光照及营养盐对3种赤潮甲藻吞噬营养行为的影响

通过在室内批量培养实验中添加活体藻类及荧光标记藻类(Fluorescent Labled Algae,FLA)的方式,研究不同光照及营养盐条件下3种赤潮甲藻米氏凯伦藻(Karenia mikimotoi)、链状亚历山大藻(alexandrium catenella)及东海原甲藻(Prorocentrum donghai...     

网状原角藻化感作用的初步研究

为明确海洋甲藻——网状原角藻(Protoceratium reticulatum)对海洋微藻的化感作用,揭示其化感物质作为植物源抑藻剂的开发潜能,本文探讨了网状原角藻藻细胞和无细胞滤液对几种海洋微藻——东海原甲藻(Prorocentrum donghaiense)、拟旋链角毛藻(Chaetoceros pseudocurvisetus)、米氏凯伦藻(Karenia mikimotoi)和赤潮异弯藻(Heterosigma akashiwo)生长的影响,并进一步选取东海原甲藻和赤潮异弯藻作为受体藻种,以塔玛亚历山大藻(Alexandrium tamarense)作为阳性对照,比较了2种甲藻的藻细胞培养液和无细胞滤液对东海原甲藻、赤潮异弯藻生长的影响差异。通过比较发现,网状原角藻的无细胞滤液比塔玛亚历山大藻能更明显的抑制东海原甲藻和赤潮异弯藻的生长,尤其是对东海原甲藻,基本阻断其生长。     

东海米氏凯伦藻水华中中华哲水蚤的选择性摄食

提要为评估中型浮游动物选择性摄食对有害藻华发展进程的影响,应用一种新的结合Frost直接摄食法和Landry稀释法的现场培养方法,于2005年4月27日—6月5日在东海有害藻华高发区的6个典型站位进行了中华哲水蚤(Calanus sinicus)对浮游植物和微型浮游动物摄食速率的研究。比较了中华哲水蚤对米氏凯伦藻(Karenia mikimotoi)和具齿原甲藻(Prorocentrum dentatum)摄食习性的差异,并评估了其摄食在水华进程中的作用。研究结果表明,中华哲水蚤对有害藻华物种存在摄食选择性和摄食速率的阈值。当自然水体中米氏凯伦藻细胞丰度达到157cells/ml和具齿原甲藻细胞丰度达到981 cells/ml时是中华哲水蚤由偏好趋于排斥摄食的阈值。当自然水体中米氏凯伦藻细胞丰度达到176 cells/ml时,中华哲水蚤对其停止摄食。米氏凯伦藻有害藻华发生区中华哲水蚤对具齿原甲藻的无选择性滤食以及对米氏凯伦藻的排斥摄食行为,影响水华进程,最终导致水华物种向米氏凯伦藻方向演替。     

两种甲藻对人工海水中胶体磷的利用初探

采用批次培养方法,研究了东海原甲藻(Prorocentrum donghaiense)和链状亚历山大藻(Alexandrium catenlla)对胶体磷的生物可利用性,并初步探讨了甲藻利用胶体磷的机制问题.结果表明东海原甲藻和链状亚历山大藻均能利用胶体磷生长繁殖.东海原甲藻在接种11d后细胞密度在无机磷和胶体磷培养基中分别为10.53×107和3.43×107个/dm3.链状亚历山大藻在接种11d后细胞密度在无机磷和胶体磷培养基中分别为39.0×105和28.3×105个/dm3.通过对比细胞密度,胶体磷对东海原甲藻生长的促进作用要低于无机磷的作用;胶体磷对链状亚历山大藻生长的促进作用与无机磷的作用相当.东海原甲藻和链状亚历山大藻在胶体磷源下碱性磷酸酶活性迅速升高,前期均显著高于各自无机磷组的碱性磷酸酶活性,碱性磷酸酶活力最高值分别为0.29和0.30μmol/(dm3·h).初步结果表明,两种甲藻均能通过碱性磷酸酶的降解来利用胶体磷.     

NH_4-N氮源下海洋酸化对东海原甲藻和米氏凯伦藻生长的影响

本文选取东海原甲藻和米氏凯伦藻,以氨氮(NH_4-N)为氮源,通过增加pCO_2(800和1 500μatm)改变海水的酸化程度,研究海洋酸化对东海原甲藻和米氏凯伦藻生长以及抗氧化生物酶活性的影响。研究结果表明,海洋酸化对东海原甲藻和米氏凯伦藻的生长均有抑制作用,两种藻的生长速率在酸化条件下均低于正常海水条件。东海原甲藻和米氏凯伦藻的最大氮吸收速率及其与半饱和常数的比值(V_(max)/K_s比率)均随着酸化程度的加深而降低,表明酸化抑制了浮游植物对NH_4-N的吸收。东海原甲藻在三个实验条件下的V_(max)/K_s比率均高于米氏凯伦藻,表明东海原甲藻比米氏凯伦藻有更强的环境适应性。海洋酸化诱导了东海原甲藻和米氏凯伦藻的活性氧(ROS)升高,随着酸化程度加深,抗氧化生物酶—超氧化歧化酶(SOD)和过氧化氢酶(CAT)活性升高,这说明,海洋酸化对两种甲藻生长的抑制,通过抗氧化酶的活性变化在生理层面对外界环境作出响应,与生长和营养盐吸收过程相对应。     

东海大规模赤潮危害影响的实验研究——对黑褐新糠虾和卤虫的毒性效应

近年来,东海长江口邻近海域连年爆发覆盖面积广、持续时间长的大规模东海原甲藻(Prorocentrum donghaiense)赤潮(最高密度达107 cells/mL)(Zhou,2001),在某些区域,链状亚历山大藻(Alexandrium catenella)赤潮(最高密度达105cells/mL)也伴随发生(周名江等,2003)。2005年还发生了大面积的米氏凯伦藻(Karenia mikimotoi)赤潮(孙军等,2006)。作者针对赤潮危害的形成机制和进一步了解大规模赤潮的发生对整个海洋生态的影响展开了系列研究。黑褐新糠虾(Neomysis awatschensis)是广泛分布于我国沿海的一种小型甲壳生物,多生活于近岸水域,为广温…     

东海原甲藻与塔玛亚历山大藻种群增长过程与种间竞争研究

针对我国东海2种重要赤潮肇事生物东海原甲藻(Prorocentrum donghaiense)和塔玛亚历山大藻(Alexan-drium tamarense)进行了种群增长过程和种间竞争的实验研究。结果表明,单种培养时,不同氮磷比对东海原甲藻和亚历山大藻种群增长过程没有明显的影响,但是会影响其最终的细胞密度;在混合培养时,营养盐组成比例不同对东海原甲藻和塔玛亚历山大藻的种间竞争过程有影响,但不会影响其最后竞争结果。竞争物种的细胞起始密度对种间竞争结果有明显的影响,P.donghaiense和A.tamarense的接种细胞密度比为2 000∶100和2 000∶200时,东海原甲藻占优势;当接种细胞密度比为4 000∶400时,塔玛亚历山大藻占优势。     

适合海洋微藻活体染色的方法评价

为了评估一种快速简单用以确定海洋微藻细胞活性的技术,针对船舶压载水中常见的3个门类中11种10～50μm单细胞微藻用中性红(NR)、5-氯甲基荧光素二乙酸酯(CMFDA)、荧光素二乙酸酯(FDA)三种染料进行染色,通过光镜和荧光显微镜对染色结果进行测定。结果表明,NR(中性红)是检测本实验中全部海洋微藻藻株细胞活性的最佳染料,染色最佳浓度为1/10 000,染色时间为30min;5-氯甲基荧光素二乙酸酯(CMFDA)、荧光素二乙酸酯(FDA)和双荧光染色对海洋微藻藻株活细胞着色时间短,染色效果明显,但其应用具有局限性,适用于检测本实验中甲藻门(塔玛亚历山大藻、链状亚历山大藻、微小原甲藻、利玛原甲藻、东海原甲藻、米氏凯伦藻)和绿藻门(青岛大扁藻和杜氏盐藻)的活性,染色最佳浓度为5μmol/L FDA+2.5μmol/L CMFDA,染色时间为10min,但不适用于检测硅藻门的细胞活性。因此,中性红更适合检测船舶压载水中微藻活细胞,根据光镜下微藻细胞着色情况而判断细胞活性。     

混合培养条件下几种赤潮藻对无机磷源的竞争生长研究

采用批次培养方式,研究混合培养的几种赤潮藻东海原甲藻(PrDrocentrum donghaiense)与中肋骨条藻(Skeletonema costatum)及东海原甲藻与链状亚历山大藻(Alexandrium catenella)对溶解无机磷源的竞争生长响应.结果表明,在富磷及贫磷的培养条件下,中肋骨条藻的比生长率远超过东海原甲藻,而成为培养体系中的绝对优势种.在东海原甲藻与链状亚历山大藻的混合培养体系中,原甲藻大量死亡,可能存在亚历山大藻对原甲藻的他感作用.培养体系中,碱性磷酸酶活性随藻类磷胁迫而显著升高,可能在后期种群利用代谢有机磷源时发挥重要作用,且在不同培养体系中表现出酶活性大小及状态的差异性,该结果可能影响浮游植物对有机磷源的利用效率.     

罗源湾口柱状沉积物中的甲藻孢囊

通过对福建罗源湾口海域KMZK5柱状沉积物中甲藻孢囊的分析,共鉴定出15属30种甲藻孢囊。对比发现这30种甲藻孢囊是该湾以前未被记录的种类。其中12种是附近海域也未曾发现的种类,6种为有毒种类:缘亚历山大藻、小型亚历山大藻、塔玛亚历山大藻、具刺膝沟藻、链状裸甲藻、锥状斯氏藻.同时对甲藻孢囊的主要属种和有毒种类的丰度、分布在垂直方向上的变化特征进行了初步研究.     

东海大规模赤潮危害影响的实验研究——对黑褐新糠虾和卤虫的毒性效应

The effects of Prorocentrum donghaiense,Karenia mikimotoi,Alexandrium catenella,the causative species of Harmful Algal Blooms in the East China Sea,on the survival and growth of Neomysis awatschensis and Artemia salina were investigated.And the toxicity of eleven strains of Alexandrium----A.lusitanicum(AL-1)、A.minutum(AM-1)、A.spp.(A-K)、A.tamarense(ATHK,ATCI02,ATCI03,AT5-1,AT5-3,AT-6)、A.affine(AS-1,AC-1) to the two above organisms were compared. The results showed that P.donghaiense and K.mikimotoi had no adverse effect on the survival and growth of N.awatschensis and A.salina at 96h.However,the survival rate of N.awatschensis and A.salina declined linearly with the increasing density of A.catenella,and the 96h LC₅₀ for N.awatschensis and A.salina was 4500 and 460 cells/mL,respectively, Five strains of Alexandrium----A.spp,A.lusitanicum (AL-1),A.tamarense(AT5-3,AT-6) and A.affine (AS-1)at the density of 4000cells/mL and 96h had no adverse impacts on N.awatschensis. But other six strains of Alexandrium a.Tamarense(AT5-1,ATHK,ATCI02,ATCI03),A.affine(AC-1) and A.minutum(AM-1)decreased the survival fate of N.awatschensis significantly. Two strains of Alexandrium----A.lusitanicum(AL-1) and A.tamarense(AT-6),at the density of 500cells/mL. had no adverse effects on A.salina in 96h;while the other Alexandrium strains all had adverse effeets on the survival of a.salina, A.salina could increase its length with the increasing density of P.donghaiense,K.mikimotoi and A.luxitanicum. The results indicate that P.donghaiense and K.mikimotoi have nio significant effect on the survival of crustaeean zoop lankton in a short period. But several Alexandrium specised/strains could influence the survival of crustacean zooplankton likely by non-PSP substances. A.salina is shown to be more sensitive than N.awatschensis as test organisms to harmful species.     

大鹏湾几种赤潮甲藻的分类学研究

对采自深圳大鹏湾的５种赤潮甲藻进行了分类学研究。其中海洋原甲藻属ｐｒｏ－ｒｏｃｅｎｔｒｕｍ２种：Ｐ．ｍｉｃａｎｓ Ｅｈｒｅｎｂｅｒｇ，Ｐ．ｍｉｎｉｍｕｍ（Ｐａｖｉｌｌａｒｄ）Ｓｃｈｉｌｌｅｒ；亚历山大属Ａｌｅｘａｎｄｒｉｕｍ２种：Ａｔａｍａｒｅｎｓｅ（Ｌｅｂｏｕｒ）Ｂａｌｅｃｈ，Ａ．ｃａｔｅｎｅｌｌａ（Ｗｈｅｄｏｎ＆Ｋｏｆｏｉｄ）Ｂａ－ｌｅｃｈ；施克里普藻属Ｓｃｒｉｐｐｓｉｅｌｌａ１种：Ｓ．ｔｒｏ     

东海原甲藻线粒体细胞色素b(Cytb)基因的定量检测

建立了检测东海原甲藻线粒体细胞色素b(Cytb)基因mRNA含量的实时荧光定量PCR方法.以甲藻Cytb基因的简并引物扩增得到984 bp长的东海原甲藻Cytb基因片段,经克隆、测序,制备并纯化质粒.以光度法测定质粒浓度并作为标准品.对上述基因片段,利用PRIMER EXPRESS 3.0 设计引物,以梯度稀释的质粒标准品进行定量PCR反应,以SYBR Green I为荧光染料,制作标准曲线,得到基因拷贝数X与Ct值的关系为:Ct=-3.50 lg X+39.35(相关系数r为0.999).通过对不同生长时期的东海原甲藻样品的Cytb基因mRNA含量检测,发现该基因的表达量较稳定,平均值为(45.4±4.7)拷贝/细胞,受生长状态影响不大,可作为实时荧光定量PCR的内参基因.     

Co-monitoring bacterial and dinoflagellates communities by denaturing gradient gel electrophoresis(DGGE) and SSU rRNA sequencing during a dinoflagellates bloom

Dinoflagellates are unicellular eukaryotic protists that dominate in all coastal waters, and are also present in oceanic waters. Despite the central importance of dinoflagellates in global primary production, the relationship between dinoflagellates and bacteria are still poorly understood. In order to understand the ecological interaction between bacterial and dinoflageUates communities, denaturing gradient gel electrophoresis (DGGE) and SSU rRNA sequencing were applied to monitoring the population dynamics of bacteria and dinoflagellates from the onset to disappearance of a dinoflagellates bloom occurred in Baltimore Inner Harbor, from April 15 to 24, 2002. Although Prorocentrum minimum was the major bloom forming species under the light microscopy, DGGE method with dinoflagellate specific primers demonstrated that Prorocentrum micans, Gymnodinium galatheanum and Gyrodinium uncatenum were also present during the bloom. Population shifts among the minor dinoflagellate groups were observed. DGGE of PCR-amplified 16S rRNA gene fragments indicated that cyanobacteria, α, β, γ-proteobacteria, FlavobacteriumBacteroides-Cytophaga (FBC), and Planctomcetes were the major components of bacterial assemblages during the bloom. DGGE analysis showed that Cytophagales and α-proteobacteria played important roles at different stages of dinoflagellates bloom. DGGE can be used as a rapid tool to simultaneously monitor population dynamics of both bacterial and dinoflagellates communities in aquatic environments, which is demonstrated here.     

The effect of the causative algae of large-scale HAB in the East China Sea on egg hatching of Argopecten irradians, and population growth of Brachionus plicatilis and Moina mongolica

The impacts of Prorocentrum donghaiense Lu and Alexandrium catenella Balech, causative species of the large-scale HAB in the East China Sea, were studied under laboratory conditions. According to bloom densities, the effects of monoculture and mixture of the two species were examined on the egg-hatching success of Argopecten irradians Lamarck, and the population growth of Brachionus plicatilis Müller and Moina mongolica Daday. The results showed that monoculture of A. catenella had a significant inhibition on the egg hatching success of A. irradians, and the population growth of B. plicatilis and M. mongolica. The median effective densities (ED_50) inhibiting the egg hatching success of A. irradians for 24 h and the population growth of B. plicatilis and M. mongolica for 96 h were 800, 630, and 2 400 cells/cm3, respectively. Monoculture of P. donghaiense has no such inhibitory effect on the egg hatching success of A. irradians; P. donghaiense at lower suitable densities could sustain the population growth of B. plicatilis (1×104~3×104cells/cm3) and M. mongolica (2×104~5×104cells/cm3); P. donghaiense at higher densities had significantly adverse effect on the population growth of B. plicatilis (4×104~10×104cells/cm3) and M. mongolica (10×104cells/cm3). When the two algae were mixed according to bloom densities, P. donghaiense at suitable densities to some extent could decrease the toxicity of A. catenella to B. plicatilis and M. mongolica. The results indicated that the large-scale HAB in the East China Sea could have adverse effect on zooplankton, and might further influence the marine ecosystem, especially when there was also Alexandrium bloom.     

2010—2020年宁德近岸海域赤潮时空分布特征分析

文章收集整理2010—2020年发生在宁德近岸海域的赤潮事件,综合分析该海域赤潮的时空分布和种类特征,结果表明：(1)近11年来宁德近岸海域共发生赤潮16起,年均1.5起;总面积高达1 468 km²,年均133.5 km²;持续时间共169天,年均15.4天。(2)从月份上看,赤潮发生在4—6月,高发期为5月。(3)发生赤潮的种类共有3种甲藻和1种硅藻,其中发生次数、面积和持续天数最高的均为东海原甲藻,其次为米氏凯伦藻,最低为中肋骨条藻。(4)从空间分布上来看,福宁湾及其周边海域(A区)赤潮次数和持续天数最长,三沙湾外海域(D区)赤潮面积最大,三沙湾内海域(C区)的赤潮种类最多。(5)米氏凯伦藻引发的有毒赤潮共4次,主要集中在霞浦近岸海域。适宜的温度、盐度、丰富的营养盐和良好的天气是米氏凯伦藻形成赤潮的前提条件。     

Identification of dissolved and particulate carbonyl compounds produced by marine harmful algal bloom species

Carbonyl compounds, especially polyunsaturated aldehydes (PUAs), are increasingly reported as teratogenic to grazers and deleterious to phytoplankton. While PUAs have been considered to be mainly produced by diatoms after cell wounding, little is known about whether microalgae other than diatoms, particularly harmful algal bloom (HAB)-forming species, produce carbonyl compounds. In this study, we analyzed the carbonyl compounds from eight common HAB-forming species ( Akashiwo sanguinea , Karenia mikimotoi , Karlodinium venefi cum , Margalefi dinium polykrikoides , Prorocentrum donghaiense , P . minimum , Scrippsiella trochoidea , and Heterosigma akashiwo ) using gas chromatography-mass spectrometry in full scan and selected ion monitoring (SIM) modes. Our results show a ubiquitous presence of carbonyl compounds in both dissolved and particulate forms in all species we examined. In the full scan mode, 133 chromatographic peaks were detected from 51 samples altogether. Both the varieties of carbonyl compounds and their quantities were algal species dependent, although most of the carbonyl compounds could not be fully identifi ed according to the mass spectral database only due to the unavailability of enough standards for all analytes. Aided with nine standards in SIM mode, we further identifi ed and quantifi ed all nine aldehydes (2-methyl-2-pentenal, trans-2-nonenal, cis-6-nonenal, 2,6-dimethyl-5-heptenal, trans-2-hexenal, trans-2- decenal, 2,4-heptadienal, trans-trans-2,4-octadienal, and trans-trans-2,4-decadienal). Four of these nine aldehydes were detected in particulate form, which confi rmed that trans-2-nonenal could be produced by K . mikimotoi , Karl . venefi cum , P . donghaiense , P . minimum , S . trochoidea , and H . akashiwo , 2,4-heptadienal and trans-trans-2,4-decadienal by A . sanguinea , M . polykrikoides , and S . trochoidea , and trans, trans-2,4- octadienal by S . trochoidea , respectively. We proved that some dinofl agellate and raphidophyte species could contribute to the pool of carbonyl compounds including PUAs in a marine ecosystem. Some carbonyl compounds, particularly those in high cell quota and/or dissolved concentration, may be related to fi shkilling or allelopathy which needs further identifi cation and quantifi cation.     

海洋原甲藻与三角褐指藻混合培养条件下的种群生长与氮磷营养盐变化

以三角褐指藻(Phaeodactylum tricornutum Bohl)和海洋原甲藻(Prorocentrum mi-cans)为实验种研究了硅藻与甲藻在混合培养的环境中其各自的种群密度变化以及相应水体中氮磷营养盐变化。结果显示,在单独培养或与海洋原甲藻共同培养的条件下,三角褐指藻均表现出明显的营养盐吸收优势,种群生长迅速。相对而言,海洋原甲藻对营养盐的吸收速率明显较低,而且在与三角褐指藻共同培养的水体中一直处于竞争劣势;但有海洋原甲藻存在的水体中,三角褐指藻较单独培养时具有更高的生长速率和高的生物量,海洋原甲藻的存在可能促进三角褐指藻的生长。     

几株原甲藻核糖体大亚基RNA基因的部分克隆及序列分析

对7株赤潮原甲藻28S rDNA 5'端部分序列进行扩增、克隆和序列测定,并从GeneBank上获取14个原甲藻28S rDNA序列,用NJ法和ME法构建了原甲藻属的系统树,并对序列进行分析.结果表明,7株原甲藻28S rDNA扩增序列长度为950~958 bp,通过NJ法和ME法构建的系统树完全一致.大部分分离自不同海域的同种原甲藻的序列高度保守,而不同种间在序列高变区却有较大的差异.但来自南海海域的海洋原甲藻(Prorocentrum micans)与分离自其他海域的株系序列差异较大,甚至超出了有些种间的差异.由28S rDNA高变区获得的序列,有望成为浮游植物特异性分子探针设计的良好靶区域.     

福建沿岸海域主要赤潮生物的生态学特征

福建沿岸海域是中国的赤潮多发海区之一.据统计,1962～2008年间,福建沿岸海域共发生赤潮180起以上,潜在的赤潮生物有124种,已经引发过赤潮的种类有27种.本文对中肋骨条藻（Skeletonema costatum）、角毛藻（Chaetocerosspp.）、夜光藻（Noctiluca scintillans）、东海原甲藻（Proro-centrum donghaiense）、米氏凯伦藻（Karenia mikimotoi）、裸甲藻（Gymnodinium spp.）和球形棕囊藻（Phaeocystis globosa）等福建沿岸海域主要赤潮生物的生态学特征和发生原因进行了分析和探讨,评述了福建沿岸海域赤潮发生的特点.福建沿岸海域引发赤潮频率最高的赤潮生物是夜光藻,米氏凯伦藻造成的水产养殖损失最大.其赤潮多发季节在春夏季的4～7月.其赤潮多发区主要分布在3个区域：宁德沿岸海域（以四礵列岛为中心）、厦门西港海域和平潭沿岸海域.对福建沿岸海域几种主要赤潮生物的研究表明,赤潮的发生与水温、盐度、气象、水动力、营养盐等环境条件密切相关.