抚仙湖垂向分层期间水体细菌群落结构组成及多样性的空间分布

水体垂向分层是深水湖泊一个重要的特征,这不仅影响水体理化环境因子,而且影响水生生物组成的垂向分布,但是对不同水层水体细菌的多样性和群落结构组成的形成与维持机制知之甚少.本研究以抚仙湖为研究对象,在水体热力分层期间采集不同位点、不同水层水样,借助16S rDNA Illumina Miseq高通量测序技术,探究水体细菌多样性和群落结构组成的空间分布特征及关键影响因子.研究结果表明:(1)抚仙湖北部湖心在水深15～40 m处出现温跃层,温度由23℃降至15℃,其余理化环境因子也存在明显的垂向分层现象;与垂向差异相比,表层水体各理化因子在水平方向上的差异相对较小.(2)在水平方向上,南部湖心表层水体细菌alpha多样性(Operational Taxonomic Unit数目和Faith’s phylogenetic diversity)最高;在垂向上,水体细菌alpha多样性随着采样水深的增加呈现单峰分布,在30和40 m水层最高;水体细菌alpha和beta多样性的垂向差异均显著大于水平方向差异.(3)表层水体细菌主要由Actinomycetales、unidentified Cyano...     

Plankton community as an indicator of water quality in tropical shrimp culture ponds

The plankton was examined as an indicator of water quality in 14 shrimp Litopenaeus vannamei farms in Brazil in 2003. The ponds were categorized by high stocking density (>30 PL m⁻²) of phytoplankton, consisting of 51 species with concentrations ranging from 365,218 ± 416,615 cells mL⁻¹ to 1,961,675 ± 3,160,172 cells mL⁻¹. Diatoms contributed to almost 70% of the species number and high densities resulted from Cyanophyta blooms, mainly Pseudanabaena cf limnetica. Forty zooplankton taxa were registered and were essentially composed of typical marine euryhaline species and suspension-feeders. Copepoda dominated (45%) the make-up, followed by Protozoa (18%), Rotifera (12%), and Mollusca (12%) larvae. Zooplankton varied from 972 ± 209 ind m⁻³ to 4235 ± 2877 ind m⁻³. Enhanced nutrient input affected plankton density and composition. Diatom and Copepoda dominance was replaced by cyanobacteria, protozoan, and rotifers as nutrient concentrations increased with the cultured period, indicating that plankton structure is affected by eutrophic conditions.     

南京玄武湖浮游细菌群落结构的季节变化及其与环境因子的关系

应用16S rRNA基因末端限制性片段长度多态性技术和多元统计方法,探究了南京玄武湖浮游细菌群落结构的季节性变化,并找出了浮游细菌群落组成分布的主要影响因子.结果表明,玄武湖4个季节中夏季Invsimpson指数、Shannon-Wiener指数和Pielou指数最高,而春季最低.非度量多维尺度分析结果显示浮游细菌群落结构存在着明显的季节性变化.Mantel检验、典范对应分析、变量分割分析表明影响玄武湖细菌群落结构最显著的环境因子为温度,其次为pH.营养盐浓度对玄武湖细菌群落结构的影响不显著.总的来说,本研究将有助于更好地理解富营养化水体中的浮游细菌群落组成的季节变化规律.     

Environmental drivers of tadpole community structure in temporary and permanent ponds

About 87 % of the anuran species that occur in Brazil possess a larval stage, i.e., tadpoles. Throughout this stage of development, tadpoles are subject to predation and the conditions of their aquatic habitat, including environmental heterogeneity, physical and chemical properties of the water, and hydroperiod of the water body. We assessed the patterns of species composition and richness of tadpoles in permanent and temporary ponds, with the objective of evaluating how morphometric, physical and chemical factors of the ponds and environmental heterogeneity (surrounding vegetation and substrate cover and aquatic vegetation) affect anuran occupation of these environments. The study was performed in five permanent and four temporary ponds. Tadpoles of 20 anuran species differed in species composition between permanent and temporary environments. Environmental factors were significant drivers for tadpole richness: turbidity and dissolved oxygen were significantly related to richness in permanent ponds, while water temperature and pH were significantly correlated to richness in temporary environments. Higher species richness was related to the heterogeneity of the ponds and greater complexity associated with the stratification of the surrounding vegetation. In general, the results suggest that species sorting of tadpoles is probably related to the high environmental variation among permanent and temporary aquatic habitats. The spatial heterogeneity of these two types of environments increases local diversity by accommodating species that are unique to only one habitat type.     

珠江下游浮游细菌群落结构的时空分布特征

浮游细菌群落对河流变化具有高度响应性,并可能影响河流生境的生物地球化学过程.因此,了解浮游细菌群落的时空特征,阐明其在河流生态系统中的生态功能具有重要科学意义.于2016年6月和12月对珠江下游浮游细菌群落结构的时空特征进行调查研究,并采用16S rRNA高通量测序技术对样品组分进行分析.结果表明细菌群落主要由变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、抗微菌门(Verrucomicrobia)和蓝细菌门(Cyanobacteria)组成.其中变形菌门相对丰度最高,主要包括β-变形菌纲(Betaproteobacteria)、α-变形菌纲(Alphaproteobacteria)和γ-变形菌纲(Gammaproteobacteria).季节上,丰水期Shannon-Wiener和Chao 1多样性指数高于枯水期其中芽孢杆菌纲(Bacilli)和黄杆菌纲(Flavobacteriia)差异显著;空间上,珠江下游可分为西江沿线、珠三角河网中部和广州市周边3个区域.采用RDA分析...     

不同养殖类型池塘浮游生物群落结构的初步分析

1988年在山东省禹城县渍涝洼地中选择四口鱼塘以不同放养类型进行试验,4—10月测定其浮游生物数量、生物量的变化。根据浮游生物各主要大类(如原生动物、轮虫以及蓝藻、绿藻、硅藻、隐藻和裸藻等)的变化,分析不同养殖类型池塘浮游生物群落结构变化的稳定性和同步性,同时分析了环境因子特别是生物营养物质的变化对浮游生物的影响。结果指出,肥水性鱼类在养殖池塘中因搭配比例的差异而导致池塘中浮游生物变化。     

Phytoplankton community composition in nearshore coastal waters of Louisiana

Phytoplankton community compositions within near-shore coastal and estuarine waters of Louisiana were characterized by group diversity, evenness, relative abundance and biovolume. Sixty-six taxa were identified in addition to eight potentially harmful algal genera including Gymnodinium sp. Phytoplankton group diversity was lowest at Vermillion Bay in February 2008, but otherwise ranged between 2.16 and 3.40. Phytoplankton evenness was also lowest at Vermillion Bay in February 2008, but otherwise ranged between 0.54 and 0.77. Dissolved oxygen increased with increased biovolume (R2 = 0.85, p < 0.001) and biovolume decreased with increased light attenuation (R2 = 0.34, p = 0.007), which supported the importance of light in regulating oxygen dynamics. Diatoms were dominant in relative abundance and biovolume at almost all stations and all cruises. Brunt-V?is?l? frequency was used as a measure of water column stratification and was negatively correlated (p = 0.02) to diatom relative percent total abundance.     

大型浅水湖泊中浮游细菌群落的空间格局及其驱动机制——以太湖为例

浮游细菌在驱动湖泊物质循环、指示湖泊水环境质量等方面发挥重要作用,探明其在湖泊中的空间分布格局及其驱动机制,是揭示湖泊物质循环过程及水环境状况的关键。浅水湖泊具有水动力扰动强烈的生境特点,其中的浮游细菌群落空间格局的形成机制尚未明晰。本研究以大型浅水湖泊——太湖为研究对象,通过对全湖开展系统的野外调查,结合高通量测序技术、多元统计分析方法及生态学模型构建,系统探明太湖浮游细菌的空间分布格局,并揭示格局形成的驱动机制。结果显示：（1）太湖西北部湖区（藻型湖区）与东南部湖区（草型湖区）之间浮游细菌群落结构存在显著差异,而这两个区域内各湖区间及湖心区与周边湖区间之间的浮游细菌群落结构差异不显著,太湖浮游细菌群落结构整体上呈现较弱的距离衰减规律;（2）太湖草、藻型湖区及湖心区中浮游细菌群落结构分别与叶绿素a、透明度及总悬浮物显著相关,总体上环境变量对太湖浮游细菌群落空间格局的相对贡献率大于空间变量;（3）尽管确定性过程中的异质化选择、随机过程中的非主导性过程以及扩散限制对太湖浮游细菌空间格局的形成均产生重要影响,但总体上随机过程对格局形成的相对贡献更大;（4）太湖无序风场驱动的水力混合作用导致...     

菹草腐解期上覆水浮游细菌群落动态模拟及其环境驱动因子分析

菹草生长期内可以从水体中吸收大量的营养物质,有效抑制水体富营养化。但在季节交替期,菹草衰亡后,其残体腐解会向上覆水释放大量营养物质,对湖泊生态系统造成瞬时冲击。通过实验室模拟,将菹草分别浸泡在不同稀释比的上覆水中自然腐解,旨在研究菹草残体腐解过程上覆水水质变化及环境因子对浮游细菌的驱动作用。结果表明:(1)在稀释组(B、C组)中,菹草腐解的氮素均出现了短期冲击(第1天),冲击程度与稀释比呈正相关。(2)上覆水水质越好,浮游细菌共有OTUs(operational taxonomic units,操作分类单元)数量占比越高,且浮游菌群结构受氮素冲击的变化越剧烈。实验第30天时,稀释湖水4倍组(C组)共有OTUs占比为61%,高于原水组(A组,OTUs占比40%)和稀释2倍组(B组,OTUs占比45%)。群落结构的剧烈变化主要通过影响厚壁菌门(Firmicutes)、疣微菌门(Verrucomicrobiota)、放线杆菌门(Actinobacteriota)和蓝藻菌门(Cyanobacteria)的相对丰度大小实现。(3)变形杆菌门(Proteobacteria)是整个腐解过程中的第一大...     

Carbon isotopic composition and its implications on paleoclimate of the underground ancient forest ecosystem in Sihui,Guangdong

We present the carbon isotopic composition of the total organic carbon (TOC) and fine roots in the sedimentary profile from the underground ancient forest in Sihui to study the climatic and environmental changes from 4.5 ka BP to 0.6 ka BP. Results show that C₃ plant was the main vegetation from 4.5 ka BP to 0.6 ka BP in this region. The ancient forest began to develop in the wetland at around 4 ka BP and disappeared together with the wetland at about 3.0 ka BP, implying that the climate had changed greatly at around 3.0 ka BP. As indicated by the simulation results, the content of atmospheric CO₂ increased slightly during 3.5 ka BP to 3.0 ka BP, implying climate warming during that period. The interval of radiocarbon age between 3.0 ka BP to 1.2 ka BP was possibly caused by the strong erosion when the block was lifted in the neotectonic movement. From 1.2 ka BP to 0.6 ka BP, the region remained in terrestrial sedimentary environment, and the surface plant biomass declined gradually. Drought caused by the climate change was the likely cause for the disappearance of the ancient forest. South transition of Intertropical Convergence Zone (ITCZ) was probably the main mechanism for the climate change. Supported by National Natural Science Foundation of China (Grant Nos. 40231015 and 40473002), National Basic Research Program of China (Grant No. 2005CB422004), the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KSCX2-SW-133) and Open Funds of State Key Laboratory of Organic Geochemistry (Grant No. OGL-200607)     

扎龙国家级自然保护区硅藻植物特征及其环境指示作用

2011年春、夏、秋季对扎龙国家级自然保护区内的硅藻群落结构进行研究.通过鉴定,共发现硅藻门植物112个分类单位,包括96种16变种,隶属于2纲6目9科32属.通过对硅藻植物的种类组成、聚类分析等方法,对扎龙国家级自然保护区内的硅藻植物分布情况进行初步研究,结果表明,扎龙国家级自然保护区内硅藻植物种类较为丰富,保护区内水体普遍为受轻污染或中度污染状态,个别水域存在富营养化情况,春、夏、秋季节中,秋季的富营养情况较为严重.结合水体的理化指标,通过对扎龙国家级自然保护区水体中硅藻的优势种、指示种的分析,发现该水域喜碱性、半咸水种类及中污带的种类较多,说明保护区水体受到轻微的污染,个别水域出现富营养状态.扎龙国家级自然保护区水体呈中-弱碱性、微盐,表明该区域受到了土地盐碱化的影响.     

Patterns in the linkage of water quantity and quality during low‐flows

This study investigates 72 catchments across the federal state of Baden‐Wuerttemberg, Germany, for changes in water quality during low‐flow events. Data from the state's water quality monitoring network provided seven water quality parameters (water temperature, electrical conductivity, concentrations of chloride, sodium, sulfate, nitrate, and phosphate), which were statistically related to streamflow variability. Water temperature changes during low‐flow showed seasonal dependence. Nitrate concentrations revealed high spatial heterogeneity with about one third of the stations showing decreasing values during low discharge. For most other parameters, concentrations increased during low‐flow. Despite consistent trend directions, the magnitudes of changes with streamflow differed markedly across the state. Both multiple linear regression and a multiple analysis of variances were applied to explain these differences with the help of catchment characteristics. Results indicated that for sulfate and conductivity, geology of the catchments was the most important control, whereas for chloride, sodium, and nitrate, sewage treatment plants had the largest influence. For phosphate, no clear control could be identified. Independent from the applied method, land use was a less important control on river water quality during low‐flow than geology or inflow from sewage treatment plants. These results show that the effects of diffuse and point sources, as well as those of natural and anthropogenic sources differ for different water quality parameters. Overall, a high diversity of potential water quality deterioration signals needs to be considered when the ecological status of rivers is to be protected during low‐flow events.     

Carbon isotope composition and its implications of Lower Cretaceous Aptian-Albian shallow water carbonates in the Cuoqin Basin, North Tibet

Since Schlanger and Jenkyns (1976)[1] first ad-vanced the ?oceanic anoxic events ?(OAEs) model to explain the origin of the worldwide distributed Early Cretaceous Aptian-Albian (115—103 MaBP) and Late Cretaceous Cenomanian-Turonian (95—88 MaBP) black shales, the OAEs and responses to them have been one of the 揾ot spots?in geological circles. Up till now, researchers at home and abroad have exten-sively studied the sedimentary and geochemical re-sponses to the two anoxic events, espe…     

珠江三角洲养殖池塘底栖动物群落结构及水质生物学评价

珠江三角洲是我国主要的水产养殖区域之一.为了解珠江三角洲鱼类养殖池塘底栖动物群落结构,进而评价养殖水体水质状况,于2016年7-8月对6种养殖模式30口池塘底栖动物及其环境因子进行调查.结果表明:1)共采集底栖动物18种,隶属于3门14属,其中寡毛类和水生昆虫均为7种,软体动物为4种.优势种为克拉泊水丝蚓(Limnodrilus claparedeianus)、水丝蚓属一种(Limnodrilus sp.)和摇蚊属一种(Chironomus sp.)等耐污种类.2)6种养殖模式池塘底栖动物平均种类数无显著差异,但其密度和生物量却存在显著差异,杂交鳢Y(杂交鳢幼鱼)和杂交鳢C(杂交鳢成鱼)养殖池塘均显著高于其他4种养殖模式池塘.3)皮尔逊相关分析结果表明,影响珠江三角洲池塘底栖动物密度和生物量的主要因素是总氮(TN)和总磷(TP).两个主要分类群中,寡毛类密度和生物量仅与TN浓度呈显著正相关,而摇蚊幼虫却与TN和TP浓度均呈显著正相关.4)采用Shannon-Wiener多样性指数、Margalef多样性指数和Biotic Index生物指数对6种养殖模式30口池塘水质进行评价,结果表明Shannon-Wiener多样性指数不宜应用于珠江三角洲池塘的水质评价,Margalef多样性指数和Biotic Index生物指数的评价结果均表明杂交鳢Y、杂交鳢C以及草鱼池塘全部处于重度污染,而大口黑鲈S(投喂饲料)、大口黑鲈S+B(投喂饲料+冰鲜鱼类)和罗非鱼池塘大部分处于重度污染,少数处于中度污染.     

Water quantity and quality simulation by improved SWAT in highly regulated Huai River Basin of China

High regulation of dams or sluices disturbs flow regimes and pollutant transformation process significantly in most basins over the world. The water quality and quantity simulation in highly regulated river basins is always a very complicated task. The Huai River Basin is a typical area in China with the highest density of water projects and serious pollution problems simultaneously. In this paper, a procedure based on Soil and Water Assessment Tool (SWAT) was proposed to carry out the water quantity and quality simulation of Huai River Basin by incorporating the operation rules of dams or sluices into the reservoir regulation module. The water quality module in SWAT is extended to suit the actual situation of river basin in China. The results show that: for runoff simulation, 28 of all the 38 stations (73.7%) have the acceptable performance, with the average correlation coefficient and efficiency coefficient of 0.83 and 0.66, respectively in the calibration period. In the validation period, 17 of all the 34 stations (50%) have the acceptable performance, with the average correlation coefficient and efficiency coefficient of 0.77 and 0.54, respectively. The model performs the worst for reservoirs, little better for sluices and the best for unregulated stations. Comparing to the low flow and high flow simulation of original SWAT model, the low flow simulation of reservoirs and the high flow simulation of sluices are much better because of their different main purposes. For water quality simulation, the standard reaching rates of NH₃–N and COD_Mn are 55.9% (19/34) and 67.6% (23/34) respectively for all the stations, and the average relation coefficients are 0.46 and 0.48 respectively. Comparing with the results of original SWAT model, the improved model better reproduces the long-term water quantity and quality processes in the Huai River Basin of China. This study provides a new approach and reference to understand the variation of water quantity and quality in highly regulated river basin, and is expected as technical support for the environment restoration and integrated management in the basins, especially in China.     

岱海水体及沉积物细菌多样性及群落组成特征

采用高通量测序技术,研究了内蒙古岱海流域入湖河流、湖水及沉积物细菌多样性及群落组成.结果显示,细菌多样性从高到低依次为：沉积物>河流>湖泊.聚类分析表明入湖河流、湖水和沉积物细菌群落可分为明显不同的3支,说明这3种生境中细菌群落结构有较大差异.物种注释结果表明,河流中优势细菌菌群为髌骨细菌(Patescibacteria)、变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和放线菌门(Actinobacteria);湖水中优势细菌菌群为放线菌门(Actinobacteria);而沉积物中优势细菌菌群为变形菌门和绿弯菌门(Chloroflexi).典范对应分析及Monte Carlo检验表明,电导率和悬浮物含量对水体中(河流与湖泊)细菌群落影响显著,二者共解释了细菌群落变化的86.5%;而冗余分析及Monte Carlo检验表明,泥深、磁化率和总有机碳对沉积物中细菌群落影响显著,三者共解释了细菌群落变化的47.9%.近30年来,岱海地区气候变化和人类活动导致湖水咸化,沉积物碳氮指标显著增长.岱海水体及沉积物细菌多样性及群落组成的差异及其主要驱动因...     

Seasonal description of the quality and quantity of snowmelt in a mountainous region using an integrated model

Abstract

An integrated model for the simulation and prediction of both snowmelt quantity and quality from seasonal snow cover in a mountain basin has been developed at the Institute of Hydrology and Hydraulics SAS in cooperation with the Geological Institute of Dionyz ?tur. The model will be used for both the simulation and forecast of snow accumulation and the accumulation of SO^2- ₄ washout. At the present time it is mostly used for simulation of the SO^2- ₄ concentration and water supply in snow. In this paper the results of SO^2- ₄ modelling in a brook are also presented.     

青藏高原热融湖塘的水热过程及其对下伏多年冻土的热影响

以青藏高原查拉坪地区一处热融湖塘(40 m×50 m,最大深度为1 m)为研究对象,由实测数据对比分析了热融湖塘与天然地表相同深度的温度变化特征.结果表明:与天然地表相比,热融湖塘融化时间长,冻结时间短,且存在接近4℃的水温变化;受太阳辐射及热对流的影响,垂向水温梯度仅在水表从4℃降温及冻结阶段较大,其余时段接近0;湖底年均温度比相同深度的天然地表高约6.4℃,湖底下部存在约14 m深随时间发展的融区,土体吸热增大,放热减小;热融湖塘2.5~3.0 m土体的年内热交换为19592.0 k J/m2,约是天然地表的230倍,其中吸热量及放热量分别为后者的1.4倍及8.7%.湖塘下部的融化夹层是深层冻土的主要热源,湖塘对下部土体放热的抑制作用是湖塘对土体产生热影响的主要原因.     

沉水植物生态修复对西湖细菌多样性及群落结构的影响

沉水植物的重建是湖泊富营养化修复的关键措施之一.本研究利用在杭州西湖进行的沉水植物生态修复工程,应用基于聚合酶链式反应的变性梯度凝胶电泳(PCR-DGGE)及分子克隆等手段,研究种植菹草和苦草对水体中细菌多样性及群落结构的影响.未进行沉水植物生态修复的湖区细菌以Bacteroidetes(42.9%)、Betaproteobacteria(30.8%)和Verru-comicrobia(14.3%)为主.而种植菹草和苦草后,Bacteroidetes所占的比例分别减少到12.7%和5.3%;Betaproteobacteria则分别提高到52.4%和59.5%;Alphaproteobacteria由4.4%分别提高到19.0%和12.8%.种植菹草后Verrucomicrobia所占比例大幅降低,而种植苦草对Verrucomicrobia的影响不大.沉水植物修复不仅可以改善水质,同时也可以显著提高水体中细菌的多样性,改变细菌的群落结构.