半滑舌鳎精子的超微结构

硬骨鱼类精子的超微结构,国内外已有较为广泛的研究,一般均为鞭毛型精子,由头部、中片和尾部三部分组成.但不同种硬骨鱼的精子,其细胞核、中心粒复合体、袖套和鞭毛等的结构和形态都各有特异之处.     

莱州群体花鲈渔业生物学特征的研究

笔者对莱州群体花鲈的调查资料为基础,较系统地报告了该群体的形态异质性、群体组成、年龄生长、性成熟与繁殖力、饵料食性以及洄游分布等生物学特征,从而不仅为中日花鲈的物种鉴定、种群划分提供基础资料,并为该鱼的人工增养殖及资源保护提供渔业生物学依据.     

南方蓝鳍金枪鱼渔业和生物学的研究进展

南方蓝鳍金枪鱼(Thunnus maccoyii)是金枪鱼渔业的重要经济鱼种,历史上其全球年产量曾达到8万t水平[1]。由于长期的过度开发,近年来资源衰退严重,渔获量不断下降,各国已纷纷提出并实施一些养护措施[2~4]。目前中国正大力开发远洋金枪鱼渔业,而国内对南方蓝鳍金枪鱼的专项研究匮     

Seasonal variation of hepatic mixed function oxidases in winter flounder (Pseudopleuronectes americanus)

Indices of hepatic mixed function oxidase (MFO) activity in winter flounder (Pseudopleuronectes americanus) were measured at approximately monthly intervals from November 1983 to October 1985. Benzo[a]pyrene hydroxylase and ethoxyresorufin O-deethylase activities and cytochromes P450 and b₅ were generally, but not significantly, higher in males than in females. The MFO activity varied seasonally, reaching a maximum during or shortly after spawning. Variation in MFO enzyme activity between sexes was never greater than 2-fold at any time, and within sex, no greater than 6-fold during the seasonal cycle. This variability is less than that caused by exposure to environmentally realistic levels of some pollutants; measurements of MFO activity in this species might therefore be used to indicate environmental contamination.     

廉州湾红树蚬的繁殖生物学研究

2011年8月至2012年7月,采集广西北海廉州湾红树蚬(Polymesoda erosa)的周年样品进行了性腺发育、生殖周期和胚胎发育研究。结果表明,红树蚬雌雄异体,性别可通过成熟生殖腺颜色区分;1年1个生殖周期,性腺发育可分为增殖期、生长期、成熟期、排放期和休止期5个阶段,性腺发育两性同步;繁殖期从6月延续到11月,其中7月和10月为繁殖高峰期;肥满度指数最高为5.19%(5月份),最低为1.98%(2月份);繁殖方式为非孵育型,沉性卵,外被一层厚的胶质卵膜,水温26℃时,D形幼虫孵出时间约为27h。     

Research on the short-term fishery forecasting of Spanish mackerel (Scomberomerus niphonius)

-This paper presents the use of the hydrographic factors in short-term fishery forecasting of the spawning migration stock of the Spanish mackerel and salinity describes more concretely the correlativity of water temperature, salinity and air temperature with the fishing season in spring. The data have been collected from the hydrographic environmental investigation at the fixed position on the sea and the telegraph recordings of the drift net operation in the spring fishing season during the period of April and May from 1972 to 1980. The correlation coefficients of various factors with the data of the fishing season have been calculated by using the monadic regression method.The main reference targets of the forecasting are: (1) By using the upper-layer water temperature as the forecasting factor at the beginning of the fishing season, the accuracy is high; (2) the distribution and location of the isotherm of the upper-layer water at 10°C at the beginning of April are used as an important factor for d     

大西洋浪蛤繁殖生物学研究

于2002年1月至2003年1月 ,在浙江温州对引种养殖的大西洋浪蛤(Spisulasolidissima)的性腺发育、生殖周期、肥满度、胚胎发育、幼虫发育及变态等进行了研究。研究结果表明 ,大西洋浪蛤性腺发育过程分为增殖期、生长期、成熟期、排放期、休止期5个阶段。在浙南沿海的大西洋浪蛤繁殖期在4月下旬至6月上旬 (水温19.5～25℃ ) ,可大量产卵二次 ;肥满度最高出现在5月份,为10.37 % ,最低出现在2月份 ,为4.66 %。个体产卵量为14.1×104～111.1×104粒 ,卵沉性 ,卵径50～60μm ;受精卵在水温19.5～23℃ ,经16～20h孵化成D形幼虫 ;初孵D形幼虫平均大小为75μm×62μm ,浮游幼虫经12～13d培养进入壳顶后期 ,15～16d进入附着变态期 ,壳长为260～290μm ,发育变态为稚贝。     

A comparative study of the differential blood cell counts of winter flounder (Pseudopleuronectes americanus) collected in New England

Major alterations in the distribution of peripheral blood cells were observed among winter flounder (Pseudopleuronectes americanus) from Martha's Vineyard (MV), East Cape Cod Bay (ECCB), Fox Island (FI) (Narragansett Bay), Central Long Island Sound (CLIS), Gaspee Point (GP) (Narragansett Bay), Black Rock Harbor (BRH) (Bridgeport, CT), New Bedford Harbor (NBH) and Quincy Bay (QB) (Boston Harbor). Collection locations ranged from relatively uncontaminated offshore (MV) and nearshore (ECCB) sites to the more contaminated estuarine (FI, CLIS) and urban embayments (GP, BRH, NBH, QB). Differential diagnosis demonstrated a shift in lymphocyte counts from 28% (MV) to 54% (QB) that increased with the incidence of liver lesions in relation to known and suspected levels of sediment chemical contamination. A significant change in the ratio of circulating immature to mature erythrocytes was observed in flounder collected from Boston Harbor. Immature erythrocytes comprised 18% of the total erythrocyte population of fish from QB in comparison to 6% observed in flounder from ECCB.     

海州湾前三岛海域底播刺参群体特征初探

海州湾前三岛海域是中国刺参(Apostichopus japonicus)自然分布的最南界,2013年3月至2014年4月,采用潜水样带法,调查了前三岛海域底播刺参群体的体质量结构及季节变动特征,结合水下观察和视频调查,比较了不同生境类型中的刺参密度。结果表明,前三岛海域刺参密度为0.4~1.33头/m2,生物量为42.69~103 g/m2,刺参群体相对稳定,群体的体质量呈现冬春季节增长、夏秋季节下降的特点。不同生境类型中的刺参密度不同,大型藻类分布的礁石区刺参密度明显大于没有藻类分布的礁石区和砂质区,上述结果为揭示岛屿海域的刺参群体特征和资源养护提供了基础数据。     

Do bigger fish arrive and spawn at the spawning grounds before smaller fish: Cod (Gadus morhua) predation on beach spawning capelin (Mallotus villosus) from coastal Newfoundland

A relationship between body size and time of spawning has often been described for both pelagic and non-pelagic fish species that migrate for the purpose of spawning. The present study investigates this relationship for capelin (Mallotus villosus), a pelagic smelt-like species that spawns on the beaches of Newfoundland. Simple linear regressions were carried out separately for three groups of capelin: ovid females, spent females and males in three successive years (1982–1984). Bigger fish arrived near the spawning grounds first, for all three groups in all three years and was most obvious for female capelin. Analyses of stomach contents of Atlantic cod (Gadus morhua), an important predator of capelin in the Newfoundland area, showed a similar decrease in mean size of capelin throughout the capelin spawning season in June, July and August. Furthermore, analyses strongly suggest that early in the spawning seasons, when capelin abundance was high, cod selected for bigger capelin, whereas towards the end of the spawning seasons, when capelin abundance was low, cod did not show any size preference.     

海州湾鹰爪虾栖息地适宜性研究

根据2011年及2013−2017年春季和秋季在海州湾进行的底拖网调查数据,结合同步测定的底层水温、底层盐度、水深和资源量等数据,开展鹰爪虾(Trachypenaeus curvirostris)栖息地适宜性的研究,先利用广义加性模型对环境因子进行筛选,再应用提升回归树模型确定各环境因子的权重,然后分别采用算术平均法和几何平均法建立栖息地适宜性指数模型,并通过交叉验证选择最优模型。结果表明：春季鹰爪虾的栖息地适宜性指数模型采用算术平均法构建,选择水深和底层盐度作为变量,具有最小的拟合;秋季鹰爪虾的栖息地适宜性指数模型采用几何平均法构建,选择底层水温和底层盐度作为变量,具有最小的拟合。对春季栖息地适宜性指数模型总偏差贡献率最大的是水深(76.23%),其次是底层盐度(23.77%);对秋季栖息地适宜性指数模型总偏差贡献率最大的是底层水温(82.56%),其次是底层盐度(17.44%)。海州湾春季鹰爪虾的最适栖息水深为24 m以内,底层盐度为29.7～31.8;秋季的最适栖息底层水温为18～24℃,底层盐度为29.2～31.5。本研究表明,环境因子的优化有助于改进栖息地适宜性指数模型,并提升其预测能力。     

The effect of fishing the anguillid elver (Anguilla japonica) on the fishery of the Yangtze estuary

Based on the fishing net surveying data about anguillid elver of the Yangtze estuary collected in 2006 and historical data from previous research of the region, this study was focused on analyzing the fishing effort and catch variations of the elver, revealing the effects on fishery resources caused by elver fishing activities. It was found that the elver fishing activities took place in the tidal area of the Yangtze estuary from January to April. The yearly elver catch in 2000–2006 was 3 times greater than that in the 1970s–1980s. The catch per unit effort (CPUE) (ind./net/day) of the elver had some characteristics of temporal variation. It was higher in spring tide periods than that in neap tide periods, and the CPUE in February and March was 3–4 times greater than that in January. Twenty-eight fish species, 6 shrimp species, 2 crab species and 8 other invertebrates were found in the by-catch of the eel nets. Among the fishes, shrimps and crabs, the individual proportion of fishes is the highest, and they are mostly juveniles or small-size fishes, while the shrimps and crabs also make up a certain proportion. Most of the individuals in the by-catch were juveniles and were dead when they were harvested. It was proven that the activity of elver fishing has a great negative effect on regional fishery resources, though it has brought great commercial benefit. Relevant management and conversation should be reinforced.     

乐清湾水交换特征研究

采用EFDC模式模拟研究了乐清湾水交换的三维过程和时空变化特征,并通过计算水示踪剂质量浓度分析水体置换过程。结果表明,乐清湾水交换主要是由鹿西岛两侧流入的外海水体与湾内水体的交换,以及乐清湾口门西侧附近的湾内水体与瓯江北口径流冲淡水之间的交换。从口门到湾顶,水交换能力差别较大。以最窄的连屿至打水山断面为界,以南水体1个月基本可以完全交换,而以北水体2个月后仍然无法交换至湾口水平。连屿至打水山断面以北地形复杂,岛屿较多,污染物主要通过岛屿间的潮汐汊道输运,断面的瓶颈效应也使得断面以北的水体交换能力稍弱。在口门附近90%以上的水体被外海置换所需时间不到5 d,而此时湾顶水质未有太大改变;15 d左右,80%湾内水体被外海水置换;90%湾内水体被置换仅需40 d;70 d时的水体置换率达97%。     

Xenobiotic metabolizing enzyme activity in sockeye salmon (Oncorhynchus nerka) during spawning migration

Activities of the cytochrome P450 monooxygenase (MO) system and GSH-transferase (GST) were monitored about every 2 weeks in sockeye salmon during their sexual migration and ultimate death. Liver cytosol and microsomes were prepared from the tissue of 50 individuals, five males and five females at each collection. The activities observed were not significantly different between the sexes. Substantial changes occurred in cytochrome P450, cytochrome b₅ and the monitored activities during the migration. The most dramatic changes occurred at the spawning grounds and were evident even before spawning occurred. The cytochrome P450 and cytochrome b₅ contents increased while the MO and GST activities decreased. Pre-spawning MO and GST activities were similar to those reported for other species undergoing sexual maturation. Migrating salmon offer an interesting model for studying the regulation of sex-dependent and terminal forms of cytochromes P450, MO and phase II activities.     

象山港水交换特性研究

在验证良好的三维斜压潮流数学模型的基础上,以溶 解态的保守性物质为示踪剂,建立对流-扩散型的海湾水交换数值模型,计算了象山港水体半交换时间和平均滞留时间,并研究了斜压动力对湾内外水交换的贡献。研究结果表明,象山港水交换速度的区域性变化较大,水体半交换时间和平均滞留时间由象山港口门向湾顶逐渐增加,口门附近半交换时间在5d以内,平均滞留时间为5~10 d;湾顶水交换速度缓慢,水体半交换时间为30~35 d,平均滞留时间为35~40d。斜压动力对狭湾外段水交换影响较弱,对狭湾内段有较大的影响。     

Maternal transfer of bioactive polychlorinated aromatic hydrocarbons in spawning chinook salmon (Oncorhynchus tschawytscha)

The biological potency (relative to 2,3,7,8-tetrachlorodibenzo-p-dioxin. TCDD) of planar polychlorinated aromatic hydrocarbons (PCHs) in extracts of eggs and flesh from spawning female chinook salmon (Oncorhynchus tschawytscha) from Lake Michigan was determined by measuring the induction of 7-ethoxyresorufin O-deethylase activity in H-4-II-E rat hepatoma cells. TCDD-equivalents in flesh and egg samples ranged from 0 to 115·8 pg/g, and were approximately 5-fold greater in eggs than in flesh. These results suggest that the maternal transfer of PCHs may play a role in determining the reproductive success of Lake Michigan chinook salmon.     

Recruitment of shrimp (Pandalus borealis) in the Barents Sea related to spawning stock and environment

The shrimp spawn in autumn, and the females carry their eggs as out roe until spring when the larvae hatch. Within a period of 2 months the shrimp larvae settle to the bottom. It has been claimed that the year-class strength probably is determined during the larval phase. Today's assessment and forecast of the shrimp stock productivity and potential fishing yields are weak. This is partly due to poor knowledge on population dynamics from hatching until the shrimp are caught in the fishery at the age of 3 or 4 years. We, therefore, here identify the most important abiotic and biotic factors that affect recruitment in addition to spawning stock biomass. Since 1995, a net attached to the underbelly of the survey trawl used at the annual cruise in the Barents Sea has caught juvenile shrimp. The abundance of settled shrimp larvae varies in time and space. The recruitment to the fishery has been quite stable with the exception of the 1996 year-class, which was observed as 1-year-olds but has not been registered since. The temporal pattern of the three youngest year-classes is studied in relation to abiotic factors such as sea temperature, ice index and North Atlantic Oscillation, as well as biotic factors such as spawning stock biomass and presence of copepods, euphausiids and predating cod. Recruitment indices and factors identified by the Spearmann correlation to be significantly correlated with recruitment were used as input in a principal component analysis (PCA) and a generalized additive model (GAM) was applied. Abundance of 1-year-old shrimp is positively correlated to spawning stock biomass the previous year and to temperature of the previous winter, and negatively correlated with the number of 1-year-old cod. Two-year-old shrimp show significant correlation with temperature, whereas there is a strong negative correlation with euphausiids. Three-year-old shrimp are significantly correlated with the number of 2-year-old shrimp the previous year but negatively correlated to temperature at sampling time. This is probably due to less overlap with the main predator cod when cold. Ricker functions indicate an increased density-dependent mortality with age. When predicting the recruitment of shrimp to the fishery, the spawning stock biomass, the abundance of cod and euphausiids, as well as the temperature should be included.     

The biology of the catfish Cnidoglanis macrocephalus (Plotosidae) in an Australian estuary

This paper describes the age structure, growth, diet and aspects of gonadal development in the cobbler, Cnidoglanis macrocephalus (Valenciennes), in the large Swan estuary in south-western Australia between August 1982 and June 1984. Analysis of otolith annuli showed that while the 0+ to 3+ age classes were regularly represented in monthly samples, the 4+ and more particularly the 5+ and 6+ were much less abundant. The weighted means for the back calculated lengths at the end of the first to fourth years of life were 181 mm (≡ 26 g), 314 mm (≡ 156 g), 418 mm (≡ 410 g) and 518 mm (≡ 833 g) respectively. The mean length at the end of the second year of life was similar to the minimum legal size for capture by commercial fishermen (320 mm). The von Bertlanffy growth curve calculated from the back calculated lengths was L_t = 917 [1 − e^{−0·20(t + 0·11)}]. The relative weight of mulluscs, crustaceans and polychaetes in the intestine varied markedly between small and large fish, apparently reflecting differences in the size of these prey. The large mean diameter of mature eggs ( ) was correlated with a low mean absolute fecundity (2078). Trends shown by egg size, gonadosomatic index and time of appearance of spent females indicate that spawning takes place between October and December. The attainment of sexual maturity is both age- and size-dependent. Although sexually maturing and occasionally spent fish were found in the lower estuary, meristic values, commercial catch statistics and other data indicate that the cobbler found in the Swan estuary are part of a population which typically spawns at sea.