Recent researches of bioactive metabolites in marine organisms-associated microorganisms

1　Introduction　Sincetheearly 196 0s ,oceanhasbeenconsideredasanewanduntouchedsourceofpotentiallyusefulcom pounds .Theresultsfromtheresearchesontheorgan ismssuchassponges,softcorals ,algae ,ascidians ,bryozoansandmollusks ,havedemonstratedthatma rineorga…     

Mitigation of hydrogen permeation into steel by bacteria:a new research proposal

A new research proposal was introduced aiming at solving the fundamental theory for reducing the risk of hydrogen embrittlement(HE) in high-strength steels by utilizing hydrogen-consuming microorganisms.The superior performance of high-strength steel can meet the material strength requirements for remote deep-sea marine engineering development.Due to the heavy corrosive marine environment,steel structures must be protected by cathodic protection.However,high-strength steel is sensitive to stress corrosion cracking and HE,and cathodic protection can promote hydrogen permeation into steel.Hydrogen-consuming microorganisms are widespread in the natural environment and they utilize the energy of hydrogen oxidation to survive.If we could make use of the hydrogen-consuming function of microorganisms to consume the hydrogen generated during the cathodic protection process,then the potential for cathodic protection can be reasonably lowered,ideally protecting the steel and simultaneously reducing the possibility of HE.     

Effect of carbon and nitrogen ratio control on Artemia growth,water quality,biofloc microbial diversity under high salinity and zero-water exchange culture condition

Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species.The growth of heterotrophic microorganisms can be stimulated through adding extra carbon,which reduces the nitrogen level in the water and provides microbial protein to the animals.However,most of the studies and practical applications have been conducted in freshwater and marine environment.This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms.The effect of carbon supplementation on Artemia growth,water quality,and microbial diversity of bioflocs was studied in the closed culture condition without any water exchange.The salinity of the culture medium was 100.A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen(C/N)ratio of 5,15,and 30(Su5,Sul5,and Su30),respectively.The culture without adding sucrose was used as a control.Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level.The results showed that sucrose supplementation at higher C/N ratio(15 and 30)significantly improved the Artemia survival,growth and water quality(P0.05).Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume(BFV)(P0.05).The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness,and shaped the microbial composition at genera level.This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions.     

Exopolysaccharides from marine bacteria

Microbial polysaccharides represent a class of important products of growing interest for many sectors of industry. In recent years, there has been a growing interest in isolating new exopolysaccharides (EPSs)-producing bacteria from marine environments, particularly from various extreme marine environments. Many new marine microbial EPSs with novel chemical compositions, properties and structures have been found to have potential applications in fields such as adhesives, textiles, pharmaceuticals and medicine for anti-cancer, food additives, oil recovery and metal removal in mining and industrial waste treatments, etc. This paper gives a brief summary of the information about the EPSs produced by marine bacteria, including their chemical compositions, properties and structures, together with their potential applications in industry.     

Influences of Oyster Shell Soil Conditioner on Soil and Plant Rhizospheric Microorganisms

Oyster shell soil conditioner had significant influence on soil and rhizospheric microorganisms in their biomass, respiratory intensity and nutritional requirement. It could stimulate growth of soil and rhizospheric microorganisms, especially nitrogen-fixers, and intensify soil respiration in proportion to the dose and fertilizing time of the conditioner, leading to the increase in the number of nitrogen fixing bacteria and the decrease in the number of bacteria with special nutrition demands.     

Studies on culture condition and extracellular hydrolase of psychrophilic bacteria from Arctic sea ice

Arctic sea ice in the polar region provides a cold habitat for microbial community. Arctic sea ice microorganisms are revealed to be of considerable importance in basic research and potential in biotechnological application. This paper investigated the culture condition and extraceIlular hydrolase of 14 strains of different Arctic sea ice bacteria. The results showed that optimal growth temperature of strains is 15 ℃ or 20 ℃. The optimal pH is about 8.0. They hardly grow at acid condition. 3 % NaCl is necessary for better growth. These strains have different abilities in producing amylase, protease, eellulase and lipase. Pseudoalteronomas sp. Bsi429 and Pseudoalteronomas sp. Bsi539 produced both cellulose, protease and lipase. These results provide a basis for further developing and exploiting the cold adapted marine enzyme resources.     

EM技术在我国水环境保护中的应用研究进展

对EM技术在我国水环境保护中的应用情况进行了综述,重点介绍了EM技术应用于废水处理领域的研究进展,同时还展望了将来的应用及研究前景.     

氧化还原滴定的通用公式及其应用

本文导出了氧化还原滴定反应计算的通用公式,它适用于有H~+参加和涉及不对称电对的氧化还原反应。通用公式是非线性的,可用于计算滴定体系的氧化还原电位,绘制滴定曲线和计算滴定误差,文中还讨论了它的解法。     

Isolation and characteristics of one marine psychrotrophic cellulase-generating bacterium Ar/w/b/75°/10/5 from Chuckchi Sea,Arctic

Microorganisms living in polar zones play an important part as the potential source of organic activity materials with low temperature characteristics in the bio-technological applications. A psychrotrophic bacterium (strain Ar/w/b/75°/10/5) , producing cellulose at low temperatures during late-exponential and early-stationary phases of cell growth, was isolated from sea ice-covered surface water in Chuckchi Sea, Arctic. This bacterium, with rod cells, was Gram-negative, slightly halophilic. Colony growing on agar plate was in black. Optimum growth temperature was 15℃. No cell growth was observed at 351 or above. Optimum salt concentration for cell growth was between 2 and 3 % of sodium chloride in media. Maximal cellulase activity was detected at a temperature of 35℃ and pH8. Cellulase was irreversibly inactivated when incubated at 55℃ within 30 min. Enzyme can be kept stable at the temperature no higher than 25℃. Of special interest was that this bacterium produced various extracellular enzymes i     

Pharmacokinetics and Biodegradation Performance of a Hydroxypropyl Chitosan Derivative

Hydroxypropyl chitosan(HP-chitosan) has been shown to have promising applications in a wide range of areas due to its biocompatibility, biodegradability and various biological activities, especially in the biomedical and pharmaceutical fields. However, it is not yet known about its pharmacokinetics and biodegradation performance, which are crucial for its clinical applications. In order to lay a foundation for its further applications and exploitations, here we carried out fluorescence intensity and GPC analyses to determine the pharmacokinetics mode of fluorescein isothiocyanate-labeled HP-chitosan(FITC-HP-chitosan) and its biodegradability. The results showed that after intraperitoneal administration at a dose of 10 mg per rat, FITC-HP-chitosan could be absorbed rapidly and distributed to liver, kidney and spleen through blood. It was indicated that FITC-HP-chitosan could be utilized effectively, and 88.47% of the FITC-HP-chitosan could be excreted by urine within 11 days with a molecular weight less than 10 k Da. Moreover, our data indicated that there was an obvious degradation process occurred in liver( 10 k Da at 24 h). In summary, HP-chitosan has excellent bioavailability and biodegradability, suggesting the potential applications of hydroxypropyl-modified chitosan as materials in drug delivery, tissue engineering and biomedical area.     

Identification and characterization of microbial agents in a bloom of floating microorganisms in the Hailang River, China in 2006

In February 2006, a large amount of unknown floating microorganisms appeared in the Hailang River (HR) in the City of Hailin, Heilongjiang Province, China. The microorganisms caused the river water fouling and threatened the clean water supply of the city. To identify the unknown floating organisms, morphological and histological inspection, PCR, cloning and sequence analysis were conducted. The results revealed that the unknown floating organisms in the river were a novel species that likely belonged to oomycetes in Saprolegniales. The organisms were named Saprolegnia sp. HL0602.     

时空统计学在贫困研究中的应用及展望

消除贫困是人类社会的共同目标.贫困分布具有明显的空间特征,同时呈现出空间异质性和空间相关性.时空统计学以时空分析为优势,在贫困的时空分布及形成机制研究中发挥了重要作用.本文综述了不同时期我国贫困分布的空间特征、贫困数据的空间类型和特征以及贫困时空分布的影响因素,并总结了时空统计学方法在贫困空间研究中的4类应用,包括:探...     

Bromophenols from marine algae with potential anti-diabetic activities

Marine algae contain various bromophenols with a variety of biological activities,including antimicrobial,anticancer,and anti-diabetic effects.Here,we briefly review the recent progress in researches on the biomaterials from marine algae,emphasizing the relationship between the structure and the potential anti-diabetic applications.Bromophenols from marine algae display their hyperglycemic effects by inhibiting the activities of protein tyrosine phosphatase 1B,α-glucosidase,as well as other mechanisms.     

基于加权最小二乘谱迭积算法探测超导重力观测弱信号

提出采用加权最小二乘谱迭积算法探测超导重力观测数据隐藏的弱周期信号,该算法基于不等权的观测序列和序列的自相关性和互相关性,以高分辨率提取序列潜在的共同信号,削弱或抵消噪声信号。并以实例进行了分析和验证。     

基于序列二次规划方法的高密度电阻率反演

针对高密度电阻率成像法反演计算中存在的困难，探讨了基于序列二次规划（SQP）方法建立可行的电阻率反演算法的问题。在对三维点电源二维地电体电位场模型有限元法正演计算的基础上．建立了基于SQP方法的高密度电阻率成像法反演算法。根据勘测中测量电极间距的不同，提出按不同的电极间距分别建立优化模型进行参数优化。通过对模拟电阻率模型和实际观测资料的反演计算．表明该算法进行高密度电阻率反演是可行的，其具有对初始模型无特殊要求、收敛速度快的特点。这一反演算法可望在高密度电阻率成像的反演计算中得到应用。     

海洋土曲霉C23-3与不同类型海洋微生物共培养对其次生代谢产物的影响

【目的】研究海洋土曲霉C23-3与不同类型海洋微生物共培养对其次生代谢产物的影响,挖掘土曲霉菌株C23-3的次生代谢潜力。【方法】将副溶血弧菌、枯草芽孢杆菌和胶红酵母的活菌或灭活菌体分别以不同时间与土曲霉C23-3共培养;采用DPPH自由基清除法和Ellman法分别评价各提取物的抗氧化和乙酰胆碱酯酶抑制活性,采用滤纸片法比较各提取物的抗菌活性,采用HPLC指纹图谱分析各提取物化学成分的差异。【结果与结论】发现不同类型的微生物与土曲霉共培养均可激活一些原本沉默的活性次级代谢产物;菌株及共培养物的化学拮抗能力、HPLC指纹图谱中的特征代谢产物变化揭示在活菌培养体系中土曲霉占竞争优势;而且灭活菌体中可能存在某些信号物质,在12 d时对土曲霉群体自感应信号分子丁内酯-I的产生具有比活菌更强的刺激作用。研究结果为深入挖掘海洋真菌代谢潜力及抗老年痴呆等活性先导化合物的发现提供基础。     

GPS/VLBI/SLR/InSAR组合在地球动力学研究中的应用

详细探讨了InSAR技术应用于地球动力学研究的巨大潜力和优势；讨论了InSAR与GPS等其它空间技术组合的可能性。提出了GPS／VLBI／SLR／InSAR组合的新设想，并详细讨论了该方法在地球动力学、现代地壳运动、地球体积和形状变化研究中的应用。在具体组合应用中，针对地壳不同形变特征给出了3种不同的数学模型。     

Two dimensional tensor product B-spline wavelet scaling functions for the solution of two-dimensional unsteady diffusion equations

The fourth-order B spline wavelet scaling functions are used to solve the two-dimensional unsteady diffusion equation. The calculations from a case history indicate that the method provides high accuracy and the computational efficiency is enhanced due to the small matrix derived from this method.The respective features of 3-spline wavelet scaling functions, 4-spline wavelet scaling functions and quasi-wavelet used to solve the two-dimensional unsteady diffusion equation are compared. The proposed method has potential applications in many fields including marine science.     

Occurrence and diversity of marine yeasts in Antarctica environments

A total of 28 yeast strains were obtained from the sea sediment of Antarctica.According to the results of routine identi-fication and molecular characterization,the strains belonged to species of Yarrowia lipolytica,Debaryomyces hansenii,Rhodotorula slooffiae,Rhodotorula mucilaginosa,Sporidiobolus salmonicolor,Aureobasidium pullulans,Mrakia frigida and Guehomyces pullu-lans,respectively.The Antarctica yeasts have wide potential applications in biotechnology,for some of them can produce b-galactosidase and killer toxins.     

Characterization of halophilic C50 carotenoid-producing archaea isolated from solar saltworks in Bohai Bay,China

Halophilic archaea comprise the majority of microorganisms found in hypersaline environments. C50 carotenoids accumulated in archaea cells are considered potential biotechnological products and possess a number of biological functions. Ten red colordes were isolated from brine water in a saltem crystaltizer pond of the Hangu Saltworks, China. 16S rRNA gene sequence analysis showed that the colonies belonged to the extremely halophilic archaea genera Halobacterium and Halorubrum. Two representative strains, Halobacterium strain SP-2 and Halorubrum strain SP-4, were selected for further study on the phenotypic characteristics and effects of salinity and pH on accumulation and composition of pigments in their cells. The archaeal strains were isolated and grown in a culture medium prepared by dissolving yeast extract （10 g/L） and acid-hydrolyzed casein （7.5 g/L） into brine water obtained from a I.ocal salt pond. Their optimum salinity and pH for growth were 250 and 7, respectively, although pigment accumulation （OD490/ mL broth） was highest at pH 8. In addition, at 150-300 salinity, increasing salinity resulted in decreasing pigment accumulation. Analysis of the UV-Vis spectrum, TLC and HLPC chromatograms showed that C50 carotenoid bacterioruberin is the major pigment in both strains.