极大螺旋藻铁型超氧物歧化酶的纯化及性质

极大螺旋藻 Ｓｐｉｒｕｌｉｎａｍａｘｉｍａ藻体ＳＯＤ聚丙烯酰胺梯度凝胶电泳呈现４条同工 酶带, Ｈ_ ２Ｏ_２明显抑制该酶活性, ＫＣＮ对酶活性无影响,确认为 Ｆｅ－ＳＯＤ经硫酸铵分部盐析、离子交 换柱层析及凝胶过滤,纯化到电泳单斑点均－程度．纯化的Ｆｅ－ＳＯＤ分子量为３９．３ＫＤ,亚基分子 量为２０ＫＤ金属元素分析表明,每个亚基含０ ５５个Ｆｅ原子,该酶在紫外区最大吸收峰值为 ２７５．８nｍ、该酶氨基酸组成与蓝、绿藻和高等植物的Ｆｅ－ＳＯＤ相似,但它含有较高的丙氨酸,酸性氨 基酸和碱性氨基酸比值与低等植物及原核生物相近而明显高于高等植物．     

铅污染对钝顶螺旋藻生长及某些生理性状的影响

通过在Ｚａｒｒｏｕｋ培养基中加入不同浓度的Ｐｂ＾２＋,研究水环境污染对钝顶螺旋藻生长,藻蓝蛋白和光合作用,膜脂过氧化反应及以超氧化物歧化酶为代表的自由基清除系统的影响。发现当Ｐｂ＾２＋浓度高于２０ｍｇ．Ｌ＾－１时,钝顶螺旋藻生长受抑制,藻细胞随着浓度的和升高和时间的延长而黄化死亡。     

隔河岩水库诱发地震的环境条件

隔河岩水库蓄水后相继发生地震１０００余次,共中最大震级ＭＬ＝３．３。微震活动与水库蓄水过程密切相关,属水库诱发地震。库水渗透、地下水储积、岩溶和现代应力场等因素的研究表明,水库蓄水使库区水文地质环境发生变化,从而诱发了一系列微震。     

最佳地电台台址条件与环境条件的讨论

地电台观测地电阻率及其变化以研究地震活动规律，首要的问题是如何选择或评估其台址的优劣，选取最佳地电台台址。文中讨论了地电台台址的地质构造条件、测区地下介质结构条件、介质水文条件、地形地貌条件、电磁干扰等对地电台站观测效能的影响，为选建和评估地电台台址提供较全面、系统的依据。     

螺旋藻(Spirulina)对慢性肝损伤小鼠肝组织抗氧化能力的影响

用四氯化碳导致小鼠肝损伤,各组每日灌胃不同剂量螺旋藻,检测了饲养45d的小鼠血清的ALT活力、肝组织的SOD活力和MDA含量,分别建立ALT、SOD活力以及MDA含量随剂量(C)变化的函数,求出各项临界参数值,并对参数的生物学意义进行解释,以观察螺旋藻对肝损伤小鼠肝组织中SOD活力和MDA的影响.实验结果显示:螺旋藻能提高小鼠SOD的活力并降低其MDA含量,进一步验证了螺旋藻可提高肝损伤小鼠肝组织的抗氧化能力,分析结果还显示螺旋藻对肝组织抗氧化能力的提高既具有一定的量效累积性,也存在量效局限性.     

地震地表破裂发育特点及环境条件分析

通过已有震害实例分析，总结出了地震地表破裂的发育特点，在此基础上，结合震源破裂的深、浅部环境条件和形成力学机制、震源破裂和地震地表破裂的相互关系，讨论了地震地表破裂形成的环境条件。认为其所处的地震地质区特点是影响地震地表破裂发育的宏观环境条件，而场地第四系松散地层是影响地震地表破裂发育的微观环境条件。第四系地层对地震地表破裂的影响主要取决于第四系地层的土动力学性状、不均匀性和土层厚度等因素。     

海底淤泥层对海洋工程地震作用环境的影响问题

本提出了海底淤泥的流变性质对海洋工程的地震 作用环境的影响问题,并进行了有关的讨论。     

百草枯和H2O2预处理提高盐泽螺旋藻对铅的耐受性

用１０μｍｏｌ．Ｌ＾－１的百草枯（Ｐａｒａｑｕａｔ）和２ｍｍｏｌ．Ｌ＾－１的Ｈ２Ｏ２对盐泽螺旋藻（Ｓｐｉｒｕｌｉｎａ ｓｕｂｓａｌｓａ）作预处理,以诱导提高藻细胞内超氧物歧化酶（ＳＯＤ）,过氧化氢酶（ＣＡＴ）和过氧化物酶（ＰＯＤ）的活性,随后用４０ｍｇ．Ｌ＾－１的Ｐｂ＾２＋进行肋迫试验,实验结果表明,预处理组的藻细胞生长量和光合速率的下降幅度明显较未预处理组小;预处理组的ＳＯＤ、ＣＡＴ活性虽有衰减     

洱源台观测环境条件变化对形变观测数据的影响

2007—2019年洱源台洞体应变及辅助硐室温度的长期观测数据中存在明显的分段特征,通过多测项数据对比、日志追踪、现场调查等方法分析查找导致数据分段的近场环境事件。结果表明:近场环境的某些细微变化能够引起形变观测数据振幅、频率、组分、稳定性的变化,导致前后数据的不一致,从而影响观测数据的稳定性。因此,必须尽可能保持近场环境恒久不变以确保观测数据的长期稳定性,从而避免前后数据可比性降低影响前兆数据在中短期地震预测中的应用价值。     

根据实驗室測量决定大气积分吸收函数的一些問题

本文给出了在非重迭线近似情况下,用实验室测量的吸收资料去决定大气积分吸收函数的普温转换关系式,导出了Curtis-Godson近似的有效性条件,并以此解释了利用C-G近似计算积分吸收函数及其加热率产生誤差的原因。     

光照强度对铜绿微囊藻(Microcystis aeruginosa)和斜生栅藻(Scenedesmus obliqnus)生长及吸收特性的影响

在不同的光照强度下,测定了铜绿微囊藻、斜生栅藻在不同生长期的藻细胞密度、粒径、叶绿素a浓度、浮游植物吸收系数以及比吸收系数.单因素方差分析表明,在整个培养周期中,光照强度对铜绿微囊藻及斜生栅藻的藻细胞密度、叶绿素a浓度以及440、675 nm处吸收系数均有着显著的影响.两种藻在光照强度为50μmol/(m2.s)条件下,藻细胞密度、叶绿素a浓度及吸收系数值相对最大.相关性分析表明:藻类特征波段440、675 nm吸收系数与叶绿素a浓度、藻细胞密度在不同光照条件下都存在着显著的正相关性,其中叶绿素a浓度与藻类吸收系数存在着幂函数的关系,而线性关系能更好的说明藻细胞密度与吸收系数之间的关系.在不同光照强度及培养时期,藻类比吸收系数在一定的范围内波动,随光强增加比吸收系数呈上升趋势.铜绿微囊藻440、675 nm处比吸收系数与叶绿素a浓度呈显著的负相关关系,而斜生栅藻比吸收系数与叶绿素a浓度之间无显著相关,体现了不同藻类由于色素组成及比例差异其色素包裹效应也各不相同.对不同光照强度下的铜绿微囊藻及斜生栅藻进行400～700 nm波段积分,得到了两者在5、50及100μmol/(m2.s)不同种光强下的平均比吸收系数分别为0.0144、0.0134、0.0160,0.0086、0.0088、0.0105 m2/(mg Chl.a),铜绿微囊藻比吸收系数明显大于斜生栅藻.     

镍添加对水鳖(Hydrocharis dubia)叶片镍和营养元素含量以及氧化胁迫和抗氧化系统的影响

采用营养液水培的方法,研究不同镍(Ni)添加浓度(0、0.05、0.10、0.15、0.20 mmol/L)对水鳖(Hydrocharis dubia)叶片中Ni元素积累、营养元素(Ca、Mg、K、Fe和Mn)含量、氧化胁迫和抗氧化系统的影响.结果显示:(1)叶片中Ni含量以浓度依赖的方式增加;(2)叶片中营养元素含量(Ca、Mg、K、Fe、Mn)也受影响,其中Ca、Mg含量随Ni浓度增加而显著上升;K含量随Ni浓度增加有所升高,但不显著;Mn含量虽在较大Ni浓度时有所下降,但处理组始终高于对照组;而Fe含量则随Ni浓度增加而下降;(3)Ni胁迫诱导了明显的氧化胁迫,主要表现为:O·-2产生速率以及过氧化氢和丙二醛含量显著升高,可溶性蛋白以及光合色素含量明显下降;(4)随着Ni浓度的升高,过氧化物酶活性逐渐升高,过氧化氢酶活性呈下降趋势,超氧化物歧化酶活性在Ni添加浓度为0.05 mmol/L时出现短暂升高随后下降;维生素E和黄酮含量随Ni浓度增加呈缓慢上升趋势;还原型谷胱甘肽未发生明显变化,非蛋白巯基和植物络合素含量均表现为先升后降的趋势;总抗氧化能力随Ni浓度升高先增强后减弱.由此可见,Ni添加引起水鳖叶片中Ni元素的累积和营养元素含量变化,同时产生明显的氧化胁迫,打破了抗氧化系统的平衡.     

Effects of oil on mangrove seedlings grown under different environmental conditions

The effects of oil treatments (one-time addition of 120 ml per plant and weekly addition of 15 ml per plant) and environmental conditions at time of oiling (air conditioned laboratory/diffuse light vs hot, direct sunlight) on the survival and growth of Rhizophora mangle and Avicennia germinans seedlings were examined. No oiled A. germinans survived longer than a few weeks. Both one-time and weekly oiling depressed survival, stem growth, leaf production and maximum leaf size in R. mangle. Significant interaction terms between oil treatment and environmental conditions occurred later (50 and 59 weeks) for R. mangle stem growth and earlier (21 and 23 weeks) for leaf production. Relative to other treatment combinations, R. mangle mortality was greatest and growth lowest in the combination of one-time oiling under hot, bright outdoor conditions. Results indicate that synergistic interactions between oil effects and environmental conditions may contribute to the wide range of effects reported in the literature in studies of oil contamination of mangroves.     

Source tracing of noble metal elements in Lower Cambrian black rock series of Guizhou-Hunan Provinces, China

The Lower Cambrian black rock series of South China is abnormally rich in noble metal elements. According to the concentrations, the ratios, the relations, the distribution and partition patterns of noble metal elements, the authors think that the noble metals and other elements are neither directly from extraterrestrial materials, nor from the products of normal marine sedimentation. The abnormal enrichment of noble metal elements is closely related with hydrothermal fluid that flew out on the sea floor through deep cycling and reaction with Proterozoic ultramafic-mafic igneous rocks forming noble metal rich fluid. It is possible to form industrial multiple-elementore-deposits, especially hydrothermal type platinum-group-element-ore-deposits in the region with strong hydrothermal action.     

水分条件对藜蒿(Artemisia selengensis)富集湿地土壤重金属的影响

基于鄱阳湖南矶山湿地土壤及藜蒿Cd、As污染风险评价,利用盆栽实验研究水分条件对区域土壤理化性质、砷镉形态及其上生长的藜蒿(Artemisia selengensis)重金属富集能力的影响.结果表明:土壤Cd含量超标,存在生态风险,藜蒿茎中As含量达食品污染限量标准,存在食用风险;藜蒿对Cd、As的富集能力受土壤水分环境影响,水分增加会造成土壤pH与有机质含量上升,抑制藜蒿富集Cd的同时促进富集As.线性回归计算显示土壤有效态As含量与藜蒿As富集量呈显著正相关,可以用来评价藜蒿As富集情况,而土壤有效态Cd含量与藜蒿Cd富集量间无显著相关性.结合区域土壤Cd、As污染情况,适宜控制水分为缺水或旱湿交替条件,可以降低藜蒿等湿地植物中Cd的富集量;适宜控制水分为淹水条件,可以减少土壤Cd、As有效态含量.     

Effects of environmental regulations on heavy metal pollution decline in core sediments from Manila Bay

We investigated the high-resolution heavy metal pollution history of Manila Bay using heavy metal concentrations and Pb isotope ratios together with ²¹⁰Pb dating to find out the effects of environmental regulations after the 1990s. Our results suggested that the rate of decline in heavy metal pollution increased dramatically from the end of the 1990s due to stricter environmental regulations, Administrative Order No. 42, being enforced by the Philippines government. The presented data and methodology should form the basis for future monitoring, leading to pollution control, and to the generation of preventive measures at the pollution source for the maintenance of environmental quality in the coastal metropolitan city of Manila. Although this is the first report of a reduction in pollution in Asian developing country, our results suggest that we can expect to find similar signs of pollution decline in other parts of the world as well.     

First experiments on the maternal transfer of metals in the cuttlefish Sepia officinalis

The aim of this study was to provide a first insight on the incorporation of eight metals in the eggs of the cuttlefish Sepia officinalis via maternal transfer, using radiotracer techniques (^110mAg, ²⁴¹Am, ¹⁰⁹Cd, ⁶⁰Co, ¹³⁴Cs, ⁵⁴Mn, ⁷⁵Se and ⁶⁵Zn). The cuttlefish was fed daily with radiolabelled crabs for two weeks; it then started to spawn every three days. Among the eight tracers, only ^110mAg, ⁷⁵Se and ⁶⁵Zn were significantly transferred to the eggs. The radiotracer distribution among the egg compartments showed that ⁷⁵Se and ⁶⁵Zn were accumulated mainly in the vitellus whereas ^110mAg was found in similar proportion in the vitellus and the eggshell. During the embryonic development, ⁷⁵Se and ⁶⁵Zn contained in the vitellus were progressively transferred to the embryo, likely to supply its metabolic needs in these essential elements. Although it has no known biological functions, Ag contained in both vitellus and eggshell was also transferred to the embryo. Overall, our results showed that transfer of Ag, Se, and Zn does actually occur from a female cuttlefish to its eggs, at least during the last two weeks before spawning.     

茭草(Zizania latifolia)在不同人工湿地中的生长适应性

茭草是太湖流域河、湖水陆交错带及低洼沼泽中普遍生长的挺水植物,其生长条件在当地挺水植物中具有代表性.根据该流域河道水陆交错带的现状与挺水植物生长特性,针对性地设计了置入式生态滤床、生物氧化池、潜流-表面流复合人工湿地和水平潜流人工湿地4种不同生境条件,研究茭草的生长适应性,为建立与恢复退化的河道水陆交错带植被群落提供科学依据和技术支持.结果表明,茭草能够适应不同生长环境,在沸石基质和水培环境中均能正常生长;不同环境条件下其生长速度和繁茂程度存在差异.     

中等实验规模下不同营养环境对苦草(Vallisneria natans)生长的影响

本实验分别选用武汉东湖中营养和富营养湖区的湖水和底泥,并在水柱中添加氮或磷以设置高营养、中营养、中营养添加磷、中营养添加氮等四种营养环境.测定这四种营养条件下栽培苦草(Vallisneria natans)的生长和生化指标变化,探讨不同营养环境对苦草生长的影响机制.实验结果表明,苦草的生物量、叶数和新芽数等生长指标在中营养环境最高,中营养环境添加磷次之,中营养环境添加氮较低,在高营养环境最低;苦草可溶性糖和游离氨基酸含量在高营养环境中最高,在中营养环境、中营养环境添加磷和中营养环境添加氮等处理间没有明显差异.结果分析表明,高营养环境影响苦草的碳氮代谢水平并抑制苦草生长,这可能是由于苦草过量富集高营养环境中的氮素造成的;中营养环境中氮的升高会在一定程度上抑制苦草的生长,而磷的升高对苦草生长没有明显抑制作用.     

Investigation of heavy metal pollutants at various depths in the Gulf of Izmit

In this study, we report results concerning the accumulation of heavy metals in seawater from Izmit Bay. The bay was divided into the three parts: the eastern, the central and the western basins. The goal of this study was to determine levels of heavy metals at various depths in the bay between April 2008 and May 2010. Liquid–liquid extractions were performed on seawater samples. An atomic absorption spectrophotometer was used to measure levels of six metals: lead, cadmium, chromium, iron, manganese and zinc. We applied our results to evaluate the status of pollution in the Gulf of Izmit. Significant seasonal differences in metal concentrations and higher concentrations of many metals in water near the shore are evidence for uncontrolled release of pollutants in the water.