<Emphasis Type="Italic">Pseudolella major</Emphasis> sp. nov. (Axonolaimidae,Nematoda) from the intertidal zone of the East China Sea

A new species of free-living nematode, Pseudolella major sp. nov. is described from the intertidal mudflats of Ximen Island in the East China Sea. Pseudolella major sp. nov. is characterized as follows: body length ~1 300 μm; short cephalic setae, 2–3 μm long; amphidial fovea loop-shaped with elongated ventral branch; ventral branch 50–60 μm long and extending past the base of buccal cavity; buccal cavity cylindrical with arching or spherical base; three heavy outwardly-curving odontia with blunt tips located at anterior of buccal cavity; pharynx short with posterior bulb; ventral gland cell elongate oval-shaped, located posterior to pharyngo-intestinal junction; excretory pore situated at the level of the middle of buccal cavity; tail conical to cylindrical; spicules arched, with double-cephalate proximal end; gubernaculum with a strong dorsal caudal apophysis.     

First record of red filament threadfin bream, Nemipterus marginatus (Valenciennes, 1830) (Perciformes, Nemipteridae), from Chinese waters

We collected five specimens of threadfin bream from Beihai, Guangxi, China in March 2010. These were subsequently identified as red filament threadfin bream Nemipterus marginatus (Valenciennes, 1830), being the first record of this species from Chinese waters. N. marginatus is distinguished by the following characteristics: lower border of eye lies above a line from tip of snout to upper base of pectoral fin; mouth oblique, maxillary extending to lower anterior border of pupil; teeth in jaws in several rows, pointed; upper jaw with 3 to 5 pairs of small recurved canines; suborbital with straight lower edge and rounded posterior edge; pectoral fins extending to between level of anus and origin of anal fin; pelvic fins reaching to the first or second anal rays; caudal fin forked, upper lobe tails into a short reddish filament; dorsal fin bluish with a yellow margin distally and a broad yellow median band which subdivides posteriorly into 3 small bands.     

Oncholaimus zhangi sp. nov. (Oncholaimidae,Nematoda) from the intertidal zone of the East China Sea

A new species of free-living marine nematode is described from intertidal sediment of the East China Sea.The new species belongs to the Oncholaimidae family.Oncholaimus zhangi sp.nov.is characterized by males having a large ventral swelling situated just posterior to the middle of the tail,no precloacal papilla,and a row of 6–7 ventral genital setae.The tails of both males and females are similar,being conico-cylindrical in shape.The new species is distinguished from other closely related species by the presence of a prominent ventral swelling at the tail of males and a similar tail shape in both sexes.About 110valid species in this genus have been recorded to date.     

A review of genus Nuchequula (Teleostei: Leiognathidae) with the description of a new record from Chinese waters

We collected leiognathid fishes from the Hainan Island and Beibu Gulf between November 2007 and December 2008. Ten of these specimens were identified as Nuchequula blochii (Valenciennes 1835), which is the first record of this species in China’s waters. These specimens are distinguished by the combination of these characteristics: breast fully scaled, cheek naked, anteroventral profile of the lower jaw almost straight, a conspicuous dark blotch distally on the anterior part of spinous portion of dorsal fin, a yellow blotch distally on posterior part of spinous portion of dorsal fin, and a dark blotch on nape. This paper reviews the genus Nuchequula and describes three species that occur in China’s waters.     

Two new free-living marine nematode species of the genus Anoplostoma (Anoplostomatidae) from the mangrove habitats of Xiamen Bay,East China Sea

Two new species of free-living marine nematode from mangrove habitats in Xiamen Bay are identified.Anoplostoma tumidum sp.nov.is characterized by relatively short outer labial setae(0.86–1.00 h.d.),long tail(c 7.2–8.9,c' 8.3–10.5),an instinct swollen distal portion of slender spicule(Sc 94–101 μm),and well developed copulatory bursae without bursal papillae.A.tumidum sp.nov.differs from all valid species of genus Anoplostoma in copulatory apparatus of males with a distinct swollen distal portion of spicule,and a relatively obvious constriction of head.A.paraviviparum sp.nov.is characterized by relatively long outer labial setae(1.11–1.22 h.d.),and tail(c 6.6–8.5,c' 8.6–10.2);elongated spicules with distinct knob-like proximal and pointed distal ends(Sc 46–69 μm);distinct strip-like gubernaculum(length with 11–15μm);well developed copulatory bursae with precloacal papillae and post-cloacal papillae;and a distinct constriction of head.A.paraviviparum sp.nov.is similar to A.viviparum Bastian,1865,but differs in the reproductive mode of female and the constriction of head.     

乌苏里拟鲿(Pseudobagrus ussuriensis)精子的超微结构

利用透射电子显微镜观察乌苏里拟鲿(Pseudobagrus ussuriensis)精子的超微结构。结果表明,乌苏里拟鲿精子由头部、中段和尾部组成。头部呈球形或椭圆球形,核前端无顶体,后端有植入窝。中段包括中心粒复合体和袖套。植入窝从细胞核的后端陷入核中。中心粒复合体位于植入窝内,包括近端中心粒和远端中心粒。袖套与细胞核后端相连,袖套中含有丰富的线粒体。尾部主要结构是轴丝,为典型的"9+2"结构,轴丝外两侧为细胞质膜形成的对称性鳍样结构。尾部内无囊泡及其他细胞器。     

A new species and new recorded species of Lumbrineridae Schmarda, 1861 (Annelida: Polychaeta) from China

Based on material deposited in the Marine Biological Museum of the Chinese Academy of Sciences(Qingdao),a new species,Lumbrineris sinensis sp.nov.,as well as Augeneria albidentata(Ehlers,1908),which is recorded for the first time from coastal water of China,are reported in the present paper.The specimens examined were collected during 1958 to 1960 from the Yellow Sea,East China Sea and South China Sea at depths of 4 to 182 m.     

Embryonic and larval development in barfin flounder Verasper moseri (Jordan and Gilbert)

Broodstock of Verasper moseri (Jordan and Gilbert) aged 3–4 years old were selected, and reinforced cultivation was conducted to promote maturation under controlled water temperature and photoperiod conditions. Fertilized eggs were obtained by artificial fertilization, and the development of embryos, larvae and juveniles was observed continuously. The results showed that the fertilized eggs of V. moseri were spherical, with transparent yolk and homogeneous bioplasm, and had no oil globule inside. The average diameter of the eggs was 1.77±0.02 mm. The eggs of V. moseri were buoyant in water with salinity above 35. The cleavage type was typical discoidal. Young pigment cells appeared when olfactory plates began to form. Hatching occurred at 187 h after fertilization at a water temperature of 8.5°C. The newly hatched larvae, floating on the water surface, were transparent with an average total length of 4.69±0.15 mm. During the cultivation period, when the water temperature was raised from 9 to 14.5°C, 4-day old larvae showed more melanophores on the body surface, making the larvae gray in color. The pectoral fins began to develop, which enabled the larvae to swim horizontally and in a lively manner. On days 7–8, the digestive duct formed. The yolk sac was small and black. The yolk sac was absorbed on day 11. Larvae took food actively, and body length and body height clearly increased. The rudiments of dorsal and anal fin pterygiophores were discernible and caudal fin ray elements formed on day 19. On day 24, the larval notochord flexed upwards, and the rays of unpaired fins began to differentiate. Pigment cells converged on the dorsal and anal fin rays, and the mastoid teeth on the mandible appeared. On day 29, the left eyes of juveniles began to move upwards. Depigmentation began in some juveniles and they became sandy brown in color on day 37. Most juveniles began to settle on the bottom of the tank. The left eyes of juveniles migrated completely to the right side on day 50, when the average body length attained 2.5±0.18 cm, and juveniles accomplished metamorphosis to young. The embryonic and larval characters of several flounder species are compared.     

Reevaluation of cortical developmental patterns in Euplotes (s. l.), including a morphogenetic redescription of E. charon (Protozoa, Ciliophora, Euplotida)

We documented the pattern of cell development in Euplotes charon. The ontogenesis of this species was similar to many of its congeners, except for the formation of the caudal cirri. In E. Charon, a caudal cirrus is formed posterior to each of the rightmost two or three dorsal kinety anlage in the proter, and the second rightmost dorsal kinety in the opisthe. In addition, two caudal cirri are formed posterior to the rightmost dorsal kinety in the opisthe. This pattern of development represents a completely new type. Based on our evaluation, and in comparison with previous studies, we also conclude that the pattern of cell development is variable among species in the Euplotes genera. The variation is particularly evident during the formation of frontoventral and caudal cirri. Based on the segmentation pattern of frontal-midventral transverse cirral anlagen, cirri reduction, and migration of frontoventral cirri, we identified five types: the affinis-type, the eurystomus-type, the charon-type, the raikovi-type and orientalis-type. Euplotes (s. l.) can also be divided into three types based on the formation of caudal cirri: focardii-type, vannus-type and charon-type. Indeed, we conclude that the number (one or two) of marginal cirri should be given as much consideration as the genetic separation. Given this, we reassessed the validity of using genetic separation to classify the group. Generally, the morphogenetic data disagreed with the molecular data (SSrRNA gene sequences). Given these discrepancies, it is too early to draw conclusions on the systematic arrangement of this species-rich taxon.     

Daptonema parabreviseta sp. nov. (Xyalidae,Nematoda) from the Jiaozhou Bay of the Yellow Sea,China

A new species of free-living marine nematode of the genus Daptonema Cobb, 1920 is described from the sublittoral sediment of the Jiaozhou Bay, Qingdao. Daptonema parabreviseta sp. nov. is characterized by having narrowed anterior end; small funnel-shaped buccal cavity; short cephalic setae; spicules with bulbiform proximal end and pointed distal end; gubercunalum tubular shape with a blunt dorsal apophysis. The female genital system presents a spacious postvulval sac. The new species closely resembles Daptonema brevisetosum Thanh & Gagarin, 2009. However, it differs from Daptonema brevisetosum by having relatively narrowed head (10 μm vs 20 μm); spicules with bulbiform proximal end and pointed distal end, not bifurcated at distal end. Female genital system with a spacious postvulval sac (vs absent).

     

The ultrastructure of the spermatozoon of Octopus ocellatus Gray, 1849 (Cephalopoda: Octopoda)

Morphology of the spermatozoon of Octopus ocellatus was studied by light, scanning electron, and transmission electron microscopes. Sperm are 600–700 μm long, with a large number of granules in diameter about 130 nm. Each spermatozoon is composed of a head, neck, and tail. The head is made up of an acrosomal complex anterior to the nucleus. The spiral acrosomal complex consists of an electron-lucent vesicle, lacuna, and an electron-dense acrosomal vesicle. Additionally, the spiral acrosomal vesicle has numerous equidistant striations, and is surrounded by many small granules (20 nm diameter). A long straight nucleus, which is electron-densed, has a deep posterior concavity, the nuclear vacuole. At the terminal end of the nucleus is a sleeve-like structure with a concave posterior nuclear fossa (PNF). The neck is short connecting the PNF. The basal body is located in the PNF and gives rise to the axoneme. This structure connects the head, neck, and tail. The tail is divided into a middle piece and a principal piece. The middle piece, having a 9+9+2 arrangement, is surrounded by a mitochondrial sheath and terminates by an electron-dense fibrous sheath. The principal piece is the longest part of the sperm with coarse fibers tapering posteriorly. The results of this study shall provide some useful information for artificial breeding of this species.     

A new species of Nassarius (Gastropoda: Nassariidae) from the western Pacific Ocean

This paper describes a new species of Nassarius from the South China Sea,which was recognized when re-sorting the collection of Nassariidae in the Marine Biological Museum,Chinese Academy of Sciences,Qingdao,China.The shells were collected during several investigations,including the National Comprehensive Oceanic Survey in 1958-1959,and the China-Vietnam Co-Investigation on Marine Resource of the Beibu Gulf during 1959-1962.The morphology of the shell and the radula places the new species of Nassarius within the subgenus Zeuxis.It is named Nassarius(Zeuxis) nanhaiensis sp.nov.     

Use of calcein and alizarin red S for immersion marking of black rockfish Sebastes schlegelii juveniles

Black rockfi sh Sebastes schlegelii juveniles(30–40 mm total length) were immersed in a range of calcein(CAL) solutions at concentrations ranging from 50 to 250 mg/L and alizarin red S(ARS) solutions at concentrations ranging from 100 to 500 mg/L in fi ltered seawater(salinity 30) for 24 h. Fluorescent marks were detected in otoliths(sagittae, asteriscus), scales, fi n rays(dorsal, pectoral, ventral, anal, and caudal fi n rays), and fi n spines(dorsal, ventral, and anal fi n spines) after a 60-d growth experiment. With the exception of 50–100 mg/L CAL, acceptable marks were produced in the otoliths and fi n spines by all concentrations of CAL and ARS. In particular, marks were clearly visible under normal light in the sagittae, asteriscus, and fi n spines of fi sh immersed in 200–500 mg/L, 300–500 mg/L, and 200–500 mg/L ARS, respectively. Scales and fi n rays had acceptable marks at much higher concentrations(≥50 mg/L CAL, ≥300 mg/L ARS for scales and ≥50 mg/L CAL, ≥200 mg/L ARS for fi n rays). The mark quality was highest(i.e., acceptable marks were observed in all sampled structures after immersion marking) in fi sh immersed in 150–250 mg/L CAL or 300–500 mg/L ARS. In addition, there was no signifi cant difference in survival and growth of marked fi sh compared with controls 60 d post-marking( P 0.05).     

Taxonomic description of a new marine ciliate,Euplotes qingdaoensis n. sp. (Ciliophora: Euplotida)

The characteristics and infraciliature of a new euplotid ciliate,Euplotes qingdaoensis n.sp.,discovered from the coastal waters of Jiaozhou Bay,northern China,were studied using live observation and silver impregnation.The new marine species is characterized by a slightly fl attened body(90–110 μm×70–80 μm,in vivo),an adoral zone of 26–29 membranelles,10 strong frontoventral and fi ve transverse cirri,two caudal cirri,a single left marginal cirrus,seven dorsal kineties,a middle kinety with about 15 pairs of basal bodies,a silverline system of the double-eurystomus type,and a C-shaped macronucleus.     

中国鲉形目棘鲬属一新纪录种——丝鳍棘鲬

【目的】记述中国南海海域棘鲬属的一新纪录种,以厘定中国南海海域棘鲬属的分类。【方法】2019年4月于中国南海北部海域海南岛东南渔场采集棘鲬属样品,采用形态学鉴定方法研究其形态特征,描述其生态习性,编制中国棘鲬属分种检索表。【结果与结论】样品中有6尾为中国新纪录种——丝鳍棘鲬Hoplichthys filamentosus Matsubara&Ochiai,1950。主要特征:体延长,平扁,向后渐狭小,背缘低平,腹缘平直;背鳍VI,14~15;臀鳍17;胸鳍13+ⅲ;腹鳍Ⅰ-5;尾鳍17;侧线骨板27;鳃耙2+11~12;体具几条不明显深色横带;胸鳍具黑色斑点;第1背鳍分布黄色斑点,具黑色小点和黑边;第2背鳍中部分布黄色圆斑,每1鳍条根部具黄色圆斑;腹鳍白色,无斑纹;臀鳍无明显斑纹;尾鳍后缘具黑边,中部较随机分布黄色圆斑,下叶颜色较深。该种雌雄异形:1)雄性下颌骨腹外侧具棘,雌性则隐没皮下;2)雄性第2背鳍鳍条均显著延长呈丝状,雌性则无显著延长。     

Morphology and SSU rDNA sequence analysis of two hypotrichous ciliates (Protozoa,Ciliophora, Hypotrichia) including the new species <Emphasis Type="Italic">Metaurostylopsis parastruederkypkeae</Emphasis> n. sp.

The morphology and phylogeny of two hypotrichous ciliates, Metaurostylopsis parastruederkypkeae n. sp. and Neourostylopsis flavicana (Wang et al., 2011) Chen et al., 2013 were investigated based on morphology, infraciliature and the small subunit (SSU) ribosomal RNA gene (rRNA) sequence. The new species, M. parastruederkypkeae n. sp. was identified according to its characteristics: body shape ellipsoidal, size about (165–200) × (45–60) μm in vivo, cell color reddish; two types of cortical granules including wheat grain-like and yellow-greenish larger ones along the marginal cirri rows and dorsal kineties and dot-like and reddish smaller ones, grouped around marginal cirri on ventral side and arranged in short lines on dorsal side; 26–41 adoral membranelles; three frontal and one parabuccal, five to seven frontoterminal, one buccal, and three to six transverse cirri; seven to thirteen midventral pairs; five to nine unpaired ventral cirri, five to seven left and three to five right marginal rows; and three complete dorsal kineties. Phylogenetic analysis based on SSU rDNA sequences showed that both Metaurostylopsis and Neourostylopsis are monophyletic. As the internal relationship between and within both genera are not clear, further studies on the species in these two genera are necessary. The key characteristics of all known twelve Metaurostylopsis-Apourostylopsis-Neourostylopsis species complex were updated.     

Use of tetracycline hydrochloride and alizarin complexone for immersion marking black rockfish Sebastes schlegelii

We tested the utility of chemical marking techniques in the juvenile black rockfish Sebastes schlegelii. Juveniles(30–40 mm total length) were immersed in a range of tetracycline hydrochloride(TC) solutions at concentrations ranging from 300 to 500 mg/L, and alizarin complexone(ALC) solutions at concentrations ranging from 200 to 400 mg/L in filtered sea water(salinity of 30) for 24 h, respectively. Otoliths(sagittae, asteriscus), scales, fin rays(dorsal, pectoral, ventral, anal, and caudal fin rays), and fin spines(dorsal, ventral, and anal fin spines) were sampled and used to detect fl uorescent marks after a 60-day growth experiment. With the exception of 300 mg/L TC, acceptable marks were produced in the otoliths and fin spines by all concentrations of TC and ALC. In particular, we observed clearly visible marks in the sagittae, asteriscus, and fin spines under normal light at concentrations of 200–400 mg/L, 250–400 mg/L, and 250–400 mg/L ALC, respectively. Scales and fin rays had acceptable marks at much higher concentrations(≥350 mg/L TC, ≥250 mg/L ALC for scales and ≥350 mg/L TC, ≥300 mg/L ALC for fin rays). The best mark quality(i.e., acceptable marks were observed in all sampled structures after immersion marking) were obtained following immersion in TC at between 350–500 mg/L, and ALC between 300–400 mg/L. In addition, there was no significant difference in survival and growth of TC and ALC marked fish compared to their controls up to 60 days post-marking( P 0.05).     

Taxonomic Studies on a New Marine Ciliate, Apocoleps magnus gen. nov., spec. nov. （Ciliophora, Colepidae）, Isolated from Qingdao, China

The morphology and infraciliature of a new marine colepid ciliate, Apocoleps magnus gen. nov., spec. nov., are described based on living observations and silver impregnations. The new genus Apocoleps is characterized by having 8 (vs. 6 in most other related genera) armour tiers, spines at both ends of the cell, 3 adoral organelles and plates with 4 reniform uni-windows. Apocoleps magnus spec. nov. is defined by the following features: body elongated and slightly curved, about 100–120μm× 35–45 μm in vivo; an...