饲料中过量添加晶体蛋氨酸和蛋氨酸羟基类似物对大菱鲆生长、饲料利用和抗氧化反应的影响

以初始体质量为(5.49±0.01)g的大菱鲆幼鱼为实验对象,探讨饲料中过量添加晶体蛋氨酸或蛋氨酸羟基类似物对大菱鲆生长、饲料利用和抗氧化反应的影响.以白鱼粉和豆粕为主要蛋白源,饲料中同时添加0.25％ DL-蛋氨酸和0.3％蛋氨酸羟基类似物钙盐(MHA)作为对照,在此基础上分别添加0.5％ DL-蛋氨酸、1.25％ DL-蛋氨酸、0.59％MHA、1.49％ MHA、1.25％ DL-蛋氨酸+0.85％柠檬酸钙,配制出6种实验饲料,使饲料中蛋氨酸和蛋氨酸羟基类似物(HMTBA)含量分别是1.35％、1.83％、2.50％、1.45％、1.37％、2.51％和0.22％、0.22％、0.22％、0.63％、1.38％、0.29％.养殖试验在室内流水系统中进行,持续10周,每个处理设5个重复,每个重复放养大菱鲆幼鱼30尾.结果显示,过量添加DL-蛋氨酸和HMTBA对大菱鲆幼鱼生长无显著影响(P＞0.05).过量添加DL-蛋氨酸和HMTBA对大菱鲆体组分、肥满度(CF)、脏体比(VSI)和肝体比(HSI)均无显著影响(P＞0.05),但柠檬酸钙添加组(处理6)体蛋白含量与基础饲料组相比显著降低(P＜0.05).对大菱鲆肝脏过氧化氢酶( CAT)、超氧化物歧化酶(SOD)和肝脏硫代巴比妥酸反应产物量(TBARS)进行测定,各处理组间均无显著性差异(P＞0.05).结果显示,在该实验条件下,过量添加晶体蛋氨酸(1.31～1.73倍)和HMTBA(1.32～1.75倍)对大菱鲆幼鱼的生长、饲料利用和抗氧化反应没有显著影响.     

海岛旅游效率空间格局演变及影响因素研究 ——以中国12个海岛县(区)为例

旅游产业是海岛地区的重要产业之一，借助 DEA 模型测度 12 个海岛县 （区） 的旅游效率，采用组合方法对海岛旅游效率的空间特征演变进行刻画，并通过 EVIEWS 软件分析海岛旅游效率的影响因素。 （1） 海岛旅游综合效率总体不高，技术效率是海岛旅游效率发展的主要驱动力，规模效率水平较低，海岛旅游发展能力未得到全面发挥。 （2） 海岛县 （区） 之间旅游效率总体差异不大，相对差异明显。海岛旅游效率空间状态演变由不稳定趋向于稳定，可能存在“贫困陷阱”和“俱乐部趋同”现象。 （3） 经济发展水平、生态环境、科技信息对海岛旅游效率的发展起到积极的推动作用，产业结构、交通条件、城镇化是海岛旅游效率发展的约束性因素。     

An experimental model application of wavescreen: Dynamic pressure, water particle velocity, and wave measurements

This paper analyses the results of an application of a piled wavescreen. Experimental measurements were undertaken in the laboratory conditions for a given structural configuration under the attack of regular and irregular waves. Dynamic pressure distribution along and around the inclined piles was obtained employing pressure transducers. Using these data, in-line dynamic wave forces acting on piles were also determined. Water particle (orbital) velocities were measured at seaward and landward of the wavescreen using two acoustic Doppler velocimeters (ADV) simultaneously. Furthermore, wave data were collected using resistance type wave gauges at the seaward and landward of the structure. Based on those data, wave attenuation performance of the wavescreen was explored for two different depth values. Findings showed that piled wavescreen can provide effective shore protection as an environmentally friendly coastal structure.     

The effect of salinity on growth and weight loss of juvenile plaice (Pleuronectes platessa,L): An experimental test

Previous population estimates of the 0+ plaice (Pleuronectes platessa L.) in the Firth of Forth, east central Scotland, did not take account of the Forth estuary west of the Forth bridges. Previous work found plaice in the estuary grew as fast as, or faster than, the outer firth plaice. It was hypothesised that salinity may affect growth rates of early 0+ plaice. This hypothesis was tested in a laboratory experiment, by exposing juvenile plaice to three different, but naturally — experienced by the juveniles, salinities; 25, 30 and 35. Plaice fed a minimum ration did not grow in length. Mean weight decreased at all three salinities, however, the lowest weight loss was found at the lowest salinity (25) and the highest weight loss was found at the highest salinity (35). The minimum feeding ration was halted and plaice were then fed ad libitum. Consumption rates were not significantly different during the ad libitum feeding, while significant differences in mean weight change were found between the highest and lowest salinities.     

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

An annual cycle of recruitment,growth and production in a Malaysian population of the trochacean gastropod Umbonium vestiarium (L.)

From July 1981 to July 1982 Umbonium vestiarium (L.) on a north Penang sand shore numbered 573–11 077 m^?2 (mean 4126 or 53·1 g dry tissue) near MLWN and 2164–12 414 m^?2 (mean 6500 or 59·8 g dry tissue) further downshore. Heavy recruitment of young became evident in June and July 1982 and a closely corresponding cohort of young was present in July 1981. Progression of this cohort indicated that young settling in May–June grew to full size (11–13 mm diameter) by January–March the following year and that virtually all died during their second year, presumably having spawned in March–May. Recruitment of young was chiefly on the lower shore but adults came to be more abundant and predominant on the upper shore. There is some evidence of migration upshore during growth.Production is estimated at 105·4 g dry tissue m^?2 y^?1 (2118 kj) at the lower shore level and this is almost double the 58·8 g m^?2 y^?1 (1176·6 kj) at the higher level. These values represent almost the entire secondary production across much of the sand flats.Possible causes of such a marked annual cycle in the very weakly seasonal tropics of the Malacca Strait are considered and it is suggested that monsoonal changes in wind, wave action and salinity might be involved.     

The effect of salinity on growth and weight loss of juvenile plaice (Pleuronectes platessa, L): An experimental test

Previous population estimates of the 0+ plaice (Pleuronectes platessa L.) in the Firth of Forth, east central Scotland, did not take account of the Forth estuary west of the Forth bridges. Previous work found plaice in the estuary grew as fast as, or faster than, the outer firth plaice. It was hypothesised that salinity may affect growth rates of early 0+ plaice. This hypothesis was tested in a laboratory experiment, by exposing juvenile plaice to three different, but naturally — experienced by the juveniles, salinities; 25, 30 and 35. Plaice fed a minimum ration did not grow in length. Mean weight decreased at all three salinities, however, the lowest weight loss was found at the lowest salinity (25) and the highest weight loss was found at the highest salinity (35). The minimum feeding ration was halted and plaice were then fed ad libitum. Consumption rates were not significantly different during the ad libitum feeding, while significant differences in mean weight change were found between the highest and lowest salinities.     

An experimental study on the flow characteristics of a standing wave: application of FLDV measurements

The determination of the characteristics of a standing wave boundary layer, such as water particle velocities and shear stresses, is a vital issue in the prediction of sediment transport rate. Thus, an accurate measure of boundary layer characteristics is a key factor in the study of the wave boundary layer. In this study, a fiber-optic laser Doppler velocimeter (FLDV) is applied to directly measure the velocity profile in the standing wave boundary layer. As the experimental data presented in this paper, the antinode points of standing waves are found to move in the temporal domain. The previous second-order solution for a standing wave boundary layer is insufficient for the prediction of larger Ursell numbers.     

Flow-induced vibration analysis of elastic propellers in a cyclic inflow: An experimental and numerical study

In this paper, the flow-induced vibrations of marine propellers in cyclic inflows are investigated both experimentally and numerically. A Laser-Doppler velocimetry (LDV) system is used to measure the axial flow velocity distributions produced by the seven-cycle wake screen in the water tunnel. A customized underwater slip ring and a single axis accelerometer sealed by silicon sealant are employed to measure the acceleration responses of rotating propeller blade. Numerical simulations of pressure fluctuations on the blades are performed using large eddy simulation (LES), while the forced vibrations of the propeller blades are obtained by a combined finite element and boundary element method. Experimental and numerical results are presented for two model propellers with the same geometries and different flexible properties, which show that the propeller blade vibrates at a frequency which is seven times as large as the axial passing frequency (APF) in the seven-cycle inflow. Moreover, the propeller blades are observed to resonance when the 7 APF excitation frequency is equal to the fundamental frequency of the propellers. The results indicate that both the inflow feature and the modal characteristic of blades contribute to flow-induced vibrations of elastic propellers.     

国际海上救援效率比较研究 ——以东海特定水域为例

为研究东海海域海上救援效率和国际协作的协调性,以国际救援为视角,基于ArcGIS构建了海事救援效率评价模型。根据巡逻船舶密度数据确定了救援船舶的概率分布,得出了救援船舶与遇险船舶的可能距离,同时考虑了风浪影响下遇险船舶的漂移以及巡逻船舶失速的情况,分别计算了常风向和次常风向下中国、日本和韩国在东海特定水域的海上救援效率。结果表明:以各国现有的巡逻船舶配备对研究区内漂移的遇险船舶展开救援时,常风向下先后抵达的顺序为韩国、日本和中国,驰援时间分别为5.14、8.18和9.67 h;次常风向下抵达的顺序为韩国、日本和中国,驰援时间分别为5.45、8.25和10.08 h;为提高我国的救援效率,需要在东海海域部署9～10艘巡逻船舶开展常态化巡逻。本文研究可为我国有关部门科学制定和完善海上救援方案,调整海上救援力量配备,进一步加强国际间的协调与合作提供参考。     

Finding a balance between economic performance and capacity efficiency for sustainable fisheries: Case of the Da Nang gillnet fishery,Vietnam

Marine capture fisheries in Vietnam are small-scale, multi-species, multi-gear, and open access regime. Under numerous governmental promotion programs, Vietnamese fishing fleets have expanded at an uncontrollable rate. This research analyzes the economic performance and capacity efficiency of the Da Nang gillnet fishery. Data were obtained from 45 gillnetters in Da Nang using questionnaires, which were administered between April and July of 2011. The results showed that large-scale vessels (engine power≥90 hp) had better economic performance than small-scale vessels (engine power<90 hp) with average gross revenue flows of 1346.7 and 750.7 million VND, respectively. The large-scale vessels further actualized positive profits (15% gross revenue), while the small-scale vessels demonstrated negative results (−0.4% gross revenue). The main operating costs of fuel and labor accounted for 44% and 26%, respectively, of the total operating expenses. However, the results of the data envelopment analysis (DEA) showed that the Da Nang gillnet fishery did not operate at full capacity and that the unused capacity and the technical inefficiency rates were approximately 21.2% and 10.8%, respectively. The over-use of fuel, net sheets, and number of days at sea in the large-scale vessels were 7%, 10%, and 3%, respectively, while these ratios in the small-scale vessels were 7%, 3%, and 7%, respectively. Overcapacity may be separated into two parts, one part resulting from the optimal technical use of inputs and a second part resulting from enhancing the current policies. As such, feasible policies may include implementing the buyback of small and unprofitable vessels, and reviewing subsidy programs.     

Influence of hydrocarbon injection on the compaction by pressure-solution of a carbonate rock: An experimental study under triaxial stresses

The preservation of good petrophysical properties (high porosity/high permeability) at great depth in carbonate rocks may lead to the existence of a deeply buried reservoir (DBR), a target of interest for the oil industry. One of the key processes controlling diagenesis of the burial environment is Pressure Solution Creep (PSC), an efficient compaction process responsible for the evolution of porosity and permeability in many carbonate reservoirs. In this experimental study, we examine the effect of i) the presence of oil in the pore space and ii) its timing of injection on the PSC process and the petrophysical properties of a carbonate rock. The experiments were performed using a flow-through high-pressure cell, allowing the simulation of the pressure/stresses and temperature conditions of a DBR. Multi-disciplinary data (mechanical, chemical, petrographical and petrophysical) demonstrate that, without oil in the pore space, the main diagenetic process is the PSC, a process reducing by three the initial porosity but having no influence on intrinsic water permeability. An early injection of oil prior to water circulation causes the inhibition of PSC by the coating of the grains, leading to the preservation of porosity. Conversely, a late injection of oil does not preserve initial porosity. The dataset obtained from these experiments show the importance of the timing of oil charging in a reservoir in the preservation of initial porosity at great depth by the inhibition of PSC. However, the coating of grains by hydrocarbons may also inhibit further diagenetic processes leading to a creation of secondary porosity at depth.     

Temporal and bathymetric variation of epiphyte cover and leaf biomass in a southern Posidonia oceanica (L.) meadow: the case of Mahdia coast,Tunisia

The aim of this research was to study spatial and temporal variation in epiphyte cover and leaf biomass of Posidonia oceanica in Eastern Tunisia. Sampling was conducted at four stations on the Mahdia coast during October and December 2010, and April and August 2011, which correspond respectively to autumn, winter, spring and summer in this area. Posidonia oceanica shoots were collected at two depths (5 and 10 m). Cover of macroinvertebrates and macroalgae was estimated on adult leaves. The results showed that leaf and epiphyte biomasses vary significantly according to sampling date, with the highest values recorded in August. We found a high diversity of epiphytic assemblages on the leaves of P. oceanica with clear qualitative and quantitative dominance of Rhodophyceae compared to other groups, followed by Phaeophyceae. Most epiphyte species on the leaves of P. oceanica in Eastern Tunisia are the same as those in other parts of the Mediterranean Sea. No bathymetrical variation in the epiphytic community was found in our study area, which can be explained by the high levels of water clarity in Mahdia.