Influence of temperature on glutathione level and glutathione-related enzyme activities of Antarctic ice microalgae Chlamydomonas sp.ICE-L

GSH system plays a role in the control of the redox balance state,anti-oxidation and protecting life from injury of ROS(reactive oxygen species).In present paper,the possible GSH system of Chlamydomonas sp.ICE-L has been investigated by evaluating GSH and GSH-related enzymatic responses at different temperatures using spectrophotometer methods.The results showed that the GSH system is correlated positively to low temperature,and other factors but GR are correlated negatively to high temperature.So GSH and GSH-related enzymes play an important role in the adaptation of Antarctic ice microalgae to low temperature.     

Study on changes of plasmalemma permeability and some primary inorganic ions of Antarctic ice microalgae （Chlamydomonas sp. ICE-L） in the low-temperature stress

The changes of plasmalemma permeability and some primary inorganic ions of Antarctic ice microalgae （ Chlamydomonas sp. ICE-L） in the low-temperature stress were examined. The plasmalemma of 1CE-L could maintain the stability at the freezing condition of -6 ℃. That signifies that it could maintain the proper function of plasmalemma and stability of the intracellular environment during sea ice formation. The function of inorganic ions on low-temperature adaptation of ICE-L was investigated by using the X-ray microanalysis method. Low temperature （0 - -6 ℃ ） induces Ca^2 ＋ concentration increment of cytoplasm, but after 24 h the content decrease quickly to normal value. As a matter of fact, Ca^2 ＋ plays an important role as the second messenger in the low temperature adaptation of ICE-L. In addition, low temperature also influences on the other primary inorganic ions transfer and the cell maintains activity by keeping ratio balance among different ions. Above all, it is necessary for Antarctic ice microalgae to survive and breed by maintaining the stability of K^ ＋ content and the balance of Na^ ＋/Cl^ -.