光合色素标记法测定不同类群浮游植物的光合速率和生长速率

尝试利用放射性同位素14C示踪方法测定浮游植物不同类群的光合速率和生长速率,并通过实验室和现场海域的培养实验来初步评估该技术的可行性.通过高效液相色谱法分离光合色素,手动收集并测定各对应光合色素峰的放射性活度,依据Redalje-Laws法并结合类胡萝卜素标记法计算出各大类群浮游植物的光合速率和生长速率.实验结果表明,在实验室纯种培养的4株藻光合速率范围为347.73~678.98 mg/（dm3.d）,最高的为绿藻的小球藻,最低的为定鞭金藻的球石藻.其生长速率范围为0.12~0.24 d-1,最高的为球石藻,最低的为小球藻.在现场海域各类群的光合速率相对较低,变化范围为0.63~68.78 mg/（dm3.d）,生长速率在0.07~1.68 d-1之间.本研究首次在我国海域通过现场原位测定得到不同类群浮游植物的光合速率和生长速率.但在测定过程中存在的样品含量和放射活度较低等问题还有待于进一步解决.     

Modeling photosynthesis and the growth of marine phytoplankton

The paper analyzes the most popular models of photosynthesis and growth of marine phytoplankton in the literature and demonstrates their limitations. A new approach to modeling is proposed and used to obtain new models of marine phytoplankton photosynthesis and growth. An important feature of the proposed models is their ability to describe coupled multisubstrate cyclical interactions typical of biochemical and physiological processes. As a first approximation, the mathematical models are represented by equations of nonrectangular hyperbolas. The models describe the stoichiometry of extraction of elements from the medium, whatever the degree of their limitation, an important feature in describing biogeochemical cycles of elements. This stoichiometry is governed by measurable internal parameters of an organism (substrate parameters) and can be a key cause of stoichiometric formation of elements in the ambient medium, described, for example, by the Redfield ratio. The substrate constants are fundamental characteristics of the models, which form “automatically” in the construction of model equations in arbitrary units.     

Comparison of the effects of short-term UVB radiation exposure on phytoplankton photosynthesis in the temperate Changjiang and subtropical Zhujiang estuaries of China

We examined the effect of solar ultraviolet radiation B (UVB, 280–315 nm) on photosynthesis of natural phytoplankton assemblages in the temperate Changjiang River Estuary (CRE) in the East China Sea (ECS), and the subtropical Zhujiang River Estuary (ZRE) in the South China Sea (SCS) from August 2002 to April 2003. The short-term effect of UVB was assessed by exposing samples in quartz tubes/bottles to solar radiation under three treatments: (1) natural sunlight (NS) with UVB (NS-UVB); (2) photosynthetically active radiation (PAR, NS cut off UVB); and (3) NS with additional artificial UVB (NS + A-UVB). Solar UVB apparently inhibited phytoplankton photosynthesis rates. In the temperate CRE-ECS, solar UVB reduced surface phytoplankton photosynthesis by about 28% in August and February, while in the subtropical ZRE-SCS the inhibition was only 22% in September and October. In the CRE-ECS, phytoplankton in the stratified water column displayed stronger UVB inhibition when deeper water samples were exposed to surface UVB. Phytoplankton in the mixed water column did not show strong UVB inhibition, while light shift exposure of deeper phytoplankton in the same water column to surface light produced similar results, indicating that mixing moderates UVB effects. In the ZRE-SCS, surface phytoplankton showed greater photoinhibition in January (sunny). However, in April (cloudy), phytoplankton showed little UVB inhibition. Incubation for a short time without UVB showed a large increase in Chl a at two stations in the ZRE-SCS, but a large decrease at the other station in the presence of UVB. In contrast, in the CRE-ECS, a similar incubation experiment without UVB showed a decrease in Chl a, and small UVB inhibition of Chl a at two stations. Nutrient conditions might have played a role in the difference of UVB inhibition between the two regions as the ZRESCS had relatively high concentrations of all nutrients while PO₄ was only 0.21 μM at one of the CRE-ECS stations. The results suggest that phytoplankton in temperate waters would be more responsive to variation of UVB than ones in subtropical waters.     

The thermal effects of cooling system of a thermal power plant on photosynthesis of marine phytoplankton

The activity of photosynthesis and the contents of chlorophylla and pheo-pigments were compared in the samples of the intake and effluent seawater of Owase-Mita Thermal Power Plant and in the seawater samples in the Yoshimi Bay before and after heat treatment. At Owase-Mita Thermal Power Plant, after passage of sea water through the cooling system chlorophylla content reduced to about a half and pheo-pigment content doubled in August with high temperature (25–27C), but the contents of chlorophylla and pheo-pigments changed little in January with low temperature (16C); whereas the depression of photosynthetic activity after passing through the cooling system was 71–77 % in August and 31–46 % in January. In the laboratory experiments, the heat treatment at 34–43C for 7–8 sec., which resulted in temperature rise of 7–8C, gave the decrease of chlorophylla and an increase of pheo-pigments in August temperature (26.5C), but a slight decrease of chlorophylla and a slight increase of pheo-pigments in November at the initial temperature of 19.2C, while in January and December the samples with low initial temperature (11.6 and 10.8C) showed almost no change in the content of pigments; the decrease in photosynthetic activity was 32 % in August sample,ca. 11 % in November sample,ca. 15% in January and almost no change in December. It is concluded that the photosynthetic carbon assimilation of marine phytoplankton is suppressed by passing through the cooling system of a thermal power plant even at lower water temperature in winter, while the content of chlorophylla is affected very little at a temperature below 20C.     

Selective transmission of light in the ocean waters and its relation to phytoplankton photosynthesis

Occurrence of the depth differences in pigment composition and photosynthetic properties of marine phytoplankton were examined in relation to the spectral changes of light with depth. Phytoplankton were taken from various depths in the northwestern North Pacific, and their absorption spectra were determined with intact cells and in 90% acetone extract. The photosynthetic activities of phytoplankton were concurrently measured under blue, green, red and white light. The difference in absorption spectra for the surface and deeper samples was considerably small, indicating that the prevailing green or blue light in the deeper layers may have little significance for depth-variations of the pigment composition in marine phytoplankton. The depth differentiation in the shape of the light-photosynthesis curve was marked in a well stratified water column but no active response of deeper phytoplankton to green light could be confirmed. The photosynthetic efficiencies of phytoplankton for blue and green light were approximately 105–115 % and 80–90 % of white light, respectively, irrespective of sampling depth.Contribution No. 261 from Shimoda Marine Biological Station     

Effects of some representative petroleum refinery effluent compounds on photosynthesis and growth of natural marine phytoplankton assemblages: Part 1—cresols

The effects of cresols on the photosynthesis and growth of natural marine phytoplankton assemblages from the Scripps Pier at La Jolla, California, USA were measured. In two experiments, in which dinoflagellates were the dominant algae, photosynthesis (measured by ¹⁴C uptake) was inhibited at concentrations > 100 μg litre^?1 and the concentration giving half-maximal photosynthesis was 10,000 μg litre^?1. In a third experiment, in which diatoms were dominant, photosynthesis was not inhibited until levels > 10,000 μg litre^?1 were reached and the half-maximal concentration was 20,000 μg litre^?1. These experiments suggested that dinoflagellates were more sensitive to cresols than diatoms. However, the reverse was true in an experiment using cultures. In a long-term growth experiment (natural assemblages from the Scripps Pier), only a level of 88,000 μg litre^?1 completely inhibited growth. There was a two-day lag in growth at 8500 μg litre^?1, but the algae recovered and grew as well as the controls which contained no cresols. This experiment was started with a mixed diatom-dinoflagellate-microflagellate population but only the diatoms grew. High concentrations of cresols (> 900 μg litre^?1) resulted in a decrease in the diatom Skeletonema costatum, compared with the controls. Otherwise there was no selection for resistant species. In these experiments, cresols were measured by extraction with methylene chloride from seawater followed by measurement by UV absorption. In all experiments cresol levels decreased with time. This was attributed to uptake by phytoplankton and bacterial degradation.     

Comet assay in phytoplankton as biomarker of genotoxic effects of environmental pollution

The alkaline comet assay was tested on different microalgae: the dinoflagellates, Karenia mikimotoi and Alexandrium minutum, and the diatom, Chaetoceros gracilis. The microalgae were exposed during their exponential growth to the model direct genotoxicant, hydrogen peroxide (1h, 5 and 100muM H2O2). Following H2O2 exposure, the comet assay was validated only for K. mikimotoi for which genotoxicity was observed from the lowest tested concentration of 5 microM with a concentration-dependent effect. C. gracilis was too small in size (4 microm) to be correctly analysed. For A. minutum, our lysis buffer was not strong enough to digest the cellulosic thecal plates. For K. mikimotoi, the comet assay was thus applied for the study of the genotoxic effects of different pesticides: epoxiconazole (as Opus formulation), chlorpyriphos-ethyl (as Dursban formulation) and endosulfan at 1, 10 and 100 microg of active substance/L for 24h. Exposure to epoxiconazole in formulation resulted in an increase in the extent of DNA strand breaks at the highest tested concentration icro/L. Endosulfan exposure resulted in DNA damage for K. mikimotoi nuclei. Genotoxicity was observed from 1 microg/L of endosulfan and was not concentration dependent.     

基于浮游植物吸收光谱提取粒径参数

在南海北部、大亚湾及珠江口3个不同水体生物-光学数据的基础上, 研究了浮游植物粒径结构的变化特征, 建立了基于浮游植物吸收光谱提取的浮游植物粒径参数(S<f>)的混合光谱模型。南海海区不同的水体环境下浮游植物的粒级结构有着很大的差异: 在河口和沿岸水体小型浮游植物占优势, 在外海水体微微型浮游植物占优势。浮游植物粒径参数随小型浮游植物增多而减少, 随微微型浮游植物增多而增大。叶绿素a浓度从外海到沿岸逐渐增大, 浮游植物粒径参数随叶绿素a浓度的增大而减小, 它们之间呈幂函数关系。结果表明, 利用混合光谱模型得到的浮游植物粒径参数与南海海区不同水体的生物-光学特征(粒级结构Rpico和Rmicro、粒级指数SI、叶绿素a浓度)有一定的相关性。具体的相关性表示为: S<f>与粒级结构(Rpico和Rmicro)存在一定的关系, 与小型浮游植物和微微型浮游植物之间的线性相关系数分别是0.55和0.65; S<f>与浮游植物粒级指数(SI)有较好的线性关系, 相关系数是0.57; S<f>与叶绿素a浓度呈幂函数关系, 相关系数是0.64。这个混合光谱模型为从光学参数反演浮游植物种群的生态学信息提供了有效的手段, 同时又可用于分析浮游植物优势粒径结构对光学特性的影响。     

Array technology as a tool to monitor exposure of fish to xenoestrogens

A variety of anthropogenic chemicals are capable of binding to the estrogen receptor of vertebrate species. Binding of these compounds can interfere with homeostasis by disrupting normal gene expression patterns. The purpose of this study was to investigate the feasibility of applying array technology as a monitoring tool for detecting the presence and distribution of estrogenic compounds in coastal habitats using sheepshead minnows as our model. cDNA clones that were isolated from differential display, including vitellogenin alpha and beta, vitelline envelope protein (ZP2), and transferrin, among others, were spotted on the macroarray. The results of these experiments demonstrate a characteristic expression pattern of estrogen responsive genes in sheepshead minnows exposed to 17 beta-estradiol (E2).     

Ammonium input to the sea via large sewage outfalls-Part 2: Effects of ammonium on growth and photosynthesis of southern California phytoplankton cultures

Cultures of six marine phytoplankton were grown at ammonium concentrations ranging up to 200 μg-atom NH₄---N litre⁻¹. Only the growth of dinoflagellates, Gymnodinium splendens and Gonyaulax polyedra was inhibited at the two highest concentrations used. In 3-h photosynthetic ¹⁴CO₂ uptake experiments, only Gymnodinium was inhibited at concentrations of NH₄---N greater than 100 μg-atom litre⁻¹. We conclude that the increased ammonium concentrations found near Southern California sewage outfalls would not be inhibiting to phytoplankton in the vicinity of such outfalls.     

Temporal and spatial changes of macroalgae and phytoplankton in a Mediterranean coastal area: the Venice lagoon as a case study

Since the late 1980s the lagoon of Venice, a shallow Mediterranean coastal area, has experienced strong environmental changes. Macroalgae, which were the predominant primary producers of the lagoon, reduced markedly, but neither phytoplankton nor seagrasses replaced them. Temporal and spatial changes in macroalgal standing crop (SC) and phytoplankton concentration were investigated between 1987 and 1998. Maps of macroalgal SC show a marked declining trend. Biomass in fresh weight decreased from: 558 ktonnes in 1987, to 85 ktonnes in 1993 and to 8.7 ktonnes in 1998. As a whole, the biomass in 1998 was only 1.6% of the biomass recorded in 1987. Similarly the macroalgal net (NPP) and gross (GPP) primary production decreased from ca. 1502 and 9721 ktonnes year(-1) to ca. 44 and 229 ktonnes year(-1), respectively. In the early 1990s the clam Tapes philippinarum Adams & Reeve and seagrasses, especially Zostera marina Linnaeus, colonised the bottoms free of macroalgae, but the development of intense clam-fishing activities prevented both phytoplankton blooms and seagrass spreading. Maps of chlorophyll a drawn according to data collected in parallel to macroalgal standing crop show unchanged concentrations. Macroalgae changes are enhanced by comparing annual trends in four areas of the central lagoon during 1989-1992 and 1998-1999. In those areas phytoplankton also decreased significantly. Marked changes of some environmental variables strongly associated with the primary production were recorded both during the lagoon mapping and in the areas studied on a yearly basis.     

Vertical flux of phytoplankton and particulate matter in the White Sea according to the long-term exposure of sediment traps

The vertical fluxes of phytoplankton (VF_Phyto) and particulate organic carbon (VF_POC) in the White Sea were determined using seven long-term (292 to 296 days) sediment traps moored at five stations at 67–255 m depths. The annual VF_Phyto and VF_POC ranged from 0.55 to 24.64 g C/m² and from 3.7 to 93.9 g C/m², respectively. The highest VFPhyto was observed in the Basin region located close to the Gorlo along the Tersk coast. The algal biomass accounted for 15–43% of the VF_POC. Diatoms comprised the most important group, accounting for 83–100% in the sedimented biomass. Thalassiosira nordenskioeldii dominated in the VF_Phyto at all the stations except for the station in the basin close to Onega Bay, where Ditylum brightwellii was the most abundant.     

Criterion for stability of phytoplankton patchiness using a Liapunov method

A criterion for the stability of plankton patchiness is developed using a Liapunov function. Plankton growth, advection and diffusion in a closed area are considered. A critical length scale for the stability does not exist but a more common criterion which contains the growth rateα, diffusion coefficientA _l and arbitrary function of growthf can be introduced in an integral form.     

Pressure transfer function in time and time-frequency domains

In this paper, we define a time-domain pressure transfer function calculated from SIWEH (smoothed instantaneous wave energy history) transforms, and a time-frequency domain pressure transfer function calculated from wavelet transforms, of synchronized wave and pressure data. It is our objective to study whether the time-domain pressure transfer function and the time-frequency domain pressure transfer function can provide new interpretation of wind wave behaviors. The detail structure of local time-frequency pressure transfer function in three-dimensional plot from wavelet transform is not employed due to its large variations, instead the time-integral wavelet spectral pressure transfer function and frequency-integral wavelet SIWEH pressure transfer function are used. These two averaged pressure transfer functions are smooth approximations of frequency-domain Fourier and time-domain SIWEH pressure transfer functions, respectively.Application to real ocean waves reveals that in frequency-domain the measured Fourier and wavelet spectral pressure transfer functions can be approximated by the linear pressure transfer function in the dominant wave range. In time-domain, the wavelet SIWEH pressure transfer function is a better indicator of wind wave behaviors than the SIWEH pressure transfer function. A value higher than 0.5 for the wavelet SIWEH pressure transfer function is a good discriminator of relative shallow-water long waves and wave groups are mostly composed of relative low frequency long waves.     

Removal of trace metals from seawater during a phytoplankton bloom as studied with sediment traps in Funka Bay,Japan

To study biological effects on the particulate removal of chemical elements from seawater, sediment trap experiments were carried out successively ten times throughout the spring phytoplankton bloom in Funka Bay. Sediment traps were deployed every one to two weeks at 1, 40 and 80 m depths. The settling particles obtained were analyzed for trace metals, phosphate and silicate. The propagation of diatoms in spring results in larger particulate fluxes than that of dinoflagellates. The biogenic silicate concentration is higher in the earlier period, when diatoms are predominant, than in the subsequent period, when dinoflagellates are predominant. The concentrations of aluminum, iron, manganese and cobalt in the settling particles comprising largely biogenic particles are lower during phytoplankton bloom. The concentration of copper is not reduced by the addition of biogenic particles, and its vertical flux is approximately proportional to the total flux, indicating that its concentration in the biogenic particles is nearly equal to that in the non-biogenic particles. The results for nickel and lead show the same tendency as for copper. Cadmium is more concentrated in biogenic particles than in non-biogenic particles, and the concentration of cadmium in the settling particles decreases with depth, similarly to phosphate and organic matter. Thus, metals in seawater are segregated by biological affinities, and the degree of incorporation into biogenic particles is in the order Cd > Pb, Ni, Cu > Co > Mn, Fe, Al. Biogenic particles are the most important agent controlling the vertical distribution of metals in the ocean. They remove the metals from the surface water, transport them through the water column, and regenerate them in the deep.     

1-Hydroxypyrene as a biomarker of PAH exposure in the marine polychaete Nereis diversicolor

The possibility of using the pyrene metabolite 1-hydroxypyrene as a biomarker of polycyclic aromatic hydrocarbons (PAHs) exposure was investigated by exposure of the marine polychaete Nereis diversicolor to several PAHs in the laboratory. Animals were exposed to pyrene alone and to five different PAHs – phenanthrene, anthracene, pyrene, benzo[a]pyrene and benzo[k]flouranthene. After five days of exposure the concentrations of parent PAHs and 1-hydroxypyrene were identified using three different analytical methods, high-performance liquid chromatography with fluorescence detection (HPLC/F), synchronous fluorescence spectroscopy (SFS) and gas chromatography with mass spectrometric detection (GC/MS). The SFS measurements of 1-hydroxypyrene were validated by the more sensitive method HPLC/F. The positive correlation between total PAHs and 1-hydroxypyrene concentrations in the polychaete tissues observed in experiments, suggests the feasibility of 1-hydroxypyrene as a suitable biomarker for total PAH exposure assessment. Furthermore, the possibility of employment of the simple and rapid SFS method instead of HPLC/F for biomarker analysis has been confirmed by the positive and significant correlation between results achieved by these two analytical methods.     

Experience of continuous fluorimetric monitoring of phytoplankton at a mooring station

The natural phytoplankton was monitored by means of fluorimetric equipment in Vostok Bay of the Sea of Japan. A gradual increase in the microalgae abundance was revealed in the course of the main water current, which enters the bay and leaves it. The continuous registration of chlorophyll fluorescence at a fixed point in the bay indicates the significant microscale variation of the abundance and functional state of the phytoplankton.     

Photoadaptation of carboxylating enzymes and photosynthesis during a spring bloom

Properties of the light saturation curve of photosynthesis and ribulose-1,5-bisphosphate carboxylase (RuBPC) activity are shown to change qualitatively in a natural population of marine phytoplankton during a spring bloom. Evidence is presented to show that these changes constitute photoadapative responses to increasing irradiance. As irradiance increased during the bloom, both the level of light-saturated photosynthesis (P_m) and the initial slope of the light saturation curve (α = photosynthetic efficiency) increased whether those parameters were normalized to chlorophyll a concentration (P_m^b, α^b) or to cell numbers (P_m^c, α^c). The magnitudes of these changes were such that I_k (= P_m/α, the photoadaptation parameter) did not change, but I_m, the light intensity at which photosynthesis becomes saturated, increased. RuBPC activity, both chlorophyll a (RuBPC^b) and cell number normalized (RuBPC^c), also increased during the bloom. We suggest that these adaptations were achieved by simultaneously increasing the number of photosynthetic units, proportionately decreasing the photosynthetic unit size, and increasing both the concentrations of the enzymes of the dark reactions and possibly also of photosynthetic electron transport components.We also observed diminished levels of photoinhibition in the high light adapted cells late in the bloom and have suggested that this was a consequence of the same suite of physiological changes.In situ carbon fixation per cell increased during the bloom whereas no change occurred in this parameter when normalized to chlorophyll a concentration. Although these photoadaptive responses thus permitted carbon to be fixed in situ more rapidly per cell, at a constant efficiency with respect to investment of energy in the photosynthetic apparatus, they did not result in a change in growth rate. Based on consideratios of the role of time scale in physiological adaptation, however, it is suggested that the observed alterations in photosynthesis with increasing irradiance might permit a cell to more rapidly fill an energy quota for division, possibly an advantage in a mixing environment in which energy is patchily distributed, both spatially and temporalyy.Phosphoenolpyruvate carboxylase activity when normalized to chlorophyll a (PEPC^b) did not change during the bloom while chlorophyll a normalized dark carbon fixation decreased sharply and was quantitatively small compared to PEPC^b. On this basis and considering that RuBPC^b increased during the bloom, it is suggested that, although PEPC may be involved in dark carbon fixation, its most important quantitative role is probably an indirect one in light dependent photosynthesis.We have also considered the relevance of laboratory results on photoadaptation to interpretations of field studies and have suggested that batch culture studies must be treated with caution but that turbidistat and semi-continuous methods provide reasonable simulations of natural conditions.