Mitochondrial phylogenomics reveal the origin and adaptive evolution of the deep-sea caridean shrimps(Decapoda:Caridea)

The deep-sea is considered as the most extensive ecosystem on the Earth.It is meaningful for elucidating the life origins by exploring the origin and adaptive genetic mechanisms of the large deepsea organisms.Caridean shrimps have colonized and successfully adapted to deep-sea environments.They provide an ideal model to analyze the origin and adaptive evolution of modern deep-sea fauna.Here,we conducted the phylogenetic analyses of mitocho ndrial genomes(mitogenomes) from carideans,including 11 newly sequences reported in this investigation to explore the habitat origins,divergence times,and adaptive evolution of deep-sea(seamounts and hydrothermal vents) caridean shrimps.The results showed that the species of deep-sea Caridea formed a monophyletic group.Phylogenetic analysis supported that the deepsea caridean shrimps may originated from shallow sea.The hydrothermal vents alvinocaridid shrimps and Lebbeus shinkaiae from Thoridae underwent a second range expansion from seamounts to vent ecosystems.Estimates of divergence time showed that the caridean shrimps invaded into deep-sea at 147.75 Ma.The divergence of most of the modern seamount and hydrothermal vent species are in the late Cretaceous/early Tertiary.This may associate with the geological events of the Western Pacific,the climate change,and the global deep-water anoxic/dysoxic events during this period.Twenty-two potentially important adaptive residues were detected in the deep-sea shrimp lineage,which were located in atp6,atp8,cox1,cox3,cytb,nad2,nad4 l,and nad5.This investigation adds our understanding of the evolutionary history of deep-sea caridean shrimps,and provides insights into the mitochondrial genetic basis of deep-sea adaptation in this group.     

晚始新世-渐新世东南印度洋沉积物源-汇过程及其古气候指示意义

晚始新世-渐新世期间南大洋及其周边地区的古气候响应研究有助于我们更好地认识地质历史上的重大气候转型机制和预测未来地球系统对于气候突变的响应,然而迄今为止,仍缺乏对该区域周边陆地的古气候响应研究。基于国际大洋发现计划(IODP)369航次U1516站位深海沉积物的年龄框架以及常量、微量和稀土元素组成,确定了该沉积物主要来源于澳大利亚西南部大陆,进而重建了构造时间尺度上物源区的化学风化历史。在此基础上,探讨了晚始新世-渐新世气候转折期南大洋周边大陆的古气候演化过程及其对于全球气候变化和区域古地理改变的响应。在始新世-渐新世转折期[34.1,33.6) Ma和[31.3,29.8) Ma期间,物源区的古气候主要受控于邻近区域古地理格局重大变化的影响,具体表现为气候条件趋于干冷和陆表化学风化强度降低的特征。在[33.6,31.3) Ma和[29.8,25.2] Ma期间,物源区的古气候则主要响应全球气候的变化,在前一阶段由干冷向湿热转变,而陆表化学风化强度相应增高;在后一阶段,气候保持在相对稳定的干冷状态,陆表化学风化强度也较弱。     

Thermal and saline tolerance of Antarctic krill Euphausia superba under controlled in-situ aquarium conditions

As a key species of the Southern Ocean ecosystem, the thermal and saline tolerances of Antarctic krill(Euphausia superba Dana) are relatively unknown because of the challenging environment and complicated situations needed for observation have inhibited in-situ experiments in the field. Hence,the thermal and saline tolerance of krill were examined under in-situ aquarium conditions with different controlled scenarios. According to the experiments, the critical lethal times of krill were 24 h, 2 h and 0.5 h under 9℃, 12℃, and 15℃, respectively, and the estimated 50% lethal times were about 17.1 h and 1.7 h under 12℃ and 15℃, respectively. Additionally, the critical lethal times(the estimated 50% lethal times) of krill were approximately 14 h and 0.5 h(about 22.9 h and 1.7 h) of salinity under 19.7 and 15.9, respectively.The observed critical and 50% lethal times of krill were 0.5 h and approximately 1.4 h, respectively,salinity under 55.2. The critical and 50% lethal temperatures of krill were 13℃ and approximately 14.2℃,respectively. Additionally, the critical and 50% lethal salinity was 19.6 and approximately 17.5 for the lower saline(below normal oceanic salinity [34.4]) environment and 50.3 and approximately 53.2 for the higher saline(above 34.4) environment, respectively. The upper thermal and saline preferences of krill can be considered 6℃ and 26.8 to 41.2, respectively. These results can provide potential scenarios for predicting the possible fate of this key species in the Southern Ocean.     

NGS-metabarcoding revealing novel foraminiferal diversity in the Western Pacific Magellan Seamount sediments

Seamount is a unique deep-sea ecosystem widely distributed in the world.Its biodiversity is vibrant due to its specific geographical and hydrological conditions.However,the diversity and features of foraminifera in such an environment have rarely been studied.We extracted environmental DNA(eDNA) in sedime nts and amplified the partial small subunit ribo somal DNA(SSU rDNA) of fo raminife ra to understand the foraminiferal diversity from four sites in Magellan Seamount(Western Pacific Ocean).Partial S SU rDNA sequencing was conducted and 912 979 foraminiferal reads were obtained and gathered into 266 operational taxonomic units(OTUs).In the available dataset,a high proportion of rare OTUs and low identity OTUs in each studied sample showed that the Magellan Seamount foraminiferal community might have a high genetic novelty.The relative abundance of foraminifera varied between replicates probably due to the existence of bias in amplification process and patchiness of the deep-sea floor.It showed that the Magellan Seamount has a relatively high benthic foraminiferal diversity characterized mainly by monothalamiids(76.37% of total reads) in association with rotaliids(19.03 % of total reads),including planktic foraminiferal sequences(38.58% of rotaliids;7.36% of total reads).The remaining reads were assigned to miliolids(0.83 % of total reads) and textulariids(0.66% of total reads),and 3.11% of total reads are unassigned to a specific family.The co mparative analysis with foraminiferal assemblage s from coastal and deep-sea environme nts indicated that seamounts could aggregate species from a nearby deep-sea.     

Laetmonice iocasica sp.nov.,a new polychaete species(Annelida:Aphroditidae) from seamounts in the tropical Western Pacific,with remarks on L.producta Grube,1877

Laetmonice Kinberg,1856 is a remarkable genus characterized by having harpoon notochaetae in the polychaete family Aphroditidae.We describe a new species of Laetmonice,Laetmonice iocasica sp.nov.,found from seamounts on the Caroline Ridge in the tropical Western Pacific.The new species is readily distinguished from congeners,particularly those distributed in the Indo-Pacific Ocean by possessing45 segments with 18 pairs of elytra,and the tuberculated harpoon notochaetae in the elytrigerous segments,which are replaced by tuberculated notochaetae without recurved fangs on segments 4 and 5.Laetmonice iocasica sp.nov.is closely related to L.producta Grube,1877,but differs in both morphology and the genetic distance of the mitochondrial cytochrome oxidase subunit I(COI) sequences.Laetmonice producta Grube,1877 contained five varieties reported in various marine areas,which have been raised to species level.However,the records of L.producta from the Sagami B ay and Suruga B ay in Japan and in the south-eastern Australia remain obscure and probably represent a different species.The data indicate that L.producta,which was originally described from Kerguelen Islands in the Southern Ocean and later commonly found on the Antarctic shelf,is probably distributed only at high latitudes of the Southern Hemisphere.     

Southern ocean SST variability and its relationship with ENSO on inter-decadal time scales

Empirical orthogonal function (EOF) analysis reveals a co-variability of Sea surface temperatures (SSTs) in the Southern Hemisphere (0°-60°S). In the South Indian and Atlantic Oceans, there is a subtropical dipole pattern slanted in the southwest- north-east direction. In the South Pacific Ocean, a meridional tripole structure emerges, whose middle pole co-varies with the dipoles in the South Indian and Atlantic Oceans and is used in this study to track subtropical Pacific variability. The South Indian and Atlantic Ocean dipoles and the subtropical Pacific variability are phase-locked in austral summer. On the inter-decadal time scales, the dipoles in the South Indian and Atlantic Oceans weaken in amplitude after 1979/1980. No such weakening is found in the subtropical South Pacific Ocean. Interestingly, despite the reduced amplitude, the correlation of the Indian Ocean and Atlantic dipoles with El Nio and Southern Oscillation (ENSO) are enhanced after 1979/1980. The same increase in correlation is found for subtropical South Pacific variability after 1979/1980. These inter-decadal modulations imply that the Southern Hemisphere participates in part of the climate shift in the late 1970s. The correlation between Southern Hemisphere SST and ENSO reduces after 2000.     

Retrieve sea surface salinity using principal component regression model based on SMOS satellite data

The sea surface salinity(SSS) is a key parameter in monitoring ocean states. Observing SSS can promote the understanding of global water cycle. This paper provides a new approach for retrieving sea surface salinity from Soil Moisture and Ocean Salinity(SMOS) satellite data. Based on the principal component regression(PCR) model, SSS can also be retrieved from the brightness temperature data of SMOS L2 measurements and Auxiliary data. 26 pair matchup data is used in model validation for the South China Sea(in the area of 4?–25?N, 105?–125?E). The RMSE value of PCR model retrieved SSS reaches 0.37 psu(practical salinity units) and the RMSE of SMOS SSS1 is 1.65 psu when compared with in-situ SSS. The corresponding Argo daily salinity data during April to June 2013 is also used in our validation with RMSE value 0.46 psu compared to 1.82 psu for daily averaged SMOS L2 products. This indicates that the PCR model is valid and may provide us with a good approach for retrieving SSS from SMOS satellite data.     

Bacterial productivity in the Prydz Bay and its adjacent waters,Antarctic

1　IntroductionBacteriaandtheiractivitiesplayanimportantroleintheelementalbiogeochemicalcyclesandenergytransformingintheocean (Zhenetal.1 997) .DortchandPackard(1 989) proposedthatfoodwebsintheeutrophicwatersaredominatedbythebiomassofprimaryproducerswhilefoodwebsintheoligotrophicwatersaredominatedbythebiomassofmicrobes.Heterotrophicbacteriahadbeenshowntoplayanimportantroleinthedecompositionoflarge ,rapidlysinkingorganicparticleswithinandbelowtheeuphot iczone ,andfurthertoaffecttheelementaldyn…     

Features and spatial distribution of circumpolar deep water in the southern Indian Ocean and the effects of Antarctic circum polar current

The data from the Southern Ocean observations of World Ocean Circulation Experiment(WOCE) are used for analysis and illustration of the features and spatial distributions of Circumpolar Deep Water(CDW) in the southern Indian Ocean.It is learnt from the comparison among the vertical distributions of temperature/salinity/oxygen along the 30°E,90°E and 145°E sections respectively that some different features of CDW and the fronts can be found at those longitudes,and those differences can be attributed to the zonal transoceanic flow and the merizonal movement in the Circumpolar Deep Water.In fact,the zonal transoceanic flow is the main dynamic factor for the water exchange between the Pacific Ocean and the Indian Ocean or between the Atlantic Ocean and the Indian Ocean,and for the effects on the spatial distributions of the physical properties in CDW.     

Fatty Acid Composition of Euphausia superba,Thysanoessa macrura and Euphausia crystallorophias Collected from Prydz Bay,Antarctica

The information of trophic relationship is important for studying the Southern Ocean ecosystems.In this study,three dominant krill species,Euphausia superba,Thysanoessa macrura and Euphausia crystallorophias,were collected from Prydz Bay,Antarctica,during austral summer of 2009/2010.The composition of fatty acids in these species was studied.E.superba and T.macrura showed a similar fatty acid composition which was dominated by C14:0,C16:0,EPA(eicosapentenoic acid) and DHA(decosahexenoic acid) while E.crystallorophias showed higher contents of C18:1(n-9),C18:1(n-7),DHA and EPA than the former two.Higher fatty acid ratios of C18:1(n-9)/18:1(n-7),PUFA(polyunsaturated fatty acid)/SFA(saturated fatty acid),and 18PUFA/16 PUFA indicated that E.crystallorophias should be classified as a typical omnivore with a higher trophic position compared with E.superba and T.macrura.     

Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

Trimethylamine N-oxide (TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from different genera harbor high hydrostatic pressure (HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from fish which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affiliated to 23 genera of Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at different depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera of Alteromonas, Halomonas, Marinobacter, Photobacterium, and Vibrio showed capacity of TMAO utilization, but none of the isolated Acinebacter, Bacillus, Brevundimonas, Muricauda, Novosphingobium, Rheinheimera, Sphingobium and Stenotrophomonas did, indicating the utilization of TMAO is a species-specific feature. Furthermore, we noticed that the ability of TMAO utilization varied among strains of the same species. TMAO has greater impact on the growth of deep-sea isolates of Vibrio neocaledonicus than shallow-water isolates. Taken together, the results describe for the first time the TMAO utilization in deep-sea bacterial strains, and expand our understanding of the physiological characteristic of marine bacteria.

     

Sea Salt Sodium Record in a Shallow Ice Core from East Antarctica as a Potential Proxy of the Antarctic Sea Ice Extent in Southern Indian Ocean

Antarctic sea ice has experienced an increasing trend in recent decades, especially in the Ross Sea and Indian Ocean sectors. Sea ice variability affects greatly the maritime airmass transport from high latitude to Antarctic continent. Here we present a new ice core record of sea salt sodium(ssNa+) concentration at annual-resolution in the Princess Elizabeth Land spanning from 1990 to 2016, showing that this marker could be used as a potential proxy for reconstructing the sea ice extent(SIE) in the Southern Indian Ocean(SIO) given their significant correlation(R =-0.6, P 0.01) over the past 27 years. The correlation and composite analyses results show that the ssNa~+ at the 202 km inland from Zhongshan Station and the SIE changes in SIO are closely related to the Indian Ocean Dipole(IOD) and Southern Annular Mode(SAM). The northward wind in central SIO occurs during positive IOD and the strengthened westerlies occurs during positive SAM, both of which favor increased sea ice in SIO and lead to the decreased ssNa~+ concentration at the coastal site.     

Retrieving multi-scale climatic variations from high dimensional time-series MODIS green vegetation cover in a tropical/subtropical mountainous island

【Title】
There are knowledge gaps in our understanding of vegetation responses to multi-scale climate-related variables in tropical/subtropical mountainous islands in the Asia-Pacific region. Therefore, this study investigated inter-annual vegetation dynamics and regular/irregular climate patterns in Taiwan. We applied principal component analysis （PCA） on 11 years （2001~2011） of high-dimensional monthly photosynthetically active vegetation cover （PV） derived from the Moderate Resolution Imaging Spectroradiometer （MODIS） and investigated the relationships between spatiotemporal patterns of the eigenvectors and loadings of each component through time and multi-scale climate-related variations. Results showed that the first five components contributed to 96.4% of the total variance. The first component （PC1, explaining 94.5% of variance） loadings, as expected, were significantly correlated with the temporal dynamics of the PV （r = 0.94）, which was mainly governed by regional climate. The temporal loadings of PC2 and PC3 （0.8% and 0.6% of variance, respectively） were significantly correlated with the temporal dynamics of the PV of forests （r = 0.72） and the farmlands （r = 0.80）, respectively. The low-order components （PC4 and PC5, 0.3% and 0.2% of variance, respectively） were closely related to the occurrence of drought （r = 0.49） and to irregular ENSO associated climate anomalies （r = -0.54）, respectively. Pronounced correlations were also observed between PC5 and the Southern Oscillation Index （SOI） with one to three months of time lags （r = -0.35 ~ -0.43, respectively）, revealing biophysical memory effects on the time-series pattern of the vegetation through ENSO-related rainfall patterns. Our findings reveal that the sensitivity of the ecosystems in this tropical/subtropical mountainous island may not only be regulated by regional climate and human activities but also be susceptible to large-scale climate anomalies which are crucial and comparable to previous large scale analyses. This study demonstrates that PCA can be an effective tool for analyzing seasonal and inter-annual variability of vegetation dynamics across this tropical/subtropical mountainous islandin the Pacific Ocean, which provides an opportunity to forecast the responses and feedbacks of terrestrial environments to future climate scenarios.     

Relationships between two Pseudoalteromonas strains isolated from the Canada Basin and the Southern Ocean using a polyphasic approach

The evolutionary relationships and taxonomic position of two marine planktonic bacterial strains BSw20211 and BSw10014, isolated from the Canada Basin and from the Southern Ocean, respectively, were determined using a polyphasic taxonomic approach. There was a close phylogenetic relationship between the two strains and most phenotypic properties were shared. Nonetheless, they were found to belong to different species of the genus Pseudoalteromonas on the basis of genotypic analyses. Findings were consistent with the suggestion that gyrB gene sequence comparison and DNA-DNA relatedness might better define phylogenetic relationships of bacteria at the species level. However, a cut-off value of 90% gyrB gene sequence similarity was not reliable for the differentiation of species within the genus Pseudoalteromonas.     

Plant studies in Himachal Pradesh,Western Himalaya: a systematic review

Owing to a predicted rise in temperature, increase in precipitation and the escalating anthropogenic stresses, the Himalayan ecosystems are the most threatened non-polar regions of the world. In the past, there have been racing extinctions and range shifts of several life forms in this region, particularly of the plant species. Therefore, consistent qualitative and quantitative records on botanical baseline data on a regular basis are indispensable. The current communication attempts to provide a detailed account on the current state of knowledge and research gaps in floristic studies of Himachal Pradesh, Western Himalaya. Based on an extensive review of more than 140 studies in the form of scientific peer reviewed literature on floristics, medicinal and aromatic plants(MAPs), rare, endangered, threatened and endemic species and ethno-botany, it is revealed that the number of floristic studies in Himachal Pradesh have surged in the past years but studies pertaining to the lower plants remain meagre. Unfortunately, the remote valleys including micro-watersheds lying in the coldarid regions are relatively less studied in terms of available growing stock and population status of MAPs. Depleting traditional ethno-botanical knowledge has been the major concern and, many threatened MAPs need to be accessed globally following IUCN standards. Thus, documentation of the conservation status of key MAPs, setting aside conservation areas around viable populations of threatened species, developing germplasm banks of the priority MAPs and monitoring the impacts of climate change has been suggested.     

Changes in Tintinnid Assemblages from Subantarctic Zone to Antarctic Zone Along Transect in Amundsen Sea (West Antarctica) in Early Austral Autumn

Tintinnid ciliates are important pelagic microplankton.Most studies previously conducted in the Amundsen Sea have covered a relatively small latitude range and provided minimal information about tintinnid species composition and distribution.The present study was conducted to investigate tintinnid assemblages from the Antarctic zone(AZ)northward through the polar front(PF)to the subantarctic zone(SAZ).A total of 17 tintinnid species belonging to seven genera were collected,and 16 were identified.Results show that nine of the species are endemic to the Southern Ocean and they mainly inhabit the AZ near Antarctic continent with an abundant proportion exceeding 60%of total tintinnid.According to the tintinnid abundance distribution,the species were divided into four groups:Group I includes Acanthostomella norvegica,Codonellopsis glacialis,C.pusilla and Cymatocylis antarctica and mainly occurs in the northern boundary of the PF;Group II includes Cymatocylis convallaria forma calyciformis,an unidentified species,and Amphorellopsis quinquealata and mainly inhabits the PF;Group III includes Salpingella costata,Cymatocylis vanhoeffeni,C.convallaria forma cristallina,C.convallaria forma drygalskii,C.convallaria,Codonellopsis gaussi,and Laackmanniella naviculaefera and mainly occurs in the AZ near the Antarctic continent;and Group IV,which comprises Salpingella sp.and inhabits all zones.The new species of tintinnid(belonging to Group II)primarily inhabit the AZ but also are distributed in the PF,and they have large lorica-oral-diameter(LOD).The distribution ranges of tintinnid assemblages from the AZ to PF were determined,in addition to the different assemblages mixed in the PF.The information provided in this study increases our understanding of tintinnid assemblages from the Antarctic continent in the Antarctic Circumpolar Current and Antarctic waters.     

草地退化杂类草入侵遥感监测方法研究进展

近年来,受全球气候变暖及日趋频繁的人类活动的共同影响,我国草地生态系统出现了不同程度的退化,成为草地生态学界的研究热点。本文较系统总结了草地退化、杂类草入侵遥感监测方法的应用进展,指出了常规草地退化遥感监测方法的不足,并展望了今后的发展方向。研究发现草地退化在群落尺度上往往表现为优质牧草种类减少,杂类草种类增加。在草地退化过程中,杂类草侵入,往往导致植被覆盖度增加,因而传统的基于植被覆盖度、生产力和产草量下降的草地退化遥感监测方法,无法反映草地退化在植被种群方面的变化特征,尤其是杂类草入侵的草地退化过程。而目前基于高光谱数据的草地退化杂类草入侵监测方法,结合地面光谱测定和对退化草地物种特征微弱光谱差异的定量分析,能有效识别群落中杂类草种类,并反演出占群落的面积比例、高度和盖度等,可为草地退化监测及其治理提供群落演替过程及其态势的重要指标,为草地退化监测提供新的遥感技术方法。     

Trends of urban air pollution in Zhengzhou City in 1996–2008

Urban air pollution is a commonly concerned environmental problem in the world.Identification of air quality trend using long-term monitoring data is helpful to understand the effectiveness of pollution control strategies.This study,using data from six monitoring stations in Zhengzhou City,analyzed the changing trend in concentrations of SO 2,NO x /NO 2 and TSP/PM 10 in 1996-2008,based on non-parametric Mann-Kendall test and Sen’s slope estimator,and evaluated the comprehensive air pollution level using Multi-Pollutant Index(MPI).It was found that the concentration of each pollutant exceeded obviously the World Health Organization(WHO) guideline value,but the changing trend varied:SO 2 and NO 2 were significantly increased mainly due to an increase in coal consumption and vehicle number,while NO x,TSP and PM 10 decreased.The air pollution was serious,and differed markedly among the three functional regions:it is the most severe in the Industrial and Residential Area(IRA),followed by the Transportation Hub and Business District(THBD),and then the High-tech,Cultural and Educational Area(HCEA).Different from NO 2 concentration that had a similar change trend/rate among the function regions,the change rate of PM 10 concentration differed spatially,decreased much more obviously in THBD than other two regions.For the whole city,the comprehensive air pollution level declined gradually,illustrating that the air quality in Zhengzhou was improved in the last decade.     

A study on the Antarctic circumpolar wave mode-A coexistence system of standing and traveling wave

The Antarctic circumpolar wave （ACW） has become a focus of the air-sea coupled Southern Ocean study since 1996, when it was discovered as an air-sea coupled interannual signal propagating eastward in the region of the Antarctic Circumpolar Current （ACC）. In order to analyze the mechanism of discontinuity along the latitudinal propagation, a new idea that ACW is a system with a traveling wave in the Southern Pacific and Atlantic Ocean and with a concurrent standing wave in the southern Indian Ocean is proposed in this paper. Based on the ideal wave principle, the average wave parameters of ACW is achieved using a non-linear approximation method, by which we find that the standing part and the traveling part possess similar radius frequency, proving their belonging to an integral system. We also give the latitudinal distribution of wave speed with which we could tell the reason for steady propagation during the same period. The spatial distribution of the propagation reveals complex process with variant spatial and temporal scales--The ENSO scale oscillation greatly impacts on the traveling process, while the result at the south of Australia indicates little connection between the Indian Ocean and the Pacific, which may be blocked by the vibration at the west of the Pacific. The advective effect of ACC on the propagation process should be examined clearly through dynamical method.     

Predicting the sinkage of a moving tracked mining vehicle using a new rheological formulation for soft deep-sea sediment

The sinkage of a moving tracked mining vehicle is greatly af fected by the combined compression-shear rheological properties of soft deep-sea sediments. For test purposes, the best sediment simulant is prepared based on soft deep-sea sediment from a C-C poly-metallic nodule mining area in the Pacific Ocean. Compressive creep tests and shear creep tests are combined to obtain compressive and shear rheological parameters to establish a combined compressive-shear rheological constitutive model and a compression-sinkage rheological constitutive model. The combined compression-shear rheological sinkage of the tracked mining vehicle at dif ferent speeds is calculated using the Recur Dyn software with a selfprogrammed subroutine to implement the combined compression-shear rheological constitutive model. The model results are compared with shear rheological sinkage and ordinary sinkage(without consideration of rheological properties). These results show that the combined compression-shear rheological constitutive model must be taken into account when calculating the sinkage of a tracked mining vehicle. The combined compression-shear rheological sinkage decrease with vehicle speed and is the largest among the three types of sinkage. The developed subroutine in the Recur Dyn software can be used to study the performance and structural optimization of moving tracked mining vehicles.