省气象局网络安全系统设计

通过介绍湖北省气象局网络安全需求，分析了现有网络系统存在的安全弱点，利用目前主要的网络安全防御技术，设计了一个整体的网络安全系统，为湖北省气象局网络安全系统的后续建设和实施提供了具体的思路。     

ANALYSIS OF THE EXPANSION OF THE BUILT-UP AREA OF DALIAN CITY

With the astounding development and gradual improvement of remote sensing technology, as an advancedscience technology, it is used to provide multi-temporal, large scope real-time information for land-use dynamic change,and also is one of the best efficient methods for studying the earth resources and environment. Remote sensing image hasits characteristics of ample information and reflecting the objective realities. The paper uses multi-temporal TM images in1986, 1996 and 2000, and relevant statistic data to analyze land-use changes of Dalian City in Liaoning Province of Chi-na over ten years by means of the correlation analysis method. The results have shown that two methods could con-formably reflect the present land-use change. Urbanization is closely correlative to natural factors and economic develop-ment. Especially in recent 20 years, under the influence of the reform and open-up policy, Dalian, as a specific coastcity, is becoming an international metropolis.     

红鳍笛鲷弧菌病病原的研究

从广东湛江特呈岛鱼排取患病红鳍笛鲷进行病原分离、纯化,得到一株病原菌Ls 1。该菌 为革兰阴性短杆菌,有运动性,菌落半透明,最适温度28～30℃,需盐生长,氧化酶反应阳性,发酵 葡萄糖不产气,不发酵肌醇,对弧菌抑制剂O/129敏感。经API ID32E细菌鉴定系统及弧菌科细 菌生化鉴定管鉴定,该菌为一株溶藻弧菌(Vibrioaginolyticus);其对红鳍笛鲷的半数致死量为6.32 ×105mL-1。药敏试验结果表明,该菌对阿莫西林、先锋V不敏感,对新生霉素、卡那霉素、多粘菌 素B、四环素中度敏感,而对庆大霉素、氟哌酸、氯霉素、红霉素、链霉素和复方新诺明等有较高的敏 感性。     

断层相互作用的模拟研究

以库仑破裂应力变化计算为基础,研究断层的相互作用。从震源断层和投影断层两个角度出发,研究5种地震断层模型分别描述地震断层和投影断层时库仑破裂应力变化的分布特征,分析震源断层以及投影断层的不同滑动机制对库仑破裂应力变化分布的影响。分析发现,断层的相互作用与断层的滑动机制关系密切;走滑断层还与其左右旋有一定关系;除斜滑断层外,其他断层之间的相互作用呈现一定的对称关系。