Deep pore water profiles reflect enhanced microbial activity towards tidal flat margins

Microbial activity in permeable tidal flat margin sediments is enhanced by two main processes. First, organic matter is supplied by rapid sedimentation at prograding tidal flat margins. Second, surface and deep pore water advection lead to a replenishment of the dissolved organic matter and sulfate pools. Increasing microbial activity towards the low water line is reflected in sulfate and methane profiles as well as in total cell numbers, sulfate reduction rates, and remineralization products. The impact of high sedimentation rates on pore water biogeochemistry is confirmed by inverse modeling reproducing the depth profiles obtained by measurements. In central parts of the tidal flats, low sedimentation rates and pore water flow velocities limit microbial activity despite the high availability of electron acceptors for microbial respiration such as sulfate. Therefore, tidal flat margins with high microbial activity are of special importance for budgeting biogeochemical cycling in tidal flat areas.     

Morphological controls in sandy estuaries: the influence of tidal flats and bathymetry on sediment transport

The morphodynamics of shallow, vertically well-mixed estuaries, characterised by tidal flats and deeper channels, have been investigated. This paper examines what contributes to flood/ebb-dominant sediment transport in localised regions through a 2D model study (using the TELEMAC modelling system). The Dyfi Estuary in Wales, UK has been used as a case study and, together with idealised estuary shapes, shows that shallow water depths lead to flood dominance in the inner estuary whilst tidal flats and deep channels cause ebb dominance in the outer estuary. For medium sands and with an artificially ‘flattened’ bathymetry (i.e. no tidal flats), the net sediment transport switches from ebb-dominant to flood-dominant where the parameter a/h (local tidal amplitude ÷ local tidally averaged water depth) exceeds 1.2. Sea level rise will reduce this critical value of a/h and also reduce the ebb-directed sediment transport significantly, leading to a flood-dominated estuarine system. A similar pattern, albeit with greater transport, was simulated with tidal flats included and also with a reduced grain size. This suggests that analogous classifications for flood/ebb asymmetry of the tide in estuaries as a whole may not represent the local sediment transport in sufficient detail. Through the Dyfi simulations, the above criterion involving a/h is shown to be complicated further by augmented flow past a spit at the estuary mouth which gives rise to a self-maintaining scour hole. Simulations of one year of bed evolution in an idealised flat-bottomed estuary, including tidal flow past a spit, recreate the flood/ebb dominance on either side of the spit and the formation of a scour hole in between. The erosion rate at the centre of the hole is reduced as the hole deepens, suggesting the establishment of a self-maintaining equilibrium state.     

Correlation between microbiological and chemical parameters of some hydrothermal springs in New Mexico, USA

Discharge areas of hydrothermal springs are known to be inhabited by diverse types of microorganisms including archaea, prokaryotes and eukaryotes. A total of 11 hydrothermal samples from the Rio Grande rift and the Valles caldera in New Mexico were analyzed to investigate the correlation between chemical and microbiological parameters of hydrothermal waters. The sampled fluids are categorized into three chemical groups: (I) steam-condensing acid sulfate waters, (II) deep geothermal and derivative waters and (III) thermal meteoric waters. Analyses of the microbial phospholipid fatty acids and denaturing gradient gel electrophoresis of DNA show that acid sulfate waters were populated by thermoacidophilic organisms and had high biomass content. Mineralized deep geothermal and derivative waters exhibited a high degree of microbial diversity, but had low biomass content. Thermal meteoric waters are low in total dissolved solids, and exhibit very low biomass content and microbial diversity. DNA sequences from several previously unknown microbial species were detected. The results of this study support the hypothesis that microbes can be used as tracers for specific types of subsurface environments.     

Tidal pumping and biogeochemical processes: Dissolution within the tidal capillary fringe of eogenetic coastal carbonates

Growing evidence suggests microbial respiration of dissolved organic carbon (DOC) may be a principal driver of subsurface dissolution and cave formation in eogenetic carbonate rock. Analyses of samples of vadose zone gasses, and geochemical and hydrological data collected from shallow, uncased wells on San Salvador Island, Bahamas, suggest tidally varying water tables may help fuel microbial respiration and dissolution through oxygenation. Respiration of soil organic carbon transported to water tables generates dysaerobic to anaerobic groundwater, limiting aerobic microbial processes. Positive correlations of carbon dioxide (CO₂), radon-222 (²²²Rn) and water table elevation indicate, however, that tidal pumping of water tables pulls atmospheric air that is rich in oxygen, and low in CO₂ and ²²²Rn, into contact with the tidal capillary fringe during falling tides. Ratios of CO₂ and O₂ in vadose gas relative to the atmosphere indicate this atmospheric oxygen fuels respiration within newly-exposed, wetted bedrock. Deficits of expected CO₂ relative to O₂ concentrations indicate some respired CO₂ is likely removed by carbonate mineral dissolution. Tidal pumping also appears capable of transferring oxygen to the freshwater lens, where it could also contribute to respiration and dissolution; dissolved oxygen concentrations at the water table are at least 5% saturated and decline to anaerobic conditions 1–2 m below. Our results demonstrate how tidal pumping of air to vadose zones can drive mineral dissolution reactions that are focused near water tables and may contribute to the formation of laterally continuous vuggy horizons and potentially caves. © 2020 John Wiley & Sons, Ltd.     

Seasonal dynamics of microbial sulfate reduction in temperate intertidal surface sediments: controls by temperature and organic matter

The role of microbial sulfate reduction on organic matter oxidation was studied quantitatively in temperate intertidal surface sediments of the German Wadden Sea (southern North Sea) on a seasonal base in the years 1998–2007. The sampling sites represent the range of sediments found in the back-barrier tidal area of Spiekeroog Island: sands, mixed and muddy flats. The correspondingly different contents in organic matter, metals, and porosities lead to significant differences in the activity of sulfate-reducing bacteria with volumetric sulfate reduction rates (SRR) in the top 15 cm of up to 1.4 μmol cm^?3 day^?1. Depth-integrated areal SRR ranged between 0.9 and 106 mmol m^?2 day^?1, with the highest values found in the mudflat sediments and lower rates measured in sands at the same time, demonstrating the impact of both temperature and organic matter load. According to a modeling approach for a 154-km² large tidal area, about 39, 122, and 285 tons of sulfate are reduced per day, during winter, spring/autumn, and summer, respectively. Hence, the importance of areal benthic organic matter mineralization by microbial sulfate reduction increases during spring/autumn and summer by factors of about 2 and 7, respectively, when compared to winter time. The combined results correspond to an estimated benthic organic carbon mineralization rate via sulfate reduction of 78 g C m^?2 year^?1.     

Biogeographical distribution and diversity of bacterial and archaeal communities within highly polluted anoxic marine sediments from the marmara sea

Physicochemical and microbiological characterization of anoxic sediments taken from seven highly polluted sites of the Marmara Sea was carried out. The 16S rRNA based microbial community structure analyses were performed using domain-specific PCR followed by denaturant gradient gel electrophoresis (DGGE) and sequencing of characteristic bands. The results showed that the microbial communities in these sediments were diverse and evenly distributed. Relating the prokaryotic and geochemical variables through statistical tools revealed that the microbial diversity in the sediments significantly related to depth, and S, Mn and Fe content of the sediments. Fermentative bacteria, denitrifying bacteria and hydrogenotrophic methanogens were dominant whereas sulfate reducing bacteria were absent in the DGGE patterns. This unusual microbial community structure implied that the newly discovered anaerobic methane oxidation coupled to denitrification process may occur in these subseafloor environments.     

Lunar seismicity and tectonics

Seismic signals from 300–700 deep moonquakes and about four shallow moonquakes are detected by the long-period seismometers of two or more of the Apollo seismic stations annually. Deep-moonquake activity detected by the Apollo seismic network displays tidal periodicities of 0.5 and 1 month, 206 d and 6 a. Repetitive moonquakes from 60 hypocenters produce seismograms characteristic of each. At each hypocenter, moonquakes occur only within an active period of a few days during a characteristic phase of the monthly lunar tidal cycle. An episode of activity may contain up to four quakes from one hypocenter. Nearly equal numbers of hypocenters are active at opposite phases of the monthly cycle, accounting for the 0.5-month periodicity. The 0.5- and 1-month activity peaks occur near times of extreme latitudinal and longitudinal librations and earth-moon separation (EMS). The 206-d and 6-a periodicities in moonquake occurrence and energy release characteristics are associated with the phase variations between the librations and EMS. Because of the exact relationship between tidal phases and the occurrence of deep moonquakes from a particular hypocenter, it is possible to predict not only the occurrence times from month to month, often to within several hours, but also the magnitudes of the moonquakes from that hypocenter. The predicted occurrence of large A₁ moonquakes in 1975, following a 3-a hiatus, confirms the correlation between A₁-moonquake activity and the 6-a lunar tidal cycle and implies a similar resurgence for all of the deep moonquakes. Because no matching shallow moonquake signals have been identified to date, tidal periodicities cannot be identified for the individual sources. However, shallow moonquakes generally occur near the times of extreme librations and EMS and often near the same tidal phase as the closest deep moonquake epicenters. With several possible exceptations, the deep-moonquake foci located to date occur in three narrow belts on the nearside of the moon. The belts are 100–300 km wide, 1,000–2,500 km long and 800–1,000 km deep and define a global fracture system that intersects in central Oceanus Procellarum. A fourth active, although poorly defined, zone is indicated. The locations of 17 shallow-moonquake foci, although not as accurate as the deep foci, show fair agreement with the deep-moonquake belts. Focal depths calculated for the shallow moonquakes range from 0–200 km. Deep-moonquake magnitudes range from 0.5 to 1.3 on the Richter scale with a total energy release estimated to be about 10¹¹ erg annually. The largest shallow moonquakes have magnitudes of 4–5 and release about 10¹⁵–10¹⁸ erg each. Tidal deformation of a rigid lunar lithosphere overlying a reduced-rigidity asthenosphere leads to stress and strain concentrations near the base of the lithosphere at the level of the deep moonquakes. Although tidal strain energy can account for the deep moonquakes in this model, it cannot account for the shallow moonquakes. The tidal stresses within the lunar lithosphere range from about 0.1 to 1 bar and are insufficient to generate moonquakes in unfractured rock, suggesting that lunar tides act as a triggering mechanism. The largest deep moonquakes of each belt usually occur near the same characteristic tidal phases corresponding to near minimum or maximum tidal stress, increasing tidal stress, and alignments of tidal shear stresses that correspond to thrust faulting along planes parallel to the moonquake belts and dipping 30–40°. With few exceptions, the shallow moonquakes occur at times of near minimum tidal stress conditions and increasing tidal stress that also suggest thrust faulting. The secular accumulation of strain energy required for the shallow moonquakes and implied by the uniform polarities of the deep moonquake signals probably results from weak convection. A convective mechanism would explain the close association between moonquake locations and the distribution of filled mare basins and thin lunar crust, the earth-side topographic bulge, and the ancient lunar magnetic field. The low level of lunar seismic activity and the occurrence of thrust faulting both at shallow and great depths implies that the moon is presently cooling and contracting at a slow rate.     

与地震有关的水文及地球化学变化

简要回顾了几十年来对地震发生前、地震过程中和震后地下流体和地球化学变化的研究和成果,这些研究一般都是以探索地震预报可能性为目的的。论述了与地震有关的地下水文及地球化学变化的机理,这些地下流体(包括地下水和气体诸如氢、氧和惰性气体)的起源和迁移流动现象以及详细介绍了早期和近代对有关地震的地下流体和地球化学变化的观测成果。同时指出了对地下流体和地球化学作为地震前兆来观测研究的困难所在以及为了克服这些困难而应该采取的地震前兆观测研究的方向,例如多种手段和多种原理方法,开发有效的地球物理和地球化学模型以及适当的数据分析统计方法等。     

Modified passive capillary samplers for collecting samples of snowmelt infiltration for stable isotope analysis in remote,seasonally inaccessible watersheds 2: field evaluation

Twelve modified passive capillary samplers (M‐PCAPS) were installed in remote locations within a large, alpine watershed located in the southern Rocky Mountains of Colorado to collect samples of infiltration during the snowmelt and summer rainfall seasons. These samples were collected in order to provide better constraints on the isotopic composition of soil‐water endmembers in the watershed. The seasonally integrated stable isotope composition (δ¹⁸O and δ²H) of soil‐meltwater collected with M‐PCAPS installed at shallow soil depths < 10 cm was similar to the seasonally integrated isotopic composition of bulk snow taken at the soil surface. However, meltwater which infiltrated to depths > 20 cm evolved along an isotopic enrichment line similar to the trendline described by the evolution of fresh snow to surface runoff from snowmelt in the watershed. Coincident changes in geochemistry were also observed at depth suggesting that the isotopic and geochemical composition of deep infiltration may be very different from that obtained by surface and/or shallow‐subsurface measurements. The M‐PCAPS design was also used to estimate downward fluxes of meltwater during the snowmelt season. Shallow and deep infiltration averaged 8·4 and 4·7 cm of event water or 54 and 33% of the measured snow water equivalent (SWE), respectively. Finally, dominant shallow‐subsurface runoff processes occurring during snowmelt could be identified using geochemical data obtained with the M‐PCAPS design. One soil regime was dominated by a combination of slow matrix flow in the shallow soil profile and fast preferential flow at depth through a layer of platy, volcanic rocks. The other soil regime lacked the rock layer and was dominated by slow matrix flow. Based on these results, the M‐PCAPS design appears to be a useful, robust methodology to quantify soil‐water fluxes during the snowmelt season and to sample the stable isotopic and geochemical composition of soil‐meltwater endmembers in remote watersheds. Copyright © 2009 John Wiley & Sons, Ltd.     

Assessing the spatial and temporal variability of dissolved organic matter in Liverpool Bay using excitation–emission matrix fluorescence and parallel factor analysis

The spatial and diurnal tidal variability of dissolved organic carbon (DOC) concentrations and the composition of dissolved organic matter (DOM), as evaluated by high-temperature catalytic oxidation and excitation–emission matrix combined with parallel factor analysis (EEM–PARAFAC), respectively, were determined in Liverpool Bay. EEM–PARAFAC modeling resulted in six fluorescent components characterized as terrestrial humic-like (two), microbial humic-like (two), and protein-like (two). The spatial distributions of DOC and the four humic-like components were negatively correlated with salinity in the high-salinity waters observed in this study (30.41–33.75), suggesting that terrestrial DOM was conservatively distributed. The spatial patterns of protein-like components were largely different from those of DOC, humic-like components, and chlorophyll a, suggesting that these distributions were the combined result of production and degradation in the bay in addition to river inputs. These findings suggest that the DOM dynamics in Liverpool Bay are strongly controlled by river-dominated allochthonous DOM inputs with some less significant contributions of autochthonous DOM within the bay. In addition, the temporal variations of DOM associated with the diurnal tidal cycles were determined at one inshore (31.34–32.24 salinity) and one offshore (33.64–33.75 salinity) station in the bay. Negative linear relationships between salinity and DOM characteristics, i.e., DOC, humic-like, and protein-like components, were observed at the inshore station. In contrast, no relationship was observed at the offshore station, suggesting that the export of DOM through rivers and possibly tidal flats have a noticeable influence on DOM concentration and composition up to a relatively elevated salinity of around 33 in Liverpool Bay.     

Geochemical heterogeneities and dynamics of magmas within the plumbing system of a persistently active volcano: evidence from Stromboli

We report here the most complete dataset for major and trace elements, as well as Sr isotopic compositions, of magmas erupted by Stromboli since the onset of present-day activity 1,800 years ago. Our data relate to both porphyritic scoria and lava originating in the uppermost parts of the feeding system, plus crystal-poor pumice produced by paroxysmal explosive eruption of deep-seated, fast ascending, magma. The geochemical variations recorded by Stromboli’s products allow us to identify changes in magma dynamics affecting the entire plumbing system. Deep-seated magmas vary in composition between two end-members having different key ratios in strongly incompatible trace elements and Sr isotopes. These features may be ascribed to mantle source processes (fluid/melt enrichment, variable degrees of melting) and occasional contamination by deep, mafic, cumulates. Temporal trends reveal three phases during which magmas with distinct geochemical signatures were erupted. The first phase occurred between the third and fourteenth centuries AD and was characterised by the eruption of evolved magmas sharing geochemical and Sr isotopic compositions similar to those of earlier periods of activity (<12 ka—Neostromboli and San Bartolo). The second phase, which began in the sixteenth century and lasted until the first half of the twentieth century, produced more primitive, less radiogenic, magmas with the lowest Ba/La and Rb/Th ratios of our dataset. The last phase is ongoing and is marked by a magma having the lowest Sr isotopic composition and highest Rb/Th ratio of the dataset. While this new magma can be clearly identified in the pumice erupted during the last two paroxysmal eruptions of 2003 and 2007, shallow degassed magma extruded during this time span records significant geochemical and isotopic heterogeneities. We thus suggest that the shallow reservoir has been only partially homogenised by this new magma influx. We conclude that compositional variations within the shallow magma system of a persistently active volcano provide only a biassed signal of ongoing geochemical changes induced by deep magma refilling. We argue that source changes can only be identified by interpreting the geochemistry of pumice, because it reliably represents magma transferred directly from deep portions of the plumbing system to the surface.     

Control of river stage on the reactive chemistry of the hyporheic zone

We examined the influence of river stage on subsurface hydrology and pore water chemistry within the hyporheic zone of a groundwater‐fed river during the summer baseflow period of 2011. We found river stage and geomorphologic environment to control chemical patterns in the hyporheic zone. At a high river stage, the flux of upwelling water in the shallow sediments (>20 cm) decreased at sample sites in the upper section of our study reach and increased substantially at sites in the lower section. This differential response is attributed to the contrasting geomorphology of these subreaches that affects the rate of the rise and fall of a river stage relative to the subsurface head. At sites where streamward vertical flux decreased, concentration profiles of a conservative environmental tracer suggest surface water infiltration into the riverbed below depths recorded at a low river stage. An increase in vertical flux at sites in the lower subreach is attributed to the movement of lateral subsurface waters originating from the adjacent floodplain. This lateral‐moving water preserved or decreased the vertical extent of the hyporheic mixing zone observed at a low river stage. Downwelling surface water appeared to be responsible for elevated dissolved organic carbon (DOC) and manganese (Mn) concentrations in shallow sediments (0–20 cm); however, lateral subsurface flows were probably important for elevated concentrations of these solutes at deeper levels. Results suggest that DOC delivered to hyporheic sediments during a high river stage from surface water and lateral subsurface sources could enhance heterotrophic microbial activities. Copyright © 2013 John Wiley & Sons, Ltd.     

Subsurface erosion,scarp retreat,and sedimentation at Mountain Lake,Virginia, USA: groundwater geomorphology in a flow‐through lake with subsurface drainage

The complete natural drainage in 2008, 2011, and 2012 of Mountain Lake in Giles County, Virginia, allowed detailed observations of the only natural lake basin in the southern Appalachian Mountains. Here we use these observations to support geomorphic analysis and develop a model of basin evolution, which may advance the understanding of rare flow‐through lakes with subsurface drainage elsewhere. Key features included (a) an angle‐of‐repose slope with a smoothly concave planform across the entire 260 m width of the north end of the basin, (b) an arc of steep‐sided depressions along the deep northern margin of the basin floor, and (c) an abrupt transition between colluvial and finer‐grained sedimentary deposits on the floor. Our geomorphic analysis suggests that subsurface erosion has enabled long‐term northward scarp retreat in the basin by removing water and sediment. Mountain Lake formed on the northern limb of a breached anticline along the Eastern Continental Divide, where strong‐over‐weak stratigraphy and a small watershed have enabled the basin to evolve generally as follows. (1) Pond Drain, a first‐order tributary of the New River, incised north‐dipping sandstones and underlying shales on the northern limb of the anticline. The valley floor subsequently accumulated meters to tens of meters of mostly late Pleistocene colluvial fill. (2) Subsurface drainage developed likely along the contact between the sandstones and shales, facilitated by pre‐existing fractures. (3) Ongoing subsurface erosion has progressively undermined the sandstone, causing scarp retreat along the northern margin of the basin while a surface stream intermittently incised the shallow southern end. Sedimentary deposits indicate that only the deeper northern portion of the basin is usually flooded under Holocene conditions. Our basin evolution model suggests slow development of the basin over hundreds of thousands of years rather than sudden damming by a catastrophic landslide. Copyright © 2018 John Wiley & Sons, Ltd.     

Impacts of Holocene and modern sea-level changes on estuarine mangroves from northeastern Brazil

Projections of the impacts of modern Relative Sea Level (RSL) rise on estuarine mangroves should be supported by coastal topographic data and records of mangrove dynamics under past RSL change. This work identified inland and seaward mangrove migrations along the Jucuruçu River (Bahia, Northeastern Brazil), during the Holocene based on sedimentary features, palynological and geochemical (δ¹³C, δ¹⁵N, C/N) data integrated with digital elevation models. During the Middle Holocene, in response to RSL rise, the estuary saw mangrove forest establish up to ~37 km inland. RSL stood between -1.4 (+0.36/-2.2 m) and +1 (2.19/0.2 m) around 7400 cal yr BP, and rose to a highest position of +3.25 (4.22/2.45 m) reached around 5350 cal yr BP. That marine incursion caused the inland replacement of freshwater vegetation by mangroves on tidal flats. Since then, the estuary experienced RSL fall, reducing inland tidal water salinity towards the Late Holocene, making that the mangroves were replaced by freshwater floodplain vegetation. Today, in the seaward part of the estuary near its mouth, mangroves occupy an area of ~10 km² along tidal channels. Considering a RSL rise of 98 cm up to the end of the 21^st century, at a rate significantly higher than that of Middle Holocene RSL rise (1.5 mm/yr) and fall (0.6 mm/yr), the current mangrove substrates are expected to drown and/or eroded near the coast, while new mangroves may establish inland, at topographically higher tidal flats in nowadays freshwater-tidal zones. Mangrove area could expand over 13 km² of coastal and flood plain. Following the same interaction between RSL/climate changes and Holocene mangrove dynamics, such upstream mangrove migration may be attenuated or intensified by changes in fluvial discharge. © 2019 John Wiley & Sons, Ltd.     

三河—平谷8.0级地震区地壳结构和活动断裂研究——利用单次覆盖深反射和浅层地震剖面

跨1679年三河—平谷8.0级地震区完成的单次覆盖深地震反射剖面和浅层地震反射剖面,揭示了三河—平谷地震区的地壳结构和断裂的深、浅构造特征.结果表明,该区地壳以TWT6~7 s左右的强反射带为界分为上地壳和下地壳,上地壳厚约18~21 km,下地壳厚约13~15 km.剖面揭示的地壳深断裂和浅部活动断裂具有上下一致的对应关系,其中,陡倾角的深断裂切割了下地壳和壳幔过渡带,向上延伸至上地壳,将地壳深部构造与浅部活动断裂联系在一起,这种深、浅共存的断裂构造体系是控制该区地震孕育和发生的重要因素,也是三河—平谷8.0级地震的深、浅构造背景.     

Indirect determination of the heat budget of tidal flats

A method is presented to calculate indirectly the heat budget of a tidal flat area from downstream observations of temperature and horizontal velocity in a tidal channel. It is only necessary to establish a relationship between the velocity and the volume flux. Then the heat budget of the upstream region is determined by integrating the heat flux over one tide. The proposed method is applied to long-term measurements obtained in 2004 at two sites in a tidal channel in the Hörnum Basin, German Wadden Sea. At the site located farther downstream in the channel, the upstream catchment area is diagnosed to export heat (heat gain in the interior) from March to August, while import is diagnosed for the same period of time at the other upstream site. From September to November the situation is reversed. An analytical estimate suggests that the sign of the budget is controlled by the tidal prism and the length of the dry-falling period of the flats in the respective upstream region. In addition, a simple model is developed which can be used to determine the integral bottom heat flux of the tidal flats.     

The role of wind in generating turbidity maxima in the Tay Estuary

Variations of wind direction and strength in the Tay Estuary control wave generation and the resultant patterns of suspended sediment concentration in the waters over the extensive tidal flats. Bodies of water with high water concentration peaks advect to occupy sites at which turbidity maxima are normally present at low water. Other low water peaks are generated by ebb tidal current resuspension.     

Using stable isotopes to identify major flow pathways in a permafrost influenced alpine meadow hillslope during summer rainfall period

Global warming has leaded to permafrost degradation, with potential impacts on the runoff generation processes of permafrost influenced alpine meadow hillslope. Stable isotopes have the potential to trace the complex runoff generation processes. In this study, precipitation, hillslope surface and subsurface runoff, stream water, and mobile soil water (MSW) at different hillslope positions and depths were collected during the summer rainfall period to analyse the major flow pathway based on stable isotopic signatures. The results indicated that (a) compared with precipitation, the δ²H values of MSW showed little temporal variation but strong heterogeneity with enriched isotopic ratios at lower hillslope positions and in deeper soil layers. (b) The δ²H values of middle-slope surface runoff and shallow subsurface flow were similar to those of precipitation and MSW of the same soil layer, respectively. (c) Middle-slope shallow subsurface flow was the major flow pathway of the permafrost influenced alpine meadow hillslope, which turned into surface runoff at the riparian zone before contributing to the streamflow. (d) The slight variation of δ²H values in stream water was shown to be related to mixing processes of new water (precipitation, 2%) and old water (middle-slope shallow subsurface flow, 98%) in the highly transmissive shallow thawed soil layers. It was inferred that supra-permafrost water levels would be lowered to a less conductive, deeper soil layer under further warming and thawing permafrost, which would result in a declined streamflow and delayed runoff peak. This study explained the “rapid mobilization of old water” paradox in permafrost influenced alpine meadow hillslope and improved our understanding of permafrost hillslope hydrology in alpine regions.     

Biodegradation in contaminated aquifers: incorporating microbial/molecular methods

In order to evaluate natural attenuation in contaminated aquifers, there has been a recent recognition that a multidisciplinary approach, incorporating microbial and molecular methods, is required. Observed decreases in contaminant mass and identified footprints of biogeochemical reactions are often used as evidence of intrinsic bioremediation, but characterizing the structure and function of the microbial populations at contaminated sites is needed. In this paper, we review the experimental approaches and microbial methods that are available as tools to evaluate the controls on microbially mediated degradation processes in contaminated aquifers. We discuss the emerging technologies used in biogeochemical studies and present a synthesis of recent studies that serve as models of integrating microbiological approaches with more traditional geochemical and hydrogeologic approaches in order to address important biogeochemical questions about contaminant fate.     

Degradation of crude oil in a contaminated tidal flat area and the resilience of bacterial community

Crude oil spills, Hebei Spirit in South Korea, is considered as one of the worst environmental disasters of the region. Our understanding on activation of oil-degrading bacteria and resilience of microbial community in oil contaminated sites are limited due to scarcity of such event. In the present study, tidal flat sediment contaminated by the oil spill were investigated for duration of 13 months to identify temporal change in microbial community and functional genes responsible for PAH-degradation. The results showed predominance of previously known oil-degrading genera, such as Cycloclasticus, Alcanivorax, and Thalassolituus, displaying significant increase within first four months of the accident. The disturbance caused by the oil spill altered the microbial community and its functional structures, but they were almost restored to the original state after 13 months. Present study demonstrated high detoxification capacity of indigenous bacterial populations in the tidal flat sediments and its resilience of microbial community.